E-MTAB-6535:Sample 1

characteristic: ENA-CHECKLIST

text: Center for Biotechnology (CeBiTec)

text: public

text: E-MTAB-6535:Sample 1

tag: Namespace:Center for Biotechnology (CeBiTec)

text: ArrayExpress

characteristic: broker name

text: Protocols: The colony was maintained in a circulating artificial seawater system at approximately 26 °C with 20-40 µmol photons m-2s-1 (T5 light) of photosynthetically active radiation on a 10:14 h light-dark cycle. Total RNA was isolated using 30 mg of tissue in a 2 ml tube with 2500 µl TRIzol (Life Technologies, Carlsbad, USA) and then incubated for one hour. Further tissue disruption and homogenization was performed with grinding beads in 2 ml tubes using a TissueLyser II (QIAGEN) for three 1-min cycles. Total RNA was purified from the homogenized sample with the Direct-Zol RNA miniprep (Zymo, Irvine, USA) following manufacture’s protocol. In short, 2500 µl of ethanol (100%) were added to the sample and transferred to a Zymo-Spin IIC Column and centrifuged at 6000 rpm for 3 minutes. Then, 400 μl of Direct-zol RNA PreWash were added to the column for centrifugation and 700 μl RNA Wash Buffer were added to the column. A further centrifugation at 6000 rpm for 1 minute was performed and the column was placed in a new RNase-free tube. The sample was then eluted following Zymo's instructions. The quantity and quality of RNA sample was assessed using a RNA Pico chip on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). To obtain the hologenome sequence data, a whole-genome-shotgun PCR-free paired-end (Nextera DNA Sample Prep Kit; Illumina, San Diego, USA) and a 10 kbp mate-pair libraries (Nextera Mate Pair Sample Preparation Kit; Illumina) were generated based on the manufacturer’s protocol.

text: adult

characteristic: developmental stage

text: Indonesia

characteristic: environmental history

text: wild type genotype

characteristic: genotype

text: circulating seawater at 26 degrees C on a 14:10 hour light cycle

characteristic: growth condition

text: Porites rus

text: Porites rus

text: Porites rus

text: Sample 1


status: PUBLIC

text: Staphylococcus aureus

tag: attribute

characteristic: ArrayExpress-SPECIES

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Staphylococcus aureus

characteristic: common name

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: subject id

characteristic: title


characteristic: ENA-CHECKLIST

text: ISTITUTO SUPERIORE DI SANITA

text: public

tag: Namespace:ISTITUTO SUPERIORE DI SANITA

characteristic: collection date

text: Italy

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: faeces

characteristic: isolation source

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri SF1


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCL Cancer Institute

characteristic: INSDC center name

text: public

tag: Namespace:UCL Cancer Institute

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: smoked in the past

characteristic: clinical history

text: human

text: Protocols: Blood samples (4 ml) were taken by a medical doctor at the UCL Hospital using EDTA Vacutainers (Becton Dickinson). All samples were processed and stored at the UCL/UCLH Biobank for Studying Health and Disease (http://www.ucl.ac.uk/silva/biobank/ucl-hta-biobank/ucl-hta-biobanks-cancer) using HTA-approved standard operating procedures (SOPs) RNA sequencing (RNA-seq) was carried out on RNA extracted from blood using both targeted and whole RNA-seq. Targeted RNA-seq was conducted using AmpliSeq Whole Transcriptome on the Ion Proton System (ThermoFisher) and whole RNA-seq was carried using on HiSeq 4000 (Illumina). The targeted RNA-seq library preparation was carried out using AmpliSeq (Thermo Fisher Scientific) which is designed to profile over 21,000 distinct human RNA targets using a highly multiplexed amplification method. Each amplicon represents a unique targeted gene. The average size of each amplicon is ~150 bp. For library preparation, a barcoded cDNA library was first generated with SuperScript VILO cDNA Synthesis kit from 20 ng of total RNA treated with Turbo DNase (Thermo Fisher Scientific). Then cDNA was amplified using Ion AmpliSeq technology to accurately maintain expression levels of all targeted genes. Amplified cDNA Libraries were evaluated for quality and quantified using Agilent Bioanalyzer high sensitivity chip. Libraries were then diluted to 100 pM and pooled equally, with two individual samples per pool. Pooled libraries were amplified using emulsion PCR on Ion Torrent OneTouch2 instruments (OT2) and enriched following manufactures instructions. Templated libraries were then sequenced on an Ion Torrent Proton sequencing system, using Ion PI kit and chip version 2. For whole RNA-seq, the libraries were prepared with Illumina-compatible SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, NH, USA) according to the manufacturer’s protocol. 20 ng of total RNA (quantified using Qubit fluorometer) was taken for library preparation. Poly(A) mRNA selection was performed using Oligo dT beads. The poly(A) mRNA was subjected to reverse transcription, ligation followed by second strand synthesis. The double-stranded library was purified to remove magnetic beads and second strand synthesis reaction components. The library was amplified for 18 cycles of PCR to add the complete adaptor sequences and indexes. The PCR conditions were as follows: Initial denaturation at 98°C for 30s, cycling of 98°C for 10s, 65°C for 20s and 72°C for 30s, and final extension at 72°C for 1min). The final PCR product (sequencing library) was purified using SPRI (Solid Phase Reversible Immobilisation) beads followed by library quality control check. The Illumina-compatible sequencing library was quantified using Qubit fluorometer (Thermo Fisher Scientific) and its fragment size distribution was analysed on Bioanalyzer 2100 (Agilent). All libraries had a fragment length distribution range between 400bp to 450bp. Libraries were further quantified by qPCR using KAPA library quantification kit for Illumina (Kapa Biosystems).

characteristic: description

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

text: UCL Cancer Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: never smoked

text: human

characteristic: common name

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCL Cancer Institute

characteristic: INSDC center name

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characteristic: age

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text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male


text: UCL Cancer Institute

text: public

text: ArrayExpress

characteristic: broker name

text: smoked occasionally

characteristic: clinical history

text: human

characteristic: description

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male


text: gut metagenome

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characteristic: ENA-CHECKLIST

text: SC

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text: public

text: gut metagenome

text: gut metagenome

text: gut metagenome

characteristic: subject id


text: INSTITUTE OF EVOLUTIONARY BIOLOGY

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: Submitter Id

text: Normal diet

text: Daphnia magna

characteristic: organism

text: Daphnia magna


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

text: public

text: Caloric restricted diet

text: Daphnia magna

text: Daphnia magna

characteristic: title


text: FRIEDRICH SCHILLER UNIVERSITY JENA

characteristic: INSDC center name

text: public

tag: Namespace:FRIEDRICH SCHILLER UNIVERSITY JENA

characteristic: collection date

text: DNA-based analysis

text: limestone aquifers

characteristic: environment (biome)

text: limestone aquifers

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

text: AquaDiva

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: bacterial diversity in groundwater

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FRIEDRICH SCHILLER UNIVERSITY JENA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DNA-based analysis

characteristic: description

text: soil

text: forest soil

text: seepage water

text: Germany

text: mimarks-survey

text: unidentified bacterium

characteristic: organism

text: AquaDiva

text: unidentified bacterium

text: Illumina MiSeq

text: bacterial diversity in groundwater

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRIEDRICH SCHILLER UNIVERSITY JENA

characteristic: INSDC center name

text: public

characteristic: collection date

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characteristic: environment (biome)

text: forest soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: mimarks-survey

text: unidentified bacterium

text: AquaDiva

text: unidentified bacterium

text: Illumina MiSeq

text: bacterial diversity in groundwater

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident63642206

tag: Namespace:Georgia State University

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

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text: public

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text: HamsterExperimentNANAhomecageresident63442206

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text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

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text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


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text: terrestrial biome

characteristic: environment (biome)

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characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident62342206

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

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characteristic: investigation type

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characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

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characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

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text: HamsterExperimentNANAhomecageresident62842206

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

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characteristic: title


characteristic: ENA-CHECKLIST

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text: golden hamster

characteristic: common name

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text: terrestrial biome

text: animal-associated habitat

text: feces

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characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

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characteristic: investigation type

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characteristic: project name

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

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text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

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text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

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text: Georgia State University

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characteristic: collection date

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characteristic: environment (biome)

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characteristic: environment (feature)

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characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

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characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecagecontrol62642206

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characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

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characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

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characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

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characteristic: project name

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text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


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characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: SRA accession

text: HamsterExperimentNANAhomecagecontrol62742206

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident63642208

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident63442208

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident62242208

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident62342208

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident63842208

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident62842208

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident63242208

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident64142208

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


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characteristic: External Id

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident61642208

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecagecontrol62942208

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecagecontrol62642208

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecagecontrol62442208

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecagecontrol64242208

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecagecontrol62742208

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident63642212

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident63442212

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident62242212

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident62342212

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident63842212

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident62842212

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecageresident63242212

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecageresident64142212

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecageresident61642212

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecagecontrol62942212

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

text: public

text: HamsterExperimentNANAhomecagecontrol62642212

text: golden hamster

characteristic: common name

text: KatieProject

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mesocricetus auratus

text: KatieProject

characteristic: project name

text: Mesocricetus auratus

text: Illumina MiSeq

characteristic: sequencing method

text: KatieProject


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Georgia State University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HamsterExperimentNANAhomecagecontrol62442212

characteristic: Submitter Id

text: golden hamster

text: KatieProject

characteristic: description

text: terrestrial biome

text: animal-associated habitat

text: feces

text: USA

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

characteristic: organism

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Georgia State University

characteristic: INSDC center name

text: public

text: HamsterExperimentNANAhomecagecontrol62742212

characteristic: collection date

text: golden hamster

text: KatieProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: Mesocricetus auratus

text: KatieProject

text: Mesocricetus auratus

text: Illumina MiSeq

text: KatieProject

characteristic: title


text: Wildtype

characteristic: ArrayExpress-PHENOTYPE

text: male

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

characteristic: common name

text: Male

characteristic: gender

text: Mus musculus

text: Wildtype

characteristic: phenotype

text: Mus musculus

characteristic: subject id


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRA (INRA Montpellier)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:INRA (INRA Montpellier)

characteristic: Submitter Id

text: woodland

text: branch

text: branch Pinus strobus

text: Canada

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: Cinara strobi

characteristic: organism

text: Aphididae 16S rRNA gene sequencing

characteristic: project name

text: Cinara strobi

text: Illumina

characteristic: sequencing method

text: Cinara strobi 16S data


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF HELSINKI

characteristic: INSDC center name

text: public

tag: Namespace:UNIVERSITY OF HELSINKI

characteristic: collection date

text: marine

characteristic: environment (biome)

text: pancake ice

characteristic: environment (feature)

text: sea ice

characteristic: environment (material)

text: Yes

characteristic: environmental sample

text: Antarctica

characteristic: geographic location (country and/or sea)

text: uncultured eukaryote

text: fwd_seq: CGGTAAYTCCAGCTCYV, rev_seq: AYTTRAAGDAATTGACGG

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF HELSINKI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine

text: sea ice

text: sea ice

text: Yes

text: Antarctica

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

text: uncultured eukaryote


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF HELSINKI

characteristic: INSDC center name

text: public

characteristic: collection date

text: marine

characteristic: environment (biome)

text: sea ice

characteristic: environment (feature)

text: slush ice

text: Yes

text: Antarctica

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote


text: UNIVERSITY OF HELSINKI

text: public

text: marine

text: sea ice

text: Yes

characteristic: environment (material)

text: Yes

characteristic: environmental sample

text: Antarctica

characteristic: geographic location (country and/or sea)

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF HELSINKI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine

text: sea ice

text: brine

text: Yes

text: Antarctica

text: uncultured eukaryote

text: uncultured eukaryote


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF HELSINKI

characteristic: INSDC center name

text: public

characteristic: collection date

text: marine

characteristic: environment (biome)

text: water

text: Yes

text: Yes

text: Antarctica

text: -62.000 -

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

text: uncultured eukaryote


text: UNIVERSITY OF HELSINKI

text: public

text: marine

text: water

characteristic: environment (feature)

text: Yes

characteristic: environment (material)

text: Yes

characteristic: environmental sample

text: Antarctica

characteristic: geographic location (country and/or sea)

text: uncultured eukaryote

characteristic: organism

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene


CECT 8504

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UVEG

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: CECT 8504

text: water

text: water

text: Matapan Vavilov deep canyon in the Eastern Mediterranean sea

text: Italy

characteristic: geographic location (depth)

text: mimarks-survey

characteristic: investigation type

text: Palleronia abyssalis

text: Whole genome sequencing of Palleronia abyssalis CECT 8504, type strain

characteristic: project name

text: Palleronia abyssalis

text: Illumina Miseq

characteristic: sequencing method

text: CECT 8504

characteristic: strain

text: Palleronia abyssalis CECT 8504

characteristic: title

text: water

characteristic: water environmental package


text: Mycobacterium tuberculosis

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Mycobacterium tuberculosis

characteristic: common name

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

characteristic: subject id


CECT 8599

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UVEG

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: CECT 8599

characteristic: collection date

text: host associated

characteristic: environment (biome)

text: golden sea squirt (Halocynthia aurantium)

characteristic: environment (feature)

text: golden sea squirt ( Halocynthia aurantium) was collected from the East Sea at Yangyang

characteristic: environment (material)

text: South Korea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: Ascidiaceihabitans donghaensis

characteristic: organism

text: Whole genome sequencing of Ascidiaceihabitans donghaensis CECT 8599, type strain

text: Ascidiaceihabitans donghaensis

text: Illumina Miseq

text: CECT 8599


CECT 8626

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UVEG

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: CECT 8626

text: water

text: water

text: ocean meets a freshwater spring in Soesokkak

text: South Korea

characteristic: geographic location (depth)

text: mimarks-survey

characteristic: investigation type

text: Defluviimonas aquaemixtae

text: Whole genome sequencing of Defluviimonas aquaemixtae CECT 8626, type strain

characteristic: project name

text: Defluviimonas aquaemixtae

text: Illumina Miseq

characteristic: sequencing method

text: CECT 8626

characteristic: strain

text: Defluviimonas aquaemixtae CECT 8626

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: DUKE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Plants were field collected and tissue was frozen with Liquid nitrogen before RNA extraction Stranded mRNA-seq

characteristic: description

text: gametophyte

characteristic: developmental stage

text: Sphagnum cribrosum

text: Sphagnum cribrosum

text: whole organism

characteristic: organism part

text: Sphagnum cribrosum

text: NFHL


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DUKE

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: gametophyte

text: Flatbergium novo-caledoniae

characteristic: organism

text: Flatbergium novo-caledoniae

text: whole organism

text: Flatbergium novo-caledoniae


text: DUKE

text: public

text: ArrayExpress

text: gametophyte

text: Eosphagnum inretortum

text: Eosphagnum inretortum

text: whole organism

text: Eosphagnum inretortum

characteristic: title


text: DUKE

text: public

text: ArrayExpress

text: gametophyte

text: Flatbergium sericeum

text: Flatbergium sericeum

text: whole organism

text: Flatbergium sericeum


CECT 8811

text: UVEG

text: public

text: CECT 8811

characteristic: collection date

text: water

characteristic: environment (biome)

text: seawater

characteristic: environment (feature)

text: Seawater was collected from off Geoje Island in the South Sea, South Korea

characteristic: environment (material)

text: South Korea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Aliiroseovarius pelagivivens

text: Whole genome sequencing of Aliiroseovarius pelagivivens CECT 8811, type strain

characteristic: project name

text: Aliiroseovarius pelagivivens

text: Illumina Miseq

characteristic: sequencing method

text: CECT 8811

characteristic: strain

text: Aliiroseovarius pelagivivens CECT 8811

text: water

characteristic: water environmental package


CECT 8812

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UVEG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: CECT 8812

characteristic: Submitter Id

text: water

text: seawater

text: South Korea

text: mimarks-survey

text: Pontivivens insulae

text: Whole genome sequencing of Pontivivens insulae CECT 8812, type strain

text: Pontivivens insulae

text: Illumina Miseq

text: CeCT 8812

text: Pontivivens insulae CECT 8812

text: water


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY HOSPITAL TUEBINGEN

text: splenocytes

characteristic: cell type

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: tissue type

text: WT#1 IgM-A

characteristic: title


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#1 IgM-B


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#1 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#1 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#1 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#1 IgM-F


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-A


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-B


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: WT#2 IgM-F


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #2 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #2 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #2 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #2 IgM-F


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #1 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #1 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #1 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 +/+ #1 IgM-F


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-A


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-B


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #2 IgM-F


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-A


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-B


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-C


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-D


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-E


text: UNIVERSITY HOSPITAL TUEBINGEN

text: public

text: splenocytes

text: house mouse

text: Mus musculus

text: Mus musculus

text: spleen

text: TCL1 Nfat2 -/- #1 IgM-F


text: Listeria monocytogenes

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: Listeria monocytogenes

characteristic: subject id


text: Enterococcus faecalis

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis


characteristic: ENA-FIRST-PUBLIC

text: Functional Genomics Genter Zurich

text: public

tag: Namespace:Functional Genomics Genter Zurich

characteristic: collection date

text: Mycobacterium basiliensis - description of a new species

characteristic: description

text: Switzerland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Bronchoalveolar lavage

characteristic: isolation source

text: Mycobacterium sp. DSM 104308

text: Mycobacterium sp. DSM 104308


CECT 8871

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UVEG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: CECT 8871

characteristic: Submitter Id

text: water

characteristic: environment (biome)

characteristic: environment (feature)

text: water collected from the junction between the ocean and a freshwater spring, Jeju Island

characteristic: environment (material)

text: South Korea

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Primorskyibacter insulae

text: Whole genome sequencing of Primorskyivacter insulae CECT 8871, type strain

characteristic: project name

text: Primorskyibacter insulae

text: Illumina Miseq

characteristic: sequencing method

text: CECT 8871

characteristic: strain

text: Primorskyibacter insulae CECT 8871

text: water

characteristic: water environmental package


text: Oryzias latipes

characteristic: ArrayExpress-SPECIES

text: Kiyosu panel

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: Japanese medaka

characteristic: common name

text: Oryzias latipes

characteristic: organism

text: Oryzias latipes

characteristic: subject id

characteristic: title


text: Homo sapiens

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: IP

text: public

text: house mouse

text: C57BL/6J mice

characteristic: lab host

text: Mus musculus

text: Mus musculus

text: cecal

characteristic: tissue type

text: 16S sequencing of mice cecal tissue


text: IP

text: public

text: house mouse

text: C57BL/6J mice

text: CONV

text: Mus musculus

text: Mus musculus

text: cecal

text: 16S sequencing of mice cecal tissue


text: IP

text: public

text: house mouse

text: C57BL/6J mice

text: abx

text: Mus musculus

text: Mus musculus

text: cecal

text: 16S sequencing of mice cecal tissue


text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


E-MTAB-6530:Sample 1

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-6530:Sample 1

tag: Namespace:Cell Biology and Biophysics Unit. European Molecular Biology Laboratory

text: ArrayExpress

characteristic: broker name

text: Protocols: Sperm was collected from a single adult Phallusia mammillata animal obtained from the Roscoff Marine Station (France). Sperm from a single P. mammillata individual was lysed in 20 ml spooling buffer (75 mM NaCl, 25 mM EDTA pH 8.0, 1% SDS, 0.2mg/ml Proteinase K) at 55 degrees overnight with gentle shaking. 5 ml of saturated NaCl solution were added and the solution was mixed by gentle shaking till homogeneous. 25 ml of isopropanol were added and the solution was mixed by gentle shaking till homogeneous. The stringy DNA precipitate was fished out and washed in 70% ethanol. After removing the 70% ethanol, the DNA pellet was air dried and resuspended in 10mM Tris pH 8 at 55 degrees with gentle shaking. A genomic DNA library was prepared using the NEBNext Ultra II DNA library preparation kit starting with 50 ng of sheared material following the manufacturer's instructions.

characteristic: description

text: Phallusia mammillata

text: Phallusia mammillata

text: sperm

characteristic: organism part

text: Phallusia mammillata

text: Sample 1


E-MTAB-6528:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cell Biology and Biophysics Unit. European Molecular Biology Laboratory

characteristic: INSDC center name

text: public

text: E-MTAB-6528:Sample 7

text: ArrayExpress

text: Protocols: Adult Phallusia mammillata animals were obtained from the Roscoff Marine Station (France). Staged embryos were obtained after in vitro fertilization of eggs. RNA was extracted from staged embryos using the MirVana kit (Ambion) following the manufacturer's instructions. Barcoded mRNA libraries were prepared using TruSeq RNA Sample Preparation (Illumina) following the manufacturer's instructions.

text: 16-cell St5b

characteristic: developmental stage

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 7


E-MTAB-6528:Sample 8

characteristic: INSDC first public

text: public

text: E-MTAB-6528:Sample 8

text: ArrayExpress

text: 16-cell St5b

text: Phallusia mammillata

characteristic: organism

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 8

characteristic: title


E-MTAB-6528:Sample 10

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6528:Sample 10

text: ArrayExpress

text: 32-cell St6b

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 10


E-MTAB-6528:Sample 9

text: public

text: E-MTAB-6528:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: 32-cell St6b

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 9


E-MTAB-6528:Sample 3

text: public

text: E-MTAB-6528:Sample 3

text: ArrayExpress

text: 4-cell St3

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 3


E-MTAB-6528:Sample 4

text: public

text: E-MTAB-6528:Sample 4

text: ArrayExpress

text: 4-cell St3

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 4


E-MTAB-6528:Sample 11

text: public

text: E-MTAB-6528:Sample 11

text: ArrayExpress

text: 64-cell St8

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 11


E-MTAB-6528:Sample 12

text: public

text: E-MTAB-6528:Sample 12

text: ArrayExpress

text: 64-cell St8

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 12


E-MTAB-6528:Sample 5

text: public

text: E-MTAB-6528:Sample 5

text: ArrayExpress

text: 8-cell St4

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 5


E-MTAB-6528:Sample 6

text: public

text: E-MTAB-6528:Sample 6

text: ArrayExpress

text: 8-cell St4

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 6


E-MTAB-6528:Sample 13

text: public

text: E-MTAB-6528:Sample 13

text: ArrayExpress

text: Early Gastrula St11

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 13


E-MTAB-6528:Sample 14

text: public

text: E-MTAB-6528:Sample 14

text: ArrayExpress

text: Early Gastrula St11

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 14


E-MTAB-6528:Sample 17

text: public

text: E-MTAB-6528:Sample 17

text: ArrayExpress

text: Early Neurula St14

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 17


E-MTAB-6528:Sample 18

text: public

text: E-MTAB-6528:Sample 18

text: ArrayExpress

text: Early Neurula St14

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 18


E-MTAB-6528:Sample 23

text: public

text: E-MTAB-6528:Sample 23

text: ArrayExpress

text: Early Tailbud I St19

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 23


E-MTAB-6528:Sample 24

text: public

text: E-MTAB-6528:Sample 24

text: ArrayExpress

text: Early Tailbud I St19

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 24


E-MTAB-6528:Sample 29

text: public

text: E-MTAB-6528:Sample 29

text: ArrayExpress

text: Hatched Larva St26

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 29


E-MTAB-6528:Sample 30

text: public

text: E-MTAB-6528:Sample 30

text: ArrayExpress

text: Hatching Larva St26

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 30


E-MTAB-6528:Sample 21

text: public

text: E-MTAB-6528:Sample 21

text: ArrayExpress

text: Initial Tailbud I St17

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 21


E-MTAB-6528:Sample 22

text: public

text: E-MTAB-6528:Sample 22

text: ArrayExpress

text: Initial Tailbud I St17

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 22


E-MTAB-6528:Sample 27

text: public

text: E-MTAB-6528:Sample 27

text: ArrayExpress

text: Late Tailbud II St24

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 27


E-MTAB-6528:Sample 28

text: public

text: E-MTAB-6528:Sample 28

text: ArrayExpress

text: Late Tailbud II St24

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 28


E-MTAB-6528:Sample 15

text: public

text: E-MTAB-6528:Sample 15

text: ArrayExpress

text: Mid Gastrula St12

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 15


E-MTAB-6528:Sample 16

text: public

text: E-MTAB-6528:Sample 16

text: ArrayExpress

text: Mid Gastrula St12

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 16


E-MTAB-6528:Sample 19

text: public

text: E-MTAB-6528:Sample 19

text: ArrayExpress

text: Mid Neurula St15

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 19


E-MTAB-6528:Sample 20

text: public

text: E-MTAB-6528:Sample 20

text: ArrayExpress

text: Mid Neurula St15

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 20


E-MTAB-6528:Sample 25

text: public

text: E-MTAB-6528:Sample 25

text: ArrayExpress

text: Mid Tailbud II St22

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 25


E-MTAB-6528:Sample 26

text: public

text: E-MTAB-6528:Sample 26

text: ArrayExpress

text: Mid Tailbud II St22

text: Phallusia mammillata

text: Phallusia mammillata

text: whole organism

text: Phallusia mammillata

text: Sample 26


E-MTAB-6528:Sample 1

text: public

text: E-MTAB-6528:Sample 1

text: ArrayExpress

text: unfertilized egg

text: Phallusia mammillata

text: Phallusia mammillata

text: germ cell

text: Phallusia mammillata

text: Sample 1


E-MTAB-6528:Sample 2

text: public

text: E-MTAB-6528:Sample 2

text: ArrayExpress

text: unfertilized egg

text: Phallusia mammillata

text: Phallusia mammillata

text: germ cell

text: Phallusia mammillata

text: Sample 2


text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

characteristic: subject id


characteristic: ENA-CHECKLIST

text: IBENS

text: public

characteristic: description

text: Licmophora abbreviata

text: Licmophora abbreviata


text: Klebsiella pneumoniae

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


E-MTAB-6539:Sample 1

characteristic: ENA-LAST-UPDATE

text: Tangshan People’s Hospital

text: public

text: E-MTAB-6539:Sample 1

tag: Namespace:Tangshan People’s Hospital

text: Asterand Bioscience, CS-08 (r03)

characteristic: biosource provider

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: mammary epithelial cell

characteristic: cell type

text: human

text: Protocols: Cells are fixed in formaldehyde (or some other crosslinking reagent), such that any proteins that are bound to or near DNA are covalently linked to the chromatin. The fixation reaction is then quenched with addition of glycine, and the cells are harvested and spun down in ice-cold PBS 1. Resuspend the cell pellet in 10 mL of cell lysis buffer for ChIP (cold) and incubate for 10 min on ice. 2. Pellet the cell nuclei at 1000 rpm in a benchtop centrifuge for 10 min at 4°C. Aspirate the supernatant carefully. 3.Resuspend the cell nuclei in 500 μL of MNase digestion buffer. Add MNase and incubate for 1 h on ice. Stop the digest by adding EDTA to a final concentration of 50 mM. The amount of MNase will need to be optimized according to final chromatin size in Step 8. A range of 50-300 U is a convenient starting point. 4. Resuspend the nuclear pellet in 1 mL of nuclei lysis buffer for ChIP supplemented with protease inhibitors and incubate for 10 min on ice. 5. Proceed with sonication. Ensure that the samples do not foam and are kept as cold as possible. Sonication conditions will need to be optimized. Typically, six 15-sec pulses followed by 45-sec rest periods at output 6.0 have been found to work. It is helpful to analyze each sample for proper sonication. For this analysis, reverse the cross-links and purify a small volume of chromatin as described below, and then analyze on an agarose gel. DNA should ideally be between 300 and 1000 bp in size. 6. Transfer chromatin to a 1.5-mL microcentrifuge tube and freeze at −80°C for storage. 7. Thaw the chromatin on ice. Centrifuge samples at high speed in a microcentrifuge for 15 min at 4°C to pellet the precipitated SDS. Transfer the supernatant to a fresh microcentrifuge tube. At this step, it is helpful to aliquot chromatin for storage at −80°C. 8. Measure the DNA concentration (A260) of the chromatin using a spectrophotometer. Use nuclei lysis buffer for ChIP devoid of SDS as a blank. Typically, the chromatin concentration should be >750 ng/μL. The A260/A280 ratio should be ~1.4-1.6. 9. Aliquot 100 μg of chromatin per antibody to be used into microcentrifuge tubes. It is important to have an irrelevant antibody such as α-glutathione S-transferase as a control. 10. Dilute the chromatin to a final volume of 300 μL with dilution buffer supplemented with protease inhibitors. 11. To preclear the chromatin, add 50 μL of protein A-agarose/salmon sperm DNA or protein G-agarose/salmon sperm DNA beads to the chromatin and rotate for 1-2 h at 4°C. Use large-orifice tips or cut off the end of regular micropipettor tips for transfer of beads. 12. Centrifuge the chromatin samples at 3000 rpm for 5 min at 4°C. Transfer the supernatants to fresh microcentrifuge tubes. 13. Add relevant antibodies to the chromatin samples and rotate overnight at 4°C. 14. Add 50 μL of protein A-agarose/salmon sperm DNA or protein G-agarose/salmon sperm DNA (depending on antibody) beads to the chromatin samples on ice. Rotate for 2 h at 4°C. Make sure bead slurry is completely resuspended before adding to samples. 15. Centrifuge the samples at 3000 rpm for 2 min at 4°C. 16. Collect 15 μL from the supernatant of the control antibody sample as a 5% input. Keep this sample on ice for later use. 17. Carefully aspirate the supernatants of all samples. Be careful not to aspirate any beads. Narrow gel-loading tips are useful for careful aspiration. 18. Add 1 mL of high-salt wash buffer for ChIP to all samples and rotate for 10 min at room temperature. 19. Centrifuge the samples at 3000 rpm for 2 min at room temperature. 20. Carefully aspirate the supernatants and add 1 mL of high-salt wash buffer for ChIP. Rotate for 10 min at room temperature. 21. Repeat Steps 22-23 twice for a total of four high-salt washes. 22. Aspirate supernatants and wash twice with TE as above. 23. Resuspend beads and input samples in 300 μL of elution buffer for ChIP supplemented with 1 μL of proteinase K (20 μg/μL), and incubate samples for 2 h at 55°C. Do not resuspend beads by pipetting. Instead, gently flick or vortex samples. 24. To reverse cross-links, incubate overnight at 65°C. For library preparation was performed by the BerryGenomics Company using Illumina HiSeq2500 V4 with 50-bp paired-end reads.

text: metaplastic breast carcinoma

characteristic: disease

text: JMJD6 overexpression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-6539:Sample 2

characteristic: External Id

text: Tangshan People’s Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6539:Sample 2

text: Asterand Bioscience, CS-08 (r03)

text: ArrayExpress

text: mammary epithelial cell

text: human

text: metaplastic breast carcinoma

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-6539:Sample 3

characteristic: ENA-CHECKLIST

text: Tangshan People’s Hospital

text: public

characteristic: SRA accession

text: E-MTAB-6539:Sample 3

characteristic: Submitter Id

text: Asterand Bioscience, CS-08 (r03)

text: ArrayExpress

text: mammary epithelial cell

text: human

characteristic: common name

characteristic: description

text: metaplastic breast carcinoma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 3


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:DGIMI, Univ. Montpellier, INRA, Montpellier, France

text: ArrayExpress

characteristic: broker name

text: fall armyworm

text: Protocols: Spodoptera frugiperda wild larvae were collected in Florida and Georgia between September, 18th and September, 25th 2015 in three different field locations. One sweet corn field in Citra (Marion County, Florida), one volunteer corn in Tifton, (Tift County, Georgia) and one pasture grass field in Jacksonville (Duval County, Florida). In corn fields, plants were cut and larvae collected in situ. In the pasture grass field, collections were made using a sweeping net. After confirming their identification as Spodoptera frugiperda according to LepIntercept (http://idtools.org/id/leps/lepintercept/frugiperda.html), larvae were placed in individual plastic cups with cut leaves (either corn or grass) as a food source and brought back in a cooler to the laboratory after a few hours of collection. Once in the laboratory, larvae were sorted according to stage. Stages were measured according to the chart in Supplementary Figure 18, where the width of the cephalic capsule should match the width of the line for each stage. This chart has been determined based on rearing conditions of lab strains in Montpellier and confirmed with a similar chart based on the rearing of lab strains in Gainesville, Florida. L4 larvae were sacrificed with a razor blade and immediately placed in a screw-cap 2ml tube containing 1ml of RNAlater (Sigma; R0901). Younger larvae were placed again in individual cups with fresh cut leaves and were grown in a 25°C incubator until they reached the L4 stage. The reciprocal transplant (RT) experiment consisted in controlled infestations of corn and rice plants with first instar larvae in 8 insect rearing cages (32.5 x 32.5 cm) covered by an insect-proof veil to prevent contaminations and escapes in the incubator (24°C, 16h:8h L:D cycle and 70% R. H.). The RT experiment was conducted in the same incubator for four modalities: 1) corn plants infested by sf-C (native condition); 2) rice plants infested by sf-C (alternative condition); 3) corn plants infested by sf-R (alternative condition); 4) rice plants infested by sf-R (native condition). We realized two replicates by modality. Each cage contained four corn or rice pots, which were changed before the 4th larval instar and each day after this instar until the pupation. We deposited in each cage 80 larvae hatched the morning of the experiment initiation (larvae obtained from several egg-masses provided from breeding). Two generations have been conducted on plants; during the first generation we measured life history traits (LHT) for each strain in native and alternative conditions and during the second generation, the larvae had been sampled at 4th larval instar for a RNA-Seq analyzes. The reciprocal transplant (RT) experiment consisted in controlled infestations of corn and rice plants with first instar larvae in 8 insect rearing cages (32.5 x 32.5 cm) covered by an insect-proof veil to prevent contaminations and escapes in the incubator (24°C, 16h:8h L:D cycle and 70% R. H.). The RT experiment was conducted in the same incubator for four modalities: 1) corn plants infested by sf-C (native condition); 2) rice plants infested by sf-C (alternative condition); 3) corn plants infested by sf-R (alternative condition); 4) rice plants infested by sf-R (native condition). We realized two replicates by modality. Each cage contained four corn or rice pots, which were changed before the 4th larval instar and each day after this instar until the pupation. We deposited in each cage 80 larvae hatched the morning of the experiment initiation (larvae obtained from several egg-masses provided from breeding). Two generations have been conducted on plants; during the first generation we measured life history traits (LHT) for each strain in native and alternative conditions and during the second generation, the larvae had been sampled at 4th larval instar for a RNA-Seq analyzes. We weighed the pools and crushed them in liquid nitrogen to obtain a fine powder, which was placed in TRIzol® Reagent (Invitrogen) and stored at -80°C. After collection of samples in all experimental set-ups, total RNA was extracted using a TRIzol® Reagent, according to the manufacturer’s RNA protocol. To remove contaminating DNA from RNA preparations, we used DNase from TURBO DNA-free™ Kit (Ambion). qRNA quantity and quality were assessed using a Bioanalyzer and 1 µl of total RNA from each sample of three larvae. The pool of three larvae, having a good quality and quantity, were pooled to obtain 6 samples corresponding to the 2 biologic replicates of the 4 experimental set-ups. Library preparation is done using Illumina's TruSeq Stranded mRNA Sample Preparation kit. It consists of 7 steps: 1. The selection of polyadenylated RNAs on magnetic magnesium oligo (dT); 2. Chemical fragmentation of the selected RNAs 3. The synthesis of the first strand of cDNA (random primers + SuperScript II) in the presence of acetoincin D in order to block the synthesis of the second strand; The synthesis of the second strand of cDNA by replacing the dTTP with dUTP in order to block the synthesis of the second strand during the PCR amplification step; Adding a base To these extremes 6. The ligation of adpatators to these ends.7. A PCR amplification step. This step also allows for an election since only the fragments bearing the two types of adapters (cf step 6) will be amplified. The validation of the libraries is performed qualitatively and quantitatively on an Agilent DNA 1000 chip as well as by qPCR (Applied Biosystems). 7500 with SYBR Green chemistry).

text: larval stage

characteristic: developmental stage

text: wild larvae collected on corn field

characteristic: environmental history

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: C-strain

characteristic: strain


characteristic: External Id

text: DGIMI, Univ. Montpellier, INRA, Montpellier, France

characteristic: INSDC center name

text: public

text: ArrayExpress

text: fall armyworm

text: larval stage

text: wild larvae collected on corn field

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: C-strain


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fall armyworm

text: larval stage

text: wild larvae collected on corn field

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: R-strain


text: public

characteristic: INSDC status

text: ArrayExpress

text: fall armyworm

text: larval stage

text: wild larvae collected on pasture grass

text: Spodoptera frugiperda

characteristic: organism

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: R-strain


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: fall armyworm

text: larval stage

text: corn

characteristic: diet

text: laboratory strain

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: sf-R - second generation

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fall armyworm

characteristic: common name

text: larval stage

text: rice

text: laboratory strain

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: sf-R - second generation


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: fall armyworm

characteristic: description

text: larval stage

text: corn

text: laboratory strain

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: sf-C


text: public

text: ArrayExpress

characteristic: broker name

text: fall armyworm

text: larval stage

characteristic: developmental stage

text: artificial diet

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