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text: osteoarthritis, knee

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text: Protocols: Cartilage was obtained under Ethics Committee approval with written informed consent from 60 patients with symptomatic knee osteoarthritis (OA). Cartilage from osteochondral samples were isolated from the intact PLC (posterior lateral condyle) for RNA-seq analysis. Comparable PLC samples from 10 non-OA joints were obtained from above knee amputations of patients with peripheral vascular disease and no history or clinical sign of osteoarthritis, joint disease, or joint trauma. Total RNA was extracted from each patient sample of 200 to 400 mg of cartilage using TRIzol (LifeTechnologies) reagent and homogenisation (Braun Mikrodismembrator) following freezing in liquid nitrogen. The RNA was purified using RNeasy Qiagen clean-up columns (Qiagen) and for sequencing had a RIN score of >6 (2200 TapeStation, Agilent Technologies). Strand specific RNA-seq libraries were generated from 0.5-1 ug RNA using the TruSeq Stranded mRNA assay (Illumina, Inc.) using the manufacturers Stranded mRNA Sample Preparation Kit and 101bp paired-end reads were generated. Total RNA was extracted from 200-400 mg of cartilage using TRIzol (Life-technologies) reagent and homogenisation (Braun Mikrodismembrator) following freezing in liquid nitrogen. The RNA was purified using RNeasy Qiagen clean-up columns (Qiagen) and for sequencing had a RIN score of >6 (2200 TapeStation, Agilent Technologies).

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BLANK

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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest.

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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest.


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tag: Namespace:National Institute of Public Health - National Institute of Hygiene

text: NIPH-NIH

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

text: Poland

characteristic: geo loc name

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

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characteristic: isolation source host associated

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text: Salmonella enterica

characteristic: serovar

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text: Poland

characteristic: geo loc name

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

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text: Homo sapiens

text: Yes

text: feaces

text: Salmonella enterica

text: Salmonella enterica

text: Senftenberg


characteristic: ENA-CHECKLIST

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text: NIPH-NIH

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text: Poland

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characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: rectal swab

characteristic: isolation source host associated

text: Salmonella enterica

text: Salmonella enterica

characteristic: serovar

characteristic: strain