text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: sin_OSN_65-sc-2013-08-12T09:37:57Z-1677615

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


status: PUBLIC

text: OMP-GFP (+/-)

tag: attribute

characteristic: ArrayExpress-Genotype

text: Olfactory epithelium

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: sin_OSN_66-sc-2013-08-12T09:37:59Z-1677616

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: library from single OSNs

characteristic: sample description

text: Mus musculus

characteristic: strain


text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: sin_OSN_67-sc-2013-08-12T09:38:00Z-1677617

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


text: OMP-GFP (+/-)

characteristic: ArrayExpress-Genotype

text: Olfactory epithelium

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

text: SC

text: public

text: sin_OSN_68-sc-2013-08-12T09:38:01Z-1677618

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: sin_OSN_69-sc-2013-08-12T09:38:02Z-1677619

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: sin_OSN_70-sc-2013-08-12T09:38:03Z-1677620

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: sin_OSN_71-sc-2013-08-12T09:38:04Z-1677621

text: house mouse

text: Mus musculus

text: library from single OSNs

text: Mus musculus


text: OMP-GFP (+/-)

text: Olfactory epithelium

text: male

text: Mus musculus

text: SC

text: public

text: sin_OSN_72-sc-2013-08-12T09:38:05Z-1677622

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: library from single OSNs

characteristic: sample description

text: Mus musculus


text: Wildtype

text: Olfactory epithelium

text: male

text: Danio rerio

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: RNA from OE

text: Danio rerio


text: Life Technologies

text: public

tag: Namespace:Life Technologies

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: 20 standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples, and synthetic spikes from homologous microRNA family members. Universal Human miRNA reference RNA (Agilent Technologies, #750700), human brain total RNA (Life Technologies, #AM6050), human liver total RNA (Life Technologies, # AM7960) and MS2 phage RNA (Roche, #10165948001) Ion total RNA-seq v2 kit

characteristic: description

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title

type: has member


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Life Technologies

text: public

text: E-MTAB-1815:serum_+_spiked_miRs_constant_concentration

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: Life Technologies

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-1815:serum_+_spiked_miRs_variable_concentration

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: Life Technologies

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-1815:serum_+_spiked_miRs_constant_concentration_repeat

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: Life Technologies

text: public

characteristic: SRA accession

text: E-MTAB-1815:serum_+_spiked_miRs_variable_concentration_repeat

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: public

tag: Namespace:TRON - Translational Oncology at the Johannes Gutenberg-University Mainz

text: ArrayExpress

text: house mouse

text: Protocols: 5 x 105 BM cells from R26/AE-reconstituted C57BL/6 recipients that had been exposed to DOX for 18 month were injected into secondary C57BL/6 recipients (2 x 5.5 Gy) and the DOX regime was continued for an additional four month For obtaining cells for the following RNA-Seq experiments, 4 x 10^4 L-K+S+IL7Ra-CD34+FcgRII/III+ cells were preparatively sorted using a FACSAria cell sorter (BD). RNA was extracted with the Pico Pure RNA Extraction Kit (Arcturus) according to the manufacturers instructions. The resulting RNA was analysed using an Agilent Bioanalyzer. RNA samples were amplified with Nugen`s Ovation RNA-Seq System and quantified by Qubit measurement. The TruSeq DNA Sample PrepKit (Illumina) was used and the resulting cDNAs were fragmented using AFA technology (Covaris). Subsequently, cDNA fragments were end repaired and 3' adenylated. Illuminas TruSeq adaptors with integrated indices were ligated and the resultant fragments size selected using 2% E-Gels (Invitrogen). Enrichment was done by linear PCR and the resulting fragments were purified using Agencourt AMPure XP magnetic beads.

text: indolent myeloproliferative like myeloid leukaemia

characteristic: disease

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: fresh specimen

characteristic: strain

text: L-GMP


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: TRON - Translational Oncology at the Johannes Gutenberg-University Mainz

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: C57BL/6 recipients (2 x 5.5 Gy) were injected with 5 x 105 non-induced R26/AE BM cells and exposed to DOX for ten days For obtaining cells for the following RNA-Seq experiments, 4 x 10^4 L-K+S+IL7Ra-CD34+FcgRII/III+ cells were preparatively sorted using a FACSAria cell sorter (BD). RNA was extracted with the Pico Pure RNA Extraction Kit (Arcturus) according to the manufacturers instructions. The resulting RNA was analysed using an Agilent Bioanalyzer. RNA samples were amplified with Nugen`s Ovation RNA-Seq System and quantified by Qubit measurement. The TruSeq DNA Sample PrepKit (Illumina) was used and the resulting cDNAs were fragmented using AFA technology (Covaris). Subsequently, cDNA fragments were end repaired and 3' adenylated. Illuminas TruSeq adaptors with integrated indices were ligated and the resultant fragments size selected using 2% E-Gels (Invitrogen). Enrichment was done by linear PCR and the resulting fragments were purified using Agencourt AMPure XP magnetic beads.

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: fresh specimen

text: ST-GMP


text: public

text: ArrayExpress

text: house mouse

text: Protocols: Non-induced compound R26/AE controls For obtaining cells for the following RNA-Seq experiments, 4 x 10^4 L-K+S+IL7Ra-CD34+FcgRII/III+ cells were preparatively sorted using a FACSAria cell sorter (BD). RNA was extracted with the Pico Pure RNA Extraction Kit (Arcturus) according to the manufacturers instructions. The resulting RNA was analysed using an Agilent Bioanalyzer. RNA samples were amplified with Nugen`s Ovation RNA-Seq System and quantified by Qubit measurement. The TruSeq DNA Sample PrepKit (Illumina) was used and the resulting cDNAs were fragmented using AFA technology (Covaris). Subsequently, cDNA fragments were end repaired and 3' adenylated. Illuminas TruSeq adaptors with integrated indices were ligated and the resultant fragments size selected using 2% E-Gels (Invitrogen). Enrichment was done by linear PCR and the resulting fragments were purified using Agencourt AMPure XP magnetic beads.

characteristic: description

text: normal

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

text: Ctrl-GMP

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Department of Immunology, Genetics and Pathology, Genomics, Uppsala University

text: ArrayExpress

characteristic: cell line

text: hepatocellular carcinoma

characteristic: cell type

text: human

text: Protocols: HepG2 cells were cultured to confluence in RPMI 1640 medium supplemented with 10% FBS. Cells were serum-starved over night (1 % FBS) before stimulation with TGF-b1 (final conc. 2.5 ng/ml in complete medium). TGF-b1 treated and control cells were harvested after 1 hour treatment. HepG2 cells were cultured in RPMI 1640 medium with 10% FBS .Cells were serum starved over night with 1% FBS containing medium and treated the cells with 2.5ng/ml (final vomv) of TGFB1.for 1 hour. The cells were washed with PBS and resuspended in ice cold buffer A (0.32M sucrose, 15 mM Hepes pH 7.9, 60Mm KCl , 2 mM EDTA, 0.5 mM EGTA, 0,5 % Bovine serum albumin , 0.5 mM spermidine, 0.15 mM spermine and 0.5 mM DTT). After a short incubation on ice, cells were homogenized with a Dounce homogenizer. The nuclear suspension was slowly layered on an equal volume of ice cold buffer B (30% sucrose, 15 mM Hepes pH 7.9, 60 mM KCl, 2 mM EDTA, 0.5 mM EGTA, 0.5 mM spermidine, 0.15 mM spermine and 0.5 mM DTT) and centrifuged at 3000 rpm for 15 minutes to pellet the nuclei. Collected nuclei were washed once and resuspended in buffer N (0.34 mM sucrose, 15 mM HEPES pH 7.5, 60 mM KCl, 15 mM NaCl, 0.5 mM spermidine, 0.15 mM spermine, and 0.15 mM beta-mercaptoethanol). Samples were adjusted to 3 mM CaCl2 and the nuclear suspension was adjusted to 30 million cells per milliliter and incubated at 37 degrees C for 5 minutes. Micrococcal nuclease (MNase, 300U) was added to each aliquot and incubated for another 5 minutes at 37 degrees C. Buffer S, containing 90 mM HEPES pH 7.9, 220 mM NaCl, 10 mM EDTA, 2% Triton X-100, 0.2% Na-deoxycholate, 0.2%SDS, 0.5 mM PMSF, and 2 ug/ml aprotinin was added to stop the reaction. The solution was centrifuged and the supernatant was used to extract the DNA by Phenol/chloroform/ethanol precipitation. DNA was loaded on 2% agarose gel and the mononucleosome size DNA (147 bp) fragments were excised. Excised gel fragments were purified by using a Quiagen gel extraction kit. Libraries of nucleosomal DNA were prepared according to the SOLiD fragment library protocol and sequenced using v3 chemistry.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

characteristic: sex


text: Department of Immunology, Genetics and Pathology, Genomics, Uppsala University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: hepatocellular carcinoma

text: human

characteristic: common name

text: Protocols: HepG2 cells were cultured to confluence in RPMI 1640 medium supplemented with 10% FBS. HepG2 cells were cultured in RPMI 1640 medium with 10% FBS .Cells were serum starved over night with 1% FBS containing medium and treated the cells with 2.5ng/ml (final vomv) of TGFB1.for 1 hour. The cells were washed with PBS and resuspended in ice cold buffer A (0.32M sucrose, 15 mM Hepes pH 7.9, 60Mm KCl , 2 mM EDTA, 0.5 mM EGTA, 0,5 % Bovine serum albumin , 0.5 mM spermidine, 0.15 mM spermine and 0.5 mM DTT). After a short incubation on ice, cells were homogenized with a Dounce homogenizer. The nuclear suspension was slowly layered on an equal volume of ice cold buffer B (30% sucrose, 15 mM Hepes pH 7.9, 60 mM KCl, 2 mM EDTA, 0.5 mM EGTA, 0.5 mM spermidine, 0.15 mM spermine and 0.5 mM DTT) and centrifuged at 3000 rpm for 15 minutes to pellet the nuclei. Collected nuclei were washed once and resuspended in buffer N (0.34 mM sucrose, 15 mM HEPES pH 7.5, 60 mM KCl, 15 mM NaCl, 0.5 mM spermidine, 0.15 mM spermine, and 0.15 mM beta-mercaptoethanol). Samples were adjusted to 3 mM CaCl2 and the nuclear suspension was adjusted to 30 million cells per milliliter and incubated at 37 degrees C for 5 minutes. Micrococcal nuclease (MNase, 300U) was added to each aliquot and incubated for another 5 minutes at 37 degrees C. Buffer S, containing 90 mM HEPES pH 7.9, 220 mM NaCl, 10 mM EDTA, 2% Triton X-100, 0.2% Na-deoxycholate, 0.2%SDS, 0.5 mM PMSF, and 2 ug/ml aprotinin was added to stop the reaction. The solution was centrifuged and the supernatant was used to extract the DNA by Phenol/chloroform/ethanol precipitation. DNA was loaded on 2% agarose gel and the mononucleosome size DNA (147 bp) fragments were excised. Excised gel fragments were purified by using a Quiagen gel extraction kit. Libraries of nucleosomal DNA were prepared according to the SOLiD fragment library protocol and sequenced using v3 chemistry.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: NCMLS

text: public

text: ATCC

characteristic: biomaterial provider

text: Cell Line

characteristic: biomaterial type

text: human

text: K-562 Cell line

text: erythroleukemia type, CML patient, positive for the bcr:abl fusion gene

characteristic: differentiation method

text: Chronic Myelogenous Leukemia

characteristic: disease

characteristic: line

text: mesoderm

characteristic: lineage

text: RPMI1640 + glutamax 10% FCS and 1% pen/strep

characteristic: medium

text: genomic DNA

characteristic: molecule

text: Homo sapiens

characteristic: organism

text: +/- 11 after obtaining cells from ATCC

characteristic: passage

text: Homo sapiens

text: Female


characteristic: ArrayExpress-CellType

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Th1_rep1-sc-2013-08-14T08:46:30Z-1678025

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: extracted RNA

characteristic: sample description

text: Mus musculus


text: Mus Musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Th1_rep2-sc-2013-08-14T08:46:33Z-1678026

text: house mouse

characteristic: common name

text: Mus musculus

text: extracted RNA

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: Th2_rep1-sc-2013-08-14T08:46:34Z-1678027

text: house mouse

text: Mus musculus

text: extracted RNA

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: Th2_rep2-sc-2013-08-14T08:46:35Z-1678028

text: house mouse

text: Mus musculus

text: extracted RNA

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

characteristic: ArrayExpress-DevelopmentalStage

text: Mus Musculus

text: SC

text: public

text: OS_25_cell1-sc-2013-08-14T09:11:27Z-1678029

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell2-sc-2013-08-14T09:11:30Z-1678030

text: house mouse

text: Mus musculus

characteristic: organism

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell3-sc-2013-08-14T09:11:31Z-1678031

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell4-sc-2013-08-14T09:11:32Z-1678032

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell5-sc-2013-08-14T09:11:34Z-1678033

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell6-sc-2013-08-14T09:11:35Z-1678034

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell7-sc-2013-08-14T09:11:36Z-1678035

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell8-sc-2013-08-14T09:11:38Z-1678036

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell9-sc-2013-08-14T09:11:39Z-1678037

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell10-sc-2013-08-14T09:11:40Z-1678038

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell11-sc-2013-08-14T09:11:41Z-1678039

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell12-sc-2013-08-14T09:11:42Z-1678040

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell13-sc-2013-08-14T09:11:43Z-1678041

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell14-sc-2013-08-14T09:11:45Z-1678042

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell15-sc-2013-08-14T09:11:46Z-1678043

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell16-sc-2013-08-14T09:11:47Z-1678044

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell17-sc-2013-08-14T09:11:48Z-1678045

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell18-sc-2013-08-14T09:11:49Z-1678046

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell19-sc-2013-08-14T09:11:50Z-1678047

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell20-sc-2013-08-14T09:11:52Z-1678048

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell21-sc-2013-08-14T09:11:52Z-1678049

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell22-sc-2013-08-14T09:11:53Z-1678050

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell23-sc-2013-08-14T09:11:54Z-1678051

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell24-sc-2013-08-14T09:11:56Z-1678052

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell25-sc-2013-08-14T09:11:57Z-1678053

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell26-sc-2013-08-14T09:11:58Z-1678054

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell27-sc-2013-08-14T09:11:59Z-1678055

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell28-sc-2013-08-14T09:12:00Z-1678056

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell29-sc-2013-08-14T09:12:01Z-1678057

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell30-sc-2013-08-14T09:12:02Z-1678058

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell31-sc-2013-08-14T09:12:03Z-1678059

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell32-sc-2013-08-14T09:12:04Z-1678060

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell33-sc-2013-08-14T09:12:05Z-1678061

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell34-sc-2013-08-14T09:12:08Z-1678062

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell35-sc-2013-08-14T09:12:09Z-1678063

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell36-sc-2013-08-14T09:12:10Z-1678064

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: OS_25_cell37-sc-2013-08-14T09:12:11Z-1678065

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell38-sc-2013-08-14T09:12:12Z-1678066

text: house mouse

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text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: public

text: OS_25_cell39-sc-2013-08-14T09:12:13Z-1678067

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: OS_25_cell40-sc-2013-08-14T09:12:14Z-1678068

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell41-sc-2013-08-14T09:12:15Z-1678069

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell42-sc-2013-08-14T09:12:16Z-1678070

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell43-sc-2013-08-14T09:12:18Z-1678071

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell44-sc-2013-08-14T09:12:19Z-1678072

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell45-sc-2013-08-14T09:12:21Z-1678073

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell46-sc-2013-08-14T09:12:22Z-1678074

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell47-sc-2013-08-14T09:12:23Z-1678075

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell48-sc-2013-08-14T09:12:24Z-1678076

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell49-sc-2013-08-14T09:12:25Z-1678077

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell50-sc-2013-08-14T09:12:26Z-1678078

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell51-sc-2013-08-14T09:12:28Z-1678079

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell52-sc-2013-08-14T09:12:29Z-1678080

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell53-sc-2013-08-14T09:12:31Z-1678081

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell54-sc-2013-08-14T09:12:32Z-1678082

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell55-sc-2013-08-14T09:12:33Z-1678083

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: OS_25_cell56-sc-2013-08-14T09:12:34Z-1678084

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell57-sc-2013-08-14T09:12:35Z-1678085

text: house mouse

text: Mus musculus

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell58-sc-2013-08-14T09:12:36Z-1678086

text: house mouse

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text: Mus musculus


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text: Mus Musculus

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text: OS_25_cell59-sc-2013-08-14T09:12:37Z-1678087

text: house mouse

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text: Mus musculus


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text: Mus Musculus

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text: OS_25_cell60-sc-2013-08-14T09:12:38Z-1678088

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: OS_25_cell61-sc-2013-08-14T09:12:39Z-1678089

text: house mouse

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text: Mus musculus


text: Embryonic

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text: Mus Musculus

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text: OS_25_cell62-sc-2013-08-14T09:12:40Z-1678090

text: house mouse

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text: OS_25_cell63-sc-2013-08-14T09:12:41Z-1678091

text: house mouse

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text: Mus musculus


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text: OS_25_cell64-sc-2013-08-14T09:12:42Z-1678092

text: house mouse

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text: Mus musculus


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text: Mus Musculus

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text: house mouse

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text: Mus musculus


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text: OS_25_cell66-sc-2013-08-14T09:12:44Z-1678094

text: house mouse

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text: house mouse

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text: 46c_day6_cell19-sc-2013-08-14T09:15:09Z-1678239

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell20-sc-2013-08-14T09:15:10Z-1678240

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell21-sc-2013-08-14T09:15:10Z-1678241

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell22-sc-2013-08-14T09:15:11Z-1678242

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell23-sc-2013-08-14T09:15:12Z-1678243

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell24-sc-2013-08-14T09:15:13Z-1678244

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell25-sc-2013-08-14T09:15:14Z-1678245

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell26-sc-2013-08-14T09:15:15Z-1678246

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell27-sc-2013-08-14T09:15:16Z-1678247

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell28-sc-2013-08-14T09:15:17Z-1678248

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell29-sc-2013-08-14T09:15:18Z-1678249

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell30-sc-2013-08-14T09:15:19Z-1678250

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell31-sc-2013-08-14T09:15:20Z-1678251

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell32-sc-2013-08-14T09:15:22Z-1678252

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell33-sc-2013-08-14T09:15:22Z-1678253

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell34-sc-2013-08-14T09:15:24Z-1678254

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell35-sc-2013-08-14T09:15:25Z-1678255

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell36-sc-2013-08-14T09:15:25Z-1678256

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell37-sc-2013-08-14T09:15:26Z-1678257

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell38-sc-2013-08-14T09:15:27Z-1678258

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell39-sc-2013-08-14T09:15:28Z-1678259

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell40-sc-2013-08-14T09:15:29Z-1678260

text: house mouse

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text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell41-sc-2013-08-14T09:15:30Z-1678261

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell42-sc-2013-08-14T09:15:31Z-1678262

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell43-sc-2013-08-14T09:15:32Z-1678263

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell44-sc-2013-08-14T09:15:33Z-1678264

text: house mouse

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text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

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text: 46c_day6_cell45-sc-2013-08-14T09:15:34Z-1678265

text: house mouse

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text: Mus musculus


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text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell46-sc-2013-08-14T09:15:35Z-1678266

text: house mouse

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text: Mus musculus


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text: Mus Musculus

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text: 46c_day6_cell47-sc-2013-08-14T09:15:37Z-1678267

text: house mouse

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text: Mus Musculus

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text: 46c_day6_cell48-sc-2013-08-14T09:15:38Z-1678268

text: house mouse

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text: Mus Musculus

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text: 46c_day6_cell49-sc-2013-08-14T09:15:39Z-1678269

text: house mouse

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text: 46c_day6_cell50-sc-2013-08-14T09:15:40Z-1678270

text: house mouse

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text: 46c_day6_cell51-sc-2013-08-14T09:15:41Z-1678271

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text: house mouse

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text: 46c_day6_cell57-sc-2013-08-14T09:15:47Z-1678277

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text: 46c_day6_cell60-sc-2013-08-14T09:15:49Z-1678280

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text: 46c_day6_cell61-sc-2013-08-14T09:15:50Z-1678281

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text: 46c_day6_cell62-sc-2013-08-14T09:15:51Z-1678282

text: house mouse

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text: house mouse

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text: house mouse

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text: house mouse

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text: house mouse

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text: 46c_day6_cell69-sc-2013-08-14T09:15:58Z-1678289

text: house mouse

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text: Mus Musculus

text: SC

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text: 46c_day6_cell70-sc-2013-08-14T09:15:59Z-1678290

text: house mouse

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text: Mus Musculus

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text: 46c_day6_cell71-sc-2013-08-14T09:16:00Z-1678291

text: house mouse

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text: Mus Musculus

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text: 46c_day6_cell72-sc-2013-08-14T09:16:01Z-1678292

text: house mouse

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text: Mus Musculus

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text: house mouse

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text: Mus Musculus

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text: 46c_day6_cell74-sc-2013-08-14T09:16:03Z-1678294

text: house mouse

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text: Mus musculus


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text: Embryonic stem cell

text: Mus Musculus

text: SC

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text: 46c_day6_cell75-sc-2013-08-14T09:16:04Z-1678295

text: house mouse

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text: Mus musculus


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text: Mus Musculus

text: SC

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text: 46c_day6_cell76-sc-2013-08-14T09:16:05Z-1678296

text: house mouse

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text: Mus musculus


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text: Mus Musculus

text: SC

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text: 46c_day6_cell77-sc-2013-08-14T09:16:06Z-1678297

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell78-sc-2013-08-14T09:16:07Z-1678298

text: house mouse

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text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell79-sc-2013-08-14T09:16:08Z-1678299

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell80-sc-2013-08-14T09:16:09Z-1678300

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell81-sc-2013-08-14T09:16:10Z-1678301

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell82-sc-2013-08-14T09:16:11Z-1678302

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell83-sc-2013-08-14T09:16:11Z-1678303

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell84-sc-2013-08-14T09:16:13Z-1678304

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell85-sc-2013-08-14T09:16:13Z-1678305

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell86-sc-2013-08-14T09:16:14Z-1678306

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell87-sc-2013-08-14T09:16:15Z-1678307

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell88-sc-2013-08-14T09:16:16Z-1678308

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell89-sc-2013-08-14T09:16:17Z-1678309

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell90-sc-2013-08-14T09:16:19Z-1678310

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell91-sc-2013-08-14T09:16:19Z-1678311

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell92-sc-2013-08-14T09:16:21Z-1678312

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell93-sc-2013-08-14T09:16:22Z-1678313

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell94-sc-2013-08-14T09:16:23Z-1678314

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell95-sc-2013-08-14T09:16:24Z-1678315

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: Embryonic

text: Embryonic stem cell

text: Mus Musculus

text: SC

text: public

text: 46c_day6_cell96-sc-2013-08-14T09:16:25Z-1678316

text: house mouse

text: Mus musculus

text: mRNA-seq Nextera library

text: Mus musculus


text: INRA-EPGV

text: public

text: DGVA

characteristic: broker name

text: tomato

text: Levovil line (Solanum lycopersicum)

characteristic: description

text: Solanum lycopersicum

text: Solanum lycopersicum

text: Levovil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: INRA-EPGV

text: public

text: DGVA

text: tomato

text: Stupicke line (Solanum lycopersicum)

text: Solanum lycopersicum

text: Solanum lycopersicum

text: Stupicke


characteristic: External Id

text: INRA-EPGV

text: public

text: DGVA

text: tomato

text: LA 0147 line (Solanum lycopersicum)

text: Solanum lycopersicum

text: Solanum lycopersicum

text: LA 0147


text: INRA-EPGV

characteristic: INSDC center name

text: public

text: DGVA

text: tomato

text: Ferum line (Solanum lycopersicum)

text: Solanum lycopersicum

text: Solanum lycopersicum

text: Ferum


text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC first public

text: public

text: __Spreadsheet__Formula_0x7f4471c6_-sc-2013-08-15T12:14:24Z-1680485

text: house mouse

text: Mus musculus

text: mESC

text: Mus musculus


text: female

text: Mus Musculus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: __Spreadsheet__Formula_0x26d75622_-sc-2013-08-15T12:14:40Z-1680486

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Oocyte

characteristic: sample description

text: Mus musculus


text: Mus Musculus

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: __Spreadsheet__Formula_0x2d1ed8cd_-sc-2013-08-15T12:14:58Z-1680487

text: house mouse

text: Mus musculus

text: Control

text: Mus musculus


text: public

text: 168 hour

characteristic: PAT cells culture time

characteristic: SRA accession

tag: Namespace:Department of Genetics, Evolution, and Environment & Cancer Institute, University College London

text: ArrayExpress

text: fission yeast

characteristic: common name

text: Protocols: Cells were grown in EMM medium at 32 degrees C and harvested from liquid cultures before or after removal of nitrogen from the media . Total RNA was isolated from S. pombe cells using a hot phenol method followed by phenol-chloroform extractions, and precipitation as described (Lyne et al., 2003, BMC Genomics 4, 27.). 10ug total RNA was used as starting material for strand-specific RNA-seq library preparation using an unreleased early version provided by Illumina of the Illumina TruSeq Small RNA Sample Prep Kit. Briefly: poly(A)+ RNA was enriched by two rounds of poly(dT) Sera-Mag magnetic beads purification. Enriched poly(A)+ RNA was then fragmented to an average size of ~200nt. Fragmented RNA was 3' de-phosphorylated with Antartic phosphatase and 5' phosphorylated with polynucleotide kinase this treatment prepares RNA fragments for subsequent ligation of Illumina RNA adaptors to their 5' and 3' ends using a 3' RNA ligase and a T4 RNA ligase, respectively. First strand cDNA was produced using a primer specific for the Illumina 3' adaptor. The library was finally amplified with 15 cycles of PCR using primers specific for the Illumina adaptors and purified using SPRI-beads (Agencourt, Beckman Coulter). Libraries size distributions and concentrations were determined on an Agilent Bioanalyzer

text: 972 h-

characteristic: genotype

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: mating type h minus

characteristic: sex

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Department of Genetics, Evolution, and Environment & Cancer Institute, University College London

text: public

text: 1 hour

text: 7 hour

text: 2 hour

text: 6 hour

text: 0 hour

text: 3 hour

text: 5 hour

text: 4 hour

text: 8 hour

text: ArrayExpress

characteristic: broker name

text: fission yeast

text: Protocols: Fission yeast pat1-114 cells (ade6-M210/ade-M216 pat1-114/pat1-114 h+/h+) were grown to mid-log phase at 25 degree C in Edinburgh Minimal Medium (EMM). Cells were washed twice in EMM without nitrogen source (EMM-N) and cultured for 12h in EMM-N at 25 degree C. The culture was then supplemented with 0.5g/L NH4Cl and shifted to 34 degree C. Samples were collected for RNA preparation just before, and every hour for 8 hours after shifting the culture to 34 degree C. Equal RNA amounts from each time-point were pooled and used for sequencing library preparation. Total RNA was isolated from S. pombe cells using a hot phenol method followed by phenol-chloroform extractions, and precipitation as described (Lyne et al., 2003, BMC Genomics 4, 27.). Equal amounts of RNA from each time point were pooled together. 10ug total RNA was used as starting material for strand-specific RNA-seq library preparation using an unreleased early version provided by Illumina of the Illumina TruSeq Small RNA Sample Prep Kit. Briefly: poly(A)+ RNA was enriched by two rounds of poly(dT) Sera-Mag magnetic beads purification. Enriched poly(A)+ RNA was then fragmented to an average size of ~200nt. Fragmented RNA was 3' de-phosphorylated with Antartic phosphatase and 5' phosphorylated with polynucleotide kinase this treatment prepares RNA fragments for subsequent ligation of Illumina RNA adaptors to their 5' and 3' ends using a 3' RNA ligase and a T4 RNA ligase, respectively. First strand cDNA was produced using a primer specific for the Illumina 3' adaptor. The library was finally amplified with 15 cycles of PCR using primers specific for the Illumina adaptors and purified using SPRI-beads (Agencourt, Beckman Coulter). Libraries size distributions and concentrations were determined on an Agilent Bioanalyzer

characteristic: description

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: mating type h plus

characteristic: title


E-MTAB-1629:pooled normal human testis

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-1629:pooled normal human testis

tag: Namespace:University of Science of Technology of China

characteristic: Submitter Id

text: 28 to 35 year

characteristic: age

text: ArrayExpress

text: human

text: Protocols: All the collections and usages of human tissues were approved by the Institutional Review Board at First Affiliated Hospital of Anhui Medical University. Testicular tissues were from three cadaver donors (aged 28 to 35 years) from the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China. Histological examination showed normal spermatogenesis in all of the three donators. Each patient has at least one healthy child. Total RNAs were extracted from the human testis using TRIzol (Invitrogen). These samples were then subjected to 15% (w/v) denaturing PAGE (polyacrylamide gel electrophoresis) and the small RNA fragments of 18-28 nt were isolated. The small RNA molecules were ligated to 5' and 3' adaptors sequentially and then converted to cDNA by reverse transcription followed by PCR amplifications

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: testis

characteristic: organism part

text: Homo sapiens

text: male

text: pooled normal human testis


characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital HF

text: ArrayExpress

characteristic: cell line

text: immortalized mesenchymal stem cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Minimum essential medium alpha with glutamax (Invitrogen), 10% fetal calf serum Growth medium (column N) supplemented with 67 uM ascorbic acid 2-phosphate (Sigma-Aldrich), 3.5 mM beta-glycerol phosphate (Sigma-Aldrich) and 10 nM dexamethasone (Sigma-Aldrich) Chromatin immunoprecipitation followed by phenol:chloroform:isoamylalcohol extraction of DNA Library prep TRUSeq, ChIP-Seq library prep Illumina.

text: 28-day differentiated

characteristic: developmental stage

text: day-0 undifferentiated

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state


name: E-MTAB-1828:Normal human osteoblasts (NHOst)

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital HF

text: public

text: ArrayExpress

characteristic: broker name

text: CC-2538 (product number)

text: normal osteoblast

text: human

text: Protocols: Clonetics Norman Human Osteoblast Cell System Clonetics Norman Human Osteoblast Cell System Chromatin immunoprecipitation followed by phenol:chloroform:isoamylalcohol extraction of DNA Library prep TRUSeq, ChIP-Seq library prep Illumina.

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

text: Normal human osteoblasts (NHOst)

characteristic: title


characteristic: ENA-CHECKLIST

text: UU

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: HepG2 cells were cultured to confluence in RPMI 1640 medium supplemented with 10% FBS. Total RNA was prepared using the TRIzol-chlorophorm metod (Introvitrogen) according to the manufactures protocol. We used a BioAnalyzer (Agilent) to quantify the RNA integrity (RIN 9.8) before library construction.

text: hepatocellular carcinoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: UU

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: HepG2 cells were cultured to confluence in RPMI 1640 medium supplemented with 10% FBS. Cells were serum-starved over night (1 % FBS) before stimulation with TGF-b1 (final conc. 2.5 ng/ml in complete medium). TGF-b1 treated and control cells were harvested after 1 hour treatment. Total RNA was prepared using the TRIzol-chlorophorm metod (Introvitrogen) according to the manufactures protocol. We used a BioAnalyzer (Agilent) to quantify the RNA integrity (RIN 9.8) before library construction.

text: hepatocellular carcinoma

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: immortalized mesenchymal stem cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Minimum essential medium alpha with glutamax (Invitrogen), 10% fetal calf serum RNA was prepared from cells cultured in GM or DM by TriReagent (Life Technologies) according to the manufacturer's recommendation. Isolated RNA was dissolved in water and used as template for complementary DNA (cDNA) synthesis, microarray hybridization or RNA-Seq. Library prep TRUSeq, RNA-Seq library prep Illumina.

text: undifferentiated

characteristic: developmental stage

text: 28-day differentiated

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state


characteristic: External Id

text: Institut Gustave Roussy

text: public

tag: Namespace:Institut Gustave Roussy

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: The source material was bone marrow or blood cells from Chronic Lymphocytic Leukemia (CLL) patients. Mononuclear cells (MNC) are separated through a density gradient (Ficoll). From MNC, B cells are FACS sorted using the CD19 marker. CD19+ cells were pellet after sorting and the RNA was extracted.

characteristic: description

text: chronic lymphocytic leukemia

text: EGR2 wild type

characteristic: genotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Institut Gustave Roussy

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ArrayExpress

text: human

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: all brain cells

characteristic: ArrayExpress-CellType

text: Postnatal

characteristic: ArrayExpress-DevelopmentalStage

text: not yet visible

characteristic: ArrayExpress-DiseaseState

text: DNMT1floxed Nestin Cre+

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: neurological

characteristic: ArrayExpress-Phenotype

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: 129SV/Bl6 mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: m_RRBS_910_2-sc-2013-08-22T09:21:25Z-1683348

text: house mouse

text: Mus musculus

text: reduced representation bisulfite libraries from genomic total brain DNA

characteristic: sample description

text: Mus musculus

text: 129SV/Bl6 mixed

characteristic: strain


text: all brain cells

text: Postnatal

text: DNMT1 floxed

text: Brain

text: wildtype

text: female

text: Mus musculus

text: 129SV/Bl6 mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: m_RRBS_910_3-sc-2013-08-22T09:21:29Z-1683349

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: 129SV/Bl6 mixed


text: all brain cells

characteristic: ArrayExpress-CellType

text: Postnatal

characteristic: ArrayExpress-DevelopmentalStage

text: not yet visible

characteristic: ArrayExpress-DiseaseState

text: DNMT1floxed Nestin Cre+

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: neurological

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

text: 129SV/Bl6 mixed

text: SC

text: public

text: m_RRBS_910_4-sc-2013-08-22T09:21:30Z-1683350

text: house mouse

text: Mus musculus

text: Mus musculus

text: 129SV/Bl6 mixed


text: all brain cells

text: Postnatal

text: DNMT1 floxed

text: Brain

text: wildtype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

text: 129SV/Bl6 mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: m_RRBS_910_5-sc-2013-08-22T09:21:32Z-1683351

text: house mouse

text: Mus musculus

characteristic: sample description

text: Mus musculus

text: 129SV/Bl6 mixed


text: all brain cells

text: Postnatal

text: not yet visible

text: DNMT1floxed Nestin Cre+

text: Brain

text: neurological

text: male

text: Mus musculus

text: 129SV/Bl6 mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: m_RRBS_910_8-sc-2013-08-22T09:21:33Z-1683352

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus

text: 129SV/Bl6 mixed

characteristic: strain