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text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x467

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.674571 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x468

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 11.674571 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x469

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 11.674571 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x470

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.674571 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x471

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 8.965429 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x472

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 8.965429 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x473

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 8.965429 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x474

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 8.977 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x475

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 8.977 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x476

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

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text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x477

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 8.163714 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x478

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 8.163714 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x479

characteristic: title

text: water


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characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 8.163714 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x480

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 11.147375 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x481

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 11.147375 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x482

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.147375 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x483

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 8.956 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x484

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 8.956 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x485

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 8.956 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x486

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 11.496571 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

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text: PE Illumina Miseq 2x487

characteristic: sequencing method

text: water

characteristic: water environmental package


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text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 11.496571 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x488

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.496571 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x489

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 11.496571 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x490

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 12.017429 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x491

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 12.017429 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x492

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 12.017429 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x493

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 12.017429 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x494

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.641857 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x495

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 11.641857 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x496

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 11.641857 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x497

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 11.641857 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x498

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 10.776333 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x499

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 10.776333 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x500

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 10.776333 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x501

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 10.776333 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x502

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 10.088714 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x503

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: common name

text: Pacific Ocean

text: 10.088714 m

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x504

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

text: Pacific Ocean

text: 10.088714 m

characteristic: geographic location (depth)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: organism

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x505

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: collection date

text: seawater metagenome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: 10.088714 m

characteristic: geographic location (latitude)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x506

characteristic: title

text: water


blank.DNA.ae.fastq

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blank.DNA.ae.fastq

characteristic: Submitter Id

text: unidentified

text: unidentified

text: blank.DNA.ae.fastq


blank.extract.DNA.dura.fastq

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: blank.extract.DNA.dura.fastq

text: unidentified

characteristic: organism

text: unidentified

text: blank.extract.DNA.dura.fastq


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: unidentified

text: unidentified


blank.PCR.noRT.dura.fastq

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: blank.PCR.noRT.dura.fastq

text: unidentified

text: unidentified

text: blank.PCR.noRT.dura.fastq


mock.even.DNA.ae.fastq

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: mock.even.DNA.ae.fastq

text: synthetic metagenome

text: synthetic metagenome

text: mock.even.DNA.ae.fastq


text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: synthetic metagenome

text: synthetic metagenome


mock.stag.DNA.ae.fastq

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: mock.stag.DNA.ae.fastq

text: synthetic metagenome

text: synthetic metagenome

text: mock.stag.DNA.ae.fastq


pcr.blank.RNA.ae.fastq

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: pcr.blank.RNA.ae.fastq

text: unidentified

text: unidentified

text: pcr.blank.RNA.ae.fastq


text: TEMASEK LIFE SCIENCES LABORATORY

text: public

tag: Namespace:TEMASEK LIFE SCIENCES LABORATORY

text: barramundi perch

characteristic: common name

text: Brain and kidney samples were collected from the same adult back-crossed individual (ASB-BC8). The genomic DNA from the brain was used for generating a single ~10 kb insert size library which was sequenced using 77 SMRT cells on PacBio RSII using C2-XL chemistry, generating 20.3 Gb of data or a ~30X coverage of the Asian seabass genome. The genomic DNA from the kidney was used for generating a ~20 kb insert size library which was sequenced using 105 SMRT cells on PacBio RSII using C3-P5 chemistry, generating 42.14 Gb of data or a ~60X coverage of the Asian seabass genome.

characteristic: description

text: Lates calcarifer

text: Lates calcarifer

text: Male

characteristic: sex

text: ASB-BC8 adult back-crossed individual


text: RADBOUD UNIVERSITY NIJMEGEN

text: public

tag: Namespace:RADBOUD UNIVERSITY NIJMEGEN

text: New species of Methylomirabilis

text: Candidatus Methylomirabilis oxyfera

text: Candidatus Methylomirabilis oxyfera

text: New species of Methylomirabilis


text: JHI

text: public

text: barley

text: spring barley, high DP

text: Hordeum vulgare

text: Hordeum vulgare


text: JHI

text: public

text: barley

text: spring barley, low DP

text: Hordeum vulgare

text: Hordeum vulgare


text: JHI

text: public

text: barley

text: winter barley, high DP

text: Hordeum vulgare

text: Hordeum vulgare


text: JHI

text: public

text: barley

text: winter barley, low DP

text: Hordeum vulgare

text: Hordeum vulgare


text: public

tag: Namespace:Pulmonary and Critical Care Medicine, Stanford University School of Medicine

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: lymphoblast

characteristic: cell type

text: human

text: Protocols: Cells were harvested and crosslinked in 1% formaldehyde for 10 minutes at room temperature. Cell pellets were re-suspended in hypotonic buffer (20 mM HEPES, 10 mM KCl, 1mM EDTA, 10% glycerol) supplemented with Pierce Protease Inhibitor Tablets (Thermo Fisher), placed on ice for 10 minutes and centrifuged to remove cytoplasmic supernatant. Chromatin was released from nuclear pellet with mild chemical lysis with Radio-immunoprecipitation assay buffer (RIPA) containing 0.1% SDS and 1% Triton-X-100. Protein-bound chromatin was sheared with mechanical sonication (Branson 250 Sonifier) at 35% output with 20 second pulses for 30 cycles to obtain chromatin fragments of high resolution at 200 â€“ 500 bps. Successful chromatin fragmentation was assayed by reverse cross-linking, and electrophoresis through 0.8% agarose gel to confirm enrichment of small chromatin fragments at the target range. K562 erythroleukemia cells were grown in RPMI1640 media supplemented with 10% fetal bovine serum and Antibiotic-Antimycolic 100X (Life Technologies). Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with with monoclonal antibody to NF90 (BD mAB DRBP76 #612155). Libraries were prepared according to Bravo automatic library preparation.

text: chronic myelogenous leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: Homo sapiens

text: female


E-MTAB-6028:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6028:Sample 1

tag: Namespace:Institute of Experimental Immunology, University of Zurich

unit: day

characteristic: age

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: Protocols: Mice were sacrificed by CO2 inhalation and intracardially perfused with approximately 20 ml PBS. The lung was carefully removed and cut into small pieces using scissors, followed by a tissue digestion in 0.4 mg/ml collagenase type IV (from Clostridium histolyticum) in HBSS (containing Ca2+ / Mg2+) supplemented with 10 % FCS for 45 min at 37C while agitating. Digested tissues were passed through an 18 G syringe and filtered through a 70m cell strainer to obtain a homogeneous cell suspension. After PBS washing and centrifugation at 4C for 5 min at 1500rpm, the cell pellet was resuspended in red blood cell lysis buffer and incubated on ice for 8 mins. After further PBS washing and centrifugation, the cell pellet was resuspended in antibody mix and stained at 4C for 25 mins. After PBS washing and centrifugation, resuspend the cell pellet in FACS buffer (2mM EDTA, 2%FCS in PBS) and ready for FACS sorting. Sorted cells were lysed in RLT plus buffer (Qiagen) supplemented with 1 % mercaptoethanol. RNA isolation was performed using the RNeasy Micro Plus Kit (Qiagen) according to the manufacturers instructions. The quantity and quality of the isolated RNA was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent,Waldbronn, Germany). The TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc,California, USA) was used in the succeeding steps. Briefly, total RNA samples (100 ng) were poly-A selected and then reverse-transcribed into double-stranded cDNA with Actinomycin added during first-strand synthesis. The cDNA samples was fragmented, end-repaired and adenylated before ligation of TruSeq adapters. The adapters contain the index for multiplexing. Fragments containing TruSeq adapters on both ends were selectively enriched with PCR. The quality and quantity of the enriched libraries were validated using Qubit® (1.0) Fluorometer and the Bioanalyzer 2100 (Agilent, Waldbronn, Germany). The product is a smear with an average fragment size of approximately 360 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.

text: normal

text: CD11cCre/+ Tgfbr2fl/fl

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lung

text: Tgfbr2 knockout in CD11c positive cells

characteristic: phenotype

text: Mus musculus

text: mixed

characteristic: strain

text: Sample 1

characteristic: title

type: has member


E-MTAB-6028:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute of Experimental Immunology, University of Zurich

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6028:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: normal

text: CD11cCre/+ Tgfbr2fl/fl

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Tgfbr2 knockout in CD11c positive cells

text: Mus musculus

text: mixed

text: Sample 2


E-MTAB-6028:Sample 3

text: public

text: E-MTAB-6028:Sample 3

text: ArrayExpress

characteristic: broker name

text: alveolar macrophage

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: CD11cCre/+ Tgfbr2fl/fl

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Tgfbr2 knockout in CD11c positive cells

text: Mus musculus

text: mixed

characteristic: sex

text: Sample 3


E-MTAB-6028:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6028:Sample 4

characteristic: age

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: normal

text: CD11cCre/+ Tgfbr2fl/fl

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lung

text: Tgfbr2 knockout in CD11c positive cells

characteristic: phenotype

text: Mus musculus

text: mixed

characteristic: strain

text: Sample 4


E-MTAB-6028:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6028:Sample 5

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: normal

text: Mus musculus

text: Mus musculus

text: lung

text: wild type phenotype

text: Mus musculus

text: mixed

text: Sample 5

characteristic: title


E-MTAB-6028:Sample 6

text: public

text: E-MTAB-6028:Sample 6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: alveolar macrophage

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: wild type phenotype

text: Mus musculus

text: mixed

text: Sample 6


E-MTAB-6028:Sample 7

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6028:Sample 7

characteristic: age

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: normal

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: wild type phenotype

text: Mus musculus

text: mixed

characteristic: sex

text: Sample 7


E-MTAB-6028:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6028:Sample 8

text: ArrayExpress

text: alveolar macrophage

text: house mouse

text: normal

text: Mus musculus

text: Mus musculus

text: lung

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: mixed

characteristic: strain

text: Sample 8


text: Salmonella enterica

characteristic: ArrayExpress-SPECIES

text: MA.SARB67 (Fidelma Boyd)

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Salmonella enterica

characteristic: common name

text: Salmonella enterica

text: Salmonella enterica

characteristic: subject id

characteristic: title


text: Salmonella enterica

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: Salmonella enterica

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA5 (Fidelma Boyd)

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA34 (Fidelma Boyd)

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA69 (Fidelma Boyd)

text: SC

characteristic: INSDC first public

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB35 (Fidelma Boyd)

text: SC

characteristic: INSDC last update

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA28 (Fidelma Boyd)

text: SC

text: public

characteristic: INSDC status

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA8 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA10 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA62 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA19 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB29 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA26 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.ZJ-A

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA25 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA31 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB28 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB57 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB41 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB37 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB56 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA24 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB24 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA70 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB39 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB72 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA40 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB53 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB71 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB34 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA72 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA20 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA18 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB23 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB21 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA1 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB31 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA71 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA36 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA6 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB30 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.ZJ-C

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB62 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB50 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA17 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA3 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB40 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB36 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB22 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA21 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB59 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.ZJ-G

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA14 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB27 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA11 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARB60 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA9 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA32 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.SARA37 (Fidelma Boyd)

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.9 PNS-29

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.40 BG-61

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.46, PAR-87

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.75 KAK-3

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.82 KAK-S

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.11 KAK-S

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.41 GOUM-11

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.47 PAR-88

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.67 PAR-41

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.83 KAK-3

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.13 KOB-M

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.32 DEN-M4

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.49 SUB-5

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Salmonella enterica

text: MA.68 YAT-7

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica