characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 5 m at the Nares Strait station 177

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITE LAVAL

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 5 m at the Nares Strait station 177

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 3 µm

characteristic: size fraction lower threshold

text: 50 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 5 m at the Nares Strait station 177

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Large fraction of the filtrated seawater sampled at 0 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 0 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 0.22 µm

characteristic: size fraction lower threshold

text: 3 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 0 m at the Nares Strait station 139

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 0 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Large fraction of the filtrated seawater sampled at 15 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 3 µm

characteristic: size fraction lower threshold

text: 50 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 15 m at the Nares Strait station 139

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 15 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 15 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 0.22 µm

characteristic: size fraction lower threshold

text: 3 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Large fraction of the filtrated seawater sampled at 562 m at the Nares Strait station 139

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 562 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 562 m at the Nares Strait station 139

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 3 µm

characteristic: size fraction lower threshold

text: 50 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 562 m at the Nares Strait station 139

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Large fraction of the filtrated seawater sampled at 0 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 0 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 0.22 µm

characteristic: size fraction lower threshold

text: 3 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 0 m at the Nares Strait station Kane1

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 0 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 236 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 0.22 µm

characteristic: size fraction lower threshold

text: 3 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 236 m at the Nares Strait station Kane1

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 236 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: DNA extracted from the Large fraction of the filtrated seawater sampled at 23 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 3 µm

characteristic: size fraction lower threshold

text: 50 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: DNA extracted from the Small fraction of the filtrated seawater sampled at 23 m at the Nares Strait station Kane1

characteristic: description

text: Ocean

characteristic: environment (biome)

text: Sea water

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

text: 0.22 µm

text: 3 µm


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE LAVAL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: uncultured eukaryote

text: RNA extracted from the Large fraction of the filtrated seawater sampled at 23 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

characteristic: environmental sample

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: 3 µm

text: 50 µm


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE LAVAL

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: RNA extracted from the Small fraction of the filtrated seawater sampled at 23 m at the Nares Strait station Kane1

text: Ocean

text: Sea water

text: Water

text: Yes

text: Arctic Ocean

text: uncultured eukaryote

text: uncultured eukaryote

text: 0.22 µm

characteristic: size fraction lower threshold

text: 3 µm

characteristic: size fraction upper threshold

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:South African Sugarcane Research Institute

text: Assembly of the chloroplast of Andropogon virginicus from whole genome Illumina data.

characteristic: description

text: United Kingdom

text: Cambridgeshire, Cambridge

characteristic: geographic location (region and locality)

text: Andropogon virginicus

text: Andropogon virginicus

text: leaf

characteristic: tissue type

text: Chloroplast assembly and annotation of Andropogon virginicus


characteristic: ENA-FIRST-PUBLIC

text: South African Sugarcane Research Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Mitochondiral DNA isolated from blood sample, illumina sequenced, assembled and annotated.

text: Buceros rhinoceros silvestris

characteristic: organism

text: Buceros rhinoceros silvestris

text: Mitochondrial assembly of Buceros rhinoceros silvestris


text: CNGB

text: public

text: CNSA

characteristic: broker name

characteristic: collection date

text: Human-associated

characteristic: env biome

text: Female reproductive tract

characteristic: env feature

text: cervical mucus

text: China: Shenzhen

characteristic: geo loc name

text: Homo sapiens

characteristic: host

text: metagenome

characteristic: investigation type

text: 39.55 N 116.22 W

characteristic: lat lon

text: human reproductive system metagenome

text: not collected

text: human reproductive system metagenome


Myzus cerasi clonal line1

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The James Hutton Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Myzus cerasi clonal line1

tag: Namespace:The James Hutton Institute

text: Whole body

characteristic: cell type

text: Peter Thorpe

characteristic: collected by

text: black cherry aphid

characteristic: common name

text: The james Hutton Institute clonal line

characteristic: culture collection

text: Genomic reads for black cherry aphid Myzus cerasi. 2X250bp and 2X150bp, PCR-free library preparation. Insert size aim was 500bp. Sequenced at TGAC UK.

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Dundee, JHI

text: Prunus avium

characteristic: host scientific name

text: growth rooms at 18 °C with a 16 h light and 8 h dark period

characteristic: isolation source

text: American Land Cress (Barbarea verna)

characteristic: lab host

text: asexual parthenogenesis

characteristic: mating type

text: Myzus cerasi

text: Myzus cerasi

text: Myzus cerasi genomic reads

characteristic: title


Myzus cerasi clonal line2

characteristic: ENA-FIRST-PUBLIC

text: The James Hutton Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Myzus cerasi clonal line2

characteristic: Submitter Id

text: Whole body

text: Peter Thorpe

text: black cherry aphid

text: The james Hutton Institute clonal line

characteristic: description

text: United Kingdom

text: Dundee, JHI

characteristic: geographic location (region and locality)

text: Prunus avium

text: American Land Cress (Barbarea verna)

text: asexual parthenogenesis

text: Myzus cerasi

characteristic: organism

text: Myzus cerasi

text: Myzus cerasi genomic reads


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Using 12 novel PCR primers, the complete sequence of Sorghum arundinaceum was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: Sorghum arundinaceum

text: Sorghum arundinaceum

text: leaf

characteristic: tissue type

text: Chloroplast assembly of Sorghum arundinaceum


LMG 28615

text: LM-UGent

text: public

text: LMG 28615

text: New Caledonia

characteristic: geographic location (country and/or sea)

text: LMG 28615T

characteristic: isolate

text: Plant roots

characteristic: isolation source

text: Burkholderia novacaledonica

text: Burkholderia novacaledonica

text: Burkholderia novacaledonica LMG 28615T


text: Liver

characteristic: ArrayExpress-PHENOTYPE

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Liver

characteristic: phenotype

text: Mus musculus

characteristic: subject id

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Using 12 novel PCR primers, the complete sequence of Sorghum halepense was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

characteristic: description

text: Sorghum halepense

characteristic: organism

text: Sorghum halepense

text: leaf

text: Chloroplast assembly of Sorghum halepense


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CNGB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: CNSA

characteristic: broker name

characteristic: collection date

text: Human-associated

characteristic: env biome

text: Female reproductive tract

characteristic: env feature

text: peritoneal fluid

text: China: Shenzhen

characteristic: geo loc name

text: Homo sapiens

characteristic: host

text: metagenome

characteristic: investigation type

text: 39.55 N 116.22 W

characteristic: lat lon

text: human reproductive system metagenome

text: not collected

text: human reproductive system metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Using 12 novel PCR primers, the complete sequence of Sorghum versicolor was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: Sorghum versicolor

text: Sorghum versicolor

text: leaf

characteristic: tissue type

text: Chloroplast assembly of Sorghum (Sarga) versicolor

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Sudan grass

characteristic: common name

text: Using 12 novel PCR primers, the complete sequence of Sorghum x drummondii was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

characteristic: description

text: Sorghum x drummondii

characteristic: organism

text: Sorghum x drummondii

text: leaf

text: Chloroplast assembly of Sorghum x drummondii


text: CNGB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: CNSA

characteristic: broker name

characteristic: collection date

text: Human-associated

characteristic: env biome

text: Female reproductive tract

characteristic: env feature

text: posterior fornix

text: China: Shenzhen

characteristic: geo loc name

text: Homo sapiens

text: metagenome

text: 39.55 N 116.22 W

text: human reproductive system metagenome

text: not collected

text: human reproductive system metagenome


text: SCC

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Female

characteristic: gender

text: Mus musculus

text: SCC

characteristic: phenotype

text: Mus musculus

characteristic: subject id


text: Papillomas

text: female

text: Mus musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Female

text: Mus musculus

characteristic: organism

text: Papillomas

text: Mus musculus

characteristic: title


text: Tail

characteristic: ArrayExpress-PHENOTYPE

text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

text: SC

characteristic: INSDC last update

text: public

text: house mouse

text: Female

text: Mus musculus

text: Tail

text: Mus musculus


text: SCC

text: male

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Male

text: Mus musculus

text: SCC

text: Mus musculus


text: Skin

text: female

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Female

characteristic: gender

text: Mus musculus

text: Skin

text: Mus musculus


text: Skin

text: male

text: Mus musculus

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Male

text: Mus musculus

text: Skin

characteristic: phenotype

text: Mus musculus

characteristic: subject id


text: Tail

text: male

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: house mouse

text: Male

text: Mus musculus

text: Tail

text: Mus musculus


text: CNGB

text: public

characteristic: Submitter Id

text: CNSA

characteristic: broker name

characteristic: collection date

text: Human-associated

characteristic: env biome

text: Female reproductive tract

characteristic: env feature

text: cervical mucus

text: China: Shenzhen

characteristic: geo loc name

text: Homo sapiens

characteristic: host

text: metagenome

characteristic: investigation type

text: 39.55 N 116.22 W

characteristic: lat lon

text: human reproductive system metagenome

characteristic: organism

text: not collected

text: human reproductive system metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CNGB

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: CNSA

text: Human-associated

text: Female reproductive tract

text: endometrium

text: China: Shenzhen

text: Homo sapiens

text: metagenome

text: 39.55 N 116.22 W

text: human reproductive system metagenome

text: not collected

text: human reproductive system metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CNGB

characteristic: INSDC center name

text: public

text: CNSA

text: Human-associated

text: Female reproductive tract

text: lower third of vagina

text: China: Shenzhen

text: Homo sapiens

text: metagenome

text: 39.55 N 116.22 W

text: human reproductive system metagenome

text: not collected

text: human reproductive system metagenome


text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

characteristic: subject id

characteristic: title


text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

text: SC

text: public

text: house mouse

text: Female

characteristic: gender

text: Mus musculus

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: house mouse

text: Male

text: Mus musculus

characteristic: organism

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Institute of Soil Science and Plant Cultivation State Research Institute

characteristic: collection date

text: Direct sowing - Before sowing

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: agricultural soil

characteristic: environment (feature)

text: bulk soil

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 51.23 N

characteristic: geographic location (latitude)

text: 21.38 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: Fungal genetics and functional diversity of microbial communities in the soil under long-term monoculture of maize using different cultivation techniques

characteristic: project name

text: metagenome

text: illumina, miseq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

type: has member


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Soil Science and Plant Cultivation State Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Reduced tillage - Before sowing

text: agricultural soil

text: agricultural soil

text: bulk soil

text: Poland

text: 51.23 N

text: 21.38 E

text: metagenome

text: metagenome

text: metagenome

text: illumina, miseq

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: collection date

text: Full tillage - Before sowing

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: agricultural soil

characteristic: environment (feature)

text: bulk soil

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 51.23 N

text: 21.38 E

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: illumina, miseq

text: soil


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Crop rotation - Before sowing

text: agricultural soil

text: agricultural soil

text: bulk soil

text: Poland

text: 51.23 N

characteristic: geographic location (latitude)

text: 21.38 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: illumina, miseq

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Direct sowing - Flowering

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: agricultural soil

text: bulk soil

text: Poland

text: 51.23 N

text: 21.38 E

text: metagenome

text: metagenome

text: metagenome

text: illumina, miseq

text: soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Reduced tillage - Flowering

text: agricultural soil

text: agricultural soil

characteristic: environment (feature)

text: bulk soil

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 51.23 N

text: 21.38 E

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: illumina, miseq

text: soil


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Full tillage - Flowering

text: agricultural soil

text: agricultural soil

text: bulk soil

text: Poland

text: 51.23 N

characteristic: geographic location (latitude)

text: 21.38 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: illumina, miseq

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Crop rotation - Flowering

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: agricultural soil

text: bulk soil

text: Poland

text: 51.23 N

text: 21.38 E

text: metagenome

text: metagenome

text: metagenome

text: illumina, miseq

text: soil

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Utah

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:University of Utah

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Whole cerebellar tissue was extracted from WT and transgenic (ATXN2Q127) mice that were age matched for each experiment. Mice were anesthetized using isoflurane and sacrificed. The cerebellum was quickly dissected and placed in ice-cold extracellular solution (119 mM NaCl, 26 mM NaHCO3, 11 mM glucose, 2.5 mM KCl, 2.5 mM CaCl2, 1.3 mM MgCl2 and 1 mM NaH2PO4, pH 7.4 when gassed with 5% CO2 and 95% O2). Total RNA was isolated using miRNeasy Mini Kit (Qiagen Inc., USA) according to the manufacturer’s protocol. Illumina TrueSeq Stranded Total RNA Sample Prep with Ribo-Zero rRNA Removal Kit for mouse

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: cerebellum

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Utah

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Spinocerebellar ataxia type 2

text: Mus musculus

text: Mus musculus

text: cerebellum

text: Mus musculus

text: male


characteristic: ENA-CHECKLIST

text: University of Utah

characteristic: INSDC first public

characteristic: INSDC last update

text: public

unit: day

text: ArrayExpress

text: house mouse

characteristic: description

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: cerebellum

text: Mus musculus

text: male

characteristic: title


text: University of Utah

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Spinocerebellar ataxia type 2

characteristic: disease

text: Mus musculus

text: Mus musculus

text: cerebellum

text: Mus musculus

text: male


text: OX

text: public

text: Escherichia coli

characteristic: organism

text: Escherichia coli


characteristic: ENA-FIRST-PUBLIC

text: OX

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-LAST-UPDATE

text: OX

text: public

text: Citrobacter freundii

text: Citrobacter freundii


characteristic: External Id

text: OX

text: public

text: Klebsiella oxytoca

text: Klebsiella oxytoca


text: OX

characteristic: INSDC center name

text: public

text: Enterobacter hormaechei

text: Enterobacter hormaechei


text: OX

text: public

characteristic: SRA accession

text: Enterobacter cloacae

text: Enterobacter cloacae


text: OX

text: public

characteristic: Submitter Id

text: unidentified

text: unidentified


characteristic: ENA-CHECKLIST

text: OX

text: public

text: Klebsiella aerogenes

text: Klebsiella aerogenes


text: OX

characteristic: INSDC first public

text: public

text: Raoultella ornithinolytica

text: Raoultella ornithinolytica


text: OX

characteristic: INSDC last update

text: public

text: Citrobacter pasteurii

text: Citrobacter pasteurii


text: OX

text: public

characteristic: description

text: Acinetobacter baumannii

text: Acinetobacter baumannii


text: OX

text: public

text: Klebsiella variicola

text: Klebsiella variicola

characteristic: title


text: OX

text: public

characteristic: INSDC status

text: Pluralibacter gergoviae

text: Pluralibacter gergoviae


text: OX

text: public

text: Klebsiella michiganensis

text: Klebsiella michiganensis


text: OX

text: public

text: Enterobacter muelleri

text: Enterobacter muelleri


text: OX

text: public

text: Hafnia alvei

text: Hafnia alvei


text: OX

text: public

text: Citrobacter sedlakii

text: Citrobacter sedlakii


text: OX

text: public

text: Escherichia coli

text: Escherichia coli


text: OX

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: OX

text: public

text: Citrobacter freundii

text: Citrobacter freundii


text: OX

text: public

text: Chlamydia trachomatis

text: Chlamydia trachomatis


text: OX

text: public

text: Klebsiella oxytoca

text: Klebsiella oxytoca


text: OX

text: public

text: Enterobacter cloacae

text: Enterobacter cloacae


text: ED

text: public

text: F1 hybrid; G. rivale x G. urbanum

text: Located 11 km southwest of Edinburgh at an elevation of 150 m. It extends for 400 m along the southern bank of the Water of Leith occupying semi-natural woodland vegetation beside a railway track that was abandoned in the 1960s

characteristic: geographic location (region and locality)

text: 55 degrees 53 minutes N/3 degrees 20 minutes W

characteristic: lat lon

text: Geum rivale

text: Geum rivale


text: ED

text: public

text: Geum urbanum

text: Geum urbanum

text: Geum urbanum


text: ED

text: public

text: Geum rivale

text: Geum rivale

text: Geum rivale


text: ED

text: public

text: Back-cross to G. rivale

text: Geum rivale

text: Geum rivale


name: Aspergillus niger N402 (ATCC 64974) goxB21

text: WAGENINGEN UNIVERSITY

text: public

tag: Namespace:WAGENINGEN UNIVERSITY

text: Aspergillus niger

text: Aspergillus niger

text: N402 (ATCC 64974)

characteristic: strain

characteristic: sub strain


E-MTAB-46981515497013:21 Tumor

text: University of Michigan

text: public

text: E-MTAB-46981515497013:21 Tumor

tag: Namespace:University of Michigan

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Tissue was collected from subjects recruited through the Molecular Pathology and Biosample Core (MPBC) as part of the GI SPORE at the University of Michigan. Colonic tissue was acquired by endoscopy (adenomas) or from colonic resections (adenocarcinomas) and according to protocols approved by the University of Michigan Institutional Review Board (IRB; HUM00064405/0038437/00030020). Two growth media were used; the first for continuous culture and the second to drive to a cyst morphology. The first reduced medium (KGM-Gold) is a serum-free epithelial medium containing epidermal growth factor (EGF) and pituitary extract (KGMG; Lonza; 195769) with the antimicrobials 25µg/mL gentamicin (Invitrogen; 15750) and 50µg/mL Primocin (InvivoGen; ant-pm-1) and made to 0.15mM calcium with CaCl2 (PromCell). The second (L-WRN medium) contains high levels of Wnt3a, R-spondin-3 and Noggin provided by a conditioned medium from support L- cells as described by Miyoshi 3. The complete L-WRN medium further contained Advanced DMEM/F-12, 2mM GlutaMax, 10mM HEPES, N-2 media supplement, B-27 supplement minus vitamin A, 100 units/mL penicillin, 0.1 mg/mL streptomycin (all Invitrogen), 50µg/ml Primocin, 1mM N-Acetyl-L-cysteine (Sigma; A9165), and 100ng EGF/mL (R&D; 236-EG), as well as the addition of 10mM Nicotinamide (Sigma; N0636). To drive from a budding to cyst morphology, cultures are gradually switched from KGMG to L-WRN for three to four weeks (3 days KGMG, 5 days 50/50 L-WRN; 5 days 75/25; 100% L-WRN until predominately cystic). Consistent with previous reports, organoids formed cystic structures in the presence of Wnt ligand provided by L-WRN medium 4-8. Cultures were propagated in Matrigel (Corning; 354234) which was made to 8mg/mL in growth media on 6-well tissue culture plates (USA Scientific CytoOne; CC7682-7506). All plasticware, excluding culture plates, were coated in 0.1% bovine serum albumen in DPBS (Sigma; A8806) to prevent tissue/organoids/cells from adhering to surfaces. Cultures were passaged every 4-7 days by digesting Matrigel in cold 2mM EDTA in DPBS and plated the first day with the Rho-associated protein kinase (ROCK) inhibitor, Y27632 (10µM; Miltenyi; 130-104-169). On the day of sorting, the cultures were treated with 10µM Y27632 for 2.5 hrs prior to harvest. Matrigel was digested for 45 minutes with cold 2mM EDTA in DPBS and organoids were washed 3X with cold DPBS (100xg at 4C). Structures were dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec; 130-095-929) with the protocol modifications described as follows. The enzymes were prepared in warm HBSS modified to 0.13mM calcium and 0.9mM magnesium [10% HBSS, calcium, magnesium, no phenol red (Life Technologies 14025092) with 90% HBSS, no calcium, no magnesium (Life Technologies 14170112)] to minimize differentiation of the epithelial cells while supporting enzymatic activity. The organoids were suspended in 20mL of enzyme solution containing 5µM Y27632, then transferred to two BSA-coated C-tubes (Miltenyi Biotec; 130-096-334) and dissociated with a gentleMACS Dissociator (Miltenyi; 130-093-235) using the program h_tumor_01, once at the onset and every 15 minutes for 1 hour, and finishing with two program runs. Between runs the suspension was slowly rotated at 37C. The cell suspension was then poured over a succession of cold BSA-coated cell strainers into 0.5% BSA-2mM EDTA-PBS: 100µm (Corning; DL 352360) to 40µm (Corning; DL 352340) to 20µm (CellTrics; 04-0042-2315). The cells were washed 3X in the 0.5% BSA-2mM EDTA-PBS and centrifuged for 5 minutes at 500xg. Cells were counted under trypan blue exclusion to estimate live cell number using a Countess Automated Cell Counter (Invitrogen; C10227). DNA was extracted from whole tissue or organoid cultures using the DNeasy Mini Kit (Qiagen). DNA concentration and quality were determined using a Nanodrop (Thermo Scientific) and Bioanalyzer (Agilent). Targeted panel for colorectal cancer (Qiagen, #DHS-002Z). Targets 71 genes.

text: colon adenocarcinoma

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 21 Tumor


E-MTAB-46981515497013:21 Normal

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-46981515497013:21 Normal

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 21 Normal


E-MTAB-46981515497013:21 3m LWRN

text: University of Michigan

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-46981515497013:21 3m LWRN

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: colon adenocarcinoma

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 21 3m LWRN

characteristic: title


E-MTAB-46981515497013:236 Tumor

text: University of Michigan

text: public

text: E-MTAB-46981515497013:236 Tumor

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: adenomatous colon polyp

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 236 Tumor


E-MTAB-46981515497013:236 Normal

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:236 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 236 Normal


E-MTAB-46981515497013:236 3.7m LWRN

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

characteristic: INSDC center name

text: public

text: E-MTAB-46981515497013:236 3.7m LWRN

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 236 3.7m LWRN


E-MTAB-46981515497013:245 Tumor

text: University of Michigan

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-46981515497013:245 Tumor

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

text: Colon Sessile Serrated Adenoma/Polyp

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 245 Tumor


E-MTAB-46981515497013:245 Normal

text: University of Michigan

text: public

characteristic: INSDC status

text: E-MTAB-46981515497013:245 Normal

text: ArrayExpress

text: tissue

text: human

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 245 Normal

characteristic: title


E-MTAB-46981515497013:245 2m LWRN

text: University of Michigan

text: public

text: E-MTAB-46981515497013:245 2m LWRN

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Colon Sessile Serrated Adenoma/Polyp

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 245 2m LWRN


E-MTAB-46981515497013:282 Tumor

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:282 Tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 282 Tumor


E-MTAB-46981515497013:282 Normal

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:282 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 282 Normal


E-MTAB-46981515497013:282 4m KGM

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-46981515497013:282 4m KGM

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; KGM medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 282 4m KGM


E-MTAB-46981515497013:300 Tumor

text: University of Michigan

text: public

characteristic: INSDC status

text: E-MTAB-46981515497013:300 Tumor

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

characteristic: description

text: adenomatous colon polyp

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 300 Tumor

characteristic: title


E-MTAB-46981515497013:300 Normal

text: University of Michigan

text: public

text: E-MTAB-46981515497013:300 Normal

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 300 Normal


E-MTAB-46981515497013:300 8m KGM

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:300 8m KGM

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

characteristic: disease

text: organoid culture; KGM medium

characteristic: growth condition

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 300 8m KGM


E-MTAB-46981515497013:469 Tumor

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:469 Tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 469 Tumor


E-MTAB-46981515497013:469 Normal

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:469 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 469 Normal


E-MTAB-46981515497013:469 4m LWRN

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:469 4m LWRN

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 469 4m LWRN


E-MTAB-46981515497013:574 tumor

text: University of Michigan

text: public

text: E-MTAB-46981515497013:574 tumor

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: adenomatous colon polyp

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 574 tumor

characteristic: title


E-MTAB-46981515497013:574 5.7m

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:574 5.7m

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

characteristic: disease

text: organoid culture; KGM medium

characteristic: growth condition

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 574 5.7m


E-MTAB-46981515497013:575 Tumor

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:575 Tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 575 Tumor


E-MTAB-46981515497013:575 Normal

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:575 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 575 Normal


E-MTAB-46981515497013:575 5.5m KGM

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:575 5.5m KGM

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: adenomatous colon polyp

text: organoid culture; KGM medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 575 5.5m KGM


E-MTAB-46981515497013:575 5m LWRN

text: University of Michigan

text: public

text: E-MTAB-46981515497013:575 5m LWRN

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 575 5m LWRN

characteristic: title


E-MTAB-46981515497013:584 tumor

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:584 tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 584 tumor


E-MTAB-46981515497013:584 4m

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:584 4m

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; KGM medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 584 4m


E-MTAB-46981515497013:590 1.5y KGM

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:590 1.5y KGM

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; KGM medium

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 590 1.5y KGM


E-MTAB-46981515497013:590 1.5y LWRN

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:590 1.5y LWRN

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 590 1.5y LWRN


E-MTAB-46981515497013:610 Tumor

text: University of Michigan

text: public

text: E-MTAB-46981515497013:610 Tumor

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adenomatous colon polyp

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 610 Tumor


E-MTAB-46981515497013:610 Normal

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:610 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 610 Normal

characteristic: title


E-MTAB-46981515497013:610 4m LWRN

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:610 4m LWRN

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 610 4m LWRN


E-MTAB-46981515497013:664 Tumor

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:664 Tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 664 Tumor


E-MTAB-46981515497013:664 Normal

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:664 Normal

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 664 Normal


E-MTAB-46981515497013:664 1.5m LWRN

text: University of Michigan

text: public

text: E-MTAB-46981515497013:664 1.5m LWRN

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 664 1.5m LWRN


E-MTAB-46981515497013:708 Tumor

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:708 Tumor

text: ArrayExpress

text: tissue

text: human

text: Colon Sessile Serrated Adenoma/Polyp

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 708 Tumor

characteristic: title


E-MTAB-46981515497013:708 Normal

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:708 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 708 Normal


E-MTAB-46981515497013:708 0.5m LWRN

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:708 0.5m LWRN

text: ArrayExpress

text: epithelial cell

text: human

text: Colon Sessile Serrated Adenoma/Polyp

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 708 0.5m LWRN


E-MTAB-46981515497013:726 Tumor

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:726 Tumor

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 726 Tumor


E-MTAB-46981515497013:726 Normal

text: University of Michigan

text: public

text: E-MTAB-46981515497013:726 Normal

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 726 Normal


E-MTAB-46981515497013:726 2m LWRN

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:726 2m LWRN

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: adenomatous colon polyp

characteristic: disease

text: organoid culture; LWRN medium

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 726 2m LWRN


E-MTAB-46981515497013:735 Tumor

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:735 Tumor

text: ArrayExpress

text: tissue

text: human

text: adenomatous colon polyp

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 735 Tumor

characteristic: title


E-MTAB-46981515497013:735 Normal

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:735 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 735 Normal


E-MTAB-46981515497013:735 3m KGM

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:735 3m KGM

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; KGM medium

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 735 3m KGM


E-MTAB-46981515497013:735 2m LWRN

text: University of Michigan

text: public

text: E-MTAB-46981515497013:735 2m LWRN

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 735 2m LWRN


E-MTAB-46981515497013:772 Tumor

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:772 Tumor

text: ArrayExpress

text: tissue

text: human

characteristic: description

text: adenomatous colon polyp

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 772 Tumor


E-MTAB-46981515497013:772 Normal

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: E-MTAB-46981515497013:772 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 772 Normal


E-MTAB-46981515497013:772 1.5m LWRN

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-46981515497013:772 1.5m LWRN

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 772 1.5m LWRN

characteristic: title


E-MTAB-46981515497013:781 Tumor

text: University of Michigan

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:781 Tumor

characteristic: Submitter Id

text: ArrayExpress

text: tissue

text: human

text: colon adenocarcinoma

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 781 Tumor


E-MTAB-46981515497013:781 Normal

text: University of Michigan

text: public

text: E-MTAB-46981515497013:781 Normal

text: ArrayExpress

characteristic: broker name

text: tissue

characteristic: cell type

text: human

characteristic: common name

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 781 Normal


E-MTAB-46981515497013:781 1.5m KGM

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:781 1.5m KGM

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: colon adenocarcinoma

text: organoid culture; KGM medium

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 781 1.5m KGM


E-MTAB-46981515497013:781 1m LWRN

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Michigan

text: public

text: E-MTAB-46981515497013:781 1m LWRN

text: ArrayExpress

text: epithelial cell

text: human

text: colon adenocarcinoma

characteristic: disease

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 781 1m LWRN


E-MTAB-46981515497013:815 Tumor

characteristic: External Id

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-46981515497013:815 Tumor

text: ArrayExpress

text: tissue

text: human

text: colon adenocarcinoma

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 815 Tumor


E-MTAB-46981515497013:815 Normal

text: University of Michigan

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:815 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 815 Normal

characteristic: title


E-MTAB-46981515497013:815 1.5m KGM

text: University of Michigan

text: public

text: E-MTAB-46981515497013:815 1.5m KGM

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: colon adenocarcinoma

text: organoid culture; KGM medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 815 1.5m KGM


E-MTAB-46981515497013:815 0.5m LWRN

characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: E-MTAB-46981515497013:815 0.5m LWRN

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: colon adenocarcinoma

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: Homo sapiens

text: 815 0.5m LWRN


E-MTAB-46981515497013:861 Tumor

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Michigan

text: public

text: E-MTAB-46981515497013:861 Tumor

text: ArrayExpress

text: tissue

text: human

text: colon adenocarcinoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: Homo sapiens

text: 861 Tumor


E-MTAB-46981515497013:861 Normal

characteristic: External Id

text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-46981515497013:861 Normal

text: ArrayExpress

text: tissue

text: human

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 861 Normal


E-MTAB-46981515497013:861 1.5m KGM

text: University of Michigan

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-46981515497013:861 1.5m KGM

text: ArrayExpress

text: epithelial cell

text: human

text: colon adenocarcinoma

text: organoid culture; KGM medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 861 1.5m KGM

characteristic: title


E-MTAB-46981515497013:861 0.5m LWRN

text: University of Michigan

text: public

text: E-MTAB-46981515497013:861 0.5m LWRN

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: colon adenocarcinoma

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens

text: 861 0.5m LWRN


characteristic: ENA-CHECKLIST

text: University of Michigan

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: Tissue was collected from subjects recruited through the Molecular Pathology and Biosample Core (MPBC) as part of the GI SPORE at the University of Michigan. Colonic tissue was acquired by endoscopy (adenomas) or from colonic resections (adenocarcinomas) and according to protocols approved by the University of Michigan Institutional Review Board (IRB; HUM00064405/0038437/00030020). Two growth media were used; the first for continuous culture and the second to drive to a cyst morphology. The first reduced medium (KGM-Gold) is a serum-free epithelial medium containing epidermal growth factor (EGF) and pituitary extract (KGMG; Lonza; 195769) with the antimicrobials 25µg/mL gentamicin (Invitrogen; 15750) and 50µg/mL Primocin (InvivoGen; ant-pm-1) and made to 0.15mM calcium with CaCl2 (PromCell). The second (L-WRN medium) contains high levels of Wnt3a, R-spondin-3 and Noggin provided by a conditioned medium from support L- cells as described by Miyoshi 3. The complete L-WRN medium further contained Advanced DMEM/F-12, 2mM GlutaMax, 10mM HEPES, N-2 media supplement, B-27 supplement minus vitamin A, 100 units/mL penicillin, 0.1 mg/mL streptomycin (all Invitrogen), 50µg/ml Primocin, 1mM N-Acetyl-L-cysteine (Sigma; A9165), and 100ng EGF/mL (R&D; 236-EG), as well as the addition of 10mM Nicotinamide (Sigma; N0636). To drive from a budding to cyst morphology, cultures are gradually switched from KGMG to L-WRN for three to four weeks (3 days KGMG, 5 days 50/50 L-WRN; 5 days 75/25; 100% L-WRN until predominately cystic). Consistent with previous reports, organoids formed cystic structures in the presence of Wnt ligand provided by L-WRN medium 4-8. Cultures were propagated in Matrigel (Corning; 354234) which was made to 8mg/mL in growth media on 6-well tissue culture plates (USA Scientific CytoOne; CC7682-7506). All plasticware, excluding culture plates, were coated in 0.1% bovine serum albumen in DPBS (Sigma; A8806) to prevent tissue/organoids/cells from adhering to surfaces. Cultures were passaged every 4-7 days by digesting Matrigel in cold 2mM EDTA in DPBS and plated the first day with the Rho-associated protein kinase (ROCK) inhibitor, Y27632 (10µM; Miltenyi; 130-104-169). On the day of sorting, the cultures were treated with 10µM Y27632 for 2.5 hrs prior to harvest. Matrigel was digested for 45 minutes with cold 2mM EDTA in DPBS and organoids were washed 3X with cold DPBS (100xg at 4C). Structures were dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec; 130-095-929) with the protocol modifications described as follows. The enzymes were prepared in warm HBSS modified to 0.13mM calcium and 0.9mM magnesium [10% HBSS, calcium, magnesium, no phenol red (Life Technologies 14025092) with 90% HBSS, no calcium, no magnesium (Life Technologies 14170112)] to minimize differentiation of the epithelial cells while supporting enzymatic activity. The organoids were suspended in 20mL of enzyme solution containing 5µM Y27632, then transferred to two BSA-coated C-tubes (Miltenyi Biotec; 130-096-334) and dissociated with a gentleMACS Dissociator (Miltenyi; 130-093-235) using the program h_tumor_01, once at the onset and every 15 minutes for 1 hour, and finishing with two program runs. Between runs the suspension was slowly rotated at 37C. The cell suspension was then poured over a succession of cold BSA-coated cell strainers into 0.5% BSA-2mM EDTA-PBS: 100µm (Corning; DL 352360) to 40µm (Corning; DL 352340) to 20µm (CellTrics; 04-0042-2315). The cells were washed 3X in the 0.5% BSA-2mM EDTA-PBS and centrifuged for 5 minutes at 500xg. Cells were counted under trypan blue exclusion to estimate live cell number using a Countess Automated Cell Counter (Invitrogen; C10227). RNA was extracted from sorted cells using the RNeasy Micro Kit (Qiagen; 74004) with on column DNase digestion. RNA concentration and quality were determined using a Nanodrop (Thermo Scientific) and Bioanalyzer (Agilent). ). Due to the small number of cells following FACS sorting and corresponding low level of input RNA, we depleted ribosomal RNAs with RiboGone (Clontech; 634846) and prepared sequencing libraries utilizing the SMARTer Stranded RNA-Seq kit (Clontech) following the manufacturer’s recommended protocol.

characteristic: description

text: adenomatous colon polyp

text: organoid culture; LWRN medium

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: FTN (Flow Through LGR5 Negative)

characteristic: phenotype

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Michigan

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

characteristic: disease

text: organoid culture; LWRN medium

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: SortedNeg (Magnet bound, LGR5 negative by FACS)

text: Homo sapiens


text: University of Michigan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: epithelial cell

text: human

text: adenomatous colon polyp

text: organoid culture; LWRN medium

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

text: SortedPos (Magnet bound, LGR5 positive by FACS)

text: Homo sapiens


Lactobacillus rhamnosus

text: public

characteristic: SRA accession

text: Lactobacillus rhamnosus

tag: Namespace:Institute of Biotechnology, University of Helsinki

characteristic: Submitter Id

text: metagenome-assembled Lactobacillus rhamnosus genome from Maasdam Cheese with high percentage completeness.

text: Lactobacillus rhamnosus

text: Lactobacillus rhamnosus

text: Lactobacillus rhamnosus

characteristic: title


text: Mouse

characteristic: ArrayExpress-PHENOTYPE

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mouse

text: Mus musculus

text: 36.8 PLKX

characteristic: subject id


text: Mouse

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Mouse

characteristic: phenotype

text: Mus musculus

text: 25.2 PLKX


text: Mouse

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Mouse

text: Mus musculus

text: 35.3 PLKX


text: CHINESE ACADEMY OF SCIENCES

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:CHINESE ACADEMY OF SCIENCES

characteristic: collection date

text: ureC

characteristic: description

text: agricuture

characteristic: environment (biome)

text: winter wheat-summer maize

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil

characteristic: project name

text: soil metagenome

text: illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: long-term nitrogen fertilization


E-MTAB-6336:KO 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6336:KO 1

tag: Namespace:Division of Computational Biology, University of Dundee

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Slc7a5 fl/fl mice harbouring two copies of the Slc7a5 targeted allele (exon 1 of Slc7a5 flanked with two loxP sites), were crossed with a mouse line ubiquitously expressing cre recombinase under the Bal1 promoter (Bal1-cre) to obtain a global Slc7a5 knockout mouse line (Poncet et al, 2014). Heterozygous Slc7a5+/- C57Bl/6 mice were viable and fertile. However, Slc7a5+/- inter-crosses did not produce any Slc7a5-null live mice. Here, heterozygous Slc7a5+/- mice were crossed to generate wild-type and Slc7a5-null embryos isolated at stage E8.5. Mouse colonies were breed, mice sacrificed and embryos isolated following Home Office guidelines (PPL 60/04454 or PPL 60/3455 and/or 60/4118). E8.5 embryos with 8 to 10 somites were dissected in cold PBS and lysed individually with Trizol reagent. Embryos were stage matched with respect to somite number and only male embryos were used to avoid potential sex-related variations in transcriptome. Allantois was kept for genotyping and sexing the embryos by PCR (McFarlane et al, 2013). Total RNA was extracted using the RNeasy mini kit (Qiagen). Samples were DNase treated ‘on-column’ using RNase-free DNase (Qiagen). Quality and quantity of total RNA were measured using a Qubit fluorometer (Invitrogen). Following quality control, 5 wild type and 5 Slc7a5-null male embryos were selected as biological replicates. Samples started with 300ng total RNA per embryo to which spike-ins (ERCC, 2ul of 1:100 dilution) were added. We used a TruSeq RNA v2 kit (Illumina RS-122-2001) to prepare the cDNA library following manufacturer’s instructions; polyA RNA selection was performed and fragment and random priming followed by cDNA synthesis, ligation and x10 rounds of PCR amplification.

text: embryonic day 8.25

characteristic: developmental stage

text: Bal1-cre / Slc7a5 flox/flox

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: whole organism

characteristic: organism part

text: Slc7a5 knockout

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: KO 1

characteristic: title


E-MTAB-6336:KO 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Division of Computational Biology, University of Dundee

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6336:KO 2

text: ArrayExpress

text: house mouse

characteristic: description

text: embryonic day 8.25

text: Bal1-cre / Slc7a5 flox/flox

text: Mus musculus

text: Mus musculus

text: whole organism

text: Slc7a5 knockout

text: Mus musculus

text: male

text: KO 2


E-MTAB-6336:KO 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6336:KO 3

text: ArrayExpress

text: house mouse

text: embryonic day 8.25

text: Bal1-cre / Slc7a5 flox/flox

text: Mus musculus

text: Mus musculus

text: whole organism

text: Slc7a5 knockout

text: Mus musculus

text: male

text: KO 3


E-MTAB-6336:KO 4

text: public

text: E-MTAB-6336:KO 4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: embryonic day 8.25

characteristic: developmental stage

text: Bal1-cre / Slc7a5 flox/flox

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: whole organism

text: Slc7a5 knockout

text: Mus musculus

text: male

text: KO 4


E-MTAB-6336:KO 5

text: public

text: E-MTAB-6336:KO 5

text: ArrayExpress

text: house mouse

text: embryonic day 8.25

text: Bal1-cre / Slc7a5 flox/flox

text: Mus musculus

characteristic: organism

text: Mus musculus

text: whole organism

characteristic: organism part

text: Slc7a5 knockout

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: KO 5


E-MTAB-6336:WT 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6336:WT 1

text: ArrayExpress

text: house mouse

text: embryonic day 8.25

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: whole organism

text: wild type

text: Mus musculus

text: male

text: WT 1

characteristic: title


E-MTAB-6336:WT 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-6336:WT 2

text: ArrayExpress

text: house mouse

characteristic: description

text: embryonic day 8.25

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: whole organism

text: wild type

text: Mus musculus

text: male

text: WT 2


E-MTAB-6336:WT 3

text: public

characteristic: SRA accession

text: E-MTAB-6336:WT 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: embryonic day 8.25

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: whole organism

text: wild type

text: Mus musculus

text: male

text: WT 3


E-MTAB-6336:WT 4

text: public

text: E-MTAB-6336:WT 4

text: ArrayExpress

text: house mouse

text: embryonic day 8.25

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: whole organism

characteristic: organism part

text: wild type

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: WT 4


E-MTAB-6336:WT 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6336:WT 5

text: ArrayExpress

text: house mouse

text: embryonic day 8.25

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: whole organism

text: wild type

text: Mus musculus

text: male

characteristic: strain

text: WT 5


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Shailesh Vinay Joshi, South African Sugarcane Research Institute

characteristic: collected by

characteristic: collection date

characteristic: cultivar

text: Using 12 novel PCR primers, the complete sequence of Saccharum spontaneum cultivar IM76-238 was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: South Africa

characteristic: geographic location (country and/or sea)

text: KwaZulu-Natal, Mount Edgecombe

characteristic: geographic location (region and locality)

text: Dyfed Lloyd Evans

characteristic: identified by

text: Saccharum spontaneum

text: Saccharum spontaneum

text: leaf

characteristic: tissue type

text: Chloroplast isolation, sequencing and assembly of Saccharum spontaneum cultivar IM76-238

characteristic: title


Lactobacillus helveticus

characteristic: ENA-CHECKLIST

text: Institute of Biotechnology, University of Helsinki

text: public

characteristic: SRA accession

text: Lactobacillus helveticus

characteristic: Submitter Id

text: metagenome-assembled Lactobacillus helveticus genome from Maasdam Cheese with high percentage completeness.

characteristic: description

text: Lactobacillus helveticus

characteristic: organism

text: Lactobacillus helveticus

text: Lactobacillus helveticus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: South African Sugarcane Research Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Mandalay

text: Using 12 novel PCR primers, the complete sequence of Saccharum spontaneum cultivar Mandalay was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: South Africa

text: KwaZulu-Natal, Mount Edgecombe

text: Dyfed Lloyd Evans

text: Saccharum spontaneum

text: Saccharum spontaneum

text: leaf

text: Chloroplast isolation, sequencing and assembly of Saccharum spontaneum cultivar Mandalay


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY

text: Transcriptome of Parapristionchus

text: Parapristionchus giblindavisi

text: Parapristionchus giblindavisi


text: public

characteristic: collected by

characteristic: collection date

text: Using 12 novel PCR primers, the complete sequence of Saccharum spontaneum cultivar Pasoeroen was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: South Africa

characteristic: geographic location (country and/or sea)

text: KwaZulu-Natal, South Africa

text: Saccharum spontaneum

text: Saccharum spontaneum

text: leaf

text: Chloroplast isolation, sequencing and assembly of Saccharum spontaneum cultivar Pasoeroen


text: MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY

text: public

text: Transccriptome of Pristionchus lucani

text: Pristionchus lucani

text: Pristionchus lucani


text: public

text: Transccriptome of Pristionchus atlanticus

text: Pristionchus atlanticus

text: Pristionchus atlanticus


text: public

tag: Namespace:Institute of Infection, Immunity and Inflammation

text: macrophages/monocytes

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: dev stage

text: Mus musculus

characteristic: host scientific name

text: Mus musculus

text: Mus musculus

characteristic: strain

text: Skin

characteristic: tissue type

text: ACKR2 and macrophage development


characteristic: ENA-CHECKLIST

text: Institute of Infection, Immunity and Inflammation

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: macrophages/monocytes

text: house mouse

text: Mus musculus

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Skin

text: ACKR2 and macrophage development

characteristic: title


text: Plasmodium falciparum 3D7

characteristic: ArrayExpress-SPECIES

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7

characteristic: subject id


text: Liverpool School of Tropical Medicine

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:Liverpool School of Tropical Medicine

text: 1 to 2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: cotton bollworm

characteristic: common name

text: Protocols: Samples snap frozen in liquid nitrogen and stored at -80C Insects reared at 25-27 degrees celsius and 14h:10h light:dark cycle in controlled environment 100ng DNA bisulfite converted using the EZ DNA Methylation-Lightning Kit DNA extracted using the DNeasy Blood & Tissue kit (QIAGEN) Bisulfite-converted DNA was amplified with a primer that contained part of an adaptor sequence plus four random nucleotides followed by two additional amplifications to add on the remaining adaptor sequence and to barcode the fragments. PCR products were purified using the DNA Clean & Concentrator-5â„¢ (Zymo Research).

characteristic: description

text: adult

characteristic: developmental stage

text: wild type

characteristic: genotype

text: 14 hour light, 10 hour dark cycle at 25 to 27 degrees celsius

characteristic: growth condition

text: Helicoverpa armigera

text: Helicoverpa armigera

text: Helicoverpa armigera

text: mixed pool of four male and four female

characteristic: sex

text: frozen in liquid nitrogen

characteristic: specimen with known storage state

text: Qiuxian

text: QX


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: TRUE

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: USA

characteristic: geo loc name

text: TRUE

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: human gut metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: TRUE

characteristic: public

text: human gut metagenome

text: American Gut Project

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: altitude

text: USA

characteristic: country

characteristic: depth

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

text: research facility

text: misc environment

text: sterile water

text: USA:CA:San Diego

text: TRUE

characteristic: latitude

characteristic: longitude

text: metagenome

text: UCSDMI

text: TRUE

text: TRUE

text: metagenome

text: American Gut Project


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: USA

text: TRUE

text: urban biome

characteristic: env biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: sterile water

text: USA:CA:San Diego

characteristic: geo loc name

text: TRUE

characteristic: host subject id

text: metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: TRUE

characteristic: public

text: metagenome

text: American Gut Project


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Using 12 novel PCR primers, the complete sequence of Saccharum hybrid cultivar China was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

text: Saccharum hybrid cultivar

text: Saccharum hybrid cultivar

text: leaf

characteristic: tissue type

text: Chloroplast isolation, sequencing and assembly of Saccharum hybrid cultivar China

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: cultivar

text: Using 12 novel PCR primers, the complete sequence of Saccharum hybrid cultivar SP70-1143 was sequenced with the Illumina platform. Reads were assembled with spades and polished with Pilon prior to annotation with a novel pipeline based on Verdant.

characteristic: description

text: Saccharum hybrid cultivar

text: Saccharum hybrid cultivar

text: leaf

text: Chloroplast isolation, sequencing and assembly of Saccharum hybrid cultivar SP70-1143


Lactococcus lactis

text: public

text: Lactococcus lactis

text: metagenome-assembled Lactococcus lactis genome from Maasdam Cheese with high percentage completeness.

text: Lactococcus lactis

text: Lactococcus lactis

text: Lactococcus lactis


text: Institute of Biotechnology, University of Helsinki

text: public

text: metagenome-assembled Propionibacterium freudenreichii subsp. shermanii genome from Maasdam Cheese with high percentage completeness.


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


E-MTAB-6344:BIT at 20% MIC_rep1

characteristic: ENA-CHECKLIST

text: Cardiff University

text: public

text: E-MTAB-6344:BIT at 20% MIC_rep1

tag: Namespace:Cardiff University

text: ArrayExpress

characteristic: broker name

text: Protocols: In total 7 test conditions (termed 1-7) and one control condition (growth in liquid media) were included, each with 4 biological replicates. The results from the test conditions were compared to the control, to determine differentially expressed genes when the industrial strain was exposed to the preservatives and product formulations. Test conditions included: 1) The preservative benzisothiazolinone (BIT) at 20% of the minimum inhibitory concentration (MIC) 2) BIT at 50% of the MIC 3) Phenoxyethanol (POE) at 20% of the MIC 4) POE at 50% of the MIC 5) BIT and POE in combination, both at 20% of the MIC 6) Unpreserved laundry detergent (LD) 7) Laundry detergent (LD) in combination with BIT at 20% of the MIC. After exposure to the control and test conditions for 24 hours the cultures for each of the sample replicates were promptly snap-cooled in an ethanol and dry ice bath, before centrifuging at 20,000 x g at 4ºC for 1 minute. The supernatant was removed and pellets were immediately snap-frozen and stored at -80ºC until required. Total RNA was extracted within one week of harvesting cells using the RiboPure™ RNA Purification Bacteria Kit (Ambion, Life Technologies Ltd, UK) according to the manufacturer’s instructions. A MICROBExpress™ bacterial mRNA enrichment kit (Ambion), was used to enrich for messenger RNA (mRNA) from the extracged total RNA. Complementary DNA (cDNA) library preparations and sequencing were carried out in collaboration with the Genomics Research Hub at Cardiff School of Biosciences. The Illumina® TruSeq® Stranded mRNA Sample Preparation Kit was used to prepare sequencing libraries with the low sample protocol. The manufacturer’s instructions describing the use of the protocol with previously isolated mRNA was followed; 5 μl of enriched mRNA within the Illumina recommended concentration range of 10 - 400 ng was added to 13 μl of the fragment, prime, finish mix to prepare sequencing libraries for each sample.

text: wild type

characteristic: genotype

text: benzisothiazolinone at 20% minimum inhibitory concentration

characteristic: growth condition

text: Pseudomonas aeruginosa

characteristic: organism

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: BIT at 20% MIC_rep1


E-MTAB-6344:BIT at 20% MIC_rep2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cardiff University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6344:BIT at 20% MIC_rep2

text: ArrayExpress

text: wild type

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: BIT at 20% MIC_rep2


E-MTAB-6344:BIT at 20% MIC_rep3

text: Cardiff University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6344:BIT at 20% MIC_rep3

text: ArrayExpress

text: wild type

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: BIT at 20% MIC_rep3

characteristic: title


E-MTAB-6344:BIT at 20% MIC_rep4

text: Cardiff University

text: public

text: E-MTAB-6344:BIT at 20% MIC_rep4

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: wild type

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: BIT at 20% MIC_rep4


E-MTAB-6344:BIT at 50% MIC_rep1

text: Cardiff University

text: public

text: E-MTAB-6344:BIT at 50% MIC_rep1

text: ArrayExpress

text: wild type

text: benzisothiazolinone at 50% minimum inhibitory concentration

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: BIT at 50% MIC_rep1