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text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: SL7_T_H12-sc-2013-08-30T14:18:00Z-1622041

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Ni2Nod2_A1-sc-2013-08-30T14:18:01Z-1622042

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Ni1Nod2_B1-sc-2013-08-30T14:18:02Z-1622043

text: goldfish

text: Carassius auratus

text: Exome

text: Carassius auratus

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Ni05Nod2_C1-sc-2013-08-30T14:18:03Z-1622044

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Na2Nod2_D1-sc-2013-08-30T14:18:04Z-1622045

text: grass carp

text: Ctenopharyngodon idella

text: Exome

text: Ctenopharyngodon idella

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Na1Nod2_E1-sc-2013-08-30T14:18:06Z-1622046

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Na05Nod2_F1-sc-2013-08-30T14:18:07Z-1622047

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Ni1Alb_G1-sc-2013-08-30T14:18:08Z-1622048

text: common carp

text: Cyprinus carpio

text: Exome

text: Cyprinus carpio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Ni05Alb_H1-sc-2013-08-30T14:18:10Z-1622049

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Na1Alb_A2-sc-2013-08-30T14:18:11Z-1622050

text: zebrafish

text: Danio rerio

text: Exome

text: Danio rerio

text: TL/AB


text: Danio rerio

text: TL/AB

text: SC

text: public

text: Na05Alb_B2-sc-2013-08-30T14:18:12Z-1622051

text: bighead carp

text: Hypophthalmichthys nobilis

text: Exome

text: Hypophthalmichthys nobilis

text: TL/AB


text: Cancer Research UK Cambridge Institute

text: public

tag: Namespace:Cancer Research UK Cambridge Institute

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial

characteristic: cell type

text: human

text: Protocols: The MCF-7, MDA-MB-231, MDA-MB-453, LNCaP and ZR-75-1 cell lines were obtained from American Type Culture Collection (ATCC). Cells were grown in DMEM (MCF-7, MDA-MB-231 and MDA-MB-453) or RPMI (LNCaP and ZR-75-1), both supplemented with 10% Fetal Bovine Serum, 2 mM L-glutamine, 50U/ml penicillin and 50ug/ml streptomycin in 37_C incubator with 5% CO2. For all experiments, 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt et al., Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001). The ChIP assays were performed as previously described (Schmidt et al., 2009). Protein-bound DNA was immunoprecipitated with antibodies against COT2/COUP-TFII (R&D Systems, PP-H7147-00), FoxA1 (Abcam, ab5089), ER (Santa-Cruz, sc-543), FoxA2 (Santa-Cruz, sc-6554) and P300 (Santa-Cruz, sc-585). For the ChIP-exo library preparation, the immunoprecipitated chromatin was end-repaired, ligated to the TruSeq P7 exo-adapter, nick repaired, digested with the lambda and the RecJf exonucleases. The digested DNA was extracted, second-strand synthesised with the P7 primer, ligated to the TruSeq P5 exo-adapter and finally amplified with 18 PCR cycles. A 2% agarose gel was used to select 200-300 bp DNA fragments.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: title

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: ArrayExpress

text: hepatocyte

text: house mouse

text: Protocols: The liver material was isolated from three adult (three month) C57/BL6 males obtained from Charles River UK. The investigation was approved by the ethics committee and followed the Cambridge Institute guidelines for the use of animals in experimental studies under home office license PPL80/2197. All tissues were treated post mortem with 1% formaldehyde as previously described (Schmidt et al., Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001). The ChIP assays were performed as previously described (Schmidt et al., 2009). Protein-bound DNA was immunoprecipitated with antibodies against COT2/COUP-TFII (R&D Systems, PP-H7147-00), FoxA1 (Abcam, ab5089), ER (Santa-Cruz, sc-543), FoxA2 (Santa-Cruz, sc-6554) and P300 (Santa-Cruz, sc-585). For the ChIP-exo library preparation, the immunoprecipitated chromatin was end-repaired, ligated to the TruSeq P7 exo-adapter, nick repaired, digested with the lambda and the RecJf exonucleases. The digested DNA was extracted, second-strand synthesised with the P7 primer, ligated to the TruSeq P5 exo-adapter and finally amplified with 18 PCR cycles. A 2% agarose gel was used to select 200-300 bp DNA fragments.

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

text: fresh specimen


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: LNCaP

text: epithelial

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

text: fresh specimen

text: LNCaP


text: public

tag: Namespace:University of Rome La Sapienza. Dipartimento di Biotecnologie Cellulari ed matologia, Sezione di Genetica Molecolare

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: HeLaS3 cells were grown in DMEM supplemented with 10%FBS, pen/strep and L-glutamine. HeLaS3 cells were transfected with 10nMol/l siRNA using Interferin reagent (Polyplus) according to manufacturer instructions. AGO2 reduction was confirmed by Western blot analysis. (1) Pellet 2x 106 cells . (2) Add 300uL of Permeabilization buffer (15mM Tris-HCl pH 7.4, 300mM sucrose, 60 mM KCl, 15 mM naCl, 4mM CaCl2, 0.5mM EGTA, 0.2%NP-40, 0.5mM beta-mercaptoethanol) on ice. (3) Add 50U of Mnase. (4) Incubate for 20 min at 37 degrees C (HeLa S3). (5) Stop reaction by adding 300uL of Stop Buffer (50mM Tris-HCl pH 8, 20mM EDTA, 1%SDS), place the tube on ice and wait 2 min. (6) RNase treatment: add 75uG RNase A 1h at 37 degrees C. (7) Proteinase K treatment: add 30uG RNase A 1h at 55 degrees C. (8) DNA was purified by adding 1 volume of phenol:chloroform:isoamyl alchol, vortex and centrifuge max speed 5-10 min 4 degrees C . Repeat. Add 1 volume of chloroform, vortex and centrifuge max speed 5-10 min 4 degrees C .

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: frozen specimen


characteristic: ENA-CHECKLIST

text: University of Rome La Sapienza. Dipartimento di Biotecnologie Cellulari ed matologia, Sezione di Genetica Molecolare

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: frozen specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Manchester

text: public

tag: Namespace:University of Manchester

text: ArrayExpress

text: HeLa

text: epitheloid cervix carcinoma cell

characteristic: cell type

text: human

text: Protocols: The preparation of the material was as described in Elsby et al., 2009. HeLa cells were crosslinked and frozen in order to pool enough for ChIP. Elsby,L.M., Donn,R., Alourfi,Z., Green,L.M., Beaulieu,E. and Ray, D.W. (2009) Hypoxia and glucocorticoid signaling converge to regulate macrophage migration inhibitory factor gene expression. Arthritis Rheum., 60, 2220-2231. ChIP assays were performed as described in Elsby et al., 2009, with the following modifications for ChIP-seq. The crosslinked material was sheared by twice probe sonication (SONICS VCX130) with energy more than 50% as 12 secs on/ 40secs off, followed by water sonication using bio-rupter (Next Generation) for 40 mins, at high level, 30 secs on/ 30 secs off, 4 degrees C. The size of sheared DNA fragments was between 100 bp to 500 bp, with most was around 200 bp. Immunoprecipitation was performed by incubating 1x10^7cells with 3 ug of GR antibodies (1ug anti-GR M-20 and 1 ug anti-GR H-300X from Santa Cruz Biotechnology, and 1 ug anti-GR from Sigma-Aldrich), overnight at 4 degrees C, followed by protein G dynabeads (Invitrogen). Elsby,L.M., Donn,R., Alourfi,Z., Green,L.M., Beaulieu,E. and Ray, D.W. (2009) Hypoxia and glucocorticoid signaling converge to regulate macrophage migration inhibitory factor gene expression. Arthritis Rheum., 60, 2220-2231.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex


text: TGAC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: bread wheat

text: Wheat CS42 CSS 2AL flow-sorted DNA

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: Wheat CS42 CSS 2AL flow-sorted DNA


text: TGAC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: bread wheat

text: Wheat CS42 CSS 2AS flow-sorted DNA

text: Triticum aestivum

text: Triticum aestivum

text: Wheat CS42 CSS 2AS flow-sorted DNA


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_25_1_sibling-sc-2013-09-04T09:01:31Z-1687311

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 25 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_25_2_mutant-sc-2013-09-04T09:01:33Z-1687312

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 25 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_25_2_sibling-sc-2013-09-04T09:01:34Z-1687313

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 25 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_25_3_mutant-sc-2013-09-04T09:01:35Z-1687314

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 25 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_25_3_sibling-sc-2013-09-04T09:01:37Z-1687315

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 25 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_25_4_mutant-sc-2013-09-04T09:01:39Z-1687316

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 25 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_25_4_sibling-sc-2013-09-04T09:01:40Z-1687317

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 25 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_25_5_mutant-sc-2013-09-04T09:01:41Z-1687318

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 25 clutch 5. A 5 base indexing sequence (AACCG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_25_5_sibling-sc-2013-09-04T09:01:43Z-1687319

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 25 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph25

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_27_1_mutant-sc-2013-09-04T09:06:20Z-1687320

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 27 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_27_1_sibling-sc-2013-09-04T09:06:23Z-1687321

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 27 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_27_2_mutant-sc-2013-09-04T09:06:25Z-1687322

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 27 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_27_2_sibling-sc-2013-09-04T09:06:26Z-1687323

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 27 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_27_3_mutant-sc-2013-09-04T09:06:27Z-1687324

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 27 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_27_3_sibling-sc-2013-09-04T09:06:28Z-1687325

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 27 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_27_4_mutant-sc-2013-09-04T09:06:30Z-1687326

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 27 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_27_4_sibling-sc-2013-09-04T09:06:31Z-1687327

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 27 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_27_5_mutant-sc-2013-09-04T09:06:33Z-1687328

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 27 clutch 5. A 5 base indexing sequence (AACCG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_27_5_sibling-sc-2013-09-04T09:06:34Z-1687329

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 27 clutch 5. A 5 base indexing sequence (ACGGG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph27

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_29_1_mutant-sc-2013-09-04T09:06:35Z-1687330

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 29 clutch 1. A 5 base indexing sequence (GAGGC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_29_1_sibling-sc-2013-09-04T09:06:36Z-1687331

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 29 clutch 1. A 5 base indexing sequence (AGAAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

text: ZMP_phenotype_29_2_mutant-sc-2013-09-04T09:06:37Z-1687332

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 29 clutch 2. A 5 base indexing sequence (CAGAG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZMP_phenotype_29_2_sibling-sc-2013-09-04T09:06:39Z-1687333

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 29 clutch 2. A 5 base indexing sequence (GCACG) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole Embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_29_3_mutant-sc-2013-09-04T09:06:40Z-1687334

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 29 clutch 3. A 5 base indexing sequence (CGCAA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_29_3_sibling-sc-2013-09-04T09:06:41Z-1687335

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 29 clutch 3. A 5 base indexing sequence (CAAGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_29_4_mutant-sc-2013-09-04T09:06:43Z-1687336

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from morphologically abnormal embryos from ZMP phenotype 29 clutch 4. A 5 base indexing sequence (GCCGA) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

text: Danio rerio

text: mixed


text: Hatching : Long pec - ZFS:0000033

text: Whole Embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_29_4_sibling-sc-2013-09-04T09:06:44Z-1687337

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from morphologically normal embryos from ZMP phenotype 29 clutch 4. A 5 base indexing sequence (CGGCC) is bases 6 to 10 of read 1 followed by polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph29

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: female

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: Oocyte

text: Mus musculus


text: male

text: Mus Musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: HL1_9H_18-sc-2013-09-04T09:33:09Z-1687339

text: house mouse

text: Mus musculus

text: mESC

text: Mus musculus


text: male

text: Mus Musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: HL1_9H_19-sc-2013-09-04T09:33:34Z-1687340

text: house mouse

text: Mus musculus

characteristic: organism

text: mESC

text: Mus musculus


text: Mus Musculus

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HL1_9E_21-sc-2013-09-04T09:36:16Z-1687341

text: house mouse

text: Mus musculus

text: Control

text: Mus musculus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', pre-infiltration, biological replicate 1

characteristic: description

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', pre-infiltration, biological replicate 2

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', pre-infiltration, biological replicate 3

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 1h after infiltration with flg22, biological replicate 1

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 1h after infiltration with flg22, biological replicate 2

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 1h after infiltration with flg22, biological replicate 3

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 3h after infiltration with flg22, biological replicate 1

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 3h after infiltration with flg22, biological replicate 2

text: Brassica napus

text: Brassica napus


text: TGAC

text: public

text: rape

text: Brassica napus cv. 'Temple', 3h after infiltration with flg22, biological replicate 3

text: Brassica napus

text: Brassica napus


text: CRUK

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: MCF-7 cells [American Type Culture Collection (ATCC)] were grown in DMEM with 2mM L-glutamine, 10% fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. LY2 cells (kind gift from R. Clarke, Georgetown, Washington D.C.) were grown in phenol red-free MEM with 2mM L-glutamine, 10% charcoal dextrin stripped fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. Cells were steroid depleted for 3 days prior to treatment with Tamoxifen for 45 minutes. Proteins were cross linked to DNA by treating with formaldehyde (1%) for 10 minutes (ER) or 60 minutes (HMGB2). Chromatin immunoprecipitation and preparation of libraries was carried out as described in Schmidt et al (2009) Methods 48:240-248.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: frozen specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-CHECKLIST

text: CRUK

text: public

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

text: Protocols: MCF-7 cells [American Type Culture Collection (ATCC)] were grown in DMEM with 2mM L-glutamine, 10% fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. LY2 cells (kind gift from R. Clarke, Georgetown, Washington D.C.) were grown in phenol red-free MEM with 2mM L-glutamine, 10% charcoal dextrin stripped fetal bovine serum, 50U/ml penicillin and 50ug/ml streptomycin in 37C incubator with 5% CO2. Input libraries were harvested without further treatment. Proteins were cross linked to DNA by treating with formaldehyde (1%) for 10 minutes (ER) or 60 minutes (HMGB2). Chromatin immunoprecipitation and preparation of libraries was carried out as described in Schmidt et al (2009) Methods 48:240-248.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: frozen specimen


text: Homo sapiens

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Ce-M-M-, Research Center for Molecular Medicine of the Austrian Academy of Sciences

text: ArrayExpress

text: human

text: Protocols: genomic DNA was extracted from blood samples using standard procedures Libraries were generated using the NEBNext DNA Sample Prep kit (New England Biolabs) or the TruSeq SamplePrep V2 kit (Illumina) following the manufacturers instructions. Please note that the library for sample_30 was generated as paired-end and then sequenced twice, first with 51bp paired-end reads, and then a second time on a different flowcell with 51 bp single-end reads.

text: post-polycythemia vera acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens


text: Ce-M-M-, Research Center for Molecular Medicine of the Austrian Academy of Sciences

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: post-primary myelofibrosis acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: human

text: polycythemia vera

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: title


text: public

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: human

text: post-myelodysplastic syndrome acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens


text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: 72h_res_B_1_Mac_A-sc-2013-09-09T14:04:55Z-1648319

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: 72h_inflam_B_1_Mac_A-sc-2013-09-09T14:04:58Z-1648320

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 72h_B_2_A-sc-2013-09-09T14:04:59Z-1648321

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_B_1_A-sc-2013-09-09T14:05:00Z-1648322

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_res_B_1_Mac_B-sc-2013-09-09T14:05:01Z-1648323

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_inflam_B_1_Mac_B-sc-2013-09-09T14:05:02Z-1648324

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_B_2_B-sc-2013-09-09T14:05:03Z-1648325

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_B_1_B-sc-2013-09-09T14:05:04Z-1648326

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_res_B_1_Mac_C-sc-2013-09-09T14:05:05Z-1648327

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_inflam_B_1_Mac_C-sc-2013-09-09T14:05:06Z-1648328

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_B_2_C-sc-2013-09-09T14:05:07Z-1648329

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: 72h_B_1_C-sc-2013-09-09T14:05:08Z-1648330

text: house mouse

text: Mus musculus

text: Mus musculus


text: Institut Pasteur

text: public

tag: Namespace:Institut Pasteur

text: ArrayExpress

text: house mouse

text: Protocols: All experiments involving mice were handled in accordance with the Pasteur Institute guidelines for animal welfare. Only 9- to 12-week-old female C57BL6/J conventional (Charles River) and germfree wild type mice mice (CDTA) were used for experiments. Germ-free mice were housed in plastic gnotobiotic isolators. L. monocytogenes overnight cultures were diluted in BHI and bacteria were grown until OD600nm=1. Bacterial cultures were centrifuged at 3,500 x g for 15 min. After three washes in PBS, L. monocytogenes pellet was resuspended in PBS at a final concentration of 2.5 x 1010 bacteria/mL. Mice were infected orally with 5 x 109 bacteria diluted in 200 uL of PBS supplemented with 300 uL of CaCO3 (50 mg/mL) for 24 h and 72h. Serial dilutions of the inoculum were plated to control the number of L. monocytogenes inoculated in mice. RNAs from the ileal tissue were extracted and purified using classical TRIzol/chloroform protocol. All samples were treated with Turbo DNase (Ambion). RNA quality was determined using Experion Automated Electrophoresis Station (Bio-Rad). The purified small RNA was ligated with 3' RNA adaptor (5'-/5rApp/ATCTCGTATGCCGTCTTCTGCTTG/3ddC/), which is specifically modified to target miRNAs and other small RNAs that have a 3'hydroxyl group. The 5' RNA adaptor (5'-GUUCAGAGUUCUACAGUCCGACGAUC) was then ligated to the 5' phosphate end of small RNA. Reverse transcription was done to convert the RNA to cDNA, which was then selectively enriched by 12 cycles of PCR. The PCR products were purified on 5% PAGE (BioRad) in a size range between 90-105 base pairs and checked on a Bioanalyser DNA1000 chip (Agilent).

text: germ-free

characteristic: growth condition

text: uninfected

characteristic: infect

text: Mus musculus

text: Mus musculus

text: ileum

characteristic: organism part

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

text: 0 hour

characteristic: time


characteristic: ENA-CHECKLIST

text: Institut Pasteur

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: conventional

text: Listeria monocytogenes infected

text: Mus musculus

text: Mus musculus

text: ileum

text: Mus musculus

text: fresh specimen

text: 24 hour


characteristic: ENA-FIRST-PUBLIC

text: Institut Pasteur

text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: germ-free

text: uninfected

text: Mus musculus

text: Mus musculus

text: ileum

text: Mus musculus

text: fresh specimen

text: 24 hour

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut Pasteur

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: conventional

text: uninfected

text: Mus musculus

text: Mus musculus

text: ileum

text: Mus musculus

text: fresh specimen

text: 0 hour


text: Institut Pasteur

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: conventional

characteristic: growth condition

text: Listeria monocytogenes infected

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: ileum

text: Mus musculus

text: fresh specimen

text: 72 hour


characteristic: ENA-CHECKLIST

text: Institut Pasteur

text: public

text: ArrayExpress

text: house mouse

text: germ-free

text: uninfected

text: Mus musculus

text: Mus musculus

text: ileum

characteristic: organism part

text: Mus musculus

text: fresh specimen

characteristic: specimen with known storage state

text: 72 hour

characteristic: time


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Lentiviral_mouse_whole_genome_gRNA_library_verion_1_preparation_2-sc-2013-09-12T11:21:12Z-1691994

text: house mouse

text: Mus musculus

text: PCR products

characteristic: sample description

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Mouse_whole_genome_gRNA_v1_mESC_library_1-sc-2013-09-12T11:21:15Z-1691995

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Mouse_whole_genome_gRNA_v1_mESC_library_2-sc-2013-09-12T11:21:21Z-1691996

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: Wildtype

characteristic: ArrayExpress-Genotype

text: bone marrow

characteristic: ArrayExpress-OrganismPart

text: Mus Musculus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: NPM1_WT1-sc-2013-09-12T12:36:06Z-1692093

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA from Lin- bone marrow cells

text: Mus musculus


text: Mutant (het)

text: bone marrow

text: Mus Musculus

text: SC

text: public

text: NPM1_Mut1-sc-2013-09-12T12:36:09Z-1692094

text: house mouse

text: Mus musculus

text: RNA from Lin- bone marrow cells

text: Mus musculus


text: INRA

text: public

text: tomato

text: Micro-Tom

characteristic: cultivar

text: Solanum lycopersicum

text: Solanum lycopersicum

text: Micro-Tom MM

characteristic: strain

text: leaves

characteristic: tissue lib

text: Micro-Tom MM genome DNA

characteristic: title


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: gRNA_site_1_against_26_GPI_genes_transfected_rep1-sc-2013-09-12T15:06:16Z-1692095

text: house mouse

text: Mus musculus

text: PCR products

characteristic: sample description

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_transfected_rep1-sc-2013-09-12T15:06:15Z-1692096

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: gRNA_site_1_against_26_GPI_genes_control_rep1-sc-2013-09-12T15:06:16Z-1692097

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_control_rep1-sc-2013-09-12T15:06:17Z-1692098

text: house mouse

text: Mus musculus

characteristic: organism

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_1_against_26_GPI_genes_transfected_rep2-sc-2013-09-12T15:06:18Z-1692099

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_transfected_rep2-sc-2013-09-12T15:06:19Z-1692100

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_1_against_26_GPI_genes_control_rep2-sc-2013-09-12T15:06:20Z-1692101

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_control_rep2-sc-2013-09-12T15:06:22Z-1692102

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: Danio rerio

text: SC

text: public

text: CH73_51A13-sc-2013-09-13T11:21:14Z-1692199

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D624C6-sc-2013-09-13T11:21:17Z-1692200

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D745I3-sc-2013-09-13T11:21:18Z-1692201

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_36P13-sc-2013-09-13T11:21:19Z-1692202

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_124H16-sc-2013-09-13T11:21:20Z-1692203

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_156A17-sc-2013-09-13T11:21:21Z-1692204

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_195F6-sc-2013-09-13T11:21:22Z-1692205

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_20O19-sc-2013-09-13T11:21:23Z-1692206

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_155G19-sc-2013-09-13T11:21:24Z-1692207

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_83N12-sc-2013-09-13T11:21:25Z-1692208

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_13H20-sc-2013-09-13T11:21:26Z-1692209

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_194A16-sc-2013-09-13T11:21:28Z-1692211

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_310J17-sc-2013-09-13T11:21:29Z-1692212

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_70E20-sc-2013-09-13T11:21:30Z-1692213

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_91H18-sc-2013-09-13T11:21:31Z-1692214

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_764B11-sc-2013-09-13T11:21:32Z-1692215

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D216L18-sc-2013-09-13T11:21:33Z-1692216

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D268F16-sc-2013-09-13T11:21:34Z-1692217

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1098D12-sc-2013-09-13T11:21:35Z-1692218

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D226N6-sc-2013-09-13T11:21:36Z-1692219

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D327B24-sc-2013-09-13T11:21:37Z-1692220

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D407F15-sc-2013-09-13T11:21:38Z-1692221

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D412B24-sc-2013-09-13T11:21:39Z-1692222

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D434G14-sc-2013-09-13T11:21:40Z-1692223

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D453B11-sc-2013-09-13T11:21:41Z-1692224

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D528F18-sc-2013-09-13T11:21:42Z-1692225

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D709H18-sc-2013-09-13T11:21:43Z-1692226

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D717I20-sc-2013-09-13T11:21:44Z-1692227

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D770P23-sc-2013-09-13T11:21:45Z-1692228

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1370A6-sc-2013-09-13T11:21:46Z-1692229

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1720F8-sc-2013-09-13T11:21:47Z-1692230

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_2436E5-sc-2013-09-13T11:21:48Z-1692231

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_247D12-sc-2013-09-13T11:21:49Z-1692232

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D824B23-sc-2013-09-13T11:21:49Z-1692233

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D888I10-sc-2013-09-13T11:21:50Z-1692234

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D902I8-sc-2013-09-13T11:21:51Z-1692235

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D924K14-sc-2013-09-13T11:21:53Z-1692237

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D925N5-sc-2013-09-13T11:21:54Z-1692238

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D926I8-sc-2013-09-13T11:21:55Z-1692239

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_229A22-sc-2013-09-13T11:21:56Z-1692240

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_77E17-sc-2013-09-13T11:21:58Z-1692243

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_85D8-sc-2013-09-13T11:21:59Z-1692244

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_260A21-sc-2013-09-13T11:22:00Z-1692245

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1055F12-sc-2013-09-13T11:22:01Z-1692246

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D236G7-sc-2013-09-13T11:22:02Z-1692247

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_98A18-sc-2013-09-13T11:22:03Z-1692248

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_160L20-sc-2013-09-13T11:22:05Z-1692249

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_190B4-sc-2013-09-13T11:22:06Z-1692250

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_230I6-sc-2013-09-13T11:22:07Z-1692251

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_238F17-sc-2013-09-13T11:22:08Z-1692252

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_25I6-sc-2013-09-13T11:22:09Z-1692253

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_283M13-sc-2013-09-13T11:22:10Z-1692254

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_285K16-sc-2013-09-13T11:22:11Z-1692255

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_288A9-sc-2013-09-13T11:22:11Z-1692256

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_43N23-sc-2013-09-13T11:22:12Z-1692257

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_57E22-sc-2013-09-13T11:22:13Z-1692258

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_87E4-sc-2013-09-13T11:22:14Z-1692259

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_93L3-sc-2013-09-13T11:22:15Z-1692260

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_98G2-sc-2013-09-13T11:22:16Z-1692261

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_p106G3-sc-2013-09-13T11:22:17Z-1692262

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: DKEY_p115D6-sc-2013-09-13T11:22:17Z-1692263

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_85N17-sc-2013-09-13T11:22:18Z-1692264

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH73_348P3-sc-2013-09-13T11:22:19Z-1692265

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_161P16-sc-2013-09-13T11:22:20Z-1692266

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_223N20-sc-2013-09-13T11:22:21Z-1692267

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_241N12-sc-2013-09-13T11:22:22Z-1692268

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: CH211_79D17-sc-2013-09-13T11:22:23Z-1692269

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1546H10-sc-2013-09-13T11:22:24Z-1692270

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_1674G5-sc-2013-09-13T11:22:24Z-1692271

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_2039A5-sc-2013-09-13T11:22:25Z-1692272

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_2783G6-sc-2013-09-13T11:22:26Z-1692273

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D267D19-sc-2013-09-13T11:22:27Z-1692274

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D274B18-sc-2013-09-13T11:22:28Z-1692275

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D276M13-sc-2013-09-13T11:22:28Z-1692276

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D515O14-sc-2013-09-13T11:22:30Z-1692277

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D684D12-sc-2013-09-13T11:22:30Z-1692278

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D768L20-sc-2013-09-13T11:22:32Z-1692279

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D769B16-sc-2013-09-13T11:22:32Z-1692280

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D780J19-sc-2013-09-13T11:22:33Z-1692281

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D780K4-sc-2013-09-13T11:22:34Z-1692282

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D790C21-sc-2013-09-13T11:22:35Z-1692283

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D798L13-sc-2013-09-13T11:22:36Z-1692284

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D814B15-sc-2013-09-13T11:22:37Z-1692285

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D81M20-sc-2013-09-13T11:22:38Z-1692286

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D824M7-sc-2013-09-13T11:22:39Z-1692287

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D838E12-sc-2013-09-13T11:22:40Z-1692288

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D844F13-sc-2013-09-13T11:22:41Z-1692289

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D849B8-sc-2013-09-13T11:22:42Z-1692290

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D860M5-sc-2013-09-13T11:22:43Z-1692291

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D866D21-sc-2013-09-13T11:22:44Z-1692292

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Danio rerio

text: SC

text: public

text: zFOS_D870P3-sc-2013-09-13T11:22:45Z-1692293

text: zebrafish

text: Danio rerio

text: Danio rerio


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Mus musculus

text: SC

text: public

text: JM8_TV_1-sc-2013-09-16T11:16:21Z-1692598

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_2-sc-2013-09-16T11:16:23Z-1692599

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_3-sc-2013-09-16T11:16:24Z-1692600

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_4-sc-2013-09-16T11:16:25Z-1692601

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_5-sc-2013-09-16T11:16:26Z-1692602

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_6-sc-2013-09-16T11:16:27Z-1692603

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_7-sc-2013-09-16T11:16:28Z-1692604

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_8-sc-2013-09-16T11:16:30Z-1692605

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_9-sc-2013-09-16T11:16:31Z-1692606

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_10-sc-2013-09-16T11:16:32Z-1692607

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_11-sc-2013-09-16T11:16:33Z-1692608

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_12-sc-2013-09-16T11:16:34Z-1692609

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_13-sc-2013-09-16T11:16:35Z-1692610

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_14-sc-2013-09-16T11:16:36Z-1692611

text: house mouse

text: Mus musculus

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: JM8_TV_15-sc-2013-09-16T11:16:37Z-1692612

text: house mouse

text: Mus musculus

text: Mus musculus


text: CNRS

text: public

text: ArrayExpress

characteristic: broker name

text: European seabass

text: Protocols: Seabasses were sacrificed at midnight and midday. Brain and livers were collected and were stored in RNAlater (Invitrogen, Saint-Aubin, France) at 4C overnight. RNAlater was then removed and samples were stored at -80C until use. Total RNA from liver and brain was isolated using TRIzol (Invitrogen, Carlsbad, CA). Samples were treated with the DNA-free kit (Invitrogen, Saint-Aubin, France) using the manufacturers protocol. Total RNA was measured with a Nanodrop2000 (Thermo Scientific, Villebon-sur-Yvette, France). Total RNA pools of brain and liver were used for RNA-seq. Double stranded cDNA preparation and sequencing were performed at the BioPuces Strasbourg Department of the Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC Strasbourg, France). Sample preparation was performed using the Illumina mRNA sample preparation kit (Cat.No. RS-930-1001), involving the following steps. Poly-A containing mRNA were isolated from 4 ug of total RNA. The mRNA was then fragmented using divalent cations under elevated temperature (incubation time was reduced in order to obtain longer fragments). The cleaved RNA fragments were reverse transcribed into first stranded cDNA using random primers, second strand cDNA was then synthesized. Adaptors were ligated to the end-repaired cDNA. At the purification step, we selected two size ranges of templates: a pool of 350 and a pool of 500 base pair (bp) long. From then on, these two banks were treated separately for downstream enrichment by PCR and for paired-end RNA-seq. One flow cell was dedicated to each organ, and to each fragment size, i.e., a total of four flow cells (half a run) were employed.

characteristic: description

text: wild type genotype

characteristic: genotype

text: Dicentrarchus labrax

text: Dicentrarchus labrax

text: brain

characteristic: organism part

text: Dicentrarchus labrax

text: fresh specimen

characteristic: specimen with known storage state


text: CNRS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: European seabass

text: wild type genotype

text: Dicentrarchus labrax

text: Dicentrarchus labrax

text: liver

text: Dicentrarchus labrax

text: fresh specimen

characteristic: title


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: 6TG_resistant_mESC_gLib1-sc-2013-09-16T11:41:18Z-1692613

text: house mouse

text: Mus musculus

text: PCR products

characteristic: sample description

text: Mus musculus


text: male

text: Mus musculus

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 6TG_resistant_mESC_gLib2-sc-2013-09-16T11:41:20Z-1692614

characteristic: Submitter Id

text: house mouse

characteristic: common name

text: Mus musculus

text: PCR products

text: Mus musculus


text: Xenopus tropicalis

characteristic: ENA-CHECKLIST

text: SC

characteristic: INSDC last update

text: public

text: LZisoT332_1-sc-2013-09-17T14:16:08Z-1677436

text: western clawed frog

text: Xenopus (Silurana) tropicalis

characteristic: organism

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: LZisoT332_2-sc-2013-09-17T14:16:11Z-1677437

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: LZisoT340-sc-2013-09-17T14:16:12Z-1677438

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: LZisoT341-sc-2013-09-17T14:16:13Z-1677439

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: LZisoT408_1-sc-2013-09-17T14:16:14Z-1677440

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Xenopus tropicalis

text: SC

text: public

text: LZisoT408_2-sc-2013-09-17T14:16:15Z-1677441

text: western clawed frog

text: Xenopus (Silurana) tropicalis

text: Toe clip DNA prep

text: Xenopus (Silurana) tropicalis


text: Mus Musculus

text: SC

text: public

text: TMBD10_BS-sc-2013-09-18T12:28:21Z-1692906

text: house mouse

text: Mus musculus

text: DNA from ESC

text: Mus musculus


text: Mus Musculus

text: SC

text: public

text: TMBD10_oxBS-sc-2013-09-18T12:28:31Z-1692907

text: house mouse

text: Mus musculus

text: DNA from ESC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC27_BS-sc-2013-09-18T12:28:40Z-1692908

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC27_oxBS-sc-2013-09-18T12:29:46Z-1692911

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC28_oxBS-sc-2013-09-18T12:29:53Z-1692912

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC29_oxBS-sc-2013-09-18T12:30:03Z-1692913

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC19_BS-sc-2013-09-18T12:30:10Z-1692914

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC28_BS-sc-2013-09-18T12:30:17Z-1692909

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC29_BS-sc-2013-09-18T12:30:24Z-1692910

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC20_oxBS-sc-2013-09-18T12:30:31Z-1692919

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC37_oxBS-sc-2013-09-18T12:30:37Z-1692920

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC38_oxBS-sc-2013-09-18T12:30:43Z-1692921

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC20_BS-sc-2013-09-18T12:31:06Z-1692915

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC37_BS-sc-2013-09-18T12:31:14Z-1692916

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC38_BS-sc-2013-09-18T12:31:20Z-1692917

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: iPSC19_oxBS-sc-2013-09-18T12:31:26Z-1692918

text: house mouse

text: Mus musculus

text: DNA from iPSC

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: pMEFWt_BS-sc-2013-09-18T12:31:34Z-1692922

text: house mouse

text: Mus musculus

text: DNA from MEF

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: pMEFAIDKO_BS-sc-2013-09-18T12:31:41Z-1692923

text: house mouse

text: Mus musculus

text: DNA from MEF

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: pMEFWt_oxBS-sc-2013-09-18T12:31:48Z-1692924

text: house mouse

text: Mus musculus

text: DNA from MEF

text: Mus musculus


text: female

text: Mus Musculus

text: SC

text: public

text: pMEFAIDKO_oxBS-sc-2013-09-18T12:31:54Z-1692925

text: house mouse

text: Mus musculus

text: DNA from MEF

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib1_1st_round_4MOI-sc-2013-09-18T13:07:04Z-1692977

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib2_1st_round_4MOI-sc-2013-09-18T13:07:12Z-1692978

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib1_2nd_round_4MOI-sc-2013-09-18T13:07:29Z-1692979

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib1_2nd_round_10MOI-sc-2013-09-18T13:07:41Z-1692980

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib2_2nd_round_4MOI-sc-2013-09-18T13:07:46Z-1692981

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: Flu_resistant_mESC_gLib2_2nd_round_10MOI-sc-2013-09-18T13:07:54Z-1692982

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: BALB_10kb_nextera_1-sc-2013-09-18T13:54:26Z-1692983

text: house mouse

text: Mus musculus

text: DNA

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: C3H_10kb_nextera_1-sc-2013-09-18T13:54:33Z-1692984

text: house mouse

text: Mus musculus

text: DNA

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: NOD_10kb_nextera_1-sc-2013-09-18T13:54:39Z-1692985

text: house mouse

text: Mus musculus

text: DNA

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: NZO_10kb_nextera_1-sc-2013-09-18T13:54:46Z-1692986

text: house mouse

text: Mus musculus

text: DNA

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S3_region_1-sc-2013-08-29T13:46:39Z-1686221

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S6_region_1-sc-2013-08-29T13:46:42Z-1686224

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S9_region_1-sc-2013-08-29T13:46:46Z-1686227

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S12_region_1-sc-2013-08-29T13:46:49Z-1686230

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S3_region_2-sc-2013-08-29T13:46:58Z-1686236

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S6_region_2-sc-2013-08-29T13:47:01Z-1686239

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S9_region_2-sc-2013-08-29T13:47:04Z-1686242

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S12_region_2-sc-2013-08-29T13:47:07Z-1686245

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S2_region_1-sc-2013-08-29T13:46:38Z-1686220

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S5_region_1-sc-2013-08-29T13:46:41Z-1686223

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S8_region_1-sc-2013-08-29T13:46:45Z-1686226

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S11_region_1-sc-2013-08-29T13:46:48Z-1686229

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S2_region_2-sc-2013-08-29T13:46:55Z-1686235

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S5_region_2-sc-2013-08-29T13:47:00Z-1686238

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S8_region_2-sc-2013-08-29T13:47:03Z-1686241

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S11_region_2-sc-2013-08-29T13:47:06Z-1686244

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S1_region_1-sc-2013-08-29T13:46:37Z-1686219

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S4_region_1-sc-2013-08-29T13:46:40Z-1686222

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S7_region_1-sc-2013-08-29T13:46:44Z-1686225

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S10_region_1-sc-2013-08-29T13:46:47Z-1686228

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S1_region_2-sc-2013-08-29T13:46:54Z-1686234

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP- Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S4_region_2-sc-2013-08-29T13:46:58Z-1686237

text: house mouse

text: Mus musculus

text: Naïve Peritoneal YFP+ Macrophage

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S7_region_2-sc-2013-08-29T13:47:02Z-1686240

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-1 cell

text: Mus musculus


text: Mus musculus

text: SC

text: public

text: S10_region_2-sc-2013-08-29T13:47:05Z-1686243

text: house mouse

text: Mus musculus

text: Naïve Peritoneal B-2 cell

text: Mus musculus


text: public

tag: Namespace:Physiological Genomics and Medicine Group, Imperial College London

text: 6 week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Norway rat

text: Protocols: Rats were housed in an air-conditioned animal facility and allowed free access to standard laboratory chow and water. All experiments were done in agreement with the Animal Protection Law of the Czech Republic and were approved by the Ethics Committee of the Institute of Physiology, Czech Academy of Sciences, Prague. We collected left ventricular tissue from unfasted males from six week old male BN-Lx/Cub (referred to in this study as BN), SHR/Olalpcv (referred to as SHR) (n = 4 per strain); and from (BNxSHR)F1 and (SHRxBN)F1 (n = 4 per cross); and from male rats from 29 BXH/HXB recombinant inbred strains (n = 2 per strain) (Pravenec et al. 1989) between 9:00am and 10:00am. Left ventricles were snap frozen in liquid nitrogen and stored at -80°C. The BN-Lx/Cub and SHR/Olalpcv lines have been inbred over more than 80 generations (Simonis et al. 2012). RNA was extracted from 25mg of crushed left ventricular tissue from four BN and four SHR animals without pooling, using Trizol (Invitrogen) according to manufacturer's instructions. 4ug of total RNA was used to generate RNA-seq libraries using TruSeq RNA kit (Illumina) according to manufacturer's instructions.

characteristic: description

text: Rattus norvegicus

text: Rattus norvegicus

text: left ventricle

characteristic: organism part

text: Rattus norvegicus

text: frozen specimen

characteristic: specimen with known storage state

text: SHR/Olalpcv

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: Physiological Genomics and Medicine Group, Imperial College London

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 6 week

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left ventricle

text: Rattus norvegicus

text: frozen specimen

text: BN-Lx/Cub

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 6 week

text: ArrayExpress

text: Norway rat

text: Rattus norvegicus

text: Rattus norvegicus

text: left ventricle

text: Rattus norvegicus

text: frozen specimen

text: SHR/Olalpcv


text: IPK-Gatersleben

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: GABIPD

text: rye

characteristic: common name

text: DNA of chromosome 2R from Wheat-rye disomic addition lines ‘Chinese Spring’/‘Imperial’ obtained by flow-sorting from synchronized root tips according to Kubalakova et al. (2003, Genome 46: 893-905). DNA was amplified by multiple displacement amplification (MDA).

text: synchronized root tips from 1-2 weeks old seedlings

characteristic: isolation source

text: Secale cereale

text: Secale cereale

text: genomic DNA of Rye Chromosome 2R


text: IPK-Gatersleben

characteristic: INSDC last update

text: public

text: GABIPD

text: rye

text: DNA of chromosome 3R from Wheat-rye disomic addition lines ‘Chinese Spring’/‘Imperial’ obtained by flow-sorting from synchronized root tips according to Kubalakova et al. (2003, Genome 46: 893-905). DNA was amplified by multiple displacement amplification (MDA).

text: Secale cereale

characteristic: organism

text: Secale cereale

text: genomic DNA of Rye Chromosome 3R


text: IPK-Gatersleben

text: public

text: GABIPD

characteristic: broker name

text: rye

text: DNA of chromosome 4R from Wheat-rye disomic addition lines ‘Chinese Spring’/‘Imperial’ obtained by flow-sorting from synchronized root tips according to Kubalakova et al. (2003, Genome 46: 893-905). DNA was amplified by multiple displacement amplification (MDA).

characteristic: description

text: Secale cereale

text: Secale cereale

text: genomic DNA of Rye Chromosome 4R


text: IPK-Gatersleben

text: public

text: GABIPD

text: rye

text: DNA of chromosome 5R from Wheat-rye disomic addition lines ‘Chinese Spring’/‘Imperial’ obtained by flow-sorting from synchronized root tips according to Kubalakova et al. (2003, Genome 46: 893-905). DNA was amplified by multiple displacement amplification (MDA).

text: Secale cereale

text: Secale cereale

text: genomic DNA of Rye Chromosome 5R