text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


status: PUBLIC

text: Danio rerio

tag: attribute

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for dnmt4, allele sa15458,ZMP phenotype 88 clutch 1. A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph88

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TTCCTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TAGTGACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TACAGGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TCCTCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TAGTCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TTCCATTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TCGAAGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TAACGCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TTGGTATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TGAACTGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TACTTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TCTCACGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TCAGGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TAAGTTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TCCAGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wildtype embryo for kdm2aa, allele sa898,ZMP phenotype 86 clutch 1. A 8 base indexing sequence (TGTATGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph86

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (CGATGTTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TTAGGCAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TGACCACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (ACAGTGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (GCCAATGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (CAGATCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (ACTTGATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (GATCAGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TAGCTTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (GGCTACAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped homozygous mutant embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (CTTGTACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TGGTTGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TCTCGGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TAAGCGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TCCGTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TGTACCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped heterozygous embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TTCTGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TCTGCTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TTGGAGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TCGAGCGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TGATACGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TGCATAGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from single genotyped wild type embryo for kdm2aa, allele sa9360,ZMP phenotype 97 clutch 1. A 8 base indexing sequence (TTGACTCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph97

text: Danio rerio

text: mixed


text: Mus Musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: mESC

text: Mus musculus


text: EBI

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: fruit fly

text: Protocols: Kc167 and S2R+ cells (obtained from the Drosophila Genomics Resource Centre [DGRC]) were grown in Schneiders Insect Cell Medium (GIBCO) supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (Thermoscientific). Cell lines were kept at 25C. For Groucho oligomerization experiments cells were cotransfected with dsRNA against the 3UTR of Gro as well as plasmids containing either Gro-GFP or GroL38D;L87D-GFP. Transfections were performed using Effectene according to manufacturers instructions (Qiagen). 300-600ng DNA and 1 microgram of dsRNA were cotransfected per 1-3 x 10^6 cells. Successfully transfected cells were processed six days post transfection. For RNA-seq experiments total RNA was obtained from approximately 5 x 10^6 cells per sample using the Qiagen RNeasy mini kit. mRNA was then extracted using the Dynabeads mRNA Purification Kit (Life Technologies). 50-250 ng of mRNA was used to generate an mRNA library following manufacturers instructions (NEBnext mRNA Library Prep Master Mix _ E6110S). cDNA fragments of 250-350 bp were selected by agarose gel extraction and amplified with 12 cycles of PCR. Resulting libraries were assessed for concentration and size using the Qubit fluorometer and the Agilent2100 Bioanalyzer.

characteristic: description

text: gro38,87-GFP and gro RNA

characteristic: genetic modification

text: gro38,87-GFP and gro RNA

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: E-MTAB-2316_newruns:S2-RNAseq-RNAigro-rep2

text: ArrayExpress

text: fruit fly

text: Protocols: Kc167 and S2R+ cells (obtained from the Drosophila Genomics Resource Centre [DGRC]) were grown in Schneiders Insect Cell Medium (GIBCO) supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (Thermoscientific). Cell lines were kept at 25C. For Groucho knockdown, cells were transfected with dsRNA against the 3UTR of Gro. Transfections were performed using Effectene according to manufacturers instructions (Qiagen). 1 microgram of dsRNA was transfected per 1-3 x 10^6 cells. Successfully transfected cells were processed six days post transfection. For RNA-seq experiments total RNA was obtained from approximately 5 x 10^6 cells per sample using the Qiagen RNeasy mini kit. mRNA was then extracted using the Dynabeads mRNA Purification Kit (Life Technologies). 50-250 ng of mRNA was used to generate an mRNA library following manufacturers instructions (NEBnext mRNA Library Prep Master Mix _ E6110S). cDNA fragments of 250-350 bp were selected by agarose gel extraction and amplified with 12 cycles of PCR. Resulting libraries were assessed for concentration and size using the Qubit fluorometer and the Agilent2100 Bioanalyzer.

text: gro RNA

text: gro RNA

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2316_newruns:S2-RNAseq-groGFP-rep2

text: ArrayExpress

text: fruit fly

text: gro-GFP and gro RNA

text: gro-GFP and gro RNA

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: EBI

text: public

characteristic: SRA accession

text: E-MTAB-2316_newruns:S2-RNAseq-mutGFP-rep2

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

characteristic: common name

text: gro38,87-GFP and gro RNA

text: gro38,87-GFP and gro RNA

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: Drosophila melanogaster


text: EBI

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: fruit fly

text: gro-GFP and gro RNA

text: gro-GFP and gro RNA

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: EBI

text: public

text: E-MTAB-2316_newruns:S2-RNAseq-wildtype-rep2

text: ArrayExpress

text: fruit fly

text: Protocols: Kc167 and S2R+ cells (obtained from the Drosophila Genomics Resource Centre [DGRC]) were grown in Schneiders Insect Cell Medium (GIBCO) supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (Thermoscientific). Cell lines were kept at 25C. They were grown to approximately 70% confluence prior to treatment. For RNA-seq experiments total RNA was obtained from approximately 5 x 10^6 cells per sample using the Qiagen RNeasy mini kit. mRNA was then extracted using the Dynabeads mRNA Purification Kit (Life Technologies). 50-250 ng of mRNA was used to generate an mRNA library following manufacturers instructions (NEBnext mRNA Library Prep Master Mix _ E6110S). cDNA fragments of 250-350 bp were selected by agarose gel extraction and amplified with 12 cycles of PCR. Resulting libraries were assessed for concentration and size using the Qubit fluorometer and the Agilent2100 Bioanalyzer.

characteristic: description

text: wild type genotype

characteristic: genetic modification

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: EBI

text: public

text: ArrayExpress

text: fruit fly

text: gro38,87-GFP and gro RNA

text: gro38,87-GFP and gro RNA

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RNAseq-mutGFP

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: ArrayExpress

text: fruit fly

text: wild type genotype

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: ArrayExpress

text: fruit fly

text: gro-GFP and gro RNA

text: gro-GFP and gro RNA

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster

text: RNAseq-groGFP


text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fruit fly

text: gro RNA

text: gro RNA

text: Drosophila melanogaster

text: Drosophila melanogaster

text: Drosophila melanogaster


text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum


text: EBI

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: ChIP-seq with N499 antibody targeting GR, in a cell line expressing only GR gamma isoform, 1 micromolar dexamethasone for 90 minutes Protocols: Approximately 10 million cells were treated with 0.01% ethanol vehicle or 1 uM dexamethasone for 1.5 h. Chromatin was sheared with a Bio-ruptor water bath sonicator (Diagenode) to produce fragments of 100-200 bp. Protein-G coupled magnetic beads (Active- Motif) were preincubated for 1 h with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4C. Subsequently beads were washed three times with 10 mM TrisaHCl pH 8.0, 1 mM EDTA, 500 mM NaCl, 5% (vol/vol) glycerol, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/uL BSA, followed by three additional washes with 20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% Nonidet P-40, and 0.5% sodium deoxycholate.

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

type: has member


text: EBI

text: public

text: ArrayExpress

text: human

text: Input, 1 micromolar dexamethasone for 90 minutes Protocols: Approximately 10 million cells were treated with 0.01% ethanol vehicle or 1 uM dexamethasone for 1.5 h. Chromatin was sheared with a Bio-ruptor water bath sonicator (Diagenode) to produce fragments of 100-200 bp. Protein-G coupled magnetic beads (Active- Motif) were preincubated for 1 h with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4C. Subsequently beads were washed three times with 10 mM TrisaHCl pH 8.0, 1 mM EDTA, 500 mM NaCl, 5% (vol/vol) glycerol, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/uL BSA, followed by three additional washes with 20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% Nonidet P-40, and 0.5% sodium deoxycholate.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Input


text: EBI

text: public

text: ArrayExpress

text: human

text: ChIP-seq with N499 antibody targeting GR, in a cell line expressing only GR alpha isoform, 1 micromolar dexamethasone for 90 minutes Protocols: Approximately 10 million cells were treated with 0.01% ethanol vehicle or 1 uM dexamethasone for 1.5 h. Chromatin was sheared with a Bio-ruptor water bath sonicator (Diagenode) to produce fragments of 100-200 bp. Protein-G coupled magnetic beads (Active- Motif) were preincubated for 1 h with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4C. Subsequently beads were washed three times with 10 mM TrisaHCl pH 8.0, 1 mM EDTA, 500 mM NaCl, 5% (vol/vol) glycerol, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/uL BSA, followed by three additional washes with 20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% Nonidet P-40, and 0.5% sodium deoxycholate.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 2

characteristic: ENA-CHECKLIST

text: EBI

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 2

text: ArrayExpress

text: thale cress

text: Protocols: The WEE1 knockout (wee1-1) and the RNH2A/WEE1 double knockout (rnh2a/wee1-1) seeds were germinated on control medium on a nylon mesh. Transferred 5 d after germination to control medium or medium supplemented with 2 mM HU 24h after the transfer 200 root tips (<2 to 3 mm) were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with the RNeasy plant kit (Qiagen). Illumina True-Seq libraries were generated from cDNA according to the manufacture’s protocol and sequenced on a HiSeq2000.

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

characteristic: organism part

text: Arabidopsis thaliana

characteristic: strain

text: wee1-1/rnh2a - 0mM HU - 2


E-MTAB-2590:wee1-1 - 2mM HU - 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: E-MTAB-2590:wee1-1 - 2mM HU - 2

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 2mM HU - 2


E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 1

text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 1

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1/rnh2a - 2mM HU - 1


E-MTAB-2590:wee1-1 - 2mM HU - 3

text: EBI

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2590:wee1-1 - 2mM HU - 3

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 2mM HU - 3


E-MTAB-2590:wee1-1 - 0mM HU - 2

text: EBI

text: public

characteristic: SRA accession

text: E-MTAB-2590:wee1-1 - 0mM HU - 2

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 0mM HU - 2


E-MTAB-2590:wee1-1 - 2mM HU - 1

text: EBI

text: public

text: E-MTAB-2590:wee1-1 - 2mM HU - 1

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 2mM HU - 1


E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 2

text: EBI

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 2

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1/rnh2a - 2mM HU - 2


E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 3

text: EBI

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 3

text: ArrayExpress

text: thale cress

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1/rnh2a - 0mM HU - 3


E-MTAB-2590:wee1-1 - 0mM HU - 3

text: EBI

text: public

text: E-MTAB-2590:wee1-1 - 0mM HU - 3

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 0mM HU - 3

characteristic: title


E-MTAB-2590:wee1-1 - 0mM HU - 1

characteristic: ENA-CHECKLIST

text: EBI

text: public

text: E-MTAB-2590:wee1-1 - 0mM HU - 1

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

text: wee1-1 - 0mM HU - 1


E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 1

text: EBI

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 0mM HU - 1

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

characteristic: organism part

text: Arabidopsis thaliana

text: wee1-1/rnh2a - 0mM HU - 1


E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 3

text: EBI

text: public

text: E-MTAB-2590:wee1-1/rnh2a - 2mM HU - 3

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root tip

text: Arabidopsis thaliana

characteristic: strain

text: wee1-1/rnh2a - 2mM HU - 3


text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: DNA from MEF

characteristic: sample description

text: Mus musculus


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: DNA from MEF

text: Mus musculus


text: female

text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: DNA from ESC

text: Mus musculus


text: Plasmodium falciparum

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum


text: 12 weeks

characteristic: ArrayExpress-Age

text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 GFP tagged

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: crosslinked and sonicated chromatin= Input from GFP high sample (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: 12 weeks

text: Glia

text: Adult

text: MeCP2 GFP tagged

text: Brain

text: wildtype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: Bl6 MeCP2 GFP

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: crosslinked and sonicated chromatin= Input from GFP low sample (MeCP2 GFP from 15 BL 6 male)

characteristic: sample description

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: strain


text: 12 weeks

text: Liver

text: Adult

text: MeCP2 GFP tagged

text: Genomic

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: crosslinked and sonicated chromatin= Input from LIVER (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: 12 weeks

characteristic: ArrayExpress-Age

text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 GFP tagged

characteristic: ArrayExpress-Genotype

text: Chromotek GFP trap Agarose

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: ChIP DNA from GFP high neurons (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: 12 weeks

text: Glia

text: Adult

text: MeCP2 GFP tagged

text: Chromotek GFP trap Agarose

text: Brain

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: Bl6 MeCP2 GFP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: ChIP DNA from GFP low glia (MeCP2 GFP from 15 BL 6 male)

characteristic: sample description

text: Mus musculus

text: Bl6 MeCP2 GFP


text: 13 weeks

text: Glia

text: Adult

text: MeCP2 GFP tagged

text: Chromotek GFP trap Agarose

text: Brain

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: strain


text: 14 weeks

characteristic: ArrayExpress-Age

text: Liver

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 GFP tagged

characteristic: ArrayExpress-Genotype

text: Chromotek GFP trap Agarose

characteristic: ArrayExpress-Immunoprecipitate

text: Genomic

characteristic: ArrayExpress-OrganismPart

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: ChIP DNA from LIVER (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: ca 4hr, 0.1ug CT

characteristic: sample description

text: Mus musculus


text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: ca 4hr 1ug CT

text: Mus musculus


text: EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: induced pluripotent stem cell

characteristic: cell type

text: pig

text: LIF dependent pig iPS in mouse ESC Protocols: The piPS cells were maintained on mitotically inactive mouse embryonic fibroblasts (MEFs) feeder derived from ICR mice supplemented with 10% FBS, sodium pyruvate, nonessential amino acids, mercaptoethanol, and LIF, bFGF. For immunoprecipitation, the DNA was first denatured, then immunoprecipitated with the 5mC antibody using the Magnetic Methylated DNA Immunoprecipitation kit (Diagenod). Genomic DNA was extracted from the cell pellets using the DNeasy Mini Kit (Qiagen). The gDNA was sonicated to 100-500 bp and repaired to contain a 3-dA overhang; adapters were then ligated to the end of the DNA fragments according to the Paired-End DNA Sample Prep Kit (Illumina). Real time-PCR was performed to validate the enrichment efficiency. Next, the immunoprecipitated DNA was amplified for approximately 12-15 cycles, and fragments of the proper size (usually 200-300 bp) were gel-purified using the Gel Extraction Kit (Qiagen) and quantified using a 2100 Bioanalyzer (Agilent Technologies).

characteristic: description

text: media

characteristic: growth condition

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa

text: iPS-j

characteristic: title


text: Normal

characteristic: ArrayExpress-Phenotype

text: Mouse

characteristic: ArrayExpress-Species

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Plasmodium vivax

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax


text: Plasmodium falciparum

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: ArrayExpress-DevelopmentalStage

text: C57BL/6N / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus

characteristic: strain


text: C57BL/6N / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (CGATGTTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTAGGCAT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: C57BL/6N / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

text: SC

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGACCACT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (ACAGTGGT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GCCAATGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (CAGATCTG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: C57BL/6N / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Mus musculus

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (ACTTGATG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GATCAGCG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAGCTTGT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GGCTACAG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


characteristic: ArrayExpress-DevelopmentalStage

text: Pitrm1 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (CTTGTACT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGGTTGTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCTCGGTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAAGCGTT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Pitrm1 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCCGTCTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Pitrm1 / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTACCTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Pitrm1 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTCTGTGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCTGCTGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Pitrm1 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTGGAGGT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: southeastern Asian house mouse

text: Mus musculus castaneus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCGAGCGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus castaneus

characteristic: strain


text: Pitrm1 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: western European house mouse

text: Mus musculus domesticus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGATACGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus domesticus


text: Ctr9 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Ctr9 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTCCTGCT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAGTGACT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Ctr9 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TACAGGAT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Ctr9 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCCTCAAT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Ctr9 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAGTCTTG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTCCATTG) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCGAAGTG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


characteristic: ArrayExpress-DevelopmentalStage

text: Ctr9 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTGGTATG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAACGCTG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: female

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGAACTGG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Whole embryo

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TACTTCGG) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Ctr9 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCTCACGG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Ctr9 / C57BL/6N

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCAGGAGG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Ctr9 / C57BL/6N

text: Whole embryo

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAAGTTCG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Ctr9 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCCAGTCG) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Ctr9 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Ctr9tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Ctr9 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTATGCG) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 6 weeks

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: A. thaliana plant 1 RNA. Small RNA libraries were generated by Illumina TruSeq v2 after FDFSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

characteristic: description

text: wild type genotype

characteristic: genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: inflorescence

characteristic: organism part

text: Arabidopsis thaliana

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: 6 weeks

text: ArrayExpress

text: thale cress

text: A. thaliana plant 2 RNA. Small RNA libraries were generated by Illumina TruSeq v2 NSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: inflorescence

text: Arabidopsis thaliana


text: EBI

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ArrayExpress

text: fruit fly

characteristic: common name

text: D. melanogaster mixed sex adult RNA sample. Small RNA libraries were generated by Illumina TruSeq v2 after FDFSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

text: Drosophila melanogaster

text: Oregon-R


text: EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fruit fly

text: D. melanogaster mixed sex adult RNA sample. Small RNA libraries were generated by Illumina TruSeq v2 after NSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

text: Drosophila melanogaster

text: Oregon-R


text: EBI

text: public

text: 6 weeks

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: A. thaliana plant 1 RNA. Small RNA libraries were generated by Illumina TruSeq v2, NSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

characteristic: description

text: wild type genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: inflorescence

text: Arabidopsis thaliana


text: EBI

text: public

text: ArrayExpress

text: house mouse

text: M. musculus testis 25 days post partum RNA sample. Small RNA libraries were generated by Illumina TruSeq v2 after FDFSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: testis

characteristic: organism part

text: Mus musculus


characteristic: ENA-CHECKLIST

text: EBI

text: public

text: 6 weeks

text: ArrayExpress

text: thale cress

text: A. thaliana plant 2 RNA. Small RNA libraries were generated by Illumina TruSeq v2 after FDFSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: inflorescence

text: Arabidopsis thaliana

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: ArrayExpress

text: house mouse

text: M. musculus testis 25 days post partum RNA sample. Small RNA libraries were generated by Illumina TruSeq v2 after NSS treatment Protocols: Total RNA was extracted from N. benthamiana tissue based on the White and Kaper protocol (/White, J.L., and Kaper, J.M. (1989). A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23, 83 to 93). Total RNA from the all other organisms (A. thaliana, M. musculus, C. elegans, D. melanogaster) were extracted by TRIzol reagent (Life Technolgies). To make the small RNA libraries, this total RNA was used directly for Illumina TruSeq small RNA cloning protocol (RS-200-9002DOC) (NSS= non size selected). Or was subject to this protocol after small RNA size selection, by extraction of the 18-30nt band from a denaturing (formamide) polyacrylamide gel (FSS) or after small RNA size selection by extraction of the 18-30nt band from a fully denaturing formaldehyde polyacrylamide gel (FDFSS - described in detail by Harris et al,. in prep).

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: testis

text: Mus musculus


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum


text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-2700:iCLIP_T7-A3G_CEM-SS_HIV_Producer_Cell_Hs_NNNGGCGNN_20110921_LUe40_3

text: ArrayExpress

text: CEM-SS

characteristic: cell line

text: T cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: CEM-SS T cell lines were grown in RPMI media supplemented with foetal bovine serum and Pen/Strept. HIV-1 viruses were produced from CEM-SS T cell lines by infecting cells with delta Vif IIIB strain HIV-1. HIV-1 viruses produced from CEM-SS T cell lines over expressing proteins of interest were concentrated and purified by ultracentrfugation through a sucrose gradient. Producer cells were washed throughly. Viruses or cells were then ressuspended in PBS and subjected to UV-C irradiation. Cells or viruses were ressuspended in 1mL of lysis buffer (50mM Tris-HCL, pH 7.4; 100mM NaCl; 1% NP-40; 0.1% SDS; 0.5% Sodium deoxycholate and protease inhibitor) and sonicated. 0.04ng of RNase A were added to the samples. RNAs cross-linked to proteins were extracted by immunoprecipitation of the proteins of interest iCLIP protocol was followed to isolate RNA bound to proteins of interest and amplify cDNA library. Proteins of interest were immunoprecipitated using anti-T7 or anti-GFP antibodies.. In order to differentiate between identical reads generated by PCR or distinct reads that were immunoprecipitated but that have the same sequence and length , we inserted 2 random nuclotides 5 prime of the barcode and 3 random nucleotides 3 prime of the barcode on each of the primers used to generate cDNA. Reads that have the same random nucleotides were judged to be artifacts from the PCR. This approach has been described in detail in Konig, et al, (2010): iCLIP reveals the function of hnRNP particles in splicing at individual nucleotide resolution, Nat Struct Mol Biol 17:909-915.. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3

text: T7-A3G overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state


characteristic: ENA-CHECKLIST

text: EBI

characteristic: INSDC last update

text: public

text: E-MTAB-2700:iCLIP_T7-3F_CEM-SS_HIV_Producer_Cell_Hs_NNNGGTCNN_20110921_LUe39_4

text: ArrayExpress

characteristic: broker name

text: CEM-SS

text: T cell

text: human

characteristic: description

text: T7-A3F overexpression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: E-MTAB-2700:iCLIP_GFP-A3F_CEM-SS_HIV_Producer_Cell_Hs_NNNTTAANN_20110921_LUe39_7

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: GFP-A3F overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: title


text: EBI

text: public

characteristic: INSDC status

text: E-MTAB-2700:iCLIP_T7-A3G_CEM-SS_HIV_Producer_Cell_Hs_NNNGGCGNN_20110921_LUe39_3

characteristic: Submitter Id

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: T7-A3G overexpression

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-2700:iCLIP_T7-GFP_CEM-SS_HIV_Producer_Cell_Hs_NNNTTGTNN_20110921_LUe40_2

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: T7-GFP overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: EBI

text: public

text: E-MTAB-2700:iCLIP_GFP-A3G_CEM-SS_HIV_Producer_Cell_Hs_NNNTGGCNN_20110921_LUe40_6

text: ArrayExpress

text: CEM-SS

characteristic: cell line

text: T cell

characteristic: cell type

text: human

characteristic: common name

text: GFP-A3G overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


characteristic: ENA-CHECKLIST

text: EBI

characteristic: INSDC last update

text: public

text: E-MTAB-2700:iCLIP_GFP_CEM-SS_HIV_Producer_Cell_Hs_NNNCAATNN_20110921_LUe40_5

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: GFP overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: specimen with known storage state


text: EBI

text: public

text: E-MTAB-2700:iCLIP_GFP_CEM-SS_HIV_Producer_Cell_Hs_NNNTTGTNN_20110921_LUe39_5

text: ArrayExpress

characteristic: broker name

text: CEM-SS

text: T cell

text: human

characteristic: description

text: GFP overexpression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: E-MTAB-2700:iCLIP_T7-3F_CEM-SS_HIV_Producer_Cell_Hs_NNNGGTCNN_20110921_LUe40_4

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: T7-A3F overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: EBI

text: public

characteristic: INSDC status

text: E-MTAB-2700:iCLIP_T7-GFP_CEM-SS_HIV_Producer_Cell_Hs_NNNCAATNN_20110921_LUe39_2

characteristic: Submitter Id

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: T7-GFP overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen

characteristic: title


text: EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2700:iCLIP_GFP-A3G_CEM-SS_HIV_Producer_Cell_Hs_NNNAATANN_20110921_LUe39_6

text: ArrayExpress

text: CEM-SS

text: T cell

text: human

text: GFP-A3G overexpression

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: EBI

text: public

characteristic: SRA accession

text: E-MTAB-2700:iCLIP_GFP-A3F_CEM-SS_HIV_Producer_Cell_Hs_NNNCCACNN_20110921_LUe40_7

text: ArrayExpress

text: CEM-SS

characteristic: cell line

text: T cell

characteristic: cell type

text: human

text: GFP-A3F overexpression

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: fresh specimen


text: Mouse

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

text: SC

characteristic: INSDC last update

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: EBI

text: public

text: ArrayExpress

characteristic: broker name

text: lymphocyte of B lineage

text: chicken

text: Protocols: Chicken DT40 cells were grown in RPMI-1640 with 7% FBS, 3% chicken serum and supplemented with pen/strep at 37C with 10% CO2. The source (starting sample for the experiment) were wild type and H3.3 knockout DT40 cells. RNA was extracted using an RNeasy Mini Kit (Qiagen). The RNA quality and quantity was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were constructed by BGI (Beijing Genomics Institute)

characteristic: description

text: wild type genotype

characteristic: genotype

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: public

text: ArrayExpress

text: lymphocyte of B lineage

text: chicken

text: h3.3a-/-, h3.3b-/-

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


E-MTAB-2762:Sample 3

text: EBI

text: public

characteristic: INSDC status

text: E-MTAB-2762:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Silastic tubing containing 35 ul of oil or 35ul of oil with 180ug/ml E2. Implants remained in mice for 7 days. Total RNA was extracted using Ambion RNAqueous according to manufacturers protocol.Samples were treated with Turbo DNA-free reagent to remove genomic DNA. The TruSeq RNA sample prep kit was used according to manufacturers protocol. 1ug total RNA was used to make poly-A selected and barcoded libraries. Libraries were pooled and quantifed using real time PCR and library quantification kit (Kapa Biosystems).

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 3

characteristic: title