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characteristic: External Id

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text: public

characteristic: INSDC status

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tag: Namespace:University of Muenster, Medical Faculty, Institute of Bioinformatics

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CADO ES1

characteristic: cell line

text: Ewings sarcoma

characteristic: cell type

text: human

text: Protocols: Ewing Sarcoma cell lines CADO-ES1, was purchased form DSMZ. Ewing sarcoma cell lines were cultivated in RPMI1640 medium, supplemented with 10% (v/v) FCS, 1% Glutamin and 1% of an antibiotic cocktail containing penicillin and streptomycin. All Ewing sarcoma cell lines were maintained on cell culture plastic coated with collagen (0,5mg/ml). Culture media was supplemented by 1% of an antibiotic cocktail containing penicillin and streptomycin and incubated at 37°C and 5%CO2 in a humidified atmosphere. Routine microbiological analysis was done using a PCR Mycoplasma Detection Kit according to suggested protocols (Applied Biosystems, Darmstadt, Germany). The extraction of nucleic acids from cell cultures was performed by the use of the DNeasy Kit (Qiagen) according to the supplier's recommendations. For DNA library preparation 500 - 5000ng DNA were used. ERCCs as an additional internal quality control were added to the libraries. We followed exactly the Thermo-Fischer/Life-Technology(ABI) protocols for the SOLID-5500XL Technology for DNAseq. Most of the procedures were run in a core lab facility of Life-Technology as a commercial service.

characteristic: description

text: Ewing sarcoma

characteristic: disease

text: EWS-ERG

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text: pleural fluid

characteristic: metastatic site

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: genome


status: PUBLIC

tag: attribute

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text: Michigan State University

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tag: Namespace:Michigan State University

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text: Human Postmortem Microbiome - Ecology of 188 Cases

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text: urban

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text: Rectum

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text: USA

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

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text: female

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text: human-associated

characteristic: human associated environmental package

text: mimarks-survey

characteristic: investigation type

text: invitrogen PureLink� Genomic DNA Mini Kit

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text: Homo sapiens

text: Human Postmortem Microbiome

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text: Cotton Tipped Swab

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unit: °C

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text: Illumina MiSeq

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text: USA

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text: Cotton Tipped Swab

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text: Nose

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text: USA

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text: urban

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text: Eyes

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text: USA

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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characteristic: ENA-CHECKLIST

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text: mimarks-survey

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text: Homo sapiens

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text: urban

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text: USA

text: 83� 6'19.55W

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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text: mimarks-survey

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text: Nose

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text: USA

text: 83� 9'36.93W

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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text: Illumina MiSeq

text: 16S rRNA


characteristic: ENA-CHECKLIST

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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text: 16S rRNA


characteristic: ENA-CHECKLIST

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text: 83� 8'50.99W

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text: Cotton Tipped Swab

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text: urban

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text: Nose

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characteristic: estimated postmortem interval

text: USA

text: 83� 3'19.47W

text: male

text: human-associated

text: mimarks-survey

text: Homo sapiens

characteristic: organism

text: Human Postmortem Microbiome

text: Cotton Tipped Swab

text: Homo sapiens

text: Illumina MiSeq

text: 16S rRNA


characteristic: ENA-CHECKLIST

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text: Michigan State University

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characteristic: geographic location (country and/or sea)

text: 42� 6'41.60N

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text: Cotton Tipped Swab

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characteristic: ENA-CHECKLIST

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text: Cotton Tipped Swab

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text: mimarks-survey

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text: Human Postmortem Microbiome

text: Cotton Tipped Swab

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characteristic: ENA-CHECKLIST

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text: Michigan State University

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text: USA

characteristic: geographic location (country and/or sea)

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text: mimarks-survey

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text: Human Postmortem Microbiome

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