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text: male

text: Mus Musculus

text: SC

text: public

text: 2042STDY5252293-sc-2012-03-13T13:16:49Z-1309601

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2042STDY5261310-sc-2011-12-15T14:38:47Z-1320068

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: 2042STDY5252075-sc-2012-03-13T13:13:05Z-1309366

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2042STDY5261230-sc-2011-12-15T14:36:38Z-1319839

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

text: Mus Musculus

text: SC

text: public

text: 2042STDY5252081-sc-2012-03-13T13:13:18Z-1309380

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: 2042STDY5251977-sc-2012-03-13T13:12:51Z-1309352

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2042STDY5261085-sc-2011-12-15T14:33:33Z-1319407

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 2042STDY5261056-sc-2011-12-15T14:32:27Z-1319326

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

text: Mus Musculus

text: SC

text: public

text: 2042STDY5252141-sc-2012-03-13T13:14:57Z-1309481

text: house mouse

text: Mus musculus

text: Mus musculus


text: Hepatitis C virus

text: SC

text: public

text: 2821_HCV_4-sc-2011-12-14T16:51:33Z-1325215

text: Hepatitis C virus

text: cDNA

characteristic: sample description

text: Hepatitis C virus


text: Hepatitis C virus

text: SC

text: public

text: 2820_HCV_3-sc-2011-12-14T16:51:32Z-1325214

text: Hepatitis C virus

text: cDNA

text: Hepatitis C virus


text: Human immunodeficiency virus 1

text: SC

text: public

text: 2824_HIV1_3-sc-2011-12-14T16:51:35Z-1325218

text: Human immunodeficiency virus 1

text: cDNA

text: Human immunodeficiency virus 1


text: Human immunodeficiency virus 1

text: SC

text: public

text: 2825_HIV1_4-sc-2011-12-14T16:51:36Z-1325219

text: Human immunodeficiency virus 1

text: cDNA

text: Human immunodeficiency virus 1


text: Human immunodeficiency virus 1

text: SC

text: public

text: 2822_HIV1_1-sc-2011-12-14T16:51:33Z-1325216

text: Human immunodeficiency virus 1

text: cDNA

text: Human immunodeficiency virus 1


text: Human immunodeficiency virus 1

text: SC

text: public

text: 2823_HIV1_2-sc-2011-12-14T16:51:35Z-1325217

text: Human immunodeficiency virus 1

text: cDNA

text: Human immunodeficiency virus 1


text: Hepatitis C virus

text: SC

text: public

text: 2819_HCV_2-sc-2011-12-14T16:51:31Z-1325213

text: Hepatitis C virus

text: cDNA

text: Hepatitis C virus


text: Plasmodium berghei

text: NK65 ny

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Pb_NK65ny-sc-2012-01-18T15:50:40Z-1335804

text: Plasmodium berghei

text: Plasmodium berghei

text: NK65 ny

characteristic: strain


text: Plasmodium berghei

text: SC

text: public

text: Pb_NK65e-sc-2012-01-18T15:50:40Z-1335805

text: Plasmodium berghei

text: Plasmodium berghei


text: Plasmodium berghei

text: SP11 RLL

text: SC

text: public

text: Pb_SP11_RLL-sc-2012-01-18T15:50:42Z-1335807

text: Plasmodium berghei

text: Plasmodium berghei

text: SP11 RLL


text: Staphylococcus aureus

text: SC

text: public

text: LHI_Sa_52-sc-2012-11-08T15:54:05Z-1330158

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: Staphylococcus aureus

text: SC

text: public

text: LHI_Sa_42-sc-2012-11-08T15:53:52Z-1330148

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: Streptococcus agalactiae

text: SC

text: public

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: Streptococcus agalactiae

text: SC

text: public

text: GU874391-sc-2012-01-18T14:48:19Z-1335618

text: Streptococcus agalactiae

text: Streptococcus agalactiae


characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: anonymized name

characteristic: collection date

text: USA

characteristic: country

text: asymptomatic bacteriuria

characteristic: host status

text: urine

characteristic: isolation source

text: Streptococcus agalactiae

text: Streptococcus agalactiae

text: human

characteristic: specific host

characteristic: title


text: Streptococcus pyogenes

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: FIJI3142-sc-2012-01-18T14:49:10Z-1335688

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: SRA accession

text: FIJI3159-sc-2012-01-18T14:49:13Z-1335694

characteristic: Submitter Id

text: Streptococcus pyogenes

characteristic: organism

text: Streptococcus pyogenes

characteristic: strain


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3059-sc-2012-01-18T14:48:54Z-1335667

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3155-sc-2012-01-18T14:49:12Z-1335692

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3020-sc-2012-01-18T14:48:46Z-1335659

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3104-sc-2012-01-18T14:49:02Z-1335679

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3085-sc-2012-01-18T14:48:59Z-1335673

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3169-sc-2012-01-18T14:49:15Z-1335696

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3067-sc-2012-01-18T14:48:56Z-1335669

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3046-sc-2012-01-18T14:48:51Z-1335664

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3089-sc-2012-01-18T14:49:00Z-1335675

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3176-sc-2012-01-18T14:49:17Z-1335698

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3082-sc-2012-01-18T14:48:58Z-1335672

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3066-sc-2012-01-18T14:48:55Z-1335668

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus agalactiae

text: SC

text: public

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3052-sc-2012-01-18T14:48:53Z-1335666

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3107-sc-2012-01-18T14:49:03Z-1335680

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3148-sc-2012-01-18T14:49:10Z-1335689

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3158-sc-2012-01-18T14:49:13Z-1335693

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3072-sc-2012-01-18T14:48:57Z-1335670

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3025-sc-2012-01-18T14:48:48Z-1335661

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3051-sc-2012-01-18T14:48:52Z-1335665

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3043-sc-2012-01-18T14:48:50Z-1335663

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3099-sc-2012-01-18T14:49:00Z-1335676

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3138-sc-2012-01-18T14:49:08Z-1335686

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococcus pyogenes

text: SC

text: public

text: FIJI3152-sc-2012-01-18T14:49:12Z-1335691

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Shigella boydii

text: SC

text: public

text: Shigella boydii

text: Shigella boydii


text: Shigella sonnei

text: SC

text: public

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-CHECKLIST

text: SC

text: public

text: H1_1324_0269-sc-2012-01-23T15:06:57Z-1337641

characteristic: anonymized name

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Diseased

characteristic: host health state

text: Faeces

text: Shigella sonnei

text: Shigella sonnei

text: Human


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: H1_1222_0292-sc-2012-01-23T15:06:51Z-1337633

text: United Kingdom

text: Diseased

text: Faeces

characteristic: isolation source

text: Shigella sonnei

text: Shigella sonnei

text: Human

characteristic: specific host

characteristic: title


text: Shigella flexneri

characteristic: ArrayExpress-Species

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Shigella flexneri

text: Shigella flexneri


text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: South Africa

characteristic: country

text: South Africa

text: Diseased

text: Diseased

characteristic: host status

text: Human

text: Shigella flexneri

characteristic: pmid

text: Shigella flexneri

characteristic: serovar

text: Human

text: Shigella flexneri; Shigella flexneri


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: H1_1254_0448-sc-2012-01-23T15:06:48Z-1337629

characteristic: anonymized name

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Diseased

characteristic: host health state

text: Faeces

text: Shigella sonnei

characteristic: organism

text: Shigella sonnei

text: Human

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: H1_1262_0256-sc-2012-01-23T15:06:47Z-1337628

text: United Kingdom

text: Diseased

text: Faeces

characteristic: isolation source

text: Shigella sonnei

text: Shigella sonnei

text: Human

characteristic: specific host

characteristic: title


text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 561176_561185-sc-2012-01-23T15:06:24Z-1337597

characteristic: Submitter Id

text: South Africa

characteristic: country

text: South Africa

text: Diseased

text: Diseased

characteristic: host status

text: Human

text: Shigella flexneri

characteristic: pmid

text: Shigella flexneri

text: Human

text: Shigella flexneri; Shigella flexneri


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: H1_1262_0255-sc-2012-01-23T15:06:49Z-1337630

characteristic: anonymized name

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Diseased

characteristic: host health state

text: Faeces

text: Shigella sonnei

text: Shigella sonnei

text: Human


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: H1_1254_0446-sc-2012-01-23T15:06:50Z-1337632

text: United Kingdom

text: Diseased

text: Faeces

text: Shigella sonnei

characteristic: organism

text: Shigella sonnei

text: Human

characteristic: strain


text: Shigella sonnei

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 336217_28-sc-2012-01-23T15:05:51Z-1337557

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-CHECKLIST

text: SC

text: public

text: H1_1254_0447-sc-2012-01-23T15:06:50Z-1337631

characteristic: Submitter Id

text: United Kingdom

text: Diseased

text: Faeces

characteristic: isolation source

text: Shigella sonnei

text: Shigella sonnei

text: Human

characteristic: specific host

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: H1_1292_0487-sc-2012-01-23T15:06:54Z-1337637

characteristic: anonymized name

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Diseased

characteristic: host health state

text: Faeces

text: Shigella sonnei

text: Shigella sonnei

text: Human


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: H1_1308_0359-sc-2012-01-23T15:06:54Z-1337638

text: United Kingdom

text: Diseased

text: Faeces

text: Shigella sonnei

characteristic: organism

text: Shigella sonnei

text: Human


text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: H1_1302_0319-sc-2012-01-23T15:06:56Z-1337639

text: United Kingdom

text: Diseased

text: Faeces

text: Shigella sonnei

text: Shigella sonnei

text: Human

characteristic: strain


characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

text: H1_1270_0290-sc-2012-01-23T15:06:53Z-1337635

characteristic: Submitter Id

text: United Kingdom

text: Diseased

text: Faeces

text: Shigella sonnei

text: Shigella sonnei

text: Human


text: Shigella boydii

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Shigella boydii

text: Shigella boydii


text: Shigella sonnei

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Shigella sonnei

text: Shigella sonnei


text: Shigella flexneri

text: SC

text: public

text: E2610_72-sc-2012-01-23T15:06:37Z-1337615

text: Shigella flexneri

text: Shigella flexneri


text: SC

text: public

text: H1_1270_0291-sc-2012-01-23T15:06:53Z-1337636

characteristic: anonymized name

characteristic: collection date

text: United Kingdom

text: Diseased

text: Faeces

characteristic: isolation source

text: Shigella sonnei

text: Shigella sonnei

text: Human

characteristic: specific host

characteristic: title


text: Shigella flexneri

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: Shigella flexneri

text: Shigella flexneri


text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Diseased

characteristic: host health state

text: Diseased

characteristic: host status

text: Human

text: Shigella flexneri

characteristic: organism

characteristic: pmid

text: Shigella flexneri

characteristic: serovar

text: Human

text: Shigella flexneri; Shigella flexneri


characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: South Africa

characteristic: country

text: South Africa

characteristic: geographic location (country and/or sea)

text: Diseased

text: Diseased

text: Human

text: Shigella flexneri

text: Shigella flexneri

text: Human

characteristic: strain

text: Shigella flexneri; Shigella flexneri


text: Mouse

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC_MLMC5288404-sc-2012-02-09T08:46:20Z-1350709

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: Mouse

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SC_MLMC5288395-sc-2012-02-09T08:46:13Z-1350700

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288386-sc-2012-02-09T08:46:06Z-1350691

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288391-sc-2012-02-09T08:46:11Z-1350696

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288400-sc-2012-02-09T08:46:17Z-1350705

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288406-sc-2012-02-09T08:46:21Z-1350711

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288412-sc-2012-02-09T08:46:25Z-1350717

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288414-sc-2012-02-09T08:46:27Z-1350719

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288409-sc-2012-02-09T08:46:23Z-1350714

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288402-sc-2012-02-09T08:46:18Z-1350707

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288416-sc-2012-02-09T08:46:28Z-1350721

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288398-sc-2012-02-09T08:46:16Z-1350703

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288396-sc-2012-02-09T08:46:14Z-1350701

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288407-sc-2012-02-09T08:46:22Z-1350712

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288388-sc-2012-02-09T08:46:08Z-1350693

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288418-sc-2012-02-09T08:46:30Z-1350723

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288405-sc-2012-02-09T08:46:21Z-1350710

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288394-sc-2012-02-09T08:46:12Z-1350699

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288413-sc-2012-02-09T08:46:26Z-1350718

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288415-sc-2012-02-09T08:46:28Z-1350720

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288410-sc-2012-02-09T08:46:24Z-1350715

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288411-sc-2012-02-09T08:46:25Z-1350716

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288417-sc-2012-02-09T08:46:29Z-1350722

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288397-sc-2012-02-09T08:46:15Z-1350702

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288387-sc-2012-02-09T08:46:07Z-1350692

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288408-sc-2012-02-09T08:46:23Z-1350713

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mouse

text: SC

text: public

text: SC_MLMC5288399-sc-2012-02-09T08:46:16Z-1350704

text: house mouse

text: Mus musculus

text: Mus musculus


text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

text: BGI-SHENZHEN

text: public

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: hepatic carcinoma

characteristic: title


text: SRA-Webin

text: BGI-SHENZHEN

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: Homo sapiens

text: Homo sapiens

text: hepatic carcinoma


text: Mus Musculus

text: SC

text: public

text: 3GMUS5276403-sc-2012-01-25T14:20:38Z-1337450

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 3GMUS5291842-sc-2012-02-09T14:15:23Z-1354742

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 3GMUS5291843-sc-2012-02-09T14:15:24Z-1354743

text: house mouse

text: Mus musculus

text: Mus musculus


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

text: SC

text: public

text: 3GMUS5276405-sc-2012-01-25T14:20:40Z-1337452

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 3GMUS5291847-sc-2012-02-09T14:15:26Z-1354747

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

text: Mus Musculus

text: SC

text: public

text: 3GMUS5276404-sc-2012-01-25T14:20:39Z-1337451

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 3GMUS5291844-sc-2012-02-09T14:15:24Z-1354744

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: 3GMUS5276402-sc-2012-01-25T14:20:38Z-1337449

text: house mouse

text: Mus musculus

text: Mus musculus


name: E-MTAB-1031:Cell culture 1 GFP-KD RNA-PolII

text: public

tag: Namespace:Lausanne Genomic Technologies Facility (LGTF), University of Lausanne

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: fibroblast

characteristic: cell type

text: house mouse

text: Protocols: 3T3-L1 cell culture and differentiation: Mouse embryonic fibroblast-adipose like cells (cell line 3T3-L1) obtained from ATCC were maintained in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10% fetal calf serum (FCS; Amimed), 1X antibiotic solution (Invitrogen) and the cultures were incubated at 37 C and 5% CO2. The lentiviral mammalian vector pLKO.1 containing SMRT, NCoR1 and Kaiso-specific shRNAs (three shRNAs per target were used) along with control shRNA (empty pLKO.1 plasmid or GFP shRNA) were obtained from Sigma. Viral particles containing shRNA expression plasmid were generated in 293T cells using a CalPhos mammalian transfection kit (Clontech) according to . 293T cells were transfected with transfer plasmids containing SMRT or Kaiso-specific shRNAs or control shRNAs along with packaging plasmids (Pcmvr8.74 and PMd2.G) and the next day, the culture medium was refreshed and after 24h, viral particles along with medium were collected in 50 ml falcon tubes. Viral particle-containing medium was filtered with 0.45um centricon syringe filters and preserved at -80 C in small aliquots. 3T3-L1 cells at a density of 5 X 103 cells were transduced with viral particles containing a pool of three shRNA expression plasmids in 10cm petri-plates. After 72h of viral incubation, the medium was changed to a puromycin selection medium (2 ug/ml puromycin in complete DMEM medium) to select the stably transduced cells. After every two days, puromycin selection media was changed and the stably transduced cells were selected for one to two weeks before performing actual experiments. The control shRNA- transduced cells were treated similarly. Cells were collected from pre-adipocytes (D0) and at five distinct time points after induction of differentiation (2h, D1, D4, and D6). The cells were washed two times with 1X PBS and cross-linked using 1% formaldehyde for 10 min at room temperature followed by quenching the reaction using 125mM glycine for 5 min. After quenching, the petri-plates were placed on ice, cells were scraped using a cell scraper and collected in falcon tubes. The cells were then washed three times using cold 1X PBS and cell pellets were stored at -80 C until further use. The cells were lysed in nuclei extraction buffer (50mM HEPES-NaOH pH 7.5, 140mM NaCl, 1mM EDTA pH 8.0, 10% glycerol, 0.5% NP-40, 0.25% TritonX-100) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) for 10 min at 4 C while shaking to isolate the nuclei. The isolated nuclei were then washed using protein extraction buffer (200mM NaCl, 1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) at room temperature for 10 min. Washed nuclei were resuspended in chromatin extraction buffer (1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100) supplemented with protease and phosphatase inhibitor tablets (Roche) and incubated for 20 min on ice. The chromatin was fragmented using a Bioruptor (Diagenode) sonicator for 80 min using high amplitude and 30s ON & 30s OFF cycles to obtain 200-500 bp-sized fragments. A cooling unit was used to circulate the cold water during sonication to avoid de-crosslinking because of overheating. The fragmented chromatin was centrifuged at 17,000xg for 10 min and then clear supernatant was collected in chilled 15ml falcon tubes. The DNA concentration of the chromatin was estimated using a NanoDrop and the sonicated chromatin was diluted with ChIP dilution buffer (1mM EDTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100 containing protease and phosphatase inhibitors) to get 100 ug/ml of chromatin for each IP. BSA and ssDNA (Salmon Sperm DNA) -preblocked protein-A sepharose (80 ul/IP) beads were added to the samples and incubated for 2h to remove non-specific- binding chromatin. To the supernatant, 5 ul/IP rabbit polyclonal anti-SMRT antibody (Abcam, cat no.: ab-24551), anti-NCoR1 (Abcam, cat no.: ab-24552), or RNA Pol-II antibody (Santa Cruz, cat no.: sc9001) was added to immuno-precipitate the chromatin complex at 4 C overnight. After the overnight incubation, 50ul blocked beads were added to each sample and incubated for 90 min at 4 C to pull down the respective antibody-chromatin complexes. The beads were then washed four times with low salt wash buffer (20mM Tris-Cl pH 8.0, 150mM NaCl, 2mM EDTA pH 8.0, 0.1% SDS, 1% TritonX-100) followed by two washes with high salt wash buffer (20mM Tris-Cl pH 8.0, 500mM NaCl, 2mM EDTA pH 8.0, 0.1% SDS, 1% TritonX-100), lithium chloride wash buffer (10mM Tris-Cl pH 8.0, 0.25 M LiCl, 1mM EDTA pH 8.0, 1% NP-40, 1% sodium deoxycholate) and tris-EDTA (TE) buffer (10mM Tris-Cl pH 8.0, 1mM EDTA pH 8.0). After removing the wash buffer completely, SMRT-bound chromatin complexes were eluted from beads for 30 min using elution buffer (100mM sodium bicarbonate and 1% SDS in milliQ water). The eluted chromatin was then reverse-crosslinked by incubating the eluted supernatant at 65 C overnight on a heat block after adding 8ul of 5M NaCl. The next day, DNA was purified from the reverse-crosslinked chromatin by proteinase and RNase digestion followed by purification using Qiagen DNA purification columns. The purified DNA was eluted in 50ul of Qiagen elution buffer.

text: GFP-shRNA

characteristic: geneticmodification

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 1 GFP-KD RNA-PolII

type: has member


E-MTAB-1031:Cell culture 5 SMRT

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Lausanne Genomic Technologies Facility (LGTF), University of Lausanne

text: public

text: E-MTAB-1031:Cell culture 5 SMRT

text: ArrayExpress

text: fibroblast

text: house mouse

characteristic: common name

text: Protocols: 3T3-L1 cell culture and differentiation: Mouse embryonic fibroblast-adipose like cells (cell line 3T3-L1) obtained from ATCC were maintained in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10% fetal calf serum (FCS; Amimed), 1X antibiotic solution (Invitrogen) and the cultures were incubated at 37 C and 5% CO2. Cells were sub-cultured in 1:5 into new petri-plates when they were 75-80% confluent. 3T3-L1 pre-adipocytes at 2 days post-confluence were differentiated into adipocytes using differentiation inducing cocktail of 1uM Dexamethasone (Dex), 0.5mM isobutyl-methyl-xanthine (IBMX) and 167nM insulin in DMEM with 10% FCS. After two days of induction with differentiation medium, cells were washed with cell culture grade 1X phosphate buffered saline (PBS) and complete medium containing 167nM insulin was added. Two days thereafter, fresh DMEM medium containing FCS was added to the cells and at day six, cells were stained with oil red-O to estimate the extent of differentiation into mature fat cells. Cells were collected from pre-adipocytes (D0) and at five distinct time points after induction of differentiation (2h, D1, D4, and D6). The cells were washed two times with 1X PBS and cross-linked using 1% formaldehyde for 10 min at room temperature followed by quenching the reaction using 125mM glycine for 5 min. After quenching, the petri-plates were placed on ice, cells were scraped using a cell scraper and collected in falcon tubes. The cells were then washed three times using cold 1X PBS and cell pellets were stored at -80 C until further use. The cells were lysed in nuclei extraction buffer (50mM HEPES-NaOH pH 7.5, 140mM NaCl, 1mM EDTA pH 8.0, 10% glycerol, 0.5% NP-40, 0.25% TritonX-100) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) for 10 min at 4 C while shaking to isolate the nuclei. The isolated nuclei were then washed using protein extraction buffer (200mM NaCl, 1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) at room temperature for 10 min. Washed nuclei were resuspended in chromatin extraction buffer (1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100) supplemented with protease and phosphatase inhibitor tablets (Roche) and incubated for 20 min on ice. The chromatin was fragmented using a Bioruptor (Diagenode) sonicator for 80 min using high amplitude and 30s ON & 30s OFF cycles to obtain 200-500 bp-sized fragments. A cooling unit was used to circulate the cold water during sonication to avoid de-crosslinking because of overheating. The fragmented chromatin was centrifuged at 17,000xg for 10 min and then clear supernatant was collected in chilled 15ml falcon tubes. The DNA concentration of the chromatin was estimated using a NanoDrop and the sonicated chromatin was diluted with ChIP dilution buffer (1mM EDTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100 containing protease and phosphatase inhibitors) to get 100 ug/ml of chromatin for each IP. BSA and ssDNA (Salmon Sperm DNA) -preblocked protein-A sepharose (80 ul/IP) beads were added to the samples and incubated for 2h to remove non-specific- binding chromatin. To the supernatant, 5 ul/IP rabbit polyclonal anti-SMRT antibody (Abcam, cat no.: ab-24551), anti-NCoR1 (Abcam, cat no.: ab-24552), or RNA Pol-II antibody (Santa Cruz, cat no.: sc9001) was added to immuno-precipitate the chromatin complex at 4 C overnight. After the overnight incubation, 50ul blocked beads were added to each sample and incubated for 90 min at 4 C to pull down the respective antibody-chromatin complexes. The beads were then washed four times with low salt wash buffer (20mM Tris-Cl pH 8.0, 150mM NaCl, 2mM EDTA pH 8.0, 0.1% SDS, 1% TritonX-100) followed by two washes with high salt wash buffer (20mM Tris-Cl pH 8.0, 500mM NaCl, 2mM EDTA pH 8.0, 0.1% SDS, 1% TritonX-100), lithium chloride wash buffer (10mM Tris-Cl pH 8.0, 0.25 M LiCl, 1mM EDTA pH 8.0, 1% NP-40, 1% sodium deoxycholate) and tris-EDTA (TE) buffer (10mM Tris-Cl pH 8.0, 1mM EDTA pH 8.0). After removing the wash buffer completely, SMRT-bound chromatin complexes were eluted from beads for 30 min using elution buffer (100mM sodium bicarbonate and 1% SDS in milliQ water). The eluted chromatin was then reverse-crosslinked by incubating the eluted supernatant at 65 C overnight on a heat block after adding 8ul of 5M NaCl. The next day, DNA was purified from the reverse-crosslinked chromatin by proteinase and RNase digestion followed by purification using Qiagen DNA purification columns. The purified DNA was eluted in 50ul of Qiagen elution buffer.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 5 SMRT


E-MTAB-1031:Cell culture 1 SMRT

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-1031:Cell culture 1 SMRT

text: ArrayExpress

text: fibroblast

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 1 SMRT


name: E-MTAB-1031:Cell culture 2 SMRT-KD RNA-PolII

characteristic: INSDC last update

text: public

text: ArrayExpress

text: fibroblast

text: house mouse

text: SMRT-shRNA

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: Cell culture 2 SMRT-KD RNA-PolII


E-MTAB-1031:Cell culture 2 SMRT

text: public

text: E-MTAB-1031:Cell culture 2 SMRT

text: ArrayExpress

text: fibroblast

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 2 SMRT

characteristic: title


E-MTAB-1031:Cell culture 4 SMRT

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1031:Cell culture 4 SMRT

text: ArrayExpress

text: fibroblast

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 4 SMRT


E-MTAB-1031:Cell culture 2 NCoR1

text: public

text: E-MTAB-1031:Cell culture 2 NCoR1

characteristic: Submitter Id

text: ArrayExpress

text: fibroblast

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 2 NCoR1


E-MTAB-1031:Cell culture 1 NCoR1

text: public

text: E-MTAB-1031:Cell culture 1 NCoR1

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 1 NCoR1


E-MTAB-1031:Cell culture 3 SMRT

text: public

text: E-MTAB-1031:Cell culture 3 SMRT

text: ArrayExpress

characteristic: cell line

text: fibroblast

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 3 SMRT


E-MTAB-1031:Cell culture 1 input

text: public

text: E-MTAB-1031:Cell culture 1 input

text: ArrayExpress

text: fibroblast

characteristic: cell type

text: house mouse

text: Protocols: 3T3-L1 cell culture and differentiation: Mouse embryonic fibroblast-adipose like cells (cell line 3T3-L1) obtained from ATCC were maintained in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10% fetal calf serum (FCS; Amimed), 1X antibiotic solution (Invitrogen) and the cultures were incubated at 37 C and 5% CO2. Cells were sub-cultured in 1:5 into new petri-plates when they were 75-80% confluent. 3T3-L1 pre-adipocytes at 2 days post-confluence were differentiated into adipocytes using differentiation inducing cocktail of 1uM Dexamethasone (Dex), 0.5mM isobutyl-methyl-xanthine (IBMX) and 167nM insulin in DMEM with 10% FCS. After two days of induction with differentiation medium, cells were washed with cell culture grade 1X phosphate buffered saline (PBS) and complete medium containing 167nM insulin was added. Two days thereafter, fresh DMEM medium containing FCS was added to the cells and at day six, cells were stained with oil red-O to estimate the extent of differentiation into mature fat cells. Cells were collected from pre-adipocytes (D0) and at five distinct time points after induction of differentiation (2h, D1, D4, and D6). The cells were washed two times with 1X PBS and cross-linked using 1% formaldehyde for 10 min at room temperature followed by quenching the reaction using 125mM glycine for 5 min. After quenching, the petri-plates were placed on ice, cells were scraped using a cell scraper and collected in falcon tubes. The cells were then washed three times using cold 1X PBS and cell pellets were stored at -80 C until further use. The cells were lysed in nuclei extraction buffer (50mM HEPES-NaOH pH 7.5, 140mM NaCl, 1mM EDTA pH 8.0, 10% glycerol, 0.5% NP-40, 0.25% TritonX-100) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) for 10 min at 4 C while shaking to isolate the nuclei. The isolated nuclei were then washed using protein extraction buffer (200mM NaCl, 1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0) supplemented with a protease inhibitor tablet (Roche) and phosphatase inhibitors (5mM NaF, 1mM ?-glycerol phosphate and 1mM sodium orthovanadate) at room temperature for 10 min. Washed nuclei were resuspended in chromatin extraction buffer (1mM EDTA pH 8.0, 0.5mM EGTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100) supplemented with protease and phosphatase inhibitor tablets (Roche) and incubated for 20 min on ice. The chromatin was fragmented using a Bioruptor (Diagenode) sonicator for 80 min using high amplitude and 30s ON & 30s OFF cycles to obtain 200-500 bp-sized fragments. A cooling unit was used to circulate the cold water during sonication to avoid de-crosslinking because of overheating. The fragmented chromatin was centrifuged at 17,000xg for 10 min and then clear supernatant was collected in chilled 15ml falcon tubes. The DNA concentration of the chromatin was estimated using a NanoDrop and the sonicated chromatin was diluted with ChIP dilution buffer (1mM EDTA pH 8.0, 10mM Tris-HCl pH 8.0 and 1% TritonX-100 containing protease and phosphatase inhibitors) to get 100 ug/ml of chromatin for each IP. 1% of chromatin was saved as a control Input sample. The chromatin was then reverse-crosslinked by incubating the eluted supernatant at 65 C overnight on a heat block after adding 8ul of 5M NaCl. The next day, DNA was purified from the reverse-crosslinked chromatin by proteinase and RNase digestion followed by purification using Qiagen DNA purification columns. The purified DNA was eluted in 50ul of Qiagen elution buffer.

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Cell culture 1 input


text: SC

text: public

text: Ryukyu mouse

text: Mus caroli

text: Mus caroli