E-MTAB-3599:Sample 4

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 4

tag: Namespace:Postdoctoral Fellow

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: H16N2 cells were cultured at 37 degree celsius with 5% carbon dioxide in F12 Medium supplemented with 5 ug/ml Insulin-transferrin-Sodium selenite, 1 mg/ml Hydrocortisone, 10 ng/mL EGF, 25 ug/ml Gentamicin, 2.5 ug/ml plasmocin, 0.3 ul/ml Ethanoamine, 10mM HEPES, 1 mg/ml Bovine Serum Albumin. 2% of Fetal Calf Serum was supplemented for 24 hours after re-platting. Cells were detached from the culture plates by treatment with 0.05% Trypsin/EDTA at 37 degree celsius for 3 minutes H16N2 cells were first transduced with empty vector or MIG retroviral vector carrying different EZH2 variants. After recovery from transduction, cells successfully transduced by MIG retroviral vector were sorted in flowcytometer based on GFP reporter. GFP positive cells were transduced a second time with shRNA targetting endogenous/human EZH2 in lentiviral vector. 10 ug/ml of puromycin was used to select for H16N2 cells that were transduced by the lentiviral vector. For expression analysis, total RNA from the cells was extract using QIAshredder (Qiagen) and RNeasy kit (Qiagen) according to manufacturer's protocol. Briefly, cells lysed in guanidine salt-containing buffer were centrifuged in QIAshredder for homogenization. RNA was precipitated by addition of 1 volume of 70% ethanol and captured in RNeasy column. RNeasy column with bound RNA was washed 3 times with buffers provided and eluted in RNase-free water. No DNAse I treatment was performed as the RNeasy columns do not bind genomic DNA. Library Preparation (Stranded mRNA-seq) The total RNA quality and RIN value was confirmed with a 2100 Bioanalyzer from Agilent using their RNA 6000 Nano chip. A high RIN value of 8 and above indicates that the total RNA quality was good and suitable for library preparation. Library preparation was done using a commercially available kit, Illumina® TruSeq Stranded mRNA LT Sample Prep Kit, following the manufacturer’s protocol. During the library construction, the enriched mRNA was fragmented to obtain inserts with fragment size of 120-200bp (a median size of 150 bp). The quality of the library was checked using both Agilent Bioanalyzer DNA1000 chip and KAPA quantification. A single peak in the expected region of ~280bp was observed indicating that the library was good and suitable for sequencing.

text: MIG was transduced for shRNA knockdown of endogenous EZH2

characteristic: genetic modification

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 4


E-MTAB-3599:Sample 18

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Postdoctoral Fellow

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3599:Sample 18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: VAVMT was transduced for shRNA knockdown of endogenous EZH2 and stably expresses murine EZH2 mutant with reduced VAV interaction

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 18

characteristic: title

type: has member


E-MTAB-3599:Sample 14

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 14

text: ArrayExpress

characteristic: cell line

text: human

text: H689A was transduced for shRNA knockdown of endogenous EZH2 and stably expresses murine enzymatically inactive EZH2

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 14


E-MTAB-3599:Sample 13

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 13

text: ArrayExpress

text: human

characteristic: genetic modification

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 13


E-MTAB-3599:Sample 3

characteristic: ENA-CHECKLIST

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 3

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 3


E-MTAB-3599:Sample 6

characteristic: ENA-FIRST-PUBLIC

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 6

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 6


E-MTAB-3599:Sample 2

characteristic: ENA-LAST-UPDATE

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 2

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 2


E-MTAB-3599:Sample 15

characteristic: External Id

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 15

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 15


E-MTAB-3599:Sample 9

text: Postdoctoral Fellow

characteristic: INSDC center name

text: public

text: E-MTAB-3599:Sample 9

text: ArrayExpress

text: human

text: EZH2 was transduced for shRNA knockdown of endogenous EZH2 and stably expresses murine wild-type EZH2

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 9


E-MTAB-3599:Sample 5

text: Postdoctoral Fellow

characteristic: INSDC first public

text: public

text: E-MTAB-3599:Sample 5

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 5


E-MTAB-3599:Sample 8

text: Postdoctoral Fellow

characteristic: INSDC last update

text: public

text: E-MTAB-3599:Sample 8

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 8


E-MTAB-3599:Sample 12

text: Postdoctoral Fellow

text: public

characteristic: INSDC status

text: E-MTAB-3599:Sample 12

text: ArrayExpress

text: human

text: NLS was transduced for shRNA knockdown of endogenous EZH2 and stably expresses murine cytosolic EZH2

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 12


E-MTAB-3599:Sample 7

text: Postdoctoral Fellow

text: public

characteristic: SRA accession

text: E-MTAB-3599:Sample 7

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 7


E-MTAB-3599:Sample 17

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 17

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 17


E-MTAB-3599:Sample 11

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 11

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 11


E-MTAB-3599:Sample 10

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 10

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 10


E-MTAB-3599:Sample 1

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 1

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 1


E-MTAB-3599:Sample 16

text: Postdoctoral Fellow

text: public

text: E-MTAB-3599:Sample 16

text: ArrayExpress

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 16


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ArrayExpress-CellType

text: RPMI1640_10% HiFBS_1% L-glut

characteristic: ArrayExpress-GrowthCondition

text: CTCF

characteristic: ArrayExpress-Immunoprecipitate

text: Normal

characteristic: ArrayExpress-Phenotype

text: Homo Sapien

text: SC

text: public

text: human

text: Homo sapiens

text: HEL9217 ChIP DNA

characteristic: sample description

text: Homo sapiens


text: RPMI1640_10% FBS_1% Glutamax_1% sodium pyruvate

text: CTCF

text: Normal

text: Homo Sapien

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: human

text: Homo sapiens

text: Meg01 ChIP DNA

text: Homo sapiens


text: RPMI1640_10% FBS_1% Glutamax_1% sodium pyruvate_10ng/ml rhGM-CSF

text: CTCF

text: Normal

text: Homo Sapien

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: F36P ChIP DNA

text: Homo sapiens


text: RPMI1640_10% FBS

text: CTCF

text: Normal

text: Homo Sapien

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: KU812 ChIP DNA

text: Homo sapiens


text: RPMI1640_10% HiFBS_1% L-glut

text: CTCF

text: Normal

text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: K562 ChIP DNA

text: Homo sapiens


text: IPK-Gatersleben

text: public

text: domesticated barley

text: Sequencing of barley BACs, that constitute the MTP of chromosome 5H

characteristic: description

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

characteristic: title


text: University of Cambridge

text: public

tag: Namespace:University of Cambridge

text: ArrayExpress

characteristic: broker name

text: thale cress

text: graft of shoot/root C24/Col Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer’s instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).

characteristic: ecotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

characteristic: organism part

text: Arabidopsis thaliana


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root C24/C24 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer’s instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root Col/Col Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer’s instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).

text: Col/Col

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root Col/Col Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. RNA-seq libraries were prepared from total RNA extracted as previously described in Molnar et al (Science 2010, 328, 872-875). RNA-seq libraries were generated with the ScriptSeq RNA-seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions.

text: Col/Col

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: thale cress

text: graft of shoot/root C24/Col Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. RNA-seq libraries were prepared from total RNA extracted as previously described in Molnar et al (Science 2010, 328, 872-875). RNA-seq libraries were generated with the ScriptSeq RNA-seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions.

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: Co/Col

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

characteristic: common name

text: graft of shoot/root dcl2,3,4/dcl2,3,4 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer’s instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root dcl2,3,4/dcl2,3,4 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. RNA-seq libraries were prepared from total RNA extracted as previously described in Molnar et al (Science 2010, 328, 872-875). RNA-seq libraries were generated with the ScriptSeq RNA-seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions.

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root C24/dcl2,3,4 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer’s instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana


text: University of Cambridge

text: public

text: ArrayExpress

text: thale cress

text: graft of shoot/root C24/dcl2,3,4 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. RNA-seq libraries were prepared from total RNA extracted as previously described in Molnar et al (Science 2010, 328, 872-875). RNA-seq libraries were generated with the ScriptSeq RNA-seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions.

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root structure

text: Arabidopsis thaliana

characteristic: title


text: public

text: E-MTAB-3597:Deckers_lab_May2015_STAT1_Y701F_untreated

tag: Namespace:Institute of Animal Breeding & Genetics, University of Veterinary Medicine Vienna

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

characteristic: cell type

text: house mouse

text: Protocols: Bone marrow derived macrophages (BMDM) were obtained by culture of mouse bone marrow in DMEM +10% FCS +10% L-cell-derived conditioned medium containing colony-stimulating factor 1 and antibiotics. The cells were used for the experiments after 10 days in culture. 15x106 cells were seeded on a 15 cm dish and treated with 250 U/mL of mouse recombinant IFNβ for 2 h or left untreated before harvesting. The ChIP was performed using antibody against mouse STAT1 (clone E-23, Santa Cruz). After treatment with IFNβ, formaldehyde was added to the cells to a final concentration of 1% and dishes were incubated for 15 minutes at 37°C. The reaction was quenched by adding 2.5 M glycine to a final concentration of 125 mM for 10 minutes. The fixed cells were washed with PBS and scraped. After centrifugation for 10 minutes at 600g cells were washed with wash I buffer (0.25%Triton X-100; 0.01M EDTA; 0.5 mM EGTA; 0.01 M Hepes) for 10 minutes at 4°C, followed by another centrifugation step. The pellet was resuspended in wash II buffer (0.2M NaCl; 1mM EDTA; 0.5 mM EGTA; 0.01 M Hepes) and kept for 10 minutes at 4°C. Cells were lysed over night with lysis buffer (1% SDS; 0.05M Tris pH 8; 0.01M EDTA) at 4°C. Cells were then sonicated 25 x 30 seconds using a Bioruptor sonicator. This resulted in chromatin fragments with an average length of 300 bp. Samples were centrifuged twice at 16.000g for 15 min and the supernatant was used for the subsequent steps. 1 µL of antibody was added to 25-30 µg of chromatin and rotated overnight at 4°C. Protein G Dynabeads (Invitrogen) were blocked over night in 0.1% BSA and on the next day added to the chromatin/antibody mixture and incubated for 3 hours at 4°C. Beads were then washed for 10 minutes in following buffers consecutively: RIPA buffer (0.15 M NaCl; 0.05 M Tris pH 8; 0.1% SDS; 0.5% NaDoc; 1% NP40), high salt buffer (0.5 M NaCl; 0.05 M Tris pH 8; 0.1% SDS; 1% NP40), LiCl buffer (0.25 M LiCl; 0.05 M Tris pH 8; 0.5% NaDoc; 1% NP40), and two times in TE buffer (1 M Tris pH 8, 0.5 M EDTA). Beads were then shaken in elution buffer (1 mL 10% SDS, 0.5 mL 1M NaHCO3, 50 µL 1M DTT in 5 mL of H2O) at room temperature for 40 minutes and 20 µl 4M NaCl was added to the eluted sample. Samples were incubated at 65°C overnight to reverse the cross-links. Samples were then treated with proteinase K (2 µl ptroteinase K, 8 µl EDTA 0.5 M pH 7.5 and 16 µl 1M TRIS pH 6.5) for 60 min at 55°C. 500 µl Phenol/Chloroform/Isoamyl alcohol (25/24/1, Sigma) was added to all samples and they were then centrifuged for 5 minutes at 16000g. The aqueous phase was used for DNA precipitation. 5-10 ng of DNA precipitate was used for the generation of sequencing libraries using the KAPA library preparation kit for Illumina systems. Libraries were quantified with a Bioanalyzer dsDNA 1000 assay kit (Agilent) and a Q-PCR NGS library quantification kit (KAPA).

text: Stat1 Y701F mutant

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

text: Institute of Animal Breeding & Genetics, University of Veterinary Medicine Vienna

text: public

text: E-MTAB-3597:Deckers_lab_May2015_STAT1_KO_untreated

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: Stat1 knock out

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-3597:Deckers_lab_May2015_STAT1_KO_IFNb

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: Stat1 knock out

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-3597:Deckers_lab_May2015_STAT1_Y701F_IFNb

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: Stat1 Y701F mutant

text: Mus musculus

text: Mus musculus

text: Mus musculus


text: 8.6 weeks

characteristic: ArrayExpress-Age

text: WT

characteristic: ArrayExpress-Genotype

text: Lin- bone marrow cells

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: Lin- bone marrow cells

characteristic: sample description

text: Mus musculus


text: 8.6 weeks

text: HEMI

text: Lin- bone marrow cells

text: male

text: Mus Musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: Lin- bone marrow cells

text: Mus musculus


text: 11.1 weeks

text: WT

text: Lin- bone marrow cells

text: male

text: Mus Musculus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Lin- bone marrow cells

text: Mus musculus


text: 11.1 weeks

text: double mutant

text: Lin- bone marrow cells

text: male

text: Mus Musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: Lin- bone marrow cells

text: Mus musculus


text: 11.2 weeks

characteristic: ArrayExpress-Age

text: WT

characteristic: ArrayExpress-Genotype

text: Lin- bone marrow cells

text: male

text: Mus Musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Lin- bone marrow cells

text: Mus musculus


text: 11.2 weeks

text: HET

text: Lin- bone marrow cells

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Lin- bone marrow cells

text: Mus musculus


characteristic: External Id

text: IPK-Gatersleben

text: public

text: domesticated barley

text: Sequencing of barley BACs, that constitute the MTP of chromosome 6H

characteristic: description

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen

text: 45.4 hour

characteristic: age

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: The cells were grown in yeast nitrogen base (YNB) without amino acids (ForMedium, Norfolk, UK) supplemented with 2% glucose, at a temperature of 30˚C, unless indicated differently. Precultures in flasks were shaken at 300 RPM. ). The quality and integrity of the RNA-samples was determined with a Nanodrop ND1000 spectrophotometer and analyzed on a RNA 6000 Lab-on-a-Chip using bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The cDNA libraries were generated by using the Illumina TruSeq mRNA-Seq Sample Prep Kit v2 (Illumina, San Diego, CA, USA). In short, mRNA was isolated from total RNA using the oligo-dT-magnetic beads, fragmented and cDNA synthesis was performed. The cDNA was ligated with the sequencing adapters and amplified by PCR. The quality of the amplified cDNA was measured with a DNA 1000 Lab-on-a-Chip. The fragment sizes ranged between 300 and 500 bp.

characteristic: mix

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 17.8 hour

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 72.3 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: public

text: 7.8 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 10.7 hour

text: ArrayExpress

text: baker's yeast

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 38.4 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: INSDC center name

text: public

text: 3 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: INSDC first public

text: public

text: 26.8 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 32.2 hour

characteristic: age

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 62.2 hour

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 22 hour

text: ArrayExpress

text: baker's yeast

characteristic: mix

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: INSDC last update

text: public

text: 53.3 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

characteristic: INSDC status

text: 5.3 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

characteristic: SRA accession

text: 1 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

characteristic: Submitter Id

text: 0 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: 14 hour

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: public

text: 45.4 hour

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-FIRST-PUBLIC

text: IPK-Gatersleben

text: public

text: domesticated barley

text: Sequencing of barley BACs, that constitute the MTP of chromosome 7H

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare


E-MTAB-3593:Sample 2

characteristic: ENA-LAST-UPDATE

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 2

tag: Namespace:FAU Division of Genetics

text: ArrayExpress

text: house mouse

text: Protocols: For generation of Meer cells primary hematopoietic precursor cells were isolated from the bone marrow of Meer mice that carry a knock-in of an ENL-ER (mutant estrogen receptor ligand binding domain) portion into the mouse Mll locus Takacova, S. et al. DNA damage response and inflammatory signaling limit the MLL-ENL-induced leukemogenesis in vivo. Cancer Cell 21, 517-31 (2012). CD117 (c-kit) positive cells were isolated by magnetic enrichment according to the instructions of the manufacturer (CD117 enrichment kit, Miltenyi Biotech, Bergisch-Gladbach, Germany) and initially plated in methocel (MethoCult M3234, StemCellTechnologies, Koln, Germany) to purge residual stroma cells that cannot grow in semisolid media. After two rounds of growth in methylcellulose cells were expanded in RPMI1640 supplemented with 10% FCS. All media contained 50ng/ml recombinant murine (rm) SCF, 10ng/ml of rm-IL3, IL6, GM-CSF (cytokines were bought from Miltenyi Biotech, Bergisch-Gladbach, Germany) and for activation of the estrogen receptor 100nM 4-hydroxytamoxifen (TAM, Sigma, Taufkirchen, Germany). Inactivation of MLL-ENL was achieved by washing out TAM keeping other media composition constant. ChIP was done exactly as described in (Milne, T.A., Zhao, K. & Hess, J.L. Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods Mol Biol 538, 409-23 (2009) with the exception that protein G-coupled paramagnetic beads from CellSignalingTechnologies (CST/NEB, Frankfurt, Germany) were used as precipitating agent. For ChIP of the estrogen receptor fusion a two-step protocol was used. After over-night incubation with anti-ER antibodies, anti-mouse immunoglobulins were added to enhance the signal. Antibodies and concentrations used : anti-ER (TE111.5D11) from ThermoScientific (Rockford, IL) 2.5ug per assay; anti-mouse IgG/M (H+L), ThermoScientific/Pierce 31198, 2.5ug per assay; anti-H3K79 dimethyl, AbCam 3594 polyclonal AB, 2ug; anti-RNApolII CTD serine2 phospho, ActiveMotif 61083, 10ug; anti-total RNApolII, ActiveMotif 39097, 2ug, anti-H3K4 di/trimethyl, ActiveMotif 39159 polyclonal, 3ul. ChIP Seq library construction with Illumina Kit according to the instructions of the manufacturer

text: gene knock in

characteristic: genetic modification

text: ENL-ER knock-in into Mll locus

characteristic: genotype

text: cell suspension culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 8 hour

characteristic: time

text: Sample 2


E-MTAB-3593:Sample 4

characteristic: External Id

text: FAU Division of Genetics

characteristic: INSDC center name

text: public

text: E-MTAB-3593:Sample 4

text: ArrayExpress

text: house mouse

characteristic: description

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: 24 hour

text: Sample 4

characteristic: title


E-MTAB-3593:Sample 12

text: FAU Division of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3593:Sample 12

text: ArrayExpress

characteristic: broker name

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 48 hour

text: Sample 12


E-MTAB-3593:Sample 22

text: FAU Division of Genetics

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3593:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 16 hour

text: Sample 22


E-MTAB-3593:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 1

text: ArrayExpress

text: house mouse

characteristic: common name

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 1


E-MTAB-3593:Sample 16

characteristic: ENA-LAST-UPDATE

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 16

text: ArrayExpress

text: house mouse

text: gene knock in

characteristic: genetic modification

text: ENL-ER knock-in into Mll locus

characteristic: genotype

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 16 hour

text: Sample 16


E-MTAB-3593:Sample 27

characteristic: External Id

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 27

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 72 hour

characteristic: time

text: Sample 27


E-MTAB-3593:Sample 7

text: FAU Division of Genetics

characteristic: INSDC center name

text: public

text: E-MTAB-3593:Sample 7

text: ArrayExpress

text: house mouse

characteristic: description

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 7


E-MTAB-3593:Sample 8

text: FAU Division of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3593:Sample 8

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 8

characteristic: title


E-MTAB-3593:Sample 15

text: FAU Division of Genetics

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3593:Sample 15

text: ArrayExpress

characteristic: broker name

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 8 hour

text: Sample 15


E-MTAB-3593:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 21

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 8 hour

text: Sample 21


E-MTAB-3593:Sample 10

characteristic: ENA-LAST-UPDATE

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 10

text: ArrayExpress

text: house mouse

characteristic: common name

text: gene knock in

characteristic: genetic modification

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 16 hour

text: Sample 10


E-MTAB-3593:Sample 20

characteristic: External Id

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 20

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

characteristic: genotype

text: cell suspension culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 20


E-MTAB-3593:Sample 18

text: FAU Division of Genetics

characteristic: INSDC center name

text: public

text: E-MTAB-3593:Sample 18

text: ArrayExpress

text: house mouse

characteristic: description

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 48 hour

characteristic: time

text: Sample 18


E-MTAB-3593:Sample 9

text: FAU Division of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3593:Sample 9

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: 8 hour

text: Sample 9


E-MTAB-3593:Sample 19

text: FAU Division of Genetics

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3593:Sample 19

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 72 hour

text: Sample 19

characteristic: title


E-MTAB-3593:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 11

text: ArrayExpress

characteristic: broker name

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 24 hour

text: Sample 11


E-MTAB-3593:Sample 5

characteristic: ENA-LAST-UPDATE

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 48 hour

text: Sample 5


E-MTAB-3593:Sample 26

characteristic: External Id

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 26

text: ArrayExpress

text: house mouse

text: gene knock in

characteristic: genetic modification

text: ENL-ER knock-in into Mll locus

characteristic: genotype

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 26


E-MTAB-3593:Sample 23

text: FAU Division of Genetics

characteristic: INSDC center name

text: public

text: E-MTAB-3593:Sample 23

text: ArrayExpress

text: house mouse

characteristic: description

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 24 hour

text: Sample 23


E-MTAB-3593:Sample 25

text: FAU Division of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3593:Sample 25

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 72 hour

characteristic: time

text: Sample 25


E-MTAB-3593:Sample 14

text: FAU Division of Genetics

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3593:Sample 14

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: 0 hour

text: Sample 14


E-MTAB-3593:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 24

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 48 hour

text: Sample 24

characteristic: title


E-MTAB-3593:Sample 13

characteristic: ENA-LAST-UPDATE

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 13

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 72 hour

text: Sample 13


E-MTAB-3593:Sample 6

characteristic: External Id

text: FAU Division of Genetics

text: public

text: E-MTAB-3593:Sample 6

text: ArrayExpress

text: house mouse

characteristic: common name

text: gene knock in

characteristic: genetic modification

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 72 hour

text: Sample 6


E-MTAB-3593:Sample 17

text: FAU Division of Genetics

characteristic: INSDC center name

text: public

text: E-MTAB-3593:Sample 17

text: ArrayExpress

text: house mouse

characteristic: description

text: gene knock in

text: ENL-ER knock-in into Mll locus

characteristic: genotype

text: cell suspension culture

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 24 hour

text: Sample 17


E-MTAB-3593:Sample 3

text: FAU Division of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3593:Sample 3

text: ArrayExpress

text: house mouse

text: gene knock in

text: ENL-ER knock-in into Mll locus

text: cell suspension culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 16 hour

text: Sample 3


text: Embryonic Stem Cell

characteristic: ArrayExpress-CellType

text: Serum + LIF

characteristic: ArrayExpress-GrowthCondition

text: Embryo

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Skin

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: PCR from earclip

characteristic: sample description

text: Mus musculus

characteristic: strain


characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Karolinska Institutet Department of Biosciences and Nutrition

text: 21 day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Lgr6-negative basal interfollicular epidermis cell

characteristic: cell type

text: house mouse

text: Protocols: Keratinocytes were isolated from three-week-old Lgr6-EGFP-CreETR2 mice as described previously (Jaks et al., 2008). Briefly, dorsal skin was harvested, cut in strips and digested in trypsin. Epidermal cells were peeled off the dermis and singularized by magnetic stirring. Cells from 2 - 3 mice were pooled per experiment. The collected cells were stained with AlexaFluor647-conjugated anti-CD49f antibody and PE-Cy7-conjugated anti-Sca-1 antibody (both from BD Biosciences), and filtered before sorting. Keratinocytes stained for CD49f, Sca-1 and 7-AAD were live sorted using a FACSAria (BD). Lgr6+ IFE cells were defined as the CD49fhigh, Sca-1high and EGFPhigh fraction. Lgr6+ HF cells were defined as the CD49fhigh+dim, Sca-1neg and EGFPhigh fraction. One hundred thousand cells were collected for each population. RNA was isolated from sorted keratinocytes by combining the RNA-containing aqueous phase extract using RNA-Bee (Amsbio) with the RNAaqueous-Micro Kit (Ambion). RNA integrity was measured using an Agilent 2100 Bioanalyzer Pico Chip or a 2200 TapeStation High Sensitivity R6K Screen Tape. The RNA integrity number of the used samples was higher than 8.5. The sequencing library was generated according to the STRT protocol, described in (Islam et al., 2012), using 1 ng total RNA as input for each sample. For each sample two technical replicates were sequenced (suffix _1 and _2). Islam, S., Kjällquist, U., Moliner, A., Zajac, P., Fan, J.B., Lönnerberg, P. and Linnarsson, S. (2012). Highly multiplexed and strand-specific single-cell RNA 5' end sequencing. Nat Protoc 7, 813-828.

characteristic: description

text: Lgr6cre-EGFP-IRES-CreERT2 heterozygous

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: dorsal skin

characteristic: organism part

text: CD49f hi/Sca1 hi/EGFP neg

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Karolinska Institutet Department of Biosciences and Nutrition

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 21 day

text: ArrayExpress

text: basal keratinocytes

text: house mouse

text: Lgr6cre-EGFP-IRES-CreERT2 heterozygous

text: Mus musculus

characteristic: organism

text: Mus musculus

text: dorsal skin

text: CD49f hi+dim

text: Mus musculus

text: male


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 21 day

text: ArrayExpress

text: Lgr6-dim basal interfollicular epidermis cell

text: house mouse

characteristic: common name

text: Lgr6cre-EGFP-IRES-CreERT2 heterozygous

text: Mus musculus

text: Mus musculus

text: dorsal skin

text: CD49f hi/Sca1 hi/EGFP dim

text: Mus musculus

text: male

characteristic: strain


text: Homo Sapien

characteristic: ArrayExpress-Species

text: Bisulphite methylation

characteristic: ArrayExpress-Treatment

text: SC

text: public

text: human

text: Homo sapiens

text: Bisulphite library

characteristic: sample description

text: Homo sapiens


text: Skin

characteristic: ArrayExpress-CellType

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: PCR from blastocyst

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IPK-Gatersleben

text: public

text: domesticated barley

text: Sequencing of barley BACs, that constitute the MTP of chromosome 1H

characteristic: description

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

characteristic: title


text: Segmentation:1-4 somites - Segmentation:5-9 somites : ZFS:0000023 - ZFS:0000024

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped embryo wild type for tfap2a, allele sa24445 and heterozygous for tfap2c, allele sa18857 ZMP phenotype 192 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph192.

text: Danio rerio

text: mixed


text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single genotyped embryo homozygous for tfap2a, allele sa24445 and wild type for tfap2c, allele sa18857 ZMP phenotype 192 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph192.

text: Danio rerio

text: mixed


text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped embryo heterozygous for tfap2a, allele sa24445 and wild type for tfap2c, allele sa18857 ZMP phenotype 192 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph192.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: ES cells

characteristic: ArrayExpress-CellType

text: Embryo

text: wt

characteristic: ArrayExpress-Genotype

text: Embryo

text: male

characteristic: ArrayExpress-Sex

text: mouse

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA wt replicate1

text: Mus musculus


text: ES cells

text: Embryo

characteristic: ArrayExpress-DevelopmentalStage

text: Embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: mouse

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA MBD3KO replicate1

text: Mus musculus


text: ES cells

text: Embryo

text: Embryo

text: male

text: mouse

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA Sall4KO replicate1

text: Mus musculus


text: ES cells

text: Embryo

text: wt

text: Embryo

text: male

text: mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: RNAseq libraries from rRNA depleted whole RNA wt replicate2

characteristic: sample description

text: Mus musculus


text: ES cells

characteristic: ArrayExpress-CellType

text: Embryo

characteristic: ArrayExpress-Genotype

text: Embryo

text: male

characteristic: ArrayExpress-Sex

text: mouse

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA MBD3KO replicate2

text: Mus musculus

characteristic: strain


text: ES cells

text: Embryo

characteristic: ArrayExpress-DevelopmentalStage

text: Embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: mouse

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA Sall4KO replicate2

text: Mus musculus


text: ES cells

text: Embryo

text: wt

text: Embryo

text: male

text: mouse

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA wt replicate3

text: Mus musculus


text: ES cells

text: Embryo

text: Embryo

text: male

text: mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: RNAseq libraries from rRNA depleted whole RNA MBD3KO replicate3

text: Mus musculus


text: ES cells

characteristic: ArrayExpress-CellType

text: Embryo

characteristic: ArrayExpress-Genotype

text: Embryo

text: male

characteristic: ArrayExpress-Sex

text: mouse

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA Sall4KO replicate3

characteristic: sample description

text: Mus musculus


text: Tumour

characteristic: ArrayExpress-Phenotype

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:Department of Molecular Neuroscience at the UCL Institute of Neurology. http://ulelab.info/

text: ArrayExpress

characteristic: broker name

text: HeLa

characteristic: cell line

text: human

text: Protocols: HeLa cells were cross-linked with 254nm UV. Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014. The 3 prime adapter sequence is AGATCGGAAG.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: Department of Molecular Neuroscience at the UCL Institute of Neurology. http://ulelab.info/

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: HeLa

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


E-MTAB-3625:siXRN1 / siSMG6

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-3625:siXRN1 / siSMG6

tag: Namespace:Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK.

text: ArrayExpress

text: HeLa

text: human

text: Protocols: HeLa cells were cultured overnight in 10 cm dishes and transiently transfected with 600pmol total siRNA following the protocol for RNAiMAX (Life Technologies). 24h after transfection, cells were split 1:8 in 10 cm dishes. 24 h later, cells again were transfected with 600 pmol (300 pmol each for double knockdowns) siRNA and incubated for 48 h. Poly(A)+ RNA was extracted from siRNA treated cells utilizing the magnetic mRNA isolation kit (New England Biolabs) 200 pmol of 5′ RNA linker, containing a recognition site for EcoP15I, was ligated to 3 µg poly(A)+ RNA using T4 RNA Ligase I. After DNAse I digest and RNA purification (RNA Clean & Concentrator Kit, Zymo Research) RT-PCR was performed using a linker oligo(dT)18-V. The cDNA was PCR amplified for 9 cycles, digested with EcoP15I overnight which cleaves 25 to 27 nt downstream of the recognition site, gel-purified and ligated to a 3′ dsDNA adaptor. After column purification of the ligation reaction, the ligated material was PCR-amplified for subsequent Illumina sequencing, using P3 and P5 primers and Accuprime Taq Polymerase (Life Technologies)

text: gene knock down

characteristic: genetic modification

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: siXRN1 / siSMG6


characteristic: ENA-FIRST-PUBLIC

text: Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK.

text: public

text: ArrayExpress

text: HeLa

text: human

text: gene knock down

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: HeLa

text: human

text: gene knock down

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: siLUC


characteristic: External Id

text: The University of Texas at Dallas

text: public

tag: Namespace:The University of Texas at Dallas

text: ArrayExpress

text: MCF 10A

text: human

text: Protocols: MCF10A, BT474, and SK-N-SH were obtained from American Type Culture Collection (ATCC) and cultured according to their recommended conditions. Cells were treated at 500 nM TSA (Trichostatin A), 5 μM SAHA (suberoylanilide hydroxamic acid), 500 nM JQ1, 500 nM FLAVO (flavopiridol), or 10 μM GEL (geldanamycin). 4 hr. treatments were done for elongation inhibitors TSA, SAHA, JQ1, and FLAVO, and GEL was applied for 4hr. and 15 min. (15 min. pre-treatment of GEL for combined GEL plus elongation inhibitor treatments). The 30 min. SAHA treatment for GRO-seq was the exception. Global run-on was performed as previously described (Kim, Y.J. et al. HDAC inhibitors induce transcriptional repression of high copy number genes in breast cancer through elongation blockade. Oncogene 32, 2828-35 (2013)), except detergent concentration was optimized, and libraries were multiplexed to conduct high-throughput sequencing in one lane. Cells were washed, swelled, and lysed in lysis buffer with a reduced amount of IGEPAL detergent to minimize nuclei damage while still being able to lyse cells (composition: 10 mM Tris-Cl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 10% glycerol, 2 U/mL SUPERasin (Ambion), and 0.25% IGEPAL). Nuclear run-on reactions were performed on fresh nuclei (no freezing), and nascent RNA was isolated. GRO-seq libraries was generated as previously (Kim, Y.J. et al. HDAC inhibitors induce transcriptional repression of high copy number genes in breast cancer through elongation blockade. Oncogene 32, 2828-35 (2013)) with following changes to allow multiplexing. Adaptors were ligated the same as before, however, TruSeq small RNA sample prep kit adapters and primers (Illumina) were used to make the samples suitable for multiplexing. The PCR reaction was run for 20-24 cycles for BT474, 21 cycles for MCF10A GRO-seq. The indexed libraries were purified by running on a 6% native polyacrylamide gel, and were cut out between 150 and 300 base pairs. The size profile of the isolated libraries was confirmed with a high-sensitivity DNA chip from Agilent according to manufacturer’s protocol and libraries were mixed in equal quantities based on qPCR quantitation and sent for sequencing.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: The University of Texas at Dallas

characteristic: INSDC center name

text: public

text: ArrayExpress

text: SK-N-SH

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: The University of Texas at Dallas

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: MYELOID CELLS

characteristic: ArrayExpress-CellType

text: AML

characteristic: ArrayExpress-Phenotype

text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

text: Homo sapiens

text: Total RNA extracted from MYELOID CELLS

characteristic: sample description

text: Homo sapiens


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: ES to NPC differentaion day 3

text: Mus musculus

characteristic: strain


text: male

text: Mus musculus

text: SC

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: ES to NPC differentaion day 6

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: ES to NPC differentaion day 0

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Department of Biochemistry and Molecular Biology University of Southern Denmark

text: ArrayExpress

characteristic: broker name

text: HeLa

characteristic: cell line

text: human

text: Protocols: T7-tagged hnRNP A1-expressing HeLa Cells were seeded in 10 cm dishes and induced with 1 µg/ml doxycycline (Sigma-Aldrich, www.sigmaaldrich.com) for 48 hours to 90% confluenceand before UV crosslinking. iCLIP was performed as described (iCLIP: protein-RNA interactions at nucleotide resolution by Huppertz et al. 2014, Methods, 65(3):274-87) with three biological replicates. Briefly, HeLa cells with stable inducible expression of T7-tagged hnRNP A1 were cultured in RPMI 1640 with L-Glutamine (Lonza, www.lonza.com) supplemented with streptomycin, penicillin and glutamine. Cells were seeded in 10 cm dishes and induced with 1 µg/ml doxycycline (Sigma-Aldrich, www.sigmaaldrich.com) for 48 hours before UV crosslinking. RNase Cocktail™ Enzyme Mix (Life Technologies, www.lifetechnologies.com), a mixture of RNaseA and RNaseT1 was used for RNA digestion. It was diluted 1:10, 1:200 and 1:2000. Protein G Dynabeads (Life Technologies) were coupled to T7-antibody (69522-4, Merck Millipore, www.merckmillipore.com/) and RNA-protein complexes were isolated and purified. cDNA was synthesized with Superscript II Reverse Transcriptase (Life Technologies). After PCR amplification, DNA fragments below 100 bp were removed by purification with Agencourt AMPure XP beads (Beckman Coulter, https://www.beckmancoulter.com).

characteristic: description

text: doxycycline-inducible T7-tagged hnRNP A1

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: Department of Biochemistry and Molecular Biology University of Southern Denmark

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: HeLa

text: human

text: Protocols: Hela cells 60% confluent were treated with siRNA (non-targeting D-001810-10-20, Dharmacon) or hnRNP A1 (L-008221-00, Dharmacon) Using Illumina's TruSeq Stranded Total RNA sample prep kit

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC last update

text: public

text: ArrayExpress

text: HeLa

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: ES to NPC differentaion day 1

characteristic: sample description

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The University of Texas at Dallas

text: public

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: BT474 cells were obtained from American Type Culture Collection (ATCC) and cultured according to their recommended conditions. Cells were treated at 5 μM SAHA (suberoylanilide hydroxamic acid) or 500 nM FLAVO (flavopiridol) for 4 hours. Chromatin was prepared and immunoprecipitated as previously described (Kim, Y.J., Cecchini, K.R. & Kim, T.H. Conserved, developmentally regulated mechanism couples chromosomal looping and heterochromatin barrier activity at the homeobox gene A locus. Proc Natl Acad Sci U S A 108, 7391-6 (2011)), except that 25% of the amount of chromatin was used for RNAP2 and acetyl ChIPs to reduce oversaturation of bead binding. ThruPLEX® DNA-seq Kit from Rubicon Genomics was used for multiplexed ChIP-seq library prep of BT474 chromatin. Indexed samples were quantitated with qPCR and mixed in equimolar amounts. Input sample was prepared with an Illumina DNA-seq kit.

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: The University of Texas at Dallas

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: INPUT


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Dept. of Tree Physiology and Forest Botany University of Gottingen

text: ArrayExpress

text: European beech

text: Protocols: A 100 cm^2 area of bark was peeled of a living tree. Developing xylem was scraped from the surface underneath and directly frozen in liquid nitrogen. Frozen tissue was ground under liquid nitrogen in a ball mill (Retsch, Haan, Germany). Tissue powder (500 mg) was used for RNA extraction after Chang et al. (1993) with minor modifications: the extraction buffer contained 2% b-mercaptoethanol and no spermindine. RNA was purified (RNeasy Mini Kit, Qiagen, Venlo, Netherlands). Total RNA yield and purity were determined spectrophotometrically (NanoDrop, Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). TruSeq RNA Library Preparation Kit (Illumina)

text: April

characteristic: harvest timepoint

characteristic: individual

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

characteristic: organism part

text: Calvoerde

characteristic: sampling site

text: Fagus sylvatica

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Dept. of Tree Physiology and Forest Botany University of Gottingen

text: public

characteristic: SRA accession

text: ArrayExpress

text: European beech

text: Protocols: A 100 cm^2 area of bark was peeled of a living tree. Developing xylem was scraped from the surface underneath and directly frozen in liquid nitrogen. Mint-2 Kit for cDNA preparation (Evrogen) & cDNA normalization TRIMMER kit (Evrogen) Frozen tissue was ground under liquid nitrogen in a ball mill (Retsch, Haan, Germany). Tissue powder (500 mg) was used for RNA extraction after Chang et al. (1993) with minor modifications: the extraction buffer contained 2% b-mercaptoethanol and no spermindine. RNA was purified (RNeasy Mini Kit, Qiagen, Venlo, Netherlands). Total RNA yield and purity were determined spectrophotometrically (NanoDrop, Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). TruSeq RNA Library Preparation Kit (Illumina)

text: August

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Goerde

text: Fagus sylvatica


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: European beech

characteristic: common name

text: August

text: Fagus sylvatica

characteristic: organism

text: Fagus sylvatica

text: developing xylem

text: Calvoerde

text: Fagus sylvatica


characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: European beech

characteristic: description

text: June

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Calvoerde

text: Fagus sylvatica


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: European beech

text: October

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Goerde

text: Fagus sylvatica


text: public

text: ArrayExpress

text: European beech

text: June

characteristic: harvest timepoint

characteristic: individual

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

characteristic: organism part

text: Goerde

text: Fagus sylvatica


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: European beech

text: April

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Calvoerde

characteristic: sampling site

text: Fagus sylvatica

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: SRA accession

text: ArrayExpress

text: European beech

text: October

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Calvoerde

text: Fagus sylvatica


characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: European beech

characteristic: common name

text: April

text: Fagus sylvatica

text: Fagus sylvatica

text: developing xylem

text: Goerde

text: Fagus sylvatica


characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: European beech

text: div.

text: blend

text: Fagus sylvatica

characteristic: organism

text: Fagus sylvatica

text: developing xylem

text: Calvoerde & Goerde

text: Fagus sylvatica


text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: Plasmodium falciparum

text: SC

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: iPS

characteristic: ArrayExpress-CellType

text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

characteristic: ENA-CHECKLIST

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

characteristic: description

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree C2 on July 18 in 2011

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree D on July 11 in 2011


characteristic: External Id

text: UPSC

text: public

characteristic: SRA accession

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree F2 on July 25 in 2011


text: UPSC

text: public

characteristic: Submitter Id

text: Norway spruce

characteristic: common name

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree J2 on June 27 in 2011


text: UPSC

characteristic: INSDC center name

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree M on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

characteristic: organism

text: Picea abies

text: Phloem/cambium sample collected from tree W on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree X on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/Cambium sample collected from tree V on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree M2 on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree A2 on May 16 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree A2 on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree B2 on May 16 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree B2 on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree C on January 23 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree C on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree D2 on July 25 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree D2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree E2 on July 25 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree E2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree F2 on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree F on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree G2 on February 21 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree G2 on July 18 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree G on August 8 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree G on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree H2 on July 18 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree H2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree I2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree I2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree I on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree I on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree J2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree J on September 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree K2 on June 27 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree K2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree K on September 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree L2 on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree L2 on June 27 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree L on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree M2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree M on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree N on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree N on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree O2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree O2 on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree O on January 23 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree P on February 21 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree P on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree Q on September 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree S on August 8 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree S on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree U on August 8 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree U on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree V on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree W on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree X on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree Y on May 16 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree Y on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree Z on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample collected from tree Z on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Phloem/cambium sample from tree O2 on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree A2 on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree A2 on May 16 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree A2 on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree B2 on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree B2 on May 16 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree B2 on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree C2 on February 21 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree C2 on July 18 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree C on January 23 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree C on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree D2 on July 25 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree D2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree D on January 23 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree D on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree E2 on July 25 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree E2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree F2 on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree F2 on July 25 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree F on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree G2 on February 21 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree G2 on July 18 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree G on August 8 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree G on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree H2 on July 18 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree H2 on March 27 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree I2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree I2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree I on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree I on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree J2 on June 27 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree J2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree J on June 13 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree J on September 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree K2 on June 27 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree K2 on November 16 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree K on June 13 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree L2 on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree L2 on June 27 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree L on June 13 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree L on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree M2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree M2 on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree M on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree M on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree N on December 14 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree N on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree O2 on June 20 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree O2 on October 17 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree O on January 23 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree O on July 4 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree S on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree U on May 2 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree V on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree V on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree W on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree W on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree X on August 22 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree X on May 11 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree Y on September 6 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree Z on April 24 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree Z on August 1 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree P on February 21 in 2012


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree P on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree Q on July 11 in 2011


text: UPSC

text: public

text: Norway spruce

text: Genotype Z4006 in Hissjo

text: Picea abies

text: Picea abies

text: Xylem sample collected from tree S on August 8 in 2011


text: NCGR

text: public

text: Japanese rice

text: PacBio SMRT sequencing of polyA selected sample from rice leaf

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group


text: eggs

characteristic: ArrayExpress-DevelopmentalStage

text: mixed

text: Haemonchus contortus

characteristic: ArrayExpress-Species

text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Pre-IVM

characteristic: sample description

text: Haemonchus contortus


text: eggs

text: mixed

text: Haemonchus contortus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: barber pole worm

text: Haemonchus contortus

text: Post-IVM

text: Haemonchus contortus


text: mixed

characteristic: ArrayExpress-Sex

text: Haemonchus contortus

text: MHco18 (UGA2004)

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: MHco18 (UGA2004)

characteristic: strain


text: male

text: Haemonchus contortus

text: ISE

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: ISE


text: Haemonchus contortus

text: UK Farm 95

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: UK Farm 95


text: Haemonchus contortus

text: ISE

text: SC

text: public

characteristic: Submitter Id

text: barber pole worm

characteristic: common name

text: Haemonchus contortus

text: Haemonchus contortus

text: ISE


text: Haemonchus contortus

text: MHco18 (UGA2004)

text: SC

characteristic: INSDC center name

text: public

text: barber pole worm

text: Haemonchus contortus

characteristic: organism

text: Haemonchus contortus

text: MHco18 (UGA2004)