characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-CGAGGCTG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

unit: µmol/kg

text: aquatic

text: freshwater

text: small lake

text: Sweden

unit: m

unit: DD

text: mimarks-survey

text: stratified

text: epilimnion

text: CTCTCTAT-AAGAGGCA

characteristic: multiplex identifiers

text: freshwater metagenome

text: initial denaturation step at 95 �C for 30 s, followed by 20 cycles of dissociation at 95 �C for 10 s, annealing at 62 �C for 30 s extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C. then second round: initial denaturation step at 95 �C for 30 s, followed by 15 cycles of dissociation at 95 �C for 10 s, annealing at 66 �C for 30 s, extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

text: Lake Erken SSU rRNA gene amplicon timeseries

characteristic: project name

unit: °C

characteristic: sample storage temperature

unit: g

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

unit: ºC

characteristic: temperature

characteristic: total depth of water column

unit: µg/L

characteristic: total nitrogen

unit: µmol/L

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-GTAGAGGA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: CTCTCTAT-CTTATACC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-TCTAGCTG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: CTCTCTAT-TGCTATGG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-GCTATACC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: CTCTCTAT-GCATAACC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-CTTGCCTA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: CTCTCTAT-ATGGACAA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: CTCTCTAT-TGCCTAGA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-TAAGGCGA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: TATCCTCT-CGTACTAG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-AGGCAGAA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: TATCCTCT-TCCTGAGC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-GGACTCCT

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: TATCCTCT-CTCTCTAC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-CAGAGAGG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: TATCCTCT-CGAGGCTG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-AAGAGGCA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: TATCCTCT-CTTATACC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: TATCCTCT-TCTAGCTG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: TATCCTCT-TGCTATGG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-GCTATACC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: TATCCTCT-GCATAACC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-CTTGCCTA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: TATCCTCT-ATGGACAA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: TATCCTCT-TGCCTAGA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-TAAGGCGA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-CGTACTAG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-AGGCAGAA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-TCCTGAGC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-GGACTCCT

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-TAGGCATG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-CTCTCTAC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-CAGAGAGG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-GCTACGCT

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-CGAGGCTG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-AAGAGGCA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: AGAGTAGA-GTAGAGGA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: epilimnion

characteristic: layer sampled

text: AGAGTAGA-CTTATACC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-TCTAGCTG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-TGCTATGG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-GCTATACC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-GCATAACC

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: AGAGTAGA-CTTGCCTA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: metalimnion

characteristic: layer sampled

text: AGAGTAGA-ATGGACAA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: ice cover w circulation

text: mixed

text: TAGATCGC-CAGAGAGG

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AGAGTAGA-TGCCTAGA

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: GTAAGGAG-TAAGGCGA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: metalimnion

characteristic: layer sampled

text: GTAAGGAG-CGTACTAG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: epilimnion

text: GTAAGGAG-TCCTGAGC

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: description

characteristic: dissolved oxygen

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: GTAAGGAG-GGACTCCT

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: ice cover w circulation

text: mixed

text: TAGATCGC-GCTACGCT

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

characteristic: secchi depth visibility (m)

text: Illumina Miseq PE

characteristic: sequencing method

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


text: Bladder Bacterium

characteristic: ArrayExpress-PHENOTYPE

text: Escherichia coli

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Escherichia coli

characteristic: common name

text: Escherichia coli

characteristic: organism

text: Bladder Bacterium

characteristic: phenotype

text: Escherichia coli

characteristic: subject id

characteristic: title


text: Bladder Bacterium

characteristic: ArrayExpress-PHENOTYPE

text: Aerococcus urinae

text: SC

text: public

text: Aerococcus urinae

text: Aerococcus urinae

text: Bladder Bacterium

text: Aerococcus urinae


text: Bladder Bacterium

text: Bifidobacterium longum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Bifidobacterium longum

text: Bifidobacterium longum

text: Bladder Bacterium

text: Bifidobacterium longum


E-MTAB-5983:Sample 1

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5983:Sample 1

tag: Namespace:Toxicogenomics Department Maastricht Universtiy

text: ArrayExpress

characteristic: broker name

text: mixed pool of Hu8119, Hu1591 and Hu1540 cell lines

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: Protocols: Cryopreserved Primary Human Hepatocytes (PHH) of Invitrogen were bought of 3 individuals (Hu8119, Hu1591 and Hu1540). Cause of death for Hu8119 was CVA/Stroke, while this was unknown for Hu1591 and Hu1540. Cryopreserved Primary Human Hepatocytes (PHH, Invitrogen) of 3 individuals (Hu8119, Hu1591 and Hu1540) were thawed for 1 minute at 37°C in a water bath. Next, these PHH were pooled in order to bypass inter-individual variability in susceptibility to toxicants and cultured in 6-well plates in a collagen sandwich, according to the supplier’s protocol (Invitrogen). In brief, 3 vials (one per individual) were thawed in 50mL CHRM medium (CHRM CM7000, Invitrogen) and centrifuged for 10 minutes at 100g at 4ºC. After removing the supernatant, the cell pellet was dissolved in CHPM medium (CHPM CM9000, Invitrogen), which contain FBS, at a concentration of 1.0*10^6 cells/mL. Pooled PHH were cultured in collagen-precoated 24-well plates (Gibco) (density: 0.35*10^6 cells/well) and cells were attached to the wells in the incubator for 4 hours at 37ºC. After the incubation, the debris was removed by shaking and washing the cells twice with William’s medium E (WME CM6000, Invitrogen). Subsequently, cells were covered by 150 µL/well of 1.0mg/mL collagen-mixture (containing 10*DMEM, 1.0mg/mL Collagen type 1 (BD), 0.2M NaOH and, MQ) and incubated at 37°C for approximately 30 minutes until the collagen was fixed. After that, culture medium (WME + 20mL Hepatocyte Supplement Pack (CM4000, Invitrogen) substituted with 1% penicillin/streptomycin (Gibco)) was added. Primary human hepatocytes (PHH) were exposed daily for 3 days to 15 mM VPA after which DNA was isolated. After terminating the VPA treatment, part of the cells were kept with medium for 3 days (washout-period (WO-period)) in order to investigate the eventual persistence of VPA-induced methylome changes. We have used the Trizol LS Reagent protocol (Life Technologies) Libraries were prepared using 300 ng of fragmented DNA (~200 bp) and the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), according to the manufacturer’s protocol. The purified adaptor-ligated DNAs were used for Methylated DNA Immuno-Precipitation (MeDIP), according to the manufacturer’s instructions of the MagMeDIP kit (Diagenode) and IPure kit (Diagenode). Starting from the Methylated DNA samples, methylated DNAs were size selected and ligated for sequencing using the TruSeq® PE Cluster Kit V3, according to the manufacturer’s instructions (Illumina)

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: Sample 1


E-MTAB-5983:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Toxicogenomics Department Maastricht Universtiy

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5983:Sample 10

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 10


E-MTAB-5983:Sample 11

text: public

characteristic: SRA accession

text: E-MTAB-5983:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 11

characteristic: title


E-MTAB-5983:Sample 12

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5983:Sample 12

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 12


E-MTAB-5983:Sample 13

text: public

text: E-MTAB-5983:Sample 13

text: ArrayExpress

text: hepatocyte

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 13


E-MTAB-5983:Sample 14

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5983:Sample 14

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 14


E-MTAB-5983:Sample 15

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5983:Sample 15

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 15


E-MTAB-5983:Sample 16

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5983:Sample 16

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 16


E-MTAB-5983:Sample 2

text: public

text: E-MTAB-5983:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 2


E-MTAB-5983:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5983:Sample 3

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-5983:Sample 4

text: public

text: E-MTAB-5983:Sample 4

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 4


E-MTAB-5983:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5983:Sample 5

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 5


E-MTAB-5983:Sample 6

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-5983:Sample 6

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 6


E-MTAB-5983:Sample 7

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5983:Sample 7

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 7


E-MTAB-5983:Sample 8

text: public

characteristic: SRA accession

text: E-MTAB-5983:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 8


E-MTAB-5983:Sample 9

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5983:Sample 9

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 9


text: Glasgow Polyomics

text: public

tag: Namespace:Glasgow Polyomics

text: RNA-seq of host/pathogen interaction during infection

text: Citrobacter rodentium

text: Citrobacter rodentium

characteristic: title


E-MTAB-5984:Sample 1

text: public

text: E-MTAB-5984:Sample 1

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: Protocols: Cryopreserved Primary Human Hepatocytes (PHH, Invitrogen) of 3 individuals (Hu8119, Hu1591 and Hu1540) were thawed for 1 minute at 37°C in a water bath. Next, these PHH were pooled in order to bypass inter-individual variability in susceptibility to toxicants and cultured in 6-well plates in a collagen sandwich, according to the supplier’s protocol (Invitrogen). In brief, 3 vials (one per individual) were thawed in 50mL CHRM medium (CHRM CM7000, Invitrogen) and centrifuged for 10 minutes at 100g at 4ºC. After removing the supernatant, the cell pellet was dissolved in CHPM medium (CHPM CM9000, Invitrogen), which contain FBS, at a concentration of 1.0*10^6 cells/mL. Pooled PHH were cultured in collagen-precoated 24-well plates (Gibco) (density: 0.35*10^6 cells/well) and cells were attached to the wells in the incubator for 4 hours at 37ºC. After the incubation, the debris was removed by shaking and washing the cells twice with William’s medium E (WME CM6000, Invitrogen). Subsequently, cells were covered by 150 µL/well of 1.0mg/mL collagen-mixture (containing 10*DMEM, 1.0mg/mL Collagen type 1 (BD), 0.2M NaOH and, MQ) and incubated at 37°C for approximately 30 minutes until the collagen was fixed. After that, culture medium (WME + 20mL Hepatocyte Supplement Pack (CM4000, Invitrogen) substituted with 1% penicillin/streptomycin (Gibco)) was added. Primary human hepatocytes (PHH) were exposed daily for 3 days to 15 mM VPA after which RNA was isolated. After terminating the VPA treatment, part of the cells were kept with medium for 3 days (washout-period (WO-period)) in order to investigate the eventual persistence of VPA-induced transcriptome changes. Starting from the RNA samples, RNAs were size selected and ligated for sequencing following the TruSeq™ SBS Kit v3 paired-end by sequencing by synthesis (SBS) We have used the Trizol LS Reagent protocol (Life Technologies)

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 1


E-MTAB-5984:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5984:Sample 2

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 2


E-MTAB-5984:Sample 3

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-5984:Sample 3

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 3


E-MTAB-5984:Sample 4

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5984:Sample 4

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 4


E-MTAB-5984:Sample 5

text: public

characteristic: SRA accession

text: E-MTAB-5984:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 5


E-MTAB-5984:Sample 6

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5984:Sample 6

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 6


E-MTAB-5984:Sample 7

text: public

text: E-MTAB-5984:Sample 7

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 7

characteristic: title


E-MTAB-5984:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5984:Sample 8

text: ArrayExpress

text: hepatocyte

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 8


E-MTAB-5984:Sample 9

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-5984:Sample 9

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 9


E-MTAB-5984:Sample 10

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5984:Sample 10

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 10


E-MTAB-5984:Sample 11

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5984:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 11


E-MTAB-5984:Sample 12

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5984:Sample 12

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 12


E-MTAB-5984:Sample 13

text: public

text: E-MTAB-5984:Sample 13

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 13


E-MTAB-5984:Sample 14

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5984:Sample 14

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 14

characteristic: title


E-MTAB-5984:Sample 15

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-5984:Sample 15

text: ArrayExpress

text: hepatocyte

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 15


E-MTAB-5984:Sample 16

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5984:Sample 16

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 16


E-MTAB-5984:Sample 17

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5984:Sample 17

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 17


E-MTAB-5984:Sample 18

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5984:Sample 18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 18


E-MTAB-5984:Sample 19

text: public

text: E-MTAB-5984:Sample 19

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 19


E-MTAB-5984:Sample 20

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-5984:Sample 20

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 20


E-MTAB-5984:Sample 21

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-5984:Sample 21

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 21

characteristic: title


E-MTAB-5984:Sample 22

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5984:Sample 22

text: ArrayExpress

text: hepatocyte

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: Sample 22


E-MTAB-5984:Sample 23

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5984:Sample 23

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: Sample 23


E-MTAB-5984:Sample 24

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5984:Sample 24

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: replicate

text: Homo sapiens

text: Sample 24


text: UIUC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Kellis 2

type: has member


text: ISTANBUL UNIVERSITY

text: public

tag: Namespace:ISTANBUL UNIVERSITY

text: metagenome

text: metagenome


text: male

characteristic: ArrayExpress-SEX

text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: human

text: Male

characteristic: gender

text: Homo sapiens

text: Homo sapiens

characteristic: subject id


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Centre for Genome Enabled Biology and Medicine, University of Aberdeen

text: ArrayExpress

characteristic: broker name

text: Protocols: JC50 background strain Cells were incubated overnight in 5 ml volumes in the indicated conditions and transferred to fresh medium in 50 mL culture flasks for 4 hours (30C, 200 rpm) to an OD600 = 0.6. Half of the samples were treated with 2.5 mM DPTA NONOate. The other half of the samples remained no stress controls. RNA was obtained via Qiazol/chloroform extraction (QIAgen) according to the manufacturer’s instructions and assessed for quality by nanodrop. RNA was DNAse treated (TURBO DNAse, Ambion) according to the manufacturer’s instructions and assessed for quality by Agilent Bioanalyser (RIN>8). The sequencing library was prepared with the Ion Total RNA-Seq Kit v2 from Thermo Fisher Scientific (Life Tech) using ribosomal RNA depleted total RNA.

text: Hog1 deletion

characteristic: genotype

text: YPD

characteristic: growth condition

text: Candida albicans

characteristic: organism

text: Candida albicans

text: Sequencing Run 1

text: Candida albicans

characteristic: strain


characteristic: External Id

text: Centre for Genome Enabled Biology and Medicine, University of Aberdeen

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: description

text: Hog1 deletion

text: YPD

text: Candida albicans

text: Candida albicans

text: Sequencing Run 1

text: Candida albicans

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: YPD

text: Candida albicans

text: Candida albicans

text: Sequencing Run 1

characteristic: replicate

text: Candida albicans


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: Hog1 deletion

text: YPD

text: Candida albicans

text: Candida albicans

text: Sequencing Run 2

text: Candida albicans


text: public

text: ArrayExpress

characteristic: broker name

text: wild type genotype

characteristic: genotype

text: YPD

text: Candida albicans

text: Candida albicans

text: Sequencing Run 2

text: Candida albicans


text: WT

characteristic: ArrayExpress-PHENOTYPE

text: male

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: house mouse

characteristic: common name

text: Male

characteristic: gender

text: Mus musculus

characteristic: organism

text: WT

characteristic: phenotype

text: Mus musculus

characteristic: subject id


text: WT

text: male

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Male

text: Mus musculus

text: WT

text: Mus musculus

text: C12 WT

characteristic: title


text: WT

text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Male

text: Mus musculus

text: WT

text: Mus musculus

text: C12 KO


text: WT

characteristic: ArrayExpress-PHENOTYPE

text: male

characteristic: ArrayExpress-SEX

text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: house mouse

text: Male

text: Mus musculus

text: WT

text: Mus musculus

text: C13 WT


text: WT

text: male

text: Mus musculus

text: SC

text: public

text: house mouse

characteristic: common name

text: Male

characteristic: gender

text: Mus musculus

characteristic: organism

text: WT

text: Mus musculus

text: C13 KO


text: WT

text: male

text: Mus musculus

characteristic: External Id

text: SC

text: public

text: house mouse

text: Male

text: Mus musculus

text: WT

characteristic: phenotype

text: Mus musculus

characteristic: subject id

text: FLAG CON


text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

unit: mEq/L

characteristic: alkalinity

characteristic: ammonium

characteristic: collection date

text: freshwater metagenome

unit: mS/cm

characteristic: conductivity

characteristic: description

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: stratified

characteristic: lake stratification

text: hypolimnion

characteristic: layer sampled

text: AATCGGCA-TCGCCTTA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina Miseq PE

characteristic: sequencing method

characteristic: silicate

unit: mg/L

characteristic: suspended particulate matter

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: alkalinity

characteristic: ammonium

characteristic: collection date

text: freshwater metagenome

characteristic: common name

characteristic: conductivity

characteristic: description

text: aquatic

characteristic: environment (biome)

text: freshwater

characteristic: environment (feature)

text: small lake

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: circulation

characteristic: lake stratification

text: mixed

characteristic: layer sampled

text: AATCGGCA-CAGCCTCG

text: freshwater metagenome

characteristic: organism

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

text: freshwater metagenome

text: Illumina Miseq PE

text: SSU rRNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: alkalinity

characteristic: ammonium

characteristic: collection date

text: freshwater metagenome

text: aquatic

text: freshwater

text: small lake

text: Sweden

text: mimarks-survey

text: stratified

text: hypolimnion

text: TAGGACCT-TCTAGGCA

characteristic: multiplex identifiers

text: freshwater metagenome

characteristic: pcr conditions

text: CCTACGGGNGGCWGCAG-GACTACHVGGGTATCTAATCC

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: freshwater metagenome

text: Illumina Miseq PE

characteristic: sequencing method

characteristic: silicate

characteristic: suspended particulate matter

text: SSU rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column

characteristic: total nitrogen

characteristic: total phosphorus

text: water

characteristic: water environmental package