characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

text: central memory CD4-positive, alpha-beta T cell

characteristic: cell type

text: human

text: Protocols: Isolation of human CD4+ T cells: Isolation of leukocytes from Human skin: Plastic surgery skin included reticular dermis to the depth of the fat layer. The upper 200 microns of skin were harvested using a split skin graft knife. Whole skin was digested in RF10 with 1.6mg/ml type IV collagenase for 12-16 hours at 37°C and 5%CO2. Digest was passed repeatedly through a 10ml pipette until no visible material remained. To yield a single cell suspension, digest was passed through a 100-micron filter into a polypropylene sorting tube. Wells were washed twice using cold sort buffer without calcium or magnesium to collect residual and adherent cells.Isolation of leukocytes from Human colon:Normal regions of bowel adjacent to resected colorectal tumours were prepared as previously described, with minor modifications (Bettelli et al., 2006; Geremia et al., 2011). In brief, mucosa was dissected and washed in 1 mM dithiothreitol (DTT) solution for 15 min at room temperature to remove mucus. Specimens were then washed three times in 0.75 mM EDTA to deplete epithelial crypts and were digested for 2h in 0.1 mg/ml collagenase A solution (Roche, UK). For enrichment of mononuclear cells, digests were centrifuged for 30 min at 500g in a four-layer Percoll gradient and collected at the 40%/60% interface.Peripheral blood mononuclear cell isolation: 10ml blood from skin donors were collected into EDTA. Density centrifugation with Lymphoprep was performed according to manufacturer instructions. Recovered cells were cryopreserved by pelleting and resuspending in 1ml heat-inactivated fetal calf serum containing 10% DMSO, and storing at -80ºC. Cryovials were later thawed in water bath, then rapidly being transferred to warmed medium (RPMI 1640 with 100IU/ml penicillin, 10ug/ml streptomycin, 2mM L-glutamine, 10% heat-inactivated fetal calf serum) and filtered through a 100-micron filter. Mouse and human cell suspensions were stained with the antibodies in Supplementary Table 1 and DAPI. Single-cells were sorted in 2μl of Lysis Buffer (1:20 solution of RNase Inhibitor (Clontech, cat. no. 2313A) in 0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284)) in 96 well plates, spun down and immediately frozen at -80 degrees. Smart-seq2 protocol was largely followed to obtain mRNA libraries from single-cells. Oligo-dT primer, dNTPs (ThermoFisher, cat. no. 10319879) and ERCC RNA Spike-In Mix (1:50,000,000 final dilution, Ambion, cat. no. 4456740) were then added. Reverse Transcription and PCR were performed as previously published21, using 50U of SMARTScribe™ Reverse Transcriptase (Clontech, cat. no. 639538). The cDNA libraries for sequencing were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, cat. no. FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1).

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: total reads

characteristic: unmapped reads (percent)


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: false

text: Homo sapiens

text: female

characteristic: title


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: false

text: Homo sapiens

text: male

characteristic: unmapped reads (percent)


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: true

text: Homo sapiens

text: female

characteristic: total reads


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: skin

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

text: true

text: Homo sapiens

text: female

characteristic: title


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

text: effector memory CD4-positive, alpha-beta T cell

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: false

text: Homo sapiens

text: female

characteristic: unmapped reads (percent)


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Homo sapiens

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: true

text: Homo sapiens

text: male

characteristic: total reads


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: false

text: Homo sapiens

text: female

characteristic: title


text: Wellcome Trust Sanger Institute

text: public

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

text: normal

characteristic: disease

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: skin

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

text: false

text: Homo sapiens

text: female

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

text: Protocols: Mouse melanoma induction: The melanoma induction experiments were performed in accordance with UK Home Office regulations under Project License PPL 80/2574. Protocol used was adapted from a previous publication (Riedel et al., 2016). For syngeneic tumours, 2.5 × 105 B16.F10 melanoma cells were inoculated subcutaneously into the shoulder region 6- to 14-week-old female Foxp3-IRES-GFP mice (Haribhai et al., 2007). Animals were excluded only if tumours failed to form or if health concerns were reported. Control Foxp3-IRES-GFP mice were injected with 50 μl PBS. Animals were culled after 11 days. Tumour tissue, tumour-draining (brachial) lymph nodes and spleen were isolated for subsequent analysis. PBS-injected and steady-state skin, draining lymph nodes (bLN) and spleen were collected from control mice. Tumour and PBS-injected skin were mechanically disrupted and digested in a 1ml mixture of 1 mg/ml collagenase A (Roche) and 0.4 mg/ml DNase I (Roche) in PBS (solution A) at 37°C for 1h with 600rpm rotation. 1ml of PBS containing 1mg/ml Collagenase D (Roche) and 0.4 mg/ml DNase I (solution B) was then added to each sample, which returned to 37 °C for 1h with 600 rpm rotation. Lymph nodes were digested for 30min in 500μl of solution A, and for further 30min after the addition of 500μl of solution B. EDTA at the final concentration of 10mM was added to all samples. Spleens were processed as described previously (Uhlig et al., 2006). Suspensions were passed through a 70 µm mesh before immunostaining with combinations of fluorescently conjugated antibodies. Samples from different animals were kept separated throughout processing and sorting. Cells from PBS-injected and steady-state skin were compared and concluded to be equivalent in expression. Mouse and human cell suspensions were stained with the antibodies in Supplementary Table 1 and DAPI. Single-cells were sorted in 2μl of Lysis Buffer (1:20 solution of RNase Inhibitor (Clontech, cat. no. 2313A) in 0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284)) in 96 well plates, spun down and immediately frozen at -80 degrees. Smart-seq2 protocol was largely followed to obtain mRNA libraries from single-cells. Oligo-dT primer, dNTPs (ThermoFisher, cat. no. 10319879) and ERCC RNA Spike-In Mix (1:50,000,000 final dilution, Ambion, cat. no. 4456740) were then added. Reverse Transcription and PCR were performed as previously published21, using 50U of SMARTScribe™ Reverse Transcriptase (Clontech, cat. no. 639538). The cDNA libraries for sequencing were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, cat. no. FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1).

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

text: TCRb-positive, CD44-high, Foxp3-negative

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brachial lymph node

text: false

text: Mus musculus

text: female

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brachial lymph node

text: true

text: Mus musculus

text: male

characteristic: side scatter FACS measurement

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brachial lymph node

text: true

text: Mus musculus

text: female

characteristic: side scatter FACS measurement

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: melanoma

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Isolation of mouse CD4+ T cells: Isolation of murine leukocytes for steady-state skin dataset: To isolate leukocytes from ear tissue, ears were removed at the base, split into halves and cut into very small pieces. Tissue was digested in 3.5ml RPMI media with 0.1% BSA, 15mM Hepes, 1mg/ml collagenase D (Roche) and 450μg/ml Liberase TL (Roche) for 60 minutes at 37°C in a shaking incubator at 200rpm. Digested tissue was passed through an 18G needle to further disrupt the tissue and release cells. Cells were passed through a 70μm cell strainer, and the digestion was terminated by addition of ice-cold RPMI containing 0.1%BSA and 5mM EDTA. A three-layer (30/40/70%) Percoll density-gradient was used to enrich for the lymphocytes. Cells obtained from the digestion were layered in the 30% layer on top of the 40% and 70% layers, and centrifuged for 20 minutes at 1800rpm without brake. Cells at the 40/70% interface were collected for the subsequent analysis. Cell suspensions from spleen and bLN were prepared as described previously (Uhlig et al., 2006). Isolation of murine leukocytes for steady-state colon dataset: Colons were washed twice in RPMI media with 0.1%BSA and 5mM EDTA in a shaking incubator at 200rpm at 37°C to remove epithelial cells. The tissue was then digested for an hour in the presence of RPMI/10%FCS/15mM Hepes with 100U/ml collagenase VIII. Digestion was terminated by addition of ice-cold RPMI/10% FCS/5mM EDTA. A three-layer (30/40/70%) Percoll density-gradient was used to enrich for the lymphocytes. Cells obtained from the digestion were layered in the 30% layer on top of the 40% and 70% layers, and centrifuged for 20 minutes at 1800rpm without brake. Cells at the 40/70% interface were collected for the subsequent analysis. Cell suspensions from spleen and mLN were prepared as described previously (Uhlig et al., 2006). Mouse and human cell suspensions were stained with the antibodies in Supplementary Table 1 and DAPI. Single-cells were sorted in 2μl of Lysis Buffer (1:20 solution of RNase Inhibitor (Clontech, cat. no. 2313A) in 0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284)) in 96 well plates, spun down and immediately frozen at -80 degrees. Smart-seq2 protocol was largely followed to obtain mRNA libraries from single-cells. Oligo-dT primer, dNTPs (ThermoFisher, cat. no. 10319879) and ERCC RNA Spike-In Mix (1:50,000,000 final dilution, Ambion, cat. no. 4456740) were then added. Reverse Transcription and PCR were performed as previously published21, using 50U of SMARTScribe™ Reverse Transcriptase (Clontech, cat. no. 639538). The cDNA libraries for sequencing were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, cat. no. FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1).

characteristic: description

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: true

text: Mus musculus

text: not available

characteristic: side scatter FACS measurement

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

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text: not available

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

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characteristic: INSDC center name

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: false

text: Mus musculus

text: not available

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

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characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

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text: Mus musculus

text: lymph node

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text: false

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characteristic: sex

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characteristic: External Id

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characteristic: INSDC center name

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

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text: Mus musculus

text: lymph node

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text: Mus musculus

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characteristic: total reads

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characteristic: genotype

text: not available

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text: Mus musculus

text: Mus musculus

text: mesenteric lymph node

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text: true

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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

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characteristic: INSDC center name

characteristic: INSDC first public

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

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text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mesenteric lymph node

text: false

text: Mus musculus

text: not available

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

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characteristic: ENA-CHECKLIST

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characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: mesenteric lymph node

characteristic: organism part

characteristic: phenotype

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characteristic: position in library

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text: false

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text: not available

characteristic: sex

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characteristic: strain

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

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text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

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text: normal

characteristic: disease

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characteristic: organism

text: Mus musculus

text: skin

text: false

text: Mus musculus

text: female

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

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characteristic: ENA-CHECKLIST

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characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

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characteristic: library

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text: Mus musculus

text: skin

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characteristic: phenotype

characteristic: plate

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text: true

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text: female

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

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characteristic: cell type

text: house mouse

characteristic: common name

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text: normal

characteristic: disease

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characteristic: organism

text: Mus musculus

text: skin

text: false

text: Mus musculus

text: male

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

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characteristic: ERCC measurement (percent)

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text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

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text: Mus musculus

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characteristic: phenotype

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characteristic: position in library

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text: true

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characteristic: ERCC measurement (percent)

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characteristic: INSDC center name

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characteristic: age

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characteristic: broker name

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characteristic: cell type

text: house mouse

characteristic: common name

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text: normal

characteristic: disease

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characteristic: organism

text: Mus musculus

text: skin

text: true

text: Mus musculus

text: male

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

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characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

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text: Mus musculus

text: skin

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characteristic: position in library

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text: true

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text: not available

characteristic: sex

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characteristic: Cd4 FACS measurement

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

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characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: false

text: Mus musculus

text: not available

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

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text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

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text: Mus musculus

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text: false

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characteristic: ERCC measurement (percent)

characteristic: External Id

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characteristic: INSDC center name

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text: public

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characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: true

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text: female

characteristic: side scatter FACS measurement

characteristic: total reads

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characteristic: ERCC measurement (percent)

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text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

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characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: normal

characteristic: genotype

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text: spleen

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characteristic: phenotype

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text: true

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

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characteristic: INSDC center name

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text: 6 to 14

characteristic: age

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characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

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text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: false

text: Mus musculus

text: male

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

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text: house mouse

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characteristic: forward scatter FACS measurement

characteristic: genotype

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text: false

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characteristic: ERCC measurement (percent)

characteristic: External Id

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characteristic: INSDC center name

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characteristic: Klrg1 FACS measurement

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characteristic: Submitter Id

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

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text: house mouse

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text: spleen

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text: Mus musculus

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characteristic: total reads

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characteristic: ENA-CHECKLIST

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characteristic: ERCC measurement (percent)

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text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

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characteristic: genotype

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text: Mus musculus

text: spleen

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text: false

characteristic: post analysis well quality

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

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text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

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text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

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characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

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characteristic: cell type

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text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: false

text: Mus musculus

text: not available

characteristic: side scatter FACS measurement

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characteristic: INSDC center name

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characteristic: INSDC last update

text: public

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characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

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text: not available

characteristic: individual

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text: spleen

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characteristic: position in library

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text: true

characteristic: post analysis well quality

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text: not available

characteristic: sex

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characteristic: strain

characteristic: title


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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: INSDC status

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: false

text: Mus musculus

text: not available

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: melanoma

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

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text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

characteristic: phenotype

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characteristic: position in library

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text: true

characteristic: post analysis well quality

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characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

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text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

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characteristic: INSDC last update

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text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: false

text: Mus musculus

text: female

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: CD4-positive, alpha-beta memory T cell

text: house mouse

text: melanoma

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD4-positive, alpha-beta memory T cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: false

text: Mus musculus

text: male

characteristic: total reads

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: not available

text: ArrayExpress

text: CD4-positive, CD25-positive, alpha-beta regulatory T cell

text: human

text: normal

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: title


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: false

text: Homo sapiens

text: female

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: not available

text: ArrayExpress

text: human

text: normal

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: unmapped reads (percent)


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: false

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: not available

text: ArrayExpress

text: human

text: normal

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: total reads


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: colon

text: false

text: Homo sapiens

text: female

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: not available

text: ArrayExpress

text: human

text: normal

characteristic: individual

characteristic: library

characteristic: library date

text: Homo sapiens

text: Homo sapiens

text: skin

characteristic: organism part

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Homo sapiens

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: title


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

text: false

text: Homo sapiens

text: female

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brachial lymph node

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: female

characteristic: side scatter FACS measurement

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brachial lymph node

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: female

characteristic: side scatter FACS measurement

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: melanoma

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: brachial lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: not available

characteristic: side scatter FACS measurement

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: not available

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: not available

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: not available

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: not available

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: mesenteric lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: not available

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mesenteric lymph node

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: not available

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: mesenteric lymph node

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: not available

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: female

characteristic: title

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

text: Wellcome Trust Sanger Institute

text: public

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

characteristic: forward scatter FACS measurement

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: female

characteristic: side scatter FACS measurement

characteristic: title

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: male

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: not available

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: not available

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

text: not available

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: not available

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: female

characteristic: title

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: house mouse

text: melanoma

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: male

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: melanoma

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: skin

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: melanoma

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: male

characteristic: title

characteristic: total reads


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

text: public

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: Sell FACS measurement

characteristic: Trbc1 FACS measurement

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: forward scatter FACS measurement

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: true

characteristic: post analysis well quality

text: Mus musculus

text: male

characteristic: sex

characteristic: side scatter FACS measurement

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: Cd4 FACS measurement

characteristic: Cd44 FACS measurement

characteristic: DAPI FACS measurement

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

characteristic: Foxp3-GFP FACS measurement

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Il1rl1 FACS measurement

characteristic: Itgae FACS measurement

characteristic: Klrg1 FACS measurement

characteristic: SRA accession

characteristic: Sell FACS measurement

characteristic: Submitter Id

characteristic: Trbc1 FACS measurement

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: TCRb-positive, Foxp3-positive

text: true

text: Mus musculus

text: female

characteristic: title

characteristic: total reads


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 6 to 14

text: ArrayExpress

text: house mouse

text: normal

characteristic: genotype

characteristic: individual

characteristic: library

characteristic: library date

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: TCRb-positive, Foxp3-positive

characteristic: phenotype

characteristic: plate

characteristic: position in library

characteristic: position in smart-seq2

text: false

characteristic: post analysis well quality

text: Mus musculus

text: female

characteristic: sex

characteristic: smart-seq2 date

characteristic: strain

characteristic: unmapped reads (percent)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: ERCC measurement (percent)

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 6 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: TCRb-positive, Foxp3-positive

text: false

text: Mus musculus

text: male

characteristic: title

characteristic: total reads