text: Institute of Molecular Biology (IMB)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: The 6-well tissue culture plate was put on ice and media was removed from each well. Next, each well was washed with 1 ml of ice-cold PBS. Cells were then harvested in 1 ml of ice-cold PBS using a cell scraper and next collected by centrifugation at 500xg for 5 min at 4°C. The supernatant was subsequently removed and the cell pellet was either snap-frozen and stored or immeadiately subjected to nucleic acid extraction. MCF7 cells were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum at 37°C with 5% CO2. One well of a 6-well tissue culture with 90% confluent cells was transfected with 2 µg of a minigene library one day prior to harvesting using 20 µg polyethylenimine MW ~ 2500 transfection reagent (Polysciences, Inc.). The RON minigene was cloned into the pcDNA 3.1 (+) vector (Invitrogen). The minigene construct contains a sequence of the MST1R gene including exon 11 together with the complete flanking introns and the constitutive exons 10 and 12 (chr3: 49,933,098 - 49,933,837, GRCh37/hg19). The RON minigene library comprises of approximately 6,000 minigenes with three to four mutations on average per minigene. RNA extraction was performed using the QIAGEN RNeasy plus kit according to the manufacturer’s instructions. 17 µg of total RNA was used for poly(A)-selection using magnetic Dynabeads® Oligo (dT)25 from Thermo Fisher Scientific according to the manufacturer's instructions. 400 - 500 ng of the resulting poly(A) selected RNA was used for cDNA synthesis using the RevertAid reverse transcriptase from Thermo Fisher Scientific according to the manufacturer's instructions. The resulting cDNA was used as a template for PCR amplification via an emulsion PCR protocol as described by Williams et al., 2006. The following steps were then performed to clean the water-in-oil emulsion: First, the water phase was extracted by washing twice with 1 ml of water-saturated diethyl ether. Second, residual solvent from the broken emulsion was removed by centrifugation under vacuum for 12 min at 30°C.Finally, PCR products were purified using 2 volumes of Agencourt® AMPure® XP beads from Beckman Coulter according to the maufacturer's instructions.

characteristic: description

text: invasive ductal carcinoma

characteristic: disease

text: pool of minigene library MST1R construct

characteristic: genotype

text: pleural effusion

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Molecular Biology (IMB)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Institute of Molecular Biology (IMB)

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

text: Protocols: The 6-well tissue culture plate was put on ice and media was removed from each well. Next, each well was washed with 1 ml of ice-cold PBS. Cells were then harvested in 1 ml of ice-cold PBS using a cell scraper and next collected by centrifugation at 500xg for 5 min at 4°C. The supernatant was subsequently removed and the cell pellet was either snap-frozen and stored or immeadiately subjected to nucleic acid extraction. MCF7 cells were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum at 37°C with 5% CO2. One well of a 6-well tissue culture with 90% confluent cells was transfected with 2 µg of a minigene library one day prior to harvesting using 20 µg polyethylenimine MW ~ 2500 transfection reagent (Polysciences, Inc.). The RON minigene was cloned into the pcDNA 3.1 (+) vector (Invitrogen). The minigene construct contains a sequence of the MST1R gene including exon 11 together with the complete flanking introns and the constitutive exons 10 and 12 (chr3: 49,933,098 - 49,933,837, GRCh37/hg19). The RON minigene library comprises of approximately 6,000 minigenes with three to four mutations on average per minigene. Knockdown of HNRNPH was carried out by treating 20% confluent cells with siRNA against HNRNPH (5′-GGAGCUGGCUUUGAGAGGA[dT][dT], Sigma Aldrich) at a final concentration of 20 nM two days prior to harvesting. RNAiMax from Invitrogen was used for transfection according to the manufacturer's instructions. RNA extraction was performed using the QIAGEN RNeasy plus kit according to the manufacturer’s instructions. 17 µg of total RNA was used for poly(A)-selection using magnetic Dynabeads® Oligo (dT)25 from Thermo Fisher Scientific according to the manufacturer's instructions. 400 - 500 ng of the resulting poly(A) selected RNA was used for cDNA synthesis using the RevertAid reverse transcriptase from Thermo Fisher Scientific according to the manufacturer's instructions. The resulting cDNA was used as a template for PCR amplification via an emulsion PCR protocol as described by Williams et al., 2006. The following steps were then performed to clean the water-in-oil emulsion: First, the water phase was extracted by washing twice with 1 ml of water-saturated diethyl ether. Second, residual solvent from the broken emulsion was removed by centrifugation under vacuum for 12 min at 30°C.Finally, PCR products were purified using 2 volumes of Agencourt® AMPure® XP beads from Beckman Coulter according to the maufacturer's instructions.

text: invasive ductal carcinoma

text: pleural effusion

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


text: 34414415; 34461978; 35780211

text: FZB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DEU

characteristic: SRA accession

text: Mycobacterium tuberculosis complex bacterium

text: Mycobacterium

characteristic: genus

characteristic: isolate

text: WGS of MTBC isolates

type: AMR curated data - CABBAGE

value: pyrazinamide

value: susceptible

value: Mycobacterium tuberculosis

value: ethambutol

value: isoniazid

value: rifampin


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates

value: streptomycin


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates

value: resistant


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211; 35260883

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates

value: pretomanid

value: broth dilution

value: mg/L


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates

value: moxifloxacin

value: ofloxacin


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33239092; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: Homo sapiens

characteristic: host

text: WGS of MTBC isolates

value: clofazimine

value: bedaquiline


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates

value: kanamycin


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211; 35260883

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SRB

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33239092; 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: Homo sapiens

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211; 35260883

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33239092; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: Homo sapiens

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SRB

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 33347430; 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SRB

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SRB

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SWZ

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: SRB

text: Mycobacterium

text: WGS of MTBC isolates


text: 34414415; 34461978; 35780211

text: FZB

text: public

text: DEU

text: Mycobacterium

text: WGS of MTBC isolates


text: Institute of Molecular Biology (IMB)

text: public

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: The RON minigene was cloned into the pcDNA 3.1 (+) vector (Invitrogen). The minigene construct contains a sequence of the MST1R gene including exon 11 together with the complete flanking introns and the constitutive exons 10 and 12 (chr3: 49,933,098 - 49,933,837, GRCh37/hg19). The RON minigene library comprises of approximately 6,000 minigenes with three to four mutations on average per minigene. E. coli was transformed with a pool of plasmids generated by cloning of a mutated minigene library. Approximately 6,000 of the resulting E. coli transformants were harvested in LB-media. Next, the containing plasmids were extracted using the QIAGEN Plasmid Midi Kit according to the manufacturer's instructions. DNA was extracted from E. coli using the QIAGEN Plasmid Midi Kit according to the manufacturer's instructions. 8.25 ng of plasmid DNA was used as a template for PCR amplification of three amplicons via an emulsion PCR protocol as described by Williams et al., 2006. The same reverse primer was used for each amplicon (5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT-TCCGATCTNNNNNNNNNNATAGAATAGGGCCCTCTAGA 3') but different forward primer (in 5'-3' direction):1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG-CTCTTCCGATCTNNNNNNNNNNCTATAGGGAGACCCAAGCTT 2 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG-CTCTTCCGATCTNNNNNNNNNNGTTCCACTGAAGCCTGAG3 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG-CTCTTCCGATCTNNNNNNNNNNAGCTGCCAGCACGAGTTCFollowing the PCR amplification, the following steps were performed to clean the water-in-oil emulsion: First, the water phase was extracted by washing twice with 1 ml of water-saturated diethyl ether. Second, residual solvent from the broken emulsion was removed by centrifugation under vacuum for 12 min at 30°C.Finally, PCR products were purified using the Gene Read Size selection kit from QIAGEN according to the maufacturer's instructions.

characteristic: description

text: plasmid pool of microgene library containing MST1R constructs

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: amplicon pool 1

characteristic: plasmid

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Molecular Biology (IMB)

text: public

text: ArrayExpress

text: human

text: Protocols: The RON minigene was cloned into the pcDNA 3.1 (+) vector (Invitrogen). The minigene construct contains a sequence of the MST1R gene including exon 11 together with the complete flanking introns and the constitutive exons 10 and 12 (chr3: 49,933,098 - 49,933,837, GRCh37/hg19). The RON minigene library comprises of approximately 6,000 minigenes with three to four mutations on average per minigene. E. coli was transformed with a pool of plasmids generated by cloning of a mutated minigene library. Approximately 6,000 of the resulting E. coli transformants were harvested in LB-media. Next, the containing plasmids were extracted using the QIAGEN Plasmid Midi Kit according to the manufacturer's instructions. DNA was extracted from E. coli using the QIAGEN Plasmid Midi Kit according to the manufacturer's instructions. 8.25 ng of plasmid DNA was used as a template for PCR amplification of two amplicons via an emulsion PCR protocol as described by Williams et al., 2006. The same reverse primer was used for each amplicon (5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT-TCCGATCTNNNNNNNNNNATAGAATAGGGCCCTCTAGA 3') but different forward primer (in 5'-3' direction):1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG-CTCTTCCGATCTNNNNNNNNNNGAATCTGAGTGCCCGAGG 2 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG-CTCTTCCGATCTNNNNNNNNNNctactggctggtcctcatga Following the PCR amplification, the following steps were performed to clean the water-in-oil emulsion: First, the water phase was extracted by washing twice with 1 ml of water-saturated diethyl ether. Second, residual solvent from the broken emulsion was removed by centrifugation under vacuum for 12 min at 30°C.Finally, PCR products were purified using the Gene Read Size selection kit from QIAGEN according to the maufacturer's instructions.

text: Homo sapiens

text: Homo sapiens

text: amplicon pool 2

text: Homo sapiens


E-MTAB-6230:DDX17 Rep1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6230:DDX17 Rep1

tag: Namespace:Gurdon Institute, University of Cambridge

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: MCF7 cells were cultured in 10 cm diameter dishes. When cells reached confluence, media was removed and cells were washed twice with ice-cold PBS 1X. 6ml of ice cold PBS 1X were added, plates were placed on ice and irradiated once at 1500mJ/cm2 254 nm. Cell lysis, immunoprecipitation, 3’ end adapter ligation, 5’ end radioactive labelling were performed as described in Huppertz, I., et al., iCLIP: protein-RNA interactions at nucleotide resolution. Methods, 2014. protein-bound RNA isolation were performed as described in Huppertz, I., et al., iCLIP: protein-RNA interactions at nucleotide resolution. Methods, 2014. iCLIP library preparation was performed as described in Huppertz, I., et al., iCLIP: protein-RNA interactions at nucleotide resolution. Methods, 2014. The following Retro Transcription oligos (RToligos) were used to insert differential barcodes for each sample: Rt8clip (DDX3X rep1) /5Phos/NNCATTNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC Rt9clip (DDX3X rep2) /5Phos/NNGCCANNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC Rt11clip(DDX54rep1) 5Phos/NNGGTTNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC Rt13clip(DDX54rep2) /5Phos/NNTCCGNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC Rt6clip (Rb IgG) /5Phos/NNCCGGNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC Rt10clip(MsIgG) /5Phos/NNGACCNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC RToligos contain sequences for P5/P7 Illumina adapters for library PCR amplification and following high-throuput sequencing. A sample-specific adapter sequence is located immediately following the P7 adapter sequence. These barcodes are 9-mers of the format NNNXXXXNN, where XXXX was a fixed 4 bp barcode for each sample that allow de-multiplexing, and the Ns were randomly incorporated bases that allow PCR duplicates removal. iCLIP library preparation was performed as described in Huppertz, I., et al., iCLIP: protein-RNA interactions at nucleotide resolution. Methods, 2014.

text: invasive ductal carcinoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: DDX17 Rep1


E-MTAB-6230:DDX17 Rep2

text: Gurdon Institute, University of Cambridge

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6230:DDX17 Rep2

text: ArrayExpress

text: epithelial cell

text: human

text: invasive ductal carcinoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DDX17 Rep2

characteristic: title


E-MTAB-6230:DDX3 Rep1

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-6230:DDX3 Rep1

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: description

text: invasive ductal carcinoma

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DDX3 Rep1


E-MTAB-6230:DDX3 Rep2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6230:DDX3 Rep2

text: ArrayExpress

text: epithelial cell

text: human

text: invasive ductal carcinoma

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DDX3 Rep2


E-MTAB-6230:DDX54 Rep1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6230:DDX54 Rep1

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: invasive ductal carcinoma

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DDX54 Rep1


E-MTAB-6230:DDX54 Rep2

text: public

text: E-MTAB-6230:DDX54 Rep2

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: invasive ductal carcinoma

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DDX54 Rep2


E-MTAB-6230:Mouse IgG

characteristic: INSDC last update

text: public

text: E-MTAB-6230:Mouse IgG

text: ArrayExpress

text: epithelial cell

text: human

text: invasive ductal carcinoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Mouse IgG


E-MTAB-6230:Rabbit IgG

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6230:Rabbit IgG

text: ArrayExpress

text: epithelial cell

text: human

text: invasive ductal carcinoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Rabbit IgG


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: Protocols: MCF7 cells transfected with either scramble siRNA or DDX54 targeting siRNAs (#9 or 12), or DDX3X targeting siRNA (#6 or #8) harvested 72 h after transfection. Total RNA was purified from cells using miRNeasy Mini Kit (Qiagen) following manufacturer instructions. Excess of contaminating DNA was removed by On-Column DNase Digestion with the RNase-Free DNase Set (Qiagen), following manufacturer’s instructions. Ribosomal RNA was depleted with Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) from Illumina, following manufacturer’s instructions. All conditions were produced in three independent biological replicates. RNA seq libraries were produced with NEXTflex RNA-Seq Kit for Illumina Sequencing from Bioo Scientific, following manufacturer’s instructions. The following Illumina barcodes were used. Sample Sequence TruSeq code Scrambled REP 1 CGATGT A002 DDX3 siRNA #6 REP1 TGACCA A004 DDX3 siRNA #8 REP1 ACAGTG A005 DDX54 siRNA #9 REP1 GCCAAT A006 DDX54 siRNA #12 REP1 CAGATC A007 Scrambled REP 2 CTTGTA A012 DDX3 siRNA #6 REP 2 ATCACG A001 DDX3 siRNA #8 REP 2 TTAGGC A003 DDX54 siRNA #9 REP 2 ACTTGA A008 DDX54 siRNA #12 REP 2 GATCAG A009 Scrambled REP 3 TAGCTT A010 DDX3 siRNA #6 REP 3 GGCTAC A011 DDX3 siRNA #8 REP 3 AGTCAA A013 DDX54 siRNA #9 REP 3 AGTTCC A014 DDX54 siRNA #12 REP 3 ATGTCA A015 RNA seq libraries were produced with NEXTflex RNA-Seq Kit for Illumina Sequencing from Bioo Scientific, following manufacturer’s instructions.

text: invasive ductal carcinoma

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: PRIF UK

text: public

tag: Namespace:PRIF UK

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: CMP


name: Paired-end reads for sewage samples from Utrecht, NL

characteristic: External Id

text: UMC Utrecht

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:UMC Utrecht

characteristic: collection date

text: Sequence reads for sewage metagenomic samples

text: Netherlands

characteristic: geographic location (country and/or sea)

text: metagenome

characteristic: investigation type

text: metagenomes

text: metagenomes

text: wastewater treatment plant

characteristic: sewage type

text: Sewage samples


a nirk

text: Carvalho

characteristic: INSDC last update

text: public

text: a nirk

characteristic: Submitter Id

text: sludge

characteristic: environment (biome)

text: bioreactor

characteristic: environment (feature)

text: wastewater

characteristic: environment (material)

text: Portugal

text: 38.8 N

unit: DD

characteristic: geographic location (latitude)

text: 9.2 W

characteristic: geographic location (longitude)

text: metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

characteristic: project name

text: bioreactor sludge metagenome

text: pyrosequencing

characteristic: sequencing method

text: nirs nirK nosZ batch tests transcripts

text: wastewater/sludge

characteristic: wastewater/sludge environmental package

type: has member


b nirk

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Carvalho

text: public

characteristic: INSDC status

characteristic: SRA accession

text: b nirk

text: sludge

text: bioreactor

text: wastewater

text: Portugal

text: 38.8 N

text: 9.2 W

text: metagenome

text: bioreactor sludge metagenome

characteristic: organism

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: pyrosequencing

text: nirs nirK nosZ batch tests transcripts

characteristic: title

text: wastewater/sludge


c nirk

text: Carvalho

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: c nirk

characteristic: Submitter Id

characteristic: collection date

text: sludge

text: bioreactor

text: wastewater

text: Portugal

characteristic: geographic location (country and/or sea)

text: 38.8 N

text: 9.2 W

text: metagenome

characteristic: investigation type

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: pyrosequencing

text: nirs nirK nosZ batch tests transcripts

text: wastewater/sludge


d nirk

text: Carvalho

text: public

text: d nirk

text: sludge

characteristic: environment (biome)

text: bioreactor

characteristic: environment (feature)

text: wastewater

characteristic: environment (material)

text: Portugal

text: 38.8 N

characteristic: geographic location (latitude)

text: 9.2 W

characteristic: geographic location (longitude)

text: metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

characteristic: project name

text: bioreactor sludge metagenome

text: pyrosequencing

characteristic: sequencing method

text: nirs nirK nosZ batch tests transcripts

text: wastewater/sludge


e nirk

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Carvalho

text: public

characteristic: INSDC status

characteristic: SRA accession

text: e nirk

text: sludge

text: bioreactor

text: wastewater

text: Portugal

text: 38.8 N

text: 9.2 W

text: metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: pyrosequencing

text: nirs nirK nosZ batch tests transcripts

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


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text: Carvalho

characteristic: INSDC center name

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text: 38.8 N

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a nirs

characteristic: ENA-CHECKLIST

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text: sludge

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c nirs

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f nirs

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characteristic: collection date

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text: bioreactor

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text: Portugal

characteristic: geographic location (country and/or sea)

text: 38.8 N

characteristic: geographic location (latitude)

text: 9.2 W

text: metagenome

text: bioreactor sludge metagenome

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characteristic: ENA-CHECKLIST

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characteristic: organism

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a nosz

text: Carvalho

characteristic: INSDC center name

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text: public

characteristic: SRA accession

text: a nosz

characteristic: Submitter Id

text: sludge

text: bioreactor

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text: 38.8 N

text: 9.2 W

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

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b nosz

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characteristic: collection date

text: sludge

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text: bioreactor

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text: wastewater

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text: 38.8 N

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text: metagenome

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text: 9.2 W

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text: bioreactor

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text: Portugal

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text: 38.8 N

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text: metagenome

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f nosz

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text: sludge

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text: sludge

text: bioreactor

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text: 38.8 N

characteristic: geographic location (latitude)

text: 9.2 W

text: metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: bioreactor sludge metagenome

text: pyrosequencing

text: nirs nirK nosZ batch tests transcripts

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


text: public

tag: Namespace:FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

characteristic: collection date

text: Salmonella enterica subsp. enterica serovar Enteritidis

characteristic: common name

text: Germany

characteristic: geographic location (country and/or sea)

text: Salmonella enterica

characteristic: isolate

text: animal

characteristic: isolation source

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text: Enteritidis

characteristic: serovar

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text: ENGAGE-project Salmonella strains from BfR

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characteristic: External Id

text: FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: public

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text: Germany

text: Salmonella enterica

text: animal

text: Enteritidis

text: enterica


characteristic: INSDC center name

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text: public

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text: Germany

text: Salmonella enterica

text: food

text: Enteritidis

text: enterica


text: Instituto Aggeu Magalhaes

text: public

tag: Namespace:Instituto Aggeu Magalhaes

text: Chikungunya virus

text: Chikungunya virus

text: Chikungunya virus ECSA genotype from Feira de Santana


text: Michigan State University

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tag: Namespace:Michigan State University

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text: Pond: Tiedeman South

characteristic: description

text: tundra

characteristic: environment (biome)

text: freshwater pond

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text: biofilm

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text: High Nitrate

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text: USA

characteristic: geographic location (country and/or sea)

unit: m

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text: mimarks-survey

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text: Copper River Delta - Impact of Nutrient Limitation

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text: High Nitrate

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text: USA

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text: Michigan State University

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text: Pond: Storey North

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text: biofilm

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