text: USDA-FS

text: public

text: AACT

text: Idaho Co, ID, USA

text: fisher

text: Idaho M. pennanti columbiana #2

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: Idaho/Montana

text: Martes pennanti

text: columbiana


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: TGCT

characteristic: barcode

text: Idaho Co, ID, USA

characteristic: collection site

text: fisher

characteristic: common name

text: Idaho M. pennanti columbiana #1

characteristic: longitude

text: Martes pennanti

characteristic: organism

text: Idaho/Montana

characteristic: region

text: Martes pennanti

text: columbiana

characteristic: subspecies

type: has member


text: USDA-FS

text: public

text: GATT

text: Humboldt Co, CA, USA

text: fisher

text: N. California M. pennanti pacifica #4

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: N. California

text: Martes pennanti

text: pacifica


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

text: public

text: TACT

text: Humboldt Co, CA, USA

text: fisher

text: N. California M. pennanti pacifica #3

text: Martes pennanti

text: N. California

text: Martes pennanti

text: pacifica


text: USDA-FS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GTAT

text: Humboldt Co, CA, USA

text: fisher

text: N. California M. pennanti pacifica #2

text: Martes pennanti

text: N. California

text: Martes pennanti

text: pacifica


text: USDA-FS

text: public

characteristic: Submitter Id

text: CCCT

characteristic: barcode

text: Idaho Co, ID, USA

characteristic: collection site

text: fisher

characteristic: common name

text: Idaho M. pennanti columbiana #5

text: Martes pennanti

text: Idaho/Montana

text: Martes pennanti

text: columbiana


text: USDA-FS

text: public

text: AGCT

text: Idaho Co, ID, USA

text: fisher

text: Idaho M. pennanti columbiana #4

characteristic: longitude

text: Martes pennanti

characteristic: organism

text: Idaho/Montana

characteristic: region

text: Martes pennanti

text: columbiana

characteristic: subspecies


text: USDA-FS

text: public

text: TGCT

text: Idaho Co, ID

text: fisher

text: Idaho M. pennanti columbiana #7

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: Idaho/Montana

text: Martes pennanti

text: columbiana


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

text: public

text: TTGT

text: Fresno Co, CA, USA

text: fisher

text: S. California M. pennanti pacifica #7

text: Martes pennanti

text: S. California

text: Martes pennanti

text: pacifica


text: USDA-FS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ACGT

text: Humboldt Co, CA, USA

text: fisher

text: N. California M. pennanti pacifica #5

text: Martes pennanti

text: N. California

text: Martes pennanti

text: pacifica


text: USDA-FS

text: public

characteristic: Submitter Id

text: ACGT

characteristic: barcode

text: Idaho Co, ID

characteristic: collection site

text: fisher

characteristic: common name

text: Idaho M. pennanti columbiana #8

text: Martes pennanti

text: Idaho/Montana

text: Martes pennanti

text: columbiana


text: USDA-FS

text: public

text: CTGT

text: Mineral Co, MT, USA

text: fisher

text: Montana M. pennanti columbiana #2

characteristic: longitude

text: Martes pennanti

characteristic: organism

text: Idaho/Montana

characteristic: region

text: Martes pennanti

text: columbiana

characteristic: subspecies


text: USDA-FS

text: public

text: GTAT

text: Lake of the Woods Co, MN, USA

text: fisher

text: Minnesota M. pennanti pennanti #1

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: Great Lakes--MN

text: Martes pennanti

text: pennanti


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

text: public

text: TACT

text: Lake of the Woods Co, MN, USA

text: fisher

text: Minnesota M. pennanti pennanti #2

text: Martes pennanti

text: Great Lakes--MN

text: Martes pennanti

text: pennanti


text: USDA-FS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GATT

text: Oneida Co, WI, USA

text: fisher

text: Wisconsin M. pennanti pennanti #1

text: Martes pennanti

text: Great Lakes--WI

text: Martes pennanti

text: pennanti


text: USDA-FS

text: public

characteristic: Submitter Id

text: TCAT

characteristic: barcode

text: Oneida Co, WI, USA

characteristic: collection site

text: fisher

characteristic: common name

text: Wisconsin M. pennanti pennanti #2

text: Martes pennanti

text: Great Lakes--WI

text: Martes pennanti

text: pennanti


text: USDA-FS

text: public

text: TGCT

text: Fresno Co, CA, USA

text: fisher

text: S. California M. pennanti pacifica #4

characteristic: longitude

text: Martes pennanti

characteristic: organism

text: S. California

characteristic: region

text: Martes pennanti

text: pacifica

characteristic: subspecies


text: USDA-FS

text: public

text: GATT

text: Near Williams Lake, BC, CAN

text: fisher

text: British Columbia M. pennanti columbiana #8

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: British Columbia

text: Martes pennanti

text: columbiana


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

text: public

text: CTGT

text: Near Williams Lake, BC, CAN

text: fisher

text: British Columbia M. pennanti columbiana #9

text: Martes pennanti

text: British Columbia

text: Martes pennanti

text: columbiana


text: USDA-FS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: AACT

text: Near Williams Lake, BC, CAN

text: fisher

text: British Columbia M. pennanti columbiana #10

text: Martes pennanti

text: British Columbia

text: Martes pennanti

text: columbiana


text: USDA-FS

text: public

characteristic: Submitter Id

text: ACGT

characteristic: barcode

text: Fresno Co, CA, USA

characteristic: collection site

text: fisher

characteristic: common name

text: S. California M. pennanti pacifica #1

text: Martes pennanti

text: S. California

text: Martes pennanti

text: pacifica


text: USDA-FS

text: public

text: AACT

text: Fresno Co, CA, USA

text: fisher

text: S. California M. pennanti pacifica #5

characteristic: longitude

text: Martes pennanti

characteristic: organism

text: S. California

characteristic: region

text: Martes pennanti

text: pacifica

characteristic: subspecies


text: USDA-FS

text: public

text: CCCT

text: Fresno Co, CA, USA

text: fisher

text: S. California M. pennanti pacifica #3

characteristic: description

characteristic: genbank

characteristic: lane

characteristic: latitude

text: Martes pennanti

text: S. California

text: Martes pennanti

text: pacifica


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-FS

characteristic: INSDC center name

text: public

text: CTGT

text: Humboldt Co, CA, USA

text: fisher

text: N. California M. pennanti pacifica #1

text: Martes pennanti

text: N. California

text: Martes pennanti

text: pacifica


text: USDA-FS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: TTGT

text: Near Williams Lake, BC, CAN

text: fisher

text: British Columbia M. pennanti columbiana #11

text: Martes pennanti

text: British Columbia

text: Martes pennanti

text: columbiana


text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: PDP5047754-sc-2011-04-19T11:13:35Z-1065454

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047755-sc-2011-04-19T11:13:37Z-1065456

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047746-sc-2011-04-19T11:03:43Z-1065437

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047747-sc-2011-04-19T11:03:43Z-1065439

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047748-sc-2011-04-19T11:08:31Z-1065442

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047749-sc-2011-04-19T11:08:32Z-1065443

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047750-sc-2011-04-19T11:08:33Z-1065445

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047751-sc-2011-04-19T11:08:34Z-1065447

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047753-sc-2011-04-19T11:08:35Z-1065452

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047733-sc-2011-04-19T10:43:41Z-1065413

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047734-sc-2011-04-19T10:48:37Z-1065415

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047684-sc-2011-04-13T18:08:57Z-1065507

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047683-sc-2011-04-13T18:08:56Z-1065505

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047761-sc-2011-04-19T11:18:36Z-1065468

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047760-sc-2011-04-19T11:18:36Z-1065466

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047762-sc-2011-04-19T11:18:37Z-1065470

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047757-sc-2011-04-19T11:13:38Z-1065459

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047756-sc-2011-04-19T11:13:37Z-1065457

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047759-sc-2011-04-19T11:18:35Z-1065464

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047758-sc-2011-04-19T11:13:38Z-1065462

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047743-sc-2011-04-19T11:03:36Z-1065432

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047742-sc-2011-04-19T11:03:35Z-1065429

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047741-sc-2011-04-19T10:58:46Z-1065427

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047740-sc-2011-04-19T10:58:46Z-1065425

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047738-sc-2011-04-19T10:58:45Z-1065422

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047737-sc-2011-04-19T10:53:31Z-1065421

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047736-sc-2011-04-19T10:48:39Z-1065418

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047735-sc-2011-04-19T10:48:38Z-1065416

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047745-sc-2011-04-19T11:03:42Z-1065435

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PDP5047744-sc-2011-04-19T11:03:41Z-1065433

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: Sample F from Figure 2D

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: Sample E from Figure 2D

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: Sample H from Figure 2D

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: WT untreated

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: WT IFNb 6 hours

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: Sample C from Figure 2D

text: Mus musculus

text: Mus musculus


text: HU-MCB

text: public

text: house mouse

text: Sample D from Figure 2D

text: Mus musculus

text: Mus musculus


text: TRON

text: public

text: Ambion

characteristic: biosourceprovider

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: We purchased total RNA from Ambion (Austin, USA). Each tissue samples was pooled from multiple donors. Librarys were prepared as in Armour CD, Castle JC, Chen R, Babak T, Loerch P, et al. (2009) Digital transcriptome profiling using selective hexamer priming for cDNA synthesis. Nat Methods 6: 647-649.

text: Homo sapiens

text: Homo sapiens

text: hypothalamus

characteristic: organism part

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: colon

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: skeletal muscle

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: heart

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: ovary

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: testes

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens


text: TRON

text: public

text: Ambion

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: adipose

text: Homo sapiens


text: public

text: ArrayExpress

text: fruit fly

text: Protocols: Chromatin was obtained from male 3rd instar larvae salivary glands as previously described (Legube et al, 2006). In brief, collected salivary glands (10 per ChIP) were fixed in 1 mL of fixing solution (50 mM HEPES at pH 7.6, 100 mM NaCl, 0.1 mM EDTA at pH 8, 0.5 mM EGTA at pH 8, 2% formaldehyde) for 15 min at room temperature on a rotating wheel. The glands were centrifuged at 2000 rpm for 1 min, washed once in PBS with 0.01% Triton X-100 and 0.125 M glycine; then washed for 10 mins each in 1 ml of buffer A (0.25% Triton X-100, 10 mM EDTA (pH 8), 0.5 mM EGTA (pH 8), 10 mM Tris (pH 8)) and buffer B (200 mM NaCl, 10 mM Tris (pH 8), 10 mM EDTA (pH 8), and 0.5 mM EGTA (pH 8)). The glands where then resuspended in 500 _L of sonication buffer (10 mM Tris at pH 8, 1 mM EDTA at pH 8, 0.5 mM EGTA at pH 8.0) and sonicated 8 min (pulsed eight times for 30 sec, paused for 30 sec, high) in a Bioruptor (Cosmo Bio). Immunoprecipitation was then performed on 500 _L of chromatin from salivary glands, according to Orlando et al. (1997), using 3 _L of a polyclonal antibody against NSL-1, MCRS2.

text: 3rd instar larvae

characteristic: developmental stage

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: pearl danio

text: Danio albolineatus

text: Danio albolineatus


text: SC

text: public

text: pig

text: Sus scrofa

text: Sus scrofa


characteristic: ENA-FIRST-PUBLIC

text: CRUK-CRI

text: public

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: RPMI 1640 medium por DMEM supplemented with 10% inactivated FBS, l-glutamine and PEST at 37C with 5% CO2. Antibodies were from Santa Cruz Biotechnology (sc-543), abcam (ab5089 and ab23738) and Millipore (07-729) and ChIP was performed using 10e7-10e8 cells per reaction. Briefly, crosslinking of protein and DNA was done in room temperature for 10 minutes with serum free media containing 0,37 % formaldehyde. Cells were scraped and washed in PBS before treatment of cell lysis buffer with protease inhibitors on ice. Pelleted nuclei were resuspeded in RIPA buffer and a BioRuptor (Diagenode) was used to sonicate DNA. ChIP was performed with 10 micrograms of antibody. ChIP DNA was gel fractionated and amplified to create two size libraries corresponding to insert sizes of 200-300 bp. For DNase I hypersensitivity was performed as previously described (Eeckhoute et al., 2006). Digested DNA was run on a gel and the digested material (between 200bp and 500bp in size). For all ChIPs washing was done six times with RIPA buffer and once with TE-buffer and DNA-protein complexes were eluted from beads twice with 200 microlitres of 0.1 M NaHCO3 and 1 % SDS and treated with RNAseA at 65C for 6 hours and Proteinase K at 45C over night. Chromatin was amplified with Solexa protocol chromatin amplification.

text: breast cancer

characteristic: disease state

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CRUK-CRI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-223:full_ER_ChIP_ZR751_exp1_lane1

text: ArrayExpress

text: human

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


text: CRUK-CRI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-223:full_ER_ChIP_ZR751_exp2_lane2

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


text: CRUK-CRI

text: public

text: E-MTAB-223:E2_ER_ChIP_siFoxA1_exp1_lane2

text: ArrayExpress

characteristic: broker name

text: human

text: breast cancer

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female


text: CRUK-CRI

text: public

text: E-MTAB-223:E2_ER_ChIP_siFoxA1_exp1_lane1

text: ArrayExpress

text: human

characteristic: description

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

text: CRUK-CRI

text: public

text: E-MTAB-223:full_ER_ChIP_ZR751_exp2_lane1

text: ArrayExpress

text: human

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


text: CRUK-CRI

text: public

text: E-MTAB-223:full_ER_ChIP__MCF7_exp1_lane2

text: ArrayExpress

characteristic: cell line

text: human

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


text: CRUK-CRI

text: public

text: E-MTAB-223:full_ER_ChIP_ZR751_exp1_lane2

text: ArrayExpress

text: human

text: breast cancer

characteristic: disease state

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female


text: Individual: NA12814

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

text: Protocols: This method involves several steps that are designed to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing. The first step involves purifying the poly-A containing mRNA molecules using poly-T oligo-attached magnetic beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then, the cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and a high concentration of random hexamer primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. Finally, the short cDNA fragments are prepared for sequencing on the Illumina Genome Analyzer.

characteristic: individual

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12003

text: Population: HapMap CEU

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA06986

text: Population: HapMap CEU

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA12414

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: description

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12892

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: lymphoblastoid

characteristic: cell type

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12005

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: individual

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA11931

text: Population: HapMap CEU

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11994

text: Population: HapMap CEU

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12828

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA12489

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: common name

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12717

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12813

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

characteristic: individual

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA11840

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


text: Individual: NA07000

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12874

text: Population: HapMap CEU

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA07357

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11920

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12761

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

characteristic: individual

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA06985

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12750

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


text: Individual: NA11829

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA12154

text: Population: HapMap CEU

text: SC

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11831

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12234

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

characteristic: description

characteristic: individual

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12004

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12249

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11918

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA12155

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA10847

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA07051

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA07037

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: individual

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12044

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11894

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA11830

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


text: Individual: NA12156

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

characteristic: Submitter Id

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12045

text: Population: HapMap CEU

text: SC

text: public

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

characteristic: cell type

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12762

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12763

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: individual

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11992

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA06994

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA10851

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


text: Individual: NA12716

text: Population: HapMap CEU

text: SC

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

characteristic: cell type

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12872

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12751

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11995

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: individual

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA11993

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12891

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11881

text: Population: HapMap CEU

text: SC

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12760

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

characteristic: cell type

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12144

text: Population: HapMap CEU

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA07347

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: description

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: Individual: NA12815

text: Population: HapMap CEU

text: SC

text: public

characteristic: SRA accession

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: individual

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA11832

characteristic: Experimental Factor: INDIVIDUAL

text: Population: HapMap CEU

characteristic: Experimental Factor: POPULATION

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


text: Individual: NA12043

text: Population: HapMap CEU

text: SC

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: lymphoblastoid

text: human

text: caucasian

characteristic: observation

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Individual: NA12873

text: Population: HapMap CEU

characteristic: External Id

text: SC

text: public

text: ArrayExpress

text: lymphoblastoid

text: human

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

characteristic: population

text: Homo sapiens


text: Individual: NA19238

text: Population: HapMap YRI

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: lymphoblastoid

characteristic: cell type

text: human

text: yoruba

text: Homo sapiens

text: Homo sapiens

text: HapMap YRI

text: Homo sapiens


text: Individual: NA12776

text: Population: HapMap CEU

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: lymphoblastoid

text: human

characteristic: common name

text: caucasian

text: Homo sapiens

text: Homo sapiens

text: HapMap CEU

text: Homo sapiens


name: cultivated rice indica Guangluai 4 transcriptome

characteristic: ENA-FIRST-PUBLIC

text: NCGR

text: public

characteristic: SRA accession

text: long-grained rice

text: Guangluai 4

characteristic: cultivar

text: Rice transcriptome profiling at single-nucleotide resolution by RNA-seq

characteristic: description

text: Oryza sativa Indica Group

text: Oryza sativa Indica Group


name: cultivated rice indica 93-11 transcriptome

text: NCGR

text: public

text: long-grained rice

text: Oryza sativa Indica Group

characteristic: organism

text: Oryza sativa Indica Group


text: USDA-FS

text: public

characteristic: Submitter Id

text: TTGT

characteristic: barcode

text: yellow rail

text: Yellow rail

characteristic: lane

text: Coturnicops noveboracensis

text: Coturnicops noveboracensis


characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Sequencing of PFCLIN genes MSP2 and PF10-0355 genes at ultra high coverage

characteristic: sample description

text: Plasmodium falciparum

text: PFCLIN

characteristic: strain


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: SC

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Sequencing of 3D7 genes MSP2 and PF10-0355 genes at ultra high coverage

text: Plasmodium falciparum


text: SC

characteristic: INSDC last update

text: public

text: malaria parasite P. ovale

text: Plasmodium ovale

text: Plasmodium ovale


characteristic: ATCC(R) Number

text: INCTC

text: public

characteristic: INSDC status

text: human

text: HCC1954 cell lines obtained from ATCC(R) and were maintained in RPMI medium containing 10% fetal bovine serum (FBS) and non-essential amino acids.

text: Homo sapiens

text: Homo sapiens

text: Human cell line HCC1954

characteristic: title


text: SC

text: public

characteristic: SRA accession

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: CEPH-HGDP sample ID

text: MPI-EVA

text: public

characteristic: Submitter Id

text: human

text: Human CEPH-HGDP individual HGDP00778

characteristic: description

text: China

characteristic: geographic location (country)

text: Homo sapiens

text: Han

characteristic: population

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00927

text: Nigeria

text: Homo sapiens

text: Yoruba

text: Homo sapiens


characteristic: External Id

text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00521

text: France

text: Homo sapiens

text: French

text: Homo sapiens


text: MPI-EVA

characteristic: INSDC center name

text: public

text: human

text: Human CEPH-HGDP individual HGDP01224 (Mongolian)

text: Mongolia

text: Homo sapiens

text: Mongolian

text: Homo sapiens


text: MPI-EVA

characteristic: INSDC first public

text: public

text: human

text: Human CEPH-HGDP individual HGDP01029

text: Namibia

text: Homo sapiens

text: San

text: Homo sapiens


text: MPI-EVA

characteristic: INSDC last update

text: public

text: human

text: Human CEPH-HGDP individual HGDP00542

text: New Guinea

text: Homo sapiens

text: Papuan

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00665 (Sardinian)

text: Sardinia

text: Homo sapiens

text: Sardinian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00456 (Mbuti Pygmy)

text: Congo

text: Homo sapiens

text: Mbuti Pygmy

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00711 (Cambodian)

text: Cambodia

text: Homo sapiens

text: Cambodian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00998 (Karitiana Native American)

text: Brazil

text: Homo sapiens

text: Karitiana Native American

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00551 (Papuan)

text: New Guinea

text: Homo sapiens

text: Papuan

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00491 (Bougainville Melanesian)

text: Bougainville

text: Homo sapiens

text: Bougainville Melanesian

text: Homo sapiens


E-MTAB-460:Zebrafish adult brain

text: SC

text: public

text: Singapore

characteristic: StrainOrLine

text: E-MTAB-460:Zebrafish adult brain

text: ArrayExpress

characteristic: broker name

text: zebrafish

text: Protocols: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at -70 C Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions. Pellets were re-suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND-1000 Spectrophotometer (Axon Instruments).

text: adult

characteristic: developmental stage

text: Danio rerio

text: Danio rerio

text: brain

characteristic: organism part

text: Danio rerio

text: mixed

characteristic: sex

text: Zebrafish adult brain


E-MTAB-460:Zebrafish adult heart

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Singapore

text: E-MTAB-460:Zebrafish adult heart

text: ArrayExpress

text: zebrafish

characteristic: common name

text: adult

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: mixed

text: Zebrafish adult heart

characteristic: title


E-MTAB-460:Zebrafish adult head kidney

text: SC

text: public

text: Singapore

text: E-MTAB-460:Zebrafish adult head kidney

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

characteristic: description

text: adult

text: Danio rerio

characteristic: organism

text: Danio rerio

text: head kidney

text: Danio rerio

text: mixed

text: Zebrafish adult head kidney


E-MTAB-460:Zebrafish adult swim bladder

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: Singapore

text: E-MTAB-460:Zebrafish adult swim bladder

text: ArrayExpress

text: zebrafish

text: adult

text: Danio rerio

text: Danio rerio

text: swim bladder

text: Danio rerio

text: mixed

text: Zebrafish adult swim bladder


Pug

unit: ug/L

characteristic: HA concentration

text: SLU

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Pug

text: dog

text: Pug dog breed

text: Canis lupus familiaris

text: Canis lupus familiaris


text: SLU

characteristic: INSDC last update

text: public

text: dog

text: Chinese Sharpei dog meatmouth type

text: Canis lupus familiaris

text: Canis lupus familiaris


text: SLU

text: public

text: dog

text: Chinese Sharpei dog bonemouth type

text: Canis lupus familiaris

text: Canis lupus familiaris


Neapolitan Mastiff

text: SLU

text: public

text: Neapolitan Mastiff

text: dog

text: Neapolitan Mastiff dog breed

text: Canis lupus familiaris

text: Canis lupus familiaris


Poodle

text: SLU

text: public

text: Poodle

text: dog

text: TODO:Poodle dog breed

text: Canis lupus familiaris

text: Canis lupus familiaris


text: public

text: GABIPD

characteristic: broker name

text: rye

text: winter rye (Secale cereale L.) inbred line Lo152 of KWS-LOCHOW GMBH

characteristic: inbred line

text: Secale cereale

text: Secale cereale

text: winter rye inbred line Lo152


text: public

text: GABIPD

text: rye

text: winter rye (Secale cereale L.) inbred line Lo225 of KWS-LOCHOW GMBH

text: Secale cereale

text: Secale cereale

text: winter rye inbred line Lo225


text: public

text: GABIPD

text: rye

text: Russian winter rye (Secale cereale L.) inbred line P87 developed at the Institue of Genetics and Cytology, Minsk, Belarus, by Dr. T. S. Schilko, see further Korzun et al. (1998) Theor Appl Genet 96: 203-208

text: Secale cereale

text: Secale cereale

text: winter rye inbred line P87


text: public

text: GABIPD

text: rye

text: Russian winter rye (Secale cereale L.) inbred line P105 developed at the Institue of Genetics and Cytology, Minsk, Belarus, by Dr. T. S. Schilko, see further Korzun et al. (1998) Theor Appl Genet 96: 203-208

text: Secale cereale

text: Secale cereale

text: winter rye inbred line P105


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: STOWERS INSTITUTE FO

text: public

tag: Namespace:STOWERS INSTITUTE FO

text: 2 to 4 hours

characteristic: age

characteristic: bio source type

text: Zeitlinger Lab

characteristic: biosourceprovider

text: ArrayExpress

text: fruit fly

text: Protocols: Flies of all six species were raised at 25C and 60% humidity on corn syrup based food. Two to four days post enclosure flies were moved to a plexiglas cage and fed yeast paste on 150mm apple agar plates. All collections resulted in embryos 2-4 hours in age (For D. pseudoobscura, opuntia powder was also sprinkled on apple agar plates to increase numbers of eggs laid). Plates were left in cages for 2 hours allowing flies to lay eggs on them. Afterwards plates were removed and allowed to incubate for an additional 1.5h at 25C till harvest started, at which point bleach was added to the plate for two minutes to dechorionate embryos. Collection windows for most species were 2-4 hours post egg laying to match D. melanogaster embryonic development. We observed a delay in D. pseudoobscura, and acceleration in D. simulans embryonic development, which are consistent with an earlier study about heterochrony in Drosophila embryonic development (PMID:10618397). Therefore, for these two species, collection windows were adjusted empirically based on two criteria: a. to ensure majority of embryos were within Bownes stage 5-8; b. to obtain optimal signal/noise ratio from ChIP-seq experiments. Collection plates were removed after 2 hours of egg laying and were incubated for additional 0.5, 1.5, or 2.5 hours at 25C until the start of harvest for 1-3, 2-4, or 3-5 hour collections, respectively. Embryos were fixed using a modified protocol from Furlong et.al. Briefly, embryos were transferred to a tube containing PBT (PBS with 0.1% Triton) and allowed to settle. After PBT being removed, embryos were crosslinked by being vortexed in a mixture of fixing buffer (50 mM HEPES, pH 7.5, 1 mM EDTA, 0.5 mM, EGTA, 100 mM NaCl, 1.8% formaldehyde) and heptane for 15 minutes at room temperature. To stop crosslinking, fixing solution and heptane were removed, and 2.5 M glycine in PBT was added to fixed embryos before they were vortexed for 1 minute. Embryos were washed with PBT and either used for preparation of whole cell extract for chromatin immunoprecipitation or rapidly frozen in liquid nitrogen and stored at -80C. For staging embryos, some fixed embryos were vortexed in methanol:heptane (V:V=1:1) for 10 minutes for devitellinization, and washed twice by methanol before being stored in methanol at -20C. ChIPs were done using a modified protocol from Furlong lab (http://furlonglab.embl.de/methods/extras/ChIP-on-chip-Drosophila.pdf). On the day before ChIP, 100 ul protein A-conjugated Dynobeads (Invitrogen 100-02D) were washed three times by block solution (PBS with 5 mg/ml BSA), and incubated with 20 ul affinity purified rabbit polycolonal anti-Drosophila melanogaster Twist antibody (a generous gift from Levine lab and Furlong lab) at 4C overnight. To prepare nuclear extract, embryos cross-linked in 1.8% formaldehyde were resuspended in buffer A1 (15 mM HEPES, pH 7.5, 15 mM NaCl, 60 mM KCl, 4 mM MgCl2, 0.5 % Triton X-100, 0.5 mM DDT, freshly added complete EDTA-free protease inhibitor cocktail (Roche 11873580001)), and homogenized six times by both pestles on ice. Nuclei were precipitated by centrifuge, washed three times by buffer A1, and resuspended in buffer A2 (15mM HEPES, pH 7.5, 140 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 % Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 0.5% N-lauroylsarcosine, freshly added complete EDTA-free protease inhibitor cocktail (Roche 11873580001)). Chromatin was fragmented by sonication to the average size of 400-500 bp, and the soluble fraction (whole cell extract, WCE) was precipitated by centrifuge. For each ChIP, 300 ul WCE (100-120 mg embryos) was incubated with pre-treated protein A-conjugated Dynobeads overnight at 4C by rotating. Beads were washed four to five times by RIPA buffer (50 mM HEPES, pH 7.5, 1 mM EDTA, 0.7% Na deoxycholate, 1% IGEPAL CA-630, 0.5 M LiCl), and bound chromatin was eluted by 100 ul elution buffer (50 mM Tris + 1% SDS) on a thermomixer at 65C for 20 minutes with agitation. Supernatant was transferred and 100 ul TE was added to each ChIP sample before reverse crosslinking. For control, 50 ul WCE was mixed with 50 ul TE and 100 ul elution buffer. Reverse crosslinking was done by incubating samples at 65C for six hours. Reverse crosslinked DNA was then first treated with RNase A (0.2 ug/ul) and then with proteinase K (0.2 ug/ul) for two hours at 37C and 55C, respectively. DNA was cleaned with phenol (Sigma P4557) and phenol-chloroform-isoamyl alcohol mixture (Sigma 77617). For precipitation, glycogen and NaCl were added to each sample to the final concentration of 0.15 ug/ul and 200 mM, respectively. DNA was precipitated by 2.5 fold (v:v) 100% ethanol at -80C for at least two hours before DNA being precipitated by centrifuge and washed with 70% ethanol at 4C. Precipitated DNA was solved in TE. Level of Twist enrichment was monitored by real-time PCR. For D. melanogaster, regions within the enhancers of brinker and rhomboid genes were chosen as positive controls, while a region in chromosome 2L as negative control (NonG). Similarly, for individual non-melanogaster species, a region upstream of tup gene, and regions within the open reading frame of the othologues of D. melanogaster Dscam gene were chosen as positive control, while orthologous regions of NonG was used as negative control. Sequences for oligos used in PCR are available upon request.

text: embryonic stage 5 to 8

characteristic: developmental stage

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

text: PLANT AND FOOD RESEARCH

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-587:Inflorescences-Low_amount_material-SEP3_IP-rep1

tag: Namespace:PLANT AND FOOD RESEARCH

characteristic: Submitter Id

text: 5-7 weeks

text: ArrayExpress

text: thale cress

characteristic: common name

text: Protocols: Arabidopsis thaliana wild-type (Col-0), plants were grown under standard greenhouse conditions (20C, long day light regime: 16 h light, 8 hours dark cycle). Flower material was harvested from primary and secondary inflorescences of 5-7 weeks old plants Inflorescences were harvested and fixed using FAA following a protocol published in Gomez-Mena et al. 2005 (PMID: 15634696) with modifications in de Folter et al. 2007 (PMID: 17868439)

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Inflorescence

characteristic: organism part

text: Arabidopsis thaliana

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: PLANT AND FOOD RESEARCH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-587:Inflorescences-Low_amount_material-SEP3_IP-rep2

text: 5-7 weeks

text: ArrayExpress

text: thale cress

text: Protocols: Arabidopsis thaliana, wild-type (Col-0), plants were grown under standard greenhouse conditions (20C, long day light regime: 16 h light, 8 hours dark cycle). Flower material was harvested from primary and secondary inflorescences of 5-7 weeks old plants Inflorescences were harvested and fixed using FAA following a protocol published in Gomez-Mena et al. 2005 (PMID: 15634696) with modifications in de Folter et al. 2007 (PMID: 17868439)

characteristic: description

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: Inflorescence

text: Arabidopsis thaliana


text: PLANT AND FOOD RESEARCH

characteristic: INSDC last update

text: public

text: E-MTAB-587:Inflorescences-Low_amount_material-OverAmplified-SEP3_IP-rep1

text: 5-7 weeks

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Inflorescence

text: Arabidopsis thaliana


text: SC

text: public

text: zebrafish

text: Danio rerio

text: Danio rerio

text: Tuebingen

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: CRUK-CRI

text: public

text: ArrayExpress

text: Norway rat

text: Protocols: Samples were prepared by direct perfusion of the tissue with buffered salt solution, followed by % formaldehyde. After 10 minutes, the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization, the cells were rinsed with PBS. The animal tissues were crosslinked with formaldehyde treatment and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA - version 2.2) with the following modifications. The ChIP-enriched DNA was not further fragmented. After end-repair and addition of an 'A' base to the 3' ends, the adapters were ligated to the ends of the DNA Fragments using 2 ul of fortyfold diluted 'Adapter oligo mix' in a total reaction volume of 25 ul. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5 ul of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2% agarose gel and a gel slice containing the 200-300 bp fragments was excised. The flowcells were prepared and processed according to the manufacturer's protocols, with single-end sequencing for 36 to 45 cycles.

text: adult

characteristic: developmental stage

text: Rattus norvegicus

text: Rattus norvegicus

text: liver

text: Rattus norvegicus

text: male

characteristic: sex


text: CRUK-CRI

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: StrainOrLine

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Step1 RNA extraction 1. Excise 25 mg liver tissue sample (on dry ice) into Precellys tubes CK28-R, bertin technology 2. Remove the sample tube from the dry ice and immediately pipet 700 ul QIAzol Lysis Reagent into each sample tube. 3. Homogenize immediately on the TissueLyser for 2x10 5000 with 10 break 4. Place the sample tube containing the homogenate on the benchtop at room temperature (15-25C) for 5 min. This step promotes dissociation of nucleoprotein complexes. 5. Add 140 ul chloroform and shake it vigorously for 15s. Thorough mixing is important for subsequent phase separation. 6. Place the sample tube on the benchtop at room temperature for 2-3 min. 7. Centrifuge at >6000 x g (max. 9000 x g for Precellys tubes) for 15 min at 4C. After centrifugation, the sample separates into 3 phases: an upper, colorless, aqueous phase containing RNA; a white interphase enriched with DNA; and a lower, red, organic phase containing proteins. For tissues with an especially high fat content, an additional, clear phase may be visible below the red, organic phase. The volume of the aqueous phase should be approximately 350 ul. 8. Transfer the upper aqueous phase to a new reaction tube. Centrifuge again if a little bit of the red organic phase was transferred accidentally. This is important because it will interfere with the downstream precipitation. 9. Add 1 Vol% of isopropanol and mix thoroughly. Incubate 15 min at RT 10. Centrifuge full-speed, 20 min, 4C. 11. Wash with 750 ul 75% EtOH 12. Centrifuge full-speed, 10 min, 4C 13. Allow pellet to air-dry 14. Dissolve pellet in 20-50 ul RNase-free water Step2 , Turbo Dnase treat (Ambion AM1907) 10ug Total RNA, 1/10th volume DNase buffer (10ul), Turbo DNase enzyme (2ul), Water (to 100ul) Incubate 37C 30 minutes (mix inactivation agent) 0.15 volume inactivation agent (15ul) Step4, Ribominus treat (Invitrogen K1550-02) Hybridization Step Instructions are provided below to perform hybridization for 1-10ug of your total RNA sample with the RiboMinus(tm) Eukaryote Probe. To process >10 ug total RNA sample, divide your sample into two samples, each containing <10 ug total RNA. 1. Set a water bath or heat block to 70-75C. 2. To a sterile, RNase-free 1.5 mL microcentrifuge tube, add the following: Total RNA (1-10 ug): <10 uL, RiboMinus(tm) Probe (15 pmol/uL): 10uL, Hybridization Buffer: 100 uL. 3. Incubate the tube at 70-75C for 5 minutes to denature RNA. 4. Allow the sample to cool to 37C slowly over a period of 30 minutes by placing the tube in a 37C water bath. To promote sequence-specific hybridization, it is important to allow slow cooling. Do not cool samples quickly by placing tubes in cold water. 5. While the sample is cooling down, proceed to Preparing Beads. 1. Resuspend RiboMinus(tm) Magnetic Beads in its bottle by thorough vortexing. 2. Pipet 750 uL of the bead suspension into a sterile, RNase-free, 1.5 mL microcentrifuge tube. 3. Place the tube with the bead suspension on a magnetic separator for 1 minute. The beads settle to the tube side that faces the magnet. Gently aspirate and discard the supernatant. 4. Add 750 uL sterile, DEPC Water to the beads and resuspend beads by slow vortexing. 5. Place tube on a magnetic separator for 1 minute. Aspirate and discard the supernatant. 6. Repeat Steps 4-5 once. 7. Resuspend beads in 750 uL Hybridization Buffer and transfer 250 uL beads to a new tube and maintain the tube at 37C for use at a later step. 8. Place the tube with 500 uL beads on a magnetic separator for 1 minute. Aspirate and discard the supernatant. 9. Resuspend beads in 200 uL Hybridization Buffer and keep the beads at 37C until use. Removing rRNA 1. After the incubation at 37C for 30 minutes of the hybridized sample (above), briefly centrifuge the tube to collect the sample to the bottom of the tube. 2. Transfer the sample (~120 uL) to the prepared RiboMinus(tm) Magnetic beads from Step 9 (Preparing Beads, above). Mix well by pipetting up and down or low speed vortexing. 3. Incubate the tube at 37C for 15 minutes. During incubation, gently mix the contents occasionally. Briefly centrifuge the tube to collect the sample to the bottom of the tube. 4. Place the tube on a magnetic separator for 1 minute to pellet the rRNA-probe complex. Do not discard the supernatant. The supernatant contains RiboMinus(tm) RNA. 5. Place the tube with 250 uL beads from Step 7 (Preparing Beads, above) on a magnetic separator for 1 minute. Aspirate and discard the supernatant. 6. To this tube of beads, add ~320 uL supernatant containing RiboMinus(tm) RNA from Step 4, above. Mix well by pipetting up and down or low speed vortexing. 7. Incubate the tube at 37C for 15 minutes. During incubation, gently mix the contents occasionally. Briefly centrifuge the tube to collect the sample to the bottom of the tube. 8. Place the tube on a magnetic separator for 1 minute to pellet the rRNA-probe complex. Do not discard the supernatant as the supernatant contains RiboMinus(tm) RNA. Concentrating RiboMinus(tm) RNA Using Ethanol Precipitation To ensure recovery of smaller (<200 nt) RNA, concentrate the RiboMinus(tm) RNA using ethanol precipitation as described below or using a modified RiboMinus(tm) Concentration Module (Cat. no. K1550-05) protocol with 60% ethanol (protocol included with the kit). 1. Transfer the RiboMinus(tm) RNA sample into a clean, RNAse-free 1.5 mL or 2 mL microcentrifuge tube. 2. Add the following components to RiboMinus(tm) RNA: - 1 mL glycogen (20 mg/mL) - 1/10th sample (eluted RNA) volume (30 uL for this protocol) of 3 M sodium acetate - 2.5X sample volumes (750 uL for this protocol) of 100% ethanol Step5, Fractionate RNA (Ambion AM8740) Catalog #: AM8740 Ambion For fragmentation of 2-20 ug of RNA, the recommended reaction volume is 10 uL. 1. Bring the RNA sample volume to 9 uL with Nuclease-free Water (Ambion Cat #AM9938). 2. Add 1 uL of the 10X Fragmentation Buffer to the RNA sample. 3. Mix, spin briefly,and incubate at 70C for 15 min in a heating block, thermocycler, or water bath. 4. Add 1 uL of the Stop Solution, place it on ice until use, or store it at -80C. Step6, PolyA tail (Ambion AM1350) Amount Component 20 uL RNA 36 uL Nuclease-free Water 20 uL 5X E-PAP Buffer 10 uL 25 mM MnCl2 10 uL 10 mM ATP 4 uL E-PAP 37C 1 Hour Step7, Phenol clean up Sigma P1944 Add 200ul Nuclease free water to reaction Add 200ul Acidic Phenol Chloroform, mix Spin 14,000g 15 minutes Take top aqueous layer add to 3x volumes 100% Ethanol Spin 4C 14,000g 15 minutes Wash pellet 70% Ethanol ( Nuclease free water ) 4C 14,000g 5 minutes Air dry 5 minutes Dissolve 3.5ul Water Step8, Smarter PCR cDNA synthesis kit Cat. Nos. 634925 1-3.5 ul RNA (1 ng-1 ug of poly A+ RNA or 2 ng-1 ug total RNA)* 1 ul 3' SMART CDS Primer II A (12 uM) x ul Deionized H2O 4.5 ul Total Volume Incubate the tubes at 72C in a hot-lid thermal cycler for 3 min, then reduce the temperature to 42C for 2 min. Prepare a Master Mix for all reaction tubes at room temperature by combining the following reagents in the order shown: 2 ul 5X First-Strand Buffer 0.25 ul DTT (100 mM) 1 ul dNTP Mix (10 mM ) 1 ul SMARTer II A Oligonucleotide (12 uM) 0.25 ul RNase Inhibitor 1 ul SMARTScribe(tm) Reverse Transcriptase (100 U)* 5.5 ul Total Volume added per reaction Incubate the tubes at 42C for 1 hour. Terminate the reaction by heating the tubes at 70C for 10 min. Add 40 ul of TE buffer if you used total RNA as the starting material. Prepare a PCR Master Mix for all reactions, plus one additional reaction. Combine the following reagents in the order shown: 74 ul Deionized H2O 10 ul 10X Advantage 2 PCR Buffer 2 ul 50X dNTP Mix (10 mM) 2 ul 5' PCR Primer II A (12 uM) 2 ul 50X Advantage 2 Polymerase Mix 90 ul Total Volume per reaction Place reaction in Thermal cycler 95C 2 minutes, 94C 15sec, 65C 30sec, 68C 30sec, 20 cycles, 68C 5 minutes, DNA purification column elute in 30ul Step8 RSA I digest NEB Cat no. R0167R0167 30ul PCR 4ul NEB Buffer 4 5ul Water 1ul Enzyme Incubate 37C 4 hours Stop reaction 68C 20 minutes. Run product on 2% agarose gel should see a smeared product >200bp.

text: adult

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male


characteristic: ENA-LAST-UPDATE

text: CRUK-CRI

text: public

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: Step1, RNA extraction 1. Excise 25 mg liver tissue sample (on dry ice) into Precellys tubes CK28-R, bertin technology 2. Remove the sample tube from the dry ice and immediately pipet 700 ul QIAzol Lysis Reagent into each sample tube. 3. Homogenize immediately on the TissueLyser for 2x10sec 5000 with 10sec break 4. Place the sample tube containing the homogenate on the benchtop at room temperature (15-25C) for 5 min. This step promotes dissociation of nucleoprotein complexes. 5. Add 140 ul chloroform and shake it vigorously for 15s. Thorough mixing is important for subsequent phase separation. 6. Place the sample tube on the benchtop at room temperature for 2-3 min. 7. Centrifuge at >6000g (max. 9000g for Precellys tubes) for 15 min at 4C. After centrifugation, the sample separates into 3 phases: an upper, colorless, aqueous phase containing RNA; a white interphase enriched with DNA; and a lower, red, organic phase containing proteins. For tissues with an especially high fat content, an additional, clear phase may be visible below the red, organic phase. The volume of the aqueous phase should be approximately 350 ul. 8. Transfer the upper aqueous phase to a new reaction tube. Centrifuge again if a little bit of the red organic phase was transferred accidentally. This is important because it will interfere with the downstream precipitation. 9. Add 1 Vol% of isopropanol and mix thoroughly. Incubate 15 min at RT 10. Centrifuge full-speed, 20 min, 4C. 11. Wash with 750 ul 75% EtOH 12. Centrifuge full-speed, 10 min, 4C 13. Allow pellet to air-dry 14. Dissolve pellet in 20-50 ul RNase-free water Step2, Turbo Dnase treat (Ambion AM1907) Volume 10ug Total RNA ~ 1/10th volume DNase buffer 10ul Turbo DNase enzyme 2ul Water to 100ul Incubate 37C 30 minutes (mix inactivation agent) 0.15 volume inactivation agent 15ul Step3, polyA+ enrichment (Promega PolyATtract(tm) mRNA Isolation Systems Z5310) Annealing of the probe Instructions are provided below to perform hybridization for 1-10ug of your total RNA sample with the Promega PolyATtract(tm) mRNA Isolation Systems III and IV. 1. To a sterile, RNase-free 1.5 mL microcentrifuge tube, bring 10 ug of total RNA to a final volume of 500 ul in RNase-free water 2. place the tube in a 65C water bath or heat block for 10 min. 3. add 3ul of the biotinylated-oligo(dT) probe and 13ul 20xSSC to the RNA. Mix gently and incubate at room temperature until completely colled. This should require about 10 min. Washing of streptavidin-paramagnetic particles 1. resuspent the manufacturer's provided streptavidin-paramagnetic particles (1 tube per sample), capture them by placing the tube on a magnetic stand 2. remove the supernatant 3. wash the streptavidin-paramagnetic particles three times with 0.5xSSC (300ul per wash), by gently flicking the bottom of the tube until the particles are resuspended, each time capturing them using the magnetic stand and carefully removing the supernatant 4. resuspend the streptavidin-paramagnetic particles in 100ul of 0.5xSSC Capture and washing of annealed oligo(dT)-mRNA hybrids 1. add the entire contents of the annealed oligo(dT)-mRNA hybrids (520 ul) to the washed streptavidin-paramagnetic particles 2. incubate at room temperature for 10 min, mix by gently inverting every minute 3. capture the streptavidin-paramagnetic particles using the magnetic stand and carefully remove the supernatant without disturbing the streptavidin-paramagnetic particles pellet 4. wash the streptavidin-paramagnetic particles four times with 0.1xSSC (300ul per wash) by gently flicking the bottom of the tube until the particles are resuspended, each time capturing them using the magnetic stand and carefully removing the supernatant. After the final wash, remove as much supernatant as possible. Elution of polyA+ RNA 1. resuspend the final streptavidin-paramagnetic particles in 100ul of RNase-free water and gently mix by flicking the tube 2. magnetically capture the streptavidin-paramagnetic particles and transfer the eluated polyA+ RNA in a RNase-free tube. 3. Repeat the capturing step by resuspending the streptavidin-paramagnetic particles pellet in 150ul of RNase-free water. Repeat the capturing step, pooling the eluate with the polyA+ RNA eluted before. Concentrating polyA+ RNA using ethanol precipitation To ensure recovery of polyA+ RNA, concentrate by using ethanol precipitation. Add the following components to polyA+ RNA: - 1 mL glycogen (20 mg/mL) - 1/10th sample (eluted RNA) volume (15 uL for this protocol) of 3 M sodium acetate - 2.5X sample volumes (375 uL for this protocol) of 100% ethanol Step4, Fractionate RNA (Ambion AM8740) Catalog #: AM8740 Ambion For fragmentation of 2-20 ug of RNA, the recommended reaction volume is 10 uL. 1. Bring the RNA sample volume to 9 uL with Nuclease-free Water (Ambion Cat #AM9938). 2. Add 1 uL of the 10X Fragmentation Buffer to the RNA sample. 3. Mix, spin briefly,and incubate at 70C for 15 min in a heating block, thermocycler, or water bath. 4. Add 1 uL of the Stop Solution, place it on ice until use, or store it at -80C. Concentrating fractionated polyA+ RNA using ethanol precipitation Add the following components to fractionated polyA+ RNA: - 1 mL glycogen (20 mg/mL) - 1/10th sample (eluted RNA) volume of 3 M sodium acetate - 2.5X sample volumes of 100% ethanol Step5, Synthesize the 1st strand cDNA 1. add 1 ul of random primers to 11.1 ul of fractionated polyA+ RNA 2. place the tube in a 65C PCR thermal cycler for 5 min, and chill on ice. 3. mix the following reagents in the order listed: 5x 1st strand buffer 4ul 100 mM DTT 2ul 25 mM dNTP mix 0.4 ul RNase OUT 0.5 ul 4. add the mixture to the PCR tube and mix well 5. heat the sample at 25C for 2 min 6. add 1ul SuperScript II to the sample and incubate in a thermal cycler: a. 25C for 10 min b. 42C for 50 min c. 70C for 15 min d. hold at 4C Step6, Synthesize the 2nd strand cDNA 1. add: 62.8ul of water to the 1st strand cDNA synthesis mix 10ul GEX second strand buffer 1.2ul 25mM dNTP mix 2. incubate on ice for 5 min 3. add 1ul of RNaseH and 5ul of DNA pol I 4. mix and incubate at 16C in a thermal cycler for 2.5h 5. follow the instructions in the QIAquick PCR Purification Kit to purify the sample and elute in 50ul of EB Step7, A. End Repair Pipett the following mix direct into PCR tubes and keep on ice. Fractionated poly A+ RNA 50.0 ul Water 25.0 ul T4 DNA Ligase buffer with 10 mM ATP 10.0 ul dNTP mix (each 10 mM) 4.0 ul T4 DNA polymerase 5.0 ul Klenow DNA polymerase 1.0 ul T4 PNK 5.0 ul Total 100.0 ul 1. Incubate 30 min at 20C in a thermal cycler. 2. Cleanup samples using the DNA Clean&Concentrator-5 kit, (Zymo research,USA), following the manufacturer's protocol. 3. Elute with 32 ul EB preheated to 50C. Chill on ice. Step8, Add A Bases Pipett the following mix direct into PCR tubes and keep on ice. DNA sample 32.0 ul Klenow buffer 5.0 ul dATP (1mM) 10.0 ul Klenow (3'-5' exo minus; 5 U/ul) 3.0 ul Total 50.0 ul 1. Incubate 30 min at 37C in a thermal cycler. 2. Cleanup samples using the DNA Clean&Concentrator-5 kit, (Zymo research,USA), following the manufacturer's protocol. 3. Elute with 8 ul EB preheated to 50C. Chill on ice. Step9, Ligate sequencing Adapters to DNA fragments Pipett the following mix in 1.5 ml tubes. DNA sample 8.0 ul DNA Ligase buffer 12.5 ul Adapter Oligo mix (from Illumina) 1.0 ul DNA ligase 2.5 ul Total 25.0 ul 1. Incubate 15 min at RT. (Requires Rapid DNA Ligase, else incubate 16 h at 16C. 2. Cleanup samples using the DNA Clean&Concentrator-5 kit, (Zymo research,USA), following the manufacturer's protocol. 3. Elute with 23 ul EB preheated to 50C. Chill on ice. Step10, Enrich Adapter-Modified DNA by PCR: Pipette the following mix direct into PCR tubes and keep on ice. DNA sample 23.0 ul Phusion DNA pol 2x master mix 25.0 ul PCR primer 1.0 1.0 ul PCR primer 2.0 1.0 ul Total 50.0 ul Run the PCR using the following program: Step1: 98C 30 sec Step2: 98C 10 sec Step3: 65C 30 sec Step4: 72C 30 sec Step5 GOTO Step2 14 times Step6: 72C 5 min Step7: 4C HOLD Step11, purify the RNAseq library 1. prepare a 2% agarose gel in 1xTAE 2. add 8ul of 6xDNA loading dye to the PCR-enriched DNA 3. loading: use 2ul of 100bp DNA ladder (with 6xDNA loading dye) and sample 4. run the gel at 120V for 60 min 5. excise the 200 bp (+/- 25 bp) region of a gel with a scalpel or gene catcher

text: adult

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

characteristic: title


characteristic: External Id

text: CRUK-CRI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Rhesus monkey

characteristic: description

text: adult

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male

text: mmlBlues


text: CRUK-CRI

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

text: hsaAmbion


characteristic: ENA-FIRST-PUBLIC

text: CRUK-CRI

text: public

text: ArrayExpress

text: Norway rat

text: adult

characteristic: developmental stage

text: Rattus norvegicus

text: Rattus norvegicus

text: liver

text: Rattus norvegicus

text: male


text: CRUK-CRI

text: public

characteristic: INSDC status

text: ArrayExpress

text: Rhesus monkey

text: adult

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male

characteristic: sex

text: mmlBob


text: CRUK-CRI

text: public

characteristic: SRA accession

text: ArrayExpress

text: dog

text: adult

text: Canis lupus familiaris

text: Canis familiaris

text: liver

text: Canis lupus familiaris

text: male


text: CRUK-CRI

text: public

characteristic: StrainOrLine

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: testes

text: Mus musculus

text: male


characteristic: ENA-LAST-UPDATE

text: CRUK-CRI

text: public

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: CRUK-CRI

text: public

text: ArrayExpress

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

characteristic: title


characteristic: External Id

text: CRUK-CRI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: CRUK-CRI

characteristic: INSDC first public

text: public

text: ArrayExpress

text: dog

characteristic: description

text: adult

text: Canis lupus familiaris

text: Canis familiaris

text: liver

text: Canis lupus familiaris

text: male


text: CRUK-CRI

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: gray short-tailed opossum

text: adult

text: Monodelphis domestica

text: Monodelphis domestica

text: liver

text: Monodelphis domestica

text: male


characteristic: ENA-FIRST-PUBLIC

text: CRUK-CRI

text: public

text: ArrayExpress

text: house mouse

text: adult

text: Mus musculus

characteristic: organism

text: Mus musculus

text: muscle

text: Mus musculus

text: male


text: CRUK-CRI

text: public

characteristic: INSDC status

text: ArrayExpress

text: Rhesus monkey

text: adult

characteristic: developmental stage

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male

text: mmlJoseph


characteristic: ENA-CHECKLIST

text: Faculty of medecine and pharmacy RABAT

text: public

characteristic: SRA accession

tag: Namespace:Faculty of medecine and pharmacy RABAT

text: Whole-Genome Shotgun Sequence of MDR Mycobacterium Tuberculosis MTB20_M isololated from Morocco

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: KI-BN

text: public

text: zebrafish

text: RNA extracted from zebrafish embryo at 50% epiboly stage

text: Danio rerio

text: Danio rerio

text: Mixed

characteristic: sex

text: Tuebingen

characteristic: strain

text: zebrafish embryo 50 epiboly


text: KI-BN

text: public

text: zebrafish

characteristic: common name

text: RNA extracted from zebrafish embryo at 1 cell stage

text: Danio rerio

text: Danio rerio

text: Mixed

text: Tuebingen

text: zebrafish embryo 1 cell

characteristic: title


characteristic: External Id

text: KI-BN

characteristic: INSDC center name

text: public

text: zebrafish

text: RNA extracted from zebrafish embryo at 16 cell stage

text: Danio rerio

text: Danio rerio

text: Mixed

text: Tuebingen

text: zebrafish embryo 16 cell


text: KI-BN

characteristic: INSDC first public

text: public

text: zebrafish

text: RNA extracted from zebrafish embryo at 512 cell stage

characteristic: description

text: Danio rerio

text: Danio rerio

text: Mixed

text: Tuebingen

text: zebrafish embryo 512 cell


text: SC

characteristic: INSDC last update

text: public

characteristic: StrainOrLine

text: Jackson laboratory

characteristic: biosourceprovider

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mouse tissues where obtained from 8 weeks old mice from the Jackson lab Maine USA. Total RNA extraction using trizol as per manufacturer instructions

text: adult

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Jackson laboratory

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: thymus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: Submitter Id

text: Jackson laboratory

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

text: Mus musculus

text: Mus musculus

text: heart

text: Mus musculus


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: Jackson laboratory

text: ArrayExpress

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: hippocampus

text: Mus musculus

characteristic: title


text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Jackson laboratory

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus


text: SC

text: public

characteristic: StrainOrLine

text: Jackson laboratory

characteristic: biosourceprovider

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 290

characteristic: sample description

text: Saccharomyces cerevisiae


text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 289

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: organism

text: Mat alpha, URA+ segregant 292

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 293

text: Saccharomyces cerevisiae


text: SC

text: public

characteristic: Submitter Id

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 296

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 295

text: Saccharomyces cerevisiae


characteristic: External Id

text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 379

text: Saccharomyces cerevisiae


text: SC

characteristic: INSDC center name

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 376

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 367

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 226

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 220

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 217

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 224

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 223

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 346

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 394

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 241

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 242

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 244

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 239

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 388

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 245

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 343

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 340

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 302

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 305

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 306

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 309

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 361

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 215

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 211

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 212

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 214

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 208

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 209

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 364

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 237

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 222

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 219

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 213

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 231

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 228

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 274

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 322

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 272

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 269

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 268

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 316

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 341

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 331

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 345

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 348

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 275

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 202

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 203

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 206

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 205

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 330

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 374

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 210

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 385

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 395

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 396

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 201

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 204

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 392

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 393

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 339

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 332

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 338

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 335

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 336

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 234

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 276

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 273

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 282

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 285

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 294

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 291

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 304

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 350

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 232

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 369

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 368

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 366

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 363

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 362

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 301

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 313

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 325

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 249

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 286

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 246

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 264

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 258

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 277

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 278

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 267

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 280

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 281

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 319

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 283

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat alpha, URA+ segregant 284

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 351

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 353

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 354

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 356

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 357

text: Saccharomyces cerevisiae


text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Mat a, LYS+ segregant 359

text: Saccharomyces cerevisiae