text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G571


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NCGR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: sesame

characteristic: common name

text: Large collection of sesame germplasm

characteristic: description

text: Sesamum indicum

characteristic: organism

text: Sesamum indicum

text: Sesame varieties G572

characteristic: title


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G573


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G574


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G575


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G576


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G577


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G578


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G579


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G580


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G581


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G582


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G583


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G584


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G585


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G586


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G587


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G588


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G589


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G590


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G591


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G592


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G593


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G594


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G595


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G596


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G597


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G598


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G599


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G600


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G601


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G602


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G603


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G604


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G605


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G606


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G607


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G608


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G609


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G610


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G611


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G612


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G613


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G614


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G615


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G616


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G617


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G618


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G619


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G620


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G621


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G622


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G623


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G624


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G625


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G626


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G627


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G628


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G629


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G630


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G631


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G632


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G633


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G634


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G635


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G636


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G637


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G638


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G639


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G640


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G641


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G642


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G643


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G644


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G645


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G646


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G647


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G648


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G649


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G650


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G651


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G652


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G653


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G654


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G655


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G656


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G657


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G658


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G659


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G660


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G661


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G662


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G663


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G664


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G665


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G666


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G667


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G668


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G669


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G670


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G671


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G672


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G673


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G674


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G675


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G676


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G677


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G678


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G679


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G680


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G681


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G682


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G683


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G684


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G685


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G686


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G687


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G688


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G689


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G690


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G691


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G692


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G693


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G694


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G695


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G696


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G697


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G698


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G699


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G700


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G701


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G702


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G703


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G704


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G705


text: NCGR

text: public

text: sesame

text: Large collection of sesame germplasm

text: Sesamum indicum

text: Sesamum indicum

text: Sesame varieties G706


text: Boston Children's Hospital

text: public

tag: Namespace:Boston Children's Hospital

text: ArrayExpress

characteristic: broker name

text: pig

text: Protocols: mRNA library construction with Illumina TruSeq kit

text: Post-traumatic osteoarthritis

characteristic: disease

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa

type: has member


text: Boston Children's Hospital

text: public

text: ArrayExpress

text: pig

text: Intact

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa


E-MTAB-3198:AB2.2 NPC total polyA

text: CSHL

text: public

text: E-MTAB-3198:AB2.2 NPC total polyA

text: ArrayExpress

characteristic: cell line

text: neural progenitor cell

characteristic: cell type

text: whole cell

characteristic: cellular component

text: house mouse

text: Protocols: ESC colonies were maintained on gelatinized cell culture dishes, on a feeder layer of irradiated mouse embryonic fibroblasts in knock-out DMEM supplemented with 15 % fetal bovine serum, non-essential amino acids and 1,000 U/ml leukemia inhibiting factor. NPCs were maintained on gelatinized cell culture dishes in N2 expansion medium composed of 50:50 DMEM / F12: neurobasal medium, supplemented with 1x N2, 0.05x B-27, 50 ug /ml BSA (fraction V), 1ug / ml laminin, and 10 ng / ml each murine basic fibroblast growth factor and epidermal growth factor Total RNA was isolated using TRIzol reagent. For ASO knock-down studies, total RNA was collected using TRIzol and precipitated in the presence of 7.5 ug GlycoBlue (Ambion, Life Technologies). RNA integrity was assessed on the Agilent 2100 Bioanalyzer instrument and only RNA samples with integrity scores > 9.5 (median 10.0) were used. Stranded libraries for paired-end sequencing were prepared as described in Parkhomchuk, D., Borodina, T., Amstislavskiy, V., Banaru, M., Hallen, L., Krobitsch, S., Lehrach, H., and Soldatov, A. (2009). Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37, e123.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: AB2.2 NPC total polyA


E-MTAB-3198:AB2.2 ESC total polyA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSHL

text: public

text: E-MTAB-3198:AB2.2 ESC total polyA

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: AB2.2 ESC total polyA


E-MTAB-3198:ESC PAT14 ASO#3 Rep4

text: CSHL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3198:ESC PAT14 ASO#3 Rep4

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Protocols: ESC colonies were maintained on gelatinized cell culture dishes, on a feeder layer of irradiated mouse embryonic fibroblasts in knock-out DMEM supplemented with 15 % fetal bovine serum, non-essential amino acids and 1,000 U/ml leukemia inhibiting factor. NPCs were maintained on gelatinized cell culture dishes in N2 expansion medium composed of 50:50 DMEM / F12: neurobasal medium, supplemented with 1x N2, 0.05x B-27, 50 ug /ml BSA (fraction V), 1ug / ml laminin, and 10 ng / ml each murine basic fibroblast growth factor and epidermal growth factor For transfection of ASOs using nucleofection technology (Lonza), ESCs were harvested following soaking off of feeder cells for one hour, washed in D-PBS and passed through a 70 um nylon cell strainer. Cell count and viability was determined by trypan blue staining on a Countess automated cell counter. For each reaction, 5x104 viable cells were resuspended in P3 Primary Cell solution (Lonza), mixed with 2 uM control or 2 uM target-specific ASO and transferred to nucleocuvettes for nucleofection on a 4D-Nucleofector System (Lonza) using program code DC-100. Cells were subsequently transferred onto gelatinized cell culture plates containing pre-warmed and supplemented growth medium. Growth medium was changed once after 16 hours. Total RNA was isolated using TRIzol reagent. For ASO knock-down studies, total RNA was collected using TRIzol and precipitated in the presence of 7.5 ug GlycoBlue (Ambion, Life Technologies). RNA integrity was assessed on the Agilent 2100 Bioanalyzer instrument and only RNA samples with integrity scores > 9.5 (median 10.0) were used. Libraries were prepared from poly(A)+ selected RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina) according to the manufacturers instructions, except that final libraries were subject to size selection followed by gel purification and validation on the Agilent 2100 Bioanalyzer.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#3 Rep4


E-MTAB-3198:ESC PAT14 ASO#2 Rep1

text: CSHL

text: public

characteristic: SRA accession

text: E-MTAB-3198:ESC PAT14 ASO#2 Rep1

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#2 Rep1


E-MTAB-3198:ESC Control ASO Rep5

text: CSHL

text: public

text: E-MTAB-3198:ESC Control ASO Rep5

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep5

characteristic: title


E-MTAB-3198:ESC PAT14 ASO#2 Rep4

text: CSHL

text: public

text: E-MTAB-3198:ESC PAT14 ASO#2 Rep4

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: whole cell

characteristic: cellular component

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#2 Rep4


E-MTAB-3198:ESC Firre ASO#5 Rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#5 Rep2

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: ESC Firre ASO#5 Rep2


E-MTAB-3198:AB2.2 ESC nuclear polyA

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSHL

characteristic: INSDC center name

text: public

text: E-MTAB-3198:AB2.2 ESC nuclear polyA

text: ArrayExpress

text: embryonic stem cell

text: nucleus

text: house mouse

text: Protocols: Nuclear RNA fractions were obtained by resuspending cells to 2x107 / ml in ice-cold nuclei buffer (10 mM Tris pH 7.6, 10 mM NaCl, 2 mM MgCl2) supplemented with protease inhibitor cocktail and RNAse inhibitor. Cells were kept on ice for 10 minutes for hypotonic swelling prior to adding an equal volume of nuclei buffer containing 0.5% NP-40 for 5 minutes. Nuclei were collected, washed in nuclei buffer / 0.5% NP-40 and lysed in TRIzol. ESC colonies were maintained on gelatinized cell culture dishes, on a feeder layer of irradiated mouse embryonic fibroblasts in knock-out DMEM supplemented with 15 % fetal bovine serum, non-essential amino acids and 1,000 U/ml leukemia inhibiting factor. NPCs were maintained on gelatinized cell culture dishes in N2 expansion medium composed of 50:50 DMEM / F12: neurobasal medium, supplemented with 1x N2, 0.05x B-27, 50 ug /ml BSA (fraction V), 1ug / ml laminin, and 10 ng / ml each murine basic fibroblast growth factor and epidermal growth factor Total RNA was isolated using TRIzol reagent. For ASO knock-down studies, total RNA was collected using TRIzol and precipitated in the presence of 7.5 ug GlycoBlue (Ambion, Life Technologies). RNA integrity was assessed on the Agilent 2100 Bioanalyzer instrument and only RNA samples with integrity scores > 9.5 (median 10.0) were used. Stranded libraries for paired-end sequencing were prepared as described in Parkhomchuk, D., Borodina, T., Amstislavskiy, V., Banaru, M., Hallen, L., Krobitsch, S., Lehrach, H., and Soldatov, A. (2009). Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37, e123.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: AB2.2 ESC nuclear polyA


E-MTAB-3198:ESC Control ASO Rep4

text: CSHL

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3198:ESC Control ASO Rep4

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep4


E-MTAB-3198:ESC PAT14 ASO#2 Rep3

text: CSHL

text: public

characteristic: SRA accession

text: E-MTAB-3198:ESC PAT14 ASO#2 Rep3

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#2 Rep3


E-MTAB-3198:ESC Firre ASO#2 Rep3

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#2 Rep3

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: whole cell

text: house mouse

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#2 Rep3


E-MTAB-3198:ESC PAT14 ASO#2 Rep2

text: CSHL

text: public

text: E-MTAB-3198:ESC PAT14 ASO#2 Rep2

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#2 Rep2

characteristic: title


E-MTAB-3198:ESC Firre ASO#5 Rep4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#5 Rep4

text: ArrayExpress

text: embryonic stem cell

text: whole cell

characteristic: cellular component

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#5 Rep4


E-MTAB-3198:AB2.2 NPC nuclear polyA

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSHL

text: public

text: E-MTAB-3198:AB2.2 NPC nuclear polyA

text: ArrayExpress

text: neural progenitor cell

text: nucleus

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: AB2.2 NPC nuclear polyA


E-MTAB-3198:ESC Firre ASO#2 Rep4

text: CSHL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-3198:ESC Firre ASO#2 Rep4

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#2 Rep4


E-MTAB-3198:ESC PAT14 ASO#3 Rep2

text: CSHL

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3198:ESC PAT14 ASO#3 Rep2

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#3 Rep2


E-MTAB-3198:ESC Control ASO Rep2

text: CSHL

text: public

text: E-MTAB-3198:ESC Control ASO Rep2

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: whole cell

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep2


E-MTAB-3198:ESC PAT14 ASO#3 Rep1

text: CSHL

text: public

text: E-MTAB-3198:ESC PAT14 ASO#3 Rep1

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: whole cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#3 Rep1


E-MTAB-3198:ESC Firre ASO#2 Rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#2 Rep1

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#2 Rep1

characteristic: title


E-MTAB-3198:ESC Control ASO Rep3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSHL

text: public

text: E-MTAB-3198:ESC Control ASO Rep3

text: ArrayExpress

text: embryonic stem cell

text: whole cell

characteristic: cellular component

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep3


E-MTAB-3198:ESC Control ASO Rep7

text: CSHL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-3198:ESC Control ASO Rep7

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: ESC Control ASO Rep7


E-MTAB-3198:ESC Control ASO Rep8

text: CSHL

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3198:ESC Control ASO Rep8

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep8


E-MTAB-3198:ESC Firre ASO#5 Rep1

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#5 Rep1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: whole cell

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#5 Rep1


E-MTAB-3198:ESC Firre ASO#5 Rep3

text: CSHL

text: public

text: E-MTAB-3198:ESC Firre ASO#5 Rep3

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: whole cell

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Firre ASO#5 Rep3


E-MTAB-3198:ESC PAT14 ASO#3 Rep3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CSHL

text: public

text: E-MTAB-3198:ESC PAT14 ASO#3 Rep3

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: male

text: ESC PAT14 ASO#3 Rep3


E-MTAB-3198:ESC Control ASO Rep1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CSHL

text: public

text: E-MTAB-3198:ESC Control ASO Rep1

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep1

characteristic: title


E-MTAB-3198:ESC Control ASO Rep6

text: CSHL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-3198:ESC Control ASO Rep6

text: ArrayExpress

text: embryonic stem cell

text: whole cell

characteristic: cellular component

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: ESC Control ASO Rep6


E-MTAB-3198:ESC Firre ASO#2 Rep2

text: CSHL

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3198:ESC Firre ASO#2 Rep2

text: ArrayExpress

text: embryonic stem cell

text: whole cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: ESC Firre ASO#2 Rep2


text: Human

characteristic: ArrayExpress-Species

text: PhiX DNA spike-in at 50%

characteristic: ArrayExpress-Treatment

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: DNA library from AML cell lines after CRISPR/CAS9 screens

characteristic: sample description

text: Homo sapiens


text: 180 days

characteristic: ArrayExpress-Age

text: Gonadal adipose tissue

characteristic: ArrayExpress-OrganismPart

text: Lean

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: DNA extracted from gonadal adipose tissue

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TGAC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: TGAC

characteristic: broker name

text: chimpanzee

text: CA_x062 wk4

characteristic: description

text: Pan troglodytes

characteristic: organism

text: Pan troglodytes

characteristic: title


text: TGAC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: TGAC

text: chimpanzee

text: CF_x176 wk52

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CH_x284 wk4

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CI_x284 wk48

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CJ_ni wk2

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CB_x062 wk52

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CC_x310 wk4

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CD_x310 wk52

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CE_x176 wk2

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C4_Leon 2001

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C3_Soc 2001

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C2_No 2001

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C1_Ni 2001

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CZ_Ni wk0

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CY_x115 wk52

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CX_x115 wk2

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: CW_x062 wk2

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C8_x115 wk4

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C6_Maya 2001

text: Pan troglodytes

text: Pan troglodytes


text: TGAC

text: public

text: TGAC

text: chimpanzee

text: C5_Bu 2001

text: Pan troglodytes

text: Pan troglodytes


text: 180 days

characteristic: ArrayExpress-Age

text: Tail

characteristic: ArrayExpress-OrganismPart

text: Lean

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: DNA extracted from tail

characteristic: sample description

text: Mus musculus


text: 180 days

text: Gonadal adipose tissue

text: Obese

text: male

text: Mus Musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

characteristic: strain


text: 180 days

text: Tail

text: Obese

text: male

text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

characteristic: organism

text: DNA extracted from tail

text: Mus musculus


E-MTAB-3087:Sample 2

text: public

characteristic: SRA accession

text: E-MTAB-3087:Sample 2

tag: Namespace:University of science of technology of China

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: normal tissue adjacent to tumour

characteristic: clinical information

text: human

text: Protocols: Total RNA was extracted from ten pairs of fresh-frozen penile cancer and matched histologically normal penile tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. RNA with good quality e.g. A260/A280>1.8 was used for further experiments. Meanwhile, the RNA integrity was evaluated by with DNA chip facilitated by Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA). Two smRNA libraries were constructed using homogenized and pooled total RNAs of ten individuals for each group (normal and cancerous penile tissues). For each group, 10 microgram of total RNA was used for library construction with a Small RNA Sample Prep Kit (Illumina, USA) following the manufacturer’s instructions with minor modifications. Briefly, after 15% (w/v) denaturing PAGE (polyacrylamide gel electrophoresis), the 18- to 28-nt fraction of total RNA was excised, purified, and ligated to 3’ and 5’ RNA adaptors using T4 RNA ligase. The adaptor-ligated sRNAs were introduced to RT-PCR with 15 cycles of PCR amplification. The PCR products (approximately 90-bp, corresponding to sRNA + adaptors) were purified on 4% agarose gels to create the libraries.

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-3087:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of science of technology of China

text: public

text: E-MTAB-3087:Sample 1

text: ArrayExpress

text: human

text: penile cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 1


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Charles University in Prague, Faculty of Science, Department of Cell Biology

text: ArrayExpress

text: fission yeast

text: Protocols: Five hundred millilitre cultures of an untagged strain and strains expressing TAP-tagged CSL proteins were grown in the rich YES medium (0.5% yeast extract, 3% glucose, SP supplements (Formedium)) at 32 degrees C to OD 1.0, fixed with 1% formaldehyde for 30 min, quenched with 125 mM glycine, washed with PBS and broken with glass beads in Lysis Buffer (50 mM Hepes pH 7.6, 1 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% sodium deoxycholate, FY protease inhibitors (Serva)) using the FastPrep24 machine (MP Biomedicals). A. Chromatin Immunoprecipitation protocol A.2 Preparing extract and setting up immunoprecipitations A.2.1 Thaw cross-linked cell pellet on ice for 5 min. Resuspend pellets (you have 2 eppendorf tubes per sample) in total of 800 microlitres ice-cold Lysis buffer (add 750 microlitres of LB) containing 2x protease inhibitors (from Roche EDTA-free), 1 mM PMSF. A.2.3 Prepare a 4L bucket filled with ice and ice-cold dH2O. You will need it for sonication. A.2.3 Pour 500-600 microlitres of acid-washed glass beads and break in FastPrep 9-12x20sec at 5.5 with 5min incubations on ice in between (DO the breaking in 2 ml screw-cap tubes). Visualize a small aliquot in the microscope of cells to estimate the breaking efficiency (should be >90%). A.2.4 Poke a hole in the bottom of the tube with broken cells with a sterile needle after flaming it and place in a clean tube. Put the 2 tubes in a 50ml falcon and spin for 1min at 1000 rpm at 4ºC to collect the lysate. Wash the glass beads again with 400 microlitres lysis buffer (with inhibitors), spin and pool with the first flow-through (the additional washing of beads increases yield). A.2.5 Spin the lysate for 10min at 20000rcf, pour off supernatant and wash once more with 800 microlitres lysis buffer (with inhibitors) (the additional wash increases yield). A.2.6 After the wash, resuspend the pellet in total 750 microlitres of cold Lysis buffer and pool both eppendorf tubes into one 2 ml tube (you’re back to 1 Eppendorf tube / sample). Re-add PMSF to 1 mM. Split into 5x 300 microlitre aliquots in Axygen 1.5 ml tubes (this is the volume and tubes for which sonication was calibrated). A.2.7 Sonicate 6 x 5min with 30sec ON/ 30sec OFF at HIGH setting, refill the water bath with cold water (no crushed ice chips!!) after each 5min interval (Bioruptor settings). This procedure will produce fragments chromatin fragments of ~200bps. The conditions of sonication have typically to be set-up in each lab. The volume and concentration of the extract as well as the duration and strength of the sonication will affect the size of the sheared chromatin. A.2.8 Spin the sonicated material at 20000rcf for 10min at 4 degrees C and collect the supernatant (chromatin extract, CE). Store the CE at -70 degrees C. Use 5 microlitres CE to check protein concentration / Bradford (should get > 10 mg/ml) Use 50 microlitres CE to check sonication efficiency (in screw-cap tubes): Add 150 microlitres TES, incubate 65 degrees C, O/N. Add 200 microlitres TE, 5 microlitres of DNase-free RNase (0.5 mg/ml), incubate 37 degrees C, 60min. Add 7 microlitres proteinase K (20 mg/ml), incubate 55 degrees C, 2 hours. Add 400 microlitres phenol/chloroform/IAA (25:24:1), vortex 20 sec, spin full speed, 10 min, 4 degrees C. Collect the upper water phase (~400 microlitres) in a 1.5 ml screwcap tube. Re-add 100 microlitres TE to the lower organic phase, vortex 20 sec, spin full speed, 10 min, 4 degrees C (this increases yield). Collect the upper water phase and pool with the first one (~500 microlitres in total). Add 500 microlitres chloroform/IAA, vortex 5 sec, spin full speed, 5 min, 4 degrees C. Collect the upper water phase. Add 1 microlitre glycogen 20 mg/ml, 50 microlitres NaAc 3M, 1.25 ml ice-cold EtOH 100%. Incubate -70 degrees C, 30 min. Spin full speed, 15 min, 4 degrees C. Wash with 1ml ice-cold EtOH 70% (caution: loose pellet), spin 5 min 4 degrees C. Dry pellet in speedvac and resuspend in 10 microlitres water. Analyze 1 microlitre on Bioanalyzer or load everything on agarose 1%, 80V, EtBr stain. These samples can be used as 'input' for library construction. A.3 Immunopreciptitations, crosslink reversal and DNA purification. A.3.1 Keep 50 microlitres of CE for input (it can be stored at -20C). A.3.2 Prepare magnetic beads (all incubations and washes at 4 degrees C): Add 100 microlitres magnetic beads (Protein G-coated for rabbit/goat polyclonal Ab or mouse/sheep IgG-coated for TAP-tagged proteins, Invitrogen, Dynabeads) to a 1.5 ml microfuge tube. Add 1 ml block solution (0.5% BSA (w/v) in LB). Set up 1 tube per IP. Collect the beads using magnetic stand. Remove supernatant by aspiration. Wash beads in 1.0 ml block solution two more times. Resuspend beads in block solution and add 5 micrograms of antibody in a final volume of 250 microlitres. Incubate overnight or a minimum of 4 h on a rotating platform at 4 degrees C. Wash magnetic beads as described above (3 times in 1 ml block solution). Resuspend in 50 microlitres block solution. A.3.3 Use 5 mg CE (from Bradford) per sample, per IP. Bring up to 500 microlitres with LB (without inhibitors). A.3.4 Add the 50 microlitres antibody/magnetic bead mix to cell lysates. Gently mix at 4 degrees C in rotating wheel overnight. A.3.5 Collect the beads using magnetic stand. Remove supernatant by aspiration. Wash beads in 0.8ml of each buffer in semi-cold conditions: 2x in Lysis buffer 2x in Lysis 500 2x in LiCl/NP-40 1x in TE. A.3.6 Resuspend the beads in 200 microlitres elution buffer. Elute and perform reverse crosslinking at 65 degrees C for 6-18 h. Resuspend beads in the first 15 min with brief vortexing every 5 min. A.3.7 For the input, add 150 microlitres (3x the volume) of TES, also perform reverse crosslinking at 65 degrees C for 6-18 h and treat subsequently as the samples. The volume is increased to dilute out the SDS. A.3.8 Digest proteins and RNA: Add 200 microlitres TE, 5 microlitres of DNase-free RNase (0.5 mg/ml), incubate 37 degrees C, 60min Add 7 microlitre proteinase K (20 mg/ml), incubate 55 degrees C, 2 hours. The RNA is removed because it may interfere with the labelling reaction. A.3.9 Purify DNA (in screw-cap tubes): Add 400 microlitres phenol/chloroform/IAA (25:24:1), vortex 20 sec, spin full speed, 10 min, 4 degrees C. Collect the upper water phase (~400 microlitres) in a 1.5 ml screwcap tube. Re-add 100 microlitres TE to the lower organic phase, vortex 20 sec, spin full speed, 10 min, 4 degrees C (this increases yield). Collect the upper water phase and pool with the first one (~500 microlitres in total) Add 500 microlitres chloroform/IAA, vortex 5 sec, spin full speed, 5 min, 4 degrees C. Collect the upper water phase to a 2 ml tube. Add 1 microlitre glycogen 20 mg/ml, 50 microlitres NaAc 3M, 1.25 ml ice-cold EtOH 100% Incubate -70 degrees C, 1-2 h. Spin full speed, 15 min, 4 degrees C, process only 4 samples at a time for the next steps. Remove EtOH (caution: loose and tiny tiny pellet) and wash with 1ml ice-cold EtOH 70% (caution: loose and tiny tiny pellet), spin 5 min 4 degrees C. Dry pellet in speedvac. Resuspend in 25 microlitres TE, incubate at RT for 10 min. Purify on a Qiagen column to get rid of low-molecular weight debris and run 1 microlitre on a Bioanalyzer. Store -20 degrees C. These DNA samples can be now l used as starting material for ChIP-seq library preparation! Buffers: Lysis Buffer: 50mM Hepes pH 7.6 1mM EDTA pH8 150mM NaCl 1% Triton X-100 0.1% Na-Doc Protease Inhibitors; Lysis 500 Buffer: 50mM Hepes pH 7.6 1mM EDTA pH8 500mM NaCl 1% Triton X-100 0.1% Na-Doc; LiCl/NP-40 Buffer: 10mM Tris-HCl pH8 1mM EDTA pH8 250mM LiCl 1% NP-40 1% Na-Doc; Elution Buffer: 50mM Tris-HCL pH8 10mM EDTA 1% SDS; TE Buffer: 10mM Tris-HCL pH8 1mM EDTA; TES Buffer: 10mM Tris-HCL pH8 1mM EDTA 1% SDS Perform end-repair of the DNA use the End-it kit (Epicentre Biotechnologies, ER0720) 1. Mix together (total reaction volume 50 microlitres): 150 ng DNA in 34 microlitres total (see Nanodrop), 5 microlitres End-It 10X buffer, 5 microlitres ATP (10 mM), 5 microlitres dNTPs mix (2.5 mM), 1 microlitre End-It enzyme mix. 2. Incubate for 1 hour at RT. 3. Clean up samples using the DNA Clean&Concentrator-5 kit (Zymo Research), elute with 3x 11 microlitres water preheated to 50 degrees C. 4. Chill on ice. Add “A” bases to the DNA 1. Mix together (total reaction volume 50 microlitres): 32 microlitres DNA eluate, 5 microlitres Klenow 10X buffer (NEB, B7002S), 10 microlitres dATP (1 mM), 3 microlitres Klenow 3'-5' exo- (NEB, M0212L). 2. Incubate for 30 min at 37 degrees C. 3. Clean up samples using the DNA Clean&Concentrator-5 kit (Zymo Research), elute with 9 microlitres water preheated to 50 degrees C (incubate columns 1 min at RT before spinning). 4. Chill on ice. Ligate sequencing adapters to DNA fragments. Use adapters from the Illumina Multiplexed PE Sample Preparation Kit (1005361). 1. Mix together (total reaction volume 25 microlitres): 8 microlitres DNA eluate, 12.5 microlitres Quick Ligation Reaction Buffer (NEB, M2200L), 2 microlitres of 40x diluted Index PE Adapter Oligo Mix (Illumina), 2.5 microlitres Quick T4 DNA Ligase (NEB, M2200L). 2. Incubate for 15 min at RT. 3. Clean up samples using the DNA Clean&Concentrator-5 kit (Zymo Research), elute with 3x 15 microlitres water preheated to 50 degrees C. 4. Chill on ice. 5. Use 22 microlitres of the eluate for PCR and keep the other 22 microlitres as backup. Amplify adapter-modified DNA by PCR. Use primers from the Illumina Multiplexed PE Sample Preparation Kit (1005361). 1. Mix together in PCR tubes (total reaction volume 50 microlitres): 22 microlitres DNA eluate, 25 microlitres Phusion Master Mix with HF Buffer (NEB, F-531L), 1 microlitre PCR Primer InPE 1.0 (Illumina), 1 microlitre PCR Primer InPE 2.0 (Illumina), 1 microlitre PCR Primer Index<2/4/5/6/7/12> (Illumina). 2. Run PCR program: 98 degrees C 30 sec, 98 degrees C 10 sec, 65 degrees C 30 sec, 72 degrees C 30 sec, goto step 2, 17 times, 72 degrees C 5 min, 4 degrees C hold. 3. Clean up samples using the AMPure XP beads (Agencourt) - two successive rounds of purification are employed to get rid of DNA <100 bp and >300 bp. Place the beads at RT for 30 min before using them (do not use QiaQuick columns as they do not remove primers and adaptor dimers efficiently): “negative beads” 1. Mix AMPure XP beads with the PCR reaction in a 0.8 (beads) to 1.0 ratio (DNA) in 1.5 ml tubes. Under such conditions the beads selectively bind only higher molecular weight (>300 bp) fragments. 2. Incubate 5 min at RT. 3. Place tubes on a magnetic stand for 2 min. 4. Collect flow-through (this contains your DNA!!!). “positive beads” 1. Mix AMPure XP beads with the flow-through from the previous step in a 1.8 (beads) to 1.0 ratio (DNA) in 1.5 ml tubes. Only fragments >100 bp are bound (removes primers and also minimizes the adapter dimer content!). 2. Incubate 5 min at RT. 3. Place tubes on a magnetic stand for 2 min. 4. Keep the tubes on the stand. Remove flow-through. 5. While still on the stand, wash the beads with 70% EtOH (gently tilt the stand to allow the EtOH to reach the beads) for 30 sec. 6. Remove the EtOH and repeat the wash once again. 7. Open the tubes and let the beads air-dry for <5 min at RT (do not overdry - manifests as cracks in the bead mass). 8. Remove the tubes from the stand and elute with 40 microlitres water (mix by pipetting) for 1 min (do not use pre-heated water for elution as this might denature the library, which needs to remain double-stranded for sequencing). 9. Place tubes on a magnetic stand for 2 min. 10. Collect the eluate in a new tube and keep on ice. Check library quality on Bioanalyzer. Use DNA 1000 chips for high-concentration samples (inputs) and DNA High sensitivity chips for low-concentration samples (IPs).

text: h+s

characteristic: genotype

text: exponential growth, unsynchronized

characteristic: growth condition

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: Charles University in Prague, Faculty of Science, Department of Cell Biology

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fission yeast

characteristic: common name

text: Protocols: Five hundred millilitre cultures of an untagged strain and strains expressing TAP-tagged CSL proteins were grown in the rich YES medium (0.5% yeast extract, 3% glucose, SP supplements (Formedium)) at 25 degrees C to OD 0.8. All strains contained a temperature-sensitive allele cdc25-22. Cell were arrested at late G2 phase by incubation at 36.5 degrees C for 4 hours, and then released synchronously into cell cycle by rapid cooling of cultures to 25 degrees C. Cell cycle progression was monitored by microscopy (division septum formation) and collected in S phase (coincides with peak of septation). Cells were fixed with 1% formaldehyde for 30 min, quenched with 125 mM glycine, washed with PBS and broken with glass beads in Lysis Buffer (50 mM Hepes pH 7.6, 1 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% sodium deoxycholate, FY protease inhibitors (Serva)) using the FastPrep24 machine (MP Biomedicals) A. Chromatin Immunoprecipitation protocol A.2 Preparing extract and setting up immunoprecipitations A.2.1 Thaw cross-linked cell pellet on ice for 5 min. Resuspend pellets (you have 2 eppendorf tubes per sample) in total of 800 microlitres ice-cold Lysis buffer (add 750 microlitres of LB) containing 2x protease inhibitors (from Roche EDTA-free), 1 mM PMSF. A.2.3 Prepare a 4L bucket filled with ice and ice-cold dH2O. You will need it for sonication. A.2.3 Pour 500-600 microlitres of acid-washed glass beads and break in FastPrep 9-12x20sec at 5.5 with 5min incubations on ice in between (DO the breaking in 2 ml screw-cap tubes). Visualize a small aliquot in the microscope of cells to estimate the breaking efficiency (should be >90%). A.2.4 Poke a hole in the bottom of the tube with broken cells with a sterile needle after flaming it and place in a clean tube. Put the 2 tubes in a 50ml falcon and spin for 1min at 1000 rpm at 4ºC to collect the lysate. Wash the glass beads again with 400 microlitres lysis buffer (with inhibitors), spin and pool with the first flow-through (the additional washing of beads increases yield). A.2.5 Spin the lysate for 10min at 20000rcf, pour off supernatant and wash once more with 800 microlitres lysis buffer (with inhibitors) (the additional wash increases yield). A.2.6 After the wash, resuspend the pellet in total 750 microlitres of cold Lysis buffer and pool both eppendorf tubes into one 2 ml tube (you’re back to 1 Eppendorf tube / sample). Re-add PMSF to 1 mM. Split into 5x 300 microlitre aliquots in Axygen 1.5 ml tubes (this is the volume and tubes for which sonication was calibrated). A.2.7 Sonicate 6 x 5min with 30sec ON/ 30sec OFF at HIGH setting, refill the water bath with cold water (no crushed ice chips!!) after each 5min interval (Bioruptor settings). This procedure will produce fragments chromatin fragments of ~200bps. The conditions of sonication have typically to be set-up in each lab. The volume and concentration of the extract as well as the duration and strength of the sonication will affect the size of the sheared chromatin. A.2.8 Spin the sonicated material at 20000rcf for 10min at 4 degrees C and collect the supernatant (chromatin extract, CE). Store the CE at -70 degrees C. Use 5 microlitres CE to check protein concentration / Bradford (should get > 10 mg/ml) Use 50 microlitres CE to check sonication efficiency (in screw-cap tubes): Add 150 microlitres TES, incubate 65 degrees C, O/N. Add 200 microlitres TE, 5 microlitres of DNase-free RNase (0.5 mg/ml), incubate 37 degrees C, 60min. Add 7 microlitres proteinase K (20 mg/ml), incubate 55 degrees C, 2 hours. Add 400 microlitres phenol/chloroform/IAA (25:24:1), vortex 20 sec, spin full speed, 10 min, 4 degrees C. Collect the upper water phase (~400 microlitres) in a 1.5 ml screwcap tube. Re-add 100 microlitres TE to the lower organic phase, vortex 20 sec, spin full speed, 10 min, 4 degrees C (this increases yield). Collect the upper water phase and pool with the first one (~500 microlitres in total). Add 500 microlitres chloroform/IAA, vortex 5 sec, spin full speed, 5 min, 4 degrees C. Collect the upper water phase. Add 1 microlitre glycogen 20 mg/ml, 50 microlitres NaAc 3M, 1.25 ml ice-cold EtOH 100%. Incubate -70 degrees C, 30 min. Spin full speed, 15 min, 4 degrees C. Wash with 1ml ice-cold EtOH 70% (caution: loose pellet), spin 5 min 4 degrees C. Dry pellet in speedvac and resuspend in 10 microlitres water. Analyze 1 microlitre on Bioanalyzer or load everything on agarose 1%, 80V, EtBr stain. These samples can be used as 'input' for library construction. A.3 Immunopreciptitations, crosslink reversal and DNA purification. A.3.1 Keep 50 microlitres of CE for input (it can be stored at -20C). A.3.2 Prepare magnetic beads (all incubations and washes at 4 degrees C): Add 100 microlitres magnetic beads (Protein G-coated for rabbit/goat polyclonal Ab or mouse/sheep IgG-coated for TAP-tagged proteins, Invitrogen, Dynabeads) to a 1.5 ml microfuge tube. Add 1 ml block solution (0.5% BSA (w/v) in LB). Set up 1 tube per IP. Collect the beads using magnetic stand. Remove supernatant by aspiration. Wash beads in 1.0 ml block solution two more times. Resuspend beads in block solution and add 5 micrograms of antibody in a final volume of 250 microlitres. Incubate overnight or a minimum of 4 h on a rotating platform at 4 degrees C. Wash magnetic beads as described above (3 times in 1 ml block solution). Resuspend in 50 microlitres block solution. A.3.3 Use 5 mg CE (from Bradford) per sample, per IP. Bring up to 500 microlitres with LB (without inhibitors). A.3.4 Add the 50 microlitres antibody/magnetic bead mix to cell lysates. Gently mix at 4 degrees C in rotating wheel overnight. A.3.5 Collect the beads using magnetic stand. Remove supernatant by aspiration. Wash beads in 0.8ml of each buffer in semi-cold conditions: 2x in Lysis buffer 2x in Lysis 500 2x in LiCl/NP-40 1x in TE. A.3.6 Resuspend the beads in 200 microlitres elution buffer. Elute and perform reverse crosslinking at 65 degrees C for 6-18 h. Resuspend beads in the first 15 min with brief vortexing every 5 min. A.3.7 For the input, add 150 microlitres (3x the volume) of TES, also perform reverse crosslinking at 65 degrees C for 6-18 h and treat subsequently as the samples. The volume is increased to dilute out the SDS. A.3.8 Digest proteins and RNA: Add 200 microlitres TE, 5 microlitres of DNase-free RNase (0.5 mg/ml), incubate 37 degrees C, 60min Add 7 microlitre proteinase K (20 mg/ml), incubate 55 degrees C, 2 hours. The RNA is removed because it may interfere with the labelling reaction. A.3.9 Purify DNA (in screw-cap tubes): Add 400 microlitres phenol/chloroform/IAA (25:24:1), vortex 20 sec, spin full speed, 10 min, 4 degrees C. Collect the upper water phase (~400 microlitres) in a 1.5 ml screwcap tube. Re-add 100 microlitres TE to the lower organic phase, vortex 20 sec, spin full speed, 10 min, 4 degrees C (this increases yield). Collect the upper water phase and pool with the first one (~500 microlitres in total) Add 500 microlitres chloroform/IAA, vortex 5 sec, spin full speed, 5 min, 4 degrees C. Collect the upper water phase to a 2 ml tube. Add 1 microlitre glycogen 20 mg/ml, 50 microlitres NaAc 3M, 1.25 ml ice-cold EtOH 100% Incubate -70 degrees C, 1-2 h. Spin full speed, 15 min, 4 degrees C, process only 4 samples at a time for the next steps. Remove EtOH (caution: loose and tiny tiny pellet) and wash with 1ml ice-cold EtOH 70% (caution: loose and tiny tiny pellet), spin 5 min 4 degrees C. Dry pellet in speedvac. Resuspend in 25 microlitres TE, incubate at RT for 10 min. Purify on a Qiagen column to get rid of low-molecular weight debris and run 1 microlitre on a Bioanalyzer. Store -20 degrees C. These DNA samples can be now l used as starting material for ChIP-seq library preparation! Buffers: Lysis Buffer: 50mM Hepes pH 7.6 1mM EDTA pH8 150mM NaCl 1% Triton X-100 0.1% Na-Doc Protease Inhibitors; Lysis 500 Buffer: 50mM Hepes pH 7.6 1mM EDTA pH8 500mM NaCl 1% Triton X-100 0.1% Na-Doc; LiCl/NP-40 Buffer: 10mM Tris-HCl pH8 1mM EDTA pH8 250mM LiCl 1% NP-40 1% Na-Doc; Elution Buffer: 50mM Tris-HCL pH8 10mM EDTA 1% SDS; TE Buffer: 10mM Tris-HCL pH8 1mM EDTA; TES Buffer: 10mM Tris-HCL pH8 1mM EDTA 1% SDS Sequencing libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (E6240L) + NEB Multiplex Oligos for Illumina (E7335). End-repair, dA-tailing, Adaptor ligation, Size selection were all performed according to the manufacturer's instructions. PCR Enrichment of Adaptor Ligated DNA was done in 18 cycles with NEBNext High-Fidelity 2X PCR Master Mix. All clean-ups and size selection performed on AMPure XP beads. Library quality and quantity assessed was done on Bioanalyzer (High Sensitivity DNA chip; no sample dilution).

text: cdc25-22 h-

text: synchronized in S-phase

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

characteristic: strain


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: fission yeast

text: cdc25-22 cbf11-ctap4::natR h+

text: synchronized in S-phase

text: Schizosaccharomyces pombe

characteristic: organism

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fission yeast

characteristic: description

text: h+s

text: exponential growth, unsynchronized

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: fission yeast

text: cbf11-ctap4::natR h+

text: exponential growth, unsynchronized

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: fission yeast

text: cbf12-ctap4::natR h+

text: exponential growth, unsynchronized

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


characteristic: External Id

text: public

text: ArrayExpress

text: fission yeast

text: cdc25-22 cbf12-ctap4::natR h+

characteristic: genotype

text: synchronized in S-phase

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe


text: Max-Delbruck-Centrum Berlin

characteristic: INSDC center name

text: public

tag: Namespace:Max-Delbruck-Centrum Berlin

text: ArrayExpress

text: macrophage

characteristic: cell type

text: house mouse

text: Protocols: Primary bone-marrow-derived murine macrophages were grown in RPMI1640 (GIBCO) supplemented with 10% FCS, 10mM HEPES, 1% Penicillin-Streptomycin, 5uM beta-mercaptoethanol at a density of 40 million cells per 150cm(2) cell culture flask. After attachment for 2h cells were stimulated with 10ng/ml LPS (Sigma-Aldrich) [M(LPS)] or 10ng/ml IL-4 (R&D Systems) and 10ng/ml IL-13 (Invitrogen) for 24h. 40mM NaCl were added where applicable. 40 million macrophages per group were used to isolate nuclei by centrifugation through a dense sucrose cushion. Subsequently, samples were digested with micrococcal nuclease (Sigma-Aldrich) to generate native chromatin templates consisting mainly of mononucleosomes. Abundance of mononucleosomal DNA was confirmed by gel electrophoresis. Chromatin was extracted with RIPA buffer and cellular debris removed by centrifugation. Subsequently, chromatin was precipitated over night at 4C with anti-H3K4me3 (NEB #9751 S) or anti-H4Ac (Millipore #06-866) bound to Dynabeads Protein A (Invitrogen #10002D). MinElute PCR Purification Kit (QIAGEN) was used to purify immunoprecipitated DNA after Proteinase K (Sigma-Aldrich) digestion at 65C for 4h. 50ng of purified DNA was used for library preparation with the TruSeq ChIP sample preparation kit (Illumina) according to the manufacturer's instructions.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: 10ng/ml IL-4, 10ng/ml IL-13

characteristic: stimulus


text: Max-Delbruck-Centrum Berlin

characteristic: INSDC first public

text: public

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: Mus musculus

characteristic: strain


text: Mouse

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

characteristic: ArrayExpress-Sex

text: Mus castaneous

text: CASTEiJ

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: CASTEiJ


text: female

text: Mus spretus

text: SpretEiJ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: western wild mouse

text: Mus spretus

text: Mus spretus

text: SpretEiJ


text: Danio rerio

text: TL/AB

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

text: Exome

characteristic: sample description

text: Danio rerio

text: TL/AB


M.G

text: Universite de Poitiers

characteristic: INSDC first public

text: public

text: M.G

tag: Namespace:Universite de Poitiers

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

characteristic: host

text: Laboratory

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

characteristic: tissue type

text: M.G

characteristic: title


M.N

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: M.N

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: M.N


M.C

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: M.C

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Caeca

text: M.C


M.T

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: M.T

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: M.T


M.F

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: M.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: M.F


FNI.H

text: Universite de Poitiers

text: public

text: FNI.H

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: FNI.H


FNI.G

text: Universite de Poitiers

text: public

text: FNI.G

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Laboratory

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FNI.G


FNI.N

text: Universite de Poitiers

text: public

text: FNI.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

characteristic: tissue type

text: FNI.N

characteristic: title


FNI.C

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FNI.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: FNI.C


FNI.T

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: FNI.T

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FNI.T


FNI.F

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: FNI.F

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: FNI.F


FC.H

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: FC.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Haemolymph

text: FC.H


FC.G

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: FC.G

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FC.G


FC.N

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: FC.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: FC.N


FC.C

text: Universite de Poitiers

text: public

text: FC.C

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: FC.C


FC.T

text: Universite de Poitiers

text: public

text: FC.T

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Laboratory

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FC.T


FC.F

text: Universite de Poitiers

text: public

text: FC.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

characteristic: tissue type

text: FC.F

characteristic: title


FM.H

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FM.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: FM.H


FM.G

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: FM.G

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FM.G


FM.N

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: FM.N

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: FM.N


FM.C

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: FM.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Caeca

text: FM.C


FM.T

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: FM.T

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FM.T


FM.F

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: FM.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: FM.F


FP.H

text: Universite de Poitiers

text: public

text: FP.H

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: FP.H


FP.G

text: Universite de Poitiers

text: public

text: FP.G

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Laboratory

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FP.G


FP.N

text: Universite de Poitiers

text: public

text: FP.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

characteristic: tissue type

text: FP.N

characteristic: title


FP.C

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FP.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: FP.C


FP.T

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: FP.T

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FP.T


FP.F

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: FP.F

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Laboratory

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: FP.F


Mav.H

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: Mav.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Haemolymph

text: Mav.H


Mav.G

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: Mav.G

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Mav.G


Mav.N

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Mav.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: Mav.N


Mav.C

text: Universite de Poitiers

text: public

text: Mav.C

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: Mav.C


Mav.T

text: Universite de Poitiers

text: public

text: Mav.T

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Availles-Thouarsais

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Mav.T


Mav.F

text: Universite de Poitiers

text: public

text: Mav.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

characteristic: tissue type

text: Mav.F

characteristic: title


FCav.H

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FCav.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: FCav.H


FCav.G

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: FCav.G

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FCav.G


FCav.N

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: FCav.N

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: FCav.N


FCav.C

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: FCav.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Caeca

text: FCav.C


FCav.T

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: FCav.T

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FCav.T


FCav.F

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: FCav.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: FCav.F


FMav.H

text: Universite de Poitiers

text: public

text: FMav.H

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: FMav.H


FMav.G

text: Universite de Poitiers

text: public

text: FMav.G

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Availles-Thouarsais

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: FMav.G


FMav.N

text: Universite de Poitiers

text: public

text: FMav.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

characteristic: tissue type

text: FMav.N

characteristic: title


FMav.C

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FMav.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: FMav.C


FMav.T

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: FMav.T

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: FMav.T


FMav.F

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: FMav.F

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: FMav.F


Mpm.H

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: Mpm.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Haemolymph

text: Mpm.H


Mpm.G

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: Mpm.G

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Mpm.G


Mpm.N

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Mpm.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: Mpm.N


Mpm.C

text: Universite de Poitiers

text: public

text: Mpm.C

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: Mpm.C


Mpm.T

text: Universite de Poitiers

text: public

text: Mpm.T

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Plaine Mothaise

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Mpm.T


Mpm.F

text: Universite de Poitiers

text: public

text: Mpm.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

characteristic: tissue type

text: Mpm.F

characteristic: title


Fpm.H

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Fpm.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: Fpm.H


Fpm.G

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: Fpm.G

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Fpm.G


Fpm.N

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: Fpm.N

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: Fpm.N


Fpm.C

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: Fpm.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Caeca

text: Fpm.C


Fpm.T

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: Fpm.T

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Fpm.T


Fpm.F

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Fpm.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: Fpm.F


Mthu.H

text: Universite de Poitiers

text: public

text: Mthu.H

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: Mthu.H


Mthu.G

text: Universite de Poitiers

text: public

text: Mthu.G

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Thure

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Mthu.G


Mthu.N

text: Universite de Poitiers

text: public

text: Mthu.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

characteristic: tissue type

text: Mthu.N

characteristic: title


Mthu.C

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Mthu.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: Mthu.C


Mthu.T

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: Mthu.T

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Mthu.T


Mthu.F

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: Mthu.F

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: Mthu.F


Fthu.H

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: Fthu.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Haemolymph

text: Fthu.H


Fthu.G

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: Fthu.G

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Fthu.G


Fthu.N

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Fthu.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: Fthu.N


Fthu.C

text: Universite de Poitiers

text: public

text: Fthu.C

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: Fthu.C


Fthu.T

text: Universite de Poitiers

text: public

text: Fthu.T

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Thure

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Fthu.T


Fthu.F

text: Universite de Poitiers

text: public

text: Fthu.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Thure

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

characteristic: tissue type

text: Fthu.F

characteristic: title


Frick.H

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Frick.H

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: Frick.H


Frick.G

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: Frick.G

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Frick.G


Frick.N

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: Frick.N

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

text: Frick.N


Frick.C

characteristic: ENA-FIRST-PUBLIC

text: Universite de Poitiers

text: public

text: Frick.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

characteristic: organism

text: Armadillidium vulgare

text: Caeca

text: Frick.C


Frick.T

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite de Poitiers

text: public

text: Frick.T

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Frick.T


Frick.F

text: Universite de Poitiers

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Frick.F

text: common pillbug

text: France

text: Armadillidium vulgare

text: Availles-Thouarsais

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: Frick.F


Mis.H

text: Universite de Poitiers

text: public

text: Mis.H

text: common pillbug

characteristic: description

text: France

characteristic: geographic location (country and/or sea)

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Haemolymph

text: Mis.H


Mis.G

text: Universite de Poitiers

text: public

text: Mis.G

text: common pillbug

text: France

text: Armadillidium vulgare

characteristic: host

text: Plaine Mothaise

characteristic: isolation source

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Gonads

text: Mis.G


Mis.N

text: Universite de Poitiers

text: public

text: Mis.N

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Nerve Cord

characteristic: tissue type

text: Mis.N

characteristic: title


Mis.C

text: Universite de Poitiers

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Mis.C

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Caeca

text: Mis.C


Mis.T

text: Universite de Poitiers

text: public

characteristic: SRA accession

text: Mis.T

text: common pillbug

characteristic: common name

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Hindgut

text: Mis.T


Mis.F

characteristic: ENA-CHECKLIST

text: Universite de Poitiers

text: public

text: Mis.F

characteristic: Submitter Id

text: common pillbug

text: France

text: Armadillidium vulgare

text: Plaine Mothaise

text: Armadillidium vulgare

text: Armadillidium vulgare

text: Faeces

text: Mis.F


text: iPSC

characteristic: ArrayExpress-CellType

text: male

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: GeCKO v2 library A, reference population

characteristic: sample description

text: Homo sapiens


text: iPSC

text: male

text: Homo sapiens

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: GeCKO v2 library A, CXCR4 Positive

text: Homo sapiens


text: iPSC

text: male

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: GeCKO v2 library A, CXCR4 Negative

text: Homo sapiens


text: iPSC

text: male

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: GeCKO v2 library B, reference population

text: Homo sapiens


text: iPSC

text: male

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: GeCKO v2 library B, CXCR4 positive

text: Homo sapiens


text: iPSC

text: male

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: GeCKO v2 library B, CXCR4 Negative

text: Homo sapiens


text: iPSC

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: LentiGuide A plasmid pool

text: Homo sapiens


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: LentiGuide B plasmid pool

text: Homo sapiens


text: Danio rerio

text: SC

text: public

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Mouse

characteristic: ArrayExpress-Phenotype

text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: INSDC last update

text: public

tag: Namespace:School of Agriculture, Policy and Development University of Reading, UK

text: ArrayExpress

characteristic: broker name

text: coconut palm

text: Protocols: Microspore derived embryos (MDE) were collected from in vitro grown anthers. MDE were induced on Eeuwens Y3 (Karunaratne et al. 1985) medium supplemented with 100 micromolar 2, 4-dichlorophenoxyacetic acid and 100 micromolar 1-naphthalene acetic acid (Perera et al. 2008). Total RNA was extracted using an RNeasy Plant Mini Kit (Qiagen, UK) using manufacturers protocol. cDNA libraries were constructed using SMART approach as described by Zhu et al. 2001 using the the oligonucleotides SMART Sfi1A : 5prime- AAG CAG TGG TAT CAA CGC AGA GTG GCC ATT ACG GCCrGrGrG-3', CDS-Sfi1B : AAG CAG TGG TAT CAA CGC AGA GTG GCC GAG GCG GCCd(T)20-3', SMART PCR primer : 5'-AAG CAG TGG TAT CAA CGC AGA GT- 3'. The amplified cDNA PCR products were purified using QIAquick PCR Purification Kit (QIAGEN, CA) and a final yield of 3 microgram was prepared for each tissue type.

characteristic: description

text: Microspore derived embryos

characteristic: embryo classification

text: Sri Lanka Tall variety

characteristic: genotype

text: Cocos nucifera

text: Cocos nucifera L.

text: Embryo

characteristic: organism part

text: Cocos nucifera

text: frozen specimen

characteristic: specimen with known storage state

text: MDE

characteristic: title