text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232374-sc-2011-10-04T11:18:39Z-1284653

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


status: PUBLIC

text: Xenopus tropicalis

tag: attribute

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC_XTEP5232388-sc-2011-10-04T11:18:51Z-1284667

characteristic: Submitter Id

text: western clawed frog

characteristic: common name

text: Xenopus (Silurana) tropicalis

characteristic: organism

text: toe clip DNA prep

characteristic: sample description

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232344-sc-2011-10-04T11:18:11Z-1284623

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232385-sc-2011-10-04T11:18:48Z-1284664

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232323-sc-2011-10-04T11:17:52Z-1284602

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232393-sc-2011-10-04T11:18:55Z-1284672

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232373-sc-2011-10-04T11:18:37Z-1284652

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232392-sc-2011-10-04T11:18:54Z-1284671

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232387-sc-2011-10-04T11:18:50Z-1284666

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232346-sc-2011-10-04T11:18:13Z-1284625

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232382-sc-2011-10-04T11:18:46Z-1284661

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232377-sc-2011-10-04T11:18:42Z-1284656

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232359-sc-2011-10-04T11:18:26Z-1284638

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232375-sc-2011-10-04T11:18:40Z-1284654

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232398-sc-2011-10-04T11:18:59Z-1284677

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232402-sc-2011-10-04T11:19:02Z-1284681

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232322-sc-2011-10-04T11:17:49Z-1284601

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232386-sc-2011-10-04T11:18:49Z-1284665

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Mouse

text: SC

text: public

text: 1912STDY5200837-sc-2011-08-08T12:50:20Z-1247713

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: 1912STDY5200836-sc-2011-08-08T12:50:20Z-1247712

text: house mouse

text: Mus musculus

text: Mus musculus


text: MWG Operon, Germany

text: public

tag: Namespace:MWG Operon, Germany

text: ArrayExpress

characteristic: broker name

text: platelet

characteristic: cell type

text: human

text: Protocols: The local Regional Ethical Review Board (Link_ping, Sweden) granted an ethical permission for this study. Unstimulated non-irradiated apheresis platelets were collected from a healthy human male blood donor. Platelets were collected by COBE Spectra system (Gambro BCT) and were used on the same collection day. Residual leukocytes were depleted with anti-CD45 conjugated Dynabeads, according to the manufacturers recommendations (Pan Leukocyte; Invitrogen, Carlsbad, CA). The platelet suspension, with a volume of 30 mL and a platelet count of 1.4 * 10exp9 cells/mL, was centrifuged at 800 g for 8 min and the supernatant was discarded. The platelet pellet was processed for leukocyte depletion, as recommended by the supplier of Pan Leukocyte reagent (Invitrogen, Carlsbad, CA). The leukocyte removal was performed at room temperature. Approximately 70 _ 75% of the original platelets were recovered after the leukocyte depletion. To investigate the level of leukocyte contamination, we determined the level of CD45 (PTPRC) transcript by qPCR using TaqMan Gene Expression Assay for this gene according to the recommendations of the supplier (Assay ID: Hs00894713_m1; Applied Bioystems, Carlsbad, CA, USA). Extraction of mRNA and synthesis of cDNA were performed by the method described by Rox JM, Bugert P, Muller J, Schorr A, Hanfland P, et al. (2004) [Gene expression analysis in platelets from a single donor: evaluation of a PCR-based amplification technique. Clin Chem 50: 2271-2278] with the exception that we used Smarter PCR cDNA Synthesis kit for the cDNA synthesis (Clontech, Mountain View, CA, USA). Briefly, the leukocyte-depleted platelet suspension was centrifuged at 1000g for 10 min and the supernatant was discarded. Poly-A mRNA was isolated from the platelet cell pellet by using Dynabeads Oligo(dT)25 according to the instruction of the manufacturer (Invitrogen, Carlsbad, CA, USA). To synthesize the first-strand cDNA, 3.5 uL of poly-A mRNA was combined with 1uL of 3I-Smart CDS primer II A (12 uM). After mixing the tube, the sample was incubated at 72C for 3 min and then at 42C for 2 min. This was followed by the addition of a master mix containing 2 uL of 5_ First-Strand Buffer, 0.25 uL DTT (100 mM), 1 uL dNTP mix (10 mM), 1 uL SMARTer IIA oligonucleotide (12 uM), 0.25 uL RNase inhibitor, and 1 uL of SMARTScribe Reverse Transcriptase (100 U). The reverse transcription was run by incubating the tube at 42C for 1 h before the reaction was terminated at 70C for 10 min. The sample was then diluted with 90 uL of TE-buffer (10 nM Tris, 1 nM EDTA, pH 8.0). To run Long-Distance PCR, 10 uL of the diluted and reverse-transcribed platelet cDNA was mixed with 10 uL 10_ Advantage 2 PCR buffer, 2 uL 50_ dNTP mix (10 mM), 2 uL 5IPCR primer IIA (12 uM), 2 uL 50_ Advantage 2 polymerase mix, and 74 uL of deionised water to a final volume of 100 uL. The sample was then incubated in a thermal cycler running a PCR program containing 95C for 1 min, and then 20 cycles of 95C for 15 s, 65C for 30 s, and 68C for 3 min. The synthesized platelet cDNA was purified with QIAquick PCR purification kit according to the manufacturerIs instructions (Qiagen, Hilden, Germany), and the amount of cDNA was estimated on a Nanodrop spectrophotometer (ND1000; Saveen & Werner, Limhamn, Sweden).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease state

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

characteristic: sex

characteristic: title

type: has member


characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

characteristic: INSDC center name

text: public

text: m_SpermLowNaCl_CXXC_M-sc-2010-03-05-7578

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: m_BloodLowNaCl_CXXC_M-sc-2010-03-05-7580

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_BloodLowNaCl_CXXC_M-sc-2010-03-05-7581

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


text: SC

text: public

text: m_CerebellumLowNaCl_CXXC_M-sc-2010-03-05-7583

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_ESCFP1minLowNaClCXXC2_M-sc-2011-01-27-44821

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_p_m_highNaCl_CXXC_M-sc-2010-03-05-7577

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_NullEV2_Pol2N20_M-sc-2010-11-17-1009289

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_ESnullDS1_CFP1Skal_M-sc-2011-02-24-33262

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_C169A_H3K4me3_151110_M-sc-2010-11-17-1009287

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_ESnullC169Arescue_H3K4me3_M-sc-2011-02-24-33264

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_C169A_Pol2_4H8_M-sc-2010-11-17-1009295

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_NullEV2_H3K4me1_M-sc-2010-11-17-1009297

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

characteristic: ArrayExpress-Sex

text: mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: m_WTrescue_Cfp1DS-sc-2011-03-30T16:43:43Z-1114641

text: house mouse

text: Mus musculus

text: Cfp1 ChIP in mouse Cfp1 Null ES cells + WT rescue (clone 1), library

characteristic: sample description

text: Mus musculus


text: SC

text: public

text: m_ESnullDS1_H3K27me3_M-sc-2011-02-24-33271

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_WTrescue_Pol2N20_M-sc-2010-11-17-1009290

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_NullEV2_H3K4me3_151110_M-sc-2010-11-17-1009285

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_ESnullEV2_H3K4me3_M-sc-2011-02-24-33263

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_WTrescue_H3K4me3_151110_M-sc-2010-11-17-1009286

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_WTrescue__Pol2_4H8_M-sc-2010-11-17-1009294

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_NullEV2_H2B_Ubi_M-sc-2010-11-17-1009305

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_wtES_H3K4me3_151110_M-sc-2010-11-17-1009284

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_NullEV2_Pol2_4H8_M-sc-2010-11-17-1009293

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

text: mus musculus

text: SC

text: public

text: m_C169A_Cfp1DS-sc-2011-03-30T16:43:43Z-1114642

text: house mouse

text: Mus musculus

text: Cfp1 ChIP in mouse Cfp1 Null ES cells + C169A rescue, library

text: Mus musculus


text: SC

text: public

text: m_WTrescue_Input_M-sc-2010-11-17-1009302

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_WTrescue_H2B_Ubi_M-sc-2010-11-17-1009306

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_ESnullWT1rescue_H3K4me3_M-sc-2011-02-24-33270

text: house mouse

text: Mus musculus

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_KO_kainate_NRO_M-sc-2011-04-13T15:18:24Z-1122731

text: house mouse

text: Mus musculus

text: Library PCR

text: Mus musculus


text: SC

text: public

text: m_wt3T3_CFP1Skalnik_M-sc-2011-02-24-985955

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_kd3T3_CFP1Skalnik_M-sc-2011-02-24-985956

text: house mouse

text: Mus musculus

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_KO_saline_NRO_M-sc-2011-04-13T15:18:23Z-1122730

text: house mouse

text: Mus musculus

text: Library PCR

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_wt_saline_NRO_M-sc-2011-04-13T15:18:22Z-1122728

text: house mouse

text: Mus musculus

text: Library PCR

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_wt_kainate_NRO_M-sc-2011-04-13T15:18:23Z-1122729

text: house mouse

text: Mus musculus

text: Library PCR

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_Glial_GFP_repeat_M-sc-2011-04-27T16:46:34Z-1127970

text: house mouse

text: Mus musculus

text: Mus musculus


text: Anti RNA Polymerase II. Abcam 8WG16

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

characteristic: ArrayExpress-OrganismPart

text: Mus musculus

text: SC

text: public

text: m_Brain_PolII_M-sc-2012-06-20T14:29:51Z-6681

text: house mouse

text: Mus musculus

text: DNA isolated from ChIP

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_Neurons_GFP_repeat_M-sc-2011-04-27T16:47:23Z-1127971

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_BrainMeCp2__PolII_M-sc-2010-03-05-6682

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: mus musculus

text: SC

text: public

text: m_Input_GFP_repeat_M-sc-2011-04-27T16:47:57Z-1127972

text: house mouse

text: Mus musculus

text: Mus musculus


text: Yale Center for Genomic Analysis

text: public

tag: Namespace:Yale Center for Genomic Analysis

text: ArrayExpress

characteristic: broker name

text: MCF 10A

characteristic: cell line

text: epithelial

characteristic: cell type

text: human

text: Protocols: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. IMR90 cells were cultured in MEM with Earles salts with 10% FBS. We have followed the ChIP protocol previously published in Kim, T.H. et al. Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome. Cell 128, 17382889 (2007). The ChIP DNA was further processed by the Yale Center for Genomic Analysis following the instructions from Illumina, Inc. 35 bases were sequenced.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Yale Center for Genomic Analysis

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fibroblast

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Yale Center for Genomic Analysis

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fibroblast

text: human

characteristic: common name

text: Protocols: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. IMR90 cells were cultured in MEM with Earles salts with 10% FBS. H1 (from WiCell) embryonic stem cells were cultured on feeder-free matrigel coated plates in Dulbecco's modified Eagle medium (DMEM)/F12, supplemented with 1% MEM-nonessential amino acids (Invitrogen), 1 mM L-glutamine, 1% penicillin-streptomycin, 50 ng/mL basic fibroblast growth factor (Millipore), N2 supplements (1X), and B27 supplements (1X) (Invitrogen). We have followed the protocol previously published in Core, L., Waterfall, J. & Lis, J. Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845-1848 (2008). Briefly, nuclei from treated cells were isolated and incubated for 5 minutes with nucleotides and bromo-UTP analog. The nascent RNA from the labeling reaction was isolated and subjected to immunoaffinity purification using Br-dUTP antibody (Santa Cruz Biotech). After first round of purification of nascent RNA, the resulting RNA was ligated with 5' small RNA adaptor RNA (Illumina) and subjected to second Br-UTP purififcation. The resulting RNA is futher ligated using 3' adaptor RNA and subjected to third round of purification. The final purified RNA was reverse transcribed and PCR amplified and size selected (150-250bp) using polyacrylamide gel electrophoresis to generate the final sequencing library.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Yale Center for Genomic Analysis

text: public

text: ArrayExpress

text: MCF 10A

text: epithelial

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


text: Yale Center for Genomic Analysis

text: public

text: ArrayExpress

text: embryonic cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Yale Center for Genomic Analysis

text: public

text: ArrayExpress

text: MCF 10A

text: epithelial

text: human

text: Protocols: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. MCF10A cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's directions.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


WGP on wheat

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

text: INRA

text: public

text: WGP on wheat

text: bread wheat

text: 16,128 BACs were pooled from 42 384-well plates in a 3D fashion into row, column and splitbox pools resulting in 22 pools per plate i.e a total of 924 pools. Whole Genome Profiling technology was performed on these pools and tags of 36 nt was obtained for each pool.

text: Triticum aestivum

text: Triticum aestivum

text: subset of 16,128 clones of the wheat chromosome 3B BAC library


text: GSC

text: public

text: Perigord truffle

text: Tuber melanosporum

text: Tuber melanosporum

text: Tuber melanosporum


text: Wellcome Trust Sanger Institute

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: frozen sample

characteristic: bio source type

text: Clontech

characteristic: biosourceprovider

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: RNA from tissues was isolated by a modified guanidium thiocynate methods followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. Universal primer and adapter sequences were added to the 5' and 3' ends of cDNA during RACE reaction.

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: Clontech

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male

characteristic: title


text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: C57BL6NJ-sc-2012-08-09T16:36:10Z-1464786

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mus musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: Ryukyu mouse

characteristic: common name

text: Mus caroli

characteristic: organism

text: Mus caroli


text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: SC

text: public

text: dog

text: Canis lupus familiaris

text: Canis lupus familiaris

text: Canine hepatitis virus whole genome sequencing

characteristic: translation missing: en, metadata, sample, sample_metadata, sample_description


text: SC

text: public

text: dog

text: Canis lupus familiaris

text: Canis lupus familiaris

text: Canine parvovirus whole genome sequencing


text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Human hepatitis virus whole geome sequencing


text: LICR-SD

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. BT474, SKBR3 and ZR75-30 were cultured in 10% FBS containing RPMI 1640 at 37C with 5% CO2 When cells are 80% confluent, media was replaced and treated with either 500nM trichostatin A (TSA) or vehicle (DMSO) for 4 hours at 37C. We have followed the protocol previously published in Core, L., Waterfall, J. & Lis, J. Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845-1848 (2008). Briefly, nuclei from treated cells were isolated and incubated for 5 minutes with nucleotides and bromo-UTP analog. The nascent RNA from the labeling reaction was isolated and subjected to immunoaffinity purification using Br-dUTP antibody (Santa Cruz Biotech). After first round of purification of nascent RNA, the resulting RNA was ligated with 5' small RNA adaptor RNA (Illumina) and subjected to second Br-UTP purififcation. The resulting RNA is futher ligated using 3' adaptor RNA and subjected to third round of purification. The final purified RNA was reverse transcribed and PCR amplified and size selected (150-250bp) using polyacrylamide gel electrophoresis to generate the final sequencing library.

text: normal

characteristic: disease state

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: LICR-SD

text: public

text: ArrayExpress

text: human

characteristic: description

text: breast cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

text: GenePool

text: public

text: AsymC

characteristic: StrainOrLine

text: ArrayExpress

text: jewel wasp

text: Protocols: Virgin females were exposed to virgin males and allowed to fully complete all aspects of courtship and copulation. Females were held for a period of 30 minutes or 4-hours post-mating in individual tubes in a 25 degree incubator Female heads and bodies were seperated in RNAlater-ice prior to RNA extraction RNA was extracted from samples using Quiagen's RNAeasy kit

text: Nasonia vitripennis

text: Nasonia vitripennis

text: head

characteristic: organism part

text: Nasonia vitripennis

text: mated

characteristic: state


characteristic: External Id

text: GenePool

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AsymC

text: ArrayExpress

text: jewel wasp

text: Nasonia vitripennis

text: Nasonia vitripennis

text: body

text: Nasonia vitripennis

text: mated


text: GenePool

text: public

characteristic: INSDC status

characteristic: SRA accession

text: AsymC

characteristic: Submitter Id

text: ArrayExpress

text: jewel wasp

characteristic: common name

text: Protocols: Virgin females were used as controls and were held alongside 30 minute mated female samples without exposure to males Female heads and bodies were seperated in RNAlater-ice prior to RNA extraction RNA was extracted from samples using Quiagen's RNAeasy kit

text: Nasonia vitripennis

text: Nasonia vitripennis

text: body

text: Nasonia vitripennis

text: virgin


text: GenePool

text: public

text: AsymC

text: ArrayExpress

characteristic: broker name

text: jewel wasp

text: Nasonia vitripennis

characteristic: organism

text: Nasonia vitripennis

text: head

text: Nasonia vitripennis

text: virgin


characteristic: ENA-FIRST-PUBLIC

text: Babraham Institute sequencing facility

text: public

tag: Namespace:Babraham Institute sequencing facility

text: ArrayExpress

text: baker's yeast

text: Protocols: Total RNA for RNA-Seq was isolated using the hot acidic phenol method (Collart and Oliviero, 2001). Next 10 ug aliquots were treated with DNase I amplification grade (Invitrogen) for 30 minutes at 37C, purified by ethanol precipitation and quality-checked by 8M Urea 6% polyacrylamide gel electrophoresis in 0.5x TBE. PolyA RNA were purified using Oligo-dT Dynabeads (Invitrogen). The dynabeads selectively enrich poly-A RNA. Purified polyA RNA samples were concentrated by ethanol precipitation and then fragmented using the Ambion RNA fragmentation kit. Samples were ethanol precipitated and the RNA was used in first strand and second strand cDNA synthesis with random hexamers at 150 ng/ ul. The entire reaction was used in library generation. Illumina sequencing was performed using protocols derived from (Lefrancois et al., 2009; Marioni et al., 2008; Quail et al., 2009) and the standard Illumina protocol according the manufacturer. DNA fragments were purified after reversal of the crosslink and concentrated using MinElute columns(QIAGEN). Eluted DNAs from two biological replicas (equal amount of DNA) were separated by electrophoresis through 2% agarose in TAE and DNA fragments with size range 150-450 bp were excised. Excised DNA fragment were purified using the QIAGEN Gel Extraction Kit and eluted in 30 ml of EB buffer (10 mM Tris-HCL, pH 8). The entire size selected DNA was then used in the end-filling and A-tailing reactions, essentially as described in the standard Illumina protocol, using standard molecular biology reagents purchased from New England Biolabs. The adapter ligation step was performed using barcoded single-end adapterssynthesized by Sigma-Genosys described in (Lefrancois et al., 2009). Briefly, forward and reverse adapters were mixed in equimolar ratios, incubated at 95C for 5 minutes, and allowed to anneal by using a ramp of -1C/ 10 seconds until the sample reached 4C. The ligation of adapters with DNA fragments was performed using T4 DNA ligase from Enzymatics, with incubation for 30 minute at 16C followed by an additional 30 minutes at 22C. Next the library was purified using the QIAGEN MinElute kit and separated in a 2 % agarose/ TAE gel for 1 hour at 90 V. Libraries were excised from the gel between 150 bp and 500 bp (always superior 110bp to avoid the risk of adapter dimer contamination). Amplification was performed using Pfx Platinum polymerase (Invitrogen) for 15 cycles as described by Quail et al. (Quail et al., 2009). The libraries were concentrated with QIAGEN MinElute kits and electrophoresed in a 1.9 % agarose/ TAE gel. Samples were quantified with SYBR Green qPCR Master Mix (Applied Biosystems) and the primers SYBR FP4 and SYBR RP7 and compared to a standard curve of phiX174 library, described by Quail, Swerdlow, and Turner (2009). The libraries were diluted to 10 nM in EB buffer

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

text: Babraham Institute sequencing facility

text: public

text: ArrayExpress

text: baker's yeast

text: Protocols: Input libraries were created from crosslinked chromatin before chromatin immunoprecipitation. ChIP was carried out essentially as described (Neves-Costa et al., 2009). Briefly, samples were crosslinked 60 min with 1 % final formaldehyde and chromatin extracts were sonicated to 500 bp. Triplicates or duplicate ChIP samples were validated by qPCR. Illumina sequencing was performed using protocols derived from (Lefrancois et al., 2009; Marioni et al., 2008; Quail et al., 2009) and the standard Illumina protocol according the manufacturer. DNA fragments were purified after reversal of the crosslionk and concentrated using MinElute columns (QIAGEN). Eluted DNAs from two biological replicas (equal amount of DNA) were separated by electrophoresis through 2% agarose in TAE and DNA fragments with size range 150-450 bp were excised. Excised DNA fragment were purified using the QIAGEN Gel Extraction Kit and eluted in 30 ml of EB buffer (10 mM Tris-HCL, pH 8). The entire size selected DNA was then used in the end-filling and A-tailing reactions, essentially as described in the standard Illumina protocol, using standard molecular biology reagents purchased from New England Biolabs. The adapter ligation step was performed using barcoded single-end adapters synthesized by Sigma-Genosys described in (Lefrancois et al., 2009). Briefly, forward and reverse adapters were mixed in equimolar ratios, incubated at 95C for 5 minutes, and allowed to anneal by using a ramp of -1C/ 10 seconds until the sample reached 4C. The ligation of adapters with DNA fragments was performed using T4 DNA ligase from Enzymatics, with incubation for 30 minute at 16C followed by an additional 30 minutes at 22C. Next the library was purified using the QIAGEN MinElute kit and separated in a 2 % agarose/ TAE gel for 1 hour at 90 V. Libraries were excised from the gel between 150 bp and 500 bp (always superior 110bp to avoid the risk of adapter dimer contamination). Amplification was performed using Pfx Platinum polymerase (Invitrogen) for 15 cycles as described by Quail et al. (Quail et al., 2009). The libraries were concentrated with QIAGEN MinElute kits and electrophoresed in a 1.9 % agarose/ TAE gel. Samples were quantified with SYBR Green qPCR Master Mix (Applied Biosystems) and the primers SYBR FP4 and SYBR RP7 and compared to a standard curve of phiX174 library, described by Quail, Swerdlow, and Turner (2009). The libraries were diluted to 10 nM in EB buffer.

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


text: Babraham Institute sequencing facility

text: public

characteristic: StrainOrLine

text: ArrayExpress

text: baker's yeast

text: wild type genotype

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: Babraham Institute sequencing facility

text: public

text: ArrayExpress

text: baker's yeast

text: delta fun30

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Babraham Institute sequencing facility

characteristic: INSDC center name

text: public

text: ArrayExpress

text: baker's yeast

text: HTZ1-3myc delta fun30

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Babraham Institute sequencing facility

characteristic: INSDC first public

text: public

text: ArrayExpress

text: baker's yeast

text: delta fun30

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Babraham Institute sequencing facility

characteristic: INSDC last update

text: public

text: ArrayExpress

text: baker's yeast

text: HTA1-3myc delta fun30

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

characteristic: SRA accession

text: 06092208-sc-2011-06-17T10:40:11Z-1165988

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

text: C9_PF13_0027-sc-2011-06-17T10:40:40Z-1165989

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Mus musculus

text: SC

text: public

text: PERI_ZFP-sc-2011-05-12T12:23:44Z-1137500

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: 2E_1_H3K9me3_SP_SOX2CTAP-sc-2011-05-12T12:23:53Z-1137510

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2E_NSC02_SP_SOX2CTAP-sc-2011-05-12T12:23:49Z-1137505

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PERI_ARID-sc-2011-05-12T12:23:46Z-1137501

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2E_H3K4me3_SP_SOX2CTAP-sc-2011-05-12T12:23:49Z-1137506

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: PERI_MTA-sc-2011-05-12T12:23:46Z-1137502

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2E_NSC_RIgG_SP_SOX2CTAP-sc-2011-05-12T12:23:52Z-1137508

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: E14_NSC_SP-sc-2011-05-12T12:23:48Z-1137503

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: Peri_LacZ-sc-2011-05-12T13:42:46Z-1137307

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2E_H3K9me3_SP_SOX2CTAP-sc-2011-05-12T12:23:50Z-1137507

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2E_NSC01_SP_SOX2CTAP-sc-2011-05-12T12:23:48Z-1137504

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: dog

text: Canis lupus familiaris

text: Canis lupus familiaris


text: SC

text: public

text: Tasmanian devil

text: Sarcophilus harrisii

text: Sarcophilus harrisii


text: SC

text: public

text: pig

text: Sus scrofa

text: Sus scrofa


text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: SC

text: public

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax


text: SC

text: public

text: human

text: Homo sapiens

text: short PCR products that have been concatenated and then put through an indexed library prep, before pooling

characteristic: sample description

text: Homo sapiens


text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: IT

characteristic: strain


text: SC

text: public

text: western European house mouse

text: Mus musculus domesticus

text: Mus musculus domesticus


text: B-Lymphocyte

characteristic: ArrayExpress-CellType

text: CTCF

characteristic: ArrayExpress-Immunoprecipitate

text: male

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: CTCF

text: female

text: Homo sapiens

characteristic: External Id

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: male

text: Homo sapiens

text: SC

characteristic: INSDC center name

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: female

text: Homo sapiens

text: SC

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: male

text: Homo sapiens

text: SC

characteristic: INSDC last update

text: public

text: GM12891_rep1-sc-2011-06-09T13:38:46Z-1162268

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: male

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: GM12891_rep4-sc-2011-06-09T13:38:47Z-1162271

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: female

text: Homo sapiens

text: SC

text: public

characteristic: INSDC status

text: GM12892_rep3-sc-2011-06-09T13:38:50Z-1162275

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: female

text: Homo sapiens

text: SC

text: public

characteristic: SRA accession

text: GM12892_rep5-sc-2011-06-09T13:38:51Z-1162277

text: human

text: Homo sapiens

text: Homo sapiens


text: B-Lymphocyte

text: female

text: Homo sapiens

text: SC

text: public

text: GM12892_rep4-sc-2011-06-09T13:38:50Z-1162276

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193730-sc-2011-07-19T14:46:52Z-1240425

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193732-sc-2011-07-19T14:46:54Z-1240427

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: female

text: Homo Sapien

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: GIH5193733-sc-2011-07-19T14:46:56Z-1240428

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193735-sc-2011-07-19T14:46:57Z-1240430

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193731-sc-2011-07-19T14:46:53Z-1240426

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193736-sc-2011-07-19T14:46:57Z-1240431

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193734-sc-2011-07-19T14:46:56Z-1240429

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193737-sc-2011-07-19T14:46:58Z-1240432

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193738-sc-2011-07-19T14:46:58Z-1240433

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193739-sc-2011-07-19T14:46:59Z-1240434

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193741-sc-2011-07-19T14:47:02Z-1240436

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193743-sc-2011-07-19T14:47:03Z-1240438

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193740-sc-2011-07-19T14:46:59Z-1240435

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193744-sc-2011-07-19T14:47:03Z-1240439

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193747-sc-2011-07-19T14:47:05Z-1240442

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193746-sc-2011-07-19T14:47:04Z-1240441

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193745-sc-2011-07-19T14:47:04Z-1240440

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193749-sc-2011-07-19T14:47:08Z-1240444

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193748-sc-2011-07-19T14:47:07Z-1240443

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193751-sc-2011-07-19T14:47:09Z-1240446

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193753-sc-2011-07-19T14:47:10Z-1240448

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193755-sc-2011-07-19T14:47:11Z-1240450

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193754-sc-2011-07-19T14:47:11Z-1240449

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: GIH5193756-sc-2011-07-19T14:47:13Z-1240451

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193757-sc-2011-07-19T14:47:14Z-1240452

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193538-sc-2011-07-19T15:08:01Z-1240233

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: GIH5193758-sc-2011-07-19T14:47:14Z-1240453

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: ACB5193539-sc-2011-07-19T15:08:02Z-1240234

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193540-sc-2011-07-19T15:08:02Z-1240235

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193541-sc-2011-07-19T15:08:04Z-1240236

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193542-sc-2011-07-19T15:08:04Z-1240237

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193544-sc-2011-07-19T15:08:05Z-1240239

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: ACB5193543-sc-2011-07-19T15:08:05Z-1240238

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: ACB5193545-sc-2011-07-19T15:08:06Z-1240240

text: human

text: Homo sapiens

text: Homo sapiens


text: Organism: Macaca mulatta

characteristic: Experimental Factor: ORGANISM

text: OrganismPart: liver

characteristic: Experimental Factor: ORGANISMPART

text: CRUK-CRI

text: public

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

text: adult

characteristic: developmental stage

characteristic: individual

text: Macaca mulatta

text: Macaca mulatta

text: liver

characteristic: organism part

text: Macaca mulatta

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: Organism: Monodelphis domestica

text: OrganismPart: liver

characteristic: External Id

text: CRUK-CRI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: gray short-tailed opossum

text: adult

text: Monodelphis domestica

text: Monodelphis domestica

text: liver

text: Monodelphis domestica

text: male


Runx1 induced

text: BCCS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Runx1 induced

text: house mouse

text: Mouse ErythroLeukaemic (MEL) cell lines were maintained in DMEM supplemented with 10% fetal calf serum and penicillin/streptomycin. MEL cells in the log phase of growth were induced to differentiate with 2% dimethylsulfoxide (DMSO) for four days. V5-Bio or V5-Flag-Bio tagged as described in Optimal use of tandem biotin and V5 tags in ChIP assays, BMC Kolodziej et al. Mol Biol 2009 and A systems approach to analyze transcription factors in mammalian cells, Soler et al. Methods in press 2009.

characteristic: description

text: Mus musculus

text: Mus musculus


characteristic: CEPH-HGDP sample ID

text: MPI-EVA

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Human CEPH-HGDP individual HGDP00980

text: Central African Republic

characteristic: geographic location (country)

text: Homo sapiens

text: Biaka Pygmies

characteristic: population

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: MPI-EVA

text: public

text: human

characteristic: common name

text: Human CEPH-HGDP individual HGDP00679

text: Israel

text: Homo sapiens

characteristic: organism

text: Palestinian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01196

text: China

text: Homo sapiens

text: Miaozu

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00783

text: China

text: Homo sapiens

text: Han

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01098

text: China

text: Homo sapiens

text: Tujia

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00286

text: Pakistan

text: Homo sapiens

text: Kalash

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01251

text: China

text: Homo sapiens

text: Xibo

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01209

text: China

text: Homo sapiens

text: Oroqen

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01334

text: China

text: Homo sapiens

text: She

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00192

text: Pakistan

text: Homo sapiens

text: Sindhi

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00987

text: Namibia

text: Homo sapiens

text: San

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00544

text: New Guinea

text: Homo sapiens

text: Papuan

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01354

text: China

text: Homo sapiens

text: Tu

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00471

text: Congo

text: Homo sapiens

text: Mbuti

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00909

text: Senegal

text: Homo sapiens

text: Mandenka

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01215

text: China

text: Homo sapiens

text: Daur

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00557

text: Israel

text: Homo sapiens

text: Druze

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00151

text: Pakistan

text: Homo sapiens

text: Makrani

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01363

text: France

text: Homo sapiens

text: French Basque

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01314

text: China

text: Homo sapiens

text: Dai

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00830

text: Brazil

text: Homo sapiens

text: Surui

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00920

text: Nigeria

text: Homo sapiens

text: Yoruba

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01223

text: China

text: Homo sapiens

text: Mongola

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00754

text: Japan

text: Homo sapiens

text: Japanese

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00096

text: Pakistan

text: Homo sapiens

text: Balochi

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00794

text: Orkney Islands

text: Homo sapiens

text: Orcadian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00702

text: Colombia

text: Homo sapiens

text: Colombian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00995

text: Brazil

text: Homo sapiens

text: Karitiana

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01381

text: Russia

text: Homo sapiens

text: Adygei

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01038

text: Mexico

text: Homo sapiens

text: Pima

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00955

text: Siberia

text: Homo sapiens

text: Yakut

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00656

text: Bougainville

text: Homo sapiens

text: NAN Melanesian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00854

text: Mexico

text: Homo sapiens

text: Maya

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01234

text: China

text: Homo sapiens

text: Hezhen

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01414

text: Kenya

text: Homo sapiens

text: Bantu

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01323

text: China

text: Homo sapiens

text: Lahu

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00015

text: Pakistan

text: Homo sapiens

text: Brahui

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01254

text: Algeria

text: Homo sapiens

text: Mozabite

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00232

text: Pakistan

text: Homo sapiens

text: Pathan

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP00712

text: Cambodia

text: Homo sapiens

text: Cambodian

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: Human CEPH-HGDP individual HGDP01188

text: China

text: Homo sapiens

text: Yizu

text: Homo sapiens


text: BI-UK

text: public

text: ES

characteristic: cell type

text: house mouse

text: Mock KD ES 3

text: Mock KD

characteristic: genotype

text: Mus musculus

characteristic: replicate

text: Mus musculus


name: E-MTAB-714:D immitis adult male_version2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ED

text: public

text: ArrayExpress

characteristic: broker name

text: dog heartworm nematode

text: Protocols: RNA was extracted from the worms using the protocol described in Qiagen miRNeasy Mini Handbook October 2007. Several steps were carried out to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing. The first step involves purifying the poly-A containing mRNA molecules using poly-T oligo-attached magnetic beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then, the cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and a high concentration of random hexamer primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. Finally, the short cDNA fragments are prepared for sequencing on the Illumina Genome Analyzer IIx.

text: adult

characteristic: developmental stage

text: Dirofilaria immitis

text: Dirofilaria immitis

text: Dirofilaria immitis

text: male

characteristic: sex

text: D immitis adult male

characteristic: title


name: E-MTAB-714:D immitis adult female_version2

text: ED

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: dog heartworm nematode

characteristic: description

text: adult

text: Dirofilaria immitis

text: Dirofilaria immitis

text: Dirofilaria immitis

text: female

text: D immitis adult female


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: SRA accession

text: MGH_cross__AB_G0__NspI_digest__B_-sc-2010-05-07-1163

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: AB GO (grandmother) of the MGH meiotic cross. NspI cut, ligated with modified recuttable adaptor (ATTATGAGCACGACAGACGCCTGATCTRCATG and YAGATCAGGCGTCTGTCGTGCTCATAA), PCR amplification reaction B (as if Affymtrix NspI SNP5 protocol), recut with NspI, blunt ended, 200-400bp size selected and turned into standard Illumina library.

characteristic: sample description

text: Danio rerio

text: AB (MGH)

characteristic: strain


text: SC

text: public

text: MGH_cross__India_G0__NspI_digest__A_-sc-2010-09-13-1159

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: Danio rerio


characteristic: External Id

text: SC

text: public

text: MGH_cross__India_G0__NspI_digest__B_-sc-2010-09-13-1160

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__India_G0__NspI_digest__C_-sc-2010-09-13-1161

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__AB_G0__NspI_digest__A_-sc-2010-09-13-1162

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__AB_G0__NspI_digest__C_-sc-2010-09-13-1164

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__F1_female__NspI_digest__B_-sc-2010-09-13-1166

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__F1_female__NspI_digest__C_-sc-2010-09-13-1167

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__F1_male__NspI_digest__B_-sc-2010-09-13-1169

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__F1_male__NspI_digest__C_-sc-2010-09-13-1170

text: zebrafish

text: Danio rerio

text: Danio rerio


text: SC

text: public

text: MGH_cross__F1_male__NspI_digest__A_-sc-2010-09-13-1168

text: zebrafish

text: Danio rerio

text: Danio rerio


characteristic: ENA-LAST-UPDATE

text: GATC

text: public

characteristic: StrainOrLine

text: 7 to 9 weeks

characteristic: age

text: ArrayExpress

characteristic: broker name

text: dendritic

characteristic: cell type

text: house mouse

text: Protocols: Candida albicans was grown in 1% yeast extract, 2% peptone, and 2% glucose and collected in the logarithmic growth. Dendritic cells were derived from C57BL/6 femur bone marrow in DMEM supplemented with 10% FCS, and 15 % GM-CSF. Dendritic cell and Candida albicans coculture at a target ratio of 1:1 Samples were incubated at 37C 5% CO2, 95% humidity between 0 and 120 min. We isolated total RNA from samples using the SV total RNA isolation system (Promega). Cells were scraped in the provided lysis buffer followed by homogenization with 0.5 mm acid washed glass beads. Extraction was performed following manufactures protocols.

text: Candida albicans

text: Mus musculus

text: Mus Musculus

text: Mus musculus

text: male


Anderseniella sp. Alg231_50

characteristic: ENA-CHECKLIST

text: Elham Karimi

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Anderseniella sp. Alg231_50

tag: Namespace:Elham Karimi

characteristic: collection date

text: Ocean

characteristic: environment (biome)

text: Coastal water body

characteristic: environment (feature)

text: sea water

characteristic: environment (material)

text: Portugal

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Marine Sponge

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Anderseniella sp. Alg231-50

text: Sponge-associated Alphaproteobacteria

characteristic: project name

text: Anderseniella sp. Alg231-50

text: Illumina

characteristic: sequencing method

text: Sponge-associated Alphaproteobacteria

characteristic: title


Erythrobacter sp. Alg231_14

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Elham Karimi

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Erythrobacter sp. Alg231_14

characteristic: Submitter Id

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Erythrobacter sp. Alg231-14

characteristic: organism

text: Sponge-associated Alphaproteobacteria

text: Erythrobacter sp. Alg231-14

text: Illumina

text: Sponge-associated Alphaproteobacteria


Labrenzia sp. Alg231_36

characteristic: ENA-CHECKLIST

text: Elham Karimi

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Labrenzia sp. Alg231_36

characteristic: collection date

text: Ocean

characteristic: environment (biome)

text: Coastal water body

characteristic: environment (feature)

text: sea water

characteristic: environment (material)

text: Portugal

characteristic: geographic location (country and/or sea)

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Labrenzia sp. Alg231-36

text: Sponge-associated Alphaproteobacteria

text: Labrenzia sp. Alg231-36

text: Illumina

text: Sponge-associated Alphaproteobacteria


Sphingorhabdus sp. Alg231_15

text: Elham Karimi

text: public

text: Sphingorhabdus sp. Alg231_15

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Marine Sponge

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Sphingorhabdus sp. Alg231-15

text: Sponge-associated Alphaproteobacteria

characteristic: project name

text: Sphingorhabdus sp. Alg231-15

text: Illumina

characteristic: sequencing method

text: Sponge-associated Alphaproteobacteria

characteristic: title


Pseudovibrio sp. Alg231_02

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Elham Karimi

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Pseudovibrio sp. Alg231_02

characteristic: Submitter Id

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Pseudovibrio sp. Alg231-02

characteristic: organism

text: Sponge-associated Alphaproteobacteria

text: Pseudovibrio sp. Alg231-02

text: Illumina

text: Sponge-associated Alphaproteobacteria


Rhodobacteraceae bacterium Alg231_30

characteristic: ENA-CHECKLIST

text: Elham Karimi

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Rhodobacteraceae bacterium Alg231_30

characteristic: collection date

text: Ocean

characteristic: environment (biome)

text: Coastal water body

characteristic: environment (feature)

text: sea water

characteristic: environment (material)

text: Portugal

characteristic: geographic location (country and/or sea)

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Rhodobacteraceae bacterium Alg231-30

text: Sponge-associated Alphaproteobacteria

text: Rhodobacteraceae bacterium Alg231-30

text: Illumina

text: Sponge-associated Alphaproteobacteria


Rhodobacteraceae bacterium Alg231_04

text: Elham Karimi

text: public

text: Rhodobacteraceae bacterium Alg231_04

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Marine Sponge

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Rhodobacteraceae bacterium Alg231-04

text: Sponge-associated Alphaproteobacteria

characteristic: project name

text: Rhodobacteraceae bacterium Alg231-04

text: Illumina

characteristic: sequencing method

text: Sponge-associated Alphaproteobacteria

characteristic: title


Ruegeria sp. Alg231_54

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Elham Karimi

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Ruegeria sp. Alg231_54

characteristic: Submitter Id

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Ruegeria sp. Alg231-54

characteristic: organism

text: Sponge-associated Alphaproteobacteria

text: Ruegeria sp. Alg231-54

text: Illumina

text: Sponge-associated Alphaproteobacteria


Loktanella sp. Alg231_35

characteristic: ENA-CHECKLIST

text: Elham Karimi

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Loktanella sp. Alg231_35

characteristic: collection date

text: Ocean

characteristic: environment (biome)

text: Coastal water body

characteristic: environment (feature)

text: sea water

characteristic: environment (material)

text: Portugal

characteristic: geographic location (country and/or sea)

text: Marine Sponge

text: host-associated

text: mimarks-survey

text: Loktanella sp. Alg231-35

text: Sponge-associated Alphaproteobacteria

text: Loktanella sp. Alg231-35

text: Illumina

text: Sponge-associated Alphaproteobacteria


Tateyamaria sp. Alg231_49

text: Elham Karimi

text: public

text: Tateyamaria sp. Alg231_49

text: Ocean

text: Coastal water body

text: sea water

text: Portugal

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Marine Sponge

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Tateyamaria sp. Alg231-49

text: Sponge-associated Alphaproteobacteria

characteristic: project name

text: Tateyamaria sp. Alg231-49

text: Illumina

characteristic: sequencing method

text: Sponge-associated Alphaproteobacteria

characteristic: title


characteristic: External Id

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: ISE

characteristic: strain


text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus


text: SC

text: public

text: BG_mHPC7_Runx1_170209-sc-2010-03-04-2648

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: m_Brain_CFP1Skallib_M-sc-2010-03-05-10620

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: IT


text: SC

text: public

text: pd_MouseWtypeT_rabbit_IgG-sc-2010-03-04-4793

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: pd_HPC7_Bcl11a_pcanti-sc-2010-03-04-4790

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: BG_mHPC7_Meis1_210109-sc-2010-03-04-2647

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: pd_MouseWtypeT_Bcl11b_pcanti-sc-2010-03-04-4792

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus


text: SC

text: public

text: human

text: Homo sapiens

text: Cultured human erythroleukemic cell line K562

characteristic: sample description

text: Homo sapiens


text: SC

text: public

text: bats

text: Chiroptera

text: Chiroptera


text: SC

text: public

text: human

text: Homo sapiens

text: MeDIP troubleshooting

text: Homo sapiens


text: SC

text: public

text: zebrafish

text: Danio rerio

text: Danio rerio

text: TL


text: Xenopus tropicalis

characteristic: ArrayExpress-Species

text: SC

text: public

text: XTEP5092984-sc-2011-04-13T17:40:55Z-1115371

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083832-sc-2011-04-13T17:38:34Z-1105763

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083835-sc-2011-04-13T17:38:36Z-1105766

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092987-sc-2011-04-13T17:40:57Z-1115374

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092990-sc-2011-04-13T17:41:00Z-1115377

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083838-sc-2011-04-13T17:38:38Z-1105769

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083841-sc-2011-04-13T17:38:40Z-1105772

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092945-sc-2011-04-13T17:40:30Z-1115332

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092948-sc-2011-04-13T17:40:32Z-1115335

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092993-sc-2011-04-13T17:41:01Z-1115380

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092996-sc-2011-04-13T17:41:04Z-1115383

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083844-sc-2011-04-13T17:38:42Z-1105775

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092999-sc-2011-04-13T17:41:05Z-1115386

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083847-sc-2011-04-13T17:38:44Z-1105778

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083850-sc-2011-04-13T17:38:46Z-1105781

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083856-sc-2011-04-13T17:38:50Z-1105787

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093002-sc-2011-04-13T17:41:07Z-1115389

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093005-sc-2011-04-13T17:41:09Z-1115392

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083859-sc-2011-04-13T17:38:52Z-1105790

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083862-sc-2011-04-13T17:38:55Z-1105793

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083865-sc-2011-04-13T17:38:57Z-1105796

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093017-sc-2011-04-13T17:41:17Z-1115404

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093020-sc-2011-04-13T17:41:19Z-1115407

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083868-sc-2011-04-13T17:38:58Z-1105799

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083871-sc-2011-04-13T17:39:02Z-1105802

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093008-sc-2011-04-13T17:41:11Z-1115395

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093023-sc-2011-04-13T17:41:21Z-1115410

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083874-sc-2011-04-13T17:39:03Z-1105805

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083877-sc-2011-04-13T17:39:06Z-1105808

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093011-sc-2011-04-13T17:41:14Z-1115398

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083880-sc-2011-04-13T17:39:07Z-1105811

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083883-sc-2011-04-13T17:39:10Z-1105814

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092951-sc-2011-04-13T17:40:33Z-1115338

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092954-sc-2011-04-13T17:40:35Z-1115341

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083886-sc-2011-04-13T17:39:12Z-1105817

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083889-sc-2011-04-13T17:39:15Z-1105820

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092957-sc-2011-04-13T17:40:37Z-1115344

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092960-sc-2011-04-13T17:40:39Z-1115347

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083892-sc-2011-04-13T17:39:17Z-1105823

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092963-sc-2011-04-13T17:40:42Z-1115350

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092966-sc-2011-04-13T17:40:44Z-1115353

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083898-sc-2011-04-13T17:39:21Z-1105829

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092969-sc-2011-04-13T17:40:46Z-1115356

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083901-sc-2011-04-13T17:39:23Z-1105832

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083904-sc-2011-04-13T17:39:25Z-1105835

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092972-sc-2011-04-13T17:40:48Z-1115359

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092975-sc-2011-04-13T17:40:50Z-1115362

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092978-sc-2011-04-13T17:40:52Z-1115365

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092981-sc-2011-04-13T17:40:54Z-1115368

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083829-sc-2011-04-13T17:38:31Z-1105760

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083857-sc-2011-04-13T17:38:51Z-1105788

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083860-sc-2011-04-13T17:38:53Z-1105791

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093012-sc-2011-04-13T17:41:14Z-1115399

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093015-sc-2011-04-13T17:41:16Z-1115402

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083863-sc-2011-04-13T17:38:55Z-1105794

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083866-sc-2011-04-13T17:38:57Z-1105797

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093018-sc-2011-04-13T17:41:18Z-1115405

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093021-sc-2011-04-13T17:41:20Z-1115408

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083869-sc-2011-04-13T17:39:00Z-1105800

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083872-sc-2011-04-13T17:39:02Z-1105803

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093024-sc-2011-04-13T17:41:22Z-1115411

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083875-sc-2011-04-13T17:39:04Z-1105806

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083878-sc-2011-04-13T17:39:06Z-1105809

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083881-sc-2011-04-13T17:39:08Z-1105812

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083884-sc-2011-04-13T17:39:11Z-1105815

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092943-sc-2011-04-13T17:40:28Z-1115330

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083887-sc-2011-04-13T17:39:13Z-1105818

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083890-sc-2011-04-13T17:39:15Z-1105821

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092946-sc-2011-04-13T17:40:31Z-1115333

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083893-sc-2011-04-13T17:39:18Z-1105824

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083896-sc-2011-04-13T17:39:19Z-1105827

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092952-sc-2011-04-13T17:40:34Z-1115339

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092955-sc-2011-04-13T17:40:36Z-1115342

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083899-sc-2011-04-13T17:39:22Z-1105830

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083902-sc-2011-04-13T17:39:24Z-1105833

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092958-sc-2011-04-13T17:40:38Z-1115345

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092961-sc-2011-04-13T17:40:40Z-1115348

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092964-sc-2011-04-13T17:40:42Z-1115351

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092967-sc-2011-04-13T17:40:45Z-1115354

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092970-sc-2011-04-13T17:40:47Z-1115357

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092973-sc-2011-04-13T17:40:48Z-1115360

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092976-sc-2011-04-13T17:40:50Z-1115363

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092979-sc-2011-04-13T17:40:52Z-1115366

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083830-sc-2011-04-13T17:38:32Z-1105761

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092982-sc-2011-04-13T17:40:54Z-1115369

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092985-sc-2011-04-13T17:40:56Z-1115372

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083833-sc-2011-04-13T17:38:34Z-1105764

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083836-sc-2011-04-13T17:38:37Z-1105767

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092988-sc-2011-04-13T17:40:58Z-1115375

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092991-sc-2011-04-13T17:41:00Z-1115378

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083839-sc-2011-04-13T17:38:39Z-1105770

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083842-sc-2011-04-13T17:38:41Z-1105773

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092994-sc-2011-04-13T17:41:02Z-1115381

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5092997-sc-2011-04-13T17:41:04Z-1115384

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083845-sc-2011-04-13T17:38:43Z-1105776

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083848-sc-2011-04-13T17:38:45Z-1105779

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093000-sc-2011-04-13T17:41:06Z-1115387

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093003-sc-2011-04-13T17:41:08Z-1115390

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Mus musculus

text: SC

text: public

text: CDTCIC5083851-sc-2011-04-13T17:38:47Z-1105782

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: CDTCIC5083854-sc-2011-04-13T17:38:49Z-1105785

text: house mouse

text: Mus musculus

text: Mus musculus


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093006-sc-2011-04-13T17:41:10Z-1115393

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: XTEP5093009-sc-2011-04-13T17:41:12Z-1115396

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232334-sc-2011-10-04T11:18:03Z-1284613

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232340-sc-2011-10-04T11:18:08Z-1284619

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232349-sc-2011-10-04T11:18:17Z-1284628

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232355-sc-2011-10-04T11:18:22Z-1284634

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232364-sc-2011-10-04T11:18:30Z-1284643

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232352-sc-2011-10-04T11:18:20Z-1284631

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232361-sc-2011-10-04T11:18:27Z-1284640

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232372-sc-2011-10-04T11:18:36Z-1284651

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232350-sc-2011-10-04T11:18:18Z-1284629

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232335-sc-2011-10-04T11:18:04Z-1284614

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232341-sc-2011-10-04T11:18:09Z-1284620

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232336-sc-2011-10-04T11:18:05Z-1284615

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232339-sc-2011-10-04T11:18:07Z-1284618

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232342-sc-2011-10-04T11:18:10Z-1284621

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232348-sc-2011-10-04T11:18:14Z-1284627

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232333-sc-2011-10-04T11:18:02Z-1284612

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232362-sc-2011-10-04T11:18:28Z-1284641

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232353-sc-2011-10-04T11:18:20Z-1284632

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232356-sc-2011-10-04T11:18:23Z-1284635

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232368-sc-2011-10-04T11:18:34Z-1284647

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232369-sc-2011-10-04T11:18:34Z-1284648

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: SC

text: public

text: western wild mouse

text: Mus spretus

text: Mus spretus

text: SPRET/EiJ


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: NOD/ShiLtJ


text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: ISE


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: human

text: Homo sapiens

text: Smooth Muscle Cell histone lysine 9 methylation by ChIP

text: Homo sapiens


text: Sox2CTAP 2-steps ChIP

text: Mus musculus E14 ESC

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Sox2CTAP_2_steps_ChIP-sc-2013-02-20T12:46:55Z-42747

text: house mouse

text: Mus musculus

text: Capturing the tagged protein and associated factors/ genomic DNA using anti-FLAG M2 monoclonal antibody coated on beads. Purified complexes are released from the beads using FLAG peptide. Further purification with calmodulin resin, from which the complexes are eluted by EGTA.

text: Mus musculus

text: Mus musculus E14 ESC


text: LacZCTAP 2-steps ChIP

text: Mus musculus E14 ESC

text: SC

text: public

text: LacZCTAP_2_steps_ChIP-sc-2013-02-20T12:47:14Z-42748

text: house mouse

text: Mus musculus

text: Negative control. Capturing the tagged protein and associated factors/ genomic DNA using anti-FLAG M2 monoclonal antibody coated on beads. Purified complexes are released from the beads using FLAG peptide. Further purification with calmodulin resin, from which the complexes are eluted by EGTA.

text: Mus musculus

text: Mus musculus E14 ESC


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232383-sc-2011-10-04T11:18:46Z-1284662

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: SC_XTEP5232395-sc-2011-10-04T11:18:56Z-1284674

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: SC

text: public

text: human

text: Homo sapiens

text: Smooth Muscle Cell histone lysine 9 tri-methylation by ChIP

text: Homo sapiens


text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus


text: SC

text: public

text: human

text: Homo sapiens

text: Cultured human erythroleukemic cell line K562

text: Homo sapiens


text: SC

text: public

text: human

text: Homo sapiens

text: Smooth Muscle Cell histone lysine 4 methylation by ChIP

text: Homo sapiens


text: Danio rerio

text: TL

text: SC

text: public

text: SL3_01_A3-sc-2011-04-11T09:28:59Z-1119614

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL


text: Danio rerio

text: TL

text: SC

text: public

text: SL3_01_B4-sc-2011-04-11T09:29:04Z-1119632

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL


text: Danio rerio

text: TL

text: SC

text: public

text: SL3_01_A6-sc-2011-04-11T09:29:13Z-1119663

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL


text: Danio rerio

text: TL

text: SC

text: public

text: SL3_01_B1-sc-2011-04-11T09:28:50Z-1119592

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL


text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

text: SC

text: public

text: SC_GMJOL5309796-sc-2012-08-22T15:40:47Z-1376395

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMJOL5309799-sc-2012-08-22T15:40:49Z-1376398

text: human

text: Homo sapiens

text: Homo sapiens


text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae


text: Mus Musculus

text: SC

text: public

text: 2042STDY5305476-sc-2012-03-05T08:22:09Z-1370446

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: 2042STDY5305479-sc-2012-03-05T08:22:17Z-1370449

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: 2042STDY5305482-sc-2012-03-05T08:22:21Z-1370452

text: house mouse

text: Mus musculus

text: Mus musculus


text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


status: KILLED

text: adult

characteristic: DevelopmentalStage

text: CRUK-CRI

text: killed

text: Canis lupus familiaris

characteristic: Organism

text: liver

characteristic: OrganismPart

text: male

characteristic: Sex

text: ArrayExpress

characteristic: broker name

text: dog

text: Protocols: Samples were prepared by direct perfusion of the tissue with buffered salt solution, followed by % formaldehyde. After 10 minutes, the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization, the cells were rinsed with PBS. The animal tissues were crosslinked with formaldehyde treatment and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA - version 2.2) with the following modifications. The ChIP-enriched DNA was not further fragmented. After end-repair and addition of an 'A' base to the 3' ends, the adapters were ligated to the ends of the DNA Fragments using 2 ul of fortyfold diluted 'Adapter oligo mix' in a total reaction volume of 25 ul. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5 ul of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2% agarose gel and a gel slice containing the 200-300 bp fragments was excised. The flowcells were prepared and processed according to the manufacturer's protocols, with single-end sequencing for 36 to 45 cycles.

characteristic: description

text: Canis lupus familiaris

text: Canis lupus familiaris

characteristic: title