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characteristic: elevation

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characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

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text: public

tag: Namespace:South African Sugarcane Research Institute

text: T R Williams

characteristic: collected by

characteristic: collection date

text: European white birch

characteristic: common name

text: Using 11 novel PCR primers, the complete sequence of Betula pendula was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Gwynedd, North Wales

characteristic: geographic location (region and locality)

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characteristic: identified by

text: 52.924816 -4.444735

characteristic: lat lon

text: Betula pendula

characteristic: organism

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text: Chloroplast isolation, amplification, sequencing and assembly of Betula pendula.

characteristic: title


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text: plastid:chloroplast

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text: Chloroplast isolation, amplification, sequencing and assembly of Fagus sylvatica


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text: Using 11 novel PCR primers, the complete sequence of Malus sylvestris was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

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text: D Lloyd Evans

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text: Malus sylvestris

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text: Chloroplast isolation, amplification, sequencing and assembly of Malus sylvestris


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characteristic: collection date

text: apple

characteristic: common name

text: Cox's Orange Pippin

characteristic: cultivar

text: Using 11 novel PCR primers, the complete sequence of Malus domestica cv Cox's Orange Pippin was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Gwynedd, North Wales

characteristic: geographic location (region and locality)

text: D Lloyd Evans

characteristic: identified by

text: 52.924816 -4.444735

text: Malus domestica

text: Malus domestica

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Malus domestica cv Cox's Orange Pippin


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: T R Williams

text: Scots pine

text: Using 11 novel PCR primers, the complete sequence of Pinus sylvestris was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

characteristic: lat lon

text: Pinus sylvestris

characteristic: organism

text: Pinus sylvestris

text: young leaf

characteristic: tissue type

text: Chloroplast isolation, amplification, sequencing and assembly of Pinus sylvestris

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: V Beaumont

text: Using 11 novel PCR primers, the complete sequence of Populus nigra was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Burgh St. Peter, Suffolk

text: D Lloyd Evans

text: 52.484293 1.630794

text: Populus nigra

text: Populus nigra

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Populus nigra


characteristic: INSDC first public

text: public

text: T R Williams

characteristic: collected by

characteristic: collection date

text: sweet cherry

characteristic: common name

text: Using 11 novel PCR primers, the complete sequence of Prunus avium was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

characteristic: description

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

text: Prunus avium

text: Prunus avium

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Prunus avium


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: T R Williams

text: pear

text: Bartlett

characteristic: cultivar

text: Using 11 novel PCR primers, the complete sequence of Pyrus communis cv Bartlett was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Gwynedd, North Wales

characteristic: geographic location (region and locality)

text: D Lloyd Evans

characteristic: identified by

text: 52.924816 -4.444735

text: Pyrus communis

text: Pyrus communis

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Pyrus communis cv Bartlett


characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: T R Williams

text: sessile oak

text: Using 11 novel PCR primers, the complete sequence of Quercus petrea was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

characteristic: lat lon

text: Quercus petraea

characteristic: organism

text: Quercus petraea

text: leaf

characteristic: tissue type

text: Chloroplast isolation, amplification, sequencing and assembly of Quercus petrea

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: South African Sugarcane Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: T R Williams

text: Using 11 novel PCR primers, the complete sequence of Quercus robur was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

text: Quercus robur

text: Quercus robur

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Quercus robur


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: T R Williams

characteristic: collected by

characteristic: collection date

text: Using 11 novel PCR primers, the complete sequence of Rosa canina was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

characteristic: description

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

text: Rosa canina

text: Rosa canina

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Rosa canina


characteristic: ENA-FIRST-PUBLIC

text: public

text: T R Williams

text: osier

characteristic: common name

text: Using 11 novel PCR primers, the complete sequence of Salix viminalis was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Gwynedd, North Wales

characteristic: geographic location (region and locality)

text: D Lloyd Evans

characteristic: identified by

text: 52.924816 -4.444735

text: Salix viminalis

text: Salix viminalis

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Salix viminalis


text: public

characteristic: INSDC status

text: T R Williams

text: English yew

text: Using 11 novel PCR primers, the complete sequence of Taxus baccata was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Gwynedd, North Wales

text: D Lloyd Evans

text: 52.924816 -4.444735

characteristic: lat lon

text: Taxus baccata

characteristic: organism

text: Taxus baccata

text: leaf

characteristic: tissue type

text: Chloroplast isolation, amplification, sequencing and assembly of Taxus baccata

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: V Beaumont

text: Using 11 novel PCR primers, the complete sequence of Viburnum opulus was sequenced with the Oxford Nanopore platform. Reads were assembled with canu and error corrected with nanopolish.

text: United Kingdom

text: Burgh St. Peter, Suffolk

text: D Lloyd Evans

text: 52.484293 1.630794

text: Viburnum opulus

text: Viburnum opulus

text: leaf

text: Chloroplast isolation, amplification, sequencing and assembly of Viburnum opulus


characteristic: ENA-CHECKLIST

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: INSDC last update

text: public

tag: Namespace:INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: Submitter Id

text: Daphnia magna

text: Daphnia magna

text: Daphnia magna microRNAs respond to nutritional stress and ageing but are not transgenerational


characteristic: ENA-FIRST-PUBLIC

text: Beijing Chaoyang Hospita

text: public

tag: Namespace:Beijing Chaoyang Hospita

characteristic: collection date

text: blood plasma

characteristic: environment (biome)

text: blood plasma

characteristic: environment (feature)

text: blood plasma

characteristic: environment (material)

text: China

text: 39.9 N

unit: DD

characteristic: geographic location (latitude)

text: 116. 3 E

characteristic: geographic location (longitude)

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: metagenome

text: systemic bacteria in ST-elevation myocardial infarction

characteristic: project name

text: metagenome

text: pyrosequencing

characteristic: sequencing method

text: blood plasma


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:ommonwealth Science and Industrial Research Organisation - Australia

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2�m filters from ~4L of water, from a depth of 28 m within the sinkhole (1 of 2 replicates).

characteristic: description

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: Metagenomic investigation of six depths from the anchialine Bundera sinkhole

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 28 m

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ommonwealth Science and Industrial Research Organisation - Australia

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 28 m within the sinkhole (2 of 2 replicates).

text: aquatic

characteristic: environment (biome)

text: coastal water body

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Australia

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 28 m

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 18 m within the sinkhole (1 of 2 replicates).

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: Bundera sinkhole - 18 m

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

text: public

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 18 m within the sinkhole (2 of 2 replicates).

characteristic: description

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 18 m

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 17 m within the sinkhole (1 of 2 replicates).

text: aquatic

characteristic: environment (biome)

text: coastal water body

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Australia

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 17 m

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 17 m within the sinkhole (2 of 2 replicates).

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: Bundera sinkhole - 17 m

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 22 m within the sinkhole (1 of 2 replicates).

characteristic: description

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 22 m

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 22 m within the sinkhole (2 of 2 replicates).

text: aquatic

characteristic: environment (biome)

text: coastal water body

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Australia

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 22 m

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 8 m within the sinkhole (1 of 2 replicates).

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: Bundera sinkhole - 8 m

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

text: public

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 8 m within the sinkhole (2 of 2 replicates).

characteristic: description

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 8 m

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 2 m within the sinkhole (1 of 2 replicates).

text: aquatic

characteristic: environment (biome)

text: coastal water body

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Australia

text: metagenome

text: metagenome

text: metagenome

text: Illumina HiSeq

text: Bundera sinkhole - 2 m

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Bundera sinkhole is an anchialine environment in Western Australia. Microbial biomass was collected on 0.2µm filters from ~4L of water, from a depth of 2 m within the sinkhole (2 of 2 replicates).

text: aquatic

text: coastal water body

text: water

text: Australia

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: Bundera sinkhole - 2 m

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BERN

characteristic: INSDC first public

text: public

tag: Namespace:UNIVERSITY OF BERN

text: human

characteristic: common name

text: SMC untreated

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Male

characteristic: sex

text: Bladder smooth muscle

characteristic: tissue type

text: Untreated bladder smooth muscle cells

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BERN

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: SMC treated 24h

text: Homo sapiens

text: Homo sapiens

text: Male

text: Bladder smooth muscle

text: TNF-treated bladder smooth muscle cells


text: UNIVERSITY OF BERN

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: human

text: SMC treated 48h

text: Homo sapiens

text: Homo sapiens

text: Male

text: Bladder smooth muscle

text: TNF-treated bladder smooth muscle cells


text: Vibrio cholerae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Vibrio cholerae

text: Vibrio cholerae

text: Vibrio cholerae

characteristic: subject id


text: Staphylococcus aureus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Streptococcus agalactiae

text: MRI Z2-310

text: SC

characteristic: INSDC first public

text: public

text: Streptococcus agalactiae

characteristic: common name

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: Streptococcus agalactiae

text: AMR 1586

text: SC

text: public

text: Streptococcus agalactiae

text: Streptococcus agalactiae

characteristic: organism

text: Streptococcus agalactiae


text: E. coli?

text: SC

text: public

text: E. coli?

text: Escherichia coli

text: Escherichia coli


text: Klebsiella pneumoniae

text: SC

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: ED

text: public

text: somatic

characteristic: cell type

text: black field cricket

text: gDNA reads - Illumina, TruSeq 180 bp library

characteristic: description

text: adult

characteristic: dev stage

text: Teleogryllus oceanicus

text: Teleogryllus oceanicus

text: LibA


characteristic: ENA-CHECKLIST

text: ED

text: public

text: somatic

text: black field cricket

text: gDNA reads - Illumina, TruSeq Nano 350 bp library

text: adult

text: Teleogryllus oceanicus

text: Teleogryllus oceanicus

text: LibD

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ED

characteristic: INSDC last update

text: public

text: somatic, thoracic

text: black field cricket

text: RNAseq reads, R1 and R2

text: embryo

text: Teleogryllus oceanicus

text: Teleogryllus oceanicus


text: INSTITUTE OF EVOLUTIONARY BIOLOGY

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Unconverted

text: Daphnia magna

text: Daphnia magna

text: Genome-wide Methylation Patterns Under Caloric Restriction in Daphnia magna


text: INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Converted

text: Daphnia magna

text: Daphnia magna


text: University of California, San Diego

text: public

text: E-MTAB-6466:BMDM_ChIP_H3K36me3_0hKLA.fastq.gz

tag: Namespace:University of California, San Diego

text: 8 to 10

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

text: house mouse

text: Protocols: Human cells were cultured and collected by following a standard protocol. For mouse bone marrow-derived macrophages (BMDM), bone marrow was isolated from 8- to-10-week-old C57Bl/6 (Harlan Laboratories) female mice. BMDM were generated in DMEM supplemented with M-CSF for 7 days and activated with Kdo2-Lipid A (KLA) for 6 hours as described in Kaikkonen, M. U. et al. Remodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription. Mol. Cell 51, 310-325. Antibodies against H3K27me3 (07-449) were from Millipore Human Cells were maintained in DMEM with 10% FBS, 5% CO2, 37oC. Mouse BMDMs were generated from bone marrow and cultured in DMEM supplemented with M-CSF for 7 days and activated with KLA as descried. Mouse BMDM were treated with 100 ng/ml of Kdo2-Lipid A as indicated. For human ChIP-seq, ~15 million wild-type HeLa S3 cells or the indicated hNDF (NRG1) knockout cells were collected and crosslinked with 1% formaldehyde. After cell lysis and genome fragmentation by sonication, samples were incubated with 20 μL of Dynabeads Protein A that were pre-bound with 1 μL of NDF antiserum. [Note that 1 μL of hNDF antiserum was optimal for hNDF antiserum #7. For hNDF antiserum #6, 2 μL was the optimal volume.] Bound DNA were isolated with MinElute columns (Qiagen). For human RNA-seq, total RNA was purified by using the PureLink RNA Mini RNA Kit (Thermo Fisher Invitrogen). Mouse BMDM ChIP-seq and RNA-seq were performed as described in Kaikkonen, M. U. et al. Remodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription. Mol. Cell 51, 310-325. Human ChIP-seq libraries were prepared by end-repair, A-tailing, and ligation of Illumina TruSeq adaptors, which was then followed by PCR amplification and size selection with AMPure beads (Beckman Coulter). Human RNA-seq libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). Mouse ChIP-seq libraries were prepared from ChIP DNA and inputs by blunting, A-tailing, and adapter ligations were performed by using NextFlex barcode adapters. Mouse RNA-seq libraries were prepared from poly(A)-enriched mRNA, as described in Kaikkonen, M. U. et al. Mol. Cell 51, 310-325.

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of California, San Diego

characteristic: INSDC first public

text: public

text: E-MTAB-6466:BMDM_ChIP_H3K36me3_6hKLA.fastq.gz

text: 8 to 10

text: ArrayExpress

text: bone marrow macrophage

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of California, San Diego

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-6466:BMDM_ChIP_NDF7_0hKLA.fastq.gz

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: title


text: University of California, San Diego

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-6466:BMDM_ChIP_NDF7_6hKLA.fastq.gz

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

text: house mouse

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of California, San Diego

characteristic: INSDC first public

text: public

text: E-MTAB-6466:BMDM_RNA_0hKLA_rep1.fastq.gz

text: 8 to 10

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of California, San Diego

text: public

text: E-MTAB-6466:BMDM_RNA_0hKLA_rep2.fastq.gz

text: 8 to 10

text: ArrayExpress

text: bone marrow macrophage

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female


text: University of California, San Diego

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6466:BMDM_RNA_6hKLA_rep1.fastq.gz

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of California, San Diego

text: public

text: E-MTAB-6466:BMDM_RNA_6hKLA_rep2.fastq.gz

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

text: house mouse

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of California, San Diego

characteristic: INSDC first public

text: public

text: ArrayExpress

text: HeLa S3

characteristic: cell line

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens


text: University of California, San Diego

characteristic: INSDC center name

text: public

text: ArrayExpress

text: HeLa S3

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Human cells were cultured and collected by following a standard protocol. For mouse bone marrow-derived macrophages (BMDM), bone marrow was isolated from 8- to-10-week-old C57Bl/6 (Harlan Laboratories) female mice. BMDM were generated in DMEM supplemented with M-CSF for 7 days and activated with Kdo2-Lipid A (KLA) for 6 hours as described in Kaikkonen, M. U. et al. Remodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription. Mol. Cell 51, 310-325. Antibodies against H3K27me3 (07-449) were from Millipore Human Cells were maintained in DMEM with 10% FBS, 5% CO2, 37oC. Mouse BMDMs were generated from bone marrow and cultured in DMEM supplemented with M-CSF for 7 days and activated with KLA as descried. For human ChIP-seq, ~15 million wild-type HeLa S3 cells or the indicated hNDF (NRG1) knockout cells were collected and crosslinked with 1% formaldehyde. After cell lysis and genome fragmentation by sonication, samples were incubated with 20 μL of Dynabeads Protein A that were pre-bound with 1 μL of NDF antiserum. [Note that 1 μL of hNDF antiserum was optimal for hNDF antiserum #7. For hNDF antiserum #6, 2 μL was the optimal volume.] Bound DNA were isolated with MinElute columns (Qiagen). For human RNA-seq, total RNA was purified by using the PureLink RNA Mini RNA Kit (Thermo Fisher Invitrogen). Mouse BMDM ChIP-seq and RNA-seq were performed as described in Kaikkonen, M. U. et al. Remodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription. Mol. Cell 51, 310-325. Human ChIP-seq libraries were prepared by end-repair, A-tailing, and ligation of Illumina TruSeq adaptors, which was then followed by PCR amplification and size selection with AMPure beads (Beckman Coulter). Human RNA-seq libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). Mouse ChIP-seq libraries were prepared from ChIP DNA and inputs by blunting, A-tailing, and adapter ligations were performed by using NextFlex barcode adapters. Mouse RNA-seq libraries were prepared from poly(A)-enriched mRNA, as described in Kaikkonen, M. U. et al. Mol. Cell 51, 310-325.

characteristic: description

text: cervical adenocarcinoma

text: NDF homozygous knockout

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens


text: University of California, San Diego

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: HeLa S3

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

text: Homo sapiens

characteristic: title


text: Brown Trout

characteristic: ArrayExpress-SPECIES

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: brown trout

text: Salmo trutta

text: Salmo trutta


text: Human Gut Microbiota

characteristic: ArrayExpress-PHENOTYPE

text: gut metagenome

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: gut metagenome

text: gut metagenome

text: Human Gut Microbiota

characteristic: phenotype

text: gut metagenome

characteristic: subject id


text: female

characteristic: ArrayExpress-SEX

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: house mouse

text: Female

characteristic: gender

text: Mus musculus

text: Mus musculus


Cheese B

text: CERELA

characteristic: INSDC last update

text: public

text: Cheese B

text: Nadia Suarez, Ivana Slozilová, Lucila Saavedra, Fernando Sesma.

characteristic: collected by

characteristic: collection date

text: The ion semiconductor technology using an IonTorrent PGM system, was used as a metagenomic based approach to assess the taxonomic affiliation and function potential for bacteriocin production of the bacterial populations in cow’s milk artisanal cheeses from the northwest Argentina.

characteristic: description

text: Yes

characteristic: environmental sample

text: Argentina

characteristic: geographic location (country and/or sea)

text: Tucumán

characteristic: geographic location (region and locality)

text: semi-hard cow’s milk artisanal cheeses

characteristic: isolation source

text: metagenome

characteristic: organism

text: metagenome

text: Metagenome Cheese B


E-MTAB-6469:BRB-seq V1

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6469:BRB-seq V1

tag: Namespace:Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland

characteristic: Submitter Id

text: T0A (GTCAAG), T0B (ATGTAA), T14A (GACGGG), T14B (CCACGC)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

text: adipose tissue derived mesenchymal stem cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: hASC were obtained from a fresh lipoaspirate as following. 50 ml of lipoaspirate was washed twice with 40 ml of DPBS Ca+/Mg+ (Gibco, #14040091) in 100 ml syringes and incubated with 0.28 U/ml of liberase TM (Roche, #05401119001(ROC)) for 45 min at 37ºC under agitation. The digested tissue was mixed with 40 ml of CRB (1% human albumin (CSL Behring) in 40 ml of DPBS -/- (Gibco, #14190094)) and shaken vigorously to liberate the stromal cells. The aqueous phase was recovered and centrifuged at 400g for 5 minutes at RT. The cell pellet was resuspended in 15 ml of CRB and filtered through a 100 µm and then 40 µm cell strainer to ensure a single cell preparation, centrifuged and resuspended in Minimum Essential Medium (MEM) alpha (Gibco, #32561037) supplemented with 5% human platelet lysate (Cook Regentec, #G34936) and 50 µg/mL Primocin (InvivoGen, #ant-pm-1). hASCs were cultured in the same media composition until 70-80% confluency and detached using TrypLE Select (Life Technology, 1256311) for passaging. For the adipogenic differentiation, cells at confluence were treated with induction cocktail from Adipogenic BulletKit (Lonza, PT-3004) for 7 days, followed by treatment with maintenance cocktail for another 7 days. Total RNA was isolated using TRIzol (Sigma) and followed by double precipitation with ethanol. BRB-seq novel protocol (see referenced paper - https://www.biorxiv.org/content/biorxiv/early/2018/01/30/256594.full.pdf)

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 100 ng

characteristic: rna per sample

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

text: BRB-seq V1

characteristic: title

text: B/B

text: BRB-seq v1

characteristic: used for


E-MTAB-6469:BRB-seq V1 (rep)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6469:BRB-seq V1 (rep)

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: technical replicate

characteristic: replicate

text: 100 ng

text: MMH

text: Homo sapiens

text: PCR

text: BRB-seq V1 (rep)

text: B/B

text: BRB-seq v1 (replicate)


name: E-MTAB-6469:Preamplification 5 cycles (1000ng RNA)

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: T0A (AGGCGG), T0B (CACCGG), T14A (CAGCGC), T14B (CCACGC)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

characteristic: cell type

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 1000 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 5 cycles (1000ng RNA)

text: B/B

text: Amplification & Initial RNA effect (Fig 2D & Supp Fig S1A)


name: E-MTAB-6469:Preamplification 5 cycles (100ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: T0A (TCAAAA), T0B (TCTAAT), T14A (TGAATA), T14B (TGTATT)

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: 100 ng

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

text: Preamplification 5 cycles (100ng RNA)

characteristic: title

text: B/B

characteristic: used for


name: E-MTAB-6469:Preamplification 5 cycles (2000ng RNA)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T0A (CGCGTG), T0B (CGGAGC), T14A (CGGCTG), T14B (CGGGTC)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 2000 ng

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 5 cycles (2000ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 5 cycles (500ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: T0A (ATCGAG), T0B (CATCAG), T14A (CGACTT), T14B (GAAGAC)

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 500 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 5 cycles (500ng RNA)

text: B/B


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: 100 ng

text: SSII

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

characteristic: title

text: B/B

text: Supp Figure S2 (PCR_Sup)

characteristic: used for


E-MTAB-6469:SSII_PCR (rep)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6469:SSII_PCR (rep)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: technical replicate

characteristic: replicate

text: 100 ng

text: SSII

text: Homo sapiens

text: PCR

text: SSII_PCR (rep)

text: B/B

text: Supp Figure S2 (PCR_Sup, replicate)


name: E-MTAB-6469:Preamplification 10 cycles (1000ng RNA)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 1000 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 10 cycles (1000ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 10 cycles (100ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: 100 ng

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

text: Preamplification 10 cycles (100ng RNA)

characteristic: title

text: B/B

characteristic: used for


name: E-MTAB-6469:Preamplification 10 cycles (10ng RNA)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T0A (CTTAAT), T0B (GAATAT), T14A (GATTAA), T14B (GTTAAA)

text: ArrayExpress

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 10 ng

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 10 cycles (10ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 10 cycles (500ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: barcodes

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 500 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 10 cycles (500ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 15 cycles (1000ng RNA)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: 1000 ng

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

text: Preamplification 15 cycles (1000ng RNA)

characteristic: title

text: B/B

characteristic: used for


name: E-MTAB-6469:Preamplification 15 cycles (100ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 100 ng

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 15 cycles (100ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 15 cycles (10ng RNA)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: barcodes

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

characteristic: pre-amplification

text: 10 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 15 cycles (10ng RNA)

text: B/B


name: E-MTAB-6469:Preamplification 15 cycles (1ng RNA)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: T0A (AGAATT), T0B (AGTATA), T14A (ATAAGT), T14B (ATAGAT)

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: 1 ng

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: PCR

characteristic: second-strand synthesis

text: Preamplification 15 cycles (1ng RNA)

characteristic: title

text: B/B

characteristic: used for


name: E-MTAB-6469:Preamplification 15 cycles (500ng RNA)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 500 ng

text: MMH

text: Homo sapiens

text: PCR

text: Preamplification 15 cycles (500ng RNA)

text: B/B


E-MTAB-6469:BRB-seq V2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6469:BRB-seq V2

characteristic: barcodes

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

text: 100 ng

characteristic: rna per sample

text: SSII

text: Homo sapiens

text: Nick translation

text: BRB-seq V2

text: B/B

text: BRB-seq v2


E-MTAB-6469:BRB-seq V2 (degraded)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6469:BRB-seq V2 (degraded)

text: T0_nt (GGACAT), T14_nt (GTCAAG), T0_1mn (TCCAAC), T14_1mn (TTGTCC), T0_2mn (CTCTCC), T14_2mn (ACCCGG)

text: ArrayExpress

characteristic: broker name

text: human

text: [T14_nt, T0_2min] Low read number (<1M)

characteristic: note

text: Homo sapiens

text: Homo sapiens

text: technical replicate

characteristic: replicate

text: 100 ng

text: SSII

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: Nick translation

characteristic: second-strand synthesis

text: BRB-seq V2 (degraded)

characteristic: title

text: B/B

text: Degradation (T0_nt used as replicate for Fig 4 B)

characteristic: used for


E-MTAB-6469:BRB-seq V2 (degraded, 1mn)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6469:BRB-seq V2 (degraded, 1mn)

characteristic: Submitter Id

text: ArrayExpress

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 100 ng

text: SSII

text: Homo sapiens

text: Nick translation

text: BRB-seq V2 (degraded, 1mn)

text: B/B

text: Degradation (Fig 4 A&B)


E-MTAB-6469:BRB-seq V2 (degraded, 2mn)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6469:BRB-seq V2 (degraded, 2mn)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

text: 100 ng

characteristic: rna per sample

text: SSII

text: Homo sapiens

text: Nick translation

text: BRB-seq V2 (degraded, 2mn)

text: B/B

text: Degradation (Fig 4 A&B)


E-MTAB-6469:BRB-seq V2 (degraded, nt)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6469:BRB-seq V2 (degraded, nt)

text: ArrayExpress

text: human

text: [T0A] Low read number (<1M)

characteristic: note

text: Homo sapiens

text: Homo sapiens

text: 100 ng

text: SSII

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: Nick translation

characteristic: second-strand synthesis

text: BRB-seq V2 (degraded, nt)

characteristic: title

text: B/B

text: Degradation (Fig 4 A&B)

characteristic: used for


E-MTAB-6469:BRB-seq V2 (rep)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6469:BRB-seq V2 (rep)

characteristic: Submitter Id

text: ArrayExpress

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: technical replicate

characteristic: replicate

text: 100 ng

text: SSII

text: Homo sapiens

text: Nick translation

text: BRB-seq V2 (rep)

text: B/B

text: BRB-seq v2 (replicate)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: [T0A, T0B] Low read number (<1M)

characteristic: note

text: Homo sapiens

text: Homo sapiens

text: 100 ng

characteristic: rna per sample

text: MMH

text: Homo sapiens

text: Nick translation

text: B/B

text: Supp Figure S2 (SSS_Max)


E-MTAB-6469:MMH_Nick (rep)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6469:MMH_Nick (rep)

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: technical replicate

text: 100 ng

text: MMH

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: Nick translation

characteristic: second-strand synthesis

text: MMH_Nick (rep)

characteristic: title

text: B/B

text: Supp Figure S2 (SSS_Max, replicate)

characteristic: used for


name: E-MTAB-6469:Second Amplification (10 cycles)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: T0A (AAGTTA), T0B (ATTATC), T14A (TACAAA), T14B (TTCATA)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 1000 ng

text: SSII

text: Homo sapiens

text: Nick translation

text: Second Amplification (10 cycles)

text: B/B

text: Second amplification effect (Supp Fig S1C)


name: E-MTAB-6469:Second Amplification (12 cycles)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: human

characteristic: common name

characteristic: nb multiplexed samples

characteristic: nb reads

text: Homo sapiens

text: Homo sapiens

text: 1000 ng

characteristic: rna per sample

text: SSII

text: Homo sapiens

text: Nick translation

text: Second Amplification (12 cycles)

text: B/B


name: E-MTAB-6469:Second Amplification (8 cycles)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: Submitter Id

text: ArrayExpress

text: human

text: [All] Low read number (<1M / sample)

characteristic: note

text: Homo sapiens

text: Homo sapiens

text: 1000 ng

text: SSII

characteristic: rt enzyme

text: Homo sapiens

characteristic: second amplification

text: Nick translation

characteristic: second-strand synthesis

text: Second Amplification (8 cycles)

characteristic: title

text: B/B

characteristic: used for


E-MTAB-6469:Tn5 (A/B)

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6469:Tn5 (A/B)

characteristic: barcodes

text: ArrayExpress

characteristic: broker name

characteristic: cdna

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: 1000 ng

text: SSII

text: Homo sapiens

text: Nick translation

text: Tn5 (A/B)

text: A/B

text: Tn5 comparison (Supp Fig S1B)