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text: SL6_10_C10-sc-2013-02-21T15:17:49Z-1513960

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_F10-sc-2013-02-21T15:17:52Z-1513963

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_A11-sc-2013-02-21T15:17:55Z-1513966

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_D11-sc-2013-02-21T15:17:58Z-1513969

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_G11-sc-2013-02-21T15:18:00Z-1513972

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_B12-sc-2013-02-21T15:18:03Z-1513975

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_10_E12-sc-2013-02-21T15:18:06Z-1513978

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: RAT

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus


text: Humanitas Clinical and Research Center

text: public

tag: Namespace:Humanitas Clinical and Research Center

text: ArrayExpress

characteristic: broker name

text: cardiac myocyte

characteristic: cell type

text: house mouse

text: Protocols: 2-month-old male C57BL/6J mice were subjected under anesthesia to transverse aortic constriction for 1 week. Cardiomyocytes were isolated from the hearts at the end of perfusion with collagenase (collagenase type II). A pool of 8 libraries was made using 473 pg of total DNA. In each case, the DNA was added to the PCR mix containing in a final volume of 5.6 mL: PCR Gold Buffer (Applied Biosystems), 1 ; AmpliTaq Gold, 3000 U; ePCR primer 1, 40 nM; ePCR primer 2, 3 uM; deoxynucleotide mix, 3.5 mM each; MgCl2, 25 mM; 1.6 billion SOLiD sequencing beads (Applied Biosystems). The PCR mix was added to 50 mL SOLiD ePCR Tube containing 9 ml of oil phase and emulsified using Ultraturrax Tube Drive (IKA). The emulsion was dispensed into 96-well plate and amplified for 40 cycles in a 9700 PCR device (Applied Biosystems). After amplification, the emulsion was broken disrupted with butanol, beads were enriched for template positive beads, 3p-end extended and covalently attached onto sequencing slides. A pool of eight samples were deposited on one sequencing slide and sequenced using standard settings on the SOLiD system version 3.5 to produce 50 nucleotide long reads.

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

characteristic: strain or line

characteristic: title

type: has member


text: Danio rerio

characteristic: ArrayExpress-Species

text: TL/AB

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SL6_06_E7-sc-2013-02-21T15:11:11Z-1513554

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SL6_06_D8-sc-2013-02-21T15:11:17Z-1513561

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: Exome

characteristic: sample description

text: Danio rerio

text: TL/AB

characteristic: strain


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_C9-sc-2013-02-21T15:11:23Z-1513568

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_F9-sc-2013-02-21T15:11:26Z-1513571

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_B10-sc-2013-02-21T15:11:29Z-1513575

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_E10-sc-2013-02-21T15:11:32Z-1513578

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_A11-sc-2013-02-21T15:11:35Z-1513582

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_D11-sc-2013-02-21T15:11:38Z-1513585

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_C12-sc-2013-02-21T15:11:44Z-1513592

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_F12-sc-2013-02-21T15:11:47Z-1513595

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_B1-sc-2013-02-21T15:11:50Z-1513599

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_E1-sc-2013-02-21T15:11:53Z-1513602

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_H1-sc-2013-02-21T15:11:56Z-1513605

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_C2-sc-2013-02-21T15:11:59Z-1513608

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_F2-sc-2013-02-21T15:12:02Z-1513611

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_C11-sc-2013-02-21T15:13:15Z-1513680

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_F11-sc-2013-02-21T15:13:18Z-1513683

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_A12-sc-2013-02-21T15:13:21Z-1513686

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_D12-sc-2013-02-21T15:13:24Z-1513689

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_07_G12-sc-2013-02-21T15:13:27Z-1513692

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_C1-sc-2013-02-21T15:13:30Z-1513696

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_F1-sc-2013-02-21T15:13:33Z-1513699

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_A2-sc-2013-02-21T15:13:36Z-1513702

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_D2-sc-2013-02-21T15:13:39Z-1513705

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_G2-sc-2013-02-21T15:13:42Z-1513708

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_B3-sc-2013-02-21T15:13:45Z-1513711

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_E3-sc-2013-02-21T15:13:50Z-1513714

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_H3-sc-2013-02-21T15:13:53Z-1513717

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_C4-sc-2013-02-21T15:13:56Z-1513720

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_F4-sc-2013-02-21T15:13:59Z-1513723

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_A5-sc-2013-02-21T15:14:02Z-1513726

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_D5-sc-2013-02-21T15:14:05Z-1513729

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_G5-sc-2013-02-21T15:14:08Z-1513732

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_B6-sc-2013-02-21T15:14:12Z-1513735

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_E6-sc-2013-02-21T15:14:14Z-1513738

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_H6-sc-2013-02-21T15:14:17Z-1513741

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_C7-sc-2013-02-21T15:14:20Z-1513744

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_F7-sc-2013-02-21T15:14:23Z-1513747

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_A8-sc-2013-02-21T15:14:26Z-1513750

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_D8-sc-2013-02-21T15:14:29Z-1513753

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_G8-sc-2013-02-21T15:14:32Z-1513756

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_E9-sc-2013-02-21T15:14:39Z-1513762

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_H9-sc-2013-02-21T15:14:41Z-1513765

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_C10-sc-2013-02-21T15:14:44Z-1513768

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_F10-sc-2013-02-21T15:14:47Z-1513771

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_A11-sc-2013-02-21T15:14:50Z-1513774

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_D11-sc-2013-02-21T15:14:53Z-1513777

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_G11-sc-2013-02-21T15:14:56Z-1513780

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_B12-sc-2013-02-21T15:14:59Z-1513783

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_08_E12-sc-2013-02-21T15:15:02Z-1513786

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_A1-sc-2013-02-21T15:15:05Z-1513790

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_D1-sc-2013-02-21T15:15:08Z-1513793

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_G1-sc-2013-02-21T15:15:11Z-1513796

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_B2-sc-2013-02-21T15:15:13Z-1513799

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_E2-sc-2013-02-21T15:15:16Z-1513802

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_C3-sc-2013-02-21T15:15:22Z-1513808

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_F3-sc-2013-02-21T15:15:26Z-1513811

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_A4-sc-2013-02-21T15:15:28Z-1513814

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_D4-sc-2013-02-21T15:15:31Z-1513817

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_G4-sc-2013-02-21T15:15:35Z-1513820

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_B5-sc-2013-02-21T15:15:38Z-1513823

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_F8-sc-2013-02-21T15:11:19Z-1513563

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_06_B9-sc-2013-02-21T15:11:22Z-1513567

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5465650-sc-2013-02-14T11:56:06Z-1558302

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5465653-sc-2013-02-14T11:56:13Z-1558305

text: house mouse

text: Mus musculus

text: Mus musculus


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_H5-sc-2013-02-21T15:15:43Z-1513829

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_C6-sc-2013-02-21T15:15:46Z-1513832

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_F6-sc-2013-02-21T15:15:49Z-1513835

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_A7-sc-2013-02-21T15:15:52Z-1513838

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_D7-sc-2013-02-21T15:15:55Z-1513841

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_G7-sc-2013-02-21T15:15:58Z-1513844

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_B8-sc-2013-02-21T15:16:01Z-1513847

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_E8-sc-2013-02-21T15:16:04Z-1513850

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_H8-sc-2013-02-21T15:16:07Z-1513853

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_C9-sc-2013-02-21T15:16:10Z-1513856

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_F9-sc-2013-02-21T15:16:13Z-1513859

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_A10-sc-2013-02-21T15:16:16Z-1513862

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_09_D10-sc-2013-02-21T15:16:18Z-1513865

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

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text: zebrafish

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text: Danio rerio

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text: SC

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text: Danio rerio

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text: Danio rerio

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text: Danio rerio

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text: Danio rerio

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text: Danio rerio

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text: Mus Musculus

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text: house mouse

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text: Mus musculus


text: Mus Musculus

text: SC

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text: SC_MUSPA5478813-sc-2013-02-26T11:21:27Z-1571739

text: house mouse

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text: Mus musculus


text: Mus Musculus

text: SC

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text: SC_MUSPA5478819-sc-2013-02-26T11:21:34Z-1571745

text: house mouse

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text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5478822-sc-2013-02-26T11:21:37Z-1571748

text: house mouse

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text: Mus Musculus

text: SC

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text: SC_MUSPA5481779-sc-2013-02-26T11:21:41Z-1574733

text: house mouse

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text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5481782-sc-2013-02-26T11:21:45Z-1574736

text: house mouse

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text: Mus musculus

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text: RNA from Bone of a biological replicate. GIF wildtype. Background C56BL-6J

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characteristic: External Id

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characteristic: Submitter Id

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tag: Namespace:Cambridge Research Institute

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characteristic: broker name

text: Norway rat

text: Protocols: doi:10.1016/j.ymeth.2009.03.001; Liver material was crosslinked in 1% formaldehyde for 20 minutes followed by a 10 minute incubation in 500 mM glycine buffer to neutralize the formaldehyde. Samples were rinsed with PBS and frozen until use. doi:10.1016/j.ymeth.2009.03.001; Crosslinked animal livers were dounce homogenized into a single cell suspension and rinsed with ice cold PBS. Each pellet of cross-linked material was resuspended in 10 ml of LB1 (50 mM Hepes-KOH, pH 7.5; 140 mM NaCl; 1mM EDTA; 10% Glycerol; 0.5% NP-40 or Igepal CA-630; 0.25% Triton X-100) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees C. Pellets were then rinsed in 10 ml of LB2 (10 mM Tris-HCL, pH8.0; 200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees C. The nuclei preparation was resuspended in 3 ml LB3 (10 mM Tris-HCl, pH 8; 100 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 0.1% Na-Deoxycholate; 0.5% N-lauroylsarcosine). Chromatin in the nuclei fraction was fragmented to an average length of 300 bp by sonication. After sonication 300 microlitreof 10% Triton X-100 was added to the 3 ml of sonicated lysate and debris was removed by centrifugation at 20,000 x rcf for 10 minutes at 4 degrees C. doi:10.1016/j.ymeth.2009.03.001; Crosslinked livers were dounce homogenized into a single cell suspension and rinsed with ice cold PBS. Each pellet of cross-linked material was resuspended in 10 ml of LB1 (50 mM Hepes-KOH, pH 7.5; 140 mM NaCl; 1mM EDTA; 10% Glycerol; 0.5% NP-40 or Igepal CA-630; 0.25% Triton X-100) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees Celcius. Pellets were then rinsed in 10 ml of LB2 (10 mM Tris-HCL, pH8.0; 200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees Celcius. The nuclei preparation was resuspended in 3 ml LB3 (10 mM Tris-HCl, pH 8; 100 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 0.1% Na-Deoxycholate; 0.5% N-lauroylsarcosine). Chromatin in the nuclei fraction was fragmented to an average length of 300 bp by sonication. After sonication 300ul of 10% Triton X-100 was added to the 3 ml of sonicated lysate and debris was removed by centrifugation at 20,000 x rcf for 10 minutes at 4 degrees Celcius. After 'chromatin_prep', 50ul of cell lysate from each sonication was removed as the input control DNA that was then processed as outlined in the 'chip_seq' protocol. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA version 2.2) with the following modifications. The ChIP-enriched DNA and input DNA were not further fragmented. After end-repair and addition of an A base to the 3-prime ends, the adapters were ligated to the ends of the DNA Fragments using 2ul of fourtyfold diluted Adapter oligo mix in a total reaction volume of 25ul. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5ul of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2% agarose gel and a gel slice containing the 200-300 bp fragments was excised.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: individual

text: Rattus norvegicus

text: Rattus norvegicus

text: liver

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cambridge Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: dog

text: adult

text: Canis lupus familiaris

text: Canis familiaris

text: liver

text: Canis lupus familiaris

text: male


text: Cambridge Research Institute

text: public

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: doi:10.1016/j.ymeth.2009.03.001; Liver material was crosslinked in 1% formaldehyde for 20 minutes followed by a 10 minute incubation in 500 mM glycine buffer to neutralize the formaldehyde. Samples were rinsed with PBS and frozen until use. doi:10.1016/j.ymeth.2009.03.001; Crosslinked animal livers were dounce homogenized into a single cell suspension and rinsed with ice cold PBS. Each pellet of cross-linked material was resuspended in 10 ml of LB1 (50 mM Hepes-KOH, pH 7.5; 140 mM NaCl; 1mM EDTA; 10% Glycerol; 0.5% NP-40 or Igepal CA-630; 0.25% Triton X-100) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees C. Pellets were then rinsed in 10 ml of LB2 (10 mM Tris-HCL, pH8.0; 200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees C. The nuclei preparation was resuspended in 3 ml LB3 (10 mM Tris-HCl, pH 8; 100 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 0.1% Na-Deoxycholate; 0.5% N-lauroylsarcosine). Chromatin in the nuclei fraction was fragmented to an average length of 300 bp by sonication. After sonication 300 microlitreof 10% Triton X-100 was added to the 3 ml of sonicated lysate and debris was removed by centrifugation at 20,000 x rcf for 10 minutes at 4 degrees C. doi:10.1016/j.ymeth.2009.03.001; Crosslinked livers were dounce homogenized into a single cell suspension and rinsed with ice cold PBS. Each pellet of cross-linked material was resuspended in 10 ml of LB1 (50 mM Hepes-KOH, pH 7.5; 140 mM NaCl; 1mM EDTA; 10% Glycerol; 0.5% NP-40 or Igepal CA-630; 0.25% Triton X-100) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees Celcius. Pellets were then rinsed in 10 ml of LB2 (10 mM Tris-HCL, pH8.0; 200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 degrees Celcius. The nuclei preparation was resuspended in 3 ml LB3 (10 mM Tris-HCl, pH 8; 100 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 0.1% Na-Deoxycholate; 0.5% N-lauroylsarcosine). Chromatin in the nuclei fraction was fragmented to an average length of 300 bp by sonication. After sonication 300ul of 10% Triton X-100 was added to the 3 ml of sonicated lysate and debris was removed by centrifugation at 20,000 x rcf for 10 minutes at 4 degrees Celcius. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA version 2.2) with the following modifications. The ChIP-enriched DNA and input DNA were not further fragmented. After end-repair and addition of an A base to the 3-prime ends, the adapters were ligated to the ends of the DNA Fragments using 2ul of fourtyfold diluted Adapter oligo mix in a total reaction volume of 25ul. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5ul of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2% agarose gel and a gel slice containing the 200-300 bp fragments was excised.

text: adult

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: male


characteristic: ENA-FIRST-PUBLIC

text: Cambridge Research Institute

text: public

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

text: adult

text: mmlBob

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male

text: mmlBob


text: OB+OC

characteristic: ArrayExpress-CellType

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Bone

characteristic: ArrayExpress-OrganismPart

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: 6 weeks

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Primary macrophages

text: Wildtype

text: Bone marrow

text: WT

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

text: Mus musculus

text: 24 hours

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: RNA from BMDM

text: Mus musculus

text: Mus musculus


text: Primary macrophages

text: Bone marrow

text: KO

text: male

text: Mus musculus

text: Mus musculus

text: 24 hours

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: RNA from BMDM

text: Mus musculus

text: Mus musculus


text: Primary macrophages

characteristic: ArrayExpress-CellType

text: 10 ng/ml

characteristic: ArrayExpress-Dose

characteristic: ArrayExpress-Genotype

text: Bone marrow

characteristic: ArrayExpress-OrganismPart

text: KO

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: Mus musculus

text: 24 hours

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA from BMDM

text: Mus musculus

text: Mus musculus


text: Primary macrophages

text: 100 nM

text: Bone marrow

text: KO

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: 24 hours

characteristic: ArrayExpress-TimePoint

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA from BMDM

characteristic: sample description

text: Mus musculus

text: Mus musculus

characteristic: strain


text: Mus Musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SC_MUSPA5478803-sc-2013-02-26T11:21:14Z-1571729

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

characteristic: SRA accession

text: SC_MUSPA5478809-sc-2013-02-26T11:21:22Z-1571735

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mus Musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: SC_MUSPA5478812-sc-2013-02-26T11:21:26Z-1571738

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5478815-sc-2013-02-26T11:21:29Z-1571741

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: SC_MUSPA5481778-sc-2013-02-26T11:21:40Z-1574732

text: house mouse

text: Mus musculus

text: Mus musculus


text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

text: SC

text: public

text: m_5mC_Neuron_1-sc-2013-03-19T13:56:07Z-1599114

text: house mouse

text: Mus musculus

text: gDNA from FACS sorted neurons

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Cambridge Research Institute

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: External Id

text: Cambridge Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Cambridge Research Institute

text: public

text: ArrayExpress

text: Rhesus monkey

text: adult

text: mmlBlues

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male

text: mmlBlues


text: Cambridge Research Institute

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male


text: Cambridge Research Institute

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

characteristic: individual

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male


text: Medical University of Gdansk

text: public

tag: Namespace:Medical University of Gdansk

text: ArrayExpress

text: leucocytes

characteristic: cell type

text: human

text: Protocols: Peripheral blood was collected into anticoagulant containing tubes and stored at ambient temperature until DNA extraction. DNA extraction was carried out with QuickGene-610L instrument (AUTOGEN; http://www.autogen.com/assets/pdf/QG-610L%20Brochure.pdf) using QuickGene DNA whole blood kit L (catalog number : DB-L, version 2.0) according to manufacturer's protocol. Custom SureSelect target enrichment library (Design ID: 0371891) was designed using SureDesign online tool (https://earray.chem.agilent.com/suredesign/, Agilent Technologies, Santa Clara, CA). The library targets exonic and non-coding evolutionary conserved elements within previously defined linkage intervals or regions of interest on chromosome 22. The library also includes the entire genomic sequence of the SMARCB1, NF2, CABIN1 genes. In total, the library covers 3.72 Mb of genomic reference sequence. Target capture was carried out according to manufacturer's protocol (Agilent Technologies, Santa Clara, CA).

text: schwannomatosis

characteristic: disease state

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC_DSCC15506258-sc-2013-03-21T10:41:12Z-1600180

text: house mouse

text: Mus musculus

text: Tumour

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Mouse

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC_ALLPax55490707-sc-2013-03-21T15:01:09Z-1584608

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mouse

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_ALLPax55490708-sc-2013-03-21T15:01:10Z-1584609

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490711-sc-2013-03-21T15:01:14Z-1584612

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490713-sc-2013-03-21T15:01:17Z-1584614

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490714-sc-2013-03-21T15:01:18Z-1584615

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490716-sc-2013-03-21T15:01:20Z-1584617

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490717-sc-2013-03-21T15:01:22Z-1584618

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490719-sc-2013-03-21T15:01:25Z-1584620

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490720-sc-2013-03-21T15:01:26Z-1584621

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490722-sc-2013-03-21T15:01:29Z-1584623

text: house mouse

text: Mus musculus

text: Mus musculus


E-SYBR-2:BB1533 rep2

text: EMBL-EBI

text: public

text: E-SYBR-2:BB1533 rep2

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: Fungal cell preparation: 5 S. cerevisiae strains were grown at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid YPD medium (1%, w/v yeast extract, 2%, w/v peptone and 2% w/v glucose). 5 C. albicans and parapsilosis strains were grown at at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid Saboraud medium (1%, w/v peptone and 2% w/v glucose). Cells were then harvested by centrifugation, washed twice with sterile water and resuspended in fresh RPMI 1640 complete medium. 4 A. fumigatus strains were grown at 37degC in solid Saboraud medium. Spores were then collected using PBS + 0.1% Tween and then washed twice with sterile water and resuspended in fresh RPMI 1640 complete. Human cell preparation: PBMCs were isolated from buffy coat blood sample from 6 healthy donors from the Transfusion Unit of the Careggi Hospital (Florence, Italy) by Ficoll-Hypaque density gradient centrifugation (Biochrom AG). Monocytes were isolated from low density PBMCs by magnetic enrichment with anti-CD14 beads (Miltenyi Biotec). Cells were cultured in RPMI complete medium (RPMI 1640, 10% w/v FBS, 1% w/v 200 mM glutamine, 1% w/v 50 mM sodium pyruvate, penicillin-streptomicin) in the presence of GM-CSF (800 U/ml) and recombinant IL-4 (1000 U/ml) for 6 days to allow dendritic cell (DC) differentiation (37degC, 5% CO2). Cell treatment: DCs were cultured for 4 hours with the different fungal strains at a stimuli:DC ratio of 5:1 (time= 4hr, 37degC, 5% CO2, complete RPMI medium). As controls, DCs or the single fungal strain were grown alone in the same experimental condition. RNA was extracted from cells using the standard TriZol protocol. For A. fumigatus containing samples, prior to TriZol extraction, fungal cells were disrupted by grinding in liquid nitrogen with pestle and mortar. RNA samples were then quantified, and their integrity checked using NanoChip BioAnalyzer technology (Agilent). For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: BB1533 rep2

characteristic: title


E-SYBR-2:DC  DC5412 + YA5

characteristic: External Id

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5412 + YA5

text: ArrayExpress

text: baker's yeast

text: DC DC5412

characteristic: individual

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5412 + YA5


text: Mouse

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC center name

text: public

text: SC_ALLPax55490723-sc-2013-03-21T15:01:31Z-1584624

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

characteristic: INSDC first public

text: public

text: SC_ALLPax55490725-sc-2013-03-21T15:01:34Z-1584626

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

characteristic: INSDC last update

text: public

text: SC_ALLPax55490726-sc-2013-03-21T15:01:35Z-1584627

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

characteristic: INSDC status

text: SC_ALLPax55490728-sc-2013-03-21T15:01:37Z-1584629

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

characteristic: SRA accession

text: SC_ALLPax55490729-sc-2013-03-21T15:01:38Z-1584630

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490731-sc-2013-03-21T15:01:40Z-1584632

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490732-sc-2013-03-21T15:01:41Z-1584633

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490734-sc-2013-03-21T15:01:43Z-1584635

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mouse

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC_ALLPax55490735-sc-2013-03-21T15:01:44Z-1584636

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_ALLPax55490737-sc-2013-03-21T15:01:46Z-1584638

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490738-sc-2013-03-21T15:01:48Z-1584639

text: house mouse

text: Mus musculus

text: Mus musculus


E-SYBR-2:DC  DC5618 + YA5

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5618 + YA5

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: DC DC5618

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5618 + YA5


text: male

characteristic: ArrayExpress-Sex

text: Homo sapiens

text: SC

text: public

text: SC_GMFUL5306370_LQ-sc-2013-03-15T13:26:10Z-1584578

text: human

text: Homo sapiens

text: Low input test

characteristic: sample description

text: Homo sapiens


text: Primordial germ cells

characteristic: ArrayExpress-CellType

text: TDG floxed/wt ; Tnap-Cre

characteristic: ArrayExpress-Genotype

text: male

text: mus musculus

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: E12_5PGCs_TDGflwt_TnapCre-sc-2013-03-15T10:41:07Z-1584000

text: house mouse

text: Mus musculus

text: Bisulfite sequencing

text: Mus musculus


text: Epiblast

characteristic: ArrayExpress-DevelopmentalStage

text: 3a+/-; 3b+/+

text: Epiblast

characteristic: ArrayExpress-OrganismPart

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

text: public

text: E7_5_Epi_Ctrl_1-sc-2013-03-18T14:51:19Z-1584799

text: house mouse

text: Mus musculus

text: RNA from E7.5 epiblast

text: Mus musculus


text: Epiblast

text: 3a-/-; 3b+/-

text: Epiblast

text: Mus Musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E7_5_Epi_3aKO_1-sc-2013-03-18T14:51:24Z-1584802

text: house mouse

text: Mus musculus

text: RNA from E7.5 epiblast

text: Mus musculus


text: EPC

text: 3a+/-; 3b+/-

text: EPC

text: Mus Musculus

text: SC

text: public

characteristic: SRA accession

text: E7_5_EPC_Ctrl_2-sc-2013-03-18T14:51:27Z-1584805

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: RNA from E7.5 EPC

text: Mus musculus


text: EPC

text: 3a-/-; 3b-/-

text: EPC

text: Mus Musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: E7_5_EPC_DKO_1-sc-2013-03-18T14:51:30Z-1584808

text: house mouse

text: Mus musculus

text: RNA from E7.5 EPC

text: Mus musculus


text: Liver

characteristic: ArrayExpress-CellType

text: Adult

text: Wildtype

text: Brain

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: m_5mC_Liver-sc-2013-03-19T13:56:03Z-1599113

text: house mouse

text: Mus musculus

text: gDNA from liver

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Glia

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

text: public

text: m_5mC_Glia_1-sc-2013-03-19T13:56:10Z-1599116

text: house mouse

text: Mus musculus

text: gDNA from FACS sorted glia

text: Mus musculus


text: Liver

text: Adult

text: Wildtype

text: Brain

text: wildtype

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: m_5hmC_Liver-sc-2013-03-19T13:56:13Z-1599119

text: house mouse

text: Mus musculus

text: gDNA from liver

text: Mus musculus


text: Glia

text: Adult

text: Wildtype

text: Brain

text: wildtype

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: m_5hmC_Glia_1-sc-2013-03-19T13:56:17Z-1599122

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: gDNA from FACS sorted glia

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508563-sc-2013-03-22T14:11:06Z-1602519

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508564-sc-2013-03-22T14:11:07Z-1602520

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508566-sc-2013-03-22T14:11:10Z-1602522

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508567-sc-2013-03-22T14:11:11Z-1602523

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508569-sc-2013-03-22T14:11:13Z-1602525

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508570-sc-2013-03-22T14:11:15Z-1602526

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508572-sc-2013-03-22T14:11:17Z-1602528

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508573-sc-2013-03-22T14:11:18Z-1602529

text: house mouse

text: Mus musculus

text: Mus musculus


E-SYBR-2:DC DC5618 + YB7

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5618 + YB7

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: Fungal cell preparation: 5 S. cerevisiae strains were grown at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid YPD medium (1%, w/v yeast extract, 2%, w/v peptone and 2% w/v glucose). 5 C. albicans and parapsilosis strains were grown at at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid Saboraud medium (1%, w/v peptone and 2% w/v glucose). Cells were then harvested by centrifugation, washed twice with sterile water and resuspended in fresh RPMI 1640 complete medium. 4 A. fumigatus strains were grown at 37degC in solid Saboraud medium. Spores were then collected using PBS + 0.1% Tween and then washed twice with sterile water and resuspended in fresh RPMI 1640 complete. Human cell preparation: PBMCs were isolated from buffy coat blood sample from 6 healthy donors from the Transfusion Unit of the Careggi Hospital (Florence, Italy) by Ficoll-Hypaque density gradient centrifugation (Biochrom AG). Monocytes were isolated from low density PBMCs by magnetic enrichment with anti-CD14 beads (Miltenyi Biotec). Cells were cultured in RPMI complete medium (RPMI 1640, 10% w/v FBS, 1% w/v 200 mM glutamine, 1% w/v 50 mM sodium pyruvate, penicillin-streptomicin) in the presence of GM-CSF (800 U/ml) and recombinant IL-4 (1000 U/ml) for 6 days to allow dendritic cell (DC) differentiation (37degC, 5% CO2). Cell treatment: DCs were cultured for 4 hours with the different fungal strains at a stimuli:DC ratio of 5:1 (time= 4hr, 37degC, 5% CO2, complete RPMI medium). As controls, DCs or the single fungal strain were grown alone in the same experimental condition. RNA was extracted from cells using the standard TriZol protocol. For A. fumigatus containing samples, prior to TriZol extraction, fungal cells were disrupted by grinding in liquid nitrogen with pestle and mortar. RNA samples were then quantified, and their integrity checked using NanoChip BioAnalyzer technology (Agilent). For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: DC DC5618

characteristic: individual

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: DC DC5618 + YB7

characteristic: title


E-SYBR-2:DC  DC6704 + YA5

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC6704 + YA5

text: ArrayExpress

text: baker's yeast

text: DC DC6704

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC6704 + YA5


E-SYBR-2:DC DC6704 + YB7

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC6704 + YB7

text: ArrayExpress

text: baker's yeast

text: DC DC6704

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC6704 + YB7


text: male

characteristic: ArrayExpress-Sex

text: Mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: SC_DSCC15487732-sc-2013-03-12T07:41:28Z-1581360

text: house mouse

text: Mus musculus

text: Tumour

characteristic: sample description

text: Mus musculus

characteristic: strain


text: male

text: Mouse

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SC_DSCC15487738-sc-2013-03-12T07:41:36Z-1581366

text: house mouse

text: Mus musculus

text: Tail

text: Mus musculus


text: female

text: Mouse

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: SC_DSCC15487741-sc-2013-03-12T07:41:40Z-1581369

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: Tail

text: Mus musculus


text: Hatching : 52 hpf Long pec ZFS:0000033 - pec fin ZFS:0000034

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_10_2_mutant-sc-2013-03-07T09:41:21Z-1580259

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 10 clutch2. A 5 base indexing sequence(CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph10

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_10_3_sibling-sc-2013-03-07T09:41:25Z-1580262

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 10 clutch3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph10

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_11_1_sibling-sc-2013-03-07T09:46:20Z-1580264

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 11 clutch1. A 5 base indexing sequence(AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_11_3_mutant-sc-2013-03-07T09:46:23Z-1580267

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 11 clutch3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

characteristic: sample description

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_11_4_sibling-sc-2013-03-07T09:46:27Z-1580270

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 11 clutch4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed

characteristic: strain


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_12_2_mutant-sc-2013-03-07T09:46:30Z-1580273

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 12 clutch2. A 5 base indexing sequence(CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

text: ZMP_phenotype_12_3_sibling-sc-2013-03-07T09:46:36Z-1580276

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 12 clutch3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: ZMP_phenotype_16_1_mutant-sc-2013-03-07T09:51:14Z-1580279

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_16_2_sibling-sc-2013-03-07T09:51:20Z-1580282

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: ZMP_phenotype_16_4_mutant-sc-2013-03-07T09:51:25Z-1580285

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_16_5_sibling-sc-2013-03-07T09:51:28Z-1580288

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_GMMAN5482164-sc-2013-03-07T10:01:15Z-1575164

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

characteristic: SRA accession

text: SC_GMMAN5482170-sc-2013-03-07T10:01:22Z-1575170

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482173-sc-2013-03-07T10:01:26Z-1575173

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482176-sc-2013-03-07T10:01:30Z-1575176

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482180-sc-2013-03-07T10:01:34Z-1575180

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482186-sc-2013-03-07T10:01:40Z-1575186

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482189-sc-2013-03-07T10:01:44Z-1575189

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482192-sc-2013-03-07T10:01:47Z-1575192

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482195-sc-2013-03-07T10:01:50Z-1575195

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482201-sc-2013-03-07T10:01:57Z-1575201

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482207-sc-2013-03-07T10:02:03Z-1575207

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482213-sc-2013-03-07T10:02:11Z-1575213

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482216-sc-2013-03-07T10:02:14Z-1575216

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482219-sc-2013-03-07T10:02:17Z-1575219

text: human

text: Homo sapiens

text: Homo sapiens


text: Wildtype

characteristic: ArrayExpress-Genotype

text: VNO

characteristic: ArrayExpress-OrganismPart

text: Wildtype

characteristic: ArrayExpress-Phenotype

text: mixed

text: Mouse

text: SC

text: public

text: newbornVNO3-sc-2013-03-14T09:46:45Z-1583320

text: house mouse

text: Mus musculus

text: RNA from VNO

text: Mus musculus


text: Wildtype

text: MOE

text: Wildtype

text: mixed

text: Mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: newbornMOE3-sc-2013-03-14T09:46:49Z-1583323

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from MOE

text: Mus musculus


text: Hatching : 52 hpf Long pec ZFS:0000033 - pec fin ZFS:0000034

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_10_1_mutant-sc-2013-03-07T09:41:16Z-1580257

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 10 clutch1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph10

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_11_2_mutant-sc-2013-03-07T09:46:21Z-1580265

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 11 clutch2. A 5 base indexing sequence(CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_11_3_sibling-sc-2013-03-07T09:46:25Z-1580268

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 11 clutch3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype12_2_sibling-sc-2013-03-07T09:46:31Z-1580274

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 12 clutch2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype12_4_mutant-sc-2013-03-07T09:46:37Z-1580277

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotypen 12 clutch4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_16_1_sibling-sc-2013-03-07T09:51:17Z-1580280

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_16_3_mutant-sc-2013-03-07T09:51:21Z-1580283

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed