characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000372) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000346,EMOSE_N010000348

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 225 minutes, starting at 11:25 and ending at 15:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000372

characteristic: title


status: PUBLIC

tag: attribute

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

unit: DD

unit: practical salinity unit

tag: Namespace:PANGAEA Data Publisher for Earth and Environmental Science

unit: m

text: This sample (EMOSE_N010000405) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000346,EMOSE_N010000348

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 152 minutes, starting at 13:58 and ending at 16:30 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

unit: ºC

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000405


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000411) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000346,EMOSE_N010000348

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 167 minutes, starting at 17:03 and ending at 19:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000411

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000373) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000412,EMOSE_N010000351

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 225 minutes, starting at 11:25 and ending at 15:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000373


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000406) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000373,EMOSE_N010000412,EMOSE_N010000351

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 152 minutes, starting at 13:58 and ending at 16:30 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000406

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000412) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000351

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 60 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 167 minutes, starting at 17:03 and ending at 19:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000412


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000374) was created in silico using samples EMOSE_N010000374, EMOSE_N010000375, EMOSE_N010000376, EMOSE_N010000377 and EMOSE_N010000378. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (200 ng each) were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 496 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 445 minutes, starting at 17:20 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N020000374

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000374) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 98 minutes, starting at 17:20 and ending at 18:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000374


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000375) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 110 minutes, starting at 19:19 and ending at 21:09 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000375

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000376) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 285 minutes, starting at 20:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000376


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000377) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000378,EMOSE_N010000385

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 132 minutes, starting at 20:40 and ending at 22:52 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000377

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000378) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000377,EMOSE_N010000376,EMOSE_N010000375,EMOSE_N010000384,EMOSE_N010000374,EMOSE_N010000386,EMOSE_N010000383,EMOSE_N010000385

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 96 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 165 minutes, starting at 22:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000378


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000340) was created in silico using samples EMOSE_N010000340, EMOSE_N010000344, EMOSE_N010000346, EMOSE_N010000348 and EMOSE_N010000350. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (200 ng each) were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 496 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 445 minutes, starting at 17:20 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N020000340

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000340) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000344,EMOSE_N010000346,EMOSE_N010000348

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 140 minutes, starting at 17:20 and ending at 19:40 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000340


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000344) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000346,EMOSE_N010000348

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 132 minutes, starting at 19:19 and ending at 21:31 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000344

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000346) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000348

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 285 minutes, starting at 20:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000346


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000348) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000350,EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000346

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 132 minutes, starting at 20:40 and ending at 22:52 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000348

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000350) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000411,EMOSE_N010000372,EMOSE_N010000405,EMOSE_N010000340,EMOSE_N010000344,EMOSE_N010000346,EMOSE_N010000348

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 96 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 165 minutes, starting at 22:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000350


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000342) was created in silico using samples EMOSE_N030000342, EMOSE_N010000345, EMOSE_N010000347, EMOSE_N010000349 and EMOSE_N010000351. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate (200 ng each) were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 496 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 445 minutes, starting at 17:20 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N020000342

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N030000342) was created in silico using samples EMOSE_N010000342 and EMOSE_N010000680. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. Filters from the pooled samples were combined before extraction. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000412,EMOSE_N010000351

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 98 minutes, starting at 17:20 and ending at 18:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N030000342


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000342) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000680

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 50 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 98 minutes, starting at 17:20 and ending at 18:58 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000342

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000680) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000342

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000680


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000345) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000412,EMOSE_N010000351

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 110 minutes, starting at 19:19 and ending at 21:09 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000345

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000347) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000412,EMOSE_N010000351

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 285 minutes, starting at 20:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000347


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000349) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000412,EMOSE_N010000351

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 132 minutes, starting at 20:40 and ending at 22:52 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000349

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000351) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705300746Z_DAY1_EVENT-PUMP) occurred at position latitudeN=42.486817 and longitudeE=003.168633, on date/time=2017-05-30T07:46Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000349,EMOSE_N010000347,EMOSE_N010000345,EMOSE_N010000342,EMOSE_N010000680,EMOSE_N010000406,EMOSE_N010000373,EMOSE_N010000412

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 96 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 165 minutes, starting at 22:00 and ending at 00:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705300746Z_DAY1_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N010000351


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000704) was created in silico using samples EMOSE_N010000704, EMOSE_N010000705, EMOSE_N010000706, EMOSE_N010000707, EMOSE_N010000708, EMOSE_N010000709, EMOSE_N010000710, EMOSE_N010000717, EMOSE_N010000712 and EMOSE_N010000713. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 1000 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 91 minutes, starting at 10:24 and ending at 11:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N020000704

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000704) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 91 minutes, starting at 10:24 and ending at 11:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000704


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000705) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 130 minutes, starting at 10:32 and ending at 12:42 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000705

characteristic: title