name: E-MTAB-2663:MCF7 + Cisplatin (50µM for 24 hours)

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY

text: public

text: ArrayExpress

text: human

text: Protocols: MCF7 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with non-essential amino-acids (1%), penicillin and streptomycine (1%), gentamycin (0.1%), fungizone (0.1%) and 10% FCS. MCF7 cells were treated with 50µM of cisplatin (cis-diamine platinum(II) dichloride) for 24 hours RNAs were purified from cultured cells using the High Pure RNA isolation kit (Roche). The integrity of RNA was checked with BioAnalyser 2100 (Agilent technologies) that indicated a RQI score >8 The libraries were prepared with Truseq mRNA Sample Prep kit (Illumina) from 1 microgram of total RNA following manufacturer's instructions. mRNAs were isolated by poly-A selection and fragmented (8 minutes at 94°). Fragmented mRNAs were used for reverse-transcription in the presence of Superscript II (Invitrogen) and random primers. After second strand synthesis, end-repair, A-tailing and purification, the double strand cDNA fragments were ligated to Truseq adapters containing the index sequences. Fifteen cycles of PCR in the presence of dedicated PCR primers and PCR master mix were applied to generate the final libraries.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: MCF7 + Cisplatin (50µM for 24 hours)

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Ecole Normale Superieure de Lyon

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Ecole Normale Superieure de Lyon

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: baker's yeast

characteristic: common name

text: Protocols: S.cerevisiae strains used were BY4716 and RM11-1A. Cells were grown to exponential phase in synthetic medium with 2% glucose (SDall) at 30°C ChIP was performed at single-nucleosome resolution using formaldehyde fixation followed by micrococcal nuclease digestion and isolation of 150 bp fragments. Three BY and RM independent cultures were processed for nucleosome mapping (MNase-seq samples). Libraries were built following the procedure of the BioScientific NEXTflex™ ChIP-Seq Kit and NEXTflex™ ChIP-seq Barcodes.

characteristic: description

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain

type: has member


text: Ecole Normale Superieure de Lyon

text: public

text: ArrayExpress

text: baker's yeast

text: Protocols: S.cerevisiae strains used were BY4716 and RM11-1A. Cells were grown to exponential phase in synthetic medium with 2% glucose (SDall) at 30°C ChIP was performed using anti-H3K14ac antibody as described in Nagarajan et al. PLoS Genetics 2010 6(4):e1000913. Libraries were built following the procedure of the BioScientific NEXTflex™ ChIP-Seq Kit and NEXTflex™ ChIP-seq Barcodes.

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

tag: Namespace:College of Veterinary Medicine, Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;

text: ArrayExpress

text: somatic cell

characteristic: cell type

text: pig

text: pig embryo fibroblast Protocols: The RNA library construction and deep sequencing were performed in CapitalBio Corporation (Beijing, China), using the TruSeq Small RNA Sample Preparation Kit and sequencing Kit (Illumina, CA) according to the manufacturer’s protocol. Briefly, 4mg of total RNA was ligated with the 39and 59 adapters successively. After reverse transcription, a 12 cycles of PCR reaction was performed. The resultant library was then qualified by the Agilent 2100 High Sensitivity DNA chip and quantified by Qubit and qPCR. The cluster generation and sequencing were performed on the cBot and Hiseq2000 respectively, following the manufacturer’s standard cBot and sequencing protocols. The adapter sequences were removed from both ends of Solexa reads. A per script was developed to remove the low quality reads, empty tags (adaptor sequence only) and tags less than 18 nt or more than 31 nt. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer’s recommendations and index odes were added to attribute sequences to each sample. Briefly, NEB 3' SR Adaptor was directly and specifically ligated to 3' end of miRNA, siRNA and piRNA. After the 3' ligation reaction, the SR RT Primer hybridized to the excess of 3' SR Adaptor (that remained free after the 3' ligation reaction) and transformed the single-stranded DNA adaptor into a double-stranded DNA molecule. This step is important to prevent adaptor-dimer formation, besides, dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5´ SR Adaptor in the subsequent ligation step. 5´ends adapter was ligated to 5´ends of miRNAs, siRNA and piRNA. Then first strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RNase H-). PCR amplification was performed using LongAmp Taq 2X Master Mix, SR Primer for illumina and index (X) primer. PCR products were purified on a 8% polyacrylamide gel (100V, 80 min). DNA fragments corresponding to 140~160 bp (the length of small noncoding RNA plus the 3' and 5' adaptors) were recovered and dissolved in 8 μL elution buffer. At last, library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500/2000 platform and 50bp single-end reads were generated. Total RNA was isolated from cell cultures or snap-frozen tissues, in biological triplicates or greater, using TRIzol extraction and purified with the Qiagen RNA Purification Kit.

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa

text: PEF


characteristic: ENA-LAST-UPDATE

text: College of Veterinary Medicine, Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;

text: public

text: ArrayExpress

text: stem cell

text: pig

text: The piPSC-g lines derived in our labwith culture media added both bFGF and LIF also showed the flattened morphology Protocols: The RNA library construction and deep sequencing were performed in CapitalBio Corporation (Beijing, China), using the TruSeq Small RNA Sample Preparation Kit and sequencing Kit (Illumina, CA) according to the manufacturer’s protocol. Briefly, 4mg of total RNA was ligated with the 39and 59 adapters successively. After reverse transcription, a 12 cycles of PCR reaction was performed. The resultant library was then qualified by the Agilent 2100 High Sensitivity DNA chip and quantified by Qubit and qPCR. The cluster generation and sequencing were performed on the cBot and Hiseq2000 respectively, following the manufacturer’s standard cBot and sequencing protocols. The adapter sequences were removed from both ends of Solexa reads. A per script was developed to remove the low quality reads, empty tags (adaptor sequence only) and tags less than 18 nt or more than 31 nt. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer’s recommendations and index odes were added to attribute sequences to each sample. Briefly, NEB 3' SR Adaptor was directly and specifically ligated to 3' end of miRNA, siRNA and piRNA. After the 3' ligation reaction, the SR RT Primer hybridized to the excess of 3' SR Adaptor (that remained free after the 3' ligation reaction) and transformed the single-stranded DNA adaptor into a double-stranded DNA molecule. This step is important to prevent adaptor-dimer formation, besides, dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5´ SR Adaptor in the subsequent ligation step. 5´ends adapter was ligated to 5´ends of miRNAs, siRNA and piRNA. Then first strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RNase H-). PCR amplification was performed using LongAmp Taq 2X Master Mix, SR Primer for illumina and index (X) primer. PCR products were purified on a 8% polyacrylamide gel (100V, 80 min). DNA fragments corresponding to 140~160 bp (the length of small noncoding RNA plus the 3' and 5' adaptors) were recovered and dissolved in 8 μL elution buffer. At last, library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500/2000 platform and 50bp single-end reads were generated. Total RNA was isolated from cell cultures or snap-frozen tissues, in biological triplicates or greater, using TRIzol extraction and purified with the Qiagen RNA Purification Kit.

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa

text: iPS-g


text: public

text: ArrayExpress

text: stem cell

text: pig

text: LIF dependent pig iPS cell lines derived in their lab previously (Fujishiro, Nakano et al. 2013) Protocols: The RNA library construction and deep sequencing were performed in CapitalBio Corporation (Beijing, China), using the TruSeq Small RNA Sample Preparation Kit and sequencing Kit (Illumina, CA) according to the manufacturer’s protocol. Briefly, 4mg of total RNA was ligated with the 39and 59 adapters successively. After reverse transcription, a 12 cycles of PCR reaction was performed. The resultant library was then qualified by the Agilent 2100 High Sensitivity DNA chip and quantified by Qubit and qPCR. The cluster generation and sequencing were performed on the cBot and Hiseq2000 respectively, following the manufacturer’s standard cBot and sequencing protocols. The adapter sequences were removed from both ends of Solexa reads. A per script was developed to remove the low quality reads, empty tags (adaptor sequence only) and tags less than 18 nt or more than 31 nt. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer’s recommendations and index odes were added to attribute sequences to each sample. Briefly, NEB 3' SR Adaptor was directly and specifically ligated to 3' end of miRNA, siRNA and piRNA. After the 3' ligation reaction, the SR RT Primer hybridized to the excess of 3' SR Adaptor (that remained free after the 3' ligation reaction) and transformed the single-stranded DNA adaptor into a double-stranded DNA molecule. This step is important to prevent adaptor-dimer formation, besides, dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5´ SR Adaptor in the subsequent ligation step. 5´ends adapter was ligated to 5´ends of miRNAs, siRNA and piRNA. Then first strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RNase H-). PCR amplification was performed using LongAmp Taq 2X Master Mix, SR Primer for illumina and index (X) primer. PCR products were purified on a 8% polyacrylamide gel (100V, 80 min). DNA fragments corresponding to 140~160 bp (the length of small noncoding RNA plus the 3' and 5' adaptors) were recovered and dissolved in 8 μL elution buffer. At last, library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500/2000 platform and 50bp single-end reads were generated. Total RNA was isolated from cell cultures or snap-frozen tissues, in biological triplicates or greater, using TRIzol extraction and purified with the Qiagen RNA Purification Kit.

text: Sus scrofa

text: Sus scrofa

text: Sus scrofa

text: iPS-j

characteristic: title


characteristic: ENA-CHECKLIST

text: The Pirbright Institute

text: public

tag: Namespace:The Pirbright Institute

text: exposed to deltamethrin when 3-5 days old, culled 48 h post-exposure

characteristic: age

text: ArrayExpress

text: African malaria mosquito

text: biological replicate Protocols: Larvae were reared at low density in pans with de-ionized water and fed daily with ground Tetramin fish food. Adult mosquitoes were kept in the insectary conditions at 27 C and 12h light/12h dark cycle, with constant access to 10% sucrose solution. If not otherwise stated in the treatment section, the adults were kept at 80% relative humidity. Unmated 3-5 days old mosquitoes were exposed to deltamethrin using a WHO tube bioassay at a colony-specific LT10 (time required to kill approximately 10% of insects). The insecticide treatment was conducted in an insecticide testing room kept at 27 C and 80% RH. Following the exposure period mosquitoes were transferred to a maintenance insectary kept at 27C and 80% RH, with access to a 10% sucrose solution, until they were culled 2, 24, 48 or 72 hours post-exposure. Samples were ground in TRIzol and stored at -70 C until RNA isolation. Total RNA was isolated using PureLink RNA Mini Kit (Ambion) with an on-column DNase treatment step according to the manufacturer's protocol. The libraries were constructed with the PerkinElmer Sciclone NGS Workstation using the TruSeq RNA protocol (Illumina). The mRNA was isolated from 1 ug of total RNA by a poly-A+ pull-down using biotin beads, and fragmented. Following first- and second-strand synthesis, the cDNA fragments were blunt-ended, and then their 3 prime ends were adenylated to prevent formation of chimeric molecules during the adapter ligation. Then, adapters were ligated and the fragments were subjected to a bead-based size selection using Beckman Coulter XP beads (Beckman Coulter). Prior to hybridisation to the flow cell, the cDNA constructs were amplified by PCR to selectively enrich fragments with adapter molecules on both ends. The concentration of the libraries was determined by using a Qubit dsDNA HS Assay (Life Technologies) and qPCR.

text: adult

characteristic: developmental stage

text: Colonized mosquitoes derived from an eastern Ugandan population, known to have extremely high physiological resistance to pyrethroids

characteristic: environmental history

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: The Pirbright Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: exposed to deltamethrin when 3-5 days old, culled 72 h post-exposure

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen


text: The Pirbright Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: mock exposed to untreated test paper when 3-5 days old, culled 24 h post-exposure

text: ArrayExpress

characteristic: broker name

text: African malaria mosquito

characteristic: common name

text: biological replicate Protocols: Larvae were reared at low density in pans with de-ionized water and fed daily with ground Tetramin fish food. Adult mosquitoes were kept in the insectary conditions at 27 C and 12h light/12h dark cycle, with constant access to 10% sucrose solution. If not otherwise stated in the treatment section, the adults were kept at 80% relative humidity. Samples were ground in TRIzol and stored at -70 C until RNA isolation. Total RNA was isolated using PureLink RNA Mini Kit (Ambion) with an on-column DNase treatment step according to the manufacturer's protocol. The libraries were constructed with the PerkinElmer Sciclone NGS Workstation using the TruSeq RNA protocol (Illumina). The mRNA was isolated from 1 ug of total RNA by a poly-A+ pull-down using biotin beads, and fragmented. Following first- and second-strand synthesis, the cDNA fragments were blunt-ended, and then their 3 prime ends were adenylated to prevent formation of chimeric molecules during the adapter ligation. Then, adapters were ligated and the fragments were subjected to a bead-based size selection using Beckman Coulter XP beads (Beckman Coulter). Prior to hybridisation to the flow cell, the cDNA constructs were amplified by PCR to selectively enrich fragments with adapter molecules on both ends. The concentration of the libraries was determined by using a Qubit dsDNA HS Assay (Life Technologies) and qPCR.

characteristic: description

text: adult

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: The Pirbright Institute

text: public

text: exposed to deltamethrin when 3-5 days old, culled 24 h post-exposure

characteristic: age

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

characteristic: title


text: The Pirbright Institute

text: public

text: mock exposed to untreated test paper when 3-5 days old, culled 72 h post-exposure

text: ArrayExpress

text: African malaria mosquito

text: adult

characteristic: developmental stage

characteristic: environmental history

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: The Pirbright Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: blood-fed 2-3 days after eclosion and collected 24 hours after blood-feeding

text: ArrayExpress

text: African malaria mosquito

text: Protocols: Larvae were reared at low density in pans with de-ionized water and fed daily with ground Tetramin fish food. Adult mosquitoes were kept in the insectary conditions at 27 C and 12h light/12h dark cycle, with constant access to 10% sucrose solution. If not otherwise stated in the treatment section, the adults were kept at 80% relative humidity. Samples were ground in TRIzol and stored at -70 C until RNA isolation. Total RNA was isolated using PureLink RNA Mini Kit (Ambion) with an on-column DNase treatment step according to the manufacturer's protocol. The libraries were constructed with the PerkinElmer Sciclone NGS Workstation using the TruSeq RNA protocol (Illumina). The mRNA was isolated from 1 ug of total RNA by a poly-A+ pull-down using biotin beads, and fragmented. Following first- and second-strand synthesis, the cDNA fragments were blunt-ended, and then their 3 prime ends were adenylated to prevent formation of chimeric molecules during the adapter ligation. Then, adapters were ligated and the fragments were subjected to a bead-based size selection using Beckman Coulter XP beads (Beckman Coulter). Prior to hybridisation to the flow cell, the cDNA constructs were amplified by PCR to selectively enrich fragments with adapter molecules on both ends. The concentration of the libraries was determined by using a Qubit dsDNA HS Assay (Life Technologies) and qPCR.

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena

characteristic: strain


text: The Pirbright Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 24 h after eclosion

text: ArrayExpress

characteristic: broker name

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: The Pirbright Institute

text: public

text: 24 after eclosion

text: ArrayExpress

text: African malaria mosquito

characteristic: common name

characteristic: description

text: adult

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena


text: The Pirbright Institute

text: public

text: 72 h after eclosion

characteristic: age

text: ArrayExpress

text: African malaria mosquito

text: adult

characteristic: developmental stage

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex

text: fresh specimen

text: Pimperena

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: The Pirbright Institute

characteristic: INSDC center name

text: public

text: mock exposed to untreated test paper when 3-5 days old, culled 48 h post-exposure

text: ArrayExpress

text: African malaria mosquito

text: adult

characteristic: environmental history

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

characteristic: specimen with known storage state


text: The Pirbright Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blood-fed 2-3 days after eclosion and collected 72 hours after blood-feeding

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: The Pirbright Institute

text: public

characteristic: Submitter Id

text: 2 h after eclosion

text: ArrayExpress

characteristic: broker name

text: African malaria mosquito

characteristic: common name

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena


text: The Pirbright Institute

text: public

text: exposed to deltamethrin when 3-5 days old, culled 2 h post-exposure

characteristic: age

text: ArrayExpress

text: African malaria mosquito

characteristic: description

text: adult

characteristic: developmental stage

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex

text: fresh specimen


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: The Pirbright Institute

characteristic: INSDC center name

text: public

text: 48 h after eclosion

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena

characteristic: title


text: The Pirbright Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: blood-fed 2-3 days after eclosion and collected 48 h after blood-feeding

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

characteristic: specimen with known storage state

text: Pimperena


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: The Pirbright Institute

text: public

characteristic: SRA accession

text: 48 after eclosion

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena

characteristic: strain


text: The Pirbright Institute

text: public

characteristic: Submitter Id

text: mock exposed to untreated test paper when 3-5 days old, culled 2 h post-exposure

characteristic: age

text: ArrayExpress

characteristic: broker name

text: African malaria mosquito

characteristic: common name

text: adult

characteristic: environmental history

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen


characteristic: ENA-FIRST-PUBLIC

text: The Pirbright Institute

text: public

text: ArrayExpress

text: African malaria mosquito

characteristic: description

text: adult

characteristic: developmental stage

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex

text: fresh specimen


characteristic: External Id

text: The Pirbright Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: blood-fed 2-3 days after eclosion and collected 2 hours after blood-feeding

text: ArrayExpress

text: African malaria mosquito

text: adult

text: Anopheles gambiae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: fresh specimen

text: Pimperena

characteristic: title


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-TimePoint

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: EpiSC

characteristic: sample description

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Traf2 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / Traf2

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / Traf2

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / Traf2

text: Whole embryo

text: male

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Traf2 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / Traf2

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / Traf2

text: Whole embryo

text: female

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Traf2 / Traf2

text: Whole embryo

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from wild type mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from wild type mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / Traf2

text: Whole embryo

text: female

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / Traf2

text: Whole embryo

text: female

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: C57BL/6N / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from wild type mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / C57BL/6N

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / Traf2

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: female

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GGTCGTGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GAATCTGT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Traf2 / C57BL/6N

text: Whole embryo

text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / C57BL/6N

text: Whole embryo

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GAGGTGCT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


characteristic: ArrayExpress-DevelopmentalStage

text: Traf2 / C57BL/6N

text: Whole embryo

text: female

text: Mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GCATGGCT) is bases 13 to 20 of read 1 followed by CG and polyT

characteristic: sample description

text: Mus musculus


text: Traf2 / C57BL/6N

characteristic: ArrayExpress-Genotype

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GTTAGCCT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus


text: Traf2 / Traf2

text: Whole embryo

text: male

text: Mus musculus

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: 3' end enriched mRNA from mutant mouse embryo of allele Traf2tm1a(EUCOMM)Wtsi, see http://www.sanger.ac.uk/mouseportal/search?query=Traf2 for more information. This study forms part of the Deciphering the Mechanisms of Developmental Disorders (DMDD) project. A 8 base indexing sequence (GGAATGAT) is bases 13 to 20 of read 1 followed by CG and polyT

text: Mus musculus

characteristic: strain


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ZMP_phenotype_44_1_non_phenotypic_2-sc-2118763

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (ACAGTGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_1-sc-2118760

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_44_1_phenotypic_1-sc-2118761

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (CGATGTTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_2-sc-2118762

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TGACCACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

characteristic: sample description

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_3-sc-2118764

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (GCCAATGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_3-sc-2118765

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (CAGATCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_4-sc-2118766

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (ACTTGATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed

characteristic: strain


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_44_1_non_phenotypic_4-sc-2118767

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (GATCAGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_44_1_phenotypic_5-sc-2118768

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TAGCTTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_6-sc-2118769

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (GGCTACAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_5-sc-2118770

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (CTTGTACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_44_1_phenotypic_7-sc-2118771

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TGGTTGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_6-sc-2118772

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TCTCGGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

characteristic: sample description

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_8-sc-2118773

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TAAGCGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_7-sc-2118774

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TCCGTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_9-sc-2118775

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TGTACCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed

characteristic: strain


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_44_1_non_phenotypic_8-sc-2118776

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TTCTGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_44_1_non_phenotypic_9-sc-2118777

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TTGGAGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

text: ZMP_phenotype_44_1_non_phenotypic_10-sc-2118778

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TGATACGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_44_1_phenotypic_10-sc-2118779

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TGCATAGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Larval : Protruding mouth - ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_44_1_non_phenotypic_11-sc-2118780

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 44 clutch 1. A 8 base indexing sequence (TTGACTCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph44

text: Danio rerio

text: mixed


text: Mouse

text: SC

text: public

text: house mouse

text: Mus musculus

text: mRNAseq Nextera library made from RNA extracted from mutant Ba/F3 cells

characteristic: sample description

text: Mus musculus


text: Imperial College London

text: public

tag: Namespace:Imperial College London

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: RIP-Cre_LKB1_KO_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

characteristic: genetic modification

text: floxed LKB1 with RIP-Cre

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: LKB1 knockout

characteristic: phenotype

text: Mus musculus

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

characteristic: common name

text: INS1-Cre_AMPK_dKO_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK alpha 1 and 2 with Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: AMPK double knockout

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: INS1-Cre_LKB1_WT_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without Ins1-Cre

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: RIP-Cre_LKB1_KO_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

characteristic: genetic modification

text: floxed LKB1 with RIP-Cre

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: LKB1 knockout

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

text: public

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: INS1-Cre_LKB1_WT_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: wild type

characteristic: phenotype

text: Mus musculus

characteristic: title


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

characteristic: common name

text: INS1-Cre_AMPK_dKO_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK alpha 1 and 2 with Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: AMPK double knockout

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_LKB1_WT_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: strain


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: RIP-Cre_AMPK_dKO_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

characteristic: genetic modification

text: floxed AMPK alpha 1 and 2 with RIP-Cre

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

text: AMPK double knockout

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

text: public

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_LKB1_KO_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 with RIP-Cre

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: LKB1 knockout

characteristic: phenotype

text: Mus musculus


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_LKB1_WT_5 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

characteristic: common name

text: INS1-Cre_AMPK_dKO_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK alpha 1 and 2 with Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: AMPK double knockout

text: Mus musculus


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: INS1-Cre_LKB1_KO_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

characteristic: genetic modification

text: floxed LKB1 with Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: LKB1 knockout

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

text: public

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_LKB1_WT_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without RIP-Cre

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: wild type

text: Mus musculus


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_AMPK_dKO_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK alpha 1 and 2 with RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: AMPK double knockout

characteristic: phenotype

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: INS1-Cre_AMPK_WT_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK without Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: title


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: RIP-Cre_LKB1_KO_4 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

characteristic: genetic modification

text: floxed LKB1 with RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: LKB1 knockout

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

text: public

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_AMPK_WT_3 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK without RIP-Cre

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: strain


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_AMPK_WT_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: wild type

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: INS1-Cre_LKB1_KO_1 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 with Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: LKB1 knockout

characteristic: phenotype

text: Mus musculus


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: INS1-Cre_AMPK_WT_4 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

text: floxed AMPK without Ins1-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Imperial College London

text: public

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: INS1-Cre_AMPK_WT_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

characteristic: genetic modification

text: floxed AMPK without Ins1-Cre

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_AMPK_WT_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed AMPK without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 to 14 week

text: ArrayExpress

text: house mouse

text: RIP-Cre_LKB1_WT_4 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

text: gene knock out

text: floxed LKB1 without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: wild type

text: Mus musculus


text: Imperial College London

text: public

text: 12 to 14 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: RIP-Cre_LKB1_WT_2 Protocols: Pancreatic islets (50-100) were extracted from Ins1LKB1KO mice or Ins1AMPKdKO and their wild type controls aging 12-14 weeks by pancreatic distension and digestion with collagenase. Islets were incubated in RPMI media containing 11 mM glucose, 10% FCS, 100 IU/mL penicillin, 100 ug/mL streptomycin, at 5% CO2 and 37 C for 24 h. RNA was extracted using RNAeasy kit according to the manufacturer's instructions (Qiagen) All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).

characteristic: description

text: gene knock out

text: floxed LKB1 without RIP-Cre

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: wild type

characteristic: phenotype

text: Mus musculus