characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TATTCTACATGA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 34

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF BRITISH COLUMBIA

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

unit: m

characteristic: geographic location (depth)

unit: DD

text: mimarks-survey

text: CTGGTAAGTCCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 35

characteristic: pcr conditions

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TATGCTCTCTCA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 36

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: CCGCATGACCTA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 37

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TTCGACTAATAT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 38

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: TAATCATGTAAT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 39

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GTTGGGATCCTC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 40

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: GACGTCCCTCCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 41

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GTCTGACGGTCT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 42

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: TTCACGCGCCCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 43

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: ACATCAGGTCAC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 44

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: AATCTCTATAAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 45

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: CTTCTTCGCCCT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 46

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: TAGTGTCGGATC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 47

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GCATCGTCTGGT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 48

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: ATGCGCCCGTAT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 49

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GAGTCCGTTGCT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 50

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: CTGTACTTCTAA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 51

characteristic: pcr conditions

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GTCCCAGTCCCA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 52

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: TGTATGCTTCTA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 53

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GAGTTCCATTGG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 54

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: AATGACCTCGTG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 55

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGTTATTCTAGT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 56

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: TCCGACCCGATC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 57

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGCTATGTATGG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 58

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: TGGTTTCGAAGA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 59

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TCTTAAGATTTG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 60

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: CGGTAGTTGATC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 61

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory natural

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GACCACTGCTGT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 62

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: GGTCCTTCCCGA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 63

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

text: blade

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: CACATTCTATAA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 64

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: AAGGGTTAGTCT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 65

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: TCAAGCAATACG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 66

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: TAGGCGGTAGGC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 67

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: AAGTAGGAAGGA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 68

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: GAAGATCTATCG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 69

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: TCGATAAGTAAG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 70

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: CACTAGACCCAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 71

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: TCTCCTAGGCGC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 72

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: CTTATAGAGAAG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 73

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: AGTAATAACAAG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 74

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: CGTGTTATGTGG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 75

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GAGGCGCCATAG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 76

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus natural

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: TACACGCTGATG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 77

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Mastocarpus natural

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GTGGCGCATGGA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 78

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus natural

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: AGCCAGTCATAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 79

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Mastocarpus natural

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: TGGAATTCGGCT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 80

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus natural

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: ATACTGAGTGAA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 81

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GCCGCGGGATCA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 82

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: ACACTCATTACT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 83

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Mastocarpus laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GTTTGCTCGAGA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 84

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: GCGTTAACCCAA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 85

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Mastocarpus laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blade

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: GAACTCGCTATG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 86

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Mastocarpus laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: ATCGCCGCCTTG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 87

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory natural

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: CAGACCGGACGA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 88

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: GTAGGTGCTTAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 89

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

text: mimarks-survey

characteristic: investigation type

text: TAGACCGACTCC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 90

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: GTGTAATGTAGA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 91

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GGCCCTGTGGGC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 92

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: CAACTTAATGTT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 93

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GGAGGAGCAATA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 94

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: TCGTAGTAATGG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 95

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: GCAGAGAGGCTA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 96

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: aquarium

text: saline water aquarium

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: GGAATCCGATTA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 97

characteristic: pcr conditions

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: meristem

characteristic: body site

characteristic: collection date

text: aquarium

characteristic: environment (biome)

text: saline water aquarium

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

characteristic: host body product

text: mimarks-survey

characteristic: investigation type

text: GGACTTCCAGCT

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 98

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Nereocystis leutkeana laboratory

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

characteristic: Submitter Id

characteristic: adapters

text: meristem

text: aquarium

text: saline water aquarium

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

text: mimarks-survey

text: CGATATCAGTAG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 99

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nereocystis.Environmental.StarfishInner.4

text: blade

characteristic: body site

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

characteristic: host body product

text: mimarks-survey

characteristic: investigation type

text: CGGGTCCTCTTG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 100

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

text: Nereocystis.Environmental.StarfishInner.3

characteristic: Submitter Id

characteristic: adapters

text: blade

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

text: mimarks-survey

text: TAGGTAACCGAT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 101

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: Nereocystis leutkeana laboratory natural

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nereocystis.Environmental.StarfishInner.2

text: blade

characteristic: body site

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

characteristic: host body product

text: mimarks-survey

characteristic: investigation type

text: CCAGCGCTTCAC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 102

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

text: Nereocystis.Environmental.StarfishInner.1

characteristic: Submitter Id

characteristic: adapters

text: blade

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

text: mimarks-survey

text: GAGGCAAACGCC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 103

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nereocystis.Environmental.StarfishOuter3.2

text: AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT

text: blade

characteristic: body site

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

characteristic: host body product

text: mimarks-survey

characteristic: investigation type

text: TAACCACCAACG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 104

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

text: Nereocystis.Environmental.StarfishOuter4.3

characteristic: Submitter Id

characteristic: adapters

text: blade

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

text: mimarks-survey

text: GCTAGACACTAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 105

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Nereocystis.Environmental.StarfishOuter5.4

text: blade

characteristic: body site

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: alga

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mucus

characteristic: host body product

text: mimarks-survey

characteristic: investigation type

text: GTCGGGCCGGTA

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 106

text: FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT

characteristic: project name

text: swab

text: snap off in cryotube

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

characteristic: Submitter Id

characteristic: adapters

text: neritic epipelagic zone biome�

text: rocky shore

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: CGTTTATCCGTT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 107

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

characteristic: sample size

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TAGGCATGCTTG

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 108

characteristic: project name

text: bottle

text: filter

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

text: public

text: Nereocystis.Environmental.StarfishOuter2.1

characteristic: Submitter Id

characteristic: adapters

text: blade

characteristic: body site

text: neritic epipelagic zone biome�

text: rocky shore

text: alga

text: Canada

characteristic: geographic location (depth)

text: mucus

characteristic: host body product

text: mimarks-survey

text: ATCCATGAGCGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 109

characteristic: pcr conditions

characteristic: pcr primers

text: swab

characteristic: sample collection device or method

text: snap off in cryotube

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: neritic epipelagic zone biome�

characteristic: environment (biome)

text: rocky shore

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AATAGTCGTGAC

text: metagenome

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 110

characteristic: project name

text: bottle

text: filter

characteristic: sample size

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: environmental

text: 16s rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BRITISH COLUMBIA

text: public

characteristic: Submitter Id

characteristic: adapters

text: neritic epipelagic zone biome�

text: rocky shore

text: water

text: Canada

characteristic: geographic location (depth)

text: mimarks-survey

text: CATGAACAGTGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: metagenome

characteristic: organism

text: initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 111

characteristic: pcr conditions

characteristic: pcr primers

text: bottle

characteristic: sample collection device or method

text: filter

characteristic: sample material processing

text: metagenome

text: Illumina MiSeq

text: environmental

characteristic: specific host

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


text: Ptilinopus melanospilus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: black-naped fruit-dove

characteristic: common name

text: Ptilinopus melanospilus

text: Ptilinopus melanospilus

characteristic: subject id

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DTU-GE

text: public

characteristic: Submitter Id

text: DTU Bioinformatics

characteristic: broker name

text: NVRI

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

text: Poland

characteristic: geo loc name

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: not provided

characteristic: host health state

text: unidentified

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: Food

characteristic: isolation source host associated

text: Escherichia coli

characteristic: organism

text: Escherichia coli


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institute of Molecular Biology (IMB)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Institute of Molecular Biology (IMB)

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: The 6-well tissue culture plate was put on ice and media was removed from each well. Next, each well was washed with 1 ml of ice-cold PBS. Cells were then first detached through pipetting with 1 ml of ice-cold PBS and next collected by centrifugation at 500xg for 5 min at 4°C. The supernatant was subsequently removed and the cell pellet was either snap-frozen and stored or immediately subjected to nucleic acid extraction. HEK293T cells were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum at 37°C with 5% CO2. The RON minigene was cloned into the pcDNA 3.1 (+) vector (Invitrogen). The minigene construct contains a sequence of the MST1R gene including exon 11 together with the complete flanking introns and the constitutive exons 10 and 12 (chr3: 49,933,098 - 49,933,837, GRCh37/hg19). The RON minigene library comprises of approximately 6,000 minigenes with three to four mutations on average per minigene. Two wells of a 6-well tissue culture with 90% confluent cells were each transfected with 2 µg of a minigene library one day prior to harvesting using 20 µg polyethylenimine MW ~ 2500 transfection reagent (Polysciences, Inc.). RNA extraction was performed using the QIAGEN RNeasy plus kit according to the manufacturer’s instructions. 40 µg of total RNA was used for two rounds of subsequent poly(A)-selection using magnetic Dynabeads® Oligo (dT)25 from Thermo Fisher Scientific according to the manufacturer's instructions. 400 - 500 ng of the resulting poly(A) selected RNA was used for cDNA synthesis using the RevertAid reverse transcriptase from Thermo Fisher Scientific according to the manufacturer's instructions. The resulting cDNA was used as a template for PCR amplification via an emulsion PCR protocol as described by Williams et al., 2006. The following steps were then performed to clean the water-in-oil emulsion: First, the water phase was extracted by washing twice with 1 ml of water-saturated diethyl ether. Second, residual solvent from the broken emulsion was removed by centrifugation under vacuum for 12 min at 30°C.Finally, PCR products were purified using 2 volumes of Agencourt® AMPure® XP beads from Beckman Coulter according to the maufacturer's instructions.

characteristic: description

text: late embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: pool of minigene library MST1R constructs

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: CBMSO

text: public

characteristic: Submitter Id

text: Check the genomic profile of colonies grown from an horizontal gene transfer event through a cell-to-cell conjugation like process between two different Thermus thermophilus strains. Forward and reverse reads that are unpaired, the reverse or forware read was lost during trimming.

text: Thermus thermophilus

characteristic: organism

text: Thermus thermophilus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CBMSO

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Check the genomic profile of colonies grown from an horizontal gene transfer event through a cell-to-cell conjugation like process between two different Thermus thermophilus strains

text: Thermus thermophilus

text: Thermus thermophilus


text: CBMSO

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Check the genomic profile of colonies grown from an horizontal gene transfer event through a cell-to-cell conjugation like process between two different Thermus thermophilus strains. Single Reads( forward and reverse reads that are unpaired, the reverse or forware read was lost during trimming)

text: Thermus thermophilus

text: Thermus thermophilus


text: PRIF UK

text: public

tag: Namespace:PRIF UK

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: UNIVERSITY OF ZURICH

text: public

tag: Namespace:UNIVERSITY OF ZURICH

text: New genome assembly of the Blumeria graminis f. sp. tritici isolate 96224. Resequenced loci (Bourras et al. 2015, Parlange et al. 2015, Praz et al. 2017 and unpublished data) were also integrated into the previous assembly of the Blumeria gramini f. sp. tritici isolate 96224 (PRJNA183607). We also used new Illumina sequencing data to correct for SNPs in the assembly.

characteristic: description

characteristic: isolate

text: Blumeria graminis f. sp. tritici 96224

text: Blumeria graminis f. sp. tritici 96224

text: spore

characteristic: tissue type

text: Blumeria graminis f. sp. tritici genome assembly v2.1


text: Biocentre, University Wuerzburg

text: public

characteristic: collection date

text: uncultured fungus

text: forage material

text: grassland

characteristic: environment (biome)

text: grassland biome

characteristic: environment (feature)

text: forage material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: metagenome

characteristic: investigation type

text: uncultured fungus

text: plant-associated

characteristic: plant-associated environmental package

text: Defense

characteristic: project name

text: uncultured fungus

text: Illumina

characteristic: sequencing method

text: ITS

characteristic: target gene

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

characteristic: Submitter Id

text: Salmonella enterica subsp. enterica serovar Derby

characteristic: common name

text: Germany

text: Salmonella enterica

text: animal

characteristic: isolation source

characteristic: organism

text: Derby

characteristic: serovar

text: enterica

characteristic: sub species

text: ENGAGE-project Salmonella strains from BfR

characteristic: title


text: FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: public

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: Salmonella enterica

characteristic: isolate

text: environment

text: Derby

text: enterica


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: Germany

text: Salmonella enterica

text: animal

text: Derby

text: enterica


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Germany

text: Salmonella enterica

text: food

text: Derby

text: enterica