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text: Protocols: Standard lab conditions were used to grow the HeLa cells used for these experiments HeLa cells were grown over night in 6 cm plates and transiently transfected with 300 pmol siRNA using Lipofectamine RNAiMAX (Life Technologies). 24 h post transfection the cells were split 1:2 in 10 cm plates and the day after transfected again with 600 pmol siRNA. RNA samples were obtained from XRN1 knockdown HeLa cells transfected with the indicated reporter mRNA expressing plasmids (TPI-WT or -PTCs). Total RNA was extracted using Isol-RNA-Lysis Reagent. 3' fragments were linker ligated, reverse transcribed and specific PCR products were amplified with gene specific primers.

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text: Protocols: Virus Strains: A/Mallard/British Columbia/500/2005 (H5N2) was used for LPAI challenge. A/Vietnam/1203/2004 (H5N1) was used for HPAI challenge. The viruses were grown in the allantoic cavities of 10-day-old embryonated chicken eggs for 48 hours at 35 degrees C. Allantoic fluid containing virus was harvested and stored at -80 degrees C until use. Virus yield was determined as 50% egg infectious dose (EID50) per millilitre of virus stock by the method of Reed and Muench. Experimental Animals: Specific pathogen-free white leghorn chickens were purchased from Charles River Laboratories (North Franklin, CT). All experiments involving animals were approved by the Animal Care and Use Committee of St. Jude Children's Research Hospital and performed in compliance with relevant policies of the National Institutes of Health and the Animal Welfare Act. All animal challenge experiments were performed in an animal biosafety level 2 containment facilities for the LPAI challenges and in biosafety level 3 enhanced containment laboratories for the HPAI challenges. Viral Challenge: Chickens, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of LPAI A/Mallard/British Columbia/500/2005 (H5N2) in phosphate buffered saline (PBS). Chickens, except the mock infection control group, were challenged with 101.5 EID50 intranasally, intratracheally, and intraocularly of HPAI A/Vietnam/1203/2004 (H5N1) in PBS. During LPAI and HPAI infections in chickens and ducks, mock infection control groups were also inoculated. Mock infection control birds received an equivalent volume and route of administration with PBS. Animals were monitored daily for clinical signs. Tissue Sampling: Lung and ileum samples were collected from all birds on days 1 and 3 dpi. Tissue homogenates were inoculated into 10-day-old embryonated chicken eggs in triplicate to screen for positive samples. Positive samples were then titrated in eggs and expressed as log10EID50/mL. The lower limit of detection was 0.75 log10EID50/mL. For each ileum and lung tissue, the tissue sample was homogenized in Trizol (Invitrogen Life Technologies), 0.1g tissue/1.0ml Trizol with handheld homogenizer Power Gen125 (Thermo Fisher Scientific). After homogenates were prepared, the samples were centrifuged to separate the liquid phases of Trizol homogenates. 0.5ml of the aqueous phase was passed through a QIagen RNeasy (Qiagen, Valencia, CA) spin column according to manufacturer's instructions for RNA extraction. Samples were frozen and stored at -80 degrees C until all samples were collected for analysis. Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.

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text: Protocols: Virus Strains. A/Mallard/British Columbia/500/2005 (H5N2) was used for LPAI challenge. A/Vietnam/1203/2004 (H5N1) was used for HPAI challenge. The viruses were grown in the allantoic cavities of 10-day-old embryonated chicken eggs for 48 hours at 35 degrees C. Allantoic fluid containing virus was harvested and stored at -80 degrees C until use. Virus yield was determined as 50% egg infectious dose (EID50) per millilitre of virus stock by the method of Reed and Muench .Experimental Animals. Domestic Gray Mallards were purchased from Ideal Poultry (Cameron, TX). All experiments involving animals were approved by the Animal Care and Use Committee of St. Jude Childrenâ€™s Research Hospital and performed in compliance with relevant policies of the National Institutes of Health and the Animal Welfare Act. All animal challenge experiments were performed in an animal biosafety level 2 containment facilities for the LPAI challenges and in biosafety level 3 enhanced containment laboratories for the HPAI challenges. Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of LPAI A/Mallard/British Columbia/500/2005 (H5N2) in phosphate buffered saline (PBS). Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of HPAI A/Vietnam/1203/2004 (H5N1) in PBS. During LPAI and HPAI infections in ducks, mock infection control groups were also inoculated. Mock infection control birds received an equivalent volume and route of administration with PBS. Animals were monitored daily for clinical signs. : For each ileum and lung tissue, the tissue sample was homogenized in Trizol (Invitrogen Life Technologies), 0.1g tissue/1.0ml Trizol with handheld homogenizer Power Gen125 (Thermo Fisher Scientific). After homogenates were prepared, the samples were centrifuged to separate the liquid phases of Trizol homogenates. 0.5ml of the aqueous phase was passed through a QIagen RNeasy (Qiagen, Valencia, CA) spin column according to manufacturer's instructions for RNA extraction. Samples were frozen and stored at -80 degrees C until all samples were collected for analysis. Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.

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text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_ileum_1_rep2.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_lung_1_rep2.fastq.gz

text: ArrayExpress

text: mallard

characteristic: infect

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

characteristic: organism part

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_lung_3_rep3.fastq.gz

text: ArrayExpress

characteristic: broker name

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day

characteristic: time


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_lung_3_rep1.fastq.gz

text: ArrayExpress

text: mallard

characteristic: common name

characteristic: description

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


characteristic: ENA-CHECKLIST

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_ileum_1_rep2.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

characteristic: organism

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day


characteristic: ENA-FIRST-PUBLIC

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_ileum_1_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_ileum_1_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_3_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2909:Smith_Roslin_APL_LP_lung_1_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

characteristic: SRA accession

text: E-MTAB-2909:Smith_Roslin_APL_LP_lung_3_rep3.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_ileum_3_rep2.fastq.gz

text: ArrayExpress

text: mallard

text: Protocols: Virus Strains. A/Mallard/British Columbia/500/2005 (H5N2) was used for LPAI challenge. A/Vietnam/1203/2004 (H5N1) was used for HPAI challenge. The viruses were grown in the allantoic cavities of 10-day-old embryonated chicken eggs for 48 hours at 35 degrees C. Allantoic fluid containing virus was harvested and stored at -80 degrees C until use. Virus yield was determined as 50% egg infectious dose (EID50) per millilitre of virus stock by the method of Reed and Muench .Experimental Animals. Domestic Gray Mallards were purchased from Ideal Poultry (Cameron, TX). All experiments involving animals were approved by the Animal Care and Use Committee of St. Jude Childrenâ€™s Research Hospital and performed in compliance with relevant policies of the National Institutes of Health and the Animal Welfare Act. All animal challenge experiments were performed in an animal biosafety level 2 containment facilities for the LPAI challenges and in biosafety level 3 enhanced containment laboratories for the HPAI challenges. Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of LPAI A/Mallard/British Columbia/500/2005 (H5N2) in phosphate buffered saline (PBS). Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of HPAI A/Vietnam/1203/2004 (H5N1) in PBS. During LPAI and HPAI infections in ducks, mock infection control groups were also inoculated. Mock infection control birds received an equivalent volume and route of administration with PBS. Animals were monitored daily for clinical signs. : For each ileum and lung tissue, the tissue sample was homogenized in Trizol (Invitrogen Life Technologies), 0.1g tissue/1.0ml Trizol with handheld homogenizer Power Gen125 (Thermo Fisher Scientific). After homogenates were prepared, the samples were centrifuged to separate the liquid phases of Trizol homogenates. 0.5ml of the aqueous phase was passed through a QIagen RNeasy (Qiagen, Valencia, CA) spin column according to manufacturer's instructions for RNA extraction. Samples were frozen and stored at -80 degrees C until all samples were collected for analysis. Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

characteristic: organism part

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_ileum_3_rep1.fastq.gz

text: ArrayExpress

characteristic: broker name

text: mallard

characteristic: infect

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_1_rep1.fastq.gz

text: ArrayExpress

text: mallard

characteristic: common name

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day

characteristic: time


characteristic: ENA-CHECKLIST

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_lung_3_rep2.fastq.gz

text: ArrayExpress

text: mallard

characteristic: description

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


characteristic: ENA-FIRST-PUBLIC

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_ileum_3_rep2.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

characteristic: organism

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day


characteristic: ENA-LAST-UPDATE

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_ileum_3_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day

characteristic: title


characteristic: External Id

text: The Roslin Institute

characteristic: INSDC center name

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_3_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_lung_1_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_3_rep2.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_lung_1_rep2.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

characteristic: organism part

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_1_rep2.fastq.gz

text: ArrayExpress

characteristic: broker name

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_lung_1_rep3.fastq.gz

text: ArrayExpress

text: mallard

characteristic: common name

characteristic: infect

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day


characteristic: ENA-CHECKLIST

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_HP_ileum_3_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day

characteristic: time


characteristic: ENA-FIRST-PUBLIC

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_ileum_3_rep3.fastq.gz

text: ArrayExpress

text: mallard

characteristic: description

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day


characteristic: ENA-LAST-UPDATE

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_LP_lung_1_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

characteristic: organism

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day


characteristic: External Id

text: The Roslin Institute

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_lung_1_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: lung

text: Anas platyrhynchos

text: 1 day

characteristic: title


text: The Roslin Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-2909:Smith_Roslin_APL_con_ileum_3_rep1.fastq.gz

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 3 day


text: The Roslin Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2909:Smith_Roslin_APL_con_ileum_1_rep3.fastq.gz

text: ArrayExpress

text: mallard

text: Protocols: Virus Strains. A/Mallard/British Columbia/500/2005 (H5N2) was used for LPAI challenge. A/Vietnam/1203/2004 (H5N1) was used for HPAI challenge. The viruses were grown in the allantoic cavities of 10-day-old embryonated chicken eggs for 48 hours at 35 degrees C. Allantoic fluid containing virus was harvested and stored at -80 degrees C until use. Virus yield was determined as 50% egg infectious dose (EID50) per millilitre of virus stock by the method of Reed and Muench .Experimental Animals. Domestic Gray Mallards were purchased from Ideal Poultry (Cameron, TX). All experiments involving animals were approved by the Animal Care and Use Committee of St. Jude Childrenâ€™s Research Hospital and performed in compliance with relevant policies of the National Institutes of Health and the Animal Welfare Act. All animal challenge experiments were performed in an animal biosafety level 2 containment facilities for the LPAI challenges and in biosafety level 3 enhanced containment laboratories for the HPAI challenges. Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of LPAI A/Mallard/British Columbia/500/2005 (H5N2) in phosphate buffered saline (PBS). Ducks, except the mock infection control group, were challenged with 106 EID50 intranasally, intratracheally, and intraocularly of HPAI A/Vietnam/1203/2004 (H5N1) in PBS. During LPAI and HPAI infections in ducks, mock infection control groups were also inoculated. Mock infection control birds received an equivalent volume and route of administration with PBS. Animals were monitored daily for clinical signs. : For each ileum and lung tissue, the tissue sample was homogenized in Trizol (Invitrogen Life Technologies), 0.1g tissue/1.0ml Trizol with handheld homogenizer Power Gen125 (Thermo Fisher Scientific). After homogenates were prepared, the samples were centrifuged to separate the liquid phases of Trizol homogenates. 0.5ml of the aqueous phase was passed through a QIagen RNeasy (Qiagen, Valencia, CA) spin column according to manufacturer's instructions for RNA extraction. Samples were frozen and stored at -80 degrees C until all samples were collected for analysis. Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day


text: The Roslin Institute

text: public

characteristic: SRA accession

text: E-MTAB-2909:Smith_Roslin_APL_HP_ileum_1_rep2.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: mallard

text: Anas platyrhynchos

text: Anas platyrhynchos

text: ileum

text: Anas platyrhynchos

text: 1 day


text: Hatching:Long-pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_1-sc-2123597

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_2-sc-2123598

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_3-sc-2123599

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_51_1_phenotypic_4-sc-2123600

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_51_1_phenotypic_5-sc-2123601

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_51_1_phenotypic_6-sc-2123602

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_7-sc-2123603

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_8-sc-2123604

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_9-sc-2123605

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_10-sc-2123606

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_11-sc-2123607

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_51_1_phenotypic_12-sc-2123608

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_51_1_non_phenotypic_1-sc-2123609

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_51_1_non_phenotypic_2-sc-2123610

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_51_1_non_phenotypic_3-sc-2123611

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_4-sc-2123612

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_5-sc-2123613

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_6-sc-2123614

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_7-sc-2123615

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_8-sc-2123616

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_51_1_non_phenotypic_9-sc-2123617

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_51_1_non_phenotypic_10-sc-2123618

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_51_1_non_phenotypic_11-sc-2123619

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Hatching:Long-pec - ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_51_1_non_phenotypic_12-sc-2123620

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 51 clutch 1. A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph51

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_1-sc-2123621

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_2-sc-2123622

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GGTCGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_3-sc-2123623

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GAATCTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_4-sc-2123624

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_5-sc-2123625

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GAGGTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_6-sc-2123626

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCATGGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_52_1_phenotypic_7-sc-2123627

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTTAGCCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_52_1_phenotypic_8-sc-2123628

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTCGCTAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_52_1_phenotypic_9-sc-2123629

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GGAATGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_10-sc-2123630

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GAGCCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_11-sc-2123631

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCTCCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_phenotypic_12-sc-2123632

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTAAGGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_1-sc-2123633

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GAGGATGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_2-sc-2123634

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTTGTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_3-sc-2123635

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GGATTAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZMP_phenotype_52_1_non_phenotypic_4-sc-2123636

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GATAGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZMP_phenotype_52_1_non_phenotypic_5-sc-2123637

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTGTGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: ZMP_phenotype_52_1_non_phenotypic_6-sc-2123638

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCAATCCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_7-sc-2123639

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GACCTTAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_8-sc-2123640

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCCTGTTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_9-sc-2123641

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCACTGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_10-sc-2123642

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GCTAACTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_11-sc-2123643

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GATTCATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

text: public

text: ZMP_phenotype_52_1_non_phenotypic_12-sc-2123644

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 52 clutch 1. A 8 base indexing sequence (GTCTTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph52

text: Danio rerio

text: mixed


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL-EBI)

text: ArrayExpress

characteristic: broker name

text: nervous system cell from episphere

characteristic: cell type

text: Dumeril's clam worm

text: Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18°C, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 μm, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 μl of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 3 IFC-s (termed C3, C4, C5 in the batch information). The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

characteristic: description

text: 48 hpf

characteristic: developmental stage

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

characteristic: organism part

text: Platynereis dumerilii

text: single cell

characteristic: single cell well quality

characteristic: title


text: European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL-EBI)

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: debris


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: no cell


characteristic: External Id

text: public

text: ArrayExpress

text: nervous system cell from episphere

text: Dumeril's clam worm

characteristic: common name

text: 48 hpf

text: Platynereis dumerilii

characteristic: organism

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: multiple cells


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: nervous system cell from episphere

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: single cell


text: public

text: ArrayExpress

text: nervous system cell from episphere

characteristic: cell type

text: Dumeril's clam worm

characteristic: description

text: 48 hpf

characteristic: developmental stage

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: dead cell


text: public

text: ArrayExpress

text: nervous system cell from episphere

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

characteristic: organism part

text: Platynereis dumerilii

text: debris

characteristic: single cell well quality

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: nervous system cell from episphere

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

text: 3 cells


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Roslin Institute

text: public

text: E-MTAB-2910:Smith_Roslin_IBDV_inf_rep3.fq.gz

characteristic: Submitter Id

text: ArrayExpress

text: chicken

text: Protocols: Birds were separated into two experimental rooms, with ad libitum access to food and water. In one room, each bird was mock-infected with 100 ul PBS intra-nasally. In the other room, each bird was infected intra-nasally with 101.3 embryonic infectious dose 50 (EID50) (in 100 µl PBS) of the virulent IBDV strain F52/70. Birds were monitored for clinical signs for 4 dpi and killed at 2, 3 and 4 dpi. Spleen and bursa were collected from all birds. Tissue samples were stabilized in RNAlater (Qiagen, Crawley, UK) and disrupted using a bead mill (Retsch MM 300, Retsch, Haan, Germany) at 20 Hz for 4 min. Total RNA was prepared using a Trizol (Invitrogen, Paisley, UK) extraction method as per the manufacturer's protocol. Samples were resuspended in a final volume of 100 ul of RNAse-free water. Samples were then passed through RNeasy columns (Qiagen) to clean up the RNA further. Concentrations of the samples were calculated by measuring OD260 and OD280 on a spectrophotometer. Quality of the RNA was checked on a bioanalyser (Agilent Technologies, South Queensferry, UK). Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.

text: IBDV

characteristic: infect

text: Gallus gallus

text: Gallus gallus

text: spleen

text: Gallus gallus

text: 4 day

characteristic: time


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Roslin Institute

characteristic: INSDC center name

text: public

text: E-MTAB-2910:Smith_Roslin_IBDV_inf_rep1.fq.gz

text: ArrayExpress

text: chicken

characteristic: common name

text: IBDV

text: Gallus gallus

characteristic: organism

text: Gallus gallus

text: spleen

text: Gallus gallus

text: 4 day


text: The Roslin Institute

characteristic: INSDC first public

text: public

text: E-MTAB-2910:Smith_Roslin_IBDV_con_rep3.fq.gz

text: ArrayExpress

characteristic: broker name

text: chicken

characteristic: description

text: Gallus gallus

text: Gallus gallus

text: spleen

text: Gallus gallus

text: 4 day


text: The Roslin Institute

text: public

text: E-MTAB-2910:Smith_Roslin_IBDV_inf_rep2.fq.gz

text: ArrayExpress

text: chicken

text: IBDV

text: Gallus gallus

text: Gallus gallus

text: spleen

characteristic: organism part

text: Gallus gallus

text: 4 day

characteristic: title


text: The Roslin Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-2910:Smith_Roslin_IBDV_con_rep2.fq.gz

text: ArrayExpress

text: chicken

text: Gallus gallus

text: Gallus gallus

text: spleen

text: Gallus gallus

text: 4 day


characteristic: ENA-CHECKLIST

text: The Roslin Institute

text: public

characteristic: SRA accession

text: E-MTAB-2910:Smith_Roslin_IBDV_con_rep1.fq.gz

text: ArrayExpress

text: chicken

text: Gallus gallus

text: Gallus gallus

text: spleen

text: Gallus gallus

text: 4 day


text: Larval:Day 12 - ZFS:0000039

characteristic: ArrayExpress-DevelopmentalStage

text: Whole larvae

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

characteristic: ArrayExpress-DevelopmentalStage

text: Whole larvae

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped homozygous mutant larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

characteristic: ArrayExpress-DevelopmentalStage

text: Whole larvae

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped heterozygous larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

characteristic: ArrayExpress-DevelopmentalStage

text: Whole larvae

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed


text: Larval:Day 12 - ZFS:0000039

text: Whole larvae

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single 12 dpf genotyped wild type larvae for kdm2aa, allele sa898,ZMP phenotype 111 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph111

text: Danio rerio

text: mixed