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text: ArrayExpress

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text: red flour beetle

characteristic: common name

text: Protocols: T. castaneum was reared in a dry and dark condition (a relative humidity 60 plus/minus 5%) at room temperature (25 plus/minus 10C) with wheat flour (Pareve, USA). Fully grown late instar larvae (5 mm) were used in this study. A full open reading frame of CpBV-H4 and the truncated CpBV-H4 (without N-terminal 38 amino acids) were cloned into pIB eukaryotic expression vector (Invitrogen, Carlsbad, CA, USA) according to previous studies . The recombinant pIB vector was mixed with Metafectene PRO transfection reagent (Biontex, Planegg, Germany) according to manufacturers instruction. Briefly, 0.5 ug of recombinants were mixed with 3 uL of Metafectene reagent and incubated for 20 min at room temperature to allow DNA-lipid complexes to be formed before injection into hemocoel of late instar larvae of T. castaneum. Glass capillary (World Precision Instruments, Sarasota, FL, USA) injection needles were made using a micropipette puller PN30 (Narishige, Tokyo, Japan). A total 60 nL was injected into the larval hemocoel at the rate 10 nL/sec using a micro-syringe pump controller (WPI) under a microscope (Olympus S730, Tokyo, Japan). After 48 h PI, total RNA was extracted using Trizol reagent (Invitrogen) and followed by reverse transcription using RT-Premix (Bioneer, Daejeon, Korea) with an oligo dT and subsequent RNase H (1 unit/uL) treatment. The synthesized cDNA was used as a template for PCR amplification. Control PCR reaction was performed with RNA extract template, which was used as a template to check absence of DNA contamination. Briefly, 100 larvae each of Tribolium castaneum injected with recombinant CpBV-H4 and truncated CpBV-H4 were homogenized in PBS on ice, followed by the addition of formaldehyde to a final concentration of 1% and incubation at 37C for 10 min. Chromatin immunoprecipitation assays were performed using a QuikChIP kit (IMGENEX) according to the instruction manual The precipitated DNA was dissolved in Tris-EDTA buffer and read by an Illumina using paired end sequencing.

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text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:23Z-1488965

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:24Z-1488966

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:25Z-1488967

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:26Z-1488968

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:27Z-1488969

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:28Z-1488970

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:29Z-1488971

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:30Z-1488972

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:31Z-1488973

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:32Z-1488974

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:33Z-1488975

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:34Z-1488976

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:35Z-1488977

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:36Z-1488978

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:37Z-1488979

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:38Z-1488980

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:39Z-1488981

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:40Z-1488982

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:41Z-1488983

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:42Z-1488984

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:43Z-1488985

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:44Z-1488986

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:45Z-1488987

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:46Z-1488988

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:47Z-1488989

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:48Z-1488990

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:49Z-1488991

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:50Z-1488992

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:51Z-1488993

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:52Z-1488994

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:53Z-1488995

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:54Z-1488996

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:55Z-1488997

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:56Z-1488998

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:57Z-1488999

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:58Z-1489000

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:12:59Z-1489001

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:00Z-1489002

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:01Z-1489003

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:02Z-1489004

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:03Z-1489005

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:04Z-1489006

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:05Z-1489007

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:06Z-1489008

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:07Z-1489009

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:08Z-1489010

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:09Z-1489011

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:10Z-1489012

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:11Z-1489013

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:12Z-1489014

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:13Z-1489015

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:14Z-1489016

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:15Z-1489017

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:16Z-1489018

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:17Z-1489019

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:18Z-1489020

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:19Z-1489021

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:20Z-1489022

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:21Z-1489023

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:22Z-1489024

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:23Z-1489025

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:24Z-1489026

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:25Z-1489027

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:26Z-1489028

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:27Z-1489029

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:28Z-1489030

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:29Z-1489031

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:30Z-1489032

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:31Z-1489033

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:32Z-1489034

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:33Z-1489035

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:34Z-1489036

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:37Z-1489037

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:38Z-1489038

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:39Z-1489039

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:40Z-1489040

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:41Z-1489041

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:42Z-1489042

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:43Z-1489043

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:44Z-1489044

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Human

text: SC

text: public

text: Homo_sapiens-sc-2013-08-28T17:13:45Z-1489045

text: human

text: Homo sapiens

text: genomic DNA

text: Homo sapiens


text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Spleenic Folicular B cells

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: 16h Perit Monocytes

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S13_region_1-sc-2013-08-29T13:46:50Z-1686231

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S14_region_1-sc-2013-08-29T13:46:52Z-1686232

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S15_region_1-sc-2013-08-29T13:46:53Z-1686233

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S13_region_2-sc-2013-08-29T13:47:11Z-1686246

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S14_region_2-sc-2013-08-29T13:47:12Z-1686247

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S15_region_2-sc-2013-08-29T13:47:13Z-1686248

text: house mouse

text: Mus musculus

text: Spleenic Folicular B-2 cell

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text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP1-sc-2013-08-29T14:06:30Z-1686249

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2013UknMP9-sc-2013-08-29T14:06:33Z-1686250

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP17-sc-2013-08-29T14:06:34Z-1686251

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB111-sc-2013-08-29T14:06:35Z-1686252

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB60-sc-2013-08-29T14:06:37Z-1686253

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_6_2-sc-2013-08-29T14:06:38Z-1686254

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB20_PB21-sc-2013-08-29T14:06:40Z-1686255

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB40_PB41-sc-2013-08-29T14:06:41Z-1686256

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB120_PB61-sc-2013-08-29T14:06:42Z-1686257

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB77_PB78-sc-2013-08-29T14:06:43Z-1686258

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB124_PB94-sc-2013-08-29T14:06:45Z-1686259

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB109_PB110-sc-2013-08-29T14:06:46Z-1686260

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP2-sc-2013-08-29T14:06:47Z-1686261

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2013UknMP10-sc-2013-08-29T14:06:48Z-1686262

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP18-sc-2013-08-29T14:06:49Z-1686263

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB112-sc-2013-08-29T14:06:50Z-1686264

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB117-sc-2013-08-29T14:06:51Z-1686265

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_6_3-sc-2013-08-29T14:06:52Z-1686266

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB22_PB23-sc-2013-08-29T14:06:53Z-1686267

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB125_PB44-sc-2013-08-29T14:06:54Z-1686268

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB62_PB63-sc-2013-08-29T14:06:56Z-1686269

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB79_PB80-sc-2013-08-29T14:06:57Z-1686270

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB95_PB96-sc-2013-08-29T14:06:58Z-1686271

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB121_PB122-sc-2013-08-29T14:06:59Z-1686272

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP3-sc-2013-08-29T14:07:00Z-1686273

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2014UknMP11-sc-2013-08-29T14:07:02Z-1686274

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP19-sc-2013-08-29T14:07:03Z-1686275

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB113-sc-2013-08-29T14:07:04Z-1686276

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB11-sc-2013-08-29T14:07:05Z-1686277

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_12-sc-2013-08-29T14:07:06Z-1686278

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB24_PB25-sc-2013-08-29T14:07:07Z-1686279

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB45_PB15-sc-2013-08-29T14:07:08Z-1686280

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB64_PB65-sc-2013-08-29T14:07:09Z-1686281

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB81_PB82-sc-2013-08-29T14:07:10Z-1686282

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB97_PB98-sc-2013-08-29T14:07:11Z-1686283

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB123_PB38-sc-2013-08-29T14:07:12Z-1686284

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP4-sc-2013-08-29T14:07:13Z-1686285

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP12-sc-2013-08-29T14:07:14Z-1686286

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_15_3-sc-2013-08-29T14:07:15Z-1686287

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB42-sc-2013-08-29T14:07:17Z-1686289

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB26_PB126-sc-2013-08-29T14:07:20Z-1686291

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB118_PB50-sc-2013-08-29T14:07:20Z-1686292

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB66_PB67-sc-2013-08-29T14:07:21Z-1686293

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB83_PB84-sc-2013-08-29T14:07:23Z-1686294

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB99_PB100-sc-2013-08-29T14:07:24Z-1686295

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB27_PB3-sc-2013-08-29T14:07:25Z-1686296

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP5-sc-2013-08-29T14:07:26Z-1686297

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP13-sc-2013-08-29T14:07:27Z-1686298

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB33-sc-2013-08-29T14:07:29Z-1686299

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_3_1-sc-2013-08-29T14:07:31Z-1686301

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB9_PB19-sc-2013-08-29T14:07:32Z-1686302

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB28_PB30-sc-2013-08-29T14:07:33Z-1686303

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB51_PB52-sc-2013-08-29T14:07:34Z-1686304

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB68_PB70-sc-2013-08-29T14:07:35Z-1686305

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB85_PB8-sc-2013-08-29T14:07:36Z-1686306

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB101_PB102-sc-2013-08-29T14:07:37Z-1686307

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: NegCtrl_1-sc-2013-08-29T14:07:39Z-1686308

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP6-sc-2013-08-29T14:07:40Z-1686309

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP14-sc-2013-08-29T14:07:41Z-1686310

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB59-sc-2013-08-29T14:07:42Z-1686311

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_3_2-sc-2013-08-29T14:07:44Z-1686313

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB12_PB13-sc-2013-08-29T14:07:45Z-1686314

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB31_PB34-sc-2013-08-29T14:07:46Z-1686315

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB53_PB54-sc-2013-08-29T14:07:47Z-1686316

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB71_PB72-sc-2013-08-29T14:07:48Z-1686317

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB87_PB115-sc-2013-08-29T14:07:49Z-1686318

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB103_PB104-sc-2013-08-29T14:07:50Z-1686319

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: NegCtrl_2-sc-2013-08-29T14:07:51Z-1686320

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP7-sc-2013-08-29T14:07:52Z-1686321

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP15-sc-2013-08-29T14:07:53Z-1686322

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB69-sc-2013-08-29T14:07:54Z-1686323

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB106-sc-2013-08-29T14:07:55Z-1686324

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_3_3-sc-2013-08-29T14:07:56Z-1686325

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB16_PB17-sc-2013-08-29T14:07:57Z-1686326

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB35_PB36-sc-2013-08-29T14:07:58Z-1686327

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB55_PB56-sc-2013-08-29T14:08:00Z-1686328

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB73_PB74-sc-2013-08-29T14:08:01Z-1686329

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB89_PB90-sc-2013-08-29T14:08:02Z-1686330

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB105_PB16-sc-2013-08-29T14:08:03Z-1686331

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_15_1-sc-2013-08-29T14:08:04Z-1686332

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2013UknMP8-sc-2013-08-29T14:08:05Z-1686333

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 2012UKnMP16-sc-2013-08-29T14:08:06Z-1686334

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB116-sc-2013-08-29T14:08:07Z-1686335

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB3_PB10-sc-2013-08-29T14:08:08Z-1686336

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_6_1-sc-2013-08-29T14:08:09Z-1686337

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB18_PB114-sc-2013-08-29T14:08:10Z-1686338

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB37_PB14-sc-2013-08-29T14:08:11Z-1686339

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB57_PB58-sc-2013-08-29T14:08:12Z-1686340

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB75_PB76-sc-2013-08-29T14:08:13Z-1686341

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB91_PB92-sc-2013-08-29T14:08:14Z-1686342

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: PB107_PB108-sc-2013-08-29T14:08:15Z-1686343

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: Plasmodium falciparum

text: NF54-PiggyBac Mutant

text: SC

text: public

text: 40PBPld_15_2-sc-2013-08-29T14:08:16Z-1686344

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: NF54-PiggyBac Mutant


text: public

tag: Namespace:Dept of Clinical and Experimental Medicine, Linksping University, Sweden

text: ArrayExpress

characteristic: broker name

text: platelet

characteristic: cell type

text: human

text: Protocols: The local Regional Ethical Review Board (Link_ping, Sweden) granted an ethical permission for this study. Unstimulated non-irradiated apheresis platelets were collected from two healthy human male and one healthy female blood donor. Platelets were collected by COBE Spectra system (Gambro BCT) and were used on the same collection day. Residual leukocytes were depleted with anti-CD45 conjugated Dynabeads, according to the manufacturers recommendations (Pan Leukocyte; Invitrogen, Carlsbad, CA). The platelet suspension, with a volume of 30 mL and a platelet count of 1.4 * 10exp9 cells/mL, was centrifuged at 800 g for 8 min and the supernatant was discarded. The platelet pellet was processed for leukocyte depletion, as recommended by the supplier of Pan Leukocyte reagent (Invitrogen, Carlsbad, CA). The leukocyte removal was performed at room temperature. Approximately 70 _ 75% of the original platelets were recovered after the leukocyte depletion. To investigate the level of leukocyte contamination, we determined the level of CD45 (PTPRC) transcript by qPCR using TaqMan Gene Expression Assay for this gene according to the recommendations of the supplier (Assay ID: Hs00894713_m1; Applied Bioystems, Carlsbad, CA, USA). For isolation of total RNA, we employed the miRVana RNA Extraction Kit as recommended by the supplier (Life Technologies). Ribosomal RNA was depleted with Ribo-Zero (Epicentre, Madison, WI, USA) and strand specific barcoded RNA-sequencing libraries were prepared using ScriptSeq v2 (Epicentre) according to manufacturers instructions.

characteristic: description

text: RNA Extract S2

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Dept of Clinical and Experimental Medicine, Linksping University, Sweden

text: public

text: ArrayExpress

text: platelet

text: human

characteristic: common name

text: RNA Extract S1

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: male

text: fresh specimen


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: platelet

text: human

text: RNA Extract S3

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

text: fresh specimen


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: aTox_resistant_mESC_gLib1-sc-2013-08-30T13:51:20Z-1686807

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: PCR products

characteristic: sample description

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: aTox_resistant_mESC_gLib2-sc-2013-08-30T13:51:28Z-1686808

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: Danio rerio

text: TL/AB

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: SL6_11_A1-sc-2013-08-30T14:16:04Z-1621946

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB

characteristic: strain


text: Danio rerio

text: TL/AB

characteristic: ENA-CHECKLIST

text: SC

text: public

text: SL6_11_B1-sc-2013-08-30T14:16:07Z-1621947

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SL6_11_C1-sc-2013-08-30T14:16:08Z-1621948

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL6_11_D1-sc-2013-08-30T14:16:10Z-1621949

text: zebrafish

characteristic: common name

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB