text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482225-sc-2013-03-07T10:02:23Z-1575225

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


status: PUBLIC

text: male

tag: attribute

text: Homo Sapien

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC_GMMAN5482228-sc-2013-03-07T10:02:26Z-1575228

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482231-sc-2013-03-07T10:02:29Z-1575231

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482234-sc-2013-03-07T10:02:32Z-1575234

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482240-sc-2013-03-07T10:02:37Z-1575240

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482243-sc-2013-03-07T10:02:40Z-1575243

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482250-sc-2013-03-07T10:02:46Z-1575250

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482256-sc-2013-03-07T10:02:52Z-1575256

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482260-sc-2013-03-07T10:02:55Z-1575260

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482266-sc-2013-03-07T10:03:02Z-1575266

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482284-sc-2013-03-07T10:03:19Z-1575284

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482290-sc-2013-03-07T10:03:25Z-1575290

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482293-sc-2013-03-07T10:03:29Z-1575293

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482299-sc-2013-03-07T10:03:34Z-1575299

text: human

text: Homo sapiens

text: Homo sapiens


E-SYBR-4:M28-4C rep2

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4C rep2

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: baker's yeast

text: Protocols: to be confirmed to be confirmed For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: filigreed colony

characteristic: morphology

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: M28-4C rep2

characteristic: title

text: trifluoroleucine resistant

characteristic: trifluoroleucine resistance

type: has member


E-SYBR-4:M28-4D rep2

characteristic: ENA-FIRST-PUBLIC

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4D rep2

text: ArrayExpress

text: baker's yeast

text: smooth colony

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4D rep2

text: trifluoroleucine sensitive


E-SYBR-4:M28-1D rep2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-1D rep2

text: ArrayExpress

text: baker's yeast

text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1D rep2

text: trifluoroleucine resistant


E-SYBR-4:M28-2A rep2

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-SYBR-4:M28-2A rep2

text: ArrayExpress

text: baker's yeast

text: filigreed colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-2A rep2

text: trifluoroleucine resistant


E-SYBR-4:M28-2B rep1

text: EMBL-EBI

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-SYBR-4:M28-2B rep1

text: ArrayExpress

text: baker's yeast

text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-2B rep1

text: trifluoroleucine resistant


E-SYBR-4:M28-1B rep2

text: EMBL-EBI

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characteristic: SRA accession

text: E-SYBR-4:M28-1B rep2

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

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text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1B rep2

text: trifluoroleucine resistant


E-SYBR-4:M28-2C rep1

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-2C rep1

text: ArrayExpress

characteristic: broker name

text: baker's yeast

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text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-2C rep1

text: trifluoroleucine sensitive


E-SYBR-4:M28-2B rep2

text: EMBL-EBI

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text: E-SYBR-4:M28-2B rep2

text: ArrayExpress

characteristic: cell line

text: baker's yeast

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text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-2B rep2

text: trifluoroleucine resistant


E-SYBR-4:M28-2A rep1

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-2A rep1

text: ArrayExpress

text: baker's yeast

text: filigreed colony

characteristic: morphology

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: M28-2A rep1

text: trifluoroleucine resistant


E-SYBR-4:M28-4D rep1

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4D rep1

text: ArrayExpress

text: baker's yeast

text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4D rep1

characteristic: title

text: trifluoroleucine sensitive

characteristic: trifluoroleucine resistance


E-SYBR-4:M28-4B rep1

characteristic: ENA-FIRST-PUBLIC

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4B rep1

text: ArrayExpress

text: baker's yeast

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characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4B rep1

text: trifluoroleucine sensitive


E-SYBR-4:M28-1B rep1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-1B rep1

text: ArrayExpress

text: baker's yeast

text: filigreed colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1B rep1

text: trifluoroleucine resistant


E-SYBR-4:M28-4A rep1

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-SYBR-4:M28-4A rep1

text: ArrayExpress

text: baker's yeast

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text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4A rep1

text: trifluoroleucine sensitive


E-SYBR-4:M28-4A rep2

text: EMBL-EBI

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text: public

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text: E-SYBR-4:M28-4A rep2

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text: baker's yeast

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text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4A rep2

text: trifluoroleucine sensitive


E-SYBR-4:M28-1C rep1

text: EMBL-EBI

text: public

characteristic: SRA accession

text: E-SYBR-4:M28-1C rep1

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: filigreed colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1C rep1

text: trifluoroleucine sensitive


E-SYBR-4:M28-4C rep1

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4C rep1

text: ArrayExpress

characteristic: broker name

text: baker's yeast

characteristic: common name

text: filigreed colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4C rep1

text: trifluoroleucine resistant


E-SYBR-4:M28-4B rep2

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-4B rep2

text: ArrayExpress

characteristic: cell line

text: baker's yeast

characteristic: description

text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-4B rep2

text: trifluoroleucine sensitive


text: Larval - day 5 ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_16_4_sibling-sc-2013-03-07T09:51:26Z-1580286

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_16_6_mutant-sc-2013-03-07T09:51:30Z-1580289

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 6. A 5 base indexing sequence (CCAAC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: male

characteristic: ArrayExpress-Sex

text: Homo Sapien

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SC_GMMAN5482162-sc-2013-03-07T10:01:11Z-1575162

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

characteristic: SRA accession

text: SC_GMMAN5482165-sc-2013-03-07T10:01:17Z-1575165

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482168-sc-2013-03-07T10:01:20Z-1575168

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482171-sc-2013-03-07T10:01:23Z-1575171

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482177-sc-2013-03-07T10:01:31Z-1575177

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482181-sc-2013-03-07T10:01:35Z-1575181

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482184-sc-2013-03-07T10:01:38Z-1575184

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482187-sc-2013-03-07T10:01:41Z-1575187

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482193-sc-2013-03-07T10:01:48Z-1575193

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482199-sc-2013-03-07T10:01:55Z-1575199

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482202-sc-2013-03-07T10:01:58Z-1575202

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482205-sc-2013-03-07T10:02:01Z-1575205

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482208-sc-2013-03-07T10:02:04Z-1575208

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482211-sc-2013-03-07T10:02:07Z-1575211

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482217-sc-2013-03-07T10:02:15Z-1575217

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482220-sc-2013-03-07T10:02:18Z-1575220

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482308-sc-2013-03-07T10:03:43Z-1575308

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482314-sc-2013-03-07T10:03:49Z-1575314

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482323-sc-2013-03-07T10:03:58Z-1575323

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482332-sc-2013-03-07T10:04:07Z-1575332

text: human

text: Homo sapiens

text: Homo sapiens


E-SYBR-4:M28-2D rep1

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-2D rep1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: baker's yeast

text: Protocols: to be confirmed to be confirmed For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: filigreed colony

characteristic: morphology

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: M28-2D rep1

characteristic: title

text: trifluoroleucine sensitive

characteristic: trifluoroleucine resistance


E-SYBR-4:M28-1A rep1

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-1A rep1

text: ArrayExpress

text: baker's yeast

text: smooth colony

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text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

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text: trifluoroleucine sensitive


E-SYBR-4:M28-1A rep2

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text: public

text: E-SYBR-4:M28-1A rep2

text: ArrayExpress

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characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1A rep2

text: trifluoroleucine sensitive


E-SYBR-4:M28-1D rep1

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characteristic: INSDC last update

text: public

text: E-SYBR-4:M28-1D rep1

text: ArrayExpress

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text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1D rep1

text: trifluoroleucine resistant


E-SYBR-4:M28-2C rep2

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-SYBR-4:M28-2C rep2

text: ArrayExpress

text: baker's yeast

text: smooth colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-2C rep2

text: trifluoroleucine sensitive


E-SYBR-4:M28-1C rep2

text: EMBL-EBI

text: public

text: E-SYBR-4:M28-1C rep2

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: filigreed colony

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: M28-1C rep2

text: trifluoroleucine sensitive


text: The Babraham Institute

text: public

tag: Namespace:The Babraham Institute

text: 7 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mice were kept until 6-8 weeks of age To isolate activated, proliferating satellite cells from regenerating muscle, the right and left gastrocnemius of knockout and control mice (4 mice per genotype) was injected with 50 ul of 10 uM cardiotoxin. 50 micrograms cardiotoxin was administered to each animal.

text: adult

characteristic: developmental stage

text: wild type

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: muscle

characteristic: organism part

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Babraham Institute

text: public

text: 7 week

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: gene knock out

characteristic: genetic modification

text: p38 -/-

text: Mus musculus

text: Mus musculus

text: muscle

text: Mus musculus

text: fresh specimen

characteristic: title


text: macrophages

characteristic: ArrayExpress-CellType

text: Wildtype

characteristic: ArrayExpress-Genotype

text: mus musculus

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E14_iCreiFlp_ESC_derived_macrophage_uninfected_2-sc-2013-03-11T09:26:13Z-1581606

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from macrophages derived from murine embryonic stem cells

characteristic: sample description

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E14_iCreiFlp_ESC_derived_macrophage_infected_1-sc-2013-03-11T09:26:17Z-1581609

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: RNA from macrophages derived from murine embryonic stem cells infected with Salmonella Typhimurium

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: E14_iCreiFlp_ESC_derived_macrophage_infected_3-sc-2013-03-11T09:26:19Z-1581611

text: house mouse

text: Mus musculus

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

text: E14_iCreiFlp_ESC_derived_macrophage_infected_4-sc-2013-03-11T09:26:20Z-1581612

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

characteristic: ArrayExpress-Sex

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482223-sc-2013-03-07T10:02:21Z-1575223

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482229-sc-2013-03-07T10:02:27Z-1575229

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482232-sc-2013-03-07T10:02:30Z-1575232

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482235-sc-2013-03-07T10:02:32Z-1575235

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482241-sc-2013-03-07T10:02:38Z-1575241

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482244-sc-2013-03-07T10:02:41Z-1575244

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482247-sc-2013-03-07T10:02:44Z-1575247

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482251-sc-2013-03-07T10:02:47Z-1575251

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482258-sc-2013-03-07T10:02:53Z-1575258

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482276-sc-2013-03-07T10:03:11Z-1575276

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482282-sc-2013-03-07T10:03:17Z-1575282

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482285-sc-2013-03-07T10:03:20Z-1575285

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482291-sc-2013-03-07T10:03:27Z-1575291

text: human

text: Homo sapiens

text: Homo sapiens


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

text: 129_mice_bone_marrow_derived_macrophage_uninfected_3-sc-2013-03-11T09:26:24Z-1581615

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from bone marrow of 129 mice

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

text: 129_mice_bone_marrow_derived_macrophage_infected_2-sc-2013-03-11T09:26:28Z-1581618

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from bone marrow of 129 mice infected with Salmonella Typhimurium

text: Mus musculus


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482300-sc-2013-03-07T10:03:35Z-1575300

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482306-sc-2013-03-07T10:03:41Z-1575306

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482309-sc-2013-03-07T10:03:44Z-1575309

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482315-sc-2013-03-07T10:03:50Z-1575315

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482324-sc-2013-03-07T10:03:59Z-1575324

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482340-sc-2013-03-07T10:04:13Z-1575340

text: human

text: Homo sapiens

text: Homo sapiens


text: EMBL-EBI

text: public

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: to be confirmed to be confirmed For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: allergic bronchopulmonary aspergillosis

characteristic: disease state

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: healthy

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: baker's yeast

text: Protocols: Fungal cell preparation: 5 S. cerevisiae strains were grown at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid YPD medium (1%, w/v yeast extract, 2%, w/v peptone and 2% w/v glucose). 5 C. albicans and parapsilosis strains were grown at at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid Saboraud medium (1%, w/v peptone and 2% w/v glucose). Cells were then harvested by centrifugation, washed twice with sterile water and resuspended in fresh RPMI 1640 complete medium. 4 A. fumigatus strains were grown at 37degC in solid Saboraud medium. Spores were then collected using PBS + 0.1% Tween and then washed twice with sterile water and resuspended in fresh RPMI 1640 complete. Human cell preparation: PBMCs were isolated from buffy coat blood sample from 6 healthy donors from the Transfusion Unit of the Careggi Hospital (Florence, Italy) by Ficoll-Hypaque density gradient centrifugation (Biochrom AG). Monocytes were isolated from low density PBMCs by magnetic enrichment with anti-CD14 beads (Miltenyi Biotec). Cells were cultured in RPMI complete medium (RPMI 1640, 10% w/v FBS, 1% w/v 200 mM glutamine, 1% w/v 50 mM sodium pyruvate, penicillin-streptomicin) in the presence of GM-CSF (800 U/ml) and recombinant IL-4 (1000 U/ml) for 6 days to allow dendritic cell (DC) differentiation (37degC, 5% CO2). Cell treatment: DCs were cultured for 4 hours with the different fungal strains at a stimuli:DC ratio of 5:1 (time= 4hr, 37degC, 5% CO2, complete RPMI medium). As controls, DCs or the single fungal strain were grown alone in the same experimental condition. RNA was extracted from cells using the standard TriZol protocol. For A. fumigatus containing samples, prior to TriZol extraction, fungal cells were disrupted by grinding in liquid nitrogen with pestle and mortar. RNA samples were then quantified, and their integrity checked using NanoChip BioAnalyzer technology (Agilent). For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line


E-SYBR-2:DC DC6704 + SK1

text: EMBL-EBI

text: public

characteristic: INSDC status

text: E-SYBR-2:DC DC6704 + SK1

text: ArrayExpress

text: baker's yeast

text: DC DC6704

text: Homo sapiens + Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC6704 + SK1


text: female

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: SRA accession

text: SC_DSCC15506260-sc-2013-03-21T10:41:16Z-1600182

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Tumour

characteristic: sample description

text: Mus musculus

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: asthma

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: chronic cavitary pulmonary aspergillosis

characteristic: disease state

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


E-SYBR-2:DC DC6704 untreated

text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-SYBR-2:DC DC6704 untreated

text: ArrayExpress

text: human

text: DC DC6704

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: DC DC6704 untreated


E-SYBR-2:DC DC5412 + Y13EU

text: EMBL-EBI

text: public

characteristic: SRA accession

text: E-SYBR-2:DC DC5412 + Y13EU

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: DC DC5412

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: DC DC5412 + Y13EU


E-SYBR-2:DC DC5618 + Y13EU

characteristic: ENA-FIRST-PUBLIC

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5618 + Y13EU

text: ArrayExpress

text: baker's yeast

text: DC DC5618

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5618 + Y13EU


E-SYBR-2:DC DC5618 + BB1533

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5618 + BB1533

text: ArrayExpress

text: baker's yeast

text: DC DC5618

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5618 + BB1533


text: male

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: SC_GMFUL5306378_LQ-sc-2013-03-15T13:26:11Z-1584579

text: human

text: Homo sapiens

text: Low input test

characteristic: sample description

text: Homo sapiens


text: Glia

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

text: public

text: m_5hmC_Control-sc-2013-03-19T13:56:12Z-1599118

text: house mouse

characteristic: common name

text: Mus musculus

text: gDNA from FACS sorted glia

text: Mus musculus

characteristic: strain


text: Mouse

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC_ALLPax55490709-sc-2013-03-21T15:01:12Z-1584610

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mouse

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_ALLPax55490712-sc-2013-03-21T15:01:16Z-1584613

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490715-sc-2013-03-21T15:01:19Z-1584616

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490718-sc-2013-03-21T15:01:23Z-1584619

text: house mouse

text: Mus musculus

text: Mus musculus


E-SYBR-2:DC DC5412+ BB1533

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5412+ BB1533

text: ArrayExpress

characteristic: broker name

text: baker's yeast

characteristic: description

text: DC DC5412

characteristic: individual

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: DC DC5412+ BB1533

characteristic: title


E-SYBR-2:DC DC5412 + YB7

characteristic: External Id

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5412 + YB7

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: DC DC5412

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5412 + YB7


text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

text: public

text: m_5hmC_Neuron_2-sc-2013-03-19T13:56:16Z-1599121

text: house mouse

text: Mus musculus

text: gDNA from FACS sorted neurons

characteristic: sample description

text: Mus musculus

characteristic: strain


text: Mouse

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC_ALLPax55490721-sc-2013-03-21T15:01:28Z-1584622

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mouse

characteristic: External Id

text: SC

text: public

text: SC_ALLPax55490724-sc-2013-03-21T15:01:32Z-1584625

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490730-sc-2013-03-21T15:01:39Z-1584631

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490733-sc-2013-03-21T15:01:42Z-1584634

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490736-sc-2013-03-21T15:01:45Z-1584637

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_ALLPax55490739-sc-2013-03-21T15:01:49Z-1584640

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508562-sc-2013-03-22T14:11:02Z-1602518

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508565-sc-2013-03-22T14:11:09Z-1602521

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508568-sc-2013-03-22T14:11:12Z-1602524

text: house mouse

text: Mus musculus

text: Mus musculus


text: MOUSE

text: SC

text: public

text: SC_GCIM5508571-sc-2013-03-22T14:11:16Z-1602527

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mouse

text: SC

text: public

text: SC_DSCC15487737-sc-2013-03-12T07:41:35Z-1581365

text: house mouse

text: Mus musculus

text: Tail

text: Mus musculus


text: male

text: Mouse

text: SC

text: public

text: SC_DSCC15487740-sc-2013-03-12T07:41:39Z-1581368

text: house mouse

text: Mus musculus

text: Tail

text: Mus musculus


text: Wildtype

characteristic: ArrayExpress-Genotype

text: VNO

characteristic: ArrayExpress-OrganismPart

text: Wildtype

characteristic: ArrayExpress-Phenotype

text: mixed

text: Mouse

text: SC

text: public

text: newbornVNO1-sc-2013-03-14T09:46:39Z-1583318

text: house mouse

text: Mus musculus

text: RNA from VNO

text: Mus musculus


E-SYBR-2:DC DC5412 untreated

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: E-SYBR-2:DC DC5412 untreated

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: Fungal cell preparation: 5 S. cerevisiae strains were grown at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid YPD medium (1%, w/v yeast extract, 2%, w/v peptone and 2% w/v glucose). 5 C. albicans and parapsilosis strains were grown at at 28degC with stirring (150 r.p.m.) until mid-exponential phase in liquid Saboraud medium (1%, w/v peptone and 2% w/v glucose). Cells were then harvested by centrifugation, washed twice with sterile water and resuspended in fresh RPMI 1640 complete medium. 4 A. fumigatus strains were grown at 37degC in solid Saboraud medium. Spores were then collected using PBS + 0.1% Tween and then washed twice with sterile water and resuspended in fresh RPMI 1640 complete. Human cell preparation: PBMCs were isolated from buffy coat blood sample from 6 healthy donors from the Transfusion Unit of the Careggi Hospital (Florence, Italy) by Ficoll-Hypaque density gradient centrifugation (Biochrom AG). Monocytes were isolated from low density PBMCs by magnetic enrichment with anti-CD14 beads (Miltenyi Biotec). Cells were cultured in RPMI complete medium (RPMI 1640, 10% w/v FBS, 1% w/v 200 mM glutamine, 1% w/v 50 mM sodium pyruvate, penicillin-streptomicin) in the presence of GM-CSF (800 U/ml) and recombinant IL-4 (1000 U/ml) for 6 days to allow dendritic cell (DC) differentiation (37degC, 5% CO2). Cell treatment: DCs were cultured for 4 hours with the different fungal strains at a stimuli:DC ratio of 5:1 (time= 4hr, 37degC, 5% CO2, complete RPMI medium). As controls, DCs or the single fungal strain were grown alone in the same experimental condition. RNA was extracted from cells using the standard TriZol protocol. For A. fumigatus containing samples, prior to TriZol extraction, fungal cells were disrupted by grinding in liquid nitrogen with pestle and mortar. RNA samples were then quantified, and their integrity checked using NanoChip BioAnalyzer technology (Agilent). For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).

characteristic: description

text: DC DC5412

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: DC DC5412 untreated

characteristic: title


E-SYBR-2:DC DC6704 + Y13EU

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC6704 + Y13EU

text: ArrayExpress

text: baker's yeast

text: DC DC6704

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: DC DC6704 + Y13EU


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Wildtype

text: VNO

text: Wildtype

text: mixed

characteristic: ArrayExpress-Sex

text: Mouse

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: SRA accession

text: newbornVNO2-sc-2013-03-14T09:46:44Z-1583319

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from VNO

characteristic: sample description

text: Mus musculus

characteristic: strain


E-SYBR-2:YB7 rep2

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: E-SYBR-2:YB7 rep2

text: ArrayExpress

characteristic: broker name

text: baker's yeast

characteristic: common name

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: YB7 rep2


E-SYBR-2:SK1 rep2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

text: E-SYBR-2:SK1 rep2

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain or line

text: SK1 rep2

characteristic: title


E-SYBR-2:DC DC5412 + SK1

text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-SYBR-2:DC DC5412 + SK1

text: ArrayExpress

text: baker's yeast

text: DC DC5412

characteristic: individual

text: Homo sapiens + Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5412 + SK1


E-SYBR-2:DC 5618

text: EMBL-EBI

text: public

characteristic: SRA accession

text: E-SYBR-2:DC 5618

characteristic: Submitter Id

text: ArrayExpress

text: human

text: DC DC5618

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: DC 5618


E-SYBR-2:DC DC5618 + SK1

text: EMBL-EBI

text: public

text: E-SYBR-2:DC DC5618 + SK1

text: ArrayExpress

text: baker's yeast

text: DC DC5618

text: Homo sapiens + Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: DC DC5618 + SK1


E-SYBR-2:YA5 rep2

text: EMBL-EBI

text: public

text: E-SYBR-2:YA5 rep2

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: YA5 rep2


text: macrophages

characteristic: ArrayExpress-CellType

text: Wildtype

characteristic: ArrayExpress-Genotype

text: mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: 129_mice_bone_marrow_derived_macrophage_uninfected_1-sc-2013-03-11T09:26:22Z-1581613

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from bone marrow of 129 mice

characteristic: sample description

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: 129_mice_bone_marrow_derived_macrophage_uninfected_4-sc-2013-03-11T09:26:26Z-1581616

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

text: ArrayExpress

characteristic: broker name

text: Embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: Oct4-GIP mouse embryonic stem (ES) cells (Ying et al. 2002) were cultured in either standard ES cell medium (GMEM with 10% fetal calf serum (Biosera), 0.1mM non-essential amino acids, 2mM L-Glutamine, 1mM sodium pyruvate, 0.1mM beta-mercaptoethanol and 106 units/L LIF (ESGRO, Millipore)), or in 2i/LIF medium (GMEM with 10% Knock-Out Serum Replacement (Life Technologies), 1% fetal calf serum (Biosera or Sigma), 0.1mM non-essential amino acids, 2mM L-Glutamine, sodium pyruvate, 0.1mM beta-mercaptoethanol, 1?M PD0325901 (AxonMedChem), 3?M CHIR99021 (AxonMedChem) and 106 units/L LIF (ESGRO, Millipore)). 1?g/ml puromycin was added to Oct4-GIP ES cell cultures during expansion. Cells were transfected with 1000 pmol antisense oligonucleotides (Integrated DNA Technologies) using the Mouse ES Cell Nuclefector Kit (Lonza). Total RNA was isolated using miRNeasy extraction kit (Qiagen) with in-column DNase treatment. Total RNA was depleted from ribosomal RNA with the Low Input Ribo-Zero rRNA removal system (Epicentre). rRNA-depleted samples were then fragmented with RNA fragmentation reagent (Ambion), purified using the RNeasy MinElute Kit (Qiagen) and treated with alkaline phosphatase (New England Biolabs) for 30 minutes at 37?C. 5' dephosphorylated RNA was treated with T4 PNK Kinase (New England Biolabs) for 60 minutes at 37?C. The resulting RNA (5' mono-phosphorylated and 3? OH) was purified with RNeasy MinElute (Qiagen) and ligated with RNA 3? and 5' TruSeq adapters (Illumina). Indexes 1-6 were used for PCR amplification.

characteristic: description

text: Mus musculus

text: Mus musculus

text: normal

characteristic: phenotype

text: Mus musculus

characteristic: strain


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: Embryonic stem cell

text: house mouse

text: Protocols: Oct4-GIP mouse embryonic stem (ES) cells (Ying et al. 2002) were cultured in either standard ES cell medium (GMEM with 10% fetal calf serum (Biosera), 0.1mM non-essential amino acids, 2mM L-Glutamine, 1mM sodium pyruvate, 0.1mM beta-mercaptoethanol and 106 units/L LIF (ESGRO, Millipore)), or in 2i/LIF medium (GMEM with 10% Knock-Out Serum Replacement (Life Technologies), 1% fetal calf serum (Biosera or Sigma), 0.1mM non-essential amino acids, 2mM L-Glutamine, sodium pyruvate, 0.1mM beta-mercaptoethanol, 1?M PD0325901 (AxonMedChem), 3?M CHIR99021 (AxonMedChem) and 106 units/L LIF (ESGRO, Millipore)). 1?g/ml puromycin was added to Oct4-GIP ES cell cultures during expansion. Total RNA was isolated using miRNeasy extraction kit (Qiagen) with in-column DNase treatment. Total RNA was depleted from ribosomal RNA with the Low Input Ribo-Zero rRNA removal system (Epicentre). rRNA-depleted samples were then fragmented with RNA fragmentation reagent (Ambion), purified using the RNeasy MinElute Kit (Qiagen) and treated with alkaline phosphatase (New England Biolabs) for 30 minutes at 37?C. 5' dephosphorylated RNA was treated with T4 PNK Kinase (New England Biolabs) for 60 minutes at 37?C. The resulting RNA (5' mono-phosphorylated and 3? OH) was purified with RNeasy MinElute (Qiagen) and ligated with RNA 3? and 5' TruSeq adapters (Illumina). Indexes 1-6 were used for PCR amplification.

text: Mus musculus

text: Mus musculus

text: normal

text: Mus musculus

characteristic: title


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: MiliDAH and Miwi2DAH mice were crossed with Oct4/GFP trangenic mice to label germ cells with eGFP. To isolate E16.5 and postnatal day 7 germ cells, single cell suspensions of testis were obtained by two step enzymatic digestion and GFP-positive FACS-sorted. Mili and Miwi2 RNPs were immunoprecipitated as described in Reuter, M. et al. (2009). Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform. Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform.

text: Mili_Ctl, Mili_C-DAH

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: testis

characteristic: organism part

text: Mus musculus


characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: testis

text: Mus musculus


text: Epiblast

characteristic: ArrayExpress-CellType

characteristic: ArrayExpress-DevelopmentalStage

text: 3a-/-; 3b-/-

characteristic: ArrayExpress-Genotype

text: Epiblast

characteristic: ArrayExpress-OrganismPart

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: E7_5_Epi_DKO_1-sc-2013-03-18T14:51:23Z-1584801

text: house mouse

text: Mus musculus

text: RNA from E7.5 epiblast

characteristic: sample description

text: Mus musculus


text: EPC

text: 3a+/+; 3b+/+

text: EPC

text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E7_5_EPC_Ctrl_1-sc-2013-03-18T14:51:26Z-1584804

text: house mouse

text: Mus musculus

text: RNA from E7.5 EPC

text: Mus musculus


text: EPC

text: 3a+/-; 3b+/-

text: EPC

text: Mus Musculus

text: SC

text: public

text: E7_5_EPC_Het_1-sc-2013-03-18T14:51:29Z-1584807

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from E7.5 EPC

text: Mus musculus


text: OB+OC

text: Wildtype

text: Bone

text: Various

characteristic: ArrayExpress-Phenotype

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: 6 weeks

characteristic: ArrayExpress-TimePoint

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA from Bone

text: Mus musculus

characteristic: strain


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

text: ZMP_phenotype_13_4_sibling-sc-2013-03-25T15:42:24Z-1603265

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 13 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph13

text: Danio rerio

text: mixed


text: Larval: protruding mouth: ZFS:0000035 - Larval: Day 4:ZFS:0000036

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_14_2_mutant-sc-2013-03-25T17:02:32Z-1603275

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

characteristic: sample description

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_14_3_sibling-sc-2013-03-25T17:02:35Z-1603278

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_14_5_mutant-sc-2013-03-25T17:02:38Z-1603281

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 5. A 5 base indexing sequence (AACCG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_14_6_sibling-sc-2013-03-25T17:02:42Z-1603284

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 6. A 5 base indexing sequence (AGCGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_15_2_mutant-sc-2013-03-25T17:07:40Z-1603287

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 15 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_15_3_sibling-sc-2013-03-25T17:07:44Z-1603290

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 15 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

text: Danio rerio

text: mixed

characteristic: strain


text: macrophages

characteristic: ArrayExpress-CellType

text: Wildtype

characteristic: ArrayExpress-Genotype

text: mus musculus

text: SC

text: public

text: 129_mice_bone_marrow_derived_macrophage_uninfected_2-sc-2013-03-11T09:26:23Z-1581614

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from bone marrow of 129 mice

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

characteristic: INSDC center name

text: public

text: 129_mice_bone_marrow_derived_macrophage_infected_1-sc-2013-03-11T09:26:27Z-1581617

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from bone marrow of 129 mice infected with Salmonella Typhimurium

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

text: 129_mice_bone_marrow_derived_macrophage_infected_4-sc-2013-03-11T09:26:30Z-1581620

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Hatching : 52 hpf Long pec ZFS:0000033 - pec fin ZFS:0000034

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_10_1_sibling-sc-2013-03-07T09:41:20Z-1580258

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype10 clutch1. A 5 base indexing sequence(AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph10

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_10_3_mutant-sc-2013-03-07T09:41:24Z-1580261

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 10 clutch3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph10

characteristic: sample description

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_11_1_mutant-sc-2013-03-07T09:46:17Z-1580263

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 11 clutch1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_11_2_sibling-sc-2013-03-07T09:46:22Z-1580266

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 11 clutch2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed


text: Larval : Day 5 - ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_11_4_mutant-sc-2013-03-07T09:46:26Z-1580269

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotypen 11 clutch4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph11

text: Danio rerio

text: mixed

characteristic: strain


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

text: ZMP_phenotype12_1_sibling-sc-2013-03-07T09:46:29Z-1580272

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal sibling embryos from ZMP phenotype 12 clutch1. A 5 base indexing sequence(AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - protruding mouth ZFS:0000035

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_12_3_mutant-sc-2013-03-07T09:46:35Z-1580275

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 12 clutch3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph12

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_16_2_mutant-sc-2013-03-07T09:51:18Z-1580281

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Epiblast

characteristic: ArrayExpress-CellType

text: 3a+/+; 3b+/+

characteristic: ArrayExpress-Genotype

text: Epiblast

characteristic: ArrayExpress-OrganismPart

text: Mus Musculus

text: SC

text: public

text: E7_5_Epi_Ctrl_2-sc-2013-03-18T14:51:22Z-1584800

text: house mouse

text: Mus musculus

text: RNA from E7.5 epiblast

text: Mus musculus


text: Epiblast

text: 3a+/-; 3b+/-

text: Epiblast

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: E7_5_Epi_Het_1-sc-2013-03-18T14:51:25Z-1584803

text: house mouse

text: Mus musculus

text: RNA from E7.5 epiblast

text: Mus musculus


text: EPC

text: 3a+/-; 3b-/-

text: EPC

text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E7_5_EPC_3bKO_1-sc-2013-03-18T14:51:28Z-1584806

text: house mouse

text: Mus musculus

text: RNA from E7.5 EPC

characteristic: sample description

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E14_iCreiFlp_ESC_derived_macrophage_uninfected_3-sc-2013-03-11T09:26:15Z-1581607

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: RNA from macrophages derived from murine embryonic stem cells

text: Mus musculus


text: macrophages

text: Wildtype

text: mus musculus

characteristic: External Id

text: SC

text: public

text: E14_iCreiFlp_ESC_derived_macrophage_infected_2-sc-2013-03-11T09:26:18Z-1581610

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from macrophages derived from murine embryonic stem cells infected with Salmonella Typhimurium

text: Mus musculus


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

text: public

text: ZMP_phenotype_16_3_sibling-sc-2013-03-07T09:51:24Z-1580284

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed

characteristic: strain


text: Larval - day 5 ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_16_5_mutant-sc-2013-03-07T09:51:27Z-1580287

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 16 clutch 5. A 5 base indexing sequence (AACCG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: Larval - day 5 ZFS:0000037

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_16_6_sibling-sc-2013-03-07T09:51:31Z-1580290

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 16 clutch 6. A 5 base indexing sequence (AGCGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph16

text: Danio rerio

text: mixed


text: male

characteristic: ArrayExpress-Sex

text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: public

text: SC_GMMAN5482163-sc-2013-03-07T10:01:14Z-1575163

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_GMMAN5482169-sc-2013-03-07T10:01:21Z-1575169

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SC_GMMAN5482172-sc-2013-03-07T10:01:25Z-1575172

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

characteristic: INSDC status

text: SC_GMMAN5482178-sc-2013-03-07T10:01:32Z-1575178

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

characteristic: SRA accession

text: SC_GMMAN5482185-sc-2013-03-07T10:01:39Z-1575185

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482191-sc-2013-03-07T10:01:46Z-1575191

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

characteristic: External Id

text: SC

text: public

text: SC_GMMAN5482194-sc-2013-03-07T10:01:49Z-1575194

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482197-sc-2013-03-07T10:01:52Z-1575197

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482200-sc-2013-03-07T10:01:56Z-1575200

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482203-sc-2013-03-07T10:01:59Z-1575203

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482209-sc-2013-03-07T10:02:05Z-1575209

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482212-sc-2013-03-07T10:02:08Z-1575212

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482221-sc-2013-03-07T10:02:19Z-1575221

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482224-sc-2013-03-07T10:02:21Z-1575224

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482227-sc-2013-03-07T10:02:25Z-1575227

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482233-sc-2013-03-07T10:02:31Z-1575233

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482236-sc-2013-03-07T10:02:33Z-1575236

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482239-sc-2013-03-07T10:02:36Z-1575239

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482242-sc-2013-03-07T10:02:39Z-1575242

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482248-sc-2013-03-07T10:02:45Z-1575248

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482252-sc-2013-03-07T10:02:48Z-1575252

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482255-sc-2013-03-07T10:02:51Z-1575255

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482268-sc-2013-03-07T10:03:04Z-1575268

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482274-sc-2013-03-07T10:03:09Z-1575274

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482283-sc-2013-03-07T10:03:18Z-1575283

text: human

text: Homo sapiens

text: Homo sapiens


text: female

text: Mus Musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: SC_DSCC15506259-sc-2013-03-21T10:41:14Z-1600181

text: house mouse

text: Mus musculus

text: Tumour

characteristic: sample description

text: Mus musculus


text: female

text: Homo Sapien

text: SC

characteristic: INSDC center name

text: public

text: SC_GMMAN5482292-sc-2013-03-07T10:03:28Z-1575292

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482298-sc-2013-03-07T10:03:33Z-1575298

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482301-sc-2013-03-07T10:03:36Z-1575301

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482307-sc-2013-03-07T10:03:42Z-1575307

text: human

text: Homo sapiens

text: Homo sapiens


text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482316-sc-2013-03-07T10:03:51Z-1575316

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482322-sc-2013-03-07T10:03:57Z-1575322

text: human

text: Homo sapiens

text: Homo sapiens


text: male

text: Homo Sapien

text: SC

text: public

text: SC_GMMAN5482348-sc-2013-03-07T10:04:20Z-1575348

text: human

text: Homo sapiens

text: Homo sapiens


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_13_2_mutant-sc-2013-03-25T15:42:19Z-1603260

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 13 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph13

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_13_3_sibling-sc-2013-03-25T15:42:22Z-1603263

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 13 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph13

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

text: SC

text: public

text: ZMP_phenotype_13_4_mutant-sc-2013-03-25T15:42:23Z-1603264

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 13 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph13

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_13_5_sibling-sc-2013-03-25T15:42:26Z-1603267

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 13 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph13

text: Danio rerio

text: mixed


text: Larval: protruding mouth: ZFS:0000035 - Larval: Day 4:ZFS:0000036

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_14_1_mutant-sc-2013-03-25T17:02:28Z-1603273

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: ZMP_phenotype_14_1_sibling-sc-2013-03-25T17:02:30Z-1603274

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_14_2_sibling-sc-2013-03-25T17:02:33Z-1603276

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: ZMP_phenotype_14_3_mutant-sc-2013-03-25T17:02:34Z-1603277

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_14_4_mutant-sc-2013-03-25T17:02:36Z-1603279

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_14_4_sibling-sc-2013-03-25T17:02:37Z-1603280

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_14_5_sibling-sc-2013-03-25T17:02:39Z-1603282

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_14_6_mutant-sc-2013-03-25T17:02:41Z-1603283

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 6. A 5 base indexing sequence (CCAAC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_15_1_mutant-sc-2013-03-25T17:07:36Z-1603285

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 15 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_15_1_sibling-sc-2013-03-25T17:07:38Z-1603286

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 15 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

characteristic: sample description

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

text: ZMP_phenotype_15_2_sibling-sc-2013-03-25T17:07:42Z-1603288

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 15 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

text: Danio rerio

text: mixed


text: Larval: Day 5 ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_15_3_mutant-sc-2013-03-25T17:07:43Z-1603289

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 15 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph15

text: Danio rerio

text: mixed


text: female

characteristic: ArrayExpress-Sex

text: Mouse

text: SC

text: public

text: SC_DSCC15487736-sc-2013-03-12T07:41:34Z-1581364

text: house mouse

text: Mus musculus

text: Tail

text: Mus musculus

characteristic: strain


text: female

text: Mouse

text: SC

text: public

text: SC_DSCC15487739-sc-2013-03-12T07:41:38Z-1581367

text: house mouse

characteristic: common name

text: Mus musculus

text: Tail

text: Mus musculus


text: male

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: SC_GMFUL5306362_LQ-sc-2013-03-15T13:26:07Z-1584577

text: human

text: Homo sapiens

text: Low input test

text: Homo sapiens


text: Glia

characteristic: ArrayExpress-CellType

text: Adult

text: Wildtype

characteristic: ArrayExpress-Genotype

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: m_5mC_Glia_2-sc-2013-03-19T13:56:11Z-1599117

text: house mouse

text: Mus musculus

text: gDNA from FACS sorted glia

text: Mus musculus


text: Neurons

text: Adult

text: Wildtype

text: Brain

text: wildtype

text: male

text: Mus musculus

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: m_5hmC_Neuron_1-sc-2013-03-19T13:56:15Z-1599120

text: house mouse

text: Mus musculus

text: gDNA from FACS sorted neurons

text: Mus musculus


text: Glia

text: Adult

text: Wildtype

text: Brain

text: wildtype

text: male

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

characteristic: External Id

text: SC

text: public

text: m_5hmC_Glia_2-sc-2013-03-19T13:56:18Z-1599123

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: gDNA from FACS sorted glia

characteristic: sample description

text: Mus musculus


text: Wildtype

text: MOE

text: Wildtype

text: mixed

characteristic: ArrayExpress-Sex

text: Mouse

text: SC

characteristic: INSDC center name

text: public

text: newbornMOE1-sc-2013-03-14T09:46:47Z-1583321

text: house mouse

characteristic: common name

text: Mus musculus

text: RNA from MOE

text: Mus musculus

characteristic: strain


text: Wildtype

characteristic: ArrayExpress-Genotype

text: MOE

characteristic: ArrayExpress-OrganismPart

text: Wildtype

text: mixed

text: Mouse

characteristic: ArrayExpress-Species

text: SC

text: public

text: newbornMOE2-sc-2013-03-14T09:46:48Z-1583322

text: house mouse

text: Mus musculus

text: RNA from MOE

text: Mus musculus


text: NAAS

text: public

text: Japanese rice

text: Whole genome resequencing of the Korean rice, SM, using Illumina Hiseq 2000

characteristic: description

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: Embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: Oct4-GIP mouse embryonic stem (ES) cells (Ying et al. 2002) were cultured in either standard ES cell medium (GMEM with 10% fetal calf serum (Biosera), 0.1mM non-essential amino acids, 2mM L-Glutamine, 1mM sodium pyruvate, 0.1mM beta-mercaptoethanol and 106 units/L LIF (ESGRO, Millipore)), or in 2i/LIF medium (GMEM with 10% Knock-Out Serum Replacement (Life Technologies), 1% fetal calf serum (Biosera or Sigma), 0.1mM non-essential amino acids, 2mM L-Glutamine, sodium pyruvate, 0.1mM beta-mercaptoethanol, 1?M PD0325901 (AxonMedChem), 3?M CHIR99021 (AxonMedChem) and 106 units/L LIF (ESGRO, Millipore)). 1?g/ml puromycin was added to Oct4-GIP ES cell cultures during expansion. Cells were transfected with 1000 pmol antisense oligonucleotides (Integrated DNA Technologies) using the Mouse ES Cell Nuclefector Kit (Lonza). Total RNA was isolated using miRNeasy extraction kit (Qiagen) with in-column DNase treatment. Total RNA was depleted from ribosomal RNA with the Low Input Ribo-Zero rRNA removal system (Epicentre). rRNA-depleted samples were then fragmented with RNA fragmentation reagent (Ambion), purified using the RNeasy MinElute Kit (Qiagen) and treated with alkaline phosphatase (New England Biolabs) for 30 minutes at 37?C. 5' dephosphorylated RNA was treated with T4 PNK Kinase (New England Biolabs) for 60 minutes at 37?C. The resulting RNA (5' mono-phosphorylated and 3? OH) was purified with RNeasy MinElute (Qiagen) and ligated with RNA 3? and 5' TruSeq adapters (Illumina). Indexes 1-6 were used for PCR amplification.

text: Mus musculus

text: Mus musculus

text: normal

characteristic: phenotype

text: Mus musculus

characteristic: title


characteristic: External Id

text: EMBL-EBI

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Embryonic stem cell

text: house mouse

text: Protocols: Oct4-GIP mouse embryonic stem (ES) cells (Ying et al. 2002) were cultured in either standard ES cell medium (GMEM with 10% fetal calf serum (Biosera), 0.1mM non-essential amino acids, 2mM L-Glutamine, 1mM sodium pyruvate, 0.1mM beta-mercaptoethanol and 106 units/L LIF (ESGRO, Millipore)), or in 2i/LIF medium (GMEM with 10% Knock-Out Serum Replacement (Life Technologies), 1% fetal calf serum (Biosera or Sigma), 0.1mM non-essential amino acids, 2mM L-Glutamine, sodium pyruvate, 0.1mM beta-mercaptoethanol, 1?M PD0325901 (AxonMedChem), 3?M CHIR99021 (AxonMedChem) and 106 units/L LIF (ESGRO, Millipore)). 1?g/ml puromycin was added to Oct4-GIP ES cell cultures during expansion. Total RNA was isolated using miRNeasy extraction kit (Qiagen) with in-column DNase treatment. Total RNA was depleted from ribosomal RNA with the Low Input Ribo-Zero rRNA removal system (Epicentre). rRNA-depleted samples were then fragmented with RNA fragmentation reagent (Ambion), purified using the RNeasy MinElute Kit (Qiagen) and treated with alkaline phosphatase (New England Biolabs) for 30 minutes at 37?C. 5' dephosphorylated RNA was treated with T4 PNK Kinase (New England Biolabs) for 60 minutes at 37?C. The resulting RNA (5' mono-phosphorylated and 3? OH) was purified with RNeasy MinElute (Qiagen) and ligated with RNA 3? and 5' TruSeq adapters (Illumina). Indexes 1-6 were used for PCR amplification.

text: Mus musculus

characteristic: organism

text: Mus musculus

text: normal

text: Mus musculus


text: Human

text: Promega

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:23Z-1607081

text: human

characteristic: common name

text: Homo sapiens

text: genomic DNA

characteristic: sample description

text: Homo sapiens

text: Promega

characteristic: strain


text: Human

characteristic: ArrayExpress-Species

text: Promega

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:26Z-1607084

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

characteristic: INSDC first public

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:29Z-1607087

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:32Z-1607090

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:35Z-1607093

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:38Z-1607096

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:41Z-1607099

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:44Z-1607102

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:48Z-1607105

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

text: Homo_sapiens-sc-2013-04-03T15:19:51Z-1607108

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Larval: protruding mouth: ZFS:0000035 - Larval: Day 4:ZFS:0000036

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_14_1_mutant-sc-2013-04-02T11:26:08Z-1605407

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_14_3_sibling-sc-2013-04-02T11:26:13Z-1605410

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_14_3_mutant-sc-2013-04-02T11:26:12Z-1605409

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 14 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: ZMP_phenotype_14_5_sibling-sc-2013-04-02T11:26:16Z-1605412

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 14 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph14

text: Danio rerio

text: mixed


text: Human

text: Promega

text: SC

text: public

characteristic: SRA accession

text: Homo_sapiens-sc-2013-04-03T15:14:30Z-1606919

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens

text: Promega


text: Human

text: Promega

text: SC

text: public

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