characteristic: External Id

text: public

text: ArrayExpress

text: fission yeast

characteristic: common name

text: Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). Cells were harvested and fixed for 20 minutes with 1% formaldehyde (Larochelle et al. NAR 2012, 40:10240) Whole cell extract from fixed cells was prepared as described in (Larochelle et al. NAR 2012, 40:10240) ChIP experiments were performed as described in (Larochelle et al. NAR 2012, 40:10240). For ChIP-seq experiments, chromatin immunoprecipitation was done as described above with the following modifications. We scaled up the reactions to obtain larger amount of chromatin for library preparation. Chromatin was immunoprecipitated with Pan mouse IgG Dynabeads (Invitrogen) antibody against Rpb1 (8WG16, Convance). The 8WG16 antibody is known to recognize the C-terminal domain (CTD) of Rpb1 and measures total RNAPII along genes in yeast. Libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina kit (New England BioLabs) according to the manufacturer instructions.

text: h+ his7-366 leu1-32 ura4-d18 ade6-m210 TFS1::kanMX6 p81NMT1-DIS3::natMX6

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fission yeast

text: Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). Cells were harvested and fixed for 20 minutes with 1% formaldehyde (Larochelle et al. NAR 2012, 40:10240) Whole cell extract from fixed cells was prepared as described in (Larochelle et al. NAR 2012, 40:10240) ChIP experiments were performed as described in (Larochelle et al. NAR 2012, 40:10240). For ChIP-seq experiments, chromatin immunoprecipitation was done as described above with the following modifications. We scaled up the reactions to obtain larger amount of chromatin for library preparation. Chromatin was immunoprecipitated with Pan mouse IgG Dynabeads (Invitrogen) coated with antibody against the HA tag (12CA5, Roche). Libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina kit (New England BioLabs) according to the manufacturer instructions.

characteristic: description

text: h- rpb3-3HA::KanMX6 p81nmt1::natMX6-dis3

characteristic: genotype

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

characteristic: sex

characteristic: strain

characteristic: title

type: has member


characteristic: External Id

text: MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK

text: public

text: ArrayExpress

text: fission yeast

characteristic: common name

text: h+ ade6-M216 leu1-32 ura4-D18 his3-D1

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: fission yeast

text: Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). Total RNA was isolated from S. pombe cells using a hot phenol method followed by phenol-chloroform extractions, and precipitation as described (Lyne et al., 2003, BMC Genomics 4, 27.). 10ug total RNA was used as starting material for strand-specific RNA-seq library preparation using an unreleased early version provided by Illumina of the Illumina TruSeq Small RNA Sample Prep Kit. Briefly: poly(A)+ RNA was enriched by two rounds of poly(dT) Sera-Mag magnetic beads purification. Enriched poly(A)+ RNA was then fragmented to an average size of ~200nt. Fragmented RNA was 3' de-phosphorylated with Antartic phosphatase and 5' phosphorylated with polynucleotide kinase this treatment prepares RNA fragments for subsequent ligation of Illumina RNA adaptors to their 5' and 3' ends using a 3' RNA ligase and a T4 RNA ligase, respectively. First strand cDNA was produced using a primer specific for the Illumina 3' adaptor. The library was finally amplified with 15 cycles of PCR using primers specific for the Illumina adaptors and purified using SPRI-beads (Agencourt, Beckman Coulter). Libraries size distributions and concentrations were determined on an Agilent Bioanalyzer

text: h? ade6? leu1-32 ura4-D18 his3-D1 p81nmt1::kanMX6-mtr4

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: ArrayExpress

text: fission yeast

text: h+ his7-366 leu1-32 ura4-d18 ade6-m210 p81NMT1-DIS3::natMX6

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fission yeast

text: h? ade6? leu1-32 ura4-D18 his3-D1 p81nmt1::kanMX6-dis3

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: fission yeast

text: h+ his7-366 leu1-32 ura4-d18 ade6-m210 TFS1::kanMX6

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fission yeast

text: h? ade6-M21? leu1-32 ura4-D18 his3-D1 p81nmt1::kanMX6-rrp41

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

text: ArrayExpress

text: fission yeast

characteristic: description

text: h+ his7-366 leu1-32 ura4-d18 Ade6_m210

characteristic: genotype

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

text: ArrayExpress

text: fission yeast

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

text: ArrayExpress

text: fission yeast

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

characteristic: strain


text: public

text: ArrayExpress

text: fission yeast

text: h+ ade6-M216 leu1-32 ura4-D18 his3-D1

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

characteristic: sex

characteristic: title


characteristic: External Id

text: public

tag: Namespace:Centre for Organismal Studies (COS) - University of Heidelberg

text: ArrayExpress

characteristic: cell line

text: neural stem cells

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Neural stem cells cultured in proliferation-promoting medium Total RNA extracted using Trizol reagent (Life Technologies) and purified on RNEasy columns (Qiagen) for three independent samples Sequencing libraries prepared from 2 ug of total RNA according to the TruSeq RNA sample preparation v2 protocol (Illumina)

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Centre for Organismal Studies (COS) - University of Heidelberg

characteristic: INSDC center name

text: public

text: ArrayExpress

text: neural stem cells

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus


text: University of Manchester

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Manchester

text: ArrayExpress

text: embryonic stem cell from blastocyst

text: house mouse

text: Protocols: ES cells were maintained in Ndiff N2B27 media in the presence (ES conditions) of CHIR99021 (3uM) and PD032590 (1uM). Control RNAi, Oct4 RNAi or Otx2 RNAi, in the presence (ES conditions) or absence (Differentiation, 12hr) of CHIR99021 (3uM) and PD032590 (1uM). RNA was isolated using RNeasy plus mini Kit (QIAGEN) followed by DNaseI treatment (Ambion, Turbo DNA-free) and re-purify by Agencourt RNA clean XP beads (Beckman coulter) then stored at -80C SENSE mRNA-seq library Prep kit (Lexogen)

text: embryo

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state


text: public

text: low faecal Salmonella shedding

characteristic: Salmonella shedding status

tag: Namespace:University of Alberta and Livestock Gentec

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pig

text: Protocols: Samples used in this study were selected from Salmonella negative piglets (crossbred or Yorkshire sows bred to boars from different breeds) belonging to two populations of 40 and 77 individuals each and raised in climate-controlled, fully enclosed isolation facilities at the USDA-ARS-National Animal Disease Center (NADC) in Ames, IA under identical management conditions. At 7 weeks of age, these Salmonella negative pigs received intranasal inoculation of 109 colony-forming units (cfu) of nalidixic acid resistant Salmonella enterica serovar Typhimurium, ST _4232. Approximately 2.3 ml of whole blood was collected from the jugular vein into PAXgene Blood RNA tubes (processed according to manufacturer's instructions) from each individual just prior to infection (day 0) and at days 2, 7, 14, and 20 post-inoculation. Blood samples collected at day 0 and day 2 from 16 selected pigs identified as low shedding (n=8) and persistent shedding (n=8) based on extremes of Salmonella shedding levels post innoculation were used for total RNA extraction Total RNA was prepared from 4.5-9.0 ml of solution from the PAXgene Blood RNA tubes. The DNA was removed by in-solution DNase I digestion and RNeasy mini elute kit cleanup as recommended by QIAGEN. PCR assay without reverse transcription was used to confirm that the RNA samples were DNA-free. The quantity and quality of the RNA were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and Nanodrop 2000 (Thermo Scientific, Wilmington, DE). A globin reduction protocol developed in-house (Choi et al.; manuscript under preperation) using porcine specific oligonucleotides was performed. Briefly, 3 ug of denatured total RNA was hybridized in a thermal cycler at 70 degrees C for 2 min with the 10X GR oligonucleotides mix in hybridization buffer (100 mM Tris-HCL, pH 7.6; 200 mM KCl) at 70 degrees C for 5 min, then cooled to 4 degrees C. RNA-DNA hybrids were digested with 2 U RNase H (Ambion) in the reaction buffer (100 mM Tris_HCl, pH 7.6, 20 mM MgCl2, 0.1 mM DTT, Superase-in) at 37 degrees C for 10 min and cooled to 4 degrees C. The reaction was stopped by addition of 0.5 M EDTA. The globin depleted RNA was immediately purified with the RNeasy MinElute cleanup kit (Qiagen, Toronto, Canada, Cat. No.: 74204) according to manufacturer's instruction. RNA quality of the globin depleted samples was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, USA). The mRNA library was constructed using Illumina System (TruSeq RNA sample Preparation v2 Guide, Illumina, Inc., San Diego, USA). One ug of total RNA was purified using poly-T oligo-attached magnetic beads. The Poly (A) RNA was primed and fragmented. The first and second strand cDNA were synthesized according to Illumina's sample preparation guide. Then individual RNA index was ligated to the end-repaired cDNA and subsequently enriched. The quality of the mRNA libraries were confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies, Inc.). The quantification was performed using StepOne Real-Time PCR System (Applied Biosystems, Carlsbad, USA). The individual libraries were adjusted to 2 nM concentrations and pooled before denaturation and dilution according to Illumina's instructions. The diluted libraries (8 - 10 pM) were loaded on a cBot (Illumina) for cluster generation using the TruSeq SR Cluster Kit v3 (50 cycles, Illumina).

characteristic: description

text: none (mock)

characteristic: infect

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: whole blood

characteristic: organism part

text: Sus scrofa

text: frozen specimen

text: 0 day

characteristic: time post infection


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: University of Alberta and Livestock Gentec

text: public

text: low faecal Salmonella shedding

text: ArrayExpress

text: pig

characteristic: common name

text: Salmonella enterica serovar Typhimurium

text: Sus scrofa

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen

text: 2 day

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: persistent faecal Salmonella shedding

text: ArrayExpress

text: pig

text: Salmonella enterica serovar Typhimurium

text: Sus scrofa

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen

text: 2 day


text: public

characteristic: SRA accession

text: low faecal Salmonella shedding

characteristic: Salmonella shedding status

characteristic: Submitter Id

text: ArrayExpress

text: pig

text: none (mock)

text: Sus scrofa

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen

characteristic: specimen with known storage state

text: 0 day


text: public

text: persistent faecal Salmonella shedding

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: none (mock)

characteristic: infect

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: whole blood

text: Sus scrofa

text: frozen specimen

text: 0 day


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#10

text: human

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#11

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#12

text: human

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: MMRI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#13

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#14

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

characteristic: SRA accession

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#15

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#16

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#17

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#18

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#19

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#1

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#20

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#21

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#22

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#23

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#24

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#2

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#3

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#4

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#5

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-42.Neuron-#6

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#10

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#11

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#12

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#13

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#15

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#16

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#17

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#18

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#19

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#1

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#20

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#21

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#22

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#23

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#24

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#2

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#3

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#4

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#5

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#6

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#7

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#8

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-45.Neuron-#9

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#10

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#11

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#12

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#13

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#14

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#15

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#16

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#17

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#18

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#19

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#1

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#20

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#21

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#22

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#23

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#24

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#2

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#3

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#4

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#5

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#6

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#7

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#8

text: human

text: Homo sapiens

text: Homo sapiens


text: MMRI

text: public

text: Hippocampus.Single.WGS.CTRL-55.Neuron-#9

text: human

text: Homo sapiens

text: Homo sapiens


text: male

characteristic: ArrayExpress-Sex

text: Mouse

characteristic: ArrayExpress-Species

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mouse

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: HEIDELBERG UNIVERSITY

text: public

tag: Namespace:HEIDELBERG UNIVERSITY

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: neural stem cells

characteristic: cell type

text: house mouse

text: Protocols: Neural stem cells cultured in proliferation-promoting medium Chromatin Immunoprecipitation with antibodies to Olig2 (Millipore, AB9610), Ascl1 (BD Pharmingen, 556604), Tcf3 (Santa Cruz, sc-349), Max (Santa Cruz, sc-197), NFI (Santa Cruz, sc-30918), Sox2 (Santa Cruz sc-17320), Sox9 (Millipore, AB5535) and Sox21 (R&D systems, AF3538) transcription factors. Libraries were prepared from 10ng of ChIP-enriched DNA using Illumina's ChIP-seq DNA sample preparation kit.

characteristic: description

text: wild type

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Max


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: HEIDELBERG UNIVERSITY

text: public

text: ArrayExpress

text: neural stem cells

text: house mouse

text: wild type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: HEIDELBERG UNIVERSITY

characteristic: INSDC center name

text: public

text: ArrayExpress

text: neural stem cells

text: house mouse

characteristic: common name

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: NFI

characteristic: title


text: Yale University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Yale University

text: ArrayExpress

text: fibroblast

text: human

text: Protocols: IMR90 cells were cultured with MEM Earles media supplemented with 10% FBS 37C with 5% CO2 When cells are 80% confluent, Sendai Virus or media was added to the media and incubated for 6 hours at 37C. We have followed the protocol previously published in Core, L., Waterfall, J. & Lis, J. Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845-1848 (2008). Briefly, nuclei from treated cells were isolated and incubated for 5 minutes with nucleotides and bromo-UTP analog. The nascent RNA from the labeling reaction was isolated and subjected to immunoaffinity purification using Br-dUTP antibody (Santa Cruz Biotech). After first round of purification of nascent RNA, the resulting RNA was ligated with 5' small RNA adaptor RNA (Illumina) and subjected to second Br-UTP purififcation. The resulting RNA is futher ligated using 3' adaptor RNA and subjected to third round of purification. The final purified RNA was reverse transcribed and PCR amplified and size selected (150-250bp) using polyacrylamide gel electrophoresis to generate the final sequencing library. A modified small RNA library contruction protocol based on the Illumina kit was modified and incorporated with the EXTRACTION PROTOCOL. Please see the EXTRACTION PROTOCOL for details.

text: Sendai virus

characteristic: infect

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: 6 hour

characteristic: time


text: Yale University

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: fibroblast

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: 0 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

tag: Namespace:CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

text: ArrayExpress

text: M-CSF cultured bone marrow-derived macrophage

text: house mouse

text: Protocols: The primary macrophages were derived from mouse bone marrow derived cells by culturing them in the presence of M-CSF (protocol according to Gilchrist M et al. Nature 2006, PMID: 16688168) and were treated with 6ug/ml polyI:C (Invivogen) Qiazol protocol (according to manual) Truseq kit from Illumina

text: wild type

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: frozen specimen

characteristic: specimen with known storage state

text: 2 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 4

characteristic: ENA-LAST-UPDATE

text: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

characteristic: common name

text: genetrap

characteristic: genetic modification

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour

characteristic: title


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 1

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour

characteristic: time


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: cell type

text: house mouse

characteristic: description

text: genetrap

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 14

characteristic: External Id

text: public

text: ArrayExpress

text: house mouse

text: genetrap

text: Setdb2 gt/gt

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: frozen specimen

characteristic: specimen with known storage state

text: 8 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 11

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 8 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 17

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

characteristic: common name

text: genetrap

characteristic: genetic modification

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 8 hour

characteristic: title


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 3

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour

characteristic: time


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: cell type

text: house mouse

characteristic: description

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 2 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 5

characteristic: External Id

text: public

text: ArrayExpress

text: house mouse

text: genetrap

text: Setdb2 gt/gt

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: frozen specimen

characteristic: specimen with known storage state

text: 2 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 12

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 6

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

characteristic: common name

text: genetrap

characteristic: genetic modification

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 2 hour

characteristic: title


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 13

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: genetrap

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 0 hour

characteristic: time


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: cell type

text: house mouse

characteristic: description

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 8 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 8

characteristic: External Id

text: public

text: ArrayExpress

text: house mouse

text: genetrap

text: Setdb2 gt/gt

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: frozen specimen

characteristic: specimen with known storage state

text: 2 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 9

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: wild type

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 2 hour


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 16

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

characteristic: common name

text: wild type

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 8 hour

characteristic: title


name: E-MTAB-2263:Bone marrow derived macrophages (cell culture) sample 15

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: genetrap

characteristic: genetic modification

text: Setdb2 gt/gt

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: frozen specimen

text: 8 hour


text: Cologne/Bonn, Germany,51° 0'6.00N, 7° 2'18.00E

characteristic: Capturing location

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

characteristic: Mice name

text: house mouse

text: Time course experiment of wild mice moved to the lab

text: Mus musculus

text: Mus musculus

text: Mice fecal samples


characteristic: External Id

text: MPI-EVB

text: public

text: May 2010

characteristic: collection date

text: three-spined stickleback

text: BS19 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

characteristic: tissue type

text: BS19 River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: September 2010

text: three-spined stickleback

characteristic: common name

text: BS46 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS46 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: May 2010

text: three-spined stickleback

text: BS18 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS18 Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

text: September 2010

characteristic: collection date

text: three-spined stickleback

text: BS66 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS66 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS61 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS61 River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS40b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

characteristic: tissue type

text: BS40b Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: May 2010

text: three-spined stickleback

text: BS12 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS12 Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: INSDC status

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS4 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS4 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

characteristic: SRA accession

text: September 2010

text: three-spined stickleback

text: BS52b Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS52b Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: Submitter Id

text: September 2010

characteristic: collection date

text: three-spined stickleback

text: BS54 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS54 Lake Three-spined stickleback


text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

text: BS22 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS22 Lake Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS35 Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

text: BS35 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: September 2010

text: three-spined stickleback

text: BS62 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

characteristic: tissue type

text: BS62 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS7 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS7 River Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS56 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS56 Lake Three-spined stickleback

characteristic: title


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: September 2010

text: three-spined stickleback

text: BS45 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS45 River Three-spined stickleback


text: MPI-EVB

text: public

text: September 2010

characteristic: collection date

text: three-spined stickleback

text: BS63b Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS63b River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS51 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

characteristic: lat lon

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS51 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS10 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

text: BS10 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: September 2010

text: three-spined stickleback

text: BS44 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

characteristic: tissue type

text: BS44 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS5 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS5 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: May 2010

text: three-spined stickleback

text: BS21 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS21 River Three-spined stickleback

characteristic: title


text: MPI-EVB

text: public

characteristic: Submitter Id

text: September 2010

characteristic: collection date

text: three-spined stickleback

text: BS59 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS59 River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS38b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS38b Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: September 2010

text: three-spined stickleback

text: BS43 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS43 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: September 2010

text: three-spined stickleback

text: BS53 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

text: BS53 River Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

text: BS16 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

characteristic: tissue type

text: BS16 Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: June 2010

text: three-spined stickleback

characteristic: common name

text: BS33 Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS33 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: May 2010

characteristic: collection date

text: three-spined stickleback

text: BS24 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS24 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS64b Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS64b Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS3 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS3 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: June 2010

text: three-spined stickleback

text: BS31b Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS31b River Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS48 Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Muscle tissue

text: BS48 Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: September 2010

text: three-spined stickleback

text: BS58b Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

characteristic: tissue type

text: BS58b Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: September 2010

characteristic: collection date

text: three-spined stickleback

characteristic: common name

text: BS49 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS49 River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS41 Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: USA

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS41 River Three-spined stickleback

characteristic: title


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS14 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS14 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: September 2010

text: three-spined stickleback

text: BS57 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS57 River Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS39 Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS39 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: June 2010

text: three-spined stickleback

text: BS36b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

text: BS36b Lake Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: May 2010

characteristic: collection date

text: three-spined stickleback

text: BS8 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

characteristic: tissue type

text: BS8 Lake Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS13 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS13 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS23 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS23 River Three-spined stickleback

characteristic: title


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: May 2010

text: three-spined stickleback

text: BS11 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS11 River Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: June 2010

text: three-spined stickleback

text: BS32b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS32b Lake Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: May 2010

text: three-spined stickleback

text: BS17 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS17 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: September 2010

characteristic: collection date

text: three-spined stickleback

text: BS47 Three-spined stickleback caught during September 2010 in Misty Stream Inlet at Vancouver Island, Canada (50°36'10.40N,-127°15'8.30W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

characteristic: sex

text: Muscle tissue

text: BS47 River Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

characteristic: common name

text: BS50b Three-spined stickleback caught during September 2010 in Misty Lake at Vancouver Island, Canada (50°36'16.40N,-127°15'42.3W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Canada

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

characteristic: tissue type

text: BS50b Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS1 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS1 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: September 2010

text: three-spined stickleback

text: BS60 Three-spined stickleback caught during September 2010 in Skogseidvatn Lake at Fusa, Norway (60°14'41.57N,5°54'55.39E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS60 Lake Three-spined stickleback

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: September 2010

text: three-spined stickleback

text: BS55 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS55 River Three-spined stickleback


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: June 2010

text: three-spined stickleback

text: BS37 Three-spined stickleback caught during June 2010 in Meadow Creek at Alaska, USA (61°34'8.76N,-149°45'36.00W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS37 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: June 2010

characteristic: collection date

text: three-spined stickleback

text: BS42b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS42b Lake Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS20 Three-spined stickleback caught during May 2010 in Westensee at Schleswig-Holstein, Germany (54°17'1.92N,9°56'55.71E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

characteristic: sex

text: Muscle tissue

text: BS20 Lake Three-spined stickleback


text: MPI-EVB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: May 2010

text: three-spined stickleback

text: BS9 Three-spined stickleback caught during May 2010 in Malenter Au at Schleswig-Holstein, Germany (54°12'15.08N,10°33'41.90E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

characteristic: tissue type

text: BS9 River Three-spined stickleback


text: MPI-EVB

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: May 2010

text: three-spined stickleback

text: BS6 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

characteristic: description

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS6 Lake Three-spined stickleback


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

text: BS15 Three-spined stickleback caught during May 2010 in Eider at Schleswig-Holstein, Germany (54°15'49.02N,10°2'24.47E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS15 River Three-spined stickleback

characteristic: title


characteristic: External Id

text: MPI-EVB

text: public

characteristic: SRA accession

text: September 2010

text: three-spined stickleback

text: BS65 Three-spined stickleback caught during September 2010 in Skogseidvatn River at Fusa, Norway (60°15'19.33N,5°55'35.68E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Norway

characteristic: geographic location (country and/or sea)

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS65 River Three-spined stickleback


text: MPI-EVB

text: public

characteristic: Submitter Id

text: June 2010

characteristic: collection date

text: three-spined stickleback

text: BS34b Three-spined stickleback caught during June 2010 in Long Lake at Alaska, USA (61°34'33.96N,-149°46'25.50W). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: USA

characteristic: lat lon

text: Gasterosteus aculeatus

text: Gasterosteus aculeatus

text: Male

text: Muscle tissue

text: BS34b Lake Three-spined stickleback


characteristic: ENA-LAST-UPDATE

text: MPI-EVB

text: public

text: May 2010

text: three-spined stickleback

characteristic: common name

text: BS2 Three-spined stickleback caught during May 2010 in Grosser Ploener See at Schleswig-Holstein, Germany (54°9'21.61N,10°25'48.52E). Muscle tissue was used for DNA extraction using a Qiagen DNA Midi kit following the manufacturer's protocol for high molecular weight DNA.

text: Germany

text: Gasterosteus aculeatus

characteristic: organism

text: Gasterosteus aculeatus

text: Female

text: Muscle tissue

text: BS2 Lake Three-spined stickleback


text: MMRI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC last update

text: public

tag: Namespace:Department of Biochemistry, University of Cambridge, UK

text: ArrayExpress

characteristic: broker name

text: fission yeast

text: Protocols: Cells were grown in yeast extract mediumGood synchrony is essential to achieving high temporal resolution of expression profiles. We therefore used thermosensitive mutants of the meiotic inhibitor Pat1p. Meiosis was induced as follows: cells of the indicated phenotype were grown in minimal medium containing 2% glucose (EMM, Moreno et al., 1991, Methods Enzymol. 194, 795-823) plus 0.5% NH4Cl, and then resuspended in EMM without NH4Cl (EMM-N) and incubated for 14 h at 25C. Meiosis was started by shifting the cells to 34C in the presence of 0.05% NH4Cl. Samples were collected from vegetative cells (time -14 h), and at the indicated times after the temperature shift. at 32C, incubated for 5 minutes with 100 ug/ml cycloheximide, pelleted at 4C and frozen in liquid N2 RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Polyadenylated RNA was purified from 300-450 ug of total RNA using oligo-dT25 magnetic beads (Life Technologies) following the manufacturer's instructions. Purified mRNA was fragmented by mixing 20 ul of mRNA with 20 ul of 2X alkaline fragmentation buffer (2 mM EDTA, 100 mM Na2CO3) followed by incubation for 15 minutes at 95 degrees C. Samples were run on 15% TBE-urea gels (Life Technologies), and fragments of around 28 nucleotides were extracted from the gel. Both mRNA and ribosomal footprint gel-purified samples were processed identically. RNA samples were purified using Purelink RNA microcolumns (Life Technologies) as described by the manufacturer, except that the samples were initially passed through the column in the presence of 70% ethanol (to favour binding of small RNAs). Samples were then treated with poly nucleotide kinase (PNK, Fermentas) and polyadenylated using 12 units of poly-(A) polymerase (NEB) at 37C for 45 minutes. Reverse transcription reactions were performed using custom primers containing an anchored oligo(dT), 4 nucleotides of known sequence used for multiplexing, and 5 random nucleotides that serve as unique molecular identifiers. Reverse transcription products were gel-purified and circularised using CircLigase II (Epicentre), and amplified by PCR with custom library primers for 12 or 15 cycles.

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: pat1 diploid

characteristic: strain


characteristic: ENA-CHECKLIST

text: Department of Biochemistry, University of Cambridge, UK

text: public

characteristic: INSDC status

text: ArrayExpress

text: fission yeast

characteristic: description

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: pat1 diploid


text: Danio rerio

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Instituto Gulbenkian de Ciencia

text: public

characteristic: SRA accession

tag: Namespace:Instituto Gulbenkian de Ciencia

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: neuronal stem cell

characteristic: cell type

text: house mouse

text: Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-IRES-GFP-puro (NS5_control) or pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), the latter sample collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/mL . DNAse-seq samples from NS5 cells were prepared as described by Song et al (Genome Res, 2011). Libraries were generated as in (Boyle et al., 2008; Song and Crawford, 2010) with a slight modification made to the linkers to increase ligation efficiency (Song et al., 2011, Genome Res).

text: pBABE-IRES-GFP-puro

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Instituto Gulbenkian de Ciencia

characteristic: INSDC center name

text: public

text: ArrayExpress

text: neuronal stem cell

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus


text: University of Hohenheim

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University of Hohenheim

text: ArrayExpress

characteristic: broker name

text: white lupine

text: Protocols: Lupinus albus plants were grown in hydroponic culture without phosphorus Total RNA was isolated using RNeasy Plant Mini Kit (Qiagen) Conducted by GATC Biotech AG, Konstanz, Germany

text: pre-emergent

characteristic: developmental stage

text: Lupinus albus

text: Lupinus albus

text: root

characteristic: organism part

text: Lupinus albus

text: frozen specimen

characteristic: specimen with known storage state


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: University of Hohenheim

text: public

characteristic: INSDC status

text: ArrayExpress

text: white lupine

characteristic: description

text: mature

text: Lupinus albus

text: Lupinus albus

text: root

text: Lupinus albus

text: frozen specimen


text: University of Hohenheim

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: white lupine

text: juvenile

text: Lupinus albus

text: Lupinus albus

text: root

text: Lupinus albus

text: frozen specimen


characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-2277:SH-SY5Y_shCtrl_control_replicate1

tag: Namespace:EBML EBI, Wellcome Trust Genome Campus, Hinxton, CB10 1SD

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: HeLa cells were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 micro-g/mL streptomycin at 37 degrees C and 5% CO2.' Lentiviral vectors (pLKO.1) harboring shRNAs targeting human Unkempt were obtained from Sigma (SHCLNG-NM_001080419). To knockdown Unkempt in mouse neurons, we modified the pSilencer1.0 GFP vector (Shen et al., Nature Genetics, 2010) by inserting between the ApaI and EcoRI sites the shRNAs targeting mouse Unkempt mRNA. The targeted sequences (shUnkW: CCCACTCTTCGTGCAGCACAA, shUnk: GGTATCACCTTCGTTACTA) were identified by the Broad Institute RNAi Consortium. SH-SY5Y cells were transfected at 40% confluence using Opti-MEM I Reduced Serum Medium and Lipofectamine 2000 (both Life Technologies) with a pool of siRNA targeting UNK (siGENOME SMART pool, M-022950-01) or a non-targeting siRNA pool (siGENOME Non-Targeting siRNA Pool 1; D-001206-13; both Thermo Scientific Dharmacon) at 25 nM. After 36 hours of transfection, cells were re-transfected with the above siRNA pools and incubated for an additional 24 hours.' Total RNA extracted from triplicates of each control and knockdown cells was DNase-treated and purified using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality was assessed with the 2100 Bioanalyzer (Agilent Technologies).' Sequencing library preparation was carried out using TruSeq RNA Sample Preparation Kit v2 (Illumina).'

text: mock treated with shCtrl

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: EBML EBI, Wellcome Trust Genome Campus, Hinxton, CB10 1SD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2277:SH-SY5Y_shCtrl_control_replicate3

text: ArrayExpress

text: human

characteristic: common name

text: mock treated with shCtrl

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC last update

text: public

text: E-MTAB-2277:SH-SY5Y_shUnk_Unk_knockdown_replicate1

text: ArrayExpress

characteristic: broker name

text: human

text: Unk knockdown with shCtrl

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-2277:SH-SY5Y_shCtrl_control_replicate2

text: ArrayExpress

text: human

text: mock treated with shCtrl

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-2277:SH-SY5Y_shUnk_Unk_knockdown_replicate3

text: ArrayExpress

text: human

text: Unk knockdown with shUnk

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: public

text: E-MTAB-2277:SH-SY5Y_shUnk_Unk_knockdown_replicate2

text: ArrayExpress

text: human

text: Unk knockdown with shUnk

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

characteristic: INSDC status

text: E-MTAB-2278:HeLa_Unk+Gfp_overexpression_replicate_2

text: ArrayExpress

text: HeLa

text: human

text: Protocols: HeLa cells were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 micro-g/mL streptomycin at 37 degrees C and 5% CO2. To create Dox-inducible cell lines, we first transduced HeLa cells with MSCV-rtTA3-IRES-EcoR-PGK-Neo (pRIEN), a retrovirus we constructed in the MSCV backbone (Clontech) to co-express an improved reverse transactivator (rtTA3), the ecotropic receptor, and a neomycin resistance marker. Following G418 selection, stably rtTA3-expressing cells were subsequently infected ecotropically with a second retrovirus expressing a puromycin resistance gene and a TREtight-driven transcript encoding GFP alone (pTt-IGPP) or GFP and either full-length mouse or zebrafish Unkempt protein, or any of the mouse Unkempt-deletion mutants (pTtight-X-IGPP, where X is an ortholog or mutant of Unkempt), which we constructed in a self-inactivating retroviral backbone (pQCXIX, Clontech). To induce transgene expression, double-selected cells were treated with doxycycline (Sigma-Aldrich) at 1 micro-g/ml. To determine the effect of Unkempt on the rate of protein synthesis, we performed ribosome profiling experiment on inducible HeLa cells essentially as described (Ingolia et al., Nature Protocols, 2012), but using the linker and the primers as for the iCLIP experiments (Konig et al., JoVE, 2011). GFP-only or GFP and Unkempt-inducible Hela cells were grown in 15 cm dishes and harvested at 70% confluency. Prior to harvest, cells were treated with Dox for 18 hour to induce the expression of transgenes. Cells were then washed in ice-cold PBS, lysed for 10 minutes on ice in lysis buffer, triturated by passing twice through a syringe fitted with a 26-gauge needle, and spun at 20,000 xg for 10 minutes at 4 degrees C. Cell lysate was digested with RNase I for 45 mintues at room temperature followed by the addition of SUPERase IN to stop nuclease digestion. The lysate was underlayed with 1 M sucrose and spun in a TLA 100.3 rotor at 70,000 rpm for 4 hours at 4 degrees C. Ribosomal pellet was resuspended in Trizol reagent (Life Technologies) and RNA extracted according to the manufacturer's instructions. The extracted RNA was size-selected by denaturing PAGE, retaining only fragments between 26 and 34 nts, and 3' end-dephosphorylated with T4 PNK for 20 minutes at 37 degrees C. Dephosphorylated RNA was precipitated, resuspended, and ligated to a pre-adenylated linker (L3-App) as described for the iCLIP procedure (Konig et al., JoVE, 2011). The ligation mix was precipitated, resuspended, and the ligation products (between 47 and 55 nts) were purified by denaturing PAGE. Reverse transcription (RT) was carried out using barcoded Rclip primers used for the iCLIP experiments (Konig et al., JoVE, 2011) by resuspending the ligation products in 8.5 micro-l water, 0.5 ul Rclip primer (100 micro-M; Rclip primer with a different barcode was used for each sample), and 1 micro-l dNTP (10 mM), with SuperScript III Reverse Transcriptase (Life Technologies) according to the manufacturer's instructions, including the treatment with RNase H. The reaction mix was precipitated and the produced cDNA (between 79 and 87 nts) was purified by denaturing PAGE. RT products were circularized, cut, and precipitated as described for the iCLIP procedure (Konig et al., JoVE, 2011). PCR amplification (12-15 cycles) was carried out using P5/P3 Solexa primers and Accuprime Supermix 1 enzyme (Life Technologies). The prepared cDNA libraries were purified using the Agencourt AMPure XP system (Beckman Coulter).

text: Unk-and-GFP-Tet-on

text: Homo sapiens

text: Homo sapiens

text: Doxycycline-induced GFP and Unk overexpression

characteristic: phenotype

text: Homo sapiens


text: public

characteristic: SRA accession

text: E-MTAB-2278:HeLa_Gfp_expression_control_replicate_2

text: ArrayExpress

text: HeLa

text: human

characteristic: description

text: GFP-Tet-on

text: Homo sapiens

text: Homo sapiens

text: Doxycycline-induced GFP expression control

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-2278:HeLa_Gfp_expression_control_replicate_1

characteristic: Submitter Id

text: ArrayExpress

text: HeLa

text: human

text: GFP-Tet-on

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

text: E-MTAB-2278:HeLa_Unk+Gfp_overexpression_replicate_3

text: ArrayExpress

text: HeLa

characteristic: cell line

text: human

text: Unk-and-GFP-Tet-on

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC center name

text: public

text: E-MTAB-2278:HeLa_Unk+Gfp_overexpression_replicate_1

text: ArrayExpress

text: HeLa

text: human

text: Unk-and-GFP-Tet-on

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: INSDC first public

text: public

text: E-MTAB-2279:SH-SY5Y_Unk_iCLIP_replicate_2

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: HeLa, and SH-SY5Y cells were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 micro-g/mL streptomycin at 37 degrees C and 5% CO2. SH-SY5Y and GFP and Unkempt-inducible HeLa cells were grown in 10 cm plates and harvested at 80% confluency. Prior to harvest, the inducible HeLa cells were treated with Dox for 24 hours to induce expression of Unkempt. iCLIP experiments on SH-SY5Y cells, HeLa cells, and mouse embryonic brain were carried out in replicates as described in Konig et al., JoVE, 2011. Whole brains of E15.5 CD1 mouse embryos were processed as described (Ule et al., Methods, 2005). Briefly, ten whole embryonic brains were isolated and transferred to 6 ml of PBS in a 15 ml tube on ice. Brains were triturated first through a 5 ml serological pipette and then through a 200 ?l pipette tip fitted to a 5 ml serological pipette to partially dissociate the tissue. The suspension was transferred to a 10 cm petri dish and irradiated with UV-light (254 nm) three times at 100 mJ/cm2 on ice, mixing suspension after each irradiation pulse. The irradiated suspension was aliquoted into three 2 ml tubes and centrifuged at 2500 rpm for 3 minutes at 4 degrees C. The supernatant was removed and the cell pellets frozen at -80 degrees C until use. Immunoprecipitation of the cross-linked Unkempt-RNA complexes from all three sample types was carried out using anti-Unkempt antibody (HPA023636, Sigma-Aldrich). Control reactions were performed using normal rabbit IgG antibodies (sc-2027). Library preparation was performed as described in Konig et al, JoVE, 2011.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC last update

text: public

text: ArrayExpress

text: HeLa

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-2279:Mouse_E15_brain_Unk_iCLIP_replicate_2

text: ArrayExpress

characteristic: broker name

text: house mouse

text: 15 day

characteristic: gestational age

text: Mus musculus

text: Mus musculus

text: whole brain

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EBML EBI, Wellcome Trust Genome Campus, Hinxton, CB10 1SD

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-2279:Mouse_E15_brain_Unk_iCLIP_replicate_1

text: ArrayExpress

text: house mouse

text: 15 day

text: Mus musculus

text: Mus musculus

text: whole brain

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-2279:SH-SY5Y_Unk_iCLIP_replicate_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2279:SH-SY5Y_Unk_iCLIP_replicate_4

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: INSDC last update

text: public

text: E-MTAB-2279:Mouse_E15_brain_Unk_iCLIP_replicate_3

text: ArrayExpress

text: house mouse

characteristic: common name

text: 15 day

text: Mus musculus

characteristic: organism

text: Mus musculus

text: whole brain

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-2279:SH-SY5Y_Unk_iCLIP_replicate_3

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

text: E-MTAB-2279:Mouse_E15_brain_Unk_iCLIP_replicate_4

text: ArrayExpress

text: house mouse

text: 15 day

characteristic: gestational age

text: Mus musculus

text: Mus musculus

text: whole brain

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-2279:Mouse_E15_brain_Unk_iCLIP_replicate_5

text: ArrayExpress

text: house mouse

text: 15 day

text: Mus musculus

text: Mus musculus

text: whole brain

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: Universidade Federal do Para

text: public

characteristic: SRA accession

tag: Namespace:Universidade Federal do Para

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: Fresh tissue sample for ultra deep sequencing was obtained from a gastroscopic biopsy (0.4 mm3). The patient was 33 years old, with no evidence of cancer and a normal gastroesophageal junction. Histological examination confirmed normal and healthy conditions. The total small RNA was collected from the tissue samples using the mirVana Isolation Kit (Ambion Inc., US). The concentration and quality were determined using a Nanodrop 1000 spectrophotometer (ND-1000; Nanodrop Technologies, Wilmington, DE), and purification and size selection were performed using 6% polyacrylamide gel electrophoresis Using the SOLiD Small RNA Expression Kit (Ambion Inc., US), 200 ng of small RNA (150-200 bp) was used as a template for the miRNA library. The miRNAs in the library were tagged with unique and specific amplification primers (The barcode system Life Technologies, CA, US). Next, 50 pg of the library was pooled with seven additional miRNA libraries at the same concentration. A fraction of the library pool (0.1 pg) was amplified and fixed on magnetic beads using emulsion PCR. The ePCR product was deposited onto a single slide, and multiplex SOLiD sequencing was used.

text: Homo sapiens

text: Homo sapiens

text: pyloric antrum

text: Homo sapiens


text: male

characteristic: ArrayExpress-Sex

text: Mouse

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mouse

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: TGAC

text: public

text: bread wheat

characteristic: common name

text: Bread wheat line 'STAMM 101'

characteristic: description

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum


characteristic: ENA-CHECKLIST

text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ALBA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ALBATROSS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ALCHEMY'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'AMBROSIA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'APACHE,USA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'AVALON'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'BACANORA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'BATTALION'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'BEAVER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CALIF'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CAPO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CAPELLE-DESPREZ'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CEZANNE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'CORDIALE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'COURTOT'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'DEBEN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'EINSTEIN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'EQUINOX'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ERLA-KOLBEN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'EXSEPT'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'FLAIR'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'GALAHAD'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'GATSBY'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'GLADIATOR'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'GLASGOW'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HEREWARD'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HUMBER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HYBRID-46'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'HYPERION'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'KONTRAST'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MARCO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MEGA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'MERCIA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'NAUTICA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'OAKLEY'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'PARAGON'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'PERLO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RABE,DEU'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RECITAL'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'RIMPAUS-BRAUN'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SAVANNAH'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SCHWEIGERS-TACA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SOLSTICE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SPARK'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SPERBER'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'STARKE2'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'STEADFAST'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'SVALE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'TADORNA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'TARAS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'TREMIE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'TSCHERMAKS'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'VILMORIN-27'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'VIRGO'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'VIRTUE'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'WERLA'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'XI19'

text: Triticum aestivum

text: Triticum aestivum


text: TGAC

text: public

text: bread wheat

text: Bread wheat line 'ZEBEDEE'

text: Triticum aestivum

text: Triticum aestivum