characteristic: Citation

characteristic: Event Date/Time End

text: public

characteristic: Latitude End

characteristic: Longitude End

unit: practical salinity unit

characteristic: Salinity Sensor

tag: Namespace:PANGAEA Data Publisher for Earth and Environmental Science

unit: m

characteristic: depth

text: This sample (EMOSE_N010000413) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(1L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000990,EMOSE_N010000419

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(1L-on-sterivex)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane. A volume of 1 Litres was filtered and the Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane was packaged in a sterile Whirl-pack bag, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 14 minutes, starting at 11:04 and ending at 11:18 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

unit: ºC

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(1L-on-sterivex)_W>0.22_N010000413


status: PUBLIC

tag: attribute

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

unit: DD

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000990) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(1L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000413,EMOSE_N010000419

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(1L-on-sterivex)_W>0.22_N010000990

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000419) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(1L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000413,EMOSE_N010000990

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(1L-on-sterivex)_W>0.22_N010000419


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000414) was created in silico using samples EMOSE_N010000414, EMOSE_N010000416, EMOSE_N010000415 and EMOSE_N010000417. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (100 ng each) were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N020000421,EMOSE_N020000991.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(2.5L-on-sterivex)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane. A volume of 10 Litres was filtered and the Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane was packaged in a sterile Whirl-pack bag, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 58 minutes, starting at 11:53 and ending at 12:51 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N020000414

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000414) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(2.5L-on-sterivex)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane. A volume of 2.5 Litres was filtered and the Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane was packaged in a sterile Whirl-pack bag, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 58 minutes, starting at 11:53 and ending at 12:51 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000414


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000416) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000416

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000415) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000415


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000417) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000417

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N020000421) was created in silico using samples EMOSE_N010000421/EMOSE_N010000423/EMOSE_N010000420/EMOSE_N010000422. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (100 ng each) were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N020000414,EMOSE_N020000991

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(2.5L-on-sterivex)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane. A volume of 2.5 Litres was filtered and the Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane was packaged in a sterile Whirl-pack bag, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 58 minutes, starting at 13:12 and ending at 14:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N020000421


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000421) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000421

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000423) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000423


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000420) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000420

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000422) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000992,EMOSE_N010000994

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000422


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000991) was created in silico using samples EMOSE_N010000991, EMOSE_N010000993, EMOSE_N010000992 and EMOSE_N010000994. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (100 ng each) are pooled (R01.1-R01.4) before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N020000421,EMOSE_N020000414

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(2.5L-on-sterivex)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane. A volume of 2.5 Litres was filtered and the Sterivex filter unit (ref:SVGPB1010) with a Millipore polyethersulfone membrane was packaged in a sterile Whirl-pack bag, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 54 minutes, starting at 14:26 and ending at 15:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N020000991

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000991) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000991


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000993) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992,EMOSE_N010000994

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000993

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000992) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000994

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000992


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000994) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(2.5L-on-sterivex)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000414,EMOSE_N010000416,EMOSE_N010000421,EMOSE_N010000423,EMOSE_N010000991,EMOSE_N010000993,EMOSE_N010000415,EMOSE_N010000417,EMOSE_N010000420,EMOSE_N010000422,EMOSE_N010000992

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(2.5L-on-sterivex)_W>0.22_N010000994

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000418) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000424,EMOSE_N010000995

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 11:29 and ending at 11:34 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000418


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000424) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000995

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 12:02 and ending at 12:09 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000424

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000407) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000424

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W>0.22, the sample material was not pre-filtered and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 12:37 and ending at 12:43 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>0.22_N010000407


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000385) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 30 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 91 minutes, starting at 11:25 and ending at 12:56 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W>20_N010000385

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000368) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000424,EMOSE_N010000995

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 11:30 and ending at 11:35 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W0.22-3_N010000368


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000401) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000995

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 12:03 and ending at 12:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W0.22-3_N010000401

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000995) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000424

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 12:50 and ending at 12:56 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W0.22-3_N010000995


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000369) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000424,EMOSE_N010000995

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 5 minutes, starting at 11:30 and ending at 11:35 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W3-20_N010000369

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000402) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000995

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(10L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 7 minutes, starting at 12:03 and ending at 12:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W3-20_N010000402


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000408) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(10L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000418,EMOSE_N010000424

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(10L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 6 minutes, starting at 12:50 and ending at 12:56 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(10L-on-membrane)_W3-20_N010000408

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000370) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-0.8, the sample material was pre-filtered on 0.8-micrometres, using a 142mm membrane, and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 225 minutes, starting at 11:25 and ending at 15:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-0.8_N010000370


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000371) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.8-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W0.8-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 225 minutes, starting at 11:25 and ending at 15:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.8-20_N010000371

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000386) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 120? Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 225 minutes, starting at 11:25 and ending at 15:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000386


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000384) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 152 minutes, starting at 13:58 and ending at 16:30 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000384

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000383) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000378,EMOSE_N010000385

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 60 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 167 minutes, starting at 17:03 and ending at 19:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000383