characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF GRONINGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF GRONINGEN

characteristic: Submitter Id

characteristic: collection date

text: marine biome

text: bay

text: water

text: Yes

characteristic: environmental sample

characteristic: further details

text: Antarctica

text: uncultured eukaryote

characteristic: organism

text: V4F (5-CCA GCA SCY GCG GTA ATT CC-3); V4RB (5-ACT TTC GTT CTT GAT YRR-3)

characteristic: pcr primers

text: Niskin and hand winch

characteristic: sample collection device or method

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

text: Sequences Rothera

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Environmental and Applied Microband Applied Microb

text: origin arc

characteristic: description

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: culture


characteristic: External Id

text: Environmental and Applied Microband Applied Microb

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 1st arc ck

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 1st arc exp

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: origin bac

text: prokaryote enrichment culture

characteristic: organism

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 1st bac exp

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 1st bac ck

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 2nd arc exp

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 2nd arc ck

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 2nd bac exp

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: public

text: 2nd bac ck

text: prokaryote enrichment culture

text: prokaryote enrichment culture

text: oil degradation of G35


text: Centre de Bioinformatique de Bordeaux

text: public

tag: Namespace:Centre de Bioinformatique de Bordeaux

text: human

characteristic: common name

text: Replicate 1 of angiogenic tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma

characteristic: tissue type


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 1 of angiogenic tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 2 of angiogenic tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 2 of angiogenic tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 3 of angiogenic tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 3 of angiogenic tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 4 of angiogenic tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 4 of angiogenic tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 1 of invasive tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 1 of invasive tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 2 of invasive tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 2 of invasive tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 3 of invasive tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 3 of invasive tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: human

text: Replicate 4 of invasive tumor Human reads

text: Homo sapiens

text: Homo sapiens

text: Glioma


text: Centre de Bioinformatique de Bordeaux

text: public

text: house mouse

text: Replicate 4 of invasive tumor Mouse reads

text: Mus musculus

text: Mus musculus

text: Glioma


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


text: public

tag: Namespace:Sanger Institute / Karolinska Institutet

text: 6 to 8

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T-helper 2 cell

characteristic: cell type

text: house mouse

text: Protocols: CD4+CD62L+ naive T cells were purified from spleens of wild-type C57BL/6JAX adult (6 - 8 weeks) mice using the CD4+CD62L+ T Cell Isolation Kit II (Miltenyii #130-093-227). Cell culture plates were coated with anti-CD3e antibody (1 ug/ml, eBioscences #16-0031-81) in 1X DPBS (Gibco) at 4°C overnight. Purified naive T cells were seeded at a concentration of 1 M cells/ml on the coated plates in IMDM (Gibco) with 10% heat-inactivated FBS (Sigma), supplied with 5 ug/ml anti-CD28 (eBioscience #16-0281-86) with (Th2) or without (Th0) 10 ng/ml mouse recombinant IL-4 (R&D Systems #404-ML-050). Cells were cultured in plates for up to 72 hours. Total RNA was purified by Qiagen RNeasy Mini Kit according to manufacturer's instruction, and concentration was determined by a Nanodrop. A total of 500 ng RNA was used to prepare sequencing libraries using KAPA Stranded mRNA-seq Kit (KAPA #07962193001) according to manufacturer's instructions. Sequencing was performed on an Illumina HiSeq 2000 (125bp PE, v4 chemistry). ChIPmentation was used to investigate the TF binding sites. 1 million cells from each sample were crosslinked in 1% HCHO (prepared in 1X DPBS) at room temperature for 10 minutes, and HCHO was quenched by the addition of glycine at a final concentration of 0.125 M. Cells were pelleted at 4°C at 2000 x g, washed with ice-cold 1X DPBS twice, and snapped frozen in liquid nitrogen. The cell pellets were stored in -80°C until the experiments were performed. ChIPmentation was performed according to the version 1.0 of the published protocol (http://www.medical-epigenomics.org/papers/schmidl2015/) with some modifications at the ChIP stage.Briefly, cell pellets were thawed on ice, and lysed in 300 ul ChIP Lysis Buffer I (50 mM HEPES.KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, pH 8.0, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100) on ice for 10 minutes. Then cells were pelleted at 4°C at 2000 x g for 5 minutes, and washed by 300 ul ChIP Lysis Buffer II (10 mM Tris.Cl, pH 8.0, 200 mM NaCl, 1 mM EDTA, pH 8.0, 0.5 mM EGTA, pH 8.0), and pelleted again at 4°C at 2000 x g for 5 minutes. Nuclei were resuspended in 300 ul ChIP Lysis Buffer III (10 mM Tris.Cl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% Sodium Deoxycholate, 0.5% N-Lauroylsarcosine). Chromatin was sonicated using Bioruptor Pico (Diagenode) with 30 seconds ON/30 seconds OFF for 10 cycles. 30 ul 10% Triton X-100 was added into each sonicated chromatin, and insoluble chromatin was pelleted at 16,100 x g at 4°C for 10 minutes. 1 ul supernatant was taken as input control. The rest of the supernatant was incubated with 10 ul Protein A Dynabeads (Invitrogen) pre-bound with 1 ug anti-FLAG in a rotating platform in a cold room overnight. Each immunoprecipitation (IP) was washed with 500 ul RIPA Buffer (50 mM HEPES.KOH, pH 7.5, 500 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% Sodium Deoxycholate, check components) for 3 times. Then, each IP was washed with 500 ul 10 mM Tris, pH 8.0 twice, and resuspended in 30 ul tagmentation reaction mix (10 mM Tris.Cl, pH 8.0, 5 mM Mg2Cl, 1 ul TDE1 (Nextera)). Then, the tagmentation reaction was put on a thermomixer at 37°C for 10 minutes at 800 rpm shaking. After the tagmentation reaction, each IP was washed sequentially with 500 ul RIPA Buffer twice, and 1X TE NaCl (10 mM Tris.Cl, pH 8.0, 1 mM EDTA, pH 8.0, 50 mM NaCl) once. Elution and reverse-crosslinking were done by resuspending the beads with 100 ul ChIP Elution Buffer (50 mM Tris.Cl, pH 8.0, 10 mM EDTA, pH 8.0, 1% SDS) on a thermomixer at 65°C overnight, 1,400 rpm. DNA was purified by MinElute PCR Purification Kit (QIAGEN, #28004 and eluted in 12.5 ul Buffer EB (QIAGEN kit, #28004), which yielded ~10 ul ChIPed DNA. The library preparation reactions contained the following: 10 ul purified DNA (from above) 2.5 ul PCR Primer Cocktails (Nextera DNA Library Preparation Kit, Illumina #FC-121-1030) 2.5 ul N5xx (Nextera Index Kit , Illumina #FC-121-1012) 2.5 ul N7xx (Nextera index kit , Illumina #FC-121-1012) 7.5 ul NPM PCR Master Mix (Nextera DNA Library Preparation Kit, Illumina #FC-121-1030)PCR was set up as follows: 72°C, 5 mins 98°C, 2 mins [98°C, 10 secs, 63°C, 30 secs, 72°C, 20 secs] x 12 10°C holdThe amplified libraries were purified by double AmpureXP beads purification: first with 0.5X bead ratio, keep supernatant, second with 1.4X bead ratio, keep bound DNA. Elution was done in 20 ul Buffer EB (QIAGEN).1 ul of library was run on an Agilent Bioanalyzer to see the size distribution

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: anti-CD28 (5 microgram per milliliter) and interleukin-4 (10 nanogram per milliliter)

characteristic: stimulus

characteristic: strain

characteristic: title

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute / Karolinska Institutet

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 6 to 8

text: ArrayExpress

text: T-helper 2 cell

text: house mouse

characteristic: description

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus


text: Georgia State University

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Georgia State University

characteristic: Submitter Id

characteristic: collection date

text: house mouse

characteristic: common name

text: JunProject

text: terrestrial biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Mus musculus

characteristic: organism

text: JunProject

characteristic: project name

text: Mus musculus

text: Illumina MiSeq

characteristic: sequencing method

text: JunProject


text: iPSC

text: mixed

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: Mixed

characteristic: gender

text: Homo sapiens

text: Homo sapiens

characteristic: subject id

characteristic: title


text: OX

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: broker name

characteristic: description

text: Neisseria meningitidis

text: Neisseria meningitidis


text: OX

text: public

text: Neisseria subflava

characteristic: organism

text: Neisseria subflava


text: OX

text: public

text: Neisseria lactamica

text: Neisseria lactamica


text: OX

text: public

text: Neisseria cinerea

text: Neisseria cinerea


text: PERIO-UKM

text: public

text: human oral metagenome

text: human oral metagenome

text: human oral metataxonomics


E-MTAB-6283:IgG exp1

text: public

text: E-MTAB-6283:IgG exp1

tag: Namespace:Department of Molecular Neuroscience, UCL Institute of Neurology

characteristic: age

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: Whole brain was prepped from mice at P0 and quick frozen in liquid nitrogen. Tissue samples were later thawed and dissociated in PBS, and crosslinked at 150mJ/cm2 in suspension. Cells were pelleted, and snap-frozen in liquid nitrogen until cell lysis. Cell were lysed in 1 ml of iCLIP lysis buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate) buffer, and the remaining DNA/nuclear pellet was sonicated for 3x 10seconds. Then we added 2µl TurboDNase and incubated the lysate for 3 minutes at 37°C. Insoluble pellet was collected and dissolved in 30µl MSB buffer ((50mM Tris-HCl pH 7.4, 100mM NaH2PO4, 7M UREA, 1mM DTT) and sonicated again. Lysate was topped up to 2ml to dilute the urea and incubated with 4 U/ml of RNase I and 2 l antiRNase (1/1000, AM2690, Thermo Fisher) at 37C for 3 min, and centrifuged. We took care to prepare the initial dilution of RNase in water, since we found that RNase I gradually loses its activity when diluted in the lysis buffer. For immunoprecipitation, 80ul of Protein A Dynabeads were washed in iCLIP lysis buffer (as above), and incubated with IgG control or rabbit anti MATR3 antibody (5ul, GTX47279). The rest of the protocol was identical to the published protocol (Huppertz I, Attig J, D'Ambrogio A, Easton LE, Sibley CR, Sugimoto Y, Tajnik M, Knig J, Ule J: iCLIP: protein-RNA interactions at nucleotide resolution. Methods 2014, 65:274-287.) Following the iCLIP protocol, RNA was retrieved from a nitrocellulose membrane after immunoprecipitation of the protein of interest and 32P labelling. After RNA elution, construction of Illumina compatible libraries was done as described in Huppertz et al, 2014.

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

text: Mus musculus

characteristic: strain

text: IgG exp1


E-MTAB-6283:IgG exp2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Molecular Neuroscience, UCL Institute of Neurology

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6283:IgG exp2

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: IgG exp2


E-MTAB-6283:MATR3 exp1 repl1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6283:MATR3 exp1 repl1

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: MATR3 exp1 repl1

characteristic: title


E-MTAB-6283:MATR3 exp1 repl2

text: public

text: E-MTAB-6283:MATR3 exp1 repl2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: normal

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: MATR3 exp1 repl2


E-MTAB-6283:MATR3 exp2 repl3

text: public

text: E-MTAB-6283:MATR3 exp2 repl3

characteristic: age

text: ArrayExpress

text: house mouse

characteristic: common name

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: MATR3 exp2 repl3


E-MTAB-6283:MATR3 exp2 repl4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6283:MATR3 exp2 repl4

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

text: Mus musculus

characteristic: strain

text: MATR3 exp2 repl4


E-MTAB-6283:MATR3 exp2 repl5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6283:MATR3 exp2 repl5

text: ArrayExpress

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: MATR3 exp2 repl5


text: URMITE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: sputum

characteristic: isolation source

text: Mycobacterium sp. 3519A

text: Mycobacterium sp. 3519A

text: Draft genome sequencing of Mycobacterium sp. 3519A

characteristic: title


text: Alexander

text: public

characteristic: Submitter Id

text: Lactococcus lactis

characteristic: organism

text: Lactococcus lactis

text: Lactococcus lactis from undefined mesophilic starter cultures


text: Alexander

text: public

text: Lactococcus lactis subsp. lactis

text: Lactococcus lactis subsp. lactis

text: Lactococcus lactis subsp. lactis CF102


text: Alexander

text: public

text: Lactococcus lactis subsp. cremoris

text: Lactococcus lactis subsp. cremoris

text: Lactococcus lactis subsp. cremoris CF107


text: Alexander

text: public

text: Lactococcus lactis subsp. cremoris

text: Lactococcus lactis subsp. cremoris

text: Lactococcus lactis subsp. cremoris CF108


text: Alexander

text: public

text: Lactococcus lactis subsp. lactis

text: Lactococcus lactis subsp. lactis

text: Lactococcus lactis subsp. lactis CF109


text: UNIVERSITY OF GENEVA

text: public

tag: Namespace:UNIVERSITY OF GENEVA

text: RNA extracted from P.berghei sporozoites - Replicate A

characteristic: description

text: Plasmodium berghei ANKA

text: Plasmodium berghei ANKA


text: UNIVERSITY OF GENEVA

text: public

text: RNA extracted from P.berghei sporozoites - Replicate B

text: Plasmodium berghei ANKA

text: Plasmodium berghei ANKA


text: URMITE

text: public

text: sputum

text: Mycobacterium sp. 4858

text: Mycobacterium sp. 4858

text: Draft genome sequencing of Mycobacterium sp. 4858


text: female

characteristic: ArrayExpress-SEX

text: Teladorsagia circumcincta

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Teladorsagia circumcincta

characteristic: common name

text: Female

characteristic: gender

text: Teladorsagia circumcincta

text: Teladorsagia circumcincta

characteristic: subject id

text: Teladorsagia circumcincta


text: female

text: Haemonchus contortus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: barber pole worm

text: Female

text: Haemonchus contortus

text: Haemonchus contortus

text: Haemonchus contortus


characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: collection date

text: Collaboration project between CDC, Institut Pasteur and the Norwegian Institute of Public Health (FHI). WGS of N meningitidis from Africa.

text: Togo

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: CSF

text: Neisseria meningitidis

text: Neisseria meningitidis

text: W

characteristic: serovar


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: description

text: Nigeria

text: diseased

text: Homo sapiens

text: CSF

characteristic: isolation source

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis

text: C

characteristic: title


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


text: Plasmodium vivax

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax


Innovator

text: James Hutton Institute

characteristic: INSDC last update

text: public

text: Innovator

tag: Namespace:James Hutton Institute

text: potato

text: Potato cultivar Innovator whole genome shotgun reads

text: Solanum tuberosum

text: Solanum tuberosum

text: Innovator WGS reads


E-MTAB-6282:Sample 1

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 1

tag: Namespace:Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

text: ArrayExpress

characteristic: broker name

text: poinsettia

text: Lilo Red

characteristic: cultivar

text: Protocols: Cuttings with two small leaves were taken from Euphorbia pulcherrima Willd ex Klotzsch. (Poinsettia) ‘Lilo Red’ (Ecke Ranch, California, USA) mother plants grown under long day conditions (LD) (16 h light from High Pressure Sodium (HPS lamps) at 150 molm-2s-1, 22°C day/ 20°C night, 70% Relative Humidity (RH)).The freeze-dried material on the slide PET membranes were placed on the laser microdissection microscope (Leica model 6000, Microsystems, Germany), the objective 6,3X was used to dissect the three different tissues for further RNA isolation: distal, abscission zone (AZ) and proximal. The software Laser microdissection software V6.7.2.4295 (Leica Microsystems, Germany), was used to control the microscope and select the average conditions of the laser such as the aperture 45, laser power 60 and speed 20. The mass of the tissues was calculated using the volume obtained. The microdissected tissues were collected inside a cap of a 0,6 ml RNAse free microcentrifuge tube, where they fell by gravity. The RNA isolation from the microdissected tissues was performed using the Ambion RNAqueous®- Micro kit (Ambion Inc., Austin, TX,Country??) following supplier instructions with small modifications as the use of a tip to crash the material inside the tube in presence of the lysis buffer. The protocol of isolation was followed by a 20 minutes DNAse treatment according to supplier instructions. The RNA was resuspended in 20  of nucleases free water, purified by LiCl-EtOH precipitation and stored at -80°C. The concentration and quality of the RNA was analysed using the Agilent 2100 Bioanalyzer (company, country) and the RNA 6000 Pico Kit chip (Agilent Technologies, CA, USA), following manufacturer instructions. The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: flower bud abscission zone

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 1


E-MTAB-6282:Sample 10

text: Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: description

text: long day length regimen

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 10

characteristic: title


E-MTAB-6282:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6282:Sample 11

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 11


E-MTAB-6282:Sample 12

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6282:Sample 12

text: ArrayExpress

text: poinsettia

characteristic: common name

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 12


E-MTAB-6282:Sample 2

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 2

text: ArrayExpress

characteristic: broker name

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 2


E-MTAB-6282:Sample 3

text: public

text: E-MTAB-6282:Sample 3

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: cultivar

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 3


E-MTAB-6282:Sample 4

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 4


E-MTAB-6282:Sample 5

text: public

text: E-MTAB-6282:Sample 5

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: description

text: long day length regimen

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 5

characteristic: title


E-MTAB-6282:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-6282:Sample 6

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 6


E-MTAB-6282:Sample 7

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6282:Sample 7

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 7


E-MTAB-6282:Sample 8

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 8

text: ArrayExpress

text: poinsettia

characteristic: common name

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 8


E-MTAB-6282:Sample 9

text: public

text: E-MTAB-6282:Sample 9

text: ArrayExpress

characteristic: broker name

text: poinsettia

text: Lilo Red

characteristic: cultivar

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: flower bud abscission zone

text: Euphorbia pulcherrima

text: Sample 9


E-MTAB-6282:Sample 13

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 13

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 13


E-MTAB-6282:Sample 14

text: public

text: E-MTAB-6282:Sample 14

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: description

text: long day length regimen

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 14


E-MTAB-6282:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6282:Sample 15

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 15

characteristic: title


E-MTAB-6282:Sample 16

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6282:Sample 16

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 16


E-MTAB-6282:Sample 17

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 17

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 17


E-MTAB-6282:Sample 18

text: public

text: E-MTAB-6282:Sample 18

text: ArrayExpress

characteristic: broker name

text: poinsettia

characteristic: common name

text: Lilo Red

characteristic: cultivar

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 18


E-MTAB-6282:Sample 19

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 19


E-MTAB-6282:Sample 20

text: public

text: E-MTAB-6282:Sample 20

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: description

text: long day length regimen

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 20


E-MTAB-6282:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6282:Sample 21

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 21


E-MTAB-6282:Sample 22

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6282:Sample 22

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 22

characteristic: title


E-MTAB-6282:Sample 23

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6282:Sample 23

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 23


E-MTAB-6282:Sample 24

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 24

text: ArrayExpress

characteristic: broker name

text: poinsettia

characteristic: common name

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: distal flower bud

text: Euphorbia pulcherrima

text: Sample 24


E-MTAB-6282:Sample 25

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: cultivar

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 25


E-MTAB-6282:Sample 26

text: public

text: E-MTAB-6282:Sample 26

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: description

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 26


E-MTAB-6282:Sample 27

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6282:Sample 27

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 27


E-MTAB-6282:Sample 28

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6282:Sample 28

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 28


E-MTAB-6282:Sample 29

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6282:Sample 29

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 29

characteristic: title


E-MTAB-6282:Sample 30

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 30

text: ArrayExpress

characteristic: broker name

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 30


E-MTAB-6282:Sample 31

text: public

characteristic: SRA accession

text: E-MTAB-6282:Sample 31

characteristic: Submitter Id

text: ArrayExpress

text: poinsettia

characteristic: common name

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 31


E-MTAB-6282:Sample 32

text: public

text: E-MTAB-6282:Sample 32

text: ArrayExpress

text: poinsettia

text: Lilo Red

characteristic: cultivar

characteristic: description

text: long day length regimen

characteristic: growth condition

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 32


E-MTAB-6282:Sample 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6282:Sample 33

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

characteristic: organism

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 33


E-MTAB-6282:Sample 34

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6282:Sample 34

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

characteristic: organism part

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 34


E-MTAB-6282:Sample 35

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6282:Sample 35

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

characteristic: sampling site

text: Euphorbia pulcherrima

text: Sample 35


E-MTAB-6282:Sample 36

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6282:Sample 36

text: ArrayExpress

text: poinsettia

text: Lilo Red

text: long day length regimen

text: Euphorbia pulcherrima

text: Euphorbia pulcherrima

text: flower bud

text: proximal flower bud

text: Euphorbia pulcherrima

text: Sample 36

characteristic: title


text: Plasmodium vivax

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax

characteristic: subject id


E-MTAB-5612:RNA-IP ZC3H32 Elu 1

text: ZMBH - University of Heidelberg

text: public

text: E-MTAB-5612:RNA-IP ZC3H32 Elu 1

tag: Namespace:ZMBH - University of Heidelberg

text: ArrayExpress

characteristic: broker name

text: Protocols: RNA IP (Flow_Through or Elute) Trifast follow rRNA depletion by RNAse-H treatment Illumina HiSeq

characteristic: description

text: bloodstream form

characteristic: developmental stage

text: expression of ZC3H32 with N-terminal TAP tag in ZC3H32 knockout background

characteristic: genotype

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 Elu 1


E-MTAB-5612:RNA-IP ZC3H32 Elu 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ZMBH - University of Heidelberg

text: public

text: E-MTAB-5612:RNA-IP ZC3H32 Elu 2

text: ArrayExpress

text: bloodstream form

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 Elu 2


E-MTAB-5612:RNA-IP ZC3H32 Elu 3 UV

characteristic: External Id

text: ZMBH - University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5612:RNA-IP ZC3H32 Elu 3 UV

text: ArrayExpress

text: bloodstream form

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 Elu 3 UV


E-MTAB-5612:RNA-IP ZC3H32 FT 1

text: ZMBH - University of Heidelberg

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5612:RNA-IP ZC3H32 FT 1

text: ArrayExpress

text: bloodstream form

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 FT 1


E-MTAB-5612:RNA-IP ZC3H32 FT 2

text: ZMBH - University of Heidelberg

text: public

text: E-MTAB-5612:RNA-IP ZC3H32 FT 2

text: ArrayExpress

text: bloodstream form

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 FT 2

characteristic: title


E-MTAB-5612:RNA-IP ZC3H32 FT 3 UV

text: ZMBH - University of Heidelberg

text: public

characteristic: SRA accession

text: E-MTAB-5612:RNA-IP ZC3H32 FT 3 UV

text: ArrayExpress

text: bloodstream form

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP ZC3H32 FT 3 UV


text: UPSC

text: public

characteristic: Submitter Id

text: thale cress

characteristic: common name

text: H3K4 Col0 replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 Col0 replicate A


text: UPSC

text: public

text: thale cress

text: H3K4 Col0 replicate B

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 Col0 replicate B


text: UPSC

text: public

text: thale cress

text: H3K4 Col0 replicate C

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 Col0 replicate C


text: UPSC

text: public

text: thale cress

text: H3K4 hub replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 hub replicate A


text: UPSC

text: public

text: thale cress

text: H3K4 hub replicate B

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 hub replicate B


text: UPSC

text: public

text: thale cress

text: H3K4 hub replicate C

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 hub replicate C


text: UPSC

text: public

text: thale cress

text: H3K4 prn replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 prn replicate A


text: UPSC

text: public

text: thale cress

text: H3K4 prn replicate B

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K4 prn replicate B


text: UPSC

text: public

text: thale cress

text: H3K36 Col0 replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 Col0 replicate A


text: UPSC

text: public

text: thale cress

text: H3K36 Col0 replicate B

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 Col0 replicate B


text: UPSC

text: public

text: thale cress

text: H3K36 Col0 replicate C

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 Col0 replicate C


text: UPSC

text: public

text: thale cress

text: H3K36 hub replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 hub replicate A


text: UPSC

text: public

text: thale cress

text: H3K36 hub replicate B

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 hub replicate B


text: UPSC

text: public

text: thale cress

text: H3K36 hub replicate C

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 hub replicate C


text: UPSC

text: public

text: thale cress

text: H3K36 prn replicate A

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 prn replicate A


text: UPSC

text: public

text: thale cress

text: H3K36 prn replicate C

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: H3K36 prn replicate C


text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: For modified iCLIP experiment, HEK293T cells were grown on 10 cm 2 dishes, incubated for 8 h with 100 M 4SU and crosslinked with 2x 400mJ/cm 2 365nm UV light. Protein A Dynabeads were used for immunoprecipitations (IP). 80 l of beads were washed in iCLIP lysis buffer (50mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate). For the preparation of the cell lysate, 2 million cells were lysed in 1 ml of iCLIP lysis buffer, and the remaining cell pellet was dissolved in 25uL MSB lysis buffer (50mM Tris-HCl pH 7.4, 100mM NaH2PO4, 7M UREA, 1mM DTT). After the pellet had dissolved, the mixture was diluted with CLIP lysis buffer to 1000ul and an additional centrifugation was performed. Lysates were pooled (2ml total volume) and 1.5ml incubated with 4 U/ml of RNase I and 2 l antiRNase (1/1000, AM2690, Thermo Fisher) at 37C for 3 min, and centrifuged. We took care to prepare the initial dilution of RNase in water, since we found that RNase I gradually loses its activity when diluted in the lysis buffer. 1.5 ml of the supernatant was then added to the beads and incubated at 4C for 4 h. The rest of the protocol was identical to the published protocol (Huppertz et al.). Huppertz I, Attig J, D'Ambrogio A, Easton LE, Sibley CR, Sugimoto Y, Tajnik M, Knig J, Ule J: iCLIP: protein-RNA interactions at nucleotide resolution. Methods 2014, 65:274-287. The anti-PTBP1 used is a non-commercial rabbit polyclonal antibody generated by a co-author. The reference is: Spellman R, Llorian M, Smith CW (2007) Crossregulation and functional redundancy between the splicing regulator PTB and its paralogs nPTB and ROD1. Mol Cell 27: 420 - 434 PMID: 17679092 Following the iCLIP protocol, RNA was retrieved from a nitrocellulose membrane after immunoprecipitation of the protein of interest and 32P labelling. After RNA elution, construction of Illumina compatible libraries was done as described in Huppertz et al, 2014.

text: embryo

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens


E-MTAB-6287:dKD2 rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6287:dKD2 rep1

tag: Namespace:Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London, WC1N 3BG, UK

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: Protocols: HeLa cells were grown in antibiotic free medium, and siRNAs delivered with Lipofectamin RNAiMax. Cells were collected 72 hours post transfection. Cells were fractionated into nuclear and cytoplasmic fractions with appropriate detergent conditions, and RNA was isolated from the nuclear pellet with Trizol and RNA isolation columns from Zymogen. RNA was treated with DNase I (Ambion) and converted to DNA libraries with the Lexogen QuantSeq kit (Lexogen; 1/2 with the forward and 1/2 with the reverse library kit).

characteristic: description

text: cervical adenocarcinoma

text: nuclear RNA

characteristic: fraction

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: dKD2 rep1


E-MTAB-6287:dKD2 rep2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London, WC1N 3BG, UK

characteristic: INSDC center name

text: public

text: E-MTAB-6287:dKD2 rep2

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: dKD2 rep2


E-MTAB-6287:dKD2 rep3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6287:dKD2 rep3

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: dKD2 rep3

characteristic: title


E-MTAB-6287:dKD2 rep4

text: public

characteristic: SRA accession

text: E-MTAB-6287:dKD2 rep4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: HeLa

text: epithelial cell

text: human

characteristic: common name

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: dKD2 rep4


E-MTAB-6287:siCtrl rep1

text: public

text: E-MTAB-6287:siCtrl rep1

text: ArrayExpress

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: cervical adenocarcinoma

characteristic: disease

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens

text: siCtrl rep1


E-MTAB-6287:siCtrl rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6287:siCtrl rep2

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

characteristic: description

text: cervical adenocarcinoma

text: nuclear RNA

characteristic: fraction

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siCtrl rep2


E-MTAB-6287:siCtrl rep3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6287:siCtrl rep3

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siCtrl rep3


E-MTAB-6287:siCtrl rep4

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6287:siCtrl rep4

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siCtrl rep4

characteristic: title


E-MTAB-6287:siMATR3 #1 rep1

text: public

characteristic: SRA accession

text: E-MTAB-6287:siMATR3 #1 rep1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: HeLa

text: epithelial cell

text: human

characteristic: common name

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #1 rep1


E-MTAB-6287:siMATR3 #1 rep2

text: public

text: E-MTAB-6287:siMATR3 #1 rep2

text: ArrayExpress

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: cervical adenocarcinoma

characteristic: disease

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens

text: siMATR3 #1 rep2


E-MTAB-6287:siMATR3 #1 rep3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6287:siMATR3 #1 rep3

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

characteristic: description

text: cervical adenocarcinoma

text: nuclear RNA

characteristic: fraction

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #1 rep3


E-MTAB-6287:siMATR3 #1 rep4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6287:siMATR3 #1 rep4

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #1 rep4


E-MTAB-6287:siMATR3 #2 rep1

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6287:siMATR3 #2 rep1

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #2 rep1

characteristic: title


E-MTAB-6287:siMATR3 #2 rep2

text: public

characteristic: SRA accession

text: E-MTAB-6287:siMATR3 #2 rep2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: HeLa

text: epithelial cell

text: human

characteristic: common name

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #2 rep2


E-MTAB-6287:siMATR3 #2 rep3

text: public

text: E-MTAB-6287:siMATR3 #2 rep3

text: ArrayExpress

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: cervical adenocarcinoma

characteristic: disease

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens

text: siMATR3 #2 rep3


E-MTAB-6287:siMATR3 #2 rep4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6287:siMATR3 #2 rep4

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

characteristic: description

text: cervical adenocarcinoma

text: nuclear RNA

characteristic: fraction

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siMATR3 #2 rep4


E-MTAB-6287:siPTBP1/2 rep1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6287:siPTBP1/2 rep1

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siPTBP1/2 rep1


E-MTAB-6287:siPTBP1/2 rep2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6287:siPTBP1/2 rep2

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siPTBP1/2 rep2

characteristic: title


E-MTAB-6287:siPTBP1/2 rep3

text: public

characteristic: SRA accession

text: E-MTAB-6287:siPTBP1/2 rep3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: HeLa

text: epithelial cell

text: human

characteristic: common name

text: cervical adenocarcinoma

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

text: siPTBP1/2 rep3


E-MTAB-6287:siPTBP1/2 rep4

text: public

text: E-MTAB-6287:siPTBP1/2 rep4

text: ArrayExpress

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: cervical adenocarcinoma

characteristic: disease

text: nuclear RNA

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens

text: siPTBP1/2 rep4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: URMITE

text: public

text: duodenum

characteristic: isolation source

text: Metaprevotella massiliensis

text: Metaprevotella massiliensis

characteristic: strain

text: Metaprevotella massiliensis genome


Burkholderia sp. RP11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cornell University

characteristic: INSDC center name

text: public

text: Burkholderia sp. RP11

tag: Namespace:Cornell University

characteristic: collection date

text: Burkholderia sp. RP11

text: isolate genome

characteristic: description

text: forest

characteristic: environment (biome)

text: forest

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Burkholderia sp. RP11

characteristic: organism

text: Stable isotope probing with hydroxybenoate in forest litter

characteristic: project name

text: Burkholderia sp. RP11

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Burkholderia sp. RP11


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: URMITE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: stool

characteristic: isolation source

text: Bacillus sp. Marseille-P3661

text: Bacillus sp. Marseille-P3661

characteristic: strain

text: Bacillus dionei genome

characteristic: title


text: URMITE

characteristic: INSDC center name

text: public

text: skin

text: Bacteroides sp. Marseille-P4118

text: Bacteroides sp. Marseille-P4118

text: Bacteroides cutis genome


text: Vibrio cholerae

characteristic: ArrayExpress-Species

text: V.cholerae O1_INET

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Vibrio cholerae

characteristic: common name

text: Vibrio cholerae

characteristic: organism

text: Vibrio cholerae

text: V.cholerae O1_INET


text: Vibrio Cholerae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Vibrio Cholerae

text: Vibrio cholerae

text: Vibrio cholerae

characteristic: subject id


text: Vibrio cholerae

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: Vibrio cholerae

text: Vibrio cholerae

text: Vibrio cholerae


E-MTAB-6238:Sample 1

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6238:Sample 1

tag: Namespace:Centre for Molecular Biology of Heidelberg University (ZMBH)

text: ArrayExpress

characteristic: broker name

text: control

characteristic: clinical history

text: human

text: Protocols: Whole blood ribo-minus and globin-clear

characteristic: description

text: normal

characteristic: disease

text: person 1

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-6238:Sample 2

text: Centre for Molecular Biology of Heidelberg University (ZMBH)

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-6238:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: control

text: human

characteristic: common name

text: normal

text: person 2

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-6238:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6238:Sample 3

text: ArrayExpress

text: control

text: human

text: normal

text: person 3

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 3


text: Vibrio Cholerae

characteristic: ArrayExpress-Species

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC first public

text: public

text: Vibrio Cholerae

text: Vibrio cholerae

characteristic: sample description

text: Vibrio cholerae


text: gut metagenome

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: gut metagenome

text: gut metagenome

text: gut metagenome


E-MTAB-6239:+RNAi HF 1

text: ZMBH - Heidelberg University

text: public

text: E-MTAB-6239:+RNAi HF 1

tag: Namespace:ZMBH - Heidelberg University

text: ArrayExpress

characteristic: broker name

text: Protocols: Culture in the presence or absence of Tetracyclin RNA extraction (Trifast) Standard Illumina kit

characteristic: description

text: bloodstream form

characteristic: developmental stage

text: polysome fraction

characteristic: fraction

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: tetracycline

characteristic: stimulus

text: +RNAi HF 1


E-MTAB-6239:+RNAi HF 2

text: ZMBH - Heidelberg University

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-6239:+RNAi HF 2

characteristic: Submitter Id

text: ArrayExpress

text: bloodstream form

text: polysome fraction

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: tetracycline

text: +RNAi HF 2


E-MTAB-6239:-RNAi HF 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ZMBH - Heidelberg University

text: public

text: E-MTAB-6239:-RNAi HF 1

text: ArrayExpress

text: bloodstream form

text: polysome fraction

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: -RNAi HF 1

characteristic: title


E-MTAB-6239:-RNAi HF 2

characteristic: ENA-LAST-UPDATE

text: ZMBH - Heidelberg University

text: public

text: E-MTAB-6239:-RNAi HF 2

text: ArrayExpress

text: bloodstream form

text: polysome fraction

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: Trypanosoma brucei

text: -RNAi HF 2


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: External Id

text: SC

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


E-MTAB-6240:RNA-IP 4EIP Elu 1

text: ZMBH - University of Heidelberg

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6240:RNA-IP 4EIP Elu 1

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP 4EIP Elu 1


E-MTAB-6240:RNA-IP 4EIP Elu 2

text: ZMBH - University of Heidelberg

characteristic: INSDC center name

text: public

text: E-MTAB-6240:RNA-IP 4EIP Elu 2

text: ArrayExpress

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP 4EIP Elu 2


E-MTAB-6240:RNA-IP 4EIP FT 1

text: ZMBH - University of Heidelberg

text: public

characteristic: SRA accession

text: E-MTAB-6240:RNA-IP 4EIP FT 1

text: ArrayExpress

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP 4EIP FT 1


E-MTAB-6240:RNA-IP 4EIP FT 2

text: ZMBH - University of Heidelberg

text: public

text: E-MTAB-6240:RNA-IP 4EIP FT 2

characteristic: Submitter Id

text: ArrayExpress

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: RNA-IP 4EIP FT 2


characteristic: ENA-CHECKLIST

text: GOETTINGEN GENOMICS LABORATORY

text: public

characteristic: collection date

text: Salt marsh

characteristic: environment (biome)

text: Archipelago

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Yes

characteristic: environmental sample

characteristic: further details

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

text: CCAGCASCYGCGGTAATTCC, see Stoeck et al. 2010 (Mol Ecol 19 Suppl 1:21-31); ACTTTCGTTCTTGATYRA, see Stoeck et al. 2010 (Mol Ecol 19 Suppl 1:21-31) - the primers contained extensions required for sequencing)

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

text: Eukaryotes in marsh soil


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Center for Hematology and Regenerative Medicine

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HT-CM

characteristic: title


characteristic: ENA-CHECKLIST

text: Center for Hematology and Regenerative Medicine

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens

text: HT-CM


text: UCSDMI

text: public

text: Treated soil

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Soil (non-saline)

text: soil

characteristic: env biome

text: agricultural soil

characteristic: env feature

text: soil

characteristic: env package

text: soil

text: Australia:WA:Yathroo

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: soil metagenome

text: UWA

text: TRUE

text: soil

characteristic: sample type

text: soil metagenome

text: Copolymers enhance selective bacterial community colonization for potential root zone applications


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Polyacrylic acid polymer

text: TRUE

text: Free-living

text: Non-saline

text: Soil (non-saline)

text: environmental material

text: anthropogenic environmental material

text: misc environment

text: organic material

text: Australia:WA:Yathroo

text: metagenome

text: UWA

text: TRUE

text: polymer additive

text: metagenome


characteristic: ENA-CHECKLIST

text: UCSDMI

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Polyacrylic acid polymer containing free mannan chains

text: TRUE

text: Free-living

text: Non-saline

text: Soil (non-saline)

text: environmental material

text: anthropogenic environmental material

text: misc environment

text: organic material

text: Australia:WA:Yathroo

text: metagenome

characteristic: organism

text: UWA

text: TRUE

text: polymer additive

text: metagenome

characteristic: title


text: UCSDMI

text: public

text: Dry soil

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Soil (non-saline)

text: soil

characteristic: env biome

text: agricultural soil

characteristic: env feature

text: soil

characteristic: env package

text: soil

text: Australia:WA:Yathroo

characteristic: geo loc name

text: soil metagenome

text: UWA

text: TRUE

text: soil

text: soil metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

text: Dry soil

text: TRUE

text: Free-living

text: Non-saline

text: Soil (non-saline)

text: soil

text: agricultural soil

text: soil

text: soil

text: Australia:WA:Yathroo

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: soil metagenome

text: UWA

text: TRUE

text: soil

characteristic: sample type

text: soil metagenome


characteristic: ENA-CHECKLIST

characteristic: External Id

text: ZMBH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: Protocols: RNA-IP protocol-Procyclic TAP-ZC3H30/SKO were grown at log phase till the density was 1.5million cells/ml and not more than 2million cells/ml. Cell culture was pulled at 1125xg for 10 minutes. It was washed with 10ml of MEM-Pros medium (without FCS), followed by UV crosslinking at (400mJ/square cm) while on ice, recentrifuged at 1125xg, for 10 minutes. The final cell pellet was washed with cold 1xPBS and proceeded for lysis and immunoprecipitation. T.brucei procyclic cells were grown at log phase till the density was 1.5million cells/ml and not more than 2million cells/ml. Cell pellet was washed with MEM-Pros (without FCS) and UV crosslinked 400mJ/square cm) while on ice. Log phase cell culture was heat shocked at 39 degrees for 1 hour before RNA extraction. Standard TRIFast FL protocol from PeqLab Standard Illumina library preparation protocol

text: procyclic form

characteristic: developmental stage

text: expression of ZC3H32 with N-terminal TAP tag in heterozygous ZC3H30 background

characteristic: genotype

text: not available

characteristic: individual

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

characteristic: strain


E-MTAB-6281:Procyclic ZC3H30 DKO10B

characteristic: ENA-FIRST-PUBLIC

text: ZMBH

text: public

characteristic: SRA accession

text: E-MTAB-6281:Procyclic ZC3H30 DKO10B

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Total RNA protocol from non-heat shocked cells. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. A total of 5million cells were harvested at 850xg, 8 minutes, washed with cold 1xPBS, and then proceeded to RNA extraction using TriFast from PeqLab. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. Grown at normal growth temperature of 27 degrees. Log phase cell culture was heat shocked at 39 degrees for 1 hour before RNA extraction. Stand chloroform RNA extraction using TriFast from PeqLab. Standrd Illumina library preparation protocol.

characteristic: description

text: procyclic form

text: homozygous ZC3H30 knockout

text: clone 10

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO10B

characteristic: title


E-MTAB-6281:Procyclic ZC3H30 DKO10BHS

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ZMBH

text: public

text: E-MTAB-6281:Procyclic ZC3H30 DKO10BHS

text: ArrayExpress

text: Protocols: Total RNA protocol from non-heat shocked cells. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. A total of 5million cells were harvested at 850xg, 8 minutes, washed with cold 1xPBS, and then proceeded to RNA extraction using TriFast from PeqLab. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. Stand chloroform RNA extraction using TriFast from PeqLab. Standrd Illumina library preparation protocol.

text: procyclic form

text: homozygous ZC3H30 knockout

text: clone 10

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO10BHS


E-MTAB-6281:Procyclic ZC3H30 DKO3

characteristic: External Id

text: ZMBH

characteristic: INSDC center name

text: public

text: E-MTAB-6281:Procyclic ZC3H30 DKO3

text: ArrayExpress

text: procyclic form

text: homozygous ZC3H30 knockout

text: clone 3

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO3


E-MTAB-6281:Procyclic ZC3H30 DKO3HS

text: ZMBH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6281:Procyclic ZC3H30 DKO3HS

text: ArrayExpress

text: procyclic form

text: homozygous ZC3H30 knockout

text: clone 3

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO3HS


text: ZMBH

text: public

characteristic: INSDC status

text: E-MTAB-6281:Procyclic ZC3H30 DKO-ectopic2

text: ArrayExpress

characteristic: broker name

text: procyclic form

text: ectopic expression of ZC3H30 in homozygous ZC3H30 knockout background

text: clone 2

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO-ectopic2


text: ZMBH

text: public

text: E-MTAB-6281:Procyclic ZC3H30 DKO-ectopic2HS

text: ArrayExpress

text: procyclic form

characteristic: developmental stage

characteristic: genotype

text: clone 2

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO-ectopic2HS


text: ZMBH

text: public

text: E-MTAB-6281:Procyclic ZC3H30 DKO-ectopic7

text: ArrayExpress

text: procyclic form

text: clone 7

characteristic: individual

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

characteristic: strain

text: Procyclic ZC3H30 DKO-ectopic7


characteristic: ENA-FIRST-PUBLIC

text: ZMBH

text: public

characteristic: SRA accession

text: E-MTAB-6281:Procyclic ZC3H30 DKO-ectopic7HS

text: ArrayExpress

text: procyclic form

text: clone 7

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Lister 427

text: Procyclic ZC3H30 DKO-ectopic7HS


text: Universite Lyon1

text: public

tag: Namespace:Universite Lyon1

characteristic: Submitter Id

text: A new species of Frankia infective on Alnus crispa

characteristic: description

text: Frankia sp. ARgP5

text: Frankia sp. ARgP5

text: Frankia canadensis a new species


Alouette

tag: attribute

text: James Hutton Institute

text: public

text: Alouette

text: potato

characteristic: common name

text: NBS-LRR enrichment sequencing from potato cultivars

text: Solanum tuberosum

characteristic: organism

text: Solanum tuberosum

characteristic: title


Bionica

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: James Hutton Institute

text: public

text: Bionica

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Cara

text: James Hutton Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Cara

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Craigs Snow White

text: James Hutton Institute

text: public

characteristic: INSDC status

text: Craigs Snow White

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Innovator RenSeq

text: James Hutton Institute

text: public

text: Innovator RenSeq

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Pentland Ace

text: James Hutton Institute

text: public

text: Pentland Ace

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Pentland Dell

text: James Hutton Institute

text: public

text: Pentland Dell

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Picasso

text: James Hutton Institute

text: public

text: Picasso

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


Toluca

text: James Hutton Institute

text: public

text: Toluca

text: potato

text: Solanum tuberosum

text: Solanum tuberosum


text: public

tag: Namespace:Iowa State University Department of Animal Science

unit: day

characteristic: age

text: Broiler

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: chicken

text: Protocols: In each biological replicate, birds were transferred at 17 days of age into pens within 4 temperature controlled chambers (2 for heat stress and 2 for thermoneutral). Both chicken lines (broiler and Fayoumi) were included in all 4 chambers. Birds were allowed to acclimate for 5 days at 25°C before the start of the treatment protocol. Warm/hot temperature regiment means birds were kept at 35 degrees celsius for 7 hours, otherwise birds were kept at 25 degrees celsius. Birds were euthanized by intravenous injection of sodium pentobarbital (Fort Dodge Animal Health, Fort Dodge, IA). Tissue samples from the bursa were collected into RNAlater (Ambion, Inc., Austin, TX), perfused for 24 hours at room temperature, and then stored at -80°C to preserve the RNA. Birds from both lines (broiler and Fayoumi) were assigned to four treatments: Thermoneutral + Saline, Thermoneutral + LPS, Heat + Saline, and Heat + LPS. At 22 days of age, birds in the Heat groups were exposed to 35°C for 7 hours. Birds in the Thermoneutral groups remained at 25°C. At the midpoint of heat stress (3.5 hours), birds in the LPS groups were given a subcutaneous injection of lipopolysaccharide (100 µg of LPS/kg of body weight based on average weight for each line at day 21). Birds in the Saline groups were injected with an equivalent volume of phosphate buffered saline. Total RNA was isolated from each bursal tissue sample using the RNAqueous Total RNA Isolation kit (Ambion, Inc., Austin, TX). Resulting RNA samples were DNase treated using the DNA-free DNA Removal kit (Ambion, Inc.). RNA quality was determined (all had RNA Integrity Score > 8.9) using the Eukaryote Total RNA Nano kit on the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Individual cDNA libraries were constructed from 0.5 µg of total RNA/sample using the Illumina TruSeq RNA Library Preparation kit version 2 (Illumina, Inc., San Diego, CA). Quality of cDNA libraries was confirmed using the DNA 1000 kit on the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Gallus gallus

text: Gallus gallus

text: bursa of Fabricius

characteristic: organism part

text: Gallus gallus

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

text: Iowa State University Department of Animal Science

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Broiler

text: ArrayExpress

text: chicken

characteristic: common name

characteristic: description

text: normal

text: wild type genotype

characteristic: individual

text: Gallus gallus

text: Gallus gallus

text: bursa of Fabricius

text: Gallus gallus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Fayoumi

text: ArrayExpress

text: chicken

text: normal

text: wild type genotype

text: Gallus gallus

characteristic: organism

text: Gallus gallus

text: bursa of Fabricius

text: Gallus gallus

text: female

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: Fayoumi

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: chicken

text: normal

text: wild type genotype

text: Gallus gallus

text: Gallus gallus

text: bursa of Fabricius

text: Gallus gallus

text: male


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: Broiler

text: ArrayExpress

text: chicken

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Gallus gallus

text: Gallus gallus

text: bursa of Fabricius

characteristic: organism part

text: Gallus gallus

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Universiti Malaysia Sarawak

text: public

tag: Namespace:Universiti Malaysia Sarawak

characteristic: Submitter Id

text: Malaria Research Centre, Universiti Malaysia Sarawak

characteristic: collecting institution

characteristic: collection date

text: Balbir Singh

characteristic: collector name

text: PCR

characteristic: diagnostic method

text: Malaysia

characteristic: geographic location (country and/or sea)

text: Kapit Division, Sarawak

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: male

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: whole blood

characteristic: isolation source host associated

text: malaria

characteristic: known pathogenicity

text: Plasmodium knowlesi

text: Plasmodium knowlesi

text: Plasmodium knowlesi infections from Kapit Division, Sarawak, Malaysia


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Universiti Malaysia Sarawak

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Balbir Singh

text: PCR

text: Malaysia

text: Kapit Division, Sarawak

text: human

text: recovered

text: female

text: whole blood

text: malaria

text: Plasmodium knowlesi

characteristic: organism

text: Plasmodium knowlesi

characteristic: title


ANG

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: UNIBE-IG

text: public

text: ANG

characteristic: Submitter Id

text: goat

characteristic: common name

text: Punjab/Pakistan

characteristic: geographic location (region and locality)

text: Capra hircus

text: Capra hircus

text: female

characteristic: sex

text: Pak-Angora from Pakistan

characteristic: sub species

text: blood

characteristic: tissue type

text: Pak-Angora goat


APZ

characteristic: ENA-FIRST-PUBLIC

text: UNIBE-IG

text: public

text: APZ

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Appenzeller goat from Switzerland

text: blood

text: Appenzeller goat


BAR

characteristic: External Id

text: UNIBE-IG

text: public

text: BAR

text: goat

text: Sargodha/Pakistan

text: Capra hircus

text: Capra hircus

text: female

text: Barbari from Pakistan

text: blood

text: Barbari goat


BEE

text: UNIBE-IG

characteristic: INSDC center name

text: public

text: BEE

text: goat

text: Punjab/Pakistan

text: Capra hircus

text: Capra hircus

text: female

text: Beetal from Pakistan

text: blood

text: Beetal goat


BEZ

text: UNIBE-IG

characteristic: INSDC first public

text: public

text: BEZ

text: wild goat

text: Greek and Switzerland

text: Capra aegagrus

text: Capra aegagrus

text: female and male

text: Bezoar goats from Greek

text: blood

text: Bezoar goat


BST

text: UNIBE-IG

characteristic: INSDC last update

text: public

text: BST

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Grisons Striped Goat from Switzerland

text: blood

text: Grisons Striped Goat


BUR

text: UNIBE-IG

text: public

characteristic: INSDC status

text: BUR

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Boer goat from Switzerland

text: blood

text: Boer goat


GFG

text: UNIBE-IG

text: public

characteristic: SRA accession

text: GFG

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Chamois colored goat from Switzerland

text: blood

text: Chamois colored goat


DDP

text: UNIBE-IG

text: public

text: DDP

text: goat

text: Multan/Punjab

text: Capra hircus

characteristic: organism

text: Capra hircus

text: female

text: Dera Din Panah from Pakistan

text: blood

text: Dera Din Panah goat


KAM

text: UNIBE-IG

text: public

text: KAM

text: goat

text: Sindh/Pakistan

text: Capra hircus

text: Capra hircus

text: female

text: Kamori from Pakistan

text: blood

text: Kamori goat

characteristic: title


NAC

characteristic: ENA-CHECKLIST

text: UNIBE-IG

text: public

text: NAC

text: goat

text: Punjab/Pakistan

text: Capra hircus

text: Capra hircus

text: female

text: Nachi from Pakistan

text: blood

text: Nachi goat


NER

characteristic: ENA-LAST-UPDATE

text: UNIBE-IG

text: public

text: NER

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Nera Verzasca goat from Switzerland

text: blood

text: Nera Verzasca


CAG

text: UNIBE-IG

text: public

text: CAG

characteristic: Submitter Id

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Capra Grigia from Switzerland

text: blood

text: Capra Grigia


SAN

text: UNIBE-IG

text: public

text: SAN

text: goat

characteristic: common name

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Saanen goat from Switzerland

text: blood

text: Saanen goat


STG

text: UNIBE-IG

text: public

text: STG

text: goat

text: Switzerland

characteristic: geographic location (region and locality)

text: Capra hircus

text: Capra hircus

text: female

text: St. Gallen Booted goat from Switzerland

text: blood

text: St. Gallen Booted goat


TED

text: UNIBE-IG

text: public

text: TED

text: goat

text: Punjab/Pakistan

text: Capra hircus

text: Capra hircus

text: female

characteristic: sex

text: Teddy from Pakistan

text: blood

text: Teddy goat


TOG

text: UNIBE-IG

text: public

text: TOG

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Toggenburger from Switzerland

characteristic: sub species

text: blood

text: Toggenburger goat


PAH

text: UNIBE-IG

text: public

text: PAH

text: goat

text: Khyber Pakhtunkhwa/Pakistan

text: Capra hircus

text: Capra hircus

text: female

text: Pahari from Pakistan

text: blood

characteristic: tissue type

text: Pahari goat


PFA

characteristic: ENA-FIRST-PUBLIC

text: UNIBE-IG

text: public

text: PFA

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Peacock goat from Switzerland

text: blood

text: Peacock goat


VAG

characteristic: External Id

text: UNIBE-IG

text: public

text: VAG

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: Valais Blacknecked goat from Switzerland

text: blood

text: Valais Blacknecked goat


ZWZ

text: UNIBE-IG

characteristic: INSDC center name

text: public

text: ZWZ

text: goat

text: Switzerland

text: Capra hircus

text: Capra hircus

text: female

text: African Dwarf goat form Africa

text: blood

text: African Dwarf goat


ChH Cheese

text: King Fahd Medical Research Centre

characteristic: INSDC first public

text: public

text: ChH Cheese

tag: Namespace:King Fahd Medical Research Centre

text: food fermentation metagenome

text: food fermentation metagenome

text: ChH Cheese


LBP

text: King Fahd Medical Research Centre

characteristic: INSDC last update

text: public

text: LBP

text: food fermentation metagenome

text: food fermentation metagenome

text: LBP


Mish

text: King Fahd Medical Research Centre

text: public

characteristic: INSDC status

text: Mish

text: food fermentation metagenome

text: food fermentation metagenome

text: Mish


PicH Pickle

text: King Fahd Medical Research Centre

text: public

characteristic: SRA accession

text: PicH Pickle

text: food fermentation metagenome

text: food fermentation metagenome

text: PicH Pickle


Yg-1 Yougurt

text: King Fahd Medical Research Centre

text: public

text: Yg-1 Yougurt

text: food fermentation metagenome

characteristic: organism

text: food fermentation metagenome

text: Yg-1 Yougurt


text: University Hospital Galway

text: public

tag: Namespace:University Hospital Galway

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Typhimurium

text: Typhimurium

characteristic: serovar

text: Subsp. I

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: University Hospital Galway

text: public

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Enteritidis

text: Enteritidis

text: Subsp. I

characteristic: sub species


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4,[5],12:i:-

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Infantis

text: Infantis

text: Subsp. I


text: University Hospital Galway

text: public

characteristic: SRA accession

characteristic: collection date

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhi

characteristic: organism

text: Typhi

text: Subsp. I


text: Anopheles gambiae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: African malaria mosquito

characteristic: common name

text: Anopheles gambiae

text: Anopheles gambiae

characteristic: subject id


text: Institute of Clinical Molecular Biology

text: public

tag: Namespace:Institute of Clinical Molecular Biology

text: metagenome

text: metagenome

characteristic: title


text: Plasmodium falciparum

characteristic: ENA-CHECKLIST

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Microbial Ecology of Health UNIT, University of Bologna

text: Faecal Microbiota

characteristic: description

text: organic feature

characteristic: environment (biome)

text: anthropogenic environmental material

characteristic: environment (feature)

text: human-gut

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: metagenomes

text: Early signature of aGvHD development in the gut microbiota composition of pediatric patients given allogeneic hematopoietic cell transplantation for hematological disorders

characteristic: project name

text: metagenomes

text: Illumina

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Microbial Ecology of Health UNIT, University of Bologna

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Faecal Microbiota

text: organic feature

text: anthropogenic environmental material

text: human-gut

text: Italy

text: human-gut

text: metagenome

text: metagenomes

characteristic: organism

text: metagenomes

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

tag: Namespace:NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: Time 0 sample from individual AR00041

characteristic: description

text: Ethiopia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Oropharyngeal swab

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: serovar

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Time 8 sample from individual AR00041

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Time 0 sample from individual AR00087

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

characteristic: collection date

text: Time 9 sample from individual AR00087

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 0 sample from individual AR00093

characteristic: description

text: Ethiopia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 8 sample from individual AR00093

text: Ethiopia

text: healthy

text: Homo sapiens

characteristic: isolate

text: Oropharyngeal swab

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: serovar

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Time 0 sample from individual AR00145

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Time 7 sample from individual AR00145

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

characteristic: collection date

text: Time 0 sample from individual AR00226

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 9 sample from individual AR00226

characteristic: description

text: Ethiopia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 0 sample from individual AR00356

text: Ethiopia

text: healthy

text: Homo sapiens

characteristic: isolate

text: Oropharyngeal swab

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: serovar

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Time 7 sample from individual AR00356

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Time 0 sample from individual AR00639

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

characteristic: collection date

text: Time 9 sample from individual AR00639

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 0 sample from individual AR01252

characteristic: description

text: Ethiopia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 9 sample from individual AR01252

text: Ethiopia

text: healthy

text: Homo sapiens

characteristic: isolate

text: Oropharyngeal swab

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: serovar

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Time 0 sample from individual AR02128

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Time 9 sample from individual AR02128

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

characteristic: collection date

text: Time 0 sample from individual AR02148

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

characteristic: organism

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 7 sample from individual AR02148

characteristic: description

text: Ethiopia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis


text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

text: public

text: Time 0 sample from individual AR02217

text: Ethiopia

text: healthy

text: Homo sapiens

characteristic: isolate

text: Oropharyngeal swab

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: serovar

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: NORWEGIAN INSTITUTE OF PUBLIC HEALTH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Time 8 sample from individual AR02217

text: Ethiopia

text: healthy

text: Homo sapiens

text: Oropharyngeal swab

text: Neisseria meningitidis

text: Neisseria meningitidis