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Control_Rep_1_port_3_day 96

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text: Mycobacterium simulans strain FB-527 was isolated from the sputum of a Djiboutian patient who had been diagnosed with cavitary tuberculosis

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text: Protocols: Flies expressing EGFP-CBP20 under control of a mesodermal enhancer were grown in population cages at 25 degrees. After three/four 1 hr pre-lays, the flies were allowed to lay for 1 or 2 hrs, after which the embryos were aged at the same temperature to the appropriate time-point. The embryos were then dochorionated using 50% bleach, washed with distillate water and PBT (PBS + 1% Triton X-100), and directly subjected to homogenization on ice. All steps were done at 4C. 500 ul of embryo suspension was added to 6.5 ml Schneider???s media with Actinomycin D in a 15 ml dounce homogenizer on ice and dounced with a loose pestle 7 times, in aliquots of less than 0.0625 g of embryos. Material from two douncing rounds was combined in one 15 ml tube and centrifuged at 600 rpm for 5 min at 4C. Supernatant was transferred into a clean tube and centrifuged at 1700 rpm for 10 min at 4C. Supernatant was discarded, and 250 ul of Schneider???s media complemented with 8% fetal bovine serum and 1 ug/ml of Actinomycin D was added to the pellet. All resuspended pellets were combined into a single tube, cells were gently passed through a 18-gauge needle 5 times and sieved through a 40 um sieve into a 50 ml tube. Approximately 5% of the total sample was transferred into a RNAse free 1.5ml tube and centrifuged at 800 g for 10 min at 4C. 600 ul TRIzol was added to the pellet and saved as unsorted sample. Remaining sample was used for fluorescence activated cell sorting (FACS). Cell sorter (MoFlo, Beckman Coulter) was precooled at 4C and run with 70 um nozzle and 5,000 ??? 10,000 cells per second. Sorted cells were collected in 5 ml round bottom polypropylene tubes in 500 ul Seecof saline (6 mM Na2HPO4, 3.67 mM KH2PO4, 106 mM NaCl, 26.8 mM KCl, 6.4 mM MgCl2, 2.25 mM CaCl2, pH 6.8) supplemented with 0.1 U/ul RNAse inhibitor. Sorted cells were aliquoted in low binding RNAse free tubes and centrifuged at 800 g 10 min at 4C. Pellet was resuspended in 150 ul TRIzol LS and all samples were pooled into a single tube before proceeding with RNA isolation. RNA isolation was done according to manufacturer???s instructions for TRIzol LS, including overnight precipitation step with 1 ul glycogen at -80C. RNA was treated with RNAse free DNAse I (Roche) for 30 min and purified with Agencourt RNAclean XP beads (Beckam Coulter) according to manufacturer???s instructions. 'U' extracts: RNA extracted from cells before sorting (see below) 'S' extracts: RNA extracted from sorted cells. Production of libraries containing 27bp-tags from 5' ends of capped transcripts. Taken from Takahashi et al, Nat Prot 2012. Briefly, 1-5ug of total RNA are retrotranscribed with a primer containing a common sequence followed by 15 Ns. The RNA-cDNA duplexed is subjected to in-vitro biotinylation of the 5'cap. Then the single-stranded RNA is degraded with RNaseI and the remaining biotinylated (capped) RNA-cDNA duplexes are pull-down with streptavidin-conjugated Dynabeads. cDNA is released through NaOH treatment, and then a barcoded 5'adapter is ligated to the 3'end of the single strand cDNA. Non-ligated adapter is removed by purifying twice with AMPure XP beads. Second strand is synthesized using a primer corresponding to the 5'adapter. Then, 27bp-long tags are generated by digestion with EcoP15I, a type III restriction enzyme. The two matched restriction sites are in the 5'adapter and in the RT primer sequences. A common 3'adapter is then ligated to the resulting fragments. The libraries are amplified by PCR, tipically for 8-15 cycles. After PCR, primers are removed with exonuclease treatment, and the double strand fragments are purified both with QIAquick columns and AMPure XP beads to remove unwanted adapter dimers.

text: stage 10-11

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text: Drosophila melanogaster

text: Drosophila melanogaster

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text: Drosophila melanogaster


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tag: Namespace:COPENHAGEN UNIVERSITY

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text: MS RRMS

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text: feces

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: stool

characteristic: host body product

text: MS RRMS

characteristic: host disease status

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

characteristic: organism

text: Forward: 5'-AGAGTTTGATCCTGGCTCAG-3' Reverse: 5'-ATTACCGCGGCTGCTGG-3'

characteristic: pcr primers

text: Microbiota composition of MS and PD patients

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text: Homo sapiens

text: Illumina Miseq

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text: 16S rRNA

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text: Stool sample - 16S amplicon sequencing

characteristic: title


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text: COPENHAGEN UNIVERSITY

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text: Germany

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text: Homo sapiens

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text: Stool sample - 16S amplicon sequencing


text: COPENHAGEN UNIVERSITY

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text: excreta

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text: feces

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text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

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text: stool

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text: MS SPMS

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text: human-gut

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text: metagenome

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text: Homo sapiens

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text: 16S rRNA

characteristic: target gene

text: Stool sample - 16S amplicon sequencing


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text: public

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text: Stool sample - 16S amplicon sequencing

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text: COPENHAGEN UNIVERSITY

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text: feces

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text: Germany

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text: Homo sapiens

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text: ETH ZURICH

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tag: Namespace:ETH ZURICH

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text: Population Bourges (industrial area), maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 03 , halfsib a

characteristic: description

text: Dittrichia graveolens

text: Dittrichia graveolens

characteristic: title


text: ETH ZURICH

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text: Population Bourges (industrial area), maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (industrial area), maternal family 16 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 01 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 16 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 18 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Bourges (highway), maternal family 19 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 16 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cournon-d'Auvergne, maternal family 17 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib d

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib e

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 03 , halfsib f

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Cuijk, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 11 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 13 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 14 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 14 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 14 , halfsib d

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Fr�jus, maternal family 14 , halfsib e

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 02 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 02 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 02 , halfsib d

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population 't Harde, maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (highway), maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 01 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 01 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 03 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 03 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Issoire (industrial area), maternal family 17 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 01 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 02 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 03 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 04 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Laren, maternal family 05 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 17 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 18 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 19 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 20 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 21 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 22 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Gare de Lonay-Pr�verenges, maternal family 23 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 07 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 08 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Aire du Merle nord, maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (roadside), maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 18 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montlu�on (field), maternal family 19 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 19 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 21 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Montmarault, maternal family 22 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 01 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 14 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population M�nsingen highway exit, maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 13 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Chemin d'Ogoz, Saint-Saphorin, maternal family 15 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 05 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 07 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 11 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 11 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population P�gomas, maternal family 12 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 01 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 02 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 04 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 07 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 08 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 09 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 10 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population Le-Puy-en-Velay, maternal family 10 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 03 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 03 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 05 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 05 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 05 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 05 , halfsib d

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 06 , halfsib a

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 06 , halfsib b

text: Dittrichia graveolens

text: Dittrichia graveolens


text: ETH ZURICH

text: public

text: Population la Roquebrussanne, maternal family 06 , halfsib c

text: Dittrichia graveolens

text: Dittrichia graveolens


text: Epithelial cell

text: Cecum

text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: house mouse

characteristic: common name

text: Female

characteristic: gender

text: Mus musculus

text: Mus musculus

characteristic: subject id

text: Mus musculus uninfected 1


text: Epithelial cell

text: Cecum

text: female

text: Mus musculus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Female

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus uninfected 2


text: Epithelial cell

text: Cecum

text: female

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Female

text: Mus musculus

text: Mus musculus

text: Mus musculus infected 1


text: Epithelial cell

text: Cecum

text: female

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Female

text: Mus musculus

text: Mus musculus

text: Mus musculus infected 2


text: EMBL

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Flies expressing EGFP-CBP20 under control of a mesodermal enhancer were grown in population cages at 25 degrees. After three/four 1 hr pre-lays, the flies were allowed to lay for 1 or 2 hrs, after which the embryos were aged at the same temperature to the appropriate time-point. The embryos were then dochorionated using 50% bleach, washed with distillate water and PBT (PBS + 1% Triton X-100), and directly subjected to homogenization on ice. All steps were done at 4C. 500 ul of embryo suspension was added to 6.5 ml Schneider???s media with Actinomycin D in a 15 ml dounce homogenizer on ice and dounced with a loose pestle 7 times, in aliquots of less than 0.0625 g of embryos. Material from two douncing rounds was combined in one 15 ml tube and centrifuged at 600 rpm for 5 min at 4C. Supernatant was transferred into a clean tube and centrifuged at 1700 rpm for 10 min at 4C. Supernatant was discarded, and 250 ul of Schneider???s media complemented with 8% fetal bovine serum and 1 ug/ml of Actinomycin D was added to the pellet. All resuspended pellets were combined into a single tube, cells were gently passed through a 18-gauge needle 5 times and sieved through a 40 um sieve into a 50 ml tube. Approximately 5% of the total sample was transferred into a RNAse free 1.5ml tube and centrifuged at 800 g for 10 min at 4C. 600 ul TRIzol was added to the pellet and saved as unsorted sample. Remaining sample was used for fluorescence activated cell sorting (FACS). Cell sorter (MoFlo, Beckman Coulter) was precooled at 4C and run with 70 um nozzle and 5,000 ??? 10,000 cells per second. Sorted cells were collected in 5 ml round bottom polypropylene tubes in 500 ul Seecof saline (6 mM Na2HPO4, 3.67 mM KH2PO4, 106 mM NaCl, 26.8 mM KCl, 6.4 mM MgCl2, 2.25 mM CaCl2, pH 6.8) supplemented with 0.1 U/ul RNAse inhibitor. Sorted cells were aliquoted in low binding RNAse free tubes and centrifuged at 800 g 10 min at 4C. Pellet was resuspended in 150 ul TRIzol LS and all samples were pooled into a single tube before proceeding with RNA isolation. RNA isolation was done according to manufacturer???s instructions for TRIzol LS, including overnight precipitation step with 1 ul glycogen at -80C. RNA was treated with RNAse free DNAse I (Roche) for 30 min and purified with Agencourt RNAclean XP beads (Beckam Coulter) according to manufacturer???s instructions. 'U' extracts: RNA extracted from cells before sorting (see below) 'S' extracts: RNA extracted from sorted cells. Production of libraries containing 27bp-tags from 5' ends of capped transcripts. Taken from Takahashi et al, Nat Prot 2012. Briefly, 1-5ug of total RNA are retrotranscribed with a primer containing a common sequence followed by 15 Ns. The RNA-cDNA duplexed is subjected to in-vitro biotinylation of the 5'cap. Then the single-stranded RNA is degraded with RNaseI and the remaining biotinylated (capped) RNA-cDNA duplexes are pull-down with streptavidin-conjugated Dynabeads. cDNA is released through NaOH treatment, and then a barcoded 5'adapter is ligated to the 3'end of the single strand cDNA. Non-ligated adapter is removed by purifying twice with AMPure XP beads. Second strand is synthesized using a primer corresponding to the 5'adapter. Then, 27bp-long tags are generated by digestion with EcoP15I, a type III restriction enzyme. The two matched restriction sites are in the 5'adapter and in the RT primer sequences. A common 3'adapter is then ligated to the resulting fragments. The libraries are amplified by PCR, tipically for 8-15 cycles. After PCR, primers are removed with exonuclease treatment, and the double strand fragments are purified both with QIAquick columns and AMPure XP beads to remove unwanted adapter dimers.

text: stage 8-9

characteristic: developmental stage

text: egg laying

characteristic: initial time point

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mesoderm

characteristic: organism part

text: Drosophila melanogaster

characteristic: strain


text: EMBL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: stage 8-9

text: egg laying

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL

text: public

text: ArrayExpress

text: fruit fly

text: stage 10-11

text: egg laying

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: mesoderm

text: Drosophila melanogaster


characteristic: External Id

text: EMBL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fruit fly

text: stage 5-7

text: egg laying

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mesoderm

text: Drosophila melanogaster


text: EMBL

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: stage 10-11

characteristic: developmental stage

text: egg laying

characteristic: initial time point

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster


text: EMBL

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: fruit fly

text: stage 5-7

text: egg laying

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole embryo

characteristic: organism part

text: Drosophila melanogaster

characteristic: strain


text: synthetic construct

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: Submitter Id

text: synthetic construct

characteristic: common name

text: synthetic construct

text: synthetic construct

characteristic: subject id

characteristic: title


text: Mouse

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

text: Female

characteristic: gender

text: Mus musculus

characteristic: organism

text: Mouse

characteristic: phenotype

text: Mus musculus


text: Mouse

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Male

text: Mus musculus

text: Mouse

text: Mus musculus


text: Tail

text: male

text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Male

text: Mus musculus

text: Tail

text: Mus musculus


text: Tail

characteristic: ArrayExpress-PHENOTYPE

text: female

text: Mus musculus

text: SC

text: public

text: house mouse

text: Female

text: Mus musculus

text: Tail

text: Mus musculus

characteristic: subject id

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CHENGDU INSTITUTE OF BIOLOGY

text: public

tag: Namespace:CHENGDU INSTITUTE OF BIOLOGY

characteristic: collection date

characteristic: depth

text: Forest soil

characteristic: environment (biome)

text: Forest

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: soil

characteristic: environmental package

text: China

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: Diazotrophs

characteristic: project name

text: soil metagenome

text: amplicon sequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Diazotrophs in forest soil


Nicotiana tabacum

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nicotiana tabacum

characteristic: Submitter Id

text: common tobacco

characteristic: common name

text: Plants grown in glasshouse under standard conditions. gDNA isolated form healthy leaves of young plants (4-weeks old)

characteristic: description

characteristic: estimated size

text: United Kingdom

text: glasshouse

characteristic: growth facility

text: normal growth condition

characteristic: isolation and growth condition

characteristic: number of replicons

text: Nicotiana tabacum

characteristic: organism

text: fully grown plant

characteristic: plant developmental stage

text: standard compost

characteristic: plant growth medium

text: Leaf

characteristic: plant structure

text: tetraploid

characteristic: ploidy

text: healthy

characteristic: sample health state

text: Nicotiana tabacum

text: Nicotiana tabacum cv. Petit Gerard

characteristic: title


Nicotiana sylvestris

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Nicotiana sylvestris

characteristic: collection date

text: wood tobacco

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: glasshouse

text: normal growth condition

text: Nicotiana sylvestris

text: fully grown plant

text: standard compost

text: Leaf

text: diploid

text: healthy

text: Nicotiana sylvestris

text: Nicotiana sylvestris


Nicotiana tomentosiformis

text: JIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nicotiana tomentosiformis

characteristic: Submitter Id

characteristic: description

characteristic: estimated size

text: United Kingdom

text: glasshouse

characteristic: growth facility

text: normal growth condition

characteristic: isolation and growth condition

characteristic: number of replicons

text: Nicotiana tomentosiformis

characteristic: organism

text: fully grown plant

characteristic: plant developmental stage

text: standard compost

text: Leaf

text: diploid

text: healthy

text: Nicotiana tomentosiformis

text: Nicotiana tomentosiformis


Solanum tuberosum Group Phureja

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

text: public

text: Solanum tuberosum Group Phureja

text: potato

characteristic: common name

text: United Kingdom

text: glasshouse

text: normal growth condition

text: Solanum tuberosum

text: fully grown plant

text: standard compost

characteristic: plant growth medium

text: Leaf

characteristic: plant structure

text: monoploid

characteristic: ploidy

text: healthy

characteristic: sample health state

text: Solanum tuberosum

text: Solanum tuberosum, Group Phureja, Clone DM1-3 516R44

characteristic: title


Solanum tuberosum cv Maris Piper

text: JIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Solanum tuberosum cv Maris Piper

characteristic: Submitter Id

characteristic: collection date

text: potato

characteristic: description

characteristic: estimated size

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: glasshouse

text: normal growth condition

text: Solanum tuberosum

text: fully grown plant

text: standard compost

text: Leaf

text: tetraploid

text: healthy

text: Solanum tuberosum

text: Solanum tuberosum, cv Maris Piper


Solanum lycopersicum accession FLA8059

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

text: public

text: Solanum lycopersicum accession FLA8059

text: tomato

characteristic: common name

text: United Kingdom

text: glasshouse

characteristic: growth facility

text: normal growth condition

characteristic: isolation and growth condition

characteristic: number of replicons

text: Solanum lycopersicum

characteristic: organism

text: fully grown plant

characteristic: plant developmental stage

text: standard compost

text: Leaf

text: diploid

text: healthy

text: Solanum lycopersicum

text: Solanum lycopersicum, accession FLA8059


Solanum americanum accession 944750095

text: JIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Solanum americanum accession 944750095

characteristic: Submitter Id

characteristic: collection date

text: United Kingdom

text: glasshouse

text: normal growth condition

text: Solanum americanum

text: fully grown plant

text: standard compost

characteristic: plant growth medium

text: Leaf

characteristic: plant structure

text: diploid

characteristic: ploidy

text: healthy

characteristic: sample health state

text: Solanum americanum

text: Solanum americanum, accession 944750095

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

text: public

characteristic: description

characteristic: estimated size

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: glasshouse

text: normal growth condition

text: Nicotiana benthamiana

text: fully grown plant

text: standard compost

text: Leaf

text: tetraploid

text: healthy

text: Nicotiana benthamiana

text: Nicotiana benthamiana


text: HARVARD SCHOOL OF PUBLIC HEALTH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:HARVARD SCHOOL OF PUBLIC HEALTH

characteristic: Submitter Id

text: MalariaGEN

characteristic: collecting institution

text: TRAC (Tracking Resistance to Artemisinin Collaboration)

characteristic: collector name

text: malaria parasite P. falciparum

characteristic: common name

text: Asexual Stage

characteristic: dev stage

text: Thailand

characteristic: isolate

text: peripheral blood

characteristic: isolation source host associated

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: TRAC PPQR P falciparum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: AGRICULTURE & AGRI-FOOD CANADA

characteristic: INSDC center name

text: public

tag: Namespace:AGRICULTURE & AGRI-FOOD CANADA

characteristic: cultivar

text: Transcriptome analysis of Pinto Bean seed coat

characteristic: description

text: 150mg seed stage

text: Phaseolus vulgaris

text: seed coat

characteristic: organism part

text: Phaseolus vulgaris

text: Phaeolus vulgaris L. seed coat

characteristic: title


text: AGRICULTURE & AGRI-FOOD CANADA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: CDC

text: 150mg seed stage

text: Phaseolus vulgaris

text: seed coat

text: Phaseolus vulgaris

text: Phaeolus vulgaris L. seed coat


text: JIC

text: public

text: Hyaloperonospora arabidopsidis

text: Hyaloperonospora arabidopsidis

text: Resequencing of Hyaloperonospora arabidopsidis isolates


Perchlorate_Rep_1_port_1_day 0

text: UC BERKELEY

text: public

text: Perchlorate_Rep_1_port_1_day 0

characteristic: collection date

text: 16S rRNA gene amplicon bacterial community sequences in the presence of sulfate

text: Crude oil contaminated sand

characteristic: environment (biome)

text: hydrocarbon contaminated sample

characteristic: environment (feature)

text: crude oil contaminated sand

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: metagenome

text: Microbial souring control

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Pulsed perchlorate treatment


Perchlorate_Rep_1_port_2_day 0

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UC BERKELEY

characteristic: INSDC center name

text: public

text: Perchlorate_Rep_1_port_2_day 0

characteristic: description

text: Crude oil contaminated sand

text: hydrocarbon contaminated sample

text: crude oil contaminated sand

text: USA

text: metagenome

text: metagenome

characteristic: organism

text: Microbial souring control

text: metagenome

text: Illumina MiSeq

text: Pulsed perchlorate treatment

characteristic: title


Perchlorate_Rep_1_port_3_day 0

text: UC BERKELEY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Perchlorate_Rep_1_port_3_day 0

characteristic: Submitter Id

characteristic: collection date

text: Crude oil contaminated sand

characteristic: environment (biome)

text: hydrocarbon contaminated sample

characteristic: environment (feature)

text: crude oil contaminated sand

text: USA

text: metagenome

text: metagenome

text: Microbial souring control

text: metagenome

text: Illumina MiSeq

text: Pulsed perchlorate treatment


Perchlorate_Rep_2_port_1_day 0

text: UC BERKELEY

text: public

text: Perchlorate_Rep_2_port_1_day 0

text: Crude oil contaminated sand

text: hydrocarbon contaminated sample

text: crude oil contaminated sand

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

text: Microbial souring control

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Pulsed perchlorate treatment


Perchlorate_Rep_2_port_2_day 0

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UC BERKELEY

characteristic: INSDC center name

text: public

text: Perchlorate_Rep_2_port_2_day 0

characteristic: description

text: Crude oil contaminated sand

text: hydrocarbon contaminated sample

text: crude oil contaminated sand

text: USA

text: metagenome

text: metagenome

characteristic: organism

text: Microbial souring control

text: metagenome

text: Illumina MiSeq

text: Pulsed perchlorate treatment

characteristic: title


Perchlorate_Rep_2_port_3_day 0

text: UC BERKELEY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Perchlorate_Rep_2_port_3_day 0

characteristic: Submitter Id

characteristic: collection date

text: Crude oil contaminated sand

characteristic: environment (biome)

text: hydrocarbon contaminated sample

characteristic: environment (feature)

text: crude oil contaminated sand

text: USA

text: metagenome

text: metagenome

text: Microbial souring control

text: metagenome

text: Illumina MiSeq

text: Pulsed perchlorate treatment


Perchloratel_Rep_1_port_1_day 40

text: UC BERKELEY

text: public

text: Perchloratel_Rep_1_port_1_day 40

text: Crude oil contaminated sand

text: hydrocarbon contaminated sample

text: crude oil contaminated sand

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

text: Microbial souring control

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Pulsed perchlorate treatment


Perchlorate_Rep_1_port_2_day 40

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UC BERKELEY

characteristic: INSDC center name

text: public

text: Perchlorate_Rep_1_port_2_day 40

characteristic: description

text: Crude oil contaminated sand

text: hydrocarbon contaminated sample

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