text: Bone Marrow

characteristic: ArrayExpress-Phenotype

text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


status: PUBLIC

text: Normal

tag: attribute

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


text: EMBL Heidelberg

text: public

tag: Namespace:EMBL Heidelberg

characteristic: batch name

text: ArrayExpress

characteristic: broker name

text: medullary thymic epithelial cell

characteristic: cell type

text: house mouse

text: single cell transcriptome sequencing Protocols: Human mTECs were processed as in Pinto et al, 2013. Mouse mTECs were isolated and purified as described previously (Rattay et al, 2015). The pre-enriched stromal cell fraction, sorted for total mTECs (n = 259 cells) was stained using the following antibodies: anti-CD45-PerCP (clone 30-F11, BD Pharmingen), anti-EpCAM-Alexa647 (G8.8, described by Farr et al ), anti-CDR1-Pacific Blue or anti-Ly51-FITC (CDR1 hybridoma, described by Rouse et al ; clone 6C3, BD Biosciences). For the antigen-specific mTECs Tspan8 (n = 48) and Ceacam1 (n = 30) additional antibodies were added to the antibody mix namely: anti-I-A(b)-FITC (clone AF6-120.1, BD Pharmingen), anti-Tspan8-PE (clone 657909, RandD Systems) and anti-CD66a-PE (i.e., anti-Ceacam1, clone CC1, eBioscience). Dead cells were excluded using propidium iodide (PI) in a final concentration of 0.2 ug/ml. Cells were sorted on BD FACSAria(TM) III cell sorter (BD Biosciences) using the single-cell sorting mode as previously described (Derbinski et al, 2008) We used a modified version of Smrt-seq2. We used 19 cycles of initial PCR amplification and used a ratio of 0.6:1 (instead of a 1:1 ratio) of Ampure XP beads (Beckman Coulter) for the first PCR purification to minimize primer dimer carryover. After the first PCR amplification, cDNA libraries were screened via qPCR (we used a 1:10 dilution of purified cDNA libraries for qPCR reactions) for expression of a mouse housekeeping gene (Ubc), and library size distribution was checked on the Bioanalyzer instrument (Agilent) as reported previously . Only cDNA libraries that passed both quality controls were processed further. We used 100 pg of cDNA for the tagmentation reaction and applied 12 cycles for the final enrichment PCR of adapter-ligated fragments. The last purification step was performed using a 0.8:1 ratio of Ampure SPRIselect beads (Beckman Coulter).

characteristic: description

text: PI(neg)CD45(neg)Ly51(neg)EpCAM(pos)MHCII(high)

characteristic: facs

characteristic: individual

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

characteristic: single cell well quality

characteristic: title

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL Heidelberg

text: public

text: ArrayExpress

text: medullary thymic epithelial cell

text: house mouse

text: PI(neg)CD45(neg)Ly51(neg)EpCAM(pos)MHCII(low)

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


text: EMBL Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: embl

text: ArrayExpress

text: medullary thymic epithelial cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


text: EMBL Heidelberg

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: medullary thymic epithelial cell

text: house mouse

characteristic: common name

text: PI(neg)CD45(neg)CDR1(neg)EpCAM(pos)MHCII(high)Tspan8(pos)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: OK


text: EMBL Heidelberg

text: public

characteristic: batch name

text: ArrayExpress

characteristic: broker name

text: medullary thymic epithelial cell

characteristic: cell type

text: house mouse

characteristic: description

text: PI(neg)CD45(neg)CDR1(neg)EpCAM(pos)MHCII(high)Ceacam1(pos)

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


text: K562 myelogenous leukemia

characteristic: ArrayExpress-CellType

characteristic: ArrayExpress-Immunoprecipitate

text: Normal

characteristic: ArrayExpress-Phenotype

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

text: Homo sapiens

text: K4me3 ChIP DNA

characteristic: sample description

text: Homo sapiens


text: K562 myelogenous leukemia

text: Normal

text: Homo sapiens

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: human

text: Homo sapiens

text: K27ac ChIP DNA

text: Homo sapiens


text: K562 myelogenous leukemia

text: Normal

text: Homo sapiens

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Input DNA

text: Homo sapiens


text: K562 myelogenous leukemia

text: anti-CTCF

text: Normal

text: Homo sapiens

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: CTCF ChIP DNA

text: Homo sapiens


text: MDC

text: public

text: ArrayExpress

characteristic: broker name

text: macrophage

characteristic: cell type

text: house mouse

text: Protocols: Primary bone-marrow-derived murine macrophages were grown in RPMI1640 (GIBCO) supplemented with 10% FCS, 10mM HEPES, 1% Penicillin-Streptomycin, 5µM beta-mercaptoethanol at a density of 40 million cells per 150cm(2) cell culture flask. After attachment for 2h cells were stimulated with 10ng/ml LPS (Sigma-Aldrich) [M(LPS)] for 24h. 40mM NaCl were added where applicable. 40 million macrophages per group were used to isolate nuclei by centrifugation through a dense sucrose cushion. Subsequently, samples were digested with micrococcal nuclease (Sigma-Aldrich) to generate native chromatin templates consisting mainly of mononucleosomes. Abundance of mononucleosomal DNA was confirmed by gel electrophoresis. Chromatin was extracted with RIPA buffer and cellular debris removed by centrifugation. Subsequently, chromatin was precipitated over night at 4°C with anti-H3K4me3 (NEB #9751 S) bound to Dynabeads® Protein A (Invitrogen #10002D). MinElute PCR Purification Kit (QIAGEN) was used to purify immunoprecipitated DNA after Proteinase K (Sigma-Aldrich) digestion at 65°C for 4h. 50ng of purified DNA was used for library preparation with the TruSeq ChIP sample preparation kit (Illumina) according to the manufacturer's instructions.

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: 10ng/ml LPS 24h

characteristic: stimulus

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MDC

text: public

text: ArrayExpress

text: macrophage

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male


characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:University of Pennsylvania

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: According to protocols available from the UPenn Functional Genomics Core website. Antibody kindly provided by Chris Wright. According to protocols available from the UPenn Functional Genomics Core website.

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus

characteristic: strain or line


name: E-MTAB-3357:WT-3 14 embryos (12 litters)

text: Columbia Unversity

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Columbia Unversity

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II.

text: embryonic day 15.5

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: WT-3 14 embryos (12 litters)


name: E-MTAB-3357:WT-2 17 embryos (12 litters)

text: Columbia Unversity

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: embryonic day 15.5

text: Mus musculus

text: Mus musculus

text: Mus musculus

characteristic: strain

text: WT-2 17 embryos (12 litters)

characteristic: title


E-MTAB-3357:WT-1 4 embryos (2 litters)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Columbia Unversity

text: public

text: E-MTAB-3357:WT-1 4 embryos (2 litters)

text: ArrayExpress

text: house mouse

text: embryonic day 15.5

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: WT-1 4 embryos (2 litters)


E-MTAB-3357:MUT-1 1 embryo (2 litters)

text: Columbia Unversity

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-3357:MUT-1 1 embryo (2 litters)

text: ArrayExpress

text: house mouse

text: embryonic day 15.5

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Nkx2.2tm(null)Suss; Neurog3tm1(EGFP)Khk/Mmcd

text: MUT-1 1 embryo (2 litters)


name: E-MTAB-3357:MUT-2 8 embryos (12 litters)

text: Columbia Unversity

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: embryonic day 15.5

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: MUT-2 8 embryos (12 litters)


name: E-MTAB-3357:MUT-3 7 embryos (12 litters)

text: Columbia Unversity

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: embryonic day 15.5

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: MUT-3 7 embryos (12 litters)


text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: SRA accession

text: human

text: Homo sapiens

text: Homo sapiens


CBTL

text: EBI

text: public

text: CBTL

characteristic: Submitter Id

text: ENA

text: bread wheat

characteristic: common name

text: Triticum aestivum

text: Triticum aestivum

text: BioSample entry for WGS set CBTL


CBVK

text: EBI

text: public

text: CBVK

text: ENA

text: Norway spruce

text: Picea abies

characteristic: organism

text: Picea abies

text: BioSample entry for WGS set CBVK


E-MTAB-3360:Sample 2

text: public

text: E-MTAB-3360:Sample 2

tag: Namespace:Sir William Dunn School of Pathology, University of Oxford

text: ArrayExpress

characteristic: broker name

text: HeLa

characteristic: cell line

text: human

text: Protocols: HeLa cells were grown in DMEM medium supplemented with 10% fetal calf serum, 100U/ml penicillin, 100 g/ml streptomycin, 2 mM L-glutamine at 37ºC and 5% CO2 HeLa cells were either untreated (control) or treated with 100 micromolar KM05283 (Maybridge) or 100 micromolar DRB (Sigma) for 30 minutes. 1.5 x 107 HeLa cells were harvested by centrifugation, washed with PBS, and resuspended in HLB (10 mM Tris pH 7.5, 10 mM NaCl, 2.5 mM MgCl2) + 0.5% NP-40 + 2 units (U)/ml SUPERase In (Ambion). After 5 min incubation on ice, nuclei were pelleted through a cushion of HLB + 0.5% NP-40 + 10% sucrose + 2U/ml SUPERase In. Nuclei were resuspended in an equal volume of transcription buffer (10 mM Tris pH 8, 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 20U SUPERase In, 1% Sarkosyl, 500 uM ATP, GTP and Br-UTP, 2 uM CTP). The transcription reaction was carried out at 30°C for 5 min and terminated by centrifugation for 30 seconds (sec). Nuclei were resuspended in HSB (10 mM Tris pH 7.5, 0.5 M NaCl, 10 mM MgCl2) containing 10U of RNase-free DNase and incubated at 30°C for 20 min. Protein was digested by the addition of 200 μg/ml of proteinase K in buffer (20 mM Tris-HCl pH 7.4, 2% SDS, 10 mM EDTA) at 55°C for 1 hour (h). RNA were extracted twice with acid phenol:chloroform, once with chloroform and precipitated with 3 volumes of -20°C ethanol containing 30 mM NaCl and glycogen. The pellet was washed with 75% ethanol before resuspending in 20 l of DEPC-treated water. Base hydrolysis, immunoprecipitation of Br-U RNA and end-repair were performed as described by Core et. al. Science 322, 1845-8 (2008) The run-on library was prepared by the High-Throughput Genomics Group at The Wellcome Trust Centre for Human Genetics. Two untreated controls, KM-treated and DRB-treated samples were multiplexed together and sequenced on a single lane of Illumina HiSeq using a standard 51 nucleotides (nt) paired-end reads protocol.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 2


E-MTAB-3360:Sample 4

text: Sir William Dunn School of Pathology, University of Oxford

text: public

text: E-MTAB-3360:Sample 4

text: ArrayExpress

text: HeLa

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 4


E-MTAB-3360:Sample 1

text: public

text: E-MTAB-3360:Sample 1

text: ArrayExpress

text: HeLa

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 1

characteristic: title


E-MTAB-3360:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-3360:Sample 3

text: ArrayExpress

text: HeLa

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 3


E-MTAB-3353:pool 1 of E17.5 WT embryos

characteristic: ENA-FIRST-PUBLIC

text: Columbia Unversity

text: public

text: E-MTAB-3353:pool 1 of E17.5 WT embryos

characteristic: age

text: ArrayExpress

text: house mouse

text: Black Swiss background Protocols: Remove embryos and place in cold PBS. Dissect out pancreas from each embryo and place in cold 1X DPBS -/-. Use fluorescent dissecting scope to determine which pancreases are EGFP+ and place them in RPMI + 2% FBS until genotypes are determined. After determining genotypes, pool together WT/EGFP+ and Mut/EGFP+ embryos in 2mL eppendorf tubes and add 1 mL of cold 1X DPBS -/-. Use dissecting scissors or tweezers to chop up Pancreatta to about 1 mm pieces. Spin at 2500 rpm for 5-10' and remove supernatant. Resuspend in 1 mL Accumax and add 5 ul (5 mg/ul) DNaseI. Incubate at 37 degrees for 30-35'. Time will vary with number of Pancreatta and age of embryos. Agitate tube every 5-10 minutes by inverting the tube or pipetting up and down. Be sure to use a fresh pipette tip for each genotype and do not let the tissue get stuck in the tip (as the pancreas can be very sticky). After dissociation, add 1 mL RPMI 1640 with 2% FBS and an additional 5 ul of DNaseI. Spin 2500 rpm for 5' at 4 degrees (keep cold). Remove supernatant and add 1 mL 1X DPBS -/- to wash and remove phenol red from RPMI. Spin at 2500 rpm for 5'. Remove supernatant and resuspend in 250 ul/pancreas (up to 1 mL) 1X DPBS -/-. Filter through blue cap falcon filter tube. Add 1 ul DAPI just before going to the sorter. Vortex sample to mix in DAPI. Keep samples on ice. For sorting we used mCherry chanel, meaning that the cells sorted will be Ghrelin-expressing and also Ghrelin descendants. Cell purity > 99%.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Ghrltm1(Cre-eGFP)Suss; B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J

characteristic: strain

text: pool 1 of E17.5 WT embryos


name: E-MTAB-3353:pool 1 of E17.5 of Nkx2.2 KO embryos

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Columbia Unversity

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Ghrltm1(Cre-eGFP)Suss; Nkx2-2tm5(null)Suss; B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J

text: pool 1 of E17.5 of Nkx2.2 KO embryos


name: E-MTAB-3353:pool 2 of E17.5 of Nkx2.2 KO embryos

text: Columbia Unversity

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: pool 2 of E17.5 of Nkx2.2 KO embryos


E-MTAB-3353:pool 3 of E17.5 WT embryos

text: Columbia Unversity

text: public

characteristic: SRA accession

text: E-MTAB-3353:pool 3 of E17.5 WT embryos

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: pool 3 of E17.5 WT embryos


name: E-MTAB-3353:pool 3 of E17.5 of Nkx2.2 KO embryos

text: Columbia Unversity

text: public

text: ArrayExpress

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: pool 3 of E17.5 of Nkx2.2 KO embryos


E-MTAB-3353:pool 2 of E17.5 WT embryos

text: Columbia Unversity

text: public

text: E-MTAB-3353:pool 2 of E17.5 WT embryos

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: pool 2 of E17.5 WT embryos


CBXY

text: EBI

text: public

text: CBXY

text: ENA

text: European seabass

text: Dicentrarchus labrax

text: Dicentrarchus labrax

text: BioSample entry for WGS set CBXY


text: DPSQ

text: public

text: ena-SAMPLE-DPSQ-26-02-2015-13:50:32:567-2

text: axolotl

text: Ambystoma mexicanum

text: Ambystoma mexicanum


text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA for RNA-Seq Lib

characteristic: sample description

text: Mus musculus


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS18 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Head kidney tissue

characteristic: tissue type

text: BS18 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: three-spined stickleback

text: BS23 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS23 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS54 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS54 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS53 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS53 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS65 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS65 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS20 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Head kidney tissue

text: BS20 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS10 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

characteristic: tissue type

text: BS10 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS16 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS16 Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS58b Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS58b Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS54 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS54 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS19 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS19 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS44 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS44 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS1 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS1 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS21 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS21 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS43 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Head kidney tissue

text: BS43 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS58b Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

characteristic: tissue type

text: BS58b Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS22 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS22 Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS3 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS3 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS2 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS2 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS14 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS14 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS55 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS55 River Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS2 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS2 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS6 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS6 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS6 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Head kidney tissue

text: BS6 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS50b Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

characteristic: tissue type

text: BS50b Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS57 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS57 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS22 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS22 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS49 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS49 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS4 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS4 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS15 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS15 River Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS24 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS24 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS11 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS11 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS62 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Head kidney tissue

text: BS62 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS43 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

characteristic: tissue type

text: BS43 River Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS50b Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS50b Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS4 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS4 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS5 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS5 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS66 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS66 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS16 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS16 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS57 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS57 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS17 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS17 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS60 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Spleen tissue

text: BS60 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS48 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

characteristic: tissue type

text: BS48 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS59 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS59 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS47 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS47 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS51 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS51 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS45 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS45 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS13 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS13 River Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS18 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS18 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS23 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS23 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS66 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Spleen tissue

text: BS66 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS7 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

characteristic: tissue type

text: BS7 River Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS49 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS49 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS44 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS44 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS24 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS24 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS10 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS10 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS19 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS19 River Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS12 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS12 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS45 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50�36'10.40N,-127�15'8.30W). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Canada

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS45 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS8 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Head kidney tissue

text: BS8 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS62 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60�14'41.57N,5�54'55.39E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

characteristic: tissue type

text: BS62 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS7 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS7 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS9 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS9 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS17 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS17 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS12 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS12 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS65 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS65 River Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS8 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54�9'21.61N,10�25'48.52E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Spleen tissue

text: BS8 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS59 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS59 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS21 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Spleen tissue

text: BS21 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS55 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60�15'19.33N,5�55'35.68E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

characteristic: tissue type

text: BS55 River Three-spined stickleback

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS3 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Head kidney tissue

text: BS3 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

characteristic: INSDC center name

text: public

text: three-spined stickleback

text: BS15 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS15 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: three-spined stickleback

text: BS14 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54�17'1.92N,9�56'55.71E). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS14 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

characteristic: SRA accession

text: three-spined stickleback

text: BS9 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS9 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: three-spined stickleback

characteristic: common name

text: BS46 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50�36'16.40N,-127�15'42.3W). Head kidney tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Head kidney tissue

text: BS46 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

text: MPI-EVB

text: public

text: three-spined stickleback

text: BS5 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54�12'15.08N,10�33'41.90E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS5 River Three-spined stickleback


text: MPI-EVB

text: public

text: three-spined stickleback

text: BS13 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54�15'49.02N,10�2'24.47E). Spleen tissue was used for RNA extraction using a Qiagen Omniscript RT kit following the manufacturer's protocol.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Spleen tissue

text: BS13 River Three-spined stickleback


text: 2 days

characteristic: ArrayExpress-Age

text: E14 ESC

characteristic: ArrayExpress-CellType

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: ribominus RNA-seq

characteristic: sample description

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Institut de Biologie de l'Ecole Normale Superieure

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: Cells were treated with 0.01% ethanol vehicle or 1 μM dexamethasone for 1 hour. Cells were cross-linked by adding formaldehyde to a final concentration of 1% and subsequent incubation 3 minutes at room temperature before quenching the reaction by adding glycine to a final concentration of 125 mM. Chromatin was sheared with a Bioruptor water bath sonicator (Diagenode) to produce fragments of ∼100-200 bp. Protein G-coupled magnetic beads (dynabeads, Invitrogen) were preincubated for one hour with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4°C. Subsequently, beads were washed 4 times with 10mM Tris-HCl pH 8.0, 1mM EDTA, 500 mM NaCl, 5% Glycerol, 0.1% Sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/μl BSA followed by 4 additional washes with 20mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% NP-40, 0.5% sodium deoxycholate.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: Institut de Biologie de l'Ecole Normale Superieure

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


name: ena-SAMPLE-MPI FOR DEVELOPMENTAL BIOLOGY-01-03-2015-16:06:06:107-2

text: MPI FOR DEVELOPMENTAL BIOLOGY

text: public

tag: Namespace:MPI FOR DEVELOPMENTAL BIOLOGY

text: thale cress

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana


text: Yeast

characteristic: ArrayExpress-Phenotype

text: Saccharomyces cerevisiae

characteristic: ArrayExpress-Species

text: SC

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: sample description

text: Saccharomyces cerevisiae


E-MTAB-3351:GG67 pool of 6 embryos

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Vanderbilt University

text: public

text: E-MTAB-3351:GG67 pool of 6 embryos

tag: Namespace:Vanderbilt University

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: MIP-EGFP mice [B6.Cg-Tg(Ins1-EGFP)1Hara/J] were crossed with CD1 females to obtain embryo or mice of desired ages. The presence of vaginal plug at noon was considered as E0.5. E16.5 pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Cell purity was >98% in all samples.

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

characteristic: strain

text: GG67 pool of 6 embryos


E-MTAB-3351:GG32 2 adult mice

characteristic: External Id

text: Vanderbilt University

characteristic: INSDC center name

text: public

text: E-MTAB-3351:GG32 2 adult mice

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

text: GG32 2 adult mice

characteristic: title


E-MTAB-3351:GG31 2 adult mice

characteristic: ENA-CHECKLIST

text: Vanderbilt University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3351:GG31 2 adult mice

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

text: GG31 2 adult mice


E-MTAB-3351:GG68 pool of 4 embryos

text: Vanderbilt University

text: public

characteristic: SRA accession

text: E-MTAB-3351:GG68 pool of 4 embryos

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

text: GG68 pool of 4 embryos


E-MTAB-3351:GG66 pool of 4 embryos

characteristic: ENA-FIRST-PUBLIC

text: Vanderbilt University

text: public

text: E-MTAB-3351:GG66 pool of 4 embryos

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

text: GG66 pool of 4 embryos


E-MTAB-3351:GG30 2 adult mice

characteristic: ENA-LAST-UPDATE

text: Vanderbilt University

text: public

text: E-MTAB-3351:GG30 2 adult mice

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh

text: GG30 2 adult mice


text: NIMR

text: public

text: ArrayExpress

text: house mouse

text: Protocols: E12.5 mouse embryos manual dissection of ventral midbrain Chromatin Immunoprecipitation using Foxa2 antibody ChIP-Seq DNA Sample Prep Kit

text: embryonic day 12.5

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: ventral midbrain

characteristic: organism part

text: Mus musculus


text: NIMR

text: public

text: ArrayExpress

text: house mouse

text: Protocols: E12.5 mouse embryos manual dissection of ventral midbrain Chromatin Immunoprecipitation using Foxa1 antibody ChIP-Seq DNA Sample Prep Kit

characteristic: description

text: embryonic day 12.5

text: Mus musculus

text: Mus musculus

text: ventral midbrain

text: Mus musculus


characteristic: External Id

text: NIMR

text: public

text: ArrayExpress

text: house mouse

text: Protocols: E12.5 mouse embryos manual dissection of ventral midbrain ChIP-Seq DNA Sample Prep Kit

text: embryonic day 12.5

text: Mus musculus

text: Mus musculus

text: ventral midbrain

text: Mus musculus

text: Input


name: E-MTAB-3352:GG33 pool of 7 embryos (2 litters)

text: Vanderbilt University

characteristic: INSDC center name

text: public

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: Ngn3^EGFP mice [B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mice] were intercrossed. Embryos were isolated at E15.5. EGFP-positive embryos were separated into Ngn3^EGFP/EGFP and Ngn3^EGFP/+ pools (these could be easily recognized due to the EGFP level differences in the two genotypes: Ngn3^EGFP/EGFP homozygous mice have low EGFP in the pancreas, but high expression in the dudenum and nerve tissues; Ngn3^EGFP+ mice have high EGFP expression in the pancreas, but not in the duodenum. Most embryos were verified by PCR-based genotyping). The presence of vaginal plug at noon was considered as E0.5. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Cell purity > 98%.

text: Mus musculus

text: Mus musculus

text: Mus musculus

characteristic: strain

text: GG33 pool of 7 embryos (2 litters)


name: E-MTAB-3352:GG34 pool of 16 embryos (4 litters)

characteristic: ENA-CHECKLIST

text: Vanderbilt University

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: GG34 pool of 16 embryos (4 litters)

characteristic: title


name: E-MTAB-3352:GG36 pool of 3 embryos (2 litters)

text: Vanderbilt University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: GG36 pool of 3 embryos (2 litters)


name: E-MTAB-3352:GG35 pool of 6 embryos (4 litters)

text: Vanderbilt University

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: GG35 pool of 6 embryos (4 litters)


name: E-MTAB-3352:GG60 pool of 23 embryos (8 litters)

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Vanderbilt University

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: GG60 pool of 23 embryos (8 litters)


name: E-MTAB-3352:GG59 pool of 15 embryos (8 litters)

text: Vanderbilt University

text: public

text: ArrayExpress

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: GG59 pool of 15 embryos (8 litters)


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


IBDV genome

text: EBI

characteristic: INSDC center name

text: public

text: IBDV genome

text: ENA

text: Gumboro virus

text: Infectious bursal disease virus

text: Infectious bursal disease virus

text: BioSample entry for Infectious bursal disease virus genome


text: EBI

text: public

text: ENA

text: Japanese medaka

text: Oryzias latipes

text: Oryzias latipes

text: Hd-rR

characteristic: strain

text: BioSample entry for Oryzias latipes strain Hd-rR, complete genome assembly


Solanum lycopersicum genome

characteristic: ENA-CHECKLIST

text: EBI

text: public

text: Solanum lycopersicum genome

text: ENA

text: tomato

characteristic: cultivar

text: Solanum lycopersicum

text: Solanum lycopersicum

text: BioSample entry for Solanum lycopersicum genome


E-MTAB-3375:Sample 15

text: public

text: E-MTAB-3375:Sample 15

tag: Namespace:The Garvan Institute of Medical Research

text: ArrayExpress

characteristic: broker name

text: Norway rat

text: Protocols: Animals were decapitated at indicated time points after HFS induction and the dentate gyrus (DG) and the cornu amonis (CA) of the hippocampus were rapidly micro dissected on ice. After dissection, the tissue was flash frozen on dry ice and stored at -80 until use. The tissues were homogenized (6,500 rpm, 2 cycles á 15 sec) in 600 μl cold lysis buffer (AllPrep DNA/RNA Mini Kit, Qiagen) or in 500 ul Trizol, using the Prelyse system (Prelyse™ 24, Bertin Technologies, Montigny-le-Bretonneux, France) and recommended ceramic beads (03961ck14). The electrophysiology procedures have been detailed elsewhere (Messaoudi et al., 2002; Panja et al., 2009). Briefly, adult male Sprague-Dawley rats were anesthetized with urethane and electrodes were inserted for selective stimulation the medial perforant pathway and recording of evoked potentials in the hilus region of the dentate gyrus. Recordings were done with borosilicate glass micropipettes (tip size 4-8 µm) filled with 1M NaCl (input impedance 3-4 MΩ). Test pulses were applied at 0.033 Hz thought the experiment except during the period of HFS. The HFS paradigm for LTP induction consisted of eight pulses at 400 Hz, repeated four times, at 10 s intervals. Three sessions of HFS were given, with 5 minutes between each HFS. The baseline stimulated group received test pulses (0.033 Hz) only without the HFS. CPP ((R,S)-3-22-carboxypiperazin-4-yl-propyl-1-phosphonic acid; Tocris, #0173) was dissolved in saline and injected IP at a dose of 10 mg/kg, 90 min prior to HFS. Signals from the dentate hilus were amplified, filtered (1 Hz to 10 kHz), and digitized (25 kHz). Acquisition and analysis of field potentials were accomplished using Data Wave Technologies Work Bench Software (Longmont, CO). The maximum slope of the fEPSP and the amplitude of the population spike measured from its negative going apex to the tangent line, joining the first two positive peaks were measured and the averages of four consecutive responses were obtained. The RNA for RNA-seq experiment II was isolated using Allprep RNA/DNA isolation kit (Qiagen) with on-column DNase treatment. RNA from primary hippocampal cell cultures was isolated with Trizol, dissolved in nuclease free water and treated with Turbo DNase to remove genomic DNA (Ambion).Trizol purified RNA was finally precipitated and dissolved in 1mM EDTA. RNA samples were evaluated by ultraviolet spectroscopy for purity and concentration (NanoDrop 2000 Spectrophotometer, Thermo Scientific) and were assessed further for RNA integrity on the Agilent 2100 Bioanalyzer (Agilent Technologies). All analyzed samples had an RNA Integrity Number (RIN) of 8 or better. Total RNA was stabilized with RNAstable® (Biomatrica, San Diego, CA, USA) and sent to the Garvan Institute of Medical Research , Sydney, Australia) for RNA library construction and subsequent RNA sequencing. RNA stabilized with RNAstable® was resuspended with nuclease-free H2O. The 4 experimental treatment groups were 30 min LTP, 2h LTP, 5h LTP and naïve tissue (3 biological replicates for experimental and control dentate gyrus = 24). RNA quality was assessed with an Agilent Technologies 2100 Bioanalyzer with an RNA 6000 Nano kit (Agilent Technologies, USA) according to the manufacturer’s instructions. RNA concentration was measured using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). 500 ng of total RNA was used as input material for library preparation using the TruSeq Stranded Total RNA Sample Prep Kit (LT) (Illumina, USA) according to manufacturer’s instructions. Individual libraries were indexed as recommended by Illumina. Indexed cDNA libraries were analysed individually using an Agilent Technologies 2100 Bioanalyzer with the DNA 1000 kit according to the manufacturer’s instructions (Agilent Technologies, USA). Libraries were diluted and pooled to a final concentration of 10 nM each in nuclease-free H2O (Ambion, USA). Pooled libraries were quantitated using a Life Technologies Qubit 2.0 Fluorometer with the Qubit dsDNA HS Assay Kit (Life Technologies, USA) and further diluted to 2 nM. Final DNA library concentration was confirmed using a Qubit dsDNA HS Assay Kit. PCR-competent library DNA concentration was verified using the universal KAPA Library Quantification Kit for Illumina Sequencing Platforms according to manufacturer’s instructions (KAPA Biosystems, USA). An Applied Biosystems 7900HT Fast Real-Time PCR machine (Life Technologies, USA) was used for quantitative real-time

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Stimulated

characteristic: stimulus

text: 2 hour

characteristic: timepoint

text: Sample 15

characteristic: title


E-MTAB-3375:Sample 18

text: The Garvan Institute of Medical Research

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3375:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: Norway rat

characteristic: common name

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 2 hour

text: Sample 18


E-MTAB-3375:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-3375:Sample 16

text: ArrayExpress

text: Norway rat

characteristic: description

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 2 hour

text: Sample 16


E-MTAB-3375:Sample 6

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-3375:Sample 6

text: ArrayExpress

characteristic: broker name

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: Control hour

text: Sample 6


E-MTAB-3375:Sample 3

text: public

text: E-MTAB-3375:Sample 3

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Unstimulated

characteristic: stimulus

text: Control hour

text: Sample 3


E-MTAB-3375:Sample 2

characteristic: INSDC first public

text: public

text: E-MTAB-3375:Sample 2

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: Control hour

characteristic: timepoint

text: Sample 2

characteristic: title


E-MTAB-3375:Sample 24

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3375:Sample 24

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 5 hour

text: Sample 24


E-MTAB-3375:Sample 21

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-3375:Sample 21

characteristic: Submitter Id

text: ArrayExpress

text: Norway rat

characteristic: common name

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimulated

text: 5 hour

text: Sample 21


E-MTAB-3375:Sample 19

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-3375:Sample 19

text: ArrayExpress

text: Norway rat

characteristic: description

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimulated

text: 5 hour

text: Sample 19


E-MTAB-3375:Sample 1

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-3375:Sample 1

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: Control hour

text: Sample 1


E-MTAB-3375:Sample 10

text: public

text: E-MTAB-3375:Sample 10

text: ArrayExpress

characteristic: broker name

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Unstimulated

text: 0.5 hour

text: Sample 10


E-MTAB-3375:Sample 4

characteristic: INSDC first public

text: public

text: E-MTAB-3375:Sample 4

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

characteristic: stimulus

text: Control hour

characteristic: timepoint

text: Sample 4


E-MTAB-3375:Sample 13

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3375:Sample 13

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimulated

text: 2 hour

text: Sample 13

characteristic: title


E-MTAB-3375:Sample 22

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-3375:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 5 hour

text: Sample 22


E-MTAB-3375:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-3375:Sample 5

text: ArrayExpress

text: Norway rat

characteristic: common name

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: Control hour

text: Sample 5


E-MTAB-3375:Sample 12

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-3375:Sample 12

text: ArrayExpress

text: Norway rat

characteristic: description

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 0.5 hour

text: Sample 12


E-MTAB-3375:Sample 23

text: public

text: E-MTAB-3375:Sample 23

text: ArrayExpress

characteristic: broker name

text: Norway rat

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: left side dentate gyrus

characteristic: organism part

text: Rattus norvegicus

text: male

text: Stimulated

text: 5 hour

text: Sample 23


E-MTAB-3375:Sample 7

characteristic: INSDC first public

text: public

text: E-MTAB-3375:Sample 7

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

characteristic: sex

text: Stimulated

characteristic: stimulus

text: 0.5 hour

text: Sample 7


E-MTAB-3375:Sample 17

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3375:Sample 17

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimualted

text: 2 hour

characteristic: timepoint

text: Sample 17


E-MTAB-3375:Sample 11

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: E-MTAB-3375:Sample 11

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimulated

text: 0.5 hour

text: Sample 11

characteristic: title


E-MTAB-3375:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-3375:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left side dentate gyrus

text: Rattus norvegicus

text: male

text: Stimulated

text: 0.5 hour

text: Sample 9


E-MTAB-3375:Sample 8

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-3375:Sample 8

text: ArrayExpress

text: Norway rat

characteristic: common name

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Umstimulated

text: 0.5 hour

text: Sample 8


E-MTAB-3375:Sample 20

text: public

text: E-MTAB-3375:Sample 20

text: ArrayExpress

characteristic: broker name

text: Norway rat

characteristic: description

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: right side dentate gyrus

text: Rattus norvegicus

text: male

text: Unstimulated

text: 5 hour

text: Sample 20


E-MTAB-3375:Sample 14

characteristic: INSDC first public

text: public

text: E-MTAB-3375:Sample 14

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: right side dentate gyrus

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Unstimulated

text: 2 hour

text: Sample 14


text: 2-3 months

characteristic: ArrayExpress-Age

text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 wildtype GFP

characteristic: ArrayExpress-Genotype

text: GFP IP

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: ChIP DNA from sorted neurons from MeCP2 GFP mouse brain

characteristic: sample description

text: Mus musculus

characteristic: strain


text: 2-3 months

text: Glia

text: Adult

text: MeCP2 wildtype GFP

text: GFP IP

text: Brain

text: male

text: mouse

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: ChIP DNA from sorted glia from MeCP2 GFP mouse brain

text: Mus musculus


text: 2-3 months

characteristic: ArrayExpress-Age

text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 wildtype GFP

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: male

text: mouse

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Input DNA from sorted neurons from MeCP2 GFP mouse brain

text: Mus musculus


text: 2-3 months

text: Glia

text: Adult

text: MeCP2 wildtype GFP

text: Brain

text: male

characteristic: ArrayExpress-Sex

text: mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: Input DNA from sorted glia from MeCP2 GFP mouse brain

characteristic: sample description

text: Mus musculus


text: 2-3 months

text: Brain Cells

text: Adult

text: symptom free

characteristic: ArrayExpress-DiseaseState

text: MeCP2 R133C GFP

text: Brain

text: male

text: mouse

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Input DNA from total brain from R133C GFP mutant mouse

text: Mus musculus

characteristic: strain


text: 2-3 months

characteristic: ArrayExpress-Age

text: Brain Cells

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: symptom free

text: MeCP2 R133C GFP

text: GFP IP

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

text: male

text: mouse

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: ChIP DNA from total brain from R133C GFP mutant mouse

text: Mus musculus


text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EMBL Heidelberg

text: public

characteristic: biological replicate

text: ArrayExpress

characteristic: broker name

text: embryonic stem cells

characteristic: cell type

text: house mouse

text: Protocols: Mouse embryonic stem cells (ESCs), were derived from eight to ten-weeks-old c-myc(fl/fl); N-myc(fl/fl); RosaEYFP(fl/fl) 12,21,22 or c-Myc(eGFP/eGFP) 19 mice. ESCs were cultured in 2i on Gelatine-coated dishes. Deletion of the flox alleles in c-myc(fl/fl) N-myc(fl/fl) RosaEYFP(fl/fl) ESCs was induced by transient transfection of EF1a-mCherry-CRE plasmid using Lipofectamine 2000 (Invitrogen, 1639722) according to the manufacturer’s instructions. mCherry-positive and negative cells were sorted by flow cytometry 24h after transfection. Total RNA isolation was performed using ARCTURUS(R) PicoPure(R) RNA Isolation Kit (Life Technologies, Invitrogen) according to the manufacturer’s instructions. Total RNA was used for quality controls and for normalization of starting material. cDNA-libraries were generated with 10 ng of total RNA using the SMARTer(TM) Ultra Low RNA Kit for Illumina Sequencing (Clontech) according to the manufacturer’s indications.

characteristic: description

text: c-myc(mut/mut) N-myc(mut/fl)

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: c-myc homozygous deletion, N-myc hetrozygous deletion

characteristic: phenotype

text: Mus musculus

characteristic: technical replicate

text: 24 hour

characteristic: time

characteristic: title

text: mcherry-cre

characteristic: transfection


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL Heidelberg

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cells

text: house mouse

characteristic: common name

text: c-myc(fl/fl) N-myc(fl/fl)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type

text: Mus musculus

text: control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EMBL Heidelberg

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: biological replicate

text: ArrayExpress

text: embryonic stem cells

text: house mouse

text: c-myc(mut/mut) N-myc(mut/fl)

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 96 hour

text: mcherry-cre


text: EMBL Heidelberg

text: public

text: ArrayExpress

characteristic: broker name

text: embryonic stem cells

characteristic: cell type

text: house mouse

characteristic: description

text: c-myc(fl/fl) N-myc(fl/fl)

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: c-myc, N-myc double knock-out

characteristic: phenotype

text: Mus musculus

characteristic: technical replicate

text: 96 hour

text: mcherry-cre


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL Heidelberg

characteristic: INSDC center name

text: public

text: ArrayExpress

text: embryonic stem cells

text: house mouse

text: c-myc(fl/fl) N-myc(fl/fl)

text: Mus musculus

text: Mus musculus

text: c-myc, N-myc double knock-out

text: Mus musculus

text: 24 hour

characteristic: time

characteristic: title

text: mcherry-cre

characteristic: transfection


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EMBL Heidelberg

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cells

text: house mouse

characteristic: common name

text: c-myc(mut/mut) N-myc(mut/fl)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type

text: Mus musculus

text: control


text: European Molecular Biology Laboratory

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:European Molecular Biology Laboratory

text: ArrayExpress

characteristic: broker name

text: white-tufted-ear marmoset

text: Protocols: Embryos were collected by non-surgical uterine flush between E5.0 and E7.0. Marmoset blastocysts were staged based on embryonic day, diameter and blastocoel formation. Where appropriate, zona pellucidae were removed using acid Tyrode's solution (Sigma) and embryos were subjected to immunosurgery as previously described (Solter and Knowles, Proc Natl Acad Sci USA 72:5099-102; Nichols et al. Cell 95:379-91). Briefly, blastocysts were incubated for 15min with a custom rabbit polyclonal anti-marmoset antibody in N2B27 medium, washed in N2B27 and further incubated for 15min in a 1:5 dilution of rat serum (in-house) in N2B27 for the complement reaction. Embryos were transferred into N2B27 without washing and incubated for 15min to allow efficient cell lysis. The ICM was subsequently dissociated from residual trophectoderm with a narrowly fitting glass pipette. For postimplantation embryos the epiblast was isolated by manual dissection. Extraembryonic ectoderm was first removed with a wide mouth-controlled pipette, and the visceral endoderm was then mechanically separated by drawing the embryonic portion through a narrow glass aperture. Dissociation of morulae, ICMs and postimplantation epiblast was performed in a 1:1 mixture of 0.025% trypsin plus EDTA (Invitrogen) and 0.025% trypsin (Invitrogen) plus 1% chick serum (Sigma). Morulae and early ICMs were incubated for 5min at 37 C, and E4.5 ICMs and postimplantation epiblasts were incubated for 10min. The explants were washed in N2B27 supplemented with 10µM HEPES and 1.5mg/ml BSA (Gibco) and dissociated in a small drop of medium using blunted microcapillaries with an inner diameter large enough to accommodate approximately 2-3 cells. Cells were transferred immediately into 4.5ul of single-cell lysis buffer and snap frozen on dry ice. cDNA synthesis was performed using whole transcriptome preamplification (Tang et al. Nat Methods 6:377-382, Tang et al. Nat Protoc. 5:516-535). cDNA was preamplified for 20 cycles using a single-temperature protocol in a Veriti 96 Well Thermal Cycler (Applied Biosystems). Preamplified cDNA samples were sheared by ultrasonication on a Covaris S2 for 80s with the following parameter settings: Duty Cycle = 10, Cycles per Burst = 200, Intensity = 4. Samples were ethanol precipitated and resuspended in Tris buffer for subsequent enzymatic modifications. End repair was carried out with T4 DNA polymerase and T4 polynucleotide kinase (New England Biolabs) and samples were purified with Ampure XP paramagnetic beads (Beckman Coulter). Blunt-end, 3'-phosphorylated products were 3'-adenylated with exo- Klenow fragment in the presence of dATPs (New England Biolabs), purified with Ampure beads and ligated to sequencing adapters (Illumina) by T4 DNA ligase at 20 C for 30 minutes. PCR amplification of library constructs was carried out with AccuPrime DNA polymerase (Invitrogen) for 13 cycles on a Bio-Rad C1000 thermocycler using denaturing and annealing conditions optimized for even A/T vs. G/C processing (Aird et al. Genome Biol 12:R18). Molarity and size distribution of sequencing libraries were assessed by DNA 1000 microfluidic chips on the 2100 Bioanalyzer (Agilent).

text: late-stage blastocyst

characteristic: developmental stage

text: Callithrix jacchus

text: Callithrix jacchus

text: embryo

characteristic: organism part

text: Callithrix jacchus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: European Molecular Biology Laboratory

text: public

text: ArrayExpress

text: white-tufted-ear marmoset

characteristic: description

text: early blastocyst

text: Callithrix jacchus

text: Callithrix jacchus

text: embryo

text: Callithrix jacchus


text: European Molecular Biology Laboratory

characteristic: INSDC center name

text: public

text: ArrayExpress

text: white-tufted-ear marmoset

text: mid-stage blastocyst

text: Callithrix jacchus

text: Callithrix jacchus

text: embryo

text: Callithrix jacchus

characteristic: title


characteristic: ENA-CHECKLIST

text: NCGR

text: public

text: Japanese rice

text: ssRNA-seq of polyA selected sample from rice leaf

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group


characteristic: ENA-FIRST-PUBLIC

text: UPPSALA UNIVERSITET

text: public

tag: Namespace:UPPSALA UNIVERSITET

text: Bjorn Brunstrom

characteristic: collected by

text: chicken

text: Female4Brain White Leghorn chicken embryo

text: 18 day embryo

characteristic: dev stage

text: Sweden

characteristic: geographic location (country and/or sea)

text: Gallus gallus

text: Gallus gallus

text: female

characteristic: sex

text: White Leghorn

characteristic: strain

text: brain

characteristic: tissue type

text: White Leghorn

characteristic: variety


text: UPPSALA UNIVERSITET

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bjorn Brunstrom

text: chicken

characteristic: common name

text: Female4Bursa White Leghorn chicken embryo

text: 18 day embryo

text: Sweden

text: Gallus gallus

characteristic: organism

text: Gallus gallus

text: female

text: White Leghorn

text: bursa

text: White Leghorn


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITET

characteristic: INSDC center name

text: public

text: Bjorn Brunstrom

text: chicken

text: Female4Heart White Leghorn chicken embryo

characteristic: description

text: 18 day embryo

text: Sweden

text: Gallus gallus

text: Gallus gallus

text: female

text: White Leghorn

text: heart

characteristic: title

text: White Leghorn


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UPPSALA UNIVERSITET

text: public

text: Bjorn Brunstrom

characteristic: collected by

text: chicken

text: Female4Kidney White Leghorn chicken embryo

text: 18 day embryo

characteristic: dev stage

text: Sweden

characteristic: geographic location (country and/or sea)

text: Gallus gallus

text: Gallus gallus

text: female

text: White Leghorn

text: kidney

text: White Leghorn


text: UPPSALA UNIVERSITET

characteristic: INSDC first public

text: public

text: Bjorn Brunstrom

text: chicken

text: Female4Liver White Leghorn chicken embryo

text: 18 day embryo

text: Sweden

text: Gallus gallus

text: Gallus gallus

text: female

characteristic: sex

text: White Leghorn

characteristic: strain

text: liver

characteristic: tissue type

text: White Leghorn

characteristic: variety


text: UPPSALA UNIVERSITET

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bjorn Brunstrom

text: chicken

characteristic: common name

text: Female4Lung White Leghorn chicken embryo

text: 18 day embryo

text: Sweden

text: Gallus gallus

text: Gallus gallus

text: female

text: White Leghorn

text: lung

text: White Leghorn


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITET

characteristic: INSDC center name

text: public

text: Bjorn Brunstrom

text: chicken

text: Female4Muscle White Leghorn chicken embryo

characteristic: description

text: 18 day embryo

text: Sweden

text: Gallus gallus

characteristic: organism

text: Gallus gallus

text: female

text: White Leghorn

text: muscle

text: White Leghorn


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UPPSALA UNIVERSITET

text: public

text: Bjorn Brunstrom

characteristic: collected by

text: chicken

text: Female4Ovarium White Leghorn chicken embryo

text: 18 day embryo

characteristic: dev stage

text: Sweden

text: Gallus gallus

text: Gallus gallus

text: female

text: White Leghorn

text: ovary

characteristic: title

text: White Leghorn


text: UPPSALA UNIVERSITET

characteristic: INSDC first public

text: public

text: Bjorn Brunstrom

text: chicken

text: Female4Spleen White Leghorn chicken embryo

text: 18 day embryo

text: Sweden

characteristic: geographic location (country and/or sea)

text: Gallus gallus

text: Gallus gallus

text: female

characteristic: sex

text: White Leghorn

characteristic: strain

text: spleen

characteristic: tissue type

text: White Leghorn