characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000803) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 13 minutes, starting at 10:45 and ending at 10:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000803


status: PUBLIC

tag: attribute

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

unit: DD

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

unit: practical salinity unit

characteristic: Salinity Sensor

tag: Namespace:PANGAEA Data Publisher for Earth and Environmental Science

characteristic: Submitter Id

unit: m

characteristic: depth

text: This sample (EMOSE_N010000804) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 10:45 and ending at 10:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

unit: ºC

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000804

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000805) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 9 minutes, starting at 10:49 and ending at 10:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000805


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000806) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 10:50 and ending at 10:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000806

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000808) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 11:15 and ending at 11:22 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000808


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000817) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 11:53 and ending at 11:59 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000817

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000809) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 8 minutes, starting at 11:22 and ending at 11:30 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000809


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000810) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 11:15 and ending at 11:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000810

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000811) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 9 minutes, starting at 11:15 and ending at 11:24 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000811


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000812) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 11:24 and ending at 11:29 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000812

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000813) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 14 minutes, starting at 11:30 and ending at 11:44 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000813


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000814) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 11:30 and ending at 11:36 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000814

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000815) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 11:37 and ending at 11:44 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000815


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000816) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 3 minutes, starting at 11:41 and ending at 11:44 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000816

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000818) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 10 minutes, starting at 11:48 and ending at 11:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000818


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000827) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 8 minutes, starting at 12:34 and ending at 12:42 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000827

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000819) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 10 minutes, starting at 11:58 and ending at 12:08 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000819


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000820) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 12:09 and ending at 12:14 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000820

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000821) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 11 minutes, starting at 12:09 and ending at 12:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000821


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000822) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 12:09 and ending at 12:15 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000822

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000823) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 10 minutes, starting at 12:16 and ending at 12:26 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000823


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000824) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 12:18 and ending at 12:23 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000824

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000825) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000826,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 13 minutes, starting at 12:24 and ending at 12:37 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000825


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000826) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000824,EMOSE_N010000820,EMOSE_N010000819,EMOSE_N010000823,EMOSE_N010000822,EMOSE_N010000825,EMOSE_N010000821,EMOSE_N010000827,EMOSE_N010000817,EMOSE_N010000815,EMOSE_N010000814,EMOSE_N010000818,EMOSE_N010000813,EMOSE_N010000811,EMOSE_N010000809,EMOSE_N010000808,EMOSE_N010000812,EMOSE_N010000810,EMOSE_N010000816,EMOSE_N010000807,EMOSE_N010000806,EMOSE_N010000799,EMOSE_N010000798,EMOSE_N010000805,EMOSE_N010000804,EMOSE_N010000802,EMOSE_N010000800,EMOSE_N010000803,EMOSE_N010000801

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 15-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 12:26 and ending at 12:33 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000826

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000425) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was not size-fractionated and prepared back in the lab using protocol [IMG-FCM_W>0.22] for later detection and identification of biological entities (organisms) by flow cytometry imaging (IMG) methods. This sample may be used for example in biodiversity studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000426,EMOSE_N010000427

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole IMG-FCM_W>0.22, the sample material was not pre-filtered. A volume of 0.0015 Litres was packaged in a sterile 2-mL cryotube, treated with the addition of 15 microLitres of cold 25% glutaraldehyde and 150 microLitres of 10% paraformaldehyde, labelled with a barcode identification sticker, flash-frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 2: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 3: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 4: The sample was sent for later analysis to the Observatoire Oc�anologique de Banyuls sur Mer (OOB), France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_IMG-FCM_W>0.22_N010000425


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000681) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was not size-fractionated and prepared back in the lab using protocol [IMG-ILM_W>0.22] for later detection and identification of biological entities (organisms) by microscopy and imaging (IMG) methods. This sample may be used for example in biodiversity studies of eukaryotes. This sample has replicate sample(s): EMOSE_N010000682,EMOSE_N010000683

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole IMG-ILM_W>0.22, the sample material was not pre-filtered. A volume of 0.05 Litres was packaged in a sterile 50-mL falcon tube, treated with the addition of 5 mL of 10% paraformaldehyde, labelled with a barcode identification sticker, and stored in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 2: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 3: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 4: The sample was sent for later analysis to the Observatoire Oc�anologique de Banyuls sur Mer (OOB), France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_IMG-ILM_W>0.22_N010000681

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000426) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was not size-fractionated and prepared back in the lab using protocol [IMG-FCM_W>0.22] for later detection and identification of biological entities (organisms) by flow cytometry imaging (IMG) methods. This sample may be used for example in biodiversity studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000425,EMOSE_N010000427

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_IMG-FCM_W>0.22_N010000426