characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000706) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 100 minutes, starting at 12:30 and ending at 14:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000706


status: PUBLIC

tag: attribute

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

unit: DD

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

unit: practical salinity unit

characteristic: Salinity Sensor

tag: Namespace:PANGAEA Data Publisher for Earth and Environmental Science

characteristic: Submitter Id

unit: m

characteristic: depth

text: This sample (EMOSE_N010000707) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 141 minutes, starting at 12:55 and ending at 15:16 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

unit: ºC

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000707

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000708) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 90 minutes, starting at 14:25 and ending at 15:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000708


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000709) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 120 minutes, starting at 15:35 and ending at 17:35 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000709

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000710) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 100 minutes, starting at 16:20 and ending at 18:00 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000710


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000717) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 114 minutes, starting at 17:49 and ending at 19:43 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000717

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000712) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 85 minutes, starting at 18:25 and ending at 19:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000712


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000713) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000715,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 131 minutes, starting at 19:59 and ending at 22:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000713

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N010000715) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000716,EMOSE_N010000713,EMOSE_N010000704

text: Westerlies Biome

text: water

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 93 minutes, starting at 20:12 and ending at 21:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000715


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000716) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W>20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000712,EMOSE_N010000717,EMOSE_N010000710,EMOSE_N010000709,EMOSE_N010000708,EMOSE_N010000707,EMOSE_N010000706,EMOSE_N010000705,EMOSE_N010000715,EMOSE_N010000713,EMOSE_N010000704

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W>20, the sample material was not pre-filtered and concentrated on 20-micrometres, using a 47-mm-diameter nylon mexh filter. A volume of 100 Litres was filtered and the 47-mm-diameter nylon mexh filter was packaged in a sterile 2-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 70 minutes, starting at 22:15 and ending at 23:25 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W>20_N010000716

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: This sample (EMOSE_N020000684) was created in silico using samples EMOSE_N010000684, EMOSE_N010000686, EMOSE_N010000688, EMOSE_N010000692, EMOSE_N010000690, EMOSE_N010000694, EMOSE_N030000828, EMOSE_N010000698, EMOSE_N010000700 and EMOSE_N010000702. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 1000 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 116 minutes, starting at 10:24 and ending at 12:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N020000684


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000684) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 116 minutes, starting at 10:24 and ending at 12:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000684

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000686) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 138 minutes, starting at 10:32 and ending at 12:50 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000686


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000688) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 105 minutes, starting at 12:30 and ending at 14:15 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000688

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000692) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 145 minutes, starting at 12:55 and ending at 15:20 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000692


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000690) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 105 minutes, starting at 14:25 and ending at 16:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000690

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000694) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 120 minutes, starting at 15:35 and ending at 17:35 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000694


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N030000828) was created in silico using samples EMOSE_N010000828, EMOSE_N010000696 and EMOSE_N010000832. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. Filters from the pooled samples were combined before extraction. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 139 minutes, starting at 19:46 and ending at 22:05 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N030000828

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000828) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000696 and EMOSE_N010000832

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 40 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 139 minutes, starting at 19:46 and ending at 22:05 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000828


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000696) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000828 and EMOSE_N010000832

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 50 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 100 minutes, starting at 16:20 and ending at 18:00 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000696

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000832) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000828 and EMOSE_N010000696

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 100 minutes, starting at 16:20 and ending at 18:00 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000832


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000698) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 120 minutes, starting at 17:49 and ending at 19:49 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000698

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000700) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000702,EMOSE_N010000684

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 100 minutes, starting at 18:25 and ending at 20:05 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000700


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000702) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N030000829,EMOSE_N010000684

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 131 minutes, starting at 19:59 and ending at 22:10 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000702

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N030000829) was created in silico using samples EMOSE_N010000829 and EMOSE_N010000831. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. Filters from the pooled samples were combined before extraction. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000700,EMOSE_N010000698,EMOSE_N010000694,EMOSE_N010000692,EMOSE_N010000690,EMOSE_N010000688,EMOSE_N010000686,EMOSE_N030000828,EMOSE_N010000702,EMOSE_N010000684

text: Westerlies Biome

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 153 minutes, starting at 20:12 and ending at 22:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N030000829


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N010000829) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000831

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: further details

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 50 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 153 minutes, starting at 20:12 and ending at 22:45 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000829

characteristic: title


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: This sample (EMOSE_N010000831) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has aliquot sample(s): EMOSE_N010000829

text: Westerlies Biome

text: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [MS] Mediterranean Sea (MRGID:1905)

characteristic: marine region

text: marine metagenome

text: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods

characteristic: project name

text: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 50 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 98 minutes, starting at 22:15 and ending at 23:53 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume well pump

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000831


characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: PANGAEA Data Publisher for Earth and Environmental Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (EMOSE_N020000685) was created in silico using samples EMOSE_N010000685, EMOSE_N010000687, EMOSE_N010000689, EMOSE_N010000691, EMOSE_N010000693, EMOSE_N010000695, EMOSE_N010000697, EMOSE_N010000699, EMOSE_N010000701 and EMOSE_N010000703. The latter samples were collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201705310750Z_DAY2_EVENT-PUMP) occurred at position latitudeN=42.486567 and longitudeE=003.1702, on date/time=2017-05-31T07:50Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. The pooled samples were extracted separately and parts of the DNA from each replicate were pooled before sequencing. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): none

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

text: [MS] Mediterranean Sea (MRGID:1905)

text: marine metagenome

characteristic: organism

text: In protocole SEQ-(100L-on-membrane)_W3-20, the sample material was pre-filtered on 20-micrometres, using a 47-mm-diameter nylon mexh filter, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 1000 Litres was filtered and the 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 91 minutes, starting at 10:24 and ending at 11:55 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume well pump

text: This version can be used to provide data discovery services

text: marine metagenome

text: EMOSE_201705310750Z_DAY2_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W3-20_N020000685

characteristic: title