characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: C2x83_1Md Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

characteristic: organism

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL-EBI)

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: nervous system cell

characteristic: cell type

text: Dumeril's clam worm

characteristic: common name

text: single cell Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

characteristic: description

text: 48 hpf

characteristic: developmental stage

characteristic: individual

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

characteristic: organism part

text: Platynereis dumerilii

characteristic: title


text: European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL-EBI)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: 2 cells Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

characteristic: organism

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: single cell, debris Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: Dumeril's clam worm

text: no cell observed Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: nervous system cell

text: Dumeril's clam worm

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


text: public

text: ArrayExpress

text: nervous system cell

characteristic: cell type

text: Dumeril's clam worm

characteristic: common name

text: single cell (dead cell) Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

characteristic: description

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: C2x61_1Sb Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

characteristic: developmental stage

characteristic: individual

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

characteristic: organism part

text: Platynereis dumerilii


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: C2x62_1Sb Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: Dumeril's clam worm

text: C2x94_d Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

characteristic: organism

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: C2x55_1L Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: nervous system cell

text: Dumeril's clam worm

text: C2x80_1Mb Protocols: Wild-type P. dumerilii adults were spawned and larvae were incubated in filtered natural seawater, at 18oC, for 47 hours, to reach the desired stage. For cell dissociation, ca 100 47hpf P.dumerilii larvae were picked and incubated for 5 minutes in 0.5% Pronase (Roche cat # 10165921001), 1% sodium thioglycolate (Sigma T0632) diluted in calcium- and magnesium-free artificial sea water. After this treatment, the separated brains (epispheres) were picked, rinsed in 1xPBS, and incubated for 1 minute in 150 ug/ml Liberase (Roche, cat # 05401119001) in 1xPBS. Epispheres were rinsed twice in 1xPBS, transferred to a non-stick eppendorf tube and triturated by pipetting. Dissociated cells were washed twice in 200 ul of 1xPBS and concentrated by centrifuging (1 min, 200xg). Cells were resuspended in 20 ul of 1xPBS, of which 5 ul was loaded on the capture chip (C1 IFC for mRNA-seq for 10-17 um cells, Fluidigm cat # 100-5760). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq (10-17 um, Fluidigm cat # 100-5760) on Fluidigm C1 Single-Cell Auto Prep System. We used SMARTer Ultra Low Input RNA Kit for reverse transcription and the ADVANTAGE-2 PCR kit (Clontech cat # 634832) for PCR. We added 1 ul of 1:4000 dilution of ERCC spike-in RNA (Ambion, cat # 4456740) to the cell lysis mix. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation kit (Illumina cat # FC-131-1096) ). The samples in this study were derived from altogether 5 IFC-s (termed C1, C2, C3, C4, C5 in the batch information). The samples from IFCs C1 and C2 were pooled into one sequencing library. This library was loaded on 2 lanes on Illumina HiSeq2000, obtaining 2 technical replicates per sample. The samples from IFCs C3 and C4 were pooled into one sequencing library. The samples from IFC C5 were pooled into a separate sequencing library.

text: 48 hpf

text: Platynereis dumerilii

text: Platynereis dumerilii

text: episphere

text: Platynereis dumerilii


text: public

tag: Namespace:Institute for molecular and tumor biology Philipps University  Marburg Germany

text: ArrayExpress

characteristic: broker name

text: tumor associated macrophage

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient purification, cells were incubated in Greiner flasks in autologous ascites for 1 h at 37 degrees C in a humidified incubator with 5% CO2 (adherence selection). Nonadherent cells were removed by two PBS washing steps. Using a final concentration of 1% (w/v) formaldehyde for ten minutes at room temperature, proteins and DNA were crosslinked. After adding glycine to a final concentration of 125 mM, the cells were washed twice with ice-cold PBS. After harvesting by scraping and centrifugation, the pellet was resuspended in 1 ml hypotonic ChIP lysis buffer per 8x10^6 cells with protease inhibitors (Sigma) and incubated for 20 minutes. After centrifugation, nuclei were resuspended in ChIP lysis buffer II (RIPA) containing protease inhibitors and sheared with a Branson S250D using a microtip (52 pulses of 1 s, 2 s pause, 20% amplitude) with coolling (ice-ethanol). A 15 min 20,000xg supernatant was used for IP (after preclearing). ChIP was performed as in Adhikary and Mueller, Peroxisome Proliferator-Activated Receptors (PPARs) Methods in Molecular Biology Volume 952, 2013, pp 175-185. DNA was purified using Qiagen Minelute columns. Preceding the PE washing step, the membranes were washed twice with pure methanol in order to remove contaminating DNA-binding lipids that inhibit subsequent low-temperature enzymatic modification steps, which we found to be present in samples from primary macrophages. Libraries were synthesized from 1-2 ng of genomic DNA using the MicroPlex kit (Diagenode, Seraing, Belgium).

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

type: has member


text: Institute for molecular and tumor biology Philipps University Marburg Germany

text: public

text: ArrayExpress

text: tumor associated macrophage

text: human

characteristic: description

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC first public

text: public

text: ArrayExpress

text: tumor associated macrophages

text: ascites ollected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg

characteristic: clinical history

text: human

text: Protocols: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient centrifugation, tumor-associated macrophages (TAMs) were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC last update

text: public

text: ArrayExpress

text: tumor associated macrophages

text: human

text: Protocols: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient centrifugation, tumor-associated macrophages (TAMs) were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Libraries were created using the Illumina Truseq mRNA kit used according to the manufacturer's instructions.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: ArrayExpress

text: human

text: Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. First-strand cDNA samples were prepared from human poly(A)+ RNAs (BD-Clontech) with the SuperScript III kit (Invitrogen). Amplifications were performed in a final volume of 12.5 μL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 μM in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA).

text: Homo sapiens

text: Homo sapiens

text: muscle

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: frozen sample

characteristic: specimen of known storage state


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart

text: Homo sapiens

text: male

text: frozen sample


text: Wellcome Trust Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male

text: frozen sample


text: Wellcome Trust Sanger Institute

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

text: frozen sample


text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: male

text: frozen sample

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: mixed sex

text: frozen sample


text: Wellcome Trust Sanger Institute

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

text: Homo sapiens

text: mixed sex

characteristic: sex

text: frozen sample


characteristic: ENA-LAST-UPDATE

text: Wellcome Trust Sanger Institute

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: mixed sex

text: frozen sample

characteristic: specimen of known storage state


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

text: public

text: frozen sample

characteristic: biosource type

text: ArrayExpress

text: human

text: Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. First-strand cDNA samples were prepared from human poly(A)+ RNAs (BD-Clontech) with the SuperScript III kit (Invitrogen). Amplifications were performed in a final volume of 12.5 Î¼L with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 Î¼M in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA).

text: Homo sapiens

text: Homo sapiens

text: muscle

text: Homo sapiens

text: male


text: Wellcome Trust Sanger Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: heart

text: Homo sapiens

text: male


text: Wellcome Trust Sanger Institute

text: public

characteristic: Submitter Id

text: frozen sample

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male


text: Wellcome Trust Sanger Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: frozen sample

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

text: mixed sex

characteristic: sex


characteristic: External Id

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: mixed sex


text: Wellcome Trust Sanger Institute

text: public

characteristic: INSDC status

text: frozen sample

characteristic: biosource type

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: mixed sex


text: Haemonchus contortus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: SRA accession

text: barber pole worm

text: Haemonchus contortus

characteristic: organism

text: Haemonchus contortus

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (ATCACGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (CGATGTTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTAGGCAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGACCACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (ACAGTGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCCAATGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (CAGATCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (ACTTGATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATCAGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGCTTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGCTACAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (CTTGTACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGGTTGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCGGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAAGCGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCCGTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTACCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCTGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTGCTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGGAGGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGAGCGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGATACGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCATAGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGACTCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCCTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGTGACT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACAGGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCCTCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGGTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGTCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCCATTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGAAGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAACGCTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGGTATG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGAACTGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACTTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCACGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCAGGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAAGTTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCCAGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTATGCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCATTGAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGGCTCAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TATGCCAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCAGATTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACTAGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCAGCTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTCGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAATCTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGGTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCATGGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTTAGCCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTCGCTAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGAATGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGCCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCTCCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTAAGGTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GAGGATGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTTGTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGATTAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed

characteristic: strain


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATAGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCAATCCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

characteristic: sample description

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GACCTTAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCCTGTTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed


text: Hatching:Long-pec- ZFS:0000033

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 80 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GCACTGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/sanger/Zebrafish_Zmpsearch/zmp_ph80

text: Danio rerio

text: mixed