text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


status: PUBLIC

text: Hatching:Long pec - ZFS:0000033

tag: attribute

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_119_48hpf_pool1-sc-2152073

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool2-sc-2152074

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool3-sc-2152075

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool4-sc-2152076

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool5-sc-2152077

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool6-sc-2152078

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_119_48hpf_pool7-sc-2152079

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_119_48hpf_pool8-sc-2152080

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool9-sc-2152081

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_119_48hpf_pool10-sc-2152082

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Hatching:Long pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_119_48hpf_pool11-sc-2152083

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at hatching - long pec stage (48 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTCGTGT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAATCTGT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGGTGCT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCATGGCT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTTAGCCT) is bases 13 to 20 of read 1 followed by CG and polyT.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTCGCTAT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGAATGAT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGCCAAT) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCTCCTTG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single wild type zebrafish embryo collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTAAGGTG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool1-sc-2152096

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGGATGG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_120_72hpf_pool2-sc-2152097

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTTGTCGG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_120_72hpf_pool3-sc-2152098

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGATTAGG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool4-sc-2152099

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATAGAGG) is bases 13 to 20 of read 1 followed by CG and polyT.

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_120_72hpf_pool5-sc-2152100

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTGTCG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool6-sc-2152101

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCAATCCG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool7-sc-2152102

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GACCTTAG) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool8-sc-2152103

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCCTGTTC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool9-sc-2152104

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCACTGTC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_120_72hpf_pool10-sc-2152105

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCTAACTC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


text: Larval:Protruding mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_120_72hpf_pool11-sc-2152106

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a pool of eight wild type zebrafish embryos collected at larval-protruding mouth stage (72 hpf) plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATTCATC) is bases 13 to 20 of read 1 followed by CG and polyT.

text: Danio rerio

text: mixed


E-MTAB-3021:Control 2

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3021:Control 2

tag: Namespace:The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: Human Cal51 breast carcinoma cells were treated with either control siRNA or PRPF8 siRNA and harvested 54 hours post-transfection for total RNA. 4 experimental replicates for PRPF8 siRNA and 3 experimental replicates for Control siRNA are provided. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers protocol. Illumina's TruSeq RNA-seq kit was used to prepare libraries for sequencing according to manufacturers protocol.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Control 2

characteristic: title

type: has member


E-MTAB-3021:PRPF8 KD 2

characteristic: External Id

text: The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre

text: public

characteristic: SRA accession

text: E-MTAB-3021:PRPF8 KD 2

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: PRPF8 KD 2


E-MTAB-3021:PRPF8 KD 1

text: public

text: E-MTAB-3021:PRPF8 KD 1

text: ArrayExpress

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: PRPF8 KD 1


E-MTAB-3021:PRPF8 KD 3

text: public

text: E-MTAB-3021:PRPF8 KD 3

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: PRPF8 KD 3


E-MTAB-3021:Control 3

text: public

text: E-MTAB-3021:Control 3

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Control 3


E-MTAB-3021:Control 1

text: public

text: E-MTAB-3021:Control 1

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Control 1


E-MTAB-3021:PRPF8 KD 4

text: public

text: E-MTAB-3021:PRPF8 KD 4

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: PRPF8 KD 4


text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium

text: ArrayExpress

text: thale cress

text: Protocols: RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega) Preparation of six strand-specific cDNA libraries: Purification of poly-A containing mRNA molecules; mRNA fragmentation; Random primed cDNA synthesis (strand specific); Adapter ligation and adapter specific PCR amplification

text: GFP-GR

characteristic: genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Arabidopsis thaliana


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium

text: public

text: ArrayExpress

text: thale cress

text: GFP-GR

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Arabidopsis thaliana


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: Human

text: PhiX DNA spike-in at 50%

characteristic: ArrayExpress-Treatment

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: DNA library from AML cell lines after CRISPR/CAS9 screens

characteristic: sample description

text: Homo sapiens


text: Homo Sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: BAC DNA

text: Homo sapiens


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated Wild Type Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Wild Type Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated Wild Type Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated BAP1 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 BAP1 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 4 Unstimulated BAP1 Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: Plasmodium falciparum

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: strain


text: Plasmodium falciparum

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Plasmodium falciparum

text: (Cambodia)

text: SC

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: (Cambodia)


text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: male

text: Homo Sapien

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


text: Homo Sapiens

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

text: public

text: human

text: Homo sapiens

text: BAC DNA

text: Homo sapiens


text: caecum

characteristic: ArrayExpress-OrganismPart

text: Mus musculus

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: Plasmodium vivax

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax


characteristic: ArrayExpress-CellType

characteristic: ArrayExpress-Immunoprecipitate

text: Blood

text: Cancer

characteristic: ArrayExpress-Phenotype

text: male

text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: ChIP

characteristic: sample description

text: Homo sapiens


text: Blood

text: Cancer

text: female

text: Homo sapiens

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: ChIP

text: Homo sapiens


text: CEBPB

text: Blood

text: Cancer

text: female

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: ChIP

text: Homo sapiens


text: CTCF

text: Blood

text: Cancer

text: female

text: Homo sapiens

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: ChIP

text: Homo sapiens


text: CTCF

text: Blood

characteristic: ArrayExpress-OrganismPart

text: Cancer

text: male

text: Homo sapiens

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

text: public

text: human

text: Homo sapiens

text: ChIP

text: Homo sapiens


text: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: TChAP from cell suspension cultures was done according to Verkest et al. (2014) 35S::PPD2-HBH and wild-type PSB-D TChAP DNA libraries were prepared according to the protocol of Illumina (www.illumina.com/sequencing/)

characteristic: description

text: HBH-tagged PPD2

characteristic: genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Arabidopsis thaliana

characteristic: title


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: control

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Control


text: public

tag: Namespace:Department of Biological Sciences, Division of Genetics and Physiology, University of Turku

text: Tenerife blue tit

characteristic: common name

text: Morocco

characteristic: geographic location (country and/or sea)

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Biological Sciences, Division of Genetics and Physiology, University of Turku

text: public

text: Tenerife blue tit

text: Morocco

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: blue tit

text: Norway

text: Cyanistes caeruleus

characteristic: organism

text: Cyanistes caeruleus

text: Tits


characteristic: ENA-LAST-UPDATE

text: public

text: Tenerife blue tit

text: El Hierro

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


characteristic: INSDC center name

text: public

text: Tenerife blue tit

text: Fuerteventura Lanzarote

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


characteristic: INSDC first public

text: public

text: Tenerife blue tit

text: Gran Canaria

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


characteristic: INSDC last update

text: public

text: Tenerife blue tit

text: La Gomera

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

characteristic: INSDC status

text: Tenerife blue tit

text: La Palma

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: Tenerife

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: blue tit

text: Spain

text: Cyanistes caeruleus

text: Cyanistes caeruleus

text: Tits


text: public

text: azure tit

text: Russia

text: Cyanistes cyanus

text: Cyanistes cyanus

text: Tits


text: public

text: Tenerife blue tit

text: Morocco

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: El Hierro

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: Fuerteventura Lanzarote

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: La Gomera

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: La Palma

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: public

text: Tenerife blue tit

text: Tenerife

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


text: The Roslin Institute

text: public

tag: Namespace:The Roslin Institute

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

characteristic: cell type

text: chicken

text: Protocols: Chicken bone marrow derived macrophage (BMDM) culture: RNA from chicken BMDM, day 7 embryos, or DF-1 cells, was extracted using TRIzol reagent, as described by the manufacturers (Invitrogen). RNA was quantified using a Nanodrop and RNA quality was assessed using Agilent nanochip Samples were prepared for mRNA sequencing using 1ug of total RNA starting material following the Illumina Truseq RNA Sequencing protocol. Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies, South Queensferry, UK) and then clustered onto a paired read flowcell using the Illumina v2 cluster generation kit at a 8pM concentration.

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus

text: colony-stimulating factor 1 (CSF-1)

characteristic: stimulus


text: The Roslin Institute

text: public

text: ArrayExpress

text: embryonic cell

text: chicken

characteristic: description

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus

characteristic: title


characteristic: External Id

text: The Roslin Institute

text: public

characteristic: SRA accession

text: ArrayExpress

text: DF-1 cell

text: chicken

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


text: public

characteristic: Submitter Id

text: blue tit

characteristic: common name

text: Norway

text: Cyanistes caeruleus

text: Cyanistes caeruleus

text: Tits


characteristic: ENA-CHECKLIST

text: public

text: Tenerife blue tit

text: Gran Canaria

characteristic: geographic location (country and/or sea)

text: Cyanistes teneriffae

text: Cyanistes teneriffae

text: Tits


characteristic: ENA-FIRST-PUBLIC

text: public

text: Tenerife blue tit

text: Libya

text: Cyanistes teneriffae

characteristic: organism

text: Cyanistes teneriffae

text: Tits


E-MTAB-3051:Sample 30

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 30

tag: Namespace:Kings College London

text: 73 year

characteristic: age

characteristic: bmi

text: ArrayExpress

text: human

text: Protocols: Peripheral blood samples is collected during visit and DNA is extracted and stored -80oC for sequencing Standard library construction

text: type II diabetes mellitus

characteristic: disease

characteristic: family

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

characteristic: sex

text: Sample 30


E-MTAB-3051:Sample 15

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 15

text: 60 year

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 15

characteristic: title


E-MTAB-3051:Sample 18

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 18

characteristic: Submitter Id

text: 64 year

text: ArrayExpress

text: human

characteristic: common name

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 18


E-MTAB-3051:Sample 33

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 33

text: 67 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 33


E-MTAB-3051:Sample 6

text: Kings College London

text: public

text: E-MTAB-3051:Sample 6

text: 47 year

characteristic: bmi

text: ArrayExpress

text: human

text: type II diabetes mellitus

characteristic: disease

characteristic: family

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

characteristic: sex

text: Sample 6


E-MTAB-3051:Sample 26

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 26

text: 42 year

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 26


E-MTAB-3051:Sample 3

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 3

characteristic: Submitter Id

text: 65 year

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 3

characteristic: title


E-MTAB-3051:Sample 50

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 50

text: 76 year

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 50


E-MTAB-3051:Sample 24

text: Kings College London

text: public

text: E-MTAB-3051:Sample 24

text: 66 year

characteristic: bmi

text: ArrayExpress

text: human

text: type II diabetes mellitus

characteristic: disease

characteristic: family

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 24


E-MTAB-3051:Sample 21

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 21

text: 45 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 21


E-MTAB-3051:Sample 52

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 52

characteristic: Submitter Id

text: 74 year

text: ArrayExpress

text: human

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 52


E-MTAB-3051:Sample 41

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 41

text: 73 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

text: Sample 41

characteristic: title


E-MTAB-3051:Sample 36

text: Kings College London

text: public

text: E-MTAB-3051:Sample 36

text: 54 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

text: type II diabetes mellitus

characteristic: disease

characteristic: family

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: male

text: Sample 36


E-MTAB-3051:Sample 5

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 5

text: 47 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-3051:Sample 22

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 22

characteristic: Submitter Id

text: 45 year

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 22


E-MTAB-3051:Sample 39

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 39

text: 59 year

characteristic: age

text: ArrayExpress

text: human

characteristic: description

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 39


E-MTAB-3051:Sample 46

text: Kings College London

text: public

text: E-MTAB-3051:Sample 46

text: 59 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

text: normal

characteristic: disease

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 46

characteristic: title


E-MTAB-3051:Sample 53

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 53

text: 53 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: male

text: Sample 53


E-MTAB-3051:Sample 28

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 28

characteristic: Submitter Id

text: 61 year

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 28


E-MTAB-3051:Sample 23

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 23

text: 66 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 23


E-MTAB-3051:Sample 42

text: Kings College London

text: public

text: E-MTAB-3051:Sample 42

text: 73 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

text: Sample 42


E-MTAB-3051:Sample 43

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 43

text: 56 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

text: Sample 43

characteristic: title


E-MTAB-3051:Sample 7

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 7

characteristic: Submitter Id

text: 79 year

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 7


E-MTAB-3051:Sample 40

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 40

text: 59 year

characteristic: age

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 40


E-MTAB-3051:Sample 9

text: Kings College London

text: public

text: E-MTAB-3051:Sample 9

text: 74 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 9


E-MTAB-3051:Sample 11

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 11

text: 80 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 11


E-MTAB-3051:Sample 20

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 20

characteristic: Submitter Id

text: 53 year

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 20

characteristic: title


E-MTAB-3051:Sample 31

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 31

text: 61 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 31


E-MTAB-3051:Sample 51

text: Kings College London

text: public

text: E-MTAB-3051:Sample 51

text: 74 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 51


E-MTAB-3051:Sample 37

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 37

text: 70 year

text: ArrayExpress

characteristic: broker name

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 37


E-MTAB-3051:Sample 29

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 29

characteristic: Submitter Id

text: 73 year

text: ArrayExpress

text: human

text: normal

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 29


E-MTAB-3051:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 16

text: 60 year

characteristic: age

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 16

characteristic: title


E-MTAB-3051:Sample 38

text: Kings College London

text: public

text: E-MTAB-3051:Sample 38

text: 70 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: type II diabetes mellitus

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 38


E-MTAB-3051:Sample 2

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 2

text: 65 year

text: ArrayExpress

characteristic: broker name

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 2


E-MTAB-3051:Sample 49

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 49

characteristic: Submitter Id

text: 75 year

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 49


E-MTAB-3051:Sample 27

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 27

text: 61 year

characteristic: age

text: ArrayExpress

text: human

text: normal

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 27


E-MTAB-3051:Sample 1

text: Kings College London

text: public

text: E-MTAB-3051:Sample 1

text: 65 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 1

characteristic: title


E-MTAB-3051:Sample 19

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 19

text: 53 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 19


E-MTAB-3051:Sample 44

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 44

characteristic: Submitter Id

text: 56 year

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: male

text: Sample 44


E-MTAB-3051:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 10

text: 72 year

characteristic: age

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 10


E-MTAB-3051:Sample 4

text: Kings College London

text: public

text: E-MTAB-3051:Sample 4

text: 65 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: type II diabetes mellitus

characteristic: disease

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 4


E-MTAB-3051:Sample 47

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 47

text: 63 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 47

characteristic: title


E-MTAB-3051:Sample 13

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 13

characteristic: Submitter Id

text: 81 year

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 13


E-MTAB-3051:Sample 12

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 12

text: 80 year

characteristic: age

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 12


E-MTAB-3051:Sample 35

text: Kings College London

text: public

text: E-MTAB-3051:Sample 35

text: 54 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: type II diabetes mellitus

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

characteristic: sex

text: Sample 35


E-MTAB-3051:Sample 8

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 8

text: 79 year

text: ArrayExpress

characteristic: broker name

text: human

text: type II diabetes mellitus

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 8


E-MTAB-3051:Sample 34

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 34

characteristic: Submitter Id

text: 67 year

text: ArrayExpress

text: human

text: type II diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 34

characteristic: title


E-MTAB-3051:Sample 17

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 17

text: 64 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 17


E-MTAB-3051:Sample 54

text: Kings College London

text: public

text: E-MTAB-3051:Sample 54

text: 53 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: male

text: Sample 54


E-MTAB-3051:Sample 45

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 45

text: 59 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 45


E-MTAB-3051:Sample 14

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Kings College London

text: public

characteristic: SRA accession

text: E-MTAB-3051:Sample 14

characteristic: Submitter Id

text: 81 year

text: ArrayExpress

text: human

text: type II diabetes mellitus

characteristic: family

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 14


E-MTAB-3051:Sample 25

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Kings College London

characteristic: INSDC center name

text: public

text: E-MTAB-3051:Sample 25

text: 42 year

characteristic: age

text: ArrayExpress

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 25

characteristic: title


E-MTAB-3051:Sample 32

text: Kings College London

text: public

text: E-MTAB-3051:Sample 32

text: 61 year

characteristic: bmi

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: type II diabetes mellitus

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 32


E-MTAB-3051:Sample 48

text: Kings College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-3051:Sample 48

text: 63 year

text: ArrayExpress

characteristic: broker name

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

characteristic: sample name

text: Homo sapiens

text: female

text: Sample 48


text: male

characteristic: ArrayExpress-Sex

text: mus musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: External Id

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: MONOTEMPLTE

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Mus Musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC center name

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Homo Sapien

text: SC

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: Homo sapiens


text: The Roslin Institute

text: public

text: ArrayExpress

characteristic: broker name

text: lung epithelial cell

characteristic: cell type

text: chicken

text: Protocols: Three groups comprising three indigenous, three hybrid and three commercial two day-old chicks were used in the experiment. These were used to obtain airway epithelial cells which were isolated from the trachea and lungs and cultured by the methods described by Zaffuto et al., (2008). The tracheal epithelial cells and lung epithelial cells from the three groups once adopted into culture were split into two groups each having three replicates. One group was infected with a Kenyan virulent strain of Newcastle disease virus with a multiplicity of infection (MOI) of 0.5 and the second group served as control. Both groups were incubated for 36 hours after which total RNA was obtained RNA was obtained after standard Trizol extraction Libraries were prepared as per manufacturer’s instructions for the SOLiD total RNAseq kit

characteristic: description

text: Indigenous

characteristic: ecotype

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: The Roslin Institute

text: public

characteristic: SRA accession

text: ArrayExpress

text: lung epithelial cell

text: chicken

text: Newcastle Disease

characteristic: disease

text: Kenbro

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


characteristic: ENA-FIRST-PUBLIC

text: The Roslin Institute

text: public

characteristic: Submitter Id

text: ArrayExpress

text: trachea epithelial cell

text: chicken

text: Rhode Island Red

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


characteristic: ENA-LAST-UPDATE

text: The Roslin Institute

characteristic: INSDC center name

text: public

text: ArrayExpress

text: trachea epithelial cell

text: chicken

text: Indigenous

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


text: The Roslin Institute

characteristic: INSDC first public

text: public

text: ArrayExpress

text: lung epithelial cell

text: chicken

characteristic: common name

text: Rhode Island Red

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


text: The Roslin Institute

characteristic: INSDC last update

text: public

text: ArrayExpress

text: trachea epithelial cell

text: chicken

text: Newcastle Disease

text: Rhode Island Red

text: Gallus gallus

characteristic: organism

text: Gallus gallus

text: Gallus gallus


text: The Roslin Institute

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: lung epithelial cell

text: chicken

text: Kenbro

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


text: The Roslin Institute

text: public

text: ArrayExpress

text: lung epithelial cell

characteristic: cell type

text: chicken

characteristic: description

text: Newcastle Disease

text: Indigenous

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


text: The Roslin Institute

text: public

text: ArrayExpress

text: trachea epithelial cell

text: chicken

text: Newcastle Disease

text: Indigenous

characteristic: ecotype

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: The Roslin Institute

text: public

text: ArrayExpress

text: lung epithelial cell

text: chicken

text: Newcastle Disease

text: Rhode Island Red

text: Gallus gallus

text: Gallus gallus

text: Gallus gallus


E-MTAB-3037:Sample 15

text: public

characteristic: SRA accession

text: E-MTAB-3037:Sample 15

tag: Namespace:Laboratory of Genetics - Gage The Salk Institute for Biological Studies

text: 45 year

characteristic: age

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X45yr_E5__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 15


E-MTAB-3037:Sample 18

characteristic: ENA-FIRST-PUBLIC

text: Laboratory of Genetics - Gage The Salk Institute for Biological Studies

text: public

text: E-MTAB-3037:Sample 18

characteristic: Submitter Id

text: 55 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X55yr_E10__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 18


E-MTAB-3037:Sample 85

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: E-MTAB-3037:Sample 85

text: 86 year

text: ArrayExpress

text: Human post-mortem prefrontal cortex

text: human

text: X86yr_Ag13_PFC Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 85


E-MTAB-3037:Sample 76

characteristic: INSDC first public

text: public

text: E-MTAB-3037:Sample 76

text: 15 year

text: ArrayExpress

text: Human post-mortem prefrontal cortex

text: human

characteristic: common name

text: X15yr_Ag4_PFC Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 76


E-MTAB-3037:Sample 3

characteristic: INSDC last update

text: public

text: E-MTAB-3037:Sample 3

text: 1 year

text: ArrayExpress

text: iN conversion time point

text: human

text: X1yr_O6_12d Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 3


E-MTAB-3037:Sample 26

text: public

characteristic: INSDC status

text: E-MTAB-3037:Sample 26

text: 0 year

text: ArrayExpress

characteristic: broker name

text: Purified induced neurons (iN)

text: human

text: X0yr_BJ__iN Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 26


E-MTAB-3037:Sample 50

text: public

text: E-MTAB-3037:Sample 50

text: 0 year

text: ArrayExpress

text: Human iPSCs

characteristic: cell type

text: human

text: X0yr_GM_c2_iPS Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 50


E-MTAB-3037:Sample 60

text: public

text: E-MTAB-3037:Sample 60

text: 45 year

text: ArrayExpress

text: Human iPSCs

text: human

text: X45yr_E5_iPS Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 60

characteristic: title


E-MTAB-3037:Sample 24

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-3037:Sample 24

text: 87 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X87yr_A84__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 24


E-MTAB-3037:Sample 21

characteristic: External Id

text: public

text: E-MTAB-3037:Sample 21

text: 69 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X69yr_E7_F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 21


E-MTAB-3037:Sample 61

text: public

characteristic: SRA accession

text: E-MTAB-3037:Sample 61

text: 45 year

text: ArrayExpress

text: Human iPSCs

text: human

text: X45yr_ZER_iPS Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 61


E-MTAB-3037:Sample 62

text: public

text: E-MTAB-3037:Sample 62

text: 55 year

characteristic: age

text: ArrayExpress

text: Human iPSCs

text: human

text: X55yr_E10_iPS Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 62


E-MTAB-3037:Sample 39

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-3037:Sample 39

text: 55 year

text: ArrayExpress

text: Purified induced neurons (iN)

text: human

text: X55yr_E10__iN Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 39


E-MTAB-3037:Sample 22

text: public

text: E-MTAB-3037:Sample 22

characteristic: Submitter Id

text: 71 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X71yr_E30__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 22


E-MTAB-3037:Sample 28

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-3037:Sample 28

text: 0 year

text: ArrayExpress

text: Purified induced neurons (iN)

text: human

text: X0yr_GM_d2_iN Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 28


E-MTAB-3037:Sample 70

characteristic: INSDC center name

text: public

text: E-MTAB-3037:Sample 70

text: 87 year

text: ArrayExpress

text: Human iPSCs

text: human

text: X87yr_A84_c2_iPS Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 70


E-MTAB-3037:Sample 7

characteristic: INSDC first public

text: public

text: E-MTAB-3037:Sample 7

text: 0 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X0yr_BJ__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 7


E-MTAB-3037:Sample 40

text: Laboratory of Genetics - Gage The Salk Institute for Biological Studies

text: public

text: E-MTAB-3037:Sample 40

text: 65 year

text: ArrayExpress

text: Purified induced neurons (iN)

text: human

characteristic: common name

text: X65yr_PET__iN Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 40


E-MTAB-3037:Sample 77

characteristic: INSDC last update

text: public

text: E-MTAB-3037:Sample 77

text: 22 year

text: ArrayExpress

text: Human post-mortem prefrontal cortex

text: human

text: X22yr_Ag5_PFC Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 77


E-MTAB-3037:Sample 11

text: public

text: E-MTAB-3037:Sample 11

text: 29 year

text: ArrayExpress

text: Primary human fibroblasts

text: human

text: X29yr_E1__F Protocols: For the direct conversion of human fibroblasts into induced neurons (iN), cells were lentivirally transduced to express rtTA and a 2A-peptide-linked transcript coding for the pro-neuronal transcription factors Ngn2 and Ascl1, resulting in transgenic, but silent and expandable, AN fibroblasts. For iN conversion, cells were transferred to conversion media containing doxycycline and small molecular enhancers (also see Ladewig et al., Nat Methods 2012). Under these conditions, fibroblasts underwent marked morphological changes into neuronal cells, with typical neuronal morphologies appearing around week 2 of conversion. RNA was collected for RNA-Seq on various time points during the iN conversion process. To analyze the transcriptome of iNs via RNA-Seq, neuronal RNA needs to be pure, with minimal fibroblast contamination that might confound analysis. As live iNs express PSA-NCAM on the cell surface, we established a FACS-based protocol for purification of neurons using a PSA-NCAM antibody. Pure iN RNA for RNA-Seq was isolated by sorting directly into Trizol-LS reagent. All other fibroblast and iPSC RNA samples were directly collected from adherent mono-cultures. Trizol-LS and RNeasy mini TruSeq stranded mRNA (Illumina)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 11