E-MTAB-6446:MG1655 PcseI 1

characteristic: ENA-CHECKLIST

text: The Wadsworth Center, NYSDOH

text: public

text: E-MTAB-6446:MG1655 PcseI 1

tag: Namespace:The Wadsworth Center, NYSDOH

text: ArrayExpress

characteristic: broker name

text: Protocols: 2mL overnights were grown in LB 37C at 225rpm and then subcultured 1:100. At an OD 0.5-0.7, RNA was harvested using the hot phenol method. Hot phenol RNA prep: transfer 1mL of subculture to microcentrifuge tube. Spin 1mL at max speed for 1 min and remove supernatant. Resuspend in 0.4mL ethanol+5% acid phenol/chloroform, spin 1 min at max speed and remove supernatant. Resuspend in 1mL 1x TBS, spin 1 min at max speed and remove supernatant. Resuspend in 0.5mL RNA Lysis Buffer (2% SDS, 4mM EDTA) and boil samples for 3 min. Add 0.5mL acid phenol/chloroform to each tube and incubate for 6 min at 65 °C. Incubate 5 min on ice then spin tubes at max speed for 6 min. Transfer upper phase to phase lock tube, add equal volume (0.4mL) chloroform:isoamyl alcohol 24:1 and spin 6 min at max speed. Transfer upper phase to new phase lock tube, add equal volume (0.4mL) chloroform:isoamyl alcohol 24:1 and spin 6 min at max speed. Transfer upper phase to new tube containing 40uL 3M Na Acetate+2uL glycogen (20mg/mL stock). Add 1mL ice cold EtOH and precipitate at -20 °C overnight or 15min at -80 °C. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 15uL nuclease free water. DNase treat RNA - set up 50uL reaction including 8-10ug RNA, 5uL of 10X buffer and 1uL DNase enzyme (Ambion). Incubate 30 min at 37 °C. Stop reaction by adding 352uL DEPC H2O + 134uL acid phenol:chloroform + 268uL chloroform:isoamyl alcohol 24:1. Transfer all to phase lock tube and spin at max speed for 6 min. Transfer upper phase to new microcentrifuge tube containing 40uL 3M Na Acetate + 2uL glycogen (20mg/mL stock). Add 1mL ice cold EtOH and precipitate at -20 °C overnight or 15min at -80 °C. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 15uL nuclease free water. Remove ribosomal RNA using the RiboZero kit from EpiCentre - wash magnetic beads 2x in RNase free water, then resuspend in Resuspension Solution. Add 32.5uL beads to each tube then add 0.5uL RNase inhibitor. Mix together in final volume of 20uL: 2.5ug DNase treated RNA + 2uL Ribozero reaction buffer + 4uL Ribozero rRNA removal solution. Incubate at 68 °C for 10 min followed by RT for 5 min. Add RNA to beads, mix by pipetting and vortexing for 10 s. Incubate at RT for 5 min. Vortex for 5 s then incubate at 50 °C for 5 min. Place tubes on magnetic stand for 1 min and transfer RNA to new tube. Bring volume to 180uL with DEPC H2O, then add 18uL 3M Na Acetate + 2uL glycogen (20mg/mL stock). Add 0.6mL ice cold EtOH and precipitate at -20 °C overnight. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 8uL nuclease free water. Prepare libraries using EpiCentre ScriptSeq v2 kit following kit protocol. Fragment RNA and anneal cDNA synthesis primer in 12uL reaction containing nuclease free water + 40ng rRNA depleted RNA + 1uL RNA Fragmentation solution + 2uL cDNA Synthesis primer. Incubate at 85 °C for 5 min then place tubes on ice. Synthesize DNA by adding 3uL cDNA Synthesis Premix + 0.5uL 100mM DTT + 0.5uL StarScript Reverse Transcriptase. Incubate at 25 °C for 5 min then 42 °C for 20 min. Cool tubes to 37 °C. Add 1uL Finishing solution and incubate at 37 °C for 10 min then 95 °C for 3 min. Cool tubes to 25 °C then add 7.5uL Terminal Tagging Premix + 0.5uL DNA Polymerase to terminally tag the cDNA. Incubate at 25 °C for 15 min, 95 °C for 3 min, then cool to 4 °C. Purify the cDNA using the Qiagen MinElute PCR Purification Kit - Add 5V Buffer PB to sample and transfer all to MinElute column. Spin 1 min at max speed. Discard flow through. Add 0.75mL Buffer PE to column and spin 1 min at max speed. Discard flow through. Spin column for 1 min at max speed then transfer column to new tube. Add 23.4uL Buffer EB to column, incubate 1 min at room temp, then spin 1 min at max speed. Amplify library by setting up 50uL PCR using 22.5uL purified DNA. Add 25uL FailSafe PCR Premix E + 1uL Forward PCR primer + 1uL ScriptSeq Index PCR Primer + 0.5uL FailSafe PCR Enzyme (1.25U). Heat at 95 °C for 1 min, then 12 cycles of 95 °C for 30 sec, 55 °C for 30 sec and 68 °C for 3 min. Incubate at 68 °C for 7 minutes. Purify sample using AMPure XP Purification protocol. Add 50uL beads to each pcr sample. Mix by pipetting 10x. Transfer beads+sample to new tube and incubate at room temp for 15 min. Put tubes on magnetic stand for 5 min then remove supernatant. Wash beads 2x, each time with 0.2mL of 80% EtOH, let stand 1 min then remove supernatant. Air dry beads at room temp for 15 min. Add 12uL nuclease free water, remove tubes from magnetic stand and resuspend beads in water. Incubate at room temp for 2 min. Place tubes on magnetic stand for 5 min. Library is in supernatant. Transfer to new tube. Quantify using Qubit.

characteristic: description

text: cas3 PcseI

characteristic: genotype

text: Escherichia coli str. K-12 substr. MG1655

text: MG1655 PcseI 1


E-MTAB-6446:MG1655 PcseI 2

status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Wadsworth Center, NYSDOH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6446:MG1655 PcseI 2

characteristic: Submitter Id

text: ArrayExpress

text: cas3 PcseI

characteristic: organism

text: MG1655 PcseI 2

characteristic: title


E-MTAB-6446:MG1655 PcseI CRISPRI 1

text: The Wadsworth Center, NYSDOH

text: public

text: E-MTAB-6446:MG1655 PcseI CRISPRI 1

text: ArrayExpress

text: cas3 PcseI CRISPRI

text: MG1655 PcseI CRISPRI 1


E-MTAB-6446:MG1655 PcseI CRISPRI 2

text: The Wadsworth Center, NYSDOH

text: public

text: E-MTAB-6446:MG1655 PcseI CRISPRI 2

text: ArrayExpress

text: cas3 PcseI CRISPRI

text: MG1655 PcseI CRISPRI 2


text: synthetic construct

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: synthetic construct

characteristic: common name

text: synthetic construct

text: synthetic construct

characteristic: subject id


E-MTAB-6432:Sample 2

characteristic: ENA-CHECKLIST

text: BAH-GU

text: public

text: E-MTAB-6432:Sample 2

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Lung tissue was formalin fixed and paraffin embedded. Adjacent sections were stained for phospho-ERK expression. Upon orientation of tumours, p-ERK high and p-ERK low tumour regions were captured by Laser Capture Microdissection. Multiple isolates from multiple tumours were collected from each of 4 mice and pooled by p-ERK status (ie. 2 pools per mouse). P-ERK levels were determined by IHC and were binary as either very weak or very strong signal. Mice were euthanised humanely by CO2 inhalation followed by cervical dislocation. The chest cavity was opened surgically and mice were cardiac perfused with PBS, followed by formalin. Lungs and trachea were removed intact and gently inflated with formalin. Tissue was fixed overnight prior to dehydration and embedding in paraffin All mice were induced at 8 weeks of age and harvested 6 weeks after induction For tumour induction, mice aged 8 weeks were anaesthetised and inoculated with 10^7 Adeno-CMV-CRE particles by intra-nasal inhalation as described in Murphy, Junttila et al., Cancer Cell 2008. Tumours were allowed to develop over the ensuing 6 weeks without further intervention. Total tissue RNA was isolated using the RNEasy FFPE kit according to manufacturer’s directions and ribosomal RNA was depleted with Ribo-Zero (Epicentre). Synthesis of cDNA was performed using the SMARTER Stranded random primed RNA-SEQ kit (Takara/Clontech), resulting in cDNA libraries flanked by Illumina indexing primers.

text: adenocarcinoma

characteristic: disease

text: Mus musculus

text: Mus musculus

text: lung epithelium

characteristic: organism part

text: p-ERK High

characteristic: phenotype

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: FVBN x C57BL/6

characteristic: strain

text: Sample 2


E-MTAB-6432:Sample 4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BAH-GU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6432:Sample 4

text: ArrayExpress

text: house mouse

characteristic: description

text: adenocarcinoma

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lung epithelium

text: p-ERK High

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 4


E-MTAB-6432:Sample 6

text: BAH-GU

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6432:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung epithelium

text: p-ERK High

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 6

characteristic: title


E-MTAB-6432:Sample 8

characteristic: ENA-CHECKLIST

text: BAH-GU

text: public

text: E-MTAB-6432:Sample 8

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adenocarcinoma

characteristic: disease

text: Mus musculus

text: Mus musculus

text: lung epithelium

characteristic: organism part

text: p-ERK High

characteristic: phenotype

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 8


E-MTAB-6432:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BAH-GU

characteristic: INSDC center name

text: public

text: E-MTAB-6432:Sample 1

text: ArrayExpress

text: house mouse

text: adenocarcinoma

text: Mus musculus

text: Mus musculus

text: lung epithelium

text: p-ERK Low

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: FVBN x C57BL/6

characteristic: strain

text: Sample 1


E-MTAB-6432:Sample 3

text: BAH-GU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6432:Sample 3

text: ArrayExpress

text: house mouse

characteristic: description

text: adenocarcinoma

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lung epithelium

text: p-ERK Low

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 3


E-MTAB-6432:Sample 5

characteristic: ENA-CHECKLIST

text: BAH-GU

text: public

text: E-MTAB-6432:Sample 5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: house mouse

characteristic: common name

text: adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung epithelium

text: p-ERK Low

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 5

characteristic: title


E-MTAB-6432:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BAH-GU

text: public

text: E-MTAB-6432:Sample 7

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adenocarcinoma

characteristic: disease

text: Mus musculus

text: Mus musculus

text: lung epithelium

characteristic: organism part

text: p-ERK Low

characteristic: phenotype

text: neoplasm

text: Mus musculus

text: FVBN x C57BL/6

text: Sample 7


characteristic: External Id

text: LOUISIANA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:LOUISIANA STATE UNIVERSITY

text: San Diego copepods exposed to 15psu

text: Tigriopus californicus

text: Tigriopus californicus


text: LOUISIANA STATE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: San Diego copepods exposed to 35psu

characteristic: description

text: Tigriopus californicus

text: Tigriopus californicus


characteristic: ENA-CHECKLIST

text: LOUISIANA STATE UNIVERSITY

text: public

characteristic: Submitter Id

text: San Diego copepods exposed to 60psu

text: Tigriopus californicus

text: Tigriopus californicus


text: LOUISIANA STATE UNIVERSITY

text: public

text: Bodega Reserve copepods exposed to 15psu

text: Tigriopus californicus

characteristic: organism

text: Tigriopus californicus


text: LOUISIANA STATE UNIVERSITY

text: public

text: Bodega Reserve copepods exposed to 35psu

text: Tigriopus californicus

text: Tigriopus californicus

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: LOUISIANA STATE UNIVERSITY

text: public

text: Bodega Reserve copepods exposed to 60psu

text: Tigriopus californicus

text: Tigriopus californicus


characteristic: ENA-LAST-UPDATE

text: INALI, CONICET-UNL

text: public

tag: Namespace:INALI, CONICET-UNL

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: Environmental surface sample from freshwater floodplain river

text: Pampa, eco-region delta and islands of the Parana

characteristic: environment (biome)

text: floodplain stream

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Yes

characteristic: environmental sample

characteristic: further details

text: Argentina

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

text: F_5'-CCAGCASCYGCGGTAATTCC-3' R_5'-ACTTTCGTTCTTGATYRATGA-3'

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

text: Water sample from Correntoso Stream located at the Paran� River floodplain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: INALI, CONICET-UNL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured eukaryote

text: Environmental surface sample from freshwater floodplain pond

characteristic: description

text: floodplain lake

text: water

text: Yes

text: Argentina

text: uncultured eukaryote

characteristic: organism

text: uncultured eukaryote

text: Water sample from Correntoso pond located at the Paran� River floodplain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: INALI, CONICET-UNL

text: public

characteristic: collection date

text: uncultured eukaryote

characteristic: common name

text: Environmental surface sample from freshwater floodplain lake

characteristic: environment (biome)

text: floodplain lake

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Yes

characteristic: environmental sample

characteristic: further details

text: Argentina

text: uncultured eukaryote

text: uncultured eukaryote

text: Water sample from Del Medio Lake located at the Paran� River floodplain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: INALI, CONICET-UNL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: uncultured eukaryote

text: floodplain lake

text: water

text: Yes

text: Argentina

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: uncultured eukaryote

characteristic: pcr primers

text: uncultured eukaryote

characteristic: target gene

characteristic: target subfragment

text: Water sample from Ferranda Lake located at the Paran� River floodplain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SOUTHERN MEDICAL UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:SOUTHERN MEDICAL UNIVERSITY

characteristic: Submitter Id

characteristic: collection date

text: house mouse

characteristic: common name

text: China

text: healthy

characteristic: host health state

text: Mus musculus

characteristic: host scientific name

text: fecal

characteristic: isolate

text: fecal microbiota transplantation

characteristic: isolation source

text: Mus musculus

characteristic: organism

text: Mus musculus

text: FMT


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF NEW SOUTH WALES

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:UNIVERSITY OF NEW SOUTH WALES

text: marine toad

text: High molecular weight DNA extraction from Cane toad (Rhinella marina) liver for whole genome sequencing

characteristic: description

text: Australia

characteristic: geographic location (country and/or sea)

text: Forrest River, Oombulgurri

characteristic: geographic location (region and locality)

text: 15.1818S, 127.8413E

characteristic: lat lon

text: Rhinella marina

text: Rhinella marina

text: Female

characteristic: sex

text: Liver

characteristic: tissue type

text: Cane toad (Rhinella marina) liver

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Instituto de Tecnologia Quimica e Biologica Antonio Xavier

characteristic: Submitter Id

characteristic: collection date

text: USA

text: diseased

text: Homo sapiens

text: NY

text: Staphylococcus aureus

text: Staphylococcus aureus

characteristic: strain

text: USA300 RU-CAMP-18


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Instituto de Tecnologia Quimica e Biologica Antonio Xavier

characteristic: INSDC center name

text: public

text: USA

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: laboratory mutant

characteristic: isolation source

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: USA300 high-level oxacillin resistant strain


characteristic: INSDC first public

text: public

text: USA

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

text: NY

text: Staphylococcus aureus

text: Staphylococcus aureus

text: USA300 RU-CAMP-19


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: USA

text: diseased

text: Homo sapiens

text: NY

text: Staphylococcus aureus

text: Staphylococcus aureus

text: USA300 RU-CAMP-28

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: USA

text: diseased

text: Homo sapiens

text: NY

text: Staphylococcus aureus

text: Staphylococcus aureus

text: USA300 RU-CAMP-29


text: public

tag: Namespace:Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University

characteristic: Submitter Id

text: Micropruina glycogenica

text: Micropruina glycogenica

text: Micropruina glycogenica Lg2


characteristic: ENA-CHECKLIST

text: IMPERIAL COLLEGE LONDON

text: public

tag: Namespace:IMPERIAL COLLEGE LONDON

characteristic: collection date

text: Streptococcus pneumoniae RM200 Rx1E PdT ΔlytA

characteristic: description

text: USA

text: Homo sapiens

text: Boston Children's Hospital

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae RM200


characteristic: External Id

characteristic: INSDC center name

text: public

text: metagenomes

text: metagenomes

text: Microbial biogeography of French caves


text: public

tag: Namespace:Oxford University Clinical Research Unit, Viet Nam

text: Cambodia

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: Klebsiella pneumoniae

characteristic: isolate

text: carriage

characteristic: isolation source

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: INSDC first public

text: public

tag: Namespace:Center for Quantitative Genetics and Genomics

text: Neovison vison

characteristic: organism

text: Neovison vison

text: gDNA


characteristic: ENA-FIRST-PUBLIC

text: Center for Quantitative Genetics and Genomics

text: public

text: Neovison vison

text: Neovison vison

text: gDNA


ga

characteristic: ENA-LAST-UPDATE

text: public

text: ga

text: Neovison vison

text: Neovison vison

text: gDNA


gb

text: public

text: gb

text: Neovison vison

text: Neovison vison

text: gDNA

characteristic: title


go

characteristic: INSDC last update

text: public

text: go

text: Neovison vison

text: Neovison vison

text: gDNA


m

text: public

characteristic: INSDC status

text: m

text: Neovison vison

text: Neovison vison

text: gDNA


text: public

characteristic: SRA accession

tag: Namespace:WAGENINGEN UNIVERSITY, LABORATORY OF SYSTEMS AND SYNTHETIC BIOLOGY.

text: Aspergillus niger NW186 (N402 derivative) grown with 1.1 mM arginine supplement.

text: Aspergillus niger

text: Aspergillus niger

text: NW186 arginine 1


text: WAGENINGEN UNIVERSITY, LABORATORY OF SYSTEMS AND SYNTHETIC BIOLOGY.

text: public

characteristic: Submitter Id

text: Aspergillus niger

text: Aspergillus niger

text: NW186 arginine 2


characteristic: ENA-CHECKLIST

text: public

text: Aspergillus niger NW186 (N402 derivative) grown with 5 mM citrulline supplement.

text: Aspergillus niger

text: Aspergillus niger

text: NW186 citrulline 1


text: public

text: Aspergillus niger

text: Aspergillus niger

text: NW186 citrulline 2


1 parental PBMNCs

text: Cell therapy center

text: public

text: 1 parental PBMNCs

tag: Namespace:Cell therapy center

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Human induced neural stem cell lines and parental PBMNCs whole genome sequencing


2 episomal iNSC P43

text: Cell therapy center

text: public

text: 2 episomal iNSC P43

text: human

text: Homo sapiens

text: Homo sapiens


3 Sev iNSC1 P27

text: Cell therapy center

text: public

text: 3 Sev iNSC1 P27

text: human

text: Homo sapiens

text: Homo sapiens


4 Sev iNSC2 P22

text: Cell therapy center

text: public

text: 4 Sev iNSC2 P22

text: human

text: Homo sapiens

text: Homo sapiens


text: Yeast

characteristic: ArrayExpress-PHENOTYPE

text: Saccharomyces cerevisiae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Yeast

characteristic: phenotype

text: Saccharomyces cerevisiae

characteristic: subject id


Illumina MiSeq reads

characteristic: External Id

text: JIC

characteristic: INSDC center name

text: public

text: Illumina MiSeq reads

text: Streptomyces cinnamoneus

text: Streptomyces cinnamoneus

text: S. cinnamoneus DSM 41675


Nanopore reads

text: JIC

characteristic: INSDC first public

text: public

text: Nanopore reads

text: Streptomyces cinnamoneus

text: Streptomyces cinnamoneus

text: S. cinnamoneus DSM 41675


text: synthetic construct

text: SC

text: public

text: synthetic construct

text: synthetic construct

characteristic: organism

text: synthetic construct


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute

text: ArrayExpress

characteristic: broker name

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: Cells were grown in regular DMEM or in DMEM lacking Met/Cys for different time points (6-30-120 hours for regular DMEM or 6-15-30-72-120 hours for medium w/o Met/Cys) . Cells were trypsinized and collected by centrifugation, then immediately frozen at -80°C. Total RNA was prepared using Trizol (Sigma), to preserve transcripts of both small and long sizes, and DNase treated to avoid contamination of genomic DNA. Ovation RNA-Seq System V2

characteristic: description

text: cervical adenocarcinoma

characteristic: disease

characteristic: genotype

text: DMEM without methionine or cysteine

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

characteristic: common name

text: cervical adenocarcinoma

text: DMEM without methionine or cysteine

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: normal DMEM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens


E-MTAB-6451:Sample 1

text: public

text: E-MTAB-6451:Sample 1

tag: Namespace:Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital, London, UK

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Lymph node samples were homogenized in 600ul RLT lysis buffer (Qiagen) using a Precellys Homogenizer Precellys. RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. RNA integrity was confirmed using Agilent’s 2100 Bioanalyser. Libraries were prepared using the KAPA mRNA Hyper Prep Kit (Roche), following manufacturer’s instructions.

characteristic: description

text: normal

characteristic: disease

text: Il7fx/KO Rosa26CreERT2

text: Mus musculus

text: Mus musculus

text: lymph node

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-6451:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital, London, UK

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6451:Sample 2

text: ArrayExpress

text: house mouse

text: normal

text: Il7fx/KO Rosa26CreERT2

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: male

text: Sample 2


E-MTAB-6451:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-6451:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: normal

text: Il7fx/KO Rosa26CreERT2

text: Mus musculus

text: Mus musculus

text: lymph node

text: wild type phenotype

text: Mus musculus

text: male

text: Sample 3

characteristic: title


E-MTAB-6451:Sample 4

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6451:Sample 4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: normal

text: Il7fx/KO Rosa26CreERT2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: Ilf7 knockout

text: Mus musculus

text: male

text: tamoxifen

characteristic: stimulus

text: Sample 4


E-MTAB-6451:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: E-MTAB-6451:Sample 5

text: ArrayExpress

text: house mouse

text: normal

characteristic: disease

text: Il7fx/KO Rosa26CreERT2

text: Mus musculus

text: Mus musculus

text: lymph node

text: Ilf7 knockout

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: tamoxifen

characteristic: strain

text: Sample 5


E-MTAB-6451:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6451:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: normal

text: Il7fx/KO Rosa26CreERT2

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: Ilf7 knockout

text: Mus musculus

text: male

text: tamoxifen

text: Sample 6


E-MTAB-6453:Mucor circinelloides

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6453:Mucor circinelloides

tag: Namespace:Station biologique de Roscoff, plateforme ABiMS,

text: ArrayExpress

characteristic: broker name

text: Protocols: Strains were grown on Potato Dextrose Agar (PDA) at 25°C during 7 days. Total RNA extraction was done using the RNeasy plant mini kit (Qiagen, Courtaboeuf, France) following the manufacturer's instructions. The RNA-seq libraries were prepared from total RNA using the Illumina Ribo-Zero rRNA Removal Kit (Epicenter,Madison, WI) and TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA) following the manufacturer's instructions.

characteristic: description

text: potato dextrose agar at 25C during 7 days

characteristic: growth condition

text: Mucor circinelloides

text: Mucor circinelloides

text: Mucor circinelloides

text: CBS 277-49

text: Mucor circinelloides

characteristic: title


E-MTAB-6453:Mucor endophyticus

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Station biologique de Roscoff, plateforme ABiMS,

characteristic: INSDC last update

text: public

text: E-MTAB-6453:Mucor endophyticus

text: ArrayExpress

text: Mucor endophyticus

characteristic: organism

text: Mucor endophyticus

text: Mucor endophyticus

text: CBS 385-95

text: Mucor endophyticus


E-MTAB-6453:Mucor fuscus

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6453:Mucor fuscus

text: ArrayExpress

text: Mucor fuscus

text: Mucor fuscus

text: Mucor fuscus

text: UBOCC -A-109160

characteristic: strain

text: Mucor fuscus


E-MTAB-6453:Mucor lanceolatus

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-6453:Mucor lanceolatus

text: ArrayExpress

text: Mucor lanceolatus

text: Mucor lanceolatus

text: Mucor lanceolatus

text: Mucor lanceolatus


E-MTAB-6453:Mucor racemosus

text: public

characteristic: SRA accession

text: E-MTAB-6453:Mucor racemosus

characteristic: Submitter Id

text: ArrayExpress

text: Mucor racemosus

text: Mucor racemosus

text: Mucor racemosus

text: Mucor racemosus


text: PhD student

text: public

tag: Namespace:PhD student

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. sacrificed at 5th instar sacrificed at pupal stage Sacrificed at adult stage Short day treatment (12 hours daylight + 12 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: third instar larva stage

characteristic: developmental stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: unkown

characteristic: sex

text: Swedish


text: PhD student

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. sacrificed at 5th instar sacrificed at pupal stage Sacrificed at adult stage long-day (22 hours daylight + 2 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: third instar larva stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: unkown

text: Swedish


text: PhD student

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: third instar larva stage

text: long-day (22 hours daylight + 2 hours dark)

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: unkown

text: Swedish


text: PhD student

text: public

text: ArrayExpress

text: third instar larva stage

text: short-day (12 hours daylight + 12 hours dark)

characteristic: growth condition

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: unkown

text: Swedish

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: PhD student

text: public

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. Sacrificed at instar 3 sacrificed at 5th instar sacrificed at pupal stage Short day treatment (12 hours daylight + 12 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: adult

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: female

text: Swedish


text: PhD student

characteristic: INSDC last update

text: public

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. Sacrificed at instar 3 sacrificed at 5th instar Sacrificed at adult stage Short day treatment (12 hours daylight + 12 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: pupa

text: Leptidea sinapis

characteristic: organism

text: Leptidea sinapis

text: Leptidea sinapis

text: female

text: Swedish


characteristic: ENA-FIRST-PUBLIC

text: PhD student

text: public

text: ArrayExpress

text: pupa

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: male

text: Swedish

characteristic: strain


characteristic: External Id

text: PhD student

text: public

characteristic: INSDC status

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. Sacrificed at instar 3 sacrificed at pupal stage Sacrificed at adult stage Short day treatment (12 hours daylight + 12 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: fifth instar larva stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: female

text: Swedish


text: PhD student

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fifth instar larva stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: male

text: Swedish


text: PhD student

text: public

text: ArrayExpress

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. Sacrificed at instar 3 sacrificed at 5th instar Sacrificed at adult stage long-day (22 hours daylight + 2 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

text: pupa

characteristic: developmental stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: female

characteristic: sex

text: Swedish


text: PhD student

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: pupa

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: male

text: Swedish


text: PhD student

text: public

text: ArrayExpress

characteristic: broker name

text: Protocols: Three mated female L. sinapis (Swedish ecotype) were collected in south central Sweden (Uppsala region) during spring 2016 and kept in captivity in separate 50*50*50 cm cages in a common garden with constant day light (18 hours light, 6 hours dark) and access to sugar water and stands of a commonly utilized host plant, birdsfoot trefoil (Lotus corniculatus), for egg laying. Eggs collected from each of the three unrelated females (families, from here on) were divided into two cohorts and reared in growth chambers with constant temperature (23degreesC), under either short-day (12 hours daylight + 12 hours dark) or long-day (22 hours daylight + 2 hours dark) light conditions (Supplementary Figure 1). Lights-off switch time was synchronized between treatments. Based on previous data in other butterfly species with a pupal overwintering stage, including the related pierid, green-veined white (Pieris napi), the critical point for diapause or direct development induction takes place during larval instar-IV (Friberg et al. 2011). Developing individuals were therefore monitored daily and samples were harvested the day after molting into larval stages instar-III and instar-V, and one day after pupation. Adult samples were also collected for the long-day light treatment the day after eclosion. Apart from instar-III, six samples (three males and three females, one from each family) were collected for each developmental stage and light treatment. Only three samples were collected for instar-III larvae (one from each family) since sexing based on morphology is impracticable at that stage (Friberg & Wiklund, personal observation). Hence, in total 36 samples were harvested representing three (short-day treatment) or four (long-day treatment) developmental stages (Supplementary Figure 1). At least ten additional individuals from the same families were raised under each light treatment to control that short-day and long-day treatments triggered diapause induction and direct development, respectively. All ten individuals raised under long-day light conditions went into direct development and eclosed after 7-10 days as pupae. All ten individuals reared under short-day light conditions entered diapause upon arriving to the pupal stage (and remained in diapause for several months). whole body of the butterfly at the particular stage was used for the analysis. Sacrificed at instar 3 sacrificed at pupal stage Sacrificed at adult stage long-day (22 hours daylight + 2 hours dark) Total RNA was extracted from the whole body using a modified acid phenol-chloroform protocol (Chomczynski & Mackey 1995; Chomczynski & Sacchi 1987). Individual libraries were generated using the Illumina TruSeq Stranded mRNA protocol (Illumina Inc.) with Poly-A selection and using dUTP for second strand cDNA synthesis.

characteristic: description

text: fifth instar larva stage

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: female

text: Swedish


text: PhD student

text: public

text: ArrayExpress

text: fifth instar larva stage

characteristic: growth condition

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: male

text: Swedish

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: PhD student

text: public

text: ArrayExpress

text: adult

text: Leptidea sinapis

text: Leptidea sinapis

text: Leptidea sinapis

text: male

text: Swedish


text: PhD student

characteristic: INSDC last update

text: public

text: ArrayExpress

text: pupa

text: Leptidea sinapis

characteristic: organism

text: Leptidea sinapis

text: Leptidea sinapis

text: unkown

text: Swedish


characteristic: ENA-FIRST-PUBLIC

text: EAWAG

text: public

characteristic: cyclekit

text: Real bath toy

text: ACTCGCTA

text: TCGACTAG

text: biofilm metagenome

characteristic: origin

characteristic: rundate

text: biofilm metagenome

text: Bathtoy


characteristic: External Id

text: EAWAG

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Real bath toy

text: ACTCGCTA

text: TTCTAGCT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Real bath toy

text: ACTCGCTA

text: CCTAGAGT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

characteristic: description

text: ACTCGCTA

text: GCGTAAGA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: ACTCGCTA

text: CTATTAAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: ACTCGCTA

text: AAGGCTAT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: ACTCGCTA

text: GAGCCTTA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: ACTCGCTA

text: TTATGCGA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: TCGACTAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: TTCTAGCT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: CCTAGAGT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: GCGTAAGA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: CTATTAAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: AAGGCTAT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: GAGCCTTA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GGAGCTAC

text: TTATGCGA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GCGTAGTA

text: TCGACTAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy

text: GCGTAGTA

text: TTCTAGCT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


Clean water control_1

text: EAWAG

text: public

text: Clean water control_1

text: Control bath toy - experimental replicate

text: GCGTAGTA

text: CCTAGAGT

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


Clean water control_2

text: EAWAG

text: public

text: Clean water control_2

text: GCGTAGTA

text: GCGTAAGA

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


Clean water control_3

text: EAWAG

text: public

text: Clean water control_3

text: GCGTAGTA

text: CTATTAAG

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


Dirty water control_1

text: EAWAG

text: public

text: Dirty water control_1

text: GCGTAGTA

text: AAGGCTAT

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


Dirty water control_2

text: EAWAG

text: public

text: Dirty water control_2

text: GCGTAGTA

text: GAGCCTTA

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


Dirty water control_3

text: EAWAG

text: public

text: Dirty water control_3

text: GCGTAGTA

text: TTATGCGA

text: biofilm metagenome

text: Control

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TGCAGCTA

text: CTATTAAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TGCAGCTA

text: AAGGCTAT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TCGACGTC

text: TCGACTAG

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TCGACGTC

text: TTCTAGCT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TCGACGTC

text: CCTAGAGT

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: EAWAG

text: public

text: Real bath toy - amplification replicate

text: TCGACGTC

text: GCGTAAGA

text: biofilm metagenome

text: biofilm metagenome

text: Bathtoy


text: YALE

text: public

text: healthy donor 1 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 2 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 4 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 5 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 6 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 7 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 8 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 9 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: subacute cutaneous lupus erythematosus donor 1 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 8 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 9 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 1 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 1 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 4 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 6 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 7 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 8 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 2 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 1 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 4 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 6 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 8 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 3 buccal swab

text: human oral metagenome

text: human oral metagenome


text: YALE

text: public

text: healthy donor 1 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 4 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 6 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 7 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 8 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: subacute cutaneous lupus erythematosus donor 1 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 8 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 9 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 1 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 1 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 4 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 6 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 7 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 8 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 2 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 8 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 3 chest skin swab

text: human skin metagenome

text: human skin metagenome


text: YALE

text: public

text: healthy donor 1 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 2 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 4 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 5 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 6 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 7 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 8 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: subacute cutaneous lupus erythematosus donor 1 visit 1 chest skin swab

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 6 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 8 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 9 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 1 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 1 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 4 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: healthy donor 6 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 7 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: healthy donor 8 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 2 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 4 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 2 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 2 fecal

text: human gut metagenome

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text: YALE

text: public

text: healthy donor 1 visit 3 fecal

text: human gut metagenome

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text: YALE

text: public

text: healthy donor 4 visit 3 fecal

text: human gut metagenome

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text: YALE

text: public

text: systemic lupus erythematosus donor 1 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 3 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 5 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 7 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 10 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 11 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: YALE

text: public

text: systemic lupus erythematosus donor 12 visit 3 fecal

text: human gut metagenome

text: human gut metagenome


text: public

tag: Namespace:Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

text: Protocols: Plants were grown in a plant growth room at 21 degree C, 60% relative humidity, long day (16h light/ 8h dark) Collection of floral organs from growth-room grown Arabidopsis thaliana plants. Flowers were emasculated at flower stage 12c to prevent self-pollination, and harvested at the three indicated time points. For tissue dissection, flowers were removed from the plant and placed under a standard binocular dissection microscope. With the tip of a sterile hypodermic needle, the stigma was carefully removed from the flower, while trying to minimize contamination by the underlying style tissue, and immediately flash-frozen. For each biological replicate, about 200 stigmata were pooled. RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich). RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyzer 2100 (Agilent). Per sample, an amount of 100 ng of total RNA was used as input. Using the Illumina TruSeq® Stranded mRNA Sample Prep Kit (protocol version: Part # 15031047 Rev. D - September 2012) poly-A containing mRNA molecules were purified from the total RNA input using poly-T oligo-attached magnetic beads. In a reverse transcription reaction using random primers, RNA was converted into first strand cDNA and subsequently converted into double-stranded cDNA in a second strand cDNA synthesis reaction using DNA PolymeraseI and RNAse H. The cDNA fragments were extended with a single ‘A’ base to the 3’ ends of the blunt-ended cDNA fragments after which multiple indexing adapters were ligated introducing different barcodes for each sample. Finally, enrichment PCR was carried out to enrich those DNA fragments that have adapter molecules on both ends and to amplify the amount of DNA in the library. Sequence-libraries of each sample were equimolarly pooled.

text: whole plant flowering stage

characteristic: developmental stage

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: stigma

characteristic: organism part

text: Arabidopsis thaliana


tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium

characteristic: INSDC last update

text: public

text: ArrayExpress

text: thale cress

text: whole plant flowering stage

text: wild type genotype

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: stigma

text: Arabidopsis thaliana

characteristic: title


name: E-MTAB-6456:ODN1, Normal PCR, dATP dilution 1, r1

characteristic: External Id

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Chemistry University of Cambridge

characteristic: Submitter Id

text: ArrayExpress

unit: micromolar

characteristic: dATP dilution concentration

text: Protocols: ODN 1: NNNNNNNNNNATCGAGAATCCCGGTGCCGATACCXACTCTTGYAGAANNNNNNNNNNCGTGTCAGATATATACATCCGAT (N: random ATGC, X and Y: 5hmU); The template ODN was purchased from ATDbio. The double stranded DNA was synthesized by single-extension PCR using 5'-ATCGGATGTATATATCTGACACG-as the primer. ODN 2: GCTCGCTTTGTTGGTTTCCTTGTTCTCTGTGCCCACTGCCTGACGGGCGGAAAGCAGCGCGAGCAAGCGAGACAGGACAC; The template ODN was purchased from Life Technologies. The double-stranded DNA was prepared by single-extension PCR using 5'-GTGTCCTGTCTCGCTTGCTCGCGCTGCTTT-3' as the primer where TTP (10 mM) was substituted by a mixture of TTP and 5hmdUTP (Trilink biotechnologies) in a ratio of 0:1, 1:1, 3:1, 7:1, 15:1, 1:0 to obtain fully, or partially 5hmU modified ODN pools. ODN 3: CTGTGGCTCTGCGTCCTTGTCCTNNNNNNACACAGCGCAN5NGAACGACGAGGCACAACAGAGAGCAACACCGCCGAGGA (N: random A or T or G or C, 5:5hmU); The template ODN was purchased from ATDbio. The double stranded DNA was synthesized by single-extension PCR using 5'-TCCTCGGCGGTGTTGCTCTCTGTTGTGCCT-3'as the primer. Purified by DNA Clean & Concentrator™-5 (Zymo Research). Detailed information is available in the liked publication. Briefly, the DNA was ligated with a custom adapter partially bearing Truseq LT adapter (Illumina) sequences, followed by KRuO4-mediated oxidation to oxidise 5hmU. Then single extension PCR was carried out to create libraries with and full length Truseq adapter sequence as well as information of 5hmU locations. The libraries were then amplified by PCR (6-8 cycles).

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct

text: ODN1, Normal PCR, dATP dilution 1, r1


name: E-MTAB-6456:ODN1, Normal PCR, dATP dilution 1, r2

text: Department of Chemistry University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN1, Normal PCR, dATP dilution 1, r2


name: E-MTAB-6456:ODN1, Normal PCR, dATP dilution 1, r3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN1, Normal PCR, dATP dilution 1, r3


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, dATP dilution 50, r1

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

unit: millimolar

characteristic: Mg2SO4 concentration

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, dATP dilution 50, r2

characteristic: External Id

text: public

characteristic: INSDC status

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

characteristic: title


name: E-MTAB-6456:ODN1, Bst, 37 deg Tpol, 2 mM MgSO4, dATP dilution 500, r1

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

unit: nanomolar

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg Tpol, 2 mM MgSO4, dATP dilution 500, r2

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg Tpol, 2 mM MgSO4, dATP dilution 500, r3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, dATP dilution 500, r1

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, dATP dilution 500, r2

characteristic: External Id

text: public

characteristic: INSDC status

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

characteristic: title


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, dATP dilution 500, r3

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, nooxctrl, dATP dilution 500, r1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

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characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, nooxctrl, dATP dilution 500, r2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct


name: E-MTAB-6456:ODN1, Bst, 37 deg, Tpol, 10 mM MgSO4, nooxctrl, dATP dilution 500, r3

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct


name: E-MTAB-6456:ODN1, Klenow exo-, 37 deg, Tpol, dATP dilution 500, r1

characteristic: External Id

text: public

characteristic: INSDC status

text: ArrayExpress

text: Klenow Fragment (3'-5' exo-)

text: synthetic construct

characteristic: organism

text: synthetic construct

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text: synthetic construct

characteristic: title


name: E-MTAB-6456:ODN1, Klenow exo-, 37 deg, Tpol, dATP dilution 500, r2

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Klenow Fragment (3'-5' exo-)

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, Sulfolobus pol, 37deg, Tpol, 10 mM MgSO4, dATP dilution 500, r1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Sulfolobus DNA Polymerase IV

text: synthetic construct

text: synthetic construct

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characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct


name: E-MTAB-6456:ODN1, Sulfolobus pol, 37deg, Tpol, 10 mM MgSO4, dATP dilution 500, r3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Sulfolobus DNA Polymerase IV

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, VentR exo-, 37deg, Tpol, 10 mM MgSO4, dATP dilution 500, r1

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characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: ArrayExpress

text: Vent (exo-) DNA Polymerase

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct


name: E-MTAB-6456:ODN1, VentR exo-, 37deg, Tpol, 10 mM MgSO4, dATP dilution 500, r2

characteristic: External Id

text: public

characteristic: INSDC status

text: ArrayExpress

text: Vent (exo-) DNA Polymerase

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

characteristic: title


E-MTAB-6456:ODN2, 0%, ox, r1

text: public

characteristic: SRA accession

text: E-MTAB-6456:ODN2, 0%, ox, r1

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

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text: ox

text: synthetic construct

text: ODN2, 0%, ox, r1


E-MTAB-6456:ODN2, 0%, ox, r2

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6456:ODN2, 0%, ox, r2

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct

text: ODN2, 0%, ox, r2


E-MTAB-6456:ODN2, 0%, ox, r3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6456:ODN2, 0%, ox, r3

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 0%, ox, r3


E-MTAB-6456:ODN2, 12%, ox, r1

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: E-MTAB-6456:ODN2, 12%, ox, r1

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 12%, ox, r1


E-MTAB-6456:ODN2, 12%, ox, r2

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-6456:ODN2, 12%, ox, r2

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

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characteristic: organism

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text: ODN2, 12%, ox, r2

characteristic: title


E-MTAB-6456:ODN2, 12%, ox, r3

text: public

characteristic: SRA accession

text: E-MTAB-6456:ODN2, 12%, ox, r3

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 12%, ox, r3


E-MTAB-6456:ODN2, 25%, nooxctrl, r1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6456:ODN2, 25%, nooxctrl, r1

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: nooxctrl

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct

text: ODN2, 25%, nooxctrl, r1


E-MTAB-6456:ODN2, 25%, nooxctrl, r2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6456:ODN2, 25%, nooxctrl, r2

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct

text: ODN2, 25%, nooxctrl, r2


E-MTAB-6456:ODN2, 25%, nooxctrl, r3

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: E-MTAB-6456:ODN2, 25%, nooxctrl, r3

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct

text: ODN2, 25%, nooxctrl, r3


E-MTAB-6456:ODN2, 25%, ox, r1

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-6456:ODN2, 25%, ox, r1

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 25%, ox, r1

characteristic: title


E-MTAB-6456:ODN2, 25%, ox, r2

text: public

characteristic: SRA accession

text: E-MTAB-6456:ODN2, 25%, ox, r2

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 25%, ox, r2


E-MTAB-6456:ODN2, 25%, ox, r3

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6456:ODN2, 25%, ox, r3

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct

text: ODN2, 25%, ox, r3


E-MTAB-6456:ODN2, 50%, ox, r1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6456:ODN2, 50%, ox, r1

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 50%, ox, r1


E-MTAB-6456:ODN2, 50%, ox, r2

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: E-MTAB-6456:ODN2, 50%, ox, r2

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 50%, ox, r2


E-MTAB-6456:ODN2, 50%, ox, r3

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-6456:ODN2, 50%, ox, r3

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

text: ODN2, 50%, ox, r3

characteristic: title


E-MTAB-6456:ODN3, nooxctrl, r1

text: public

characteristic: SRA accession

text: E-MTAB-6456:ODN3, nooxctrl, r1

characteristic: Submitter Id

text: ArrayExpress

characteristic: dATP dilution concentration

text: Bst DNA Polymerase, Large Fragment

characteristic: oligo

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct

text: ODN3, nooxctrl, r1


E-MTAB-6456:ODN3, nooxctrl, r2

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6456:ODN3, nooxctrl, r2

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: nooxctrl

characteristic: oxidation treatment

characteristic: replicate

text: synthetic construct

text: ODN3, nooxctrl, r2


E-MTAB-6456:ODN3, nooxctrl, r3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-6456:ODN3, nooxctrl, r3

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

text: synthetic construct

text: nooxctrl

text: synthetic construct

text: ODN3, nooxctrl, r3


E-MTAB-6456:ODN3, ox, r1

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: Mg2SO4 concentration

text: E-MTAB-6456:ODN3, ox, r1

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

characteristic: enzyme

text: synthetic construct

text: synthetic construct

text: ox

text: synthetic construct

text: ODN3, ox, r1


E-MTAB-6456:ODN3, ox, r2

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-6456:ODN3, ox, r2

text: ArrayExpress

text: Bst DNA Polymerase, Large Fragment

text: synthetic construct

characteristic: organism

text: synthetic construct

text: ox

text: synthetic construct

text: ODN3, ox, r2

characteristic: title