text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RENASANSA'

text: Triticum aestivum

text: Triticum aestivum


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TGAC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: bread wheat

characteristic: common name

text: Bread wheat line 'TRINTELLA'

characteristic: description

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

type: has member


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'PIKO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'WEEBIL'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CHARGER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SHAMROCK'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'BOREONOS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'BUSTER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ACCESS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CLAIRE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CONSORT'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ESCORIAL'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ETOILE-DE-CHOISY'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'EXTREM'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'FANAL'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'FLAME'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'FLORIDA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HAVEN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HOBBIT'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HOLDFAST'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MARIS-HUNTSMAN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HUSTLER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ISTABRAQ'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'KAVKAZ'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'LEDA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'LONGBOW'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MALACCA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MARIS-WIDGEON'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MENDEL'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MIRAS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MIRONOVSKA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MUCK'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MULTIWEISS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SHANGO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'NORMAN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'OBELISK'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ORLANDO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'PALUR'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RIALTO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RIBAND'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SOISSONS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: rape

text: Doubled haploid line TN011 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN016 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN017 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN018 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN019 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN029 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN032 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN034 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN039 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN040 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN045 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN047 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN060 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN064 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN066 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN075 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN078 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN081 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN082 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN087 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN089 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN093 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN097 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN098 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN114 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN135 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN142 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN144 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN152 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN157 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN159 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN160 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN173 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN175 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN178 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN181 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN190 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN192 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Doubled haploid line TN198 from Tapidor x Ningyou 7 mapping population

text: Brassica napus

text: Brassica napus


text: EMBL

text: public

text: ArrayExpress

characteristic: broker name

text: multipotent progenitor cell, fraction 1

characteristic: cell type

text: house mouse

text: Protocols: Bone marrow was isolated from hindlegs (femur, tibia), hips (ilium) and backbone (vertebra). Muscle, connective tissue and spinal cord were removed, bones were crushed in RPMI/ 2 percent FBS (Gibco, Carlsbad, CA) using mortar and pestle. Single cell-suspensions were made by flushing through a 40 um filter mesh. Cell numbers and viability were determined using a ViCell Counter (Beckman Coulter, Brea, CA). To deplete lineage-positive cells, total bone marrow was stained 30 minutes with a combination of the following monoclonal rat antibodies directed against mature cell specific lineage markers: anti-CD4 (clone GK1.5), anti-CD8a (53.6.7), anti-CD11b (M1/70), anti-B220 (RA3.6B2), anti-GR1 (RB6.8C5) and anti-TER119 (Ter119). Labeled cells were incubated for 20 minutes with polyclonal sheep anti-rat IgG coated magnetic Dynabeads (Invitrogen, Hercules, CA) at a ratio 2:1 beads to cell and depleted using a magnet, enriching for the lineage-negative (Lin-neg) cell fraction. Beads were washed twice with RPMI/ 2 percent FBS to harvest residual cell fractions. Centrifugation steps were carried out at 1,500 rpm and 4 C for 5 minutes (5810r, Eppendorf, Hamburg, Germany). To specify hematopoietic stem and progenitor cells, the Linneg fraction was stained 30 minutes at fixed cell density using the following rat monoclonal fluorochrome-coupled antibodies: anti-Streptavidin, phycoerythrobilin-cyanine dye 7 (PE-Cy7) conjugated; anti-CD117/c-Kit (2B8)-APC-eFl780; anti-Ly6a/Sca-1 (E13-161.7)-APC; anti-CD34 (RAM34)-FITC; anti-CD135 (A2F10)-PE; anti-CD150 (TC15-12F12.2)-PE-Cy5; anti-CD48 (HM-48-1)-PB. Monoclonal antibody conjugates were purchased from eBioscience (San Diego, CA) or BioLegend (San Diego, CA). All antibodies were titrated prior to use. Cell sorting was performed on a FACS Aria I or FACS Aria II (Becton Dickinson, San Jose, CA) at the DKFZ Flow Cytometry Service Unit, Heidelberg, Germany. The following sort parameters were used: 70 um nozzle; 10,000 evt/s.; 70 psi. HSC, MPP1, MPP2, MPP3 and MPP4 were obtained by sorting as described (Wilson et al. 2008, Hematopoietic Stem Cells Reversibly Switch from Dormancy to Self-Renewal during Homeostasis and Repair, http://dx.doi.org/10.1016/j.cell.2008.10.048), respectively, in biological triplicate. Sorted cells were collected into ice-cold RNA lysis buffer (ARCTURUS(R) PicoPure(R) RNA Isolation Kit (Life Technologies, Invitrogen)) and stored at -80 C until further usage. Of note, to determine sample's purity we re-sorted a fraction of each population (each population showed a purity greater than 95 percent). Total RNA isolation was performed from the indicated populations using ARCTURUS(R) PicoPure(R) RNA Isolation Kit (Life Technologies, Invitrogen) according to the manufacturer's instructions. DNAse treatment was performed using RNAse-free DNAse Set (Qiagen). Total RNA was used for quality controls and for normalization of starting material. cDNA-libraries were generated with 10 ng of total RNA using the SMARTer(TM) Ultra Low RNA Kit for Illumina Sequencing (Clontech) according to the manufacturer's indications. Of note, twelve cycles were used for the amplification of cDNA respectively. Paired-end adaptors were applied to each population.

text: wild type

characteristic: genotype

text: Mus musculus

text: mus musculus

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL

text: public

text: ArrayExpress

text: multipotent progenitor cell, fraction 3

text: house mouse

text: wild type

text: Mus musculus

text: mus musculus

text: Mus musculus

text: fresh specimen


characteristic: External Id

text: EMBL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: hematopoietic stem cells

text: house mouse

text: wild type

text: Mus musculus

text: mus musculus

text: Mus musculus

text: fresh specimen


text: EMBL

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: multipotent progenitor cell, fraction 4

text: house mouse

text: wild type

text: Mus musculus

text: mus musculus

text: Mus musculus

text: fresh specimen


text: EMBL

text: public

characteristic: Submitter Id

text: ArrayExpress

text: multipotent progenitor cell, fraction 2

text: house mouse

characteristic: common name

characteristic: description

text: wild type

text: Mus musculus

text: mus musculus

text: Mus musculus

text: fresh specimen


text: public

tag: Namespace:Institute for Molecular Bioscience, University of Queensland

text: 26 year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: pre-extracted RNA was sourced commercially from BioChain Ribosomal depletion and RNA library construction was performed using Truseq Stranded Total RNA kit with Ribo-Zero Human/Mouse/Rat Set A (Illumina, Cat. #RS-122-2201).1ug of total RNA used as starting material. The library preparations were performed according to the manufacturer's protocol (Truseq Stranded Total RNA Sample Prep Guide Pages 58-87) with minor modifications to the fragmentation and the number of PCR cycles (from the recommended 15 down to 8 in order to increase library fragment complexity). After the addition of elute, fragment and high mix to the ribosomal RNA-depleted RNA, samples were incubated on ice and followed immediately by first strand synthesis. The DNA fragments were amplified and the cleaned libraries quantified using high sensitivity Bioanalyzer.

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute for Molecular Bioscience, University of Queensland

text: public

text: 30 year

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

characteristic: title


E-MTAB-2271:B16-F10 cell line rep2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TRON-MAINZ

characteristic: INSDC center name

text: public

text: E-MTAB-2271:B16-F10 cell line rep2

text: ArrayExpress

text: melanoma cell

characteristic: cell type

text: house mouse

text: Protocols: B16F10 melanoma cell line was purchased in 2010 from the American Type Culture Collection (product: ATCC CRL-6475, lot number: 58078645). Early (third and fourth) passages of cells were used for tumor experiments. Reauthentification of cells has not been done since receipt. RNA from B16F10 cells was extracted in triplicate. Using 5 ug of total RNA, barcoded mRNA-seq cDNA libraries were prepared in triplicate using a modified Illumina mRNA-seq protocol.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

text: B16-F10 cell line rep2


E-MTAB-2271:B16-F10 cell line rep1

text: TRON-MAINZ

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-2271:B16-F10 cell line rep1

characteristic: Submitter Id

text: ArrayExpress

text: melanoma cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

text: B16-F10 cell line rep1


E-MTAB-2271:B16-F10 cell line rep3

text: TRON-MAINZ

text: public

text: E-MTAB-2271:B16-F10 cell line rep3

text: ArrayExpress

characteristic: broker name

text: melanoma cell

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

text: B16-F10 cell line rep3


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: iPSC

characteristic: sample description

text: Mus musculus


text: somatic cells

characteristic: ArrayExpress-OrganismPart

text: female

text: Haemonchus contortus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: single adult female, ovaries dissected out

text: Haemonchus contortus

characteristic: strain


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Alberta and Livestock Gentec

text: ArrayExpress

text: low faecal Salmonella shedding

characteristic: clinical information

text: pig

text: Protocols: Samples used in this study were selected from Salmonella negative piglets (crossbred or Yorkshire sows bred to boars from different breeds) belonging to two populations of 40 and 77 individuals each and raised in climate-controlled, fully enclosed isolation facilities at the USDA-ARS-National Animal Disease Center (NADC) in Ames, IA under identical management conditions. At 7 weeks of age, these Salmonella negative pigs received intranasal inoculation of 109 colony-forming units (cfu) of nalidixic acid resistant Salmonella enterica serovar Typhimurium, ST _4232. Approximately 2.3 ml of whole blood was collected from the jugular vein into PAXgene Blood RNA tubes (processed according to manufacturers instructions) from each individual just prior to infection (day 0) and at days 2, 7, 14, and 20 post-inoculation. Blood samples collected at day 0 and day 2 from 16 selected pigs identified as low shedding (n=8) and persistent shedding (n=8) based on extremes of Salmonella shedding levels post innoculation were used for miRNA extraction Total RNA was prepared from 4.5-9.0 ml of solution from the PAXgene Blood RNA tubes. The DNA was removed by in-solution DNase I digestion and RNeasy mini elute kit cleanup as recommended by QIAGEN. PCR assay without reverse transcription was used to confirm that the RNA samples were DNA-free. The quantity and quality of the RNA were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and Nanodrop 2000 (Thermo Scientific, Wilmington, DE). A globin reduction protocol developed in-house (Choi et al.; manuscript under preperation) using porcine specific oligonucleotides was performed. Briefly, 3 ug of denatured total RNA was hybridized in a thermal cycler at 70 C for 2 min with the 10X GR oligonucleotides mix in hybridization buffer (100 mM Tris-HCL, pH 7.6; 200 mM KCl) at 70 C for 5 min, then cooled to 4 C. RNA-DNA hybrids were digested with 2 U RNase H (Ambion) in the reaction buffer (100 mM Tris_HCl, pH 7.6, 20 mM MgCl2, 0.1 mM DTT, Superase-in) at 37 C for 10 min and cooled to 4 C. The reaction was stopped by addition of 0.5 M EDTA. The globin depleted RNA was immediately purified with the RNeasy MinElute cleanup kit (Qiagen, Toronto, Canada, Cat. No.: 74204) according to manufacturers instruction. RNA quality of the globin depleted samples was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, USA). Total RNA (1.5 ug for each sample) was used to construct miRNA libraries using the TruSeq Small RNA Sample Preparation Kit (Illumina, San Diego, CA) according to the manufacturers instructions. Library quality for miRNA libraries was determined using the High Sensitivity DNA Chip. qRT-PCR was then performed for library quantification using the StepOneTM Real-Time PCR System (Applied Biosystems, Carlsbad, CA) with the KAPA SYBR Fast ABI Prism qPCR kit (Kapa Biosystems, Woburn, MA). The individual libraries were adjusted to 2 nM concentrations and pooled before denaturation and dilution according to Illumina's instructions. The diluted libraries (8 - 10 pM) were loaded on a cBot (Illumina) for cluster generation using the TruSeq_ SR Cluster Kit v3 (50 cycles, Illumina).

text: Sus scrofa

text: Sus scrofa

text: whole blood

characteristic: organism part

text: Sus scrofa

text: frozen specimen

characteristic: specimen with known storage state

characteristic: title


text: University of Alberta and Livestock Gentec

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: persistent faecal Salmonella shedding

text: pig

characteristic: common name

characteristic: description

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: low faecal Salmonella shedding

text: pig

text: Sus scrofa

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen


characteristic: External Id

text: GSC

characteristic: INSDC center name

text: public

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_m-RNA Feuilles

text: GSC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: sample_ATW_4565_m-RNA Feuilles

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_m-RNA Tiges

text: GSC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: sample_ATW_4565_m-RNA Tiges

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_m-RNA Epis

text: GSC

text: public

text: sample_ATW_4565_m-RNA Epis

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_G50F1 - feuilles

text: GSC

text: public

text: sample_ATW_4565_G50F1 - feuilles

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_m-RNA Racine

text: GSC

text: public

text: sample_ATW_4565_m-RNA Racine

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_G700G1 - grain

text: GSC

text: public

text: sample_ATW_4565_G700G1 - grain

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_m-RNA Graines

text: GSC

text: public

text: sample_ATW_4565_m-RNA Graines

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


sample_ATW_4565_E1T1 - stade epi

text: GSC

text: public

text: sample_ATW_4565_E1T1 - stade epi

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum


text: MMRI

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: TGAC

text: public

text: TGAC

text: durum wheat

text: Wheat CS42 CSS TrD

text: Triticum turgidum subsp. durum

text: Triticum turgidum subsp. durum

text: Wheat CS42 CSS TrD


text: K-State IGF

text: public

tag: Namespace:K-State IGF

text: TGAC

text: bread wheat

text: Wheat CS42 CSS 3AL flow-sorted DNA

text: Triticum aestivum

text: Triticum aestivum

text: Wheat CS42 CSS 3AL flow-sorted DNA


text: K-State IGF

text: public

text: TGAC

text: bread wheat

text: Wheat CS42 CSS 3AS flow-sorted DNA

text: Triticum aestivum

text: Triticum aestivum

text: Wheat CS42 CSS 3AS flow-sorted DNA


name: E-MTAB-2266:E15.5 Ngn3^GFP/GFP embryos 1

text: University of Pennsylvania

text: public

tag: Namespace:University of Pennsylvania

text: embryonic day 15.5

characteristic: age

text: ArrayExpress

text: neurogenin-3-lacking pancreatic progenitor cell

characteristic: cell type

text: house mouse

text: Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).

text: embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain

characteristic: strain

text: E15.5 Ngn3^GFP/GFP embryos 1


E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 2

text: University of Pennsylvania

text: public

text: E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 2

characteristic: Submitter Id

text: embryonic day 15.5

text: ArrayExpress

characteristic: broker name

text: neurogenin-3-expressing endocrine progenitor cell

text: house mouse

text: embryo stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: E15.5 Ngn3^GFP/+ embryos 2

characteristic: title


E-MTAB-2266:P60 MIP-GFP mice 3

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P60 MIP-GFP mice 3

text: postnatal day 60

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

characteristic: common name

text: Protocols: Postnatal pancreata were partially dissociated. Islets were hand picked and further dissociated into single cells for FACS-based beta cell purification. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).

characteristic: description

text: post-juvenile adult stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P60 MIP-GFP mice 3


E-MTAB-2266:P4 MIP-GFP mice 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P4 MIP-GFP mice 3

text: postnatal day 4

text: ArrayExpress

text: insulin-expressing immature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P4 MIP-GFP mice 3


E-MTAB-2266:P4 MIP-GFP mice 1

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2266:P4 MIP-GFP mice 1

text: postnatal day 4

text: ArrayExpress

text: insulin-expressing immature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P4 MIP-GFP mice 1


name: E-MTAB-2266:E15.5 Ngn3^GFP/GFP embryos 3

text: University of Pennsylvania

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: embryonic day 15.5

text: ArrayExpress

text: house mouse

text: embryo stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: E15.5 Ngn3^GFP/GFP embryos 3


E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 1

text: University of Pennsylvania

text: public

text: E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 1

text: embryonic day 15.5

characteristic: age

text: ArrayExpress

characteristic: cell type

text: house mouse

text: embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: E15.5 Ngn3^GFP/+ embryos 1


E-MTAB-2266:P1 MIP-GFP mice 1

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P1 MIP-GFP mice 1

characteristic: Submitter Id

text: postnatal day 1

text: ArrayExpress

characteristic: broker name

text: insulin-expressing immature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

characteristic: strain

text: P1 MIP-GFP mice 1


name: E-MTAB-2266:E15.5 Ngn3^GFP/GFP embryos 2

text: University of Pennsylvania

text: public

text: embryonic day 15.5

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: E15.5 Ngn3^GFP/GFP embryos 2

characteristic: title


E-MTAB-2266:P60 MIP-GFP mice 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P60 MIP-GFP mice 2

text: postnatal day 60

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

text: post-juvenile adult stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P60 MIP-GFP mice 2


E-MTAB-2266:P12 MIP-GFP mice 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-2266:P12 MIP-GFP mice 3

text: postnatal day 12

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P12 MIP-GFP mice 3


E-MTAB-2266:P4 MIP-GFP mice 2

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2266:P4 MIP-GFP mice 2

text: postnatal day 4

text: ArrayExpress

text: insulin-expressing immature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P4 MIP-GFP mice 2


E-MTAB-2266:P1 MIP-GFP mice 3

text: University of Pennsylvania

text: public

characteristic: SRA accession

text: E-MTAB-2266:P1 MIP-GFP mice 3

text: postnatal day 1

characteristic: age

text: ArrayExpress

text: insulin-expressing immature beta cell

characteristic: cell type

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P1 MIP-GFP mice 3


E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 3

text: University of Pennsylvania

text: public

text: E-MTAB-2266:E15.5 Ngn3^GFP/+ embryos 3

characteristic: Submitter Id

text: embryonic day 15.5

text: ArrayExpress

characteristic: broker name

text: house mouse

text: embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: E15.5 Ngn3^GFP/+ embryos 3


E-MTAB-2266:P1 MIP-GFP mice 2

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P1 MIP-GFP mice 2

text: postnatal day 1

text: ArrayExpress

text: insulin-expressing immature beta cell

text: house mouse

characteristic: common name

characteristic: description

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

characteristic: strain

text: P1 MIP-GFP mice 2


E-MTAB-2266:P12 MIP-GFP mice 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P12 MIP-GFP mice 2

text: postnatal day 12

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P12 MIP-GFP mice 2

characteristic: title


E-MTAB-2266:P60 MIP-GFP mice 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

text: public

text: E-MTAB-2266:P60 MIP-GFP mice 1

text: postnatal day 60

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

text: post-juvenile adult stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P60 MIP-GFP mice 1


E-MTAB-2266:P12 MIP-GFP mice 1

text: University of Pennsylvania

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-2266:P12 MIP-GFP mice 1

text: postnatal day 12

text: ArrayExpress

text: insulin-expressing mature beta cell

text: house mouse

text: juvenile stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain

text: P12 MIP-GFP mice 1


text: Mouse

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: Mus musculus


text: University of Manchester

text: public

tag: Namespace:University of Manchester

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: Rex1GFPd2 mouse ES cells were maintained in NDiff N2B27 media in the presence of CHIR99021 and PD0325901 as described in Yang et al., 2012. For time series of differentiation, the media containing inhibitor was removed and replaced with NDiff N2B27 media as time indicated. ES cells were cross-linked as described in Lee et al., 2006. Yang SH, Kalkan T, Morrisroe C, Smith A, Sharrocks AD. (2012) PLoS Genet. 2012;8(12):e1003112. A genome-wide RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during embryonic stem cell differentiation. Lee TI, Johnstone SE, Young RA. (2006) Nat Protoc. 1(2):729-48. Chromatin immunoprecipitation and microarray-based analysis of protein location. FAIRE-seq was performed as described in Simon et al., 2012, with the following modifications. The crosslinked material was sheared by water bath sonication using bio-rupter for 17 mins, at high level, 30 secs on/ 30 secs off, 4 C. The size of sheared DNA fragments was between 100 bp to 300 bp, with median size around 200 bp. Simon JM, Giresi PG, Davis IJ, Lieb JD. (2012) Nat Protoc. 7(2):256-67. Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex


E-MTAB-2292:CT26 cell line rep2

characteristic: ENA-CHECKLIST

text: tron-mainz

text: public

text: E-MTAB-2292:CT26 cell line rep2

text: ArrayExpress

text: colorectal carcinoma cell

text: house mouse

characteristic: common name

text: Protocols: CT26 colon carcinoma cell line was purchased in 2010 from the American Type Culture Collection (Product: ATCC CRL-2638, Lot Number: 58494154). Early (3rd, 4th) passages of cells were used for tumor experiments. Cells were routinely tested for mycoplasma. RNA from bulk CT26.WT cells was extracted in triplicate using a combination of Trizol (Invitrogen) and Qiagen RNeasy Micro kit. Barcoded mRNA-seq cDNA libraries were prepared in triplicate, from 5 ug of total RNA (modified Illumina mRNA-seq protocol) mRNA was isolated from total RNA using Seramag Oligo(dT) magnetic beads (Thermo Scientific) and fragmented using divalent cations and heat. Resulting fragments (160-220 bp) were converted to cDNA using random primers and SuperScriptII (Invitrogen) followed by second strand synthesis using DNA polymerase I and RNaseH. cDNA was end repaired, 5' phosphorylated and 3' adenylated according to the manufacturer's instructions. 3' single T-overhang Illumina multiplex specific adapters were ligated with T4 DNA ligase using a 10:1 molar ratio of adapter to cDNA insert. cDNA libraries were purified and size selected at 200-220 bp (E-Gel 2% SizeSelect gel, Invitrogen). Enrichment, adding of Illumina six base index and flow cell specific sequences was done by PCR using Phusion DNA polymerase (Finnzymes). All cleanups up to this step were done with 1,8x volume of Agencourt AMPure XP magnetic beads. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined using Agilent's 2100 Bioanalyzer HS DNA assay.

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

text: CT26 cell line rep2


E-MTAB-2292:CT26 cell line rep1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: tron-mainz

text: public

text: E-MTAB-2292:CT26 cell line rep1

text: ArrayExpress

text: colorectal carcinoma cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

text: CT26 cell line rep1

characteristic: title


E-MTAB-2292:CT26 cell line rep3

text: tron-mainz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-2292:CT26 cell line rep3

text: ArrayExpress

text: colorectal carcinoma cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: fresh specimen

text: CT26 cell line rep3


text: University of Oxford

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Oxford

characteristic: Submitter Id

text: ArrayExpress

text: M1 Macrophages

text: house mouse

text: Protocols: For the generation of M1 macrophages differentiated with GM-CSF, bone marrow of wild type C57Bl6 mice was cultured in RPMI-1640 medium (PAA Laboratories) supplemented with recombinant mouse GM-CSF (20 ng/ml; Preprotech). After 8 d, cells were washed with PBS and replated, then stimulated with LPS (100 ng/ml; Alexis Biochemicals). 50, 100 and 300 million GM-CSF derived M1 macrophages were used for each Pol II, RelA and IRF5 ChIP experiment, respectively. Cells were fixed for 10 minutes with 1 % formaldehyde, quenched with 125 mM of Tris pH 7.5 and washed with ice-cold PBS. Nuclear Each lysate was immunoprecipitated with 10 usc_20x_hg19_rCRSchrm.fa.bamg of the following antibodies: IRF5 (Abcam; ab21689), RelA (Santa Cruz; sc-372) and PolII (Santa Cruz; sc-899). ChIP was performed for each antibody as described previously (Ghisletti et al. 2010). ChIPped DNA w

text: adult

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: wildtype

characteristic: phenotype

text: Mus musculus

text: fresh specimen

characteristic: strain


characteristic: ENA-CHECKLIST

text: University of Turin

text: public

tag: Namespace:University of Turin

text: ArrayExpress

characteristic: broker name

text: tomato

characteristic: common name

text: Protocols: Solanum lycopersicum cv. Moneymaker tomato plants were grown in greenhouse from March through December under natural light, which provided a photoperiod ranging from 9h to 15 h, temperature from 16 degrees C to 30 degrees C and relative humidity from 55% to 80%. The seedlings were grown in pots with sterile quartz sand (80%) and sterile pumice (20%) for non-inoculated plants or with a mixture of sand (55%), sterile pumice (20%) and Funneliformis mosseae (formerly Glomus mosseae) inoculum (25%) Gerd. and Trappe (BEG 12) purchased from Agrauxine (Bretagne, France, http://www.agrauxine.fr) for inoculated plants. Forty-five plants divided into 3 groups of fifteen plants according to their growth conditions were considered: plants grown with F. mosseae inoculum (MYC) or without inoculum were watered, once a week, with a modified Long-Ashton solution containing a low phosphorus and nitrogen concentration (3.2 uM P, 1mM N). The fertilized control plants (CONT) were grown without inoculum and watered, once a week, with a commercial fertilizer solution (7.6mM P, 13.48mM N). At the end of the experiment, the MYC plant roots were cut and fungal colonization was assessed according to the Trouvelot system using MYCOCALC software. Fruits were harvested from the CONT and MYC plants when they reached the turning stage. RNA was extracted using the pine tree-method, with the addition of 1% PVPP to the extraction buffer. For each growth condition, we used two biological replicates, each one formed by pooled RNAs of three fruits. Ten micrograms of each RNA sample was sent to Fasteris Life Science Co. (Geneva, Switzerland) where the libraries were produced.

text: Solanum lycopersicum

text: Solanum lycopersicum L.

text: fruit

characteristic: organism part

text: Solanum lycopersicum

text: fresh specimen

text: presence of Funneliformis mosseae fungus

characteristic: stimulus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Turin

text: public

text: ArrayExpress

text: tomato

characteristic: description

text: Solanum lycopersicum

text: Solanum lycopersicum L.

text: fruit

text: Solanum lycopersicum

text: fresh specimen

characteristic: specimen with known storage state

text: no Funneliformis mosseae fungus


name: E-MTAB-2301:Non-inoculated  T. aestivum roots (sample 1)

text: State University of Londrina

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:State University of Londrina

text: ArrayExpress

text: bread wheat

text: Protocols: T. aestivum seeds were surface-sterilized and germinated (in water agar plates) at 30 degrees C for 12 h under darkness. Germinated seedlings were transferred to sterile glass tubes containing 25 mL Hoagland's nutrient solution (two seedlings per tube) and cultivated at 26 degrees C under 14 h light/10 h darkness for 24 h. In each tube were then added 0.25 mL of sterile Hoagland's nutrient solution and incubated for 3 days at 26 degrees C under 14 h light/10 h darkness. RNAqueous (Ambion) SOLiD Total RNA-Seq Kit (barcoded by Transcriptome Multiplexing Kit) (Life Technologies)

text: 4 days old root

text: not colonised by Azospirillum brasilense

characteristic: growth condition

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: root

text: Triticum aestivum

text: fresh specimen

characteristic: title


name: E-MTAB-2301:Non-inoculated  T. aestivum roots (sample 2)

text: State University of Londrina

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: bread wheat

text: 4 days old root

characteristic: developmental stage

text: Triticum aestivum

text: Triticum aestivum

text: root

text: Triticum aestivum

text: fresh specimen


name: E-MTAB-2301:Colonized T. aestivum roots (sample 1)

characteristic: ENA-CHECKLIST

text: State University of Londrina

text: public

text: ArrayExpress

characteristic: broker name

text: bread wheat

characteristic: common name

text: Protocols: T. aestivum seeds were surface-sterilized and germinated (in water agar plates) at 30 degrees C for 12 h under darkness. Germinated seedlings were transferred to sterile glass tubes containing 25 mL Hoagland nutrient solution (two seedlings per tube) and cultivated at 26 degrees C under 14 h light/10 h darkness for 24 h. Each tube were then inoculated with 0.25 mL of A. brasilense solution grown until optical density (600 nm) of 1.0. The inoculated seedlings were incubated for 3 days at 26 degrees C under 14 h light/10 h darkness. RNAqueous (Ambion) SOLiD Total RNA-Seq Kit (barcoded by Transcriptome Multiplexing Kit) (Life Technologies)

text: 4 days old root

text: colonised by Azospirillum brasilense

text: Triticum aestivum

text: Triticum aestivum

text: root

characteristic: organism part

text: Triticum aestivum

text: fresh specimen

text: Colonized T. aestivum roots (sample 1)


name: E-MTAB-2301:Colonized T. aestivum roots (sample 2)

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: State University of Londrina

text: public

text: ArrayExpress

text: bread wheat

text: 4 days old root

text: colonised by Azospirillum brasilense

text: Triticum aestivum

text: Triticum aestivum

text: root

text: Triticum aestivum

text: fresh specimen

text: Colonized T. aestivum roots (sample 2)


text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Embryo DNA

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Tail DNA

text: Mus musculus


text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: mESC

text: Mus musculus


text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TTCCTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TAGTGACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCCTCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TAGTCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TTCCATTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCGAAGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

characteristic: sample description

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TAACGCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed

characteristic: strain


text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TTGGTATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGAACTGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TACTTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCTCACGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCAGGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TAAGTTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCCAGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGTATGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TCATTGAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_40_1_sibling_10-sc-1856275

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 35 clutch 1. A 8 base indexing sequence (TGGCTCAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph40

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TTCCTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TAGTGACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TACAGGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCCTCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TAGTCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCGAAGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TAACGCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TTGGTATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TACTTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCTCACGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCAGGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TAAGTTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCCAGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TGTATGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TCATTGAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 45 clutch 1. A 8 base indexing sequence (TGGCTCAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph45

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TACTAGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TTCAGCTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_46_1_sibling_10-sc-1856321

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_46_1_sibling_11-sc-1856323

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically abnormal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_46_1_sibling_12-sc-1856325

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single morphologically normal embryo from ZMP phenotype 46 clutch 1. A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph46

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m8881_clutch_1_mutant_1-sc-1856326

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 1. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch1_sibling_1-sc-1856327

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embyos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 1. A 8 base indexing sequence (CGATGTTT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_2_mutant_2-sc-1856328

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 2. A 8 base indexing sequence (TTAGGCAT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_2_sibling_2-sc-1856329

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 2. A 8 base indexing sequence (TGACCACT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_3_mutant_3-sc-1856330

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 3. A 8 base indexing sequence (ACAGTGGT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_3_sibling_3-sc-1856331

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 3. A 8 base indexing sequence (GCCAATGT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_4_mutant_4-sc-1856332

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 4. A 8 base indexing sequence (CAGATCTG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_4_sibling_4-sc-1856333

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 4. A 8 base indexing sequence (ACTTGATG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_5_mutant_5-sc-1856334

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 5. A 8 base indexing sequence (GATCAGCG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_5_sibling_5-sc-1856335

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 5. A 8 base indexing sequence (TAGCTTGT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_6_mutant_6-sc-1856336

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos homozygous for tcf7l1a (allele m881 - PMID:11057671) clutch 6. A 8 base indexing sequence (GGCTACAG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: tcf7l1a_m881_clutch_6_sibling_6-sc-1856337

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from embryos wild type for tcf7l1a (allele m881 - PMID:11057671) clutch 6. A 8 base indexing sequence (CTTGTACT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


characteristic: ArrayExpress-CellType

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: WGA DNA from single HCC38 cell

text: Homo sapiens


text: GSC

text: public

characteristic: INSDC status

text: rape

text: Brassica napus

text: Brassica napus


text: public

characteristic: SRA accession

tag: Namespace:Istituto Nazionale Genetica Molecolare - INGM

text: ArrayExpress

characteristic: broker name

text: primary cell

characteristic: cell property

text: CD4 Th2

characteristic: cell type

text: human

text: Protocols: PBMC from healthy donor lymphocyte subsets sorted from total PBMC RNA extraction by miRvana isolation kit Applied Biosystems

characteristic: description

text: differentiated

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD4+ CRTH2+ CXCR3-

characteristic: phenotype

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Istituto Nazionale Genetica Molecolare - INGM

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: ArrayExpress

text: primary cell

text: CD8 Central Memory

text: human

characteristic: common name

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD8+ CCR7+ CD45RA- CD45RO+

text: Homo sapiens

text: fresh specimen


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: primary cell

text: B Naive

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: CD19+ CD5- CD27-

text: Homo sapiens

text: fresh specimen


text: public

text: ArrayExpress

text: primary cell

characteristic: cell property

text: CD8 Naive

characteristic: cell type

text: human

characteristic: description

text: differentiated

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD8+ CCR7+ CD45RA+ CD45RO-

text: Homo sapiens

text: fresh specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: primary cell

text: CD4 Effector Memory

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD4+ CCR7- CD45RA- CD45RO+

characteristic: phenotype

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: ArrayExpress

text: primary cell

text: CD4 Th1

text: human

characteristic: common name

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD4+ CXCR3+

text: Homo sapiens

text: fresh specimen


text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: primary cell

characteristic: cell property

text: CD4 Naive

characteristic: cell type

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: CD4+ CCR7+ CD45RA+ CD45RO-

text: Homo sapiens

text: fresh specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: primary cell

text: CD4 Central Memory

text: human

characteristic: description

text: differentiated

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD4+ CCR7+ CD45RA- CD45RO+

text: Homo sapiens

text: fresh specimen


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: primary cell

text: B Memory

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD19+ CD5- CD27+

characteristic: phenotype

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary cell

text: CD4 Treg

text: human

characteristic: common name

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD4+ CD127- CD25+

text: Homo sapiens

text: fresh specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: broker name

text: primary cell

characteristic: cell property

text: CD4 Th17

characteristic: cell type

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: CD4+ CCR6+ CD161+ CXCR3-

text: Homo sapiens

text: fresh specimen


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: primary cell

text: B CD5

text: human

characteristic: description

text: differentiated

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD19+ CD5+

text: Homo sapiens

text: fresh specimen


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: primary cell

text: CD8 Effector Memory

text: human

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD8+ CCR7- CD45RA- CD45RO+

characteristic: phenotype

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary cell

text: CD4 Th2

text: human

characteristic: common name

text: differentiated

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD4+ CRTH2+ CXCR3-

text: Homo sapiens

text: fresh specimen


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WTSI

text: public

text: dog

text: CTVT tumour

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: host

text: Maningrida, Northern Territory

characteristic: isolation source

text: Canis lupus familiaris

characteristic: organism

text: Canis lupus familiaris

text: CTVT tumour

characteristic: strain

text: CTVT tumour

characteristic: tissue type


text: WTSI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: dog

text: CTVT host dog

characteristic: description

text: Australia

text: Maningrida, Northern Territory

text: Canis lupus familiaris

text: Canis lupus familiaris

text: Female

characteristic: sex

text: Aboriginal camp dog

text: Liver


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WTSI

text: public

characteristic: INSDC status

text: dog

text: CTVT tumour

text: Brazil

text: Franca, Sao Paulo State

text: Canis lupus familiaris

text: Canis lupus familiaris

text: CTVT tumour

text: CTVT tumour

characteristic: title


characteristic: ENA-LAST-UPDATE

text: WTSI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dog

characteristic: common name

text: CTVT host dog

text: Brazil

text: Franca, Sao Paulo State

text: Canis lupus familiaris

text: Canis lupus familiaris

text: Male

text: American cocker spaniel

text: Liver


characteristic: External Id

text: University of Algarve

text: public

tag: Namespace:University of Algarve

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNAse I (Promega) treatment during 30 min at 37C. After, the DNAse was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq

text: embryo stage

characteristic: developmental stage

text: mutagenesis screen

characteristic: genetic modification

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: defective in blastoderm cellularization

characteristic: phenotype

text: Drosophila melanogaster

text: fresh specimen

characteristic: specimen with known storage state


text: University of Algarve

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fruit fly

characteristic: description

text: embryo stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fresh specimen

characteristic: strain


text: Homo sapiens

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: Genomic DNA library

characteristic: sample description

text: Homo sapiens