characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: 16 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Centre for Genome Enabled Biology and Medicine University of Aberdeen

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: Fetuses collected by trained nurses and processed immediately. Fetal weight, morphology, sex, crown-rump length, ano-genital distance and supine distance were recorded. Blood samples were collected by inserting a needle into the chest cavity towards the heart, under the ribs. The abdomen of the fetus was entered using scissors and tweezers to cut down the midline of the abdomen and under the rib cage to expose the internal organs. The liver was removed, placed onto autoclaved lint-free paper to remove excess connective tissue and blood. The liver was weighed. Three 30 mg pieces of liver were cut from the main lobe and snap frozen in separate tubes. A larger piece from the main lobe was fixed in NBF for sectioning. The remaining liver was snap frozen in a small plastic sleeve. RNA was extracted from the liver samples using the Qiagen AllPrep DNA/RNA/Protein Mini kit as per manufacturer's instructions. Illumina TruSeq Stranded mRNA Library Prep Kit

characteristic: description

text: 12 weeks gestation

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: Centre for Genome Enabled Biology and Medicine University of Aberdeen

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: 12 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

characteristic: environmental history

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

text: ArrayExpress

text: human

text: 13 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: 17 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: 12 weeks gestation

characteristic: developmental stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


text: public

text: ArrayExpress

text: human

text: 16 weeks gestation

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: 17 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

characteristic: environmental history

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

text: ArrayExpress

text: human

text: 15 weeks gestation

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: 14 weeks gestation

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: 14 weeks gestation

characteristic: developmental stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: public

text: ArrayExpress

text: human

text: 13 weeks gestation

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: 17 weeks gestation

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

text: ArrayExpress

text: human

text: 16 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

characteristic: environmental history

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: 15 weeks gestation

text: normal

text: exposure to cigarette smoke in utero

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: 19 weeks gestation

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


text: public

text: ArrayExpress

text: human

text: 19 weeks gestation

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: 18 weeks gestation

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

text: ArrayExpress

text: human

text: not available

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: not available

text: normal

text: exposure to cigarette smoke in utero

characteristic: environmental history

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: not available

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: title


text: public

text: ArrayExpress

text: human

characteristic: description

text: not available

characteristic: developmental stage

text: normal

characteristic: disease

text: exposure to cigarette smoke in utero

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


85-d WGS

characteristic: ENA-LAST-UPDATE

text: Centre for Organismal Studies

text: public

text: 85-d WGS

tag: Namespace:Centre for Organismal Studies

text: Arabidopsis lyrata and A. arenosa genomes sequenced to study adaptive introgression

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


85-e WGS

text: Centre for Organismal Studies

text: public

text: 85-e WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


55-b WGS

text: Centre for Organismal Studies

text: public

text: 55-b WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


55-c WGS

text: Centre for Organismal Studies

text: public

text: 55-c WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


137-e WGS

text: Centre for Organismal Studies

text: public

text: 137-e WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


137-g WGS

text: Centre for Organismal Studies

text: public

text: 137-g WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


37-c WGS

text: Centre for Organismal Studies

text: public

text: 37-c WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


37-h WGS

text: Centre for Organismal Studies

text: public

text: 37-h WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-m WGS

text: Centre for Organismal Studies

text: public

text: 88-m WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-o WGS

text: Centre for Organismal Studies

text: public

text: 88-o WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-p WGS

text: Centre for Organismal Studies

text: public

text: 88-p WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-q WGS

text: Centre for Organismal Studies

text: public

text: 88-q WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-r WGS

text: Centre for Organismal Studies

text: public

text: 88-r WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-s WGS

text: Centre for Organismal Studies

text: public

text: 88-s WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-t WGS

text: Centre for Organismal Studies

text: public

text: 88-t WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-u WGS

text: Centre for Organismal Studies

text: public

text: 88-u WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


88-v WGS

text: Centre for Organismal Studies

text: public

text: 88-v WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


74-4 WGS

text: Centre for Organismal Studies

text: public

text: 74-4 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


74-6 WGS

text: Centre for Organismal Studies

text: public

text: 74-6 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


74-9 WGS

text: Centre for Organismal Studies

text: public

text: 74-9 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


115-10 WGS

text: Centre for Organismal Studies

text: public

text: 115-10 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


115-1 WGS

text: Centre for Organismal Studies

text: public

text: 115-1 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


115-3 WGS

text: Centre for Organismal Studies

text: public

text: 115-3 WGS

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea

text: Arabidopsis lyrata subsp. petraea


201GM-004 WGS

text: Centre for Organismal Studies

text: public

text: 201GM-004 WGS

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii


201GM-017 WGS

text: Centre for Organismal Studies

text: public

text: 201GM-017 WGS

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii


118GM-012 WGS

text: Centre for Organismal Studies

text: public

text: 118GM-012 WGS

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii


118GM-018 WGS

text: Centre for Organismal Studies

text: public

text: 118GM-018 WGS

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii


402140-e WGS

text: Centre for Organismal Studies

text: public

text: 402140-e WGS

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii

text: Arabidopsis arenosa subsp. borbasii


text: INRA Angers

text: public

tag: Namespace:INRA Angers

characteristic: collection date

text: In this project, we aim to apply metagenomics approaches to uncover the function of the seed microbiota and its changes during germination and emergence. We will try to reveal through metagenomics the genetic content of this microbiota in seeds, germinating seeds and seedlings from 2 different plant families (Fabaceae and Brassicaceae).

text: seed

characteristic: environment (biome)

text: comercial inoculum

characteristic: environment (feature)

text: comercial inoculum

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Phaseoleae environmental sample

text: plant-associated

characteristic: plant-associated environmental package

text: MetaSeed

characteristic: project name

text: Phaseoleae environmental sample

text: Shotgun Illumina sequencing

characteristic: sequencing method

text: Seed, germinating seeds and seedlings metagenomes from Brasicca napus and Phaseolus vulgaris


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: INRA Angers

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seed

text: comercial inoculum

text: comercial inoculum

text: France

text: metagenome

text: Raphanus sativas chrysovirus 1

text: plant-associated

text: MetaSeed

text: Raphanus sativas chrysovirus 1

text: Shotgun Illumina sequencing

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Animal and Plant Health Agency (APHA)

text: public

tag: Namespace:Animal and Plant Health Agency (APHA)

text: APHA

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Sus domesticus

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: Salmonella enterica subsp. enterica serovar Typhimurium

characteristic: organism

text: Typhimurium

characteristic: serovar

text: Salmonella isolate


text: 29358649; 31736907; 34461978

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: LSHTM

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: MTB_Saudi__1299_MTB-Pool59_3499__L3_AACGTGAT_L003

text: Mycobacterium

characteristic: genus

text: Homo sapiens

characteristic: host

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

type: AMR curated data - CABBAGE

value: ethambutol

value: susceptible

value: Mycobacterium tuberculosis

value: rifampin

value: streptomycin

value: isoniazid


text: 29358649; 31736907; 33075053; 34461978

characteristic: ENA-LAST-UPDATE

text: LSHTM

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: MTB_Saudi__1352_MTB-Pool59_3499__L3_AAACATCG_L003

characteristic: Submitter Id

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

characteristic: title

value: broth dilution


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1356_MTB-Pool59_3499__L3_ATGCCTAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907

text: LSHTM

text: public

text: MTB_Saudi__1384_MTB-Pool59_3499__L3_AGTGGTCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907

text: LSHTM

text: public

text: MTB_Saudi__1391_MTB-Pool59_3499__L3_ACCACTGT_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1406_MTB-Pool59_3499__L3_ACATTGGC_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907

text: LSHTM

text: public

text: MTB_Saudi__1434_MTB-Pool59_3499__L3_CAGATCTG_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1446_MTB-Pool59_3499__L3_CATCAAGT_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1459_MTB-Pool59_3499__L3_CGCTGATC_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

value: resistant


text: 29358649; 31736907; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1507_MTB-Pool59_3499__L3_ACAAGCTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1509_MTB-Pool59_3499__L3_CTGTAGCC_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1527_MTB-Pool59_3499__L3_AGTACAAG_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1532_MTB-Pool59_3499__L3_AACAACCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1547_MTB-Pool59_3499__L3_AACCGAGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1617_MTB-Pool59_3499__L3_AAGACGGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907

text: LSHTM

text: public

text: MTB_Saudi__1625_MTB-Pool59_3499__L3_AAGGTACA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1712_MTB-Pool59_3499__L3_ACACAGAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1781_MTB-Pool59_3499__L3_ACAGCAGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1794_MTB-Pool59_3499__L3_ACCTCCAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1800_MTB-Pool59_3499__L3_ACGCTCGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1804_MTB-Pool59_3499__L3_ACGTATCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1829_MTB-Pool59_3499__L3_ACTATGCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1843_MTB-Pool59_3499__L3_AGAGTCAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1865_MTB-Pool59_3499__L3_AGATCGCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1891_MTB-Pool59_3499__L3_AGCAGGAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1901_MTB-Pool59_3499__L3_AGTCACTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1903_MTB-Pool59_3499__L3_ATCCTGTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1910_MTB-Pool59_3499__L3_ATTGAGGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907

text: LSHTM

text: public

text: MTB_Saudi__1921_MTB-Pool59_3499__L3_CAACCACA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1924_MTB-Pool59_3499__L3_GACTAGTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1933_MTB-Pool59_3499__L3_CAATGGAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__1940_MTB-Pool59_3499__L3_CACTTCGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1942_MTB-Pool59_3499__L3_CAGCGTTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1949_MTB-Pool59_3499__L3_CATACCAA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1951_MTB-Pool59_3499__L3_CCAGTTCA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__1991_MTB-Pool59_3499__L3_CCGAAGTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__2000_MTB-Pool59_3499__L3_CCGTGAGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053; 34461978

text: LSHTM

text: public

text: MTB_Saudi__2010_MTB-Pool59_3499__L3_CCTCCTGA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 33075053

text: LSHTM

text: public

text: MTB_Saudi__2015_MTB-Pool59_3499__L3_CGAACTTA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: 29358649; 31736907; 34461978

text: LSHTM

text: public

text: MTB_Saudi__2057_MTB-Pool59_3499__L3_CGCATACA_L003

text: Mycobacterium

text: Homo sapiens

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: UCSDMI

text: public

characteristic: age

text: weeks

characteristic: age units

characteristic: anonymized name

text: UBERON:feces

characteristic: body habitat

text: UBERON:material entity in digestive tract

characteristic: body product

text: UBERON:feces

characteristic: body site

text: ileal content from mice

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: intestine environment

text: USA:CA:Davis

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: mouse

characteristic: host common name

text: Mus musculus

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: mouse gut metagenome

text: UC Davis Marco lab

text: TRUE

text: ileal content

characteristic: sample type

text: mouse gut metagenome

text: male

characteristic: sex

text: plantaricin effect on gut microbiota

characteristic: weight

text: g


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: weeks

characteristic: age units

characteristic: anonymized name

text: UBERON:feces

characteristic: body habitat

text: UBERON:lumen of gut

characteristic: body product

text: UBERON:feces

characteristic: body site

text: ileal content from mice

characteristic: description

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: intestine environment

text: USA:CA:Davis

text: Missing: Not provided

text: Missing: Not provided

text: mouse

text: Mus musculus

text: adult

text: mouse gut metagenome

characteristic: organism

text: UC Davis Marco lab

text: TRUE

text: ileal content

text: mouse gut metagenome

text: male

characteristic: title

text: plantaricin effect on gut microbiota

text: g


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

text: weeks

text: UBERON:feces

text: UBERON:lumen of gut

text: UBERON:feces

text: ileal content from mice

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: intestine environment

text: USA:CA:Davis

characteristic: geo loc name

text: Missing: Not provided

characteristic: height

text: Missing: Not provided

text: mouse

characteristic: host common name

text: Mus musculus

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: mouse gut metagenome

text: UC Davis Marco lab

text: TRUE

text: ileal content

characteristic: sample type

text: mouse gut metagenome

text: male

characteristic: sex

text: plantaricin effect on gut microbiota

characteristic: weight

text: g


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Missing: Not provided

characteristic: age

text: Missing: Not provided

characteristic: age units

text: NC

characteristic: anonymized name

text: UBERON:feces

characteristic: body habitat

text: UBERON:lumen of gut

characteristic: body product

text: UBERON:feces

characteristic: body site

text: ileal content from mice

characteristic: description

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: intestine environment

text: USA:CA:Davis

text: Missing: Not provided

text: Missing: Not provided

text: mouse

text: Mus musculus

text: NC

text: Missing: Not provided

text: mouse gut metagenome

characteristic: organism

text: UC Davis Marco lab

text: TRUE

text: ileal content

text: mouse gut metagenome

text: Missing: Not provided

characteristic: title

text: plantaricin effect on gut microbiota

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MAX PLANCK INSTITUE OF CHEMCIAL ECOLOGY

text: public

tag: Namespace:MAX PLANCK INSTITUE OF CHEMCIAL ECOLOGY

characteristic: collection date

text: Great Basin Desert

characteristic: environment (biome)

text: New Plot

characteristic: environment (feature)

text: roots, leaves, soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Nicotiana attenuata

text: plant-associated

characteristic: plant-associated environmental package

text: root endophytes

characteristic: project name

text: Nicotiana attenuata

characteristic: sequencing method

text: EV ICE pilot


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Biochemistry and Molecular Biology, Biocenter, University of Wurzburg

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: fruit fly

characteristic: common name

text: Protocols: S2 cells were left untreated or incubated with dsRNA against Atu or Myc. The cells were harvested 48h later. (HA-Leo1 expression was induced by addition of 125 M CuSo4. Cells were harvested 24h later) Cells were cross-linked with 1% formaldehyde at 37C for 10 min and the reaction was stopped with 50 mM glycine. Cells were lysed and nuclei were resuspended in RIPA buffer. Sonication with a Branson sonifier was carried out until the majority of fragments showed nucleosomal size. Chromatin was immunoprecipitated with Myc antibody (rabbit -Myc) or control IgG from rabbit (Sigma) which were coupled to Protein A/G-Dynabeads (Invitrogen). DNA was purified with Phenol- Chloroform after elution of the bound chromatin with 1% SDS and reversion of the crosslink. Sequencing libraries were prepared with the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #6240S/L) following the instruction manual. The cleanup steps were carried out using MinElute PCR Purification Kit (Qiagen) followed by gel electrophoresis for size selection.

characteristic: description

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: AtuKD

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Biochemistry and Molecular Biology, Biocenter, University of Wurzburg

text: public

text: ArrayExpress

text: fruit fly

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ctr

characteristic: title


text: public

text: ArrayExpress

text: fruit fly

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: MycKD


text: Shandong Agricultural University

text: public

text: ArrayExpress

text: bread wheat

text: Luozhen No.1

characteristic: cultivar

text: Protocols: Having cut both side of embryo and another, the pericarp of the seed were stripped and frozen in liquid nitrogen and stored at 80oC until RNA extraction. Plants of purple-grain wheat line Luozhen No.1 were grown under natural field conditions at Shandong Agricultural University Experimental Station (36169 N, 117169 E), Taian, China during October, 2015 to June, 2016. The spikes of purple-grain wheat Luozhen No.1 were bagged with four-layer Kraft paper bags after pollination. Total RNAs were extracted from pericarp of purple-grain wheat using an RNeasy Plant Mini Kit (Cat. No. 74904, Qiagen, Valencia, CA, USA). At least 500 ng total RNA was extracted from each material used for library construction. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. After agarose gel electrophoresis, the suitable fragments are selected for the PCR amplification as templates. During the quality control steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqTM 2000.

text: fruit ripening stage

characteristic: developmental stage

text: continuous dark (no light) regimen

characteristic: environmental stress

text: field study

characteristic: growth condition

text: Triticum aestivum

text: Triticum aestivum

text: pericarp

characteristic: organism part

text: 15 days after pollination

characteristic: sampling time point

text: Triticum aestivum


characteristic: External Id

text: Shandong Agricultural University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: bread wheat

text: Luozhen No.1

text: fruit ripening stage

text: continuous dark (no light) regimen

text: field study

text: Triticum aestivum

text: Triticum aestivum

text: pericarp

text: 20 days after pollination

text: Triticum aestivum


characteristic: ENA-CHECKLIST

text: Shandong Agricultural University

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bread wheat

characteristic: common name

text: Luozhen No.1

characteristic: description

text: fruit ripening stage

text: field study

text: Triticum aestivum

text: Triticum aestivum

text: pericarp

text: 15 days after pollination

text: Triticum aestivum


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Shandong Agricultural University

text: public

text: ArrayExpress

text: bread wheat

text: Luozhen No.1

characteristic: cultivar

text: fruit ripening stage

text: field study

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: pericarp

text: 20 days after pollination

text: Triticum aestivum

characteristic: title


text: UPPSALA UNIVERISTY

text: public

tag: Namespace:UPPSALA UNIVERISTY

text: collared flycatcher

text: Ficedula albicollis

text: Ficedula albicollis

text: Male

characteristic: sex


characteristic: External Id

text: public

text: E-MTAB-6078:WL438_10hpf_Smarcd3F6_danrer_FACS-GFPposi_ATAC_R1

tag: Namespace:Genetics and Genome Biology Program, Hospital for Sick Children, Toronto, Canada; Department of Molecular Genetics, University of Toronto

unit: hour

characteristic: age

text: ArrayExpress

text: zebrafish

text: Protocols: Around 100 Tg(Smarcd3-F6: EGFP) embryos were dechorionated with pronase (Sigma, Cat# 11459643001) and transferred to a 1.5mL Eppendorf tube when they reached bud stage. After incubated in 200ul calcium-free Ringer solution (116 mM NaCl, 2.6mM KCl, 5mM HEPE, pH 7.0) for 5 min, embryos were transferred into a 24-well plate filled with 500 ul TrypLE solution (GIBOCO, TrypLE Express Enzyme, cat #: 12604-013) for dissociation at room temperature. Embryos were gently homogenized every 5min with P1000 tips. Dissociation was monitored under a dissection scope until most cells were in single-cell suspension. The cell suspension was transferred into 200ul ice-cold FBS to stop the reaction. Cells were centrifuged at 300g for 3min at 4 degree and washed with 500 ul ice-cold DMEM with 10% FBS before resuspended in 500 ul ice-cold DMEM with 1% FBS. Right before the FACS, cells were filtered through a 40um strainer and DAPI was added at a concentration of 5 ug/ml to exclude dead cells. FACS was performed on Beckman Coulter Mo Flo XDP or Mo Flo Astrios sorter with a 100um nozzle by the SickKids-UHN Flow and Mass Cytometry Facility. Cell-doublets and dead cells were excluded based on forward scatter, side scatter and DAPI channel. GFP+ and GFP- cells were sorted into 100% FBS and subjected to RNA-seq or ATAC-seq procedures immediately once sorting finished. 30,000-50,000 GFP+ cells and 100, 000 GFP- cells were usually collected in one sort. 30, 000-50,000 cells obtained from FACS were used for nuclei prep. Nuclei for ATAC-seq experiments were purified following the published protocol (doi:10.1002/0471142727.mb1300s82). ATAC-seq libraries were generated using the published protocol (doi:10.1002/0471142727.mb1300s82) with minor modifications. After tagmentation reaction, transposed DNA fragments were amplified using the following PCR condition (1 cycle of 72 C for 5 min and 98 C for 30 s, followed by 12 cycles of 98 C for 10 s, 63 C for 30 s and 72 C for 1 min). Amplified libraries were purified twice with Agencourt Ampure XP beads (beckman coulter, Cat#A63880) with a bead-to-sample ratio of 1.8:1. Qubit fluorometer and Aglient Bioanalyzer were used to check library quality and concentration.

text: gastrula bud

characteristic: developmental stage

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

text: GFP positive

characteristic: phenotype

characteristic: replicate

text: Danio rerio


characteristic: ENA-CHECKLIST

text: Genetics and Genome Biology Program, Hospital for Sick Children, Toronto, Canada; Department of Molecular Genetics, University of Toronto

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6078:WL439_10hpf_Smarcd3F6_danrer_FACS-GFPnega_ATAC_R1

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

text: gastrula bud

text: Danio rerio

text: Danio rerio

text: whole organism

text: GFP negative

text: Danio rerio


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-6078:WL440_10hpf_Smarcd3F6_danrer_FACS-GFPposi_ATAC_R2

text: ArrayExpress

characteristic: broker name

text: zebrafish

characteristic: common name

characteristic: description

text: gastrula bud

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

text: GFP positive

text: Danio rerio


characteristic: External Id

text: public

text: E-MTAB-6078:WL441_10hpf_Smarcd3F6_danrer_FACS-GFPnega_ATAC_R2

characteristic: age

text: ArrayExpress

text: zebrafish

text: gastrula bud

characteristic: developmental stage

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

text: GFP negative

text: Danio rerio

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: ArrayExpress

text: zebrafish

text: Protocols: Around 100 Tg(Smarcd3-F6: EGFP) embryos were dechorionated with pronase (Sigma, Cat# 11459643001) and transferred to a 1.5mL Eppendorf tube when they reached bud stage. After incubated in 200ul calcium-free Ringer solution (116 mM NaCl, 2.6mM KCl, 5mM HEPE, pH 7.0) for 5 min, embryos were transferred into a 24-well plate filled with 500 ul TrypLE solution (GIBOCO, TrypLE Express Enzyme, cat #: 12604-013) for dissociation at room temperature. Embryos were gently homogenized every 5min with P1000 tips. Dissociation was monitored under a dissection scope until most cells were in single-cell suspension. The cell suspension was transferred into 200ul ice-cold FBS to stop the reaction. Cells were centrifuged at 300g for 3min at 4 degree and washed with 500 ul ice-cold DMEM with 10% FBS before resuspended in 500 ul ice-cold DMEM with 1% FBS. Right before the FACS, cells were filtered through a 40um strainer and DAPI was added at a concentration of 5 ug/ml to exclude dead cells. FACS was performed on Beckman Coulter Mo Flo XDP or Mo Flo Astrios sorter with a 100um nozzle by the SickKids-UHN Flow and Mass Cytometry Facility. Cell-doublets and dead cells were excluded based on forward scatter, side scatter and DAPI channel. GFP+ and GFP- cells were sorted into 100% FBS and subjected to RNA-seq or procedures immediately once sorting finished. 30,000-50,000 GFP+ cells and 100, 000 GFP- cells were usually collected in one sort. Single-cell cDNA libraries were prepared using Fluidgim C1 system. After FACS, GFP positive cells were washed twice in DMEM with 3%FBS and filtered through a 40um cell strainer. Cells were adjusted to a concentration of 400-500 cells/ul before mixed with C1 suspension solution at a 5.2:4.8 ratio. Then 10ul final cell mixture was loaded into a C1 medium or small Chip. Cell capture was examined under a microscope and only wells with a single cell captured were included in library construction. Reverse transcription, PCR amplification and cDNA harvesting were performed as per manufactures instruction. Since the optimized capture efficiency of C1 chip for our cell is 30-40%, 96 single-cell cDNA libraries were collected from three batches of experiments at the end. Final sequencing libraries were made using Nextera XT DNA Sample Preparation Kit (Illumina, Cat# FC-131-1096).

text: gastrula bud

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

text: GFP postitive

characteristic: phenotype

text: fail

characteristic: post-analysis well quantity

text: Danio rerio

text: single cell

characteristic: well information


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

text: gastrula bud

text: Danio rerio

text: Danio rerio

text: whole organism

text: GFP postitive

text: pass

text: Danio rerio

text: single cell


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

characteristic: broker name

text: zebrafish

characteristic: common name

characteristic: description

text: gastrula bud

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

text: GFP postitive

text: Danio rerio

text: 4000 cells (tube control)


characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

characteristic: age

text: ArrayExpress

text: zebrafish

text: gastrula bud

characteristic: developmental stage

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

text: GFP negative

text: Danio rerio

characteristic: title

text: 4000 cells (tube control)


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

text: public

tag: Namespace:KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

unit: g

characteristic: amount or size of sample collected

characteristic: collection date

text: agricultural

characteristic: current land use

text: mediterranean shrubland

characteristic: environment (biome)

text: field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

unit: m

characteristic: geographic location (altitude)

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Crete

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified bacterium

characteristic: ph

text: Koiliaris

characteristic: project name

characteristic: sample weight for DNA extraction

text: unidentified bacterium

text: Illumina Miseq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: fresh

characteristic: storage conditions (fresh/frozen/other)

text: Soils from Koiliaris Mediterannean watershed


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: agricultural

characteristic: current land use

text: mediterranean shrubland

characteristic: environment (biome)

text: field

text: soil

text: Greece

text: Crete

text: metagenome

text: unidentified bacterium

characteristic: organism

text: Koiliaris

text: unidentified bacterium

text: Illumina Miseq

text: soil

text: fresh

characteristic: title


text: public

text: natural

text: mediterranean shrubland

text: field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: geographic location (altitude)

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Crete

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified bacterium

characteristic: ph

text: Koiliaris

characteristic: project name

characteristic: sample weight for DNA extraction

text: unidentified bacterium

text: Illumina Miseq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: fresh

characteristic: storage conditions (fresh/frozen/other)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUTE OF PHARMACEUTICAL SCIENCES

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:INSTITUTE OF PHARMACEUTICAL SCIENCES

characteristic: Submitter Id

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

characteristic: title


text: University Hospital Galway

text: public

tag: Namespace:University Hospital Galway

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Shigella flexneri 2a

text: Shigella flexneri 2a


characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Shigella flexneri 1b

text: Shigella flexneri 1b


text: University Hospital Galway

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Ireland

text: Homo sapiens

text: Human

text: Shigella dysenteriae

text: Shigella dysenteriae


text: public

tag: Namespace:Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust  Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: blood cell

characteristic: cell type

text: zebrafish

characteristic: common name

text: Protocols: A single kidney from heterozygote transgenic or wild-type fish was dissected and placed in ice cold PBS/5% fetal bovine serum. At the same time testes were dissected from the same fish. Single cell suspensions were generated by first passing through a 40 um strainer using the plunger of a 1 ml syringe as a pestle. These were then passed through a 20 um strainer before adding 4',6-diamidino-2-phenylindole (DAPI, Beckman Coulter, cat no B30437) for mCherry/dsRed2, or propidium iodide (PI, Sigma cat no P4864) for GFP/EGFP. Individual cells were index sorted into wells of a 96 well plate using a BD Influx Index Sorter. Kidney from a non-transgenic line was used as a control for gating. cDNA and library preparation was performed using the Smart-seq2 protocol 27, 28, with ERCC spike-in controls added at the same time as the oligo-dT and dNTP mixture (dilution 1:10^7, according to the manufacturer's instructions)

characteristic: description

text: adult

characteristic: developmental stage

characteristic: forward scatter

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: kidney

characteristic: organism part

characteristic: reporter fluorescence

text: Danio rerio

characteristic: side scatter

text: not OK

characteristic: single cell well quality

text: Tubingen Long Fin

characteristic: strain

text: 1 cell

characteristic: well information


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Haematology, University of Cambridge, Cambridge, UK Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 1QR, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: blood cell

text: zebrafish

text: adult

text: Danio rerio

characteristic: organism

text: Danio rerio

text: kidney

text: Danio rerio

text: OK

text: Tubingen Long Fin

characteristic: title

text: 1 cell


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: blood cell

characteristic: cell type

text: zebrafish

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

characteristic: forward scatter

text: Danio rerio

text: Danio rerio

text: kidney

text: Danio rerio

text: not OK

text: Tubingen Long Fin

text: 1 cell


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: blood cell

text: zebrafish

text: adult

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: kidney

characteristic: organism part

characteristic: reporter fluorescence

text: Danio rerio

characteristic: side scatter

text: not OK

characteristic: single cell well quality

text: Tubingen Long Fin

characteristic: strain

text: 0 cell

characteristic: well information


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: blood cell

text: zebrafish

text: adult

text: Danio rerio

characteristic: organism

text: Danio rerio

text: kidney

text: Danio rerio

text: not OK

text: Tubingen Long Fin

characteristic: title

text: 50 cell


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Michigan State University

text: public

tag: Namespace:Michigan State University

characteristic: Submitter Id

text: 6.6 � 1.0

unit: mEq/L

characteristic: alkalinity

characteristic: collection date

text: 249.8 � 74.9

unit: µmol/L

characteristic: dissolved organic carbon

text: 581.3 � 4.4

unit: µmol/kg

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Heavy

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

unit: mg

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.3 � 3.4

unit: ºC

characteristic: temperature

text: 22-M_Buckeye Trail Heavy Plot 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 593.8 � 68.8

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 11-28d_Englewood Moderate Plot 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 111_Englewood Moderate Plot 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 1.0

characteristic: alkalinity

characteristic: collection date

text: 249.8 � 74.9

characteristic: dissolved organic carbon

text: 581.3 � 4.4

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Heavy

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.3 � 3.4

text: 28-J_Buckeye Trail Heavy Plot 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 114_Englewood Moderate Plot 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.8 � 0.6

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 16.7

characteristic: dissolved organic carbon

text: 581.3 � 4.4

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.2

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.8 � 2.7

text: 57_Charleston Falls Moderate Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 176_Englewood Moderate Plot 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 593.8 � 68.8

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 143_Englewood Moderate Plot 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.9 � 0.1

text: 124.9 � 8.3

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Reference

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.2 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.4 � 2.0

characteristic: temperature

text: 77_Aullwood Reference Plot 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.5 � 0.7

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 58.3

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.2 � 3.6

text: 133_Englewood Reference Plot 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.8 � 0.6

text: 116.6 � 16.7

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.2

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.8 � 2.7

characteristic: temperature

text: 54_Charleston Falls Moderate Plot 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 1.0

characteristic: alkalinity

characteristic: collection date

text: 249.8 � 74.9

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Heavy

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.3 � 3.4

text: 182_Buckeye Trail Heavy Plot 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 1.0

text: 249.8 � 74.9

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Heavy

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.3 � 3.4

characteristic: temperature

text: 24-28d_Buckeye Trail Heavy Plot 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.5 � 0.7

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 58.3

characteristic: dissolved organic carbon

text: 581.3 � 56.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.2 � 3.6

text: 166_Englewood Reference Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 1.0

text: 249.8 � 74.9

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Heavy

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.3 � 3.4

characteristic: temperature

text: 184_Buckeye Trail Heavy Plot 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 581.3 � 4.4

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 112_Englewood Moderate Plot 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 1.0

text: 249.8 � 74.9

text: 550.0 � 75.0

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Heavy

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.3 � 3.4

characteristic: temperature

text: 151_Buckeye Trail Heavy Plot 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.5 � 0.7

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 58.3

characteristic: dissolved organic carbon

text: 550.0 � 75.0

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.2 � 3.6

text: 134_Englewood Reference Plot 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 550.0 � 75.0

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 19-J_Englewood Moderate Plot 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.8 � 0.6

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 16.7

characteristic: dissolved organic carbon

text: 550.0 � 75.0

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.2

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.8 � 2.7

text: 26-J_Charleston Falls Moderate Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.8 � 0.6

text: 116.6 � 16.7

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.2

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.8 � 2.7

characteristic: temperature

text: 87_Charleston Falls Moderate Plot 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.8 � 0.6

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 16.7

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.2

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.8 � 2.7

text: 55_Charleston Falls Moderate Plot 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.5 � 0.7

text: 116.6 � 58.3

text: 593.8 � 68.8

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Reference

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.2 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.2 � 3.6

characteristic: temperature

text: 10-J_Englewood Reference Plot 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 593.8 � 68.8

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 15-28d_Englewood Moderate Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 593.8 � 68.8

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 21-J_Englewood Moderate Plot 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.5 � 0.7

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 58.3

characteristic: dissolved organic carbon

text: 593.8 � 68.8

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.2 � 3.6

text: 11-J_Englewood Reference Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.5 � 0.7

text: 116.6 � 58.3

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Reference

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.2 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.2 � 3.6

characteristic: temperature

text: 131_Englewood Reference Plot 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 581.3 � 4.4

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 52_Englewood Moderate Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.9 � 0.1

text: 124.9 � 8.3

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Reference

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.2 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.4 � 2.0

characteristic: temperature

text: 76_Aullwood Reference Plot 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 5.5 � 0.7

characteristic: alkalinity

characteristic: collection date

text: 116.6 � 58.3

characteristic: dissolved organic carbon

text: 581.3 � 4.4

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.2 � 3.6

text: 8-J_Englewood Reference Plot 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.8 � 0.6

text: 116.6 � 16.7

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.2

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.8 � 2.7

characteristic: temperature

text: 16-28d_Charleston Falls Moderate Plot 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 1.0

characteristic: alkalinity

characteristic: collection date

text: 249.8 � 74.9

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Heavy

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.3 � 3.4

text: 62_Buckeye Trail Heavy Plot 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 12-28d_Englewood Moderate Plot 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 0.8

characteristic: alkalinity

characteristic: collection date

text: 224.8 � 133.2

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Moderate

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.9 � 4.2

text: 11-M_Englewood Moderate Plot 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 5.8 � 0.6

text: 116.6 � 16.7

text: 556.3 � 81.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.2

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.8 � 2.7

characteristic: temperature

text: 18-28d_Charleston Falls Moderate Plot 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.6 � 1.0

characteristic: alkalinity

characteristic: collection date

text: 249.8 � 74.9

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Heavy

text: biofilm metagenome

characteristic: organism

text: 8.3 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.3 � 3.4

text: 22-28d_Buckeye Trail Heavy Plot 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 581.3 � 56.3

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 15-M_Englewood Moderate Plot 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6.9 � 0.1

characteristic: alkalinity

characteristic: collection date

text: 124.9 � 8.3

characteristic: dissolved organic carbon

text: 556.3 � 81.3

characteristic: dissolved oxygen

text: temperate forest

characteristic: environment (biome)

text: freshwater stream

characteristic: environment (feature)

text: biofilm

characteristic: environment (material)

text: USA

text: mimarks-survey

text: microbial mat/biofilm

text: Reference

text: biofilm metagenome

characteristic: organism

text: 8.2 � 0.1

text: glass marbles

text: biofilm metagenome

text: Illumina MiSeq

text: 16S rRNA

text: 15.4 � 2.0

text: 7-M_Aullwood Reference Plot 2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Michigan State University

text: public

text: 6.6 � 0.8

text: 224.8 � 133.2

text: 581.3 � 4.4

text: temperate forest

text: freshwater stream

text: biofilm

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: Moderate

characteristic: miscellaneous parameter

text: biofilm metagenome

text: 8.3 � 0.1

characteristic: ph

characteristic: project name

text: glass marbles

characteristic: sample collection device or method

characteristic: sample volume or weight for DNA extraction

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: 15.9 � 4.2

characteristic: temperature

text: 13-28d_Englewood Moderate Plot 3