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text: Protocols: We first used fluorescence-activated cell sorting (FACS) to purify a homogeneous population of NSCs (D0) based on the expression of the cell-surface markers CD184+/CD271-/CD44-/CD24-/CD15+. To trace ASD and control neurons over time, we performed a series of retroviral lineage-tracing experiments to trace the progenies of dividing NSCs using a retroviral vector expressing a membrane-tagged enhanced green fluorescent protein (eGFP) (CAG::LckN-eGFP). As differentiating neurons express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of defined subpopulations of retrovirally labeled eGFP+/PSA-NCAM+ double-positive neurons after 2, 4, 7 and 14 (D2, 4, 7 and 14) days of differentiation. IPSCs were sorted based on the expression of SSEA-4 and TRA1-81 and maturing iPSC-iNs were collected at the indicated days after induction by sorting for eGFP (indicative for the Ngn2 transgene)- and PSA-NCAM-positive cells. Induced iPSC neurons (iPSC-iNs): The cell surface markers SSEA4+ and TRA1-81+ were used for FACS-based purification of iPSCs. For iPSC-iNs, the human cDNA sequence for Ngn2 and the sequence for eGFP were linked by a 2A sequence and cloned into the tet-inducible pLV-XTP backbone (Clonetech). IPSCs were transduced with lentiviral particles for pLV-X-UbC:TetOn (UtO) and pLV-X-Ngn2:2A:eGFP (N2AG) and were selected in the presence of puromycin (0.5 μg/ml, Sigma Aldrich) and G418 (100 μg/ml, Life technologies). For iPSC-iN conversion, iPSCs were transferred into PluriPro monolayer conditions (Cell Guidance Systems) and, 24 hrs after plating, were induced with doxycycline (2 μg/ml: Sigma Aldrich) for two days. For further maturation, iNs were switched to Neuron Maturation Media (NMM) based on DMEM:F12/Neurobasal (both Gibco) (1:1) as used for directed differentiation. NMM contains the following supplements: N2 supplement (1x, Gibco), B27 supplement (1x, Gibco), Laminin (1 μg/ml, Life Technologies) and doxycycline (2 μg/ml: Sigma Aldrich). Medium was changed every other day. Differentiating iPSC-iNs were purified by gating on eGFP+/PSA-NCAM+ neurons at 3, 7 and 14 days after conversion. NSCs and maturing neuron cultures as well as iPSCs and iPSC-iNs were detached using accutase (Innovative Cell Technologies) and then washed and stained with fluorophore-coupled antibodies (Extended Data Table 11) for 45 min at 4ºC in phosphate buffered saline (PBS). Cells were washed, suspended in PBS and filtered using a 40-μm cell strainer. The desired cell populations were sorted directly into Trizol-LS (Life Technologies). RNA was isolated according to the manufacturer’s instructions and subsequently digested with TURBO DNase to remove trace quantities of DNA (ambion, Life Technologies). Before RNA-seq library preparation, assessment of RNA integrity numbers (RIN) was performed using the Agilent TapeStation. Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit according to the manufacturer’s instructions (Illumina).

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text: Protocols: We first used fluorescence-activated cell sorting (FACS) to purify a homogeneous population of NSCs (D0) based on the expression of the cell-surface markers CD184+/CD271-/CD44-/CD24-/CD15+. To trace ASD and control neurons over time, we performed a series of retroviral lineage-tracing experiments to trace the progenies of dividing NSCs using a retroviral vector expressing a membrane-tagged enhanced green fluorescent protein (eGFP) (CAG::LckN-eGFP). As differentiating neurons express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of defined subpopulations of retrovirally labeled eGFP+/PSA-NCAM+ double-positive neurons after 2, 4, 7 and 14 (D2, 4, 7 and 14) days of differentiation. IPSCs were sorted based on the expression of SSEA-4 and TRA1-81 and maturing iPSC-iNs were collected at the indicated days after induction by sorting for eGFP (indicative for the Ngn2 transgene)- and PSA-NCAM-positive cells. NSC-derived neurons: We differentiated iPSCs into NSCs by following a default differentiation protocol. Briefly, iPSC colonies were detached by collagenase (Gibco) to form embryoid bodies (EBs) and then cultured in suspension in the presence of 1 μM dorsomorphin (Cayman Chemical). After floating for seven days, EBs were attached on poly-ornithine (10 μg/ml, Life Technologies) and laminin (5 μg/ml, Life Technologies) coated surfaces. At day 16, emerging rosette colonies were manually detached, gently dissociated with accutase (Innovative Cell Technologies) and plated as NSC monolayers onto coated dishes for further neuronal differentiation. We first used fluorescence-activated cell sorting (FACS) to purify a homogeneous population of NSCs based on the expression of the cell-surface markers CD184+/CD271-/CD44-/CD24-/CD15+. To trace ASD and control neurons over time, we performed a series of retroviral lineage-tracing experiments to trace the progenies of dividing NSCs using a retroviral vector expressing a membrane-tagged enhanced green fluorescent protein (eGFP) (CAG::LckN-eGFP). Briefly, the sequence of the membrane localization domain from Lck (LckN) was linked to the eGFP sequence and cloned into the Moloney Murine Leukemia Virus (MMLV)-based retroviral plasmid resulting in pRV-CAG:LckN-eGFP (CAG:LckN-eGFP). As differentiating neurons express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of defined subpopulations of retrovirally labeled eGFP+/PSA-NCAM+ double-positive neurons after 2, 4, 7 and 14 days of differentiation. NSCs and maturing neuron cultures as well as iPSCs and iPSC-iNs were detached using accutase (Innovative Cell Technologies) and then washed and stained with fluorophore-coupled antibodies (Extended Data Table 11) for 45 min at 4ºC in phosphate buffered saline (PBS). Cells were washed, suspended in PBS and filtered using a 40-μm cell strainer. The desired cell populations were sorted directly into Trizol-LS (Life Technologies). RNA was isolated according to the manufacturer’s instructions and subsequently digested with TURBO DNase to remove trace quantities of DNA (ambion, Life Technologies). Before RNA-seq library preparation, assessment of RNA integrity numbers (RIN) was performed using the Agilent TapeStation. Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit according to the manufacturer’s instructions (Illumina).

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text: Gut dysbiosis in patients with multiple sclerosis is characterized by bacteria that regulate T lymphocyte differentiation in vitro

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characteristic: INSDC status

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characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 0376.1.q

characteristic: description

text: RRMS

characteristic: disease course

text: MS

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: No

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: Missing: Not provided

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Off

text: Missing: Not provided

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 0402.1.Q.1133346

text: RRMS

text: MS

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: No

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

characteristic: treatment status

text: Off

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 0415.2.q

characteristic: description

text: RRMS

characteristic: disease course

text: MS

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: No

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Off

text: Missing: Not provided

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 0599.1.q

text: RRMS

text: MS

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: No

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

characteristic: treatment status

text: Naive

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 0601.1.q

characteristic: description

text: RRMS

characteristic: disease course

text: MS

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: No

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Naive

text: Missing: Not provided

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Missing: Not provided

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1003.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: Missing: Not provided

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1004.1.Q.1133418

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1008.1.Q.1133343

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1010.1.Q.1133355

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1012.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1018.1.Q.1133334

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1025.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1027.1.Q.1133330

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Missing: Not provided

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1033.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1034.1.q

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1038.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1048.1.q

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1053.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1054.1.q

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Missing: Not provided

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 1058.1.q

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: male

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 1059.1.q

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 2017.1.Q.1133337

text: RRMS

text: MS

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: No

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

characteristic: treatment status

text: Naive

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 2026.1.q

characteristic: description

text: RRMS

characteristic: disease course

text: MS

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: No

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: Missing: Not provided

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Naive

text: Missing: Not provided

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 2027.1.q

text: RRMS

text: MS

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: No

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

characteristic: treatment status

text: Naive

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 2028.1.q

characteristic: description

text: RRMS

characteristic: disease course

text: MS

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: No

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: Missing: Not provided

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Naive

text: Missing: Not provided

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: UCSF

text: Missing: Not provided

text: fecal sample 2037.1.q

text: RRMS

text: MS

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: No

text: USA:CA:San Francisco

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: UCSF

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

characteristic: treatment status

text: Naive

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: UCSF

characteristic: collection site

text: Missing: Not provided

text: fecal sample 3000.1.Q.1133420

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:CA:San Francisco

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: Missing: Not provided

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: UCSF

text: FALSE

text: stool

text: human gut metagenome

text: male

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Sinai

text: Missing: Not provided

text: fecal sample C.PC10AF.1133359

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:NY:New York

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: Sinai

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Sinai

characteristic: collection site

text: Missing: Not provided

text: fecal sample C.PC15AF.1133328

characteristic: description

text: Control

characteristic: disease course

text: Control

characteristic: disease state

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Control

characteristic: flare

text: USA:NY:New York

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: human

text: Homo sapiens

text: Missing: Not provided

text: mimarks-survey

text: adult

text: human gut metagenome

characteristic: organism

text: Sinai

text: FALSE

text: stool

text: human gut metagenome

text: female

text: Missing: Not provided

text: No

text: Control

text: Control

text: Missing: Not provided

text: Missing: Not provided

text: dry

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: years

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Sinai

text: Missing: Not provided

text: fecal sample C.PC16AF.1133415

text: Control

text: Control

text: TRUE

text: urban biome

text: human-associated habitat

text: human-gut

text: feces

text: Control

text: USA:NY:New York

text: Missing: Not provided

text: Missing: Not provided

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: Missing: Not provided

characteristic: household

text: mimarks-survey

characteristic: investigation type

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: human gut metagenome

text: Sinai

text: FALSE

text: stool

characteristic: sample type

text: human gut metagenome

text: female

characteristic: sex

text: Missing: Not provided

text: No

characteristic: timeseries

characteristic: title

text: Control

characteristic: treatment status

text: Control

characteristic: treatment type

text: Missing: Not provided

characteristic: weight

text: Missing: Not provided

text: dry

text: Control