text: Larval:Day 5 - ZFS:0000037

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_48_1_non_phenotypic_11-sc-2177896

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 48 clutch 1. A 8 base indexing sequence (TACTAGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph48

text: Danio rerio

text: mixed


status: PUBLIC

text: Larval:Day 5 - ZFS:0000037

tag: attribute

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zmp_phenotype_48_1_non_phenotypic_12-sc-2177897

characteristic: Submitter Id

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 48 clutch 1. A 8 base indexing sequence (TTCAGCTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph48

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTCGCACC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped homozygous mutant embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TTGTTCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: SRA accession

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (TGCGTGAA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GGTGAGTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Segmentation:1-4 somites - ZFS:0000023

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single genotyped wild type embryo for mitfa, allele W2,ZMP phenotype 151 clutch 1 plus ERCC spike mix 1 (Ambion). A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph151

text: Danio rerio

text: mixed


text: Medical University of Gdansk

text: public

characteristic: INSDC status

tag: Namespace:Medical University of Gdansk

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: amplicon based targeted re-sequencing at minimum of 99x coverage; BRCA MASTR Dx; http://www.multiplicom.com/products/brca-mastr-dx

characteristic: description

text: breast carcinoma

characteristic: disease

text: Copy Number Alterations: 1

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

characteristic: title

type: has member


characteristic: ENA-FIRST-PUBLIC

text: Medical University of Gdansk

text: public

characteristic: SRA accession

text: ArrayExpress

text: human

characteristic: common name

text: breast carcinoma

text: Copy Number Alterations: 253

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical University of Gdansk

text: public

text: ArrayExpress

text: human

text: breast carcinoma

text: Copy Number Alterations: 56

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


text: Medical University of Gdansk

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: breast carcinoma

text: Copy Number Alterations: 67

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


text: Medical University of Gdansk

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: breast carcinoma

text: Copy Number Alterations: 8

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


text: Medical University of Gdansk

text: public

text: ArrayExpress

text: human

characteristic: description

text: breast carcinoma

characteristic: disease

text: Copy Number Alterations: 359

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


text: Larval:Day 6 - ZFS:0000038

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_42_1_phenotypic_1-sc-2178018

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGTCTATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_42_1_non_phenotypic_1-sc-2178019

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TATGTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: zmp_phenotype_42_1_phenotypic_2-sc-2178020

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TTACTCGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_2-sc-2178021

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TCGTTAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_42_1_phenotypic_3-sc-2178022

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TACCGAGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_3-sc-2178023

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGTTCTCC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_4-sc-2178024

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TTGCGTAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_42_1_phenotypic_5-sc-2178025

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TCTACGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_5-sc-2178026

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGACAGAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zmp_phenotype_42_1_phenotypic_6-sc-2178027

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TAGAACAC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zmp_phenotype_42_1_non_phenotypic_6-sc-2178028

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TCATCCTA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: zmp_phenotype_42_1_phenotypic_7-sc-2178029

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGCTGATA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_7-sc-2178030

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TAGACGGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_42_1_phenotypic_8-sc-2178031

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGTGAAGA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_8-sc-2178032

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TCTCTTCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_9-sc-2178033

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TGAAGCCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_42_1_phenotypic_10-sc-2178034

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (TACCACCA) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_42_1_non_phenotypic_11-sc-2178035

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (GATCTCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zmp_phenotype_42_1_phenotypic_12-sc-2178036

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (GTGTCCTT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Day 6 - ZFS:0000038

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zmp_phenotype_42_1_non_phenotypic_12-sc-2178037

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 42 clutch 1. A 8 base indexing sequence (GACGGATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph42

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: zmp_phenotype_54_1_phenotypic_1-sc-2178038

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCAACATT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_2-sc-2178039

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GGTCGTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_3-sc-2178040

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GAATCTGT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_4-sc-2178041

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTACATCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_5-sc-2178042

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GAGGTGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_54_1_phenotypic_6-sc-2178043

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCATGGCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_7-sc-2178044

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTTAGCCT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zmp_phenotype_54_1_phenotypic_8-sc-2178045

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTCGCTAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zmp_phenotype_54_1_phenotypic_9-sc-2178046

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GGAATGAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: zmp_phenotype_54_1_phenotypic_10-sc-2178047

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GAGCCAAT) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_phenotypic_11-sc-2178048

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically abnormal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCTCCTTG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_1-sc-2178049

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GAGGATGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_2-sc-2178050

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTTGTCGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_3-sc-2178051

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GGATTAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

text: SC

text: public

characteristic: SRA accession

text: zmp_phenotype_54_1_non_phenotypic_4-sc-2178052

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GATAGAGG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_5-sc-2178053

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTGTGTCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zmp_phenotype_54_1_non_phenotypic_6-sc-2178054

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCAATCCG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: zmp_phenotype_54_1_non_phenotypic_7-sc-2178055

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GACCTTAG) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

characteristic: INSDC status

text: zmp_phenotype_54_1_non_phenotypic_8-sc-2178056

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCCTGTTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_9-sc-2178057

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCACTGTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_10-sc-2178058

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GCTAACTC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_11-sc-2178059

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GATTCATC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: Larval:Protruding-mouth - ZFS:0000035

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: zmp_phenotype_54_1_non_phenotypic_12-sc-2178060

text: zebrafish

text: Danio rerio

text: 3' end enriched mRNA from a single phenotypically normal embryo from ZMP phenotype 54 clutch 1. A 8 base indexing sequence (GTCTTGGC) is bases 13 to 20 of read 1 followed by CG and polyT. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph54

text: Danio rerio

text: mixed


characteristic: ENA-LAST-UPDATE

text: public

characteristic: SRA accession

tag: Namespace:Institute for molecular and tumor biology Philipps University  Marburg Germany

text: ArrayExpress

characteristic: broker name

text: macrophage

characteristic: cell type

text: human

text: Protocols: Cells were isolated on October 14th 2014 from two samples of healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 °C, 5 % CO2 in 2ml RPMI medium supplemented with 10 % FCS (both PAA). Lysis in hypotonic lysis buffer Nuclei were resuspended in ChIP RIPA and sheared with a Branson S250D using a microtip (60 pulses of 1 s, 2 s pause, 20% amplitude) with coolling (ice-ethanol). A 15 min 20,000xg supernatant was used for IP (after preclearing). The Diagenode Microplex Library Preparation kit (Diagenode, C05010011) was used according to the manufacturer's instructions with 1-2 ng of input DNA. DNA concentrations were measured on a Nanodrop 3300 using the Quant-It dsDNA High-Sensitivity Assay Kit (Life Technologies).

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: External Id

text: Institute for molecular and tumor biology Philipps University Marburg Germany

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: macrophage

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

text: macrophage

text: human

text: Protocols: Cells were isolated on June 06th 2013 from healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 6d in humidified incubator at 37 °C, 5 % CO2 in 2ml RPMI medium supplemented with 10 % FCS (both PAA). Cells were treated with control (DMSO), L165 1 uM final concentration or ST247 300 nM final concentration for 1d. Cells were lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Libraries were created using the Illumina Truseq mRNA kit used according to the manufacturer's instructions.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

text: ArrayExpress

text: macrophage

text: human

text: Protocols: Cells were isolated on June 17th 2014 from healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 oC, 5 % CO2 in 2ml X-VIVO 10 (Biozym scientific, 881022), medium. Cells were treated with control (DMSO), L165 1 uM final concentration or ST247 300 nM final concentration for 1d. Cells were lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: UNIV-WUE

text: public

text: GATCTACGGATCGTGT

characteristic: barcodesequence

characteristic: collection date

text: bramble

text: RubusXflower

text: tropical island

characteristic: environment (biome)

text: island

characteristic: environment (feature)

text: flower

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Rubus

text: plant-associated

characteristic: plant-associated environmental package

text: Metrosideros Microbiota

characteristic: project name

text: Rubus

text: MiSeq

characteristic: sequencing method

text: Metrosideros associated


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIV-WUE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: TAGCAGCTGATCGTGT

text: evening primroses

text: OenotheraXflower

characteristic: description

text: tropical island

text: island

text: flower

text: USA

text: mimarks-survey

text: Oenothera

text: plant-associated

text: Metrosideros Microbiota

text: Oenothera

text: MiSeq

text: Metrosideros associated

characteristic: title


text: UNIV-WUE

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: TCTCTATGGATCGTGT

characteristic: barcodesequence

characteristic: collection date

text: bramble

characteristic: common name

text: RubusXleaf

text: tropical island

characteristic: environment (biome)

text: island

characteristic: environment (feature)

text: leaves

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: mimarks-survey

text: Rubus

characteristic: organism

text: plant-associated

text: Metrosideros Microbiota

text: Rubus

text: MiSeq

text: Metrosideros associated


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIV-WUE

text: public

text: GTAACGAGGATCGTGT

text: evening primroses

text: OenotheraXleaf

text: tropical island

text: island

text: leaves

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Oenothera

text: plant-associated

characteristic: plant-associated environmental package

text: Metrosideros Microbiota

characteristic: project name

text: Oenothera

text: MiSeq

characteristic: sequencing method

text: Metrosideros associated


text: Macrophage

characteristic: ArrayExpress-CellType

text: Poly I:C

characteristic: ArrayExpress-GrowthCondition

text: mus musculus

characteristic: ArrayExpress-Species

text: passage 4

characteristic: ArrayExpress-TimePoint

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Screen 6 TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

characteristic: sample description

text: Mus musculus


text: Macrophage

text: LPS

text: mus musculus

text: passage 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Screen 6 TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

characteristic: ArrayExpress-CellType

text: M TRI-dap (NOD agonist)

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: Macrophage

text: ODN 1826 (CpG)

characteristic: ArrayExpress-GrowthCondition

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: Macrophage

text: Control 4hr

text: mus musculus

characteristic: ArrayExpress-Species

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: Macrophage

text: Salmonella

text: mus musculus

text: passage 4

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 TMEM-165 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: Macrophage

text: Poly I:C

text: mus musculus

text: passage 5

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: LPS

text: mus musculus

text: passage 5

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: M TRI-dap (NOD agonist)

text: mus musculus

text: passage 5

text: SC

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: Macrophage

text: ODN 1826 (CpG)

text: mus musculus

text: passage 5

text: SC

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: Macrophage

text: Control 4hr

text: mus musculus

text: passage 5

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: Macrophage

text: Salmonella

text: mus musculus

text: passage 5

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: Macrophage

text: Influenza 6hr

text: mus musculus

text: passage 5

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

characteristic: sample description

text: Mus musculus


text: Macrophage

text: Influenza 24hr

text: mus musculus

text: passage 5

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: Macrophage

text: Control 24hr

text: mus musculus

text: passage 5

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated MAPK14 Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: Macrophage

text: Poly I:C

text: mus musculus

text: passage 4

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: LPS

text: mus musculus

text: passage 4

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: M TRI-dap (NOD agonist)

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: Macrophage

text: ODN 1826 (CpG)

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: Macrophage

characteristic: ArrayExpress-CellType

text: Control 4hr

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: Macrophage

text: Salmonella

characteristic: ArrayExpress-GrowthCondition

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: Macrophage

text: Influenza 6hr

text: mus musculus

characteristic: ArrayExpress-Species

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: Macrophage

text: Influenza 24hr

text: mus musculus

text: passage 4

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: Macrophage

text: Control 24hr

text: mus musculus

text: passage 4

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Plekhm2 Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: Macrophage

text: Poly I:C

text: mus musculus

text: passage 4

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: LPS

text: mus musculus

text: passage 4

text: SC

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: M TRI-dap (NOD agonist)

text: mus musculus

text: passage 4

text: SC

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: Macrophage

text: ODN 1826 (CpG)

text: mus musculus

text: passage 4

text: SC

text: public

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: Macrophage

text: Control 4hr

text: mus musculus

text: passage 4

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: Macrophage

text: Salmonella

text: mus musculus

text: passage 4

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

characteristic: sample description

text: Mus musculus


text: Macrophage

text: Influenza 6hr

text: mus musculus

text: passage 4

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: Macrophage

text: Influenza 24hr

text: mus musculus

text: passage 4

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: Macrophage

text: Control 24hr

text: mus musculus

text: passage 4

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Lmnb2 Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: Macrophage

text: Poly I:C

text: mus musculus

text: passage 27

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Poly I:C (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: LPS

text: mus musculus

text: passage 27

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with LPS (Toll-like receptor agonist)

text: Mus musculus


text: Macrophage

text: M TRI-dap (NOD agonist)

text: mus musculus

text: passage 27

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with M TRI-dap (NOD agonist)

text: Mus musculus


text: Macrophage

characteristic: ArrayExpress-CellType

text: ODN 1826 (CpG)

text: mus musculus

text: passage 27

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with ODN 1826 (CpG)

text: Mus musculus


text: Macrophage

text: Control 4hr

characteristic: ArrayExpress-GrowthCondition

text: mus musculus

text: passage 27

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Wild Type Knock-out Embryonic Stem Cell Derived Macrophages 4hr

text: Mus musculus


text: Macrophage

text: Salmonella

text: mus musculus

characteristic: ArrayExpress-Species

text: passage 27

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Salmonella

text: Mus musculus


text: Macrophage

text: Influenza 6hr

text: mus musculus

text: passage 27

characteristic: ArrayExpress-TimePoint

text: SC

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 6 hr

text: Mus musculus


text: Macrophage

text: Influenza 24hr

text: mus musculus

text: passage 27

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Wild Type Knock-out Embryonic Stem Cell Derived Macrophages stimulated with Influenza 24 hr

text: Mus musculus


text: Macrophage

text: Control 24hr

text: mus musculus

text: passage 27

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Screen 6 Unstimulated Wild Type Knock-out Embryonic Stem Cell Derived Macrophages 24hr

text: Mus musculus


text: CeMM - Center for Molecular Medicine

characteristic: INSDC last update

text: public

tag: Namespace:CeMM - Center for Molecular Medicine

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: HEK293 and K562 cells were grown in DMEM and IMDM, respectively and supplemented with 10% fetal bovine serum. RNA was extracted using TRI reagent (SIGMA), then it was DNAse I treated (DNA free kit from Ambion), then it was treated with RiboZero kit (Epicentre) to remove ribosomal RNA. The library was prepared using ScriptSeq kit version 1 (Epicentre).

characteristic: description

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


E-MTAB-3120:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3120:Sample 2

tag: Namespace:Experimental Radiation Oncology, MD Anderson Cancer Center

characteristic: Submitter Id

text: ArrayExpress

text: kidney cell

characteristic: cell type

text: human

text: Protocols: Immortalized human embryonic kidney cell line HEK293T cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin. ChIP-seq, Read length 75nt PE, KAPA Library Prep Kit, Number of PCR cycles 0 cycles, Amount of DNA used to make to libraries: ~20ng, 4 samples were multiplexed per lane.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 2


E-MTAB-3120:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Experimental Radiation Oncology, MD Anderson Cancer Center

text: public

text: E-MTAB-3120:Sample 4

text: ArrayExpress

text: kidney cell

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 4


E-MTAB-3120:Sample 1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-3120:Sample 1

text: ArrayExpress

text: kidney cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 1


E-MTAB-3120:Sample 3

characteristic: INSDC last update

text: public

text: E-MTAB-3120:Sample 3

text: ArrayExpress

characteristic: broker name

text: kidney cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 3


text: Wellcome Trust Sanger Institute

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: frozen sample

characteristic: biosource type

text: ArrayExpress

text: human

text: Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. First-strand cDNA samples were prepared from human poly(A)+ RNAs (BD-Clontech) with the SuperScript III kit (Invitrogen). Amplifications were performed in a final volume of 12.5 μL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 μM in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA).

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: muscle

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: heart

text: Homo sapiens

text: male

characteristic: title


text: Wellcome Trust Sanger Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: testis

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: mixed sex


text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: frozen sample

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens

text: male


text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

text: frozen sample

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: mixed sex


text: Wellcome Trust Sanger Institute

text: public

text: frozen sample

characteristic: biosource type

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: mixed sex


E-MTAB-2636:Spoc1 ChIP-seq

text: public

text: E-MTAB-2636:Spoc1 ChIP-seq

tag: Namespace:Max Planck Institute for molecular Genetics OWL Epigenomics

text: ArrayExpress

text: ES-E14 cell

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: E14 ES cells were grown in the presence of LIF and 20% FBS. The cells were collected and cross-linked for 15 minutes with 1% formaldehyde. The fixation reaction was quenched by the addition of glycine (0.125M) for 10 minutes. The cells were then lysed on ice for 10 minutes in 1.3 ml of ChIP buffer, 1 volume of SDS buffer (100mM NaCl, 50 mM Tris-HCl pH8, 5mM EDTA, 0.2% NaN3 and 0.5% SDS) : 0.5 volume of Triton dilution buffer (100mM Tris-HCl pH8.6, 100 mM NaCl, 5 mM EDTA, 0.2% NaN3 and 5% Triton-X 100). The lysate was passed through a syringe multiple times and then sonicated in Bioruptor at high frequency for 2 x 6 cycles. The lysate was then centrifuged at 13,000 rpm for 30 minutes to pellet debris and a protein determination was measured. 1 mg of chromatin was then incubated with 5 µl of SPOC1 antibody (CR56) or rabbit IgG control antibody overnight at 4°C under rotation. 30 µl of protein A beads were then added to the reaction and allowed to incubate at 4°C for 2h. The beads were then washed by successive low salt (0.1% SDS, 1% Triton X, 2 mM EDTA, 20 mM Tris-HCl and 150 mM NaCl), high salt (0.1% SDS, 1% Triton X, 2 mM EDTA, 20 mM Tris-HCl and 500 mM NaCl) and LiCl washes and then eluted for 3h at 65°C with 110 µl of elution buffer (1% SDS and 100 mM NaHCO3). The RNA and proteins were then digested with RNAse A and Proteinase K and the DNA purified and sequenced Library construction;according to manufacturers protocols

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: Spoc1 ChIP-seq


E-MTAB-2636:IgG Control

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Max Planck Institute for molecular Genetics OWL Epigenomics

text: public

text: E-MTAB-2636:IgG Control

text: ArrayExpress

text: ES-E14 cell

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: male

text: IgG Control

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Biochemistry University of Cambridge

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Biochemistry University of Cambridge

text: public

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Control


text: Danio rerio

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC last update

text: public

text: zebrafish

text: Danio rerio

text: DNA libraries made by the addition of tailed oligos in a second round of PCR.

characteristic: sample description

text: Danio rerio


text: 28 days

characteristic: ArrayExpress-Age

text: Liver

characteristic: ArrayExpress-CellType

text: Ctrl

characteristic: ArrayExpress-Compound

text: wild type

characteristic: ArrayExpress-Genotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: Ctrl 2cells

text: Mus musculus

characteristic: strain


text: 28 days

text: Liver

text: Ctrl

text: wild type

text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Ctrl 1 cell

text: Mus musculus


text: 28 days

text: Liver

text: Ctrl

text: wild type

text: male

text: Mus musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Ctrl 10 cells

text: Mus musculus


text: 28 days

characteristic: ArrayExpress-Age

text: Liver

text: DEN

text: wild type

text: male

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: DEN 2 cells

characteristic: sample description

text: Mus musculus


text: 28 days

text: Liver

characteristic: ArrayExpress-CellType

text: DEN

characteristic: ArrayExpress-Compound

text: wild type

characteristic: ArrayExpress-Genotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: DEN 1 cell

text: Mus musculus


text: 28 days

text: Liver

text: DEN

text: wild type

text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: DEN 10 cells

text: Mus musculus

characteristic: strain


text: Homo Sapien

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


CAAK

text: EBI

characteristic: INSDC last update

text: public

text: CAAK

text: ENA

characteristic: broker name

text: zebrafish

characteristic: common name

text: Danio rerio

characteristic: organism

text: Danio rerio

text: Tuebingen

text: BioSample entry for WGS set CAAK

characteristic: title


CCAZ

text: EBI

text: public

text: CCAZ

text: ENA

text: cat scratch disease bacillus

text: Afipia felis

text: Afipia felis

text: BioSample entry for WGS set CCAZ


CBYH

text: EBI

text: public

text: CBYH

text: ENA

text: wild goat

text: Capra aegagrus

text: Capra aegagrus

text: BioSample entry for WGS set CBYH


CBYI

text: EBI

text: public

text: CBYI

text: ENA

text: mouflon

text: Ovis aries musimon

text: Ovis aries musimon

text: BioSample entry for WGS set CBYI


CAJV

text: EBI

text: public

text: CAJV

text: ENA

text: domesticated barley

text: Barke

characteristic: cultivar

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

text: vulgare

characteristic: sub species

text: BioSample entry for WGS set CAJV


CBLZ

characteristic: ENA-CHECKLIST

text: EBI

text: public

text: CBLZ

text: ENA

text: domesticated barley

text: Morex

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare

text: BioSample entry for WGS set CBLZ


CBUC

characteristic: ENA-FIRST-PUBLIC

text: EBI

text: public

text: CBUC

text: ENA

text: bread wheat

text: Chinese Spring

text: Triticum aestivum

text: Triticum aestivum

text: BioSample entry for WGS set CBUC


CAWD

text: EBI

text: public

characteristic: INSDC status

text: CAWD

text: ENA

text: dog heartworm nematode

text: Dirofilaria immitis

text: Dirofilaria immitis

text: BioSample entry for WGS set CAWD


CABZ

text: EBI

text: public

characteristic: SRA accession

text: CABZ

text: ENA

text: zebrafish

text: Danio rerio

text: Danio rerio

text: Tuebingen

text: BioSample entry for WGS set CABZ


CCAF

text: EBI

text: public

text: CCAF

text: ENA

text: rainbow trout

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss

text: BioSample entry for WGS set CCAF


text: London Research Institute CRUK

text: public

text: E-MTAB-3125:Ring1aKO_nuclear_RNA_seq_rep1

tag: Namespace:London Research Institute CRUK

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: J1 (129S4/SvJae) and 129SvJae/Cast WT mESCs were grown under standard ESC culture conditions. Ring1A−/−;Ring1Bfl/fl;Rosa26::CreERT2 mESCs (Ring1A-KO) were cultured as described previously in Endoh et al. Dishes were coated with 0.1 % gelatine and irradiated mouse embryonic fibroblasts (MEFs). For harvesting, ESCs were trypsinized and pre-plated twice for 30 min to remove contaminating feeder cells. Mouse ESCs were washed in PBS and approximately 30-50 x 106 ES cells were lysed for 5 min in 0.5 ml cold buffer RLN (50 mM TrisHCl pH 7.5, 140 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 0.4% Igepal). Nuclei were pelleted by spinning at 300g for 10 min at 4°C. Nuclear RNA was isolated using TRIsure (Bioline), treated with DNaseI (Roche) and re-purified using an RNeasy Mini Kit (Qiagen). Strand-specific RNA-seq libraries were prepared as described previously by marking the second strand with dUTP but with some modifications8,9. 250 ng nuclear RNA was fragmented using a Covaris E220 instrument at standard RNA settings for 60 sec. Fragmented RNA was precipitated and first strand synthesis was carried out using SuperScript III (Invitrogen) with 4 µg of actinomycin D (Sigma). Nucleotides were removed with mini quick spin DNA columns (Roche) and second strand synthesis was performed using E.coli DNA ligase (NEB), DNA Polymerase I (NEB) and RNase H (Fermentas), replacing dTTP with dUTP (Fermentas). Following purification on QIAquick columns (Qiagen), TruSeq Illumina adapters were ligated with T4 DNA Ligase, (Enzymatics). Libraries were purified on QIAquick columns, treated with USER (NEB) to destroy the second strand and size selected using AMPure XP beads. Libraries were amplified with 9-11 PCR cycles.

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Ring1a knockout

characteristic: phenotype

text: Mus musculus

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: London Research Institute CRUK

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-3125:Ring1a/b_dKO_nuclear_RNA_seq_rep1

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Protocols: J1 (129S4/SvJae) and 129SvJae/Cast WT mESCs were grown under standard ESC culture conditions. Ring1A−/−;Ring1Bfl/fl;Rosa26::CreERT2 mESCs (Ring1A-KO) were cultured as described previously in Endoh et al. Dishes were coated with 0.1 % gelatine and irradiated mouse embryonic fibroblasts (MEFs). For harvesting, ESCs were trypsinized and pre-plated twice for 30 min to remove contaminating feeder cells. Conditional deletion of Ring1B in Ring1A-KO cells was carried out by addition of 800 nM tamoxifen for 48 h as previously described in Endoh et al. Mouse ESCs were washed in PBS and approximately 30-50 x 106 ES cells were lysed for 5 min in 0.5 ml cold buffer RLN (50 mM TrisHCl pH 7.5, 140 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 0.4% Igepal). Nuclei were pelleted by spinning at 300g for 10 min at 4°C. Nuclear RNA was isolated using TRIsure (Bioline), treated with DNaseI (Roche) and re-purified using an RNeasy Mini Kit (Qiagen). Strand-specific RNA-seq libraries were prepared as described previously by marking the second strand with dUTP but with some modifications8,9. 250 ng nuclear RNA was fragmented using a Covaris E220 instrument at standard RNA settings for 60 sec. Fragmented RNA was precipitated and first strand synthesis was carried out using SuperScript III (Invitrogen) with 4 µg of actinomycin D (Sigma). Nucleotides were removed with mini quick spin DNA columns (Roche) and second strand synthesis was performed using E.coli DNA ligase (NEB), DNA Polymerase I (NEB) and RNase H (Fermentas), replacing dTTP with dUTP (Fermentas). Following purification on QIAquick columns (Qiagen), TruSeq Illumina adapters were ligated with T4 DNA Ligase, (Enzymatics). Libraries were purified on QIAquick columns, treated with USER (NEB) to destroy the second strand and size selected using AMPure XP beads. Libraries were amplified with 9-11 PCR cycles.

text: Mus musculus

text: Mus musculus

text: tamoxifen-mediated knockout of Ring1b on Ring1a-/- background

text: Mus musculus


text: London Research Institute CRUK

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: house mouse

characteristic: common name

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: London Research Institute CRUK

text: public

characteristic: INSDC status

text: E-MTAB-3125:Ring1a/b_dKO_nuclear_RNA_seq_rep2

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus


text: London Research Institute CRUK

text: public

characteristic: SRA accession

text: E-MTAB-3125:Ring1aKO_nuclear_RNA_seq_rep2

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: Ring1a knockout

text: Mus musculus


status: KILLED

text: male

characteristic: ArrayExpress-Sex

text: Homo Sapien

characteristic: ArrayExpress-Species

text: SC

text: killed

characteristic: Sample Description

characteristic: Strain

text: human

text: Homo sapiens

text: Homo sapiens


text: Mus musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


E-MTAB-3137:FOXM1 2

text: Pfizer Neusentis

characteristic: INSDC last update

text: public

text: E-MTAB-3137:FOXM1 2

tag: Namespace:Pfizer Neusentis

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: common name

text: Protocols: RPE were generated from the hESC cell line SHEF1.3 or iPSC reprogrammed from erythroblasts using the spontaneous differentiation method described previously (Vugler et al., 2008). Pluripotent cells were cultured as feeder-free colonies on hESC-qualified Matrigel (BD) in mTesR1 (StemCell Technologies) media. RPE foci were excised with a scalpel and dissociated into a single cell suspension using Accutase (Gibco) and plated onto CellStart (Invitrogen) coated surfaces. ChIP was performed essentially as described before (Sanders et al., 2013). Briefly, 1 million cells were crosslinked with 1% Formaldehyde for 10 minutes and the reaction was quenched with 125mM glycine. Sonication was performed on isolated nuclei using the Bioruptor Plus with cooling system for 35 cycles of each 30 seconds on and 30 seconds off at the maximum setting leading to a fragment size between 200-300bp. For each IP, 50 ul of Protein A Dynabeads were incubated with 5 ug of the appropriate antibody (anti-FOXM1 [Santa Cruz Biotechnology, SC-502] or Rabbit IgG REF) overnight at 4oC. Chromatin immunoprecipitation was performed by adding the 100 ul of preblocked antibody-bead complexes per sample and incubating overnight at 4oC on a rotator. Elution and crosslink reversal of both sample and input was performed by incubating the samples for 6 hours at 65oC in a waterbath. The DNA was purified using the Zymo ChIP DNA Clean & Concentrator kit (Zymo Research) according to the manufacturer's instructions. The sequencing library was generated using the MicroPlex Library Preparation kit (Diagenode) and size selected using Agencourt AMPure XP. The libraries were quantified using the qPCR based Illumina library quantification kit (Kappa Biosystems) .

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: FOXM1 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Pfizer Neusentis

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: IgG

characteristic: title


E-MTAB-3137:FOXM1 1

text: Pfizer Neusentis

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-3137:FOXM1 1

text: ArrayExpress

text: epithelial cell

characteristic: cell type

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: FOXM1 1


text: Pfizer Neusentis

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Input


text: Danio rerio

characteristic: ArrayExpress-Species

text: SC

text: public

text: zebrafish

text: Danio rerio

characteristic: sample description

text: Danio rerio


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EBI

text: killed

text: ENA

text: barber pole worm

text: Haemonchus contortus

text: Haemonchus contortus

text: ISE/inbred ISE

characteristic: strain

text: BioSample entry for WGS set CAVP