text: ESC

characteristic: ArrayExpress-CellType

text: embryonic stem cell line

characteristic: ArrayExpress-DevelopmentalStage

text: alternative 2i/LIF

text: male

text: Mus Musculus

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mES_ai2_s2_cell94-sc-2013-09-25T11:29:33Z-1706483

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


status: PUBLIC

text: ESC

tag: attribute

text: embryonic stem cell line

characteristic: ArrayExpress-Genotype

text: alternative 2i/LIF

characteristic: ArrayExpress-GrowthCondition

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: mES_ai2_s2_cell95-sc-2013-09-25T11:29:37Z-1706484

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL/6Ncr male x 129S6/SvEvTac female

characteristic: sample description

text: Mus musculus

characteristic: strain


text: ESC

characteristic: ArrayExpress-CellType

text: embryonic stem cell line

characteristic: ArrayExpress-DevelopmentalStage

text: G4 ES cell line from a male blastocyst from a C57BL/6Ncr male x 129S6/SvEvTac female

text: alternative 2i/LIF

text: male

text: Mus Musculus

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mES_ai2_s2_cell96-sc-2013-09-25T14:08:06Z-1706485

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_transfected_rep3-sc-2013-09-27T10:26:06Z-1710009

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: gRNA_site_1_against_26_GPI_genes_control_rep3-sc-2013-09-27T10:26:14Z-1710010

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_control_rep3-sc-2013-09-27T10:26:21Z-1710011

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: gRNA_site_1_against_26_GPI_genes_transfected_rep4-sc-2013-09-27T10:26:28Z-1710012

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

characteristic: SRA accession

text: gRNA_site_2_against_26_GPI_genes_transfected_rep4-sc-2013-09-27T10:26:36Z-1710013

text: house mouse

characteristic: common name

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_1_against_26_GPI_genes_transfected_rep3-sc-2013-09-27T10:27:14Z-1710008

text: house mouse

text: Mus musculus

characteristic: organism

text: PCR products

characteristic: sample description

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_1_against_26_GPI_genes_control_rep4-sc-2013-09-27T10:27:21Z-1710014

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: gRNA_site_2_against_26_GPI_genes_control_rep4-sc-2013-09-27T10:27:26Z-1710015

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone2_NAG_Indel-sc-2013-09-27T10:27:33Z-1710017

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone3_NAG_Indel-sc-2013-09-27T10:53:35Z-1710018

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone4_NAG_Indel-sc-2013-09-27T10:53:42Z-1710019

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone1_NAG_Indel-sc-2013-09-27T10:56:33Z-1710016

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone10_NAG_Indel-sc-2013-09-27T10:56:41Z-1710025

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone1_NAG_Indel-sc-2013-09-27T10:56:48Z-1710026

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone5_NAG_Indel-sc-2013-09-27T10:56:56Z-1710020

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone6_NAG_Indel-sc-2013-09-27T10:57:05Z-1710021

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone7_NAG_Indel-sc-2013-09-27T10:57:11Z-1710022

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone8_NAG_Indel-sc-2013-09-27T11:00:48Z-1710023

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_clone9_NAG_Indel-sc-2013-09-27T11:00:57Z-1710024

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone8_NAG_Indel-sc-2013-09-27T11:01:03Z-1710033

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone2_NAG_Indel-sc-2013-09-27T11:01:11Z-1710027

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone3_NAG_Indel-sc-2013-09-27T11:01:18Z-1710028

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone4_NAG_Indel-sc-2013-09-27T11:01:24Z-1710029

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone5_NAG_Indel-sc-2013-09-27T11:01:30Z-1710030

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone6_NAG_Indel-sc-2013-09-27T11:01:35Z-1710031

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone7_NAG_Indel-sc-2013-09-27T11:01:41Z-1710032

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone9_NAG_Indel-sc-2013-09-27T11:01:47Z-1710034

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_8_clone10_NAG_Indel-sc-2013-09-27T11:01:53Z-1710035

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_NAG_Indel-sc-2013-09-27T11:01:59Z-1710036

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_PigA3_transient_tf1-sc-2013-09-27T11:02:04Z-1710037

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_PigA3_transient_tf2-sc-2013-09-27T11:02:10Z-1710038

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_PigA3_transient_cntl1-sc-2013-09-27T11:02:15Z-1710039

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus musculus

text: SC

text: public

text: JM8_5_PigA3_transient_cntl2-sc-2013-09-27T11:02:21Z-1710040

text: house mouse

text: Mus musculus

text: PCR products

text: Mus musculus


text: male

text: Mus Musculus

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA extracted from mESC

text: Mus musculus


E-MTAB-1117:adult head repo FACS 2

text: EMBL-EBI

text: public

text: E-MTAB-1117:adult head repo FACS 2

text: 2 day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Flies were kept on standard media at 25C. 1-3 days old flies were collected and frozen in liquid nitrogen at the same time of the day. Frozen flies were stored at -80C until used for chromatin preparation. The following strains were used for ChIP experiments: 2202U2 (wild-type), elav-GAL4 (Bloomington stock no. 458), repo-GAL4 (Sepp, 2001), take-out-GAL4 (Dauwalder, 2002), UAS-EGFP-RPB3 (Yao et al., 2006), UAS-H2Av-GFP, gH2Av-GFP. Details of generating the latter two strains is available upon request. Fly strains used for sequencing experiments were backcrossed to 2202U2 at least for 4-6 generations. Heads of frozen flies were separated using 630 um and 400 um sieves. 400-600 fly heads were homogenized in homogenization buffer (HB) at 4C (HB: 350 mM sucrose, 15 mM HEPES pH 7.6, 10 mM KCl, 5 mM MgCl2, 0.5 mM EGTA, 0.1 mM EDTA, 0.1% Tween, freshly completed with 1mM DTT and Protease Inhibitor Cocktail (PIC)(Roche)). The homogenate was fixed using 1% formaldehyde for 10 minutes at room temperature. The tissue debris was filtered with 60 um nylon net (Millipore). Nuclei were collected and washed with RIPA buffer at 4C (RIPA: 150 mM NaCl, 25 mM HEPES pH 7.6, 1 mM EDTA, 1% Triton-X, 0.1% SDS, 0.1% DOC, freshly completed with PIC). For the fragmentation of chromatin a 2 step sonication was used: Branson250 (7 cycles, intensity 5, pulsing 16 sec) and Covaris sonicator (PIP175, DC20, CB20, time 4 min). After sonication debris was collected and chromatin was stored at -80C. Fragment size was checked after cross-link reversal on agarose gel. 10-15 ug chromatin was used per IP. Dynabeads protein G (Invitrogen) were equilibrated in RIPA with 1 ug/ul salmon sperm DNA and 1 ug/ul BSA. Chromatin was always pre-absorbed with beads without antibody. The cleared chromatin was incubated with antibodies overnight. The following antibodies were used for ChIP in this study: anti-GFP (goat, Ladurner lab stock), anti- RPB3 ((Adelman et al., 2006) and Ladurner lab stock), anti-RPB1 (7G5, Euromedex) anti-H2Av and anti-H2A (Leach, 2000), anti-H3 (abcam 1791). After IP, beads were washed 4-times with RIPA and once with LiCl wash buffer (250 mM LiCl, 10 mM Tris-Hcl pH 8.0, 1 mM EDTA, 0.5% NP-40, 0.5% DOC, freshly added PIC). Beads were resuspended in TE buffer and incubated overnight at 65 C. RNA was degraded using RNase A (Fermentas) 30 min at 37 C and proteins were digested with protease K (10 mg/ml) at 55C for 1.5 hours. Immunoprecipitated DNA was purified using Qiagen MiniElute columns and 3-10 ng DNA was sent for sequencing. In some cases, more technical replicates had to be pooled in order to obtain the required amounts. ChIP on embryos was carried as described previously (Sandmann et al., 2007). Briefly, 0-6 hours embryos were collected and washed with PBST 3% Triton-X, dechorionated with 3% Na Hypochlorite, fixed with formaldehyde and heptane. After quenching the fixing, the embryos were washed, frozen in liquid nitrogen and stored at -80C. Chromatin preparation from fixed, frozen embryos was continued with homogenization as described above.

characteristic: description

text: adult

characteristic: developmental stage

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head (glia)

characteristic: organism part

text: Drosophila melanogaster

characteristic: strain

text: adult head repo FACS 2

characteristic: title


E-MTAB-1117:adult head elav FACS 2

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-1117:adult head elav FACS 2

characteristic: Submitter Id

text: 2 day

text: ArrayExpress

text: fruit fly

text: adult

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head (neurons)

text: Drosophila melanogaster

text: elav-GAL4, UAS-H2B-GFP

text: adult head elav FACS 2


E-MTAB-1117:embryo 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: E-MTAB-1117:embryo 3

text: 0 day

text: ArrayExpress

text: fruit fly

text: embryo

text: Drosophila melanogaster

text: Drosophila melanogaster

text: embryo

text: Drosophila melanogaster

text: wild-type (2202U2)

text: embryo 3


E-MTAB-1117:adult head repo FACS 1

text: EMBL-EBI

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-1117:adult head repo FACS 1

text: 2 day

text: ArrayExpress

text: fruit fly

characteristic: common name

text: adult

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: head (glia)

text: Drosophila melanogaster

text: adult head repo FACS 1


E-MTAB-1117:adult head elav FACS 1

text: EMBL-EBI

text: public

text: E-MTAB-1117:adult head elav FACS 1

text: 2 day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: description

text: adult

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head (neurons)

text: Drosophila melanogaster

text: elav-GAL4, UAS-H2B-GFP

text: adult head elav FACS 1


text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 GFP tagged

characteristic: ArrayExpress-Genotype

text: Chromotek GFP trap Agarose

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: Bl6 MeCP2 GFP

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: house mouse

text: Mus musculus

text: crosslinked and sonicated chromatin= Input from GFP high sample (MeCP2 GFP from 15 BL 6 male)

characteristic: sample description

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: strain


text: Glia

text: Adult

text: MeCP2 GFP tagged

text: Chromotek GFP trap Agarose

text: Brain

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: crosslinked and sonicated chromatin= Input from GFP low sample (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: Neurons

characteristic: ArrayExpress-CellType

text: Adult

characteristic: ArrayExpress-DevelopmentalStage

text: MeCP2 GFP tagged

characteristic: ArrayExpress-Genotype

text: Chromotek GFP trap Agarose

characteristic: ArrayExpress-Immunoprecipitate

text: Brain

characteristic: ArrayExpress-OrganismPart

text: wildtype

text: male

text: Mus musculus

text: Bl6 MeCP2 GFP

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: ChIP DNA from GFP high neurons (MeCP2 GFP from 15 BL 6 male)

text: Mus musculus

text: Bl6 MeCP2 GFP


text: Glia

text: Adult

text: MeCP2 GFP tagged

text: Chromotek GFP trap Agarose

text: Brain

text: wildtype

characteristic: ArrayExpress-Phenotype

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: Bl6 MeCP2 GFP

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

text: house mouse

text: Mus musculus

text: ChIP DNA from GFP low glia (MeCP2 GFP from 15 BL 6 male)

characteristic: sample description

text: Mus musculus

text: Bl6 MeCP2 GFP

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CEA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: All strains were grown in YPD rich medium at 30 degrees C to 0.6 OD600, then shifted at 37 degrees C for 45min except for Rad2-HA strain. The cultures were cross-linked with 1% formaldehyde for 10 min The cell pellets are resuspended in 1 ml of FA/SDS/PMSF buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF) and transferred to 1.5 mL Eppendorf tubes. 0.75 mL glass beads (425-600 um, Sigma) are then added to each tube, and the yeast cells were lysed at 4 degrees C for 15 min by vortexing. Pierce the bottom of a 12 ml Greiner tube with a hot needle (0.7x30 mm or 0.5x15 mm) and place in a Falcon50 with a pierced cap. The lysates are transfered in the pierced tubes. Wash each Epp with twice 1 ml of FA/SDS/PMSF buffer (4 ml per extract). Spin down 1 min/2000 rpm. Gently resuspend the pellet with a 5 ml pipet and transfer in a sterile 15 ml Corex tube. Spin down 20 min / 12000 rpm / 4 degrees C in a JA17 rotor (Beckmann). Vacuum the supernatant. The cross-linked chromatin appears as a transparent layer around the pellet. Transfer the pellet in a 2 ml Epp with a burned Pasteur pipet. Add 0.8 ml of FA/SDS/PMSF buffer in the Corex and resuspend with the Pasteur pipet. Transfer in the Epp. Wash the Corex with 0.8 ml FA/SDS/PMSF buffer. Homogenize the Epp with the Pasteur pipet and ihncubate on a rotating wheal at 4 degrees C for 1 to 2 hours.Spin down 20 min / 12000 rpm / 4 degrees C. Vacuum the supernatant and resuspend in 1.6 ml FA/SDS/PMSF buffer with a burned Pasteur pipet.Place the tubes in a mix water / ice / NaCl. Sonicator settings: Pulse - 60% - Power 4. Sonicate 3 times 40 sec with 20 sec intervals. Split in 200ul aliquots and performe an additional sonication step with Bioruptor (Diagenode), 6 cycles of 30s with medium intensity setting. Average size of fragments obtained is 200bp.Transfer in a 12 ml Greiner tube and add 0.5 ml FA/SDS/PMSF buffer. Incubate on a rotating wheal for 30 min to 1 hour at 4 degrees C. Spin down 30 min / 10000 rpm / 4 degrees C on a JA20 rotor (Beckmann).Transfer the supernatant in a new tube. Aliquot the supernatant (the chromatin is now soluble). Aliquots of 650 ul and freeze in liquid nitrogen.Chromatin fragment control: Take an aliquot of 100 ul of chromatin and add 25 ul Pronase buffer 5X and 6.25 ul of Pronase (20 mg/ml in H20). Incubate 1 hour at 37 degrees C and O/N at 65 degrees C (digestproteins and reverse crosslink). Add 3.5 ul of RNaseA 1mg/ml and incubate 1 hour at 37 degrees C. Purify on a Quiagen PCR purification column (Elute wih 50 ul Tris 10 mM pH8.5). Load on gel (1.5% in TAE-100V).Immunoprecipitation:Wash 50 ul of Dynabeads Ig 4 times with 500 ul PBS BSA 0.1% buffer. Resuspend in 100 ul PBS BSA 0.1 % buffer. Add antibody (5ul 8WG16 or 1ul 12CA5). Incubate 30 min at 1300 rpm in an Eppendorf shaker at 30 degrees C. Wash 2X 500 ul PBS BSA 0.1%. Wash 1X 500 ul PBS BSA 0.1% with shaking 10 min at 1300 rpm / 30 degrees C. Wash 1X 500 ul PBS BSA 0.1%.Spin down the chromatin from the -80 degrees C for 15 min / 12000 rpm / 4 degrees C. Add 50 ul of PBS BSA 10mg/ml to the beads and then 500 ul of chromatin.Incubate 2h at 21 degrees C in an Eppendorf shaker (1300 rpm). Resuspend with 500 ul FA/SDS and change tube. Wash 2X 1 ml FA/SDS + NaCl (final concentration 500 mM). Wash 1X 1 ml FA/SDS + NaCl with shaking 10 min at 1300 rpm / 21 degrees C. Wash 1X with 500 ul IP buffer (Tris pH7.5 125 mM, EDTA 25 mM, SDS 2.5%). Wash 1X with 500 ul TE. Elute with 125 ul Pronase Buffer 1X for 20 min at 65 degrees C in an Eppendorf shaker at 600 rpm. Cool down at RT. Add 6.25 ul Pronase to the supernatant. Incubate 1h at 37 degrees C and O/N at 65 degrees C. Add 25 ul of Pronase buffer 5X and 6.25 ul of Pronase to 100 ul of chromatin remaining from the initial tube (total DNA).Incubate 1h at 37 degrees C and O/N at 65 degrees C. Add 3.5 ul RNAseA (1mg/ml) and incubate 1h at 37 degrees C. Purify DNA on a Qiagen PCR purification column.

characteristic: description

text: tagging

characteristic: genetic modification

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 MED17::3HA::HIS3 med10::KanMX6 // MED10 CEN TRP1

characteristic: genotype

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: mating type a

characteristic: sex

characteristic: title


text: EMBL-EBI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 2 week

characteristic: age

text: ArrayExpress

text: long-grained rice

characteristic: common name

characteristic: cultivar

text: Protocols: Oryza sativa L. Indica IR29 seedlings were grown in growth chambers to the 2-3 leaf stage. At 2 weeks old, they were subjected to salt stress treatment by watering with 300 mM NaCl solution (or normal watering for control plants). Treatment was applied at 10:00 PST (2 hours post-dawn). Seedling shoots (stem and leaves) were sampled at 1 hour, 2 hours, 5 hours, 10 hours, and 24 hours post-treatment. Total cellular RNA was extracted using RNA Plant Reagent (Life Technologies) and treated with RNase-free Turbo DNase (Life Technologies) followed by a clean-up step using Plant RNeasy kits (Qiagen). Concentration, integrity and extent of contamination by ribosomal RNA were assessed using ND-1000 spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 (Agilent Technologies). For details see Fox et al. (2009) Applications for Ultra-high-Throughput Sequencing. In: Belostotsky (ed.), Plant Systems Biology, vol. 553, pp. 79--108. Libraries were prepared using the TruSeq(TM) RNA Sample Preparation Kits (v2).

text: seedling, two leaves visible, three leaves visible

characteristic: developmental stage

text: Oryza sativa Indica group

characteristic: organism

text: Oryza sativa Indica Group

text: shoot, vascular leaf

characteristic: organism part

text: Oryza sativa Indica Group

text: fresh specimen

characteristic: specimen with known storage state

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Arizona

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University of Arizona

text: 20 month

text: ArrayExpress

text: chicken

text: Protocols: The source of the RNA was a variety of red jungle fowl tissues. Total RNA was extracted according to the Qiagen miRNAeasy protocol. MRNAseq libraries were constructed according to the Illumina TruSeq RNA protocol.

text: adult

text: inbred line

characteristic: environmental history

text: Gallus gallus

text: Gallus gallus

text: lung

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text: Gallus gallus

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text: Gallus gallus

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text: Gallus gallus

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text: Gallus gallus

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text: chicken

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text: Gallus gallus

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text: Gallus gallus

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text: Gallus gallus

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text: University of Arizona

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text: University of Arizona

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text: Gallus gallus

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text: Gallus gallus

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E-MTAB-1919:Human islets (HI 32)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1919:Human islets (HI 32)

tag: Namespace:Institut d'Investigacions Biomediques August Pi i Sunyer

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: Human islets were fixed and sonicated as described previously (Nammo et al, 2012). Briefly, frozen crosslinked cell pellets of 4,000 islets were thawed on ice in 1 mL lysis buffer and disrupted with five 1-min cycles with 0.5 mm glass beads (BioSpec). Samples were sonicated for 10-20 cycles of 30 pulses each (1s on and 0.5 s off) using a Brandson Sonifier 450D at 15 % amplitude. The size for the bulk of chromatin obtained with this procedure was checked to be in the range from 200-1000 bp. For ChIPs, 300-400 human islet equivalents (IEQs) were pre-cleared with A/G sepharose beads (Ge Healthcare) for 1 h, rotating at 4 degrees C, and then incubated with each antibody. Afterwards, samples were rotated for 2 hr at 4 degrees C with protein A/G sepharose beads and then sequentially washed with low salt, high salt, LiCl and TE buffers. Next, chromatin was eluted with SDS 1% buffer and sequentially treated with RNAse (greater than 5 hr at 65 degrees C) and Proteinase K (overnight at 45 degrees C). Finally, DNA was extracted with phenol-chloroform followed by ethanol precipitation. The immunoprecipitated DNA was modified for sequencing following the Illumina sequencing following the manufacturer protocol (Preparing Sample for ChIP Sequencing of DNA (11257047 Rev A)).

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 32)


E-MTAB-1919:Human islets (HI 22)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut d'Investigacions Biomediques August Pi i Sunyer

text: public

text: E-MTAB-1919:Human islets (HI 22)

text: ArrayExpress

characteristic: broker name

text: human

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text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 22)


E-MTAB-1919:Human islets (HI 25)

text: public

text: E-MTAB-1919:Human islets (HI 25)

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 25)


E-MTAB-1919:Human islets (HI 45)

characteristic: INSDC center name

text: public

text: E-MTAB-1919:Human islets (HI 45)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 45)

characteristic: title


E-MTAB-1919:Human islets (HI 21)

characteristic: INSDC first public

text: public

text: E-MTAB-1919:Human islets (HI 21)

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 21)


E-MTAB-1919:Human islets (HI 101)

text: public

text: E-MTAB-1919:Human islets (HI 101)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: islet of Langerhans

characteristic: organism part

text: Homo sapiens

text: Human islets (HI 101)


E-MTAB-1919:Human islets (HI 87)

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-1919:Human islets (HI 87)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 87)


E-MTAB-1919:Human islets (HI 34)

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-1919:Human islets (HI 34)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 34)


E-MTAB-1919:Human islets (HI 118)

characteristic: INSDC last update

text: public

text: E-MTAB-1919:Human islets (HI 118)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 118)


E-MTAB-1919:Human islets (HI 81)

text: public

characteristic: INSDC status

text: E-MTAB-1919:Human islets (HI 81)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 81)


E-MTAB-1919:Human islets (HI 88)

text: public

characteristic: SRA accession

text: E-MTAB-1919:Human islets (HI 88)

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 88)


E-MTAB-1919:Human islets (HI 102)

text: public

text: E-MTAB-1919:Human islets (HI 102)

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Homo sapiens

text: Homo sapiens

text: islet of Langerhans

text: Homo sapiens

text: Human islets (HI 102)


text: iPS

characteristic: ArrayExpress-CellType

text: Homo sapiens

characteristic: ArrayExpress-Species

characteristic: ENA-LAST-UPDATE

text: SC

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text: human

text: Homo sapiens

text: PCR products obtained from each target loci using genomic DNA from Cas9-treated iPS stem cells

characteristic: sample description

text: Homo sapiens


text: Plasmodium falciparum

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text: SC

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text: Plasmodium falciparum

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text: ZfRef2c1-sc-2013-10-09T11:15:50Z-1694915

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text: Zebrafish

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text: ZfRef2d1-sc-2013-10-09T11:15:51Z-1694916

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text: Zebrafish

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characteristic: INSDC last update

text: public

text: ZfRef2e1-sc-2013-10-09T11:15:52Z-1694917

text: zebrafish

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text: Danio rerio

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text: Zebrafish

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text: SC

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characteristic: INSDC status

text: ZfRef2f1-sc-2013-10-09T11:15:54Z-1694918

text: zebrafish

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text: Zebrafish

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text: ZfRef2g1-sc-2013-10-09T11:15:55Z-1694919

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text: Zebrafish

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text: ZfRef2h1-sc-2013-10-09T11:15:57Z-1694920

characteristic: Submitter Id

text: zebrafish

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text: Zebrafish

text: AB/IND

text: SC

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text: ZfRef2a2-sc-2013-10-09T11:15:59Z-1694921

text: zebrafish

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text: Zebrafish

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text: ZfRef2b2-sc-2013-10-09T11:16:00Z-1694922

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2c2-sc-2013-10-09T11:16:01Z-1694923

text: zebrafish

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text: Zebrafish

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text: SC

text: public

text: ZfRef2d2-sc-2013-10-09T11:16:02Z-1694924

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2e2-sc-2013-10-09T11:16:04Z-1694925

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2f2-sc-2013-10-09T11:16:05Z-1694926

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2g2-sc-2013-10-09T11:16:06Z-1694927

text: zebrafish

text: Danio rerio

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text: Zebrafish

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text: SC

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text: ZfRef2h2-sc-2013-10-09T11:16:07Z-1694928

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2a3-sc-2013-10-09T11:16:08Z-1694929

text: zebrafish

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text: Zebrafish

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text: ZfRef2b3-sc-2013-10-09T11:16:09Z-1694930

text: zebrafish

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text: Zebrafish

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text: ZfRef2c3-sc-2013-10-09T11:16:11Z-1694931

text: zebrafish

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text: Zebrafish

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text: ZfRef2d3-sc-2013-10-09T11:16:12Z-1694932

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2e3-sc-2013-10-09T11:16:13Z-1694933

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text: ZfRef2f3-sc-2013-10-09T11:16:15Z-1694934

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text: Zebrafish

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text: ZfRef2g3-sc-2013-10-09T11:16:16Z-1694935

text: zebrafish

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text: Zebrafish

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text: ZfRef2h3-sc-2013-10-09T11:16:17Z-1694936

text: zebrafish

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text: Zebrafish

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text: ZfRef2a4-sc-2013-10-09T11:16:18Z-1694937

text: zebrafish

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text: Zebrafish

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text: ZfRef2b4-sc-2013-10-09T11:16:19Z-1694938

text: zebrafish

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text: Zebrafish

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text: SC

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text: ZfRef2c4-sc-2013-10-09T11:16:20Z-1694939

text: zebrafish

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text: Zebrafish

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text: ZfRef2d4-sc-2013-10-09T11:16:22Z-1694940

text: zebrafish

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text: Zebrafish

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text: SC

text: public

text: ZfRef2e4-sc-2013-10-09T11:16:23Z-1694941

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

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text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef2f4-sc-2013-10-09T11:16:24Z-1694942

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

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text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef2g4-sc-2013-10-09T11:16:26Z-1694943

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text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e4-sc-2013-10-09T11:18:23Z-1695037

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f4-sc-2013-10-09T11:18:24Z-1695038

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g4-sc-2013-10-09T11:18:26Z-1695039

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h4-sc-2013-10-09T11:18:27Z-1695040

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a5-sc-2013-10-09T11:18:28Z-1695041

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b5-sc-2013-10-09T11:18:29Z-1695042

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c5-sc-2013-10-09T11:18:30Z-1695043

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d5-sc-2013-10-09T11:18:31Z-1695044

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e5-sc-2013-10-09T11:18:33Z-1695045

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f5-sc-2013-10-09T11:18:34Z-1695046

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g5-sc-2013-10-09T11:18:35Z-1695047

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h5-sc-2013-10-09T11:18:36Z-1695048

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a6-sc-2013-10-09T11:18:37Z-1695049

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b6-sc-2013-10-09T11:18:38Z-1695050

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c6-sc-2013-10-09T11:18:39Z-1695051

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d6-sc-2013-10-09T11:18:40Z-1695052

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e6-sc-2013-10-09T11:18:41Z-1695053

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f6-sc-2013-10-09T11:18:43Z-1695054

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g6-sc-2013-10-09T11:18:44Z-1695055

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h6-sc-2013-10-09T11:18:46Z-1695056

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a7-sc-2013-10-09T11:18:47Z-1695057

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b7-sc-2013-10-09T11:18:48Z-1695058

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c7-sc-2013-10-09T11:18:50Z-1695059

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d7-sc-2013-10-09T11:18:52Z-1695060

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e7-sc-2013-10-09T11:18:53Z-1695061

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f7-sc-2013-10-09T11:18:54Z-1695062

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g7-sc-2013-10-09T11:18:55Z-1695063

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h7-sc-2013-10-09T11:18:56Z-1695064

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a8-sc-2013-10-09T11:18:58Z-1695065

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b8-sc-2013-10-09T11:18:59Z-1695066

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c8-sc-2013-10-09T11:19:00Z-1695067

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d8-sc-2013-10-09T11:19:01Z-1695068

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e8-sc-2013-10-09T11:19:02Z-1695069

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f8-sc-2013-10-09T11:19:03Z-1695070

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g8-sc-2013-10-09T11:19:05Z-1695071

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h8-sc-2013-10-09T11:19:06Z-1695072

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a9-sc-2013-10-09T11:19:07Z-1695073

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b9-sc-2013-10-09T11:19:08Z-1695074

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c9-sc-2013-10-09T11:19:09Z-1695075

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d9-sc-2013-10-09T11:19:11Z-1695076

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e9-sc-2013-10-09T11:19:12Z-1695077

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f9-sc-2013-10-09T11:19:13Z-1695078

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g9-sc-2013-10-09T11:19:14Z-1695079

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h9-sc-2013-10-09T11:19:16Z-1695080

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a10-sc-2013-10-09T11:19:17Z-1695081

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b10-sc-2013-10-09T11:19:18Z-1695082

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c10-sc-2013-10-09T11:19:19Z-1695083

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d10-sc-2013-10-09T11:19:21Z-1695084

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e10-sc-2013-10-09T11:19:22Z-1695085

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f10-sc-2013-10-09T11:19:23Z-1695086

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g10-sc-2013-10-09T11:19:24Z-1695087

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h10-sc-2013-10-09T11:19:25Z-1695088

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a11-sc-2013-10-09T11:19:26Z-1695089

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b11-sc-2013-10-09T11:19:27Z-1695090

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c11-sc-2013-10-09T11:19:28Z-1695091

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d11-sc-2013-10-09T11:19:29Z-1695092

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e11-sc-2013-10-09T11:19:30Z-1695093

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f11-sc-2013-10-09T11:19:31Z-1695094

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g11-sc-2013-10-09T11:19:32Z-1695095

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h11-sc-2013-10-09T11:19:33Z-1695096

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3a12-sc-2013-10-09T11:19:35Z-1695097

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3b12-sc-2013-10-09T11:19:36Z-1695098

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3c12-sc-2013-10-09T11:19:37Z-1695099

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3d12-sc-2013-10-09T11:19:38Z-1695100

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3e12-sc-2013-10-09T11:19:39Z-1695101

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3f12-sc-2013-10-09T11:19:40Z-1695102

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3g12-sc-2013-10-09T11:19:42Z-1695103

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


text: Zebrafish

text: AB/IND

text: SC

text: public

text: ZfRef3h12-sc-2013-10-09T11:19:43Z-1695104

text: zebrafish

text: Danio rerio

text: MGH map

text: Danio rerio

text: AB/IND


E-MTAB-1930:pig 17_aortic arch

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 17_aortic arch

tag: Namespace:University of Pennsylvania

text: 6 month

characteristic: age

text: about 250 pound

characteristic: body weight

text: ArrayExpress

characteristic: broker name

text: pig

text: Protocols: Endothelial cells were obtained from adult pigs immediately after euthanasia at a local slaughterhouse. Ascending and descending aortas were harvested, and the vessel lumen was rinsed with ice-cold PBS. Endothelial cells were freshly harvested by gentle scraping of regions (1-2 cm2) located at the inner curvature of the aortic arch (AA) and from nearby descending thoracic aorta (DT) representing athero-susceptible and athero-protective sites respectively. Genomic DNA was isolated by DNeasy Kit (Qiagen). The quality of DNA was evaluated by NanoDrop 1000 Spectrophotometer and TapeStation 2200 (Agilent Technologies). Only high quality DNA (260/280>1.8) was used in the study. The sex of each pig was determined by the presence (male) or absence (female) of sex determining region Y (SRY) gene in the Y chromosome. Genomic DNA randomly selected from two regions of 6 female and 6 male pigs were were used as biological replicates in the methylated DNA immunoprecipitation sequencing (MeDIP-seq) study. One microgram genomic DNA was sheared into ~200bp fragments using a Covaris S2 sonication system (Covaris, Woburn, MA) according to the manufacturer’s settings. MeDIP DNA Libraries were constructed using DNA Library Prep kit for Illumina (NEB) consisting of DNA end repair, 3'-dA tailing and adapter ligation. After adapter ligation, the DNA was immunoprecipitated by MagMeDIP (Diagenode) with antibody against methylated DNA according to the manufacturer's protocol. The MeDIP DNA was amplified by PCR using index primers and phusion high-fidelity DNA polymerase (NEB). The PCR cycles consisted of 98 C 30s, 15 cycles of 98 C 10s, 65 C 30s, 72 C 30s, followed by prolonged extension for 5 min at 72 C. After amplification and purification, MeDIP DNA libraries with 50-100bp insert size were selected by Sage Science’s Pippin Prep in 2% agarose gel. The libraries Library quality and quantity were evaluated using an Agilent 2100 Analyzer and DNA 1000 chips. The specificity and efficiency of MeDIP enrichment were verified by qPCR with primers targeting ACTA2 promoter (methylated in endothelial cells) and UBB promoter (unmethylated in endothelial cells). The mean ratio of methylated/unmethylated DNA was 244,700 fold, indicating highly enrichment of methylated DNA.

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

text: Sus scrofa

text: aortic arch

characteristic: organism part

text: Sus scrofa

text: female

characteristic: sex

text: Landrace x Yorkshire

text: pig 17_aortic arch

characteristic: title


E-MTAB-1930:pig 12_descending thoracic

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 12_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: male

text: Landrace x Yorkshire

characteristic: strain

text: pig 12_descending thoracic


E-MTAB-1930:pig 5_descending thoracic

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 5_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 5_descending thoracic


E-MTAB-1930:pig 12_aortic arch

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 12_aortic arch

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

characteristic: body weight

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 12_aortic arch


E-MTAB-1930:pig 14_descending thoracic

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 14_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

characteristic: organism part

text: Sus scrofa

text: male

characteristic: sex

text: Landrace x Yorkshire

text: pig 14_descending thoracic


E-MTAB-1930:pig 10_aortic arch

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 10_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 10_aortic arch

characteristic: title


E-MTAB-1930:pig 15_descending thoracic

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 15_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: female

text: Landrace x Yorkshire

characteristic: strain

text: pig 15_descending thoracic


E-MTAB-1930:pig 10_descending thoracic

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 10_descending thoracic

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 10_descending thoracic


E-MTAB-1930:pig 7_descending thoracic

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 7_descending thoracic

text: 6 month

text: about 250 pound

characteristic: body weight

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

characteristic: organism part

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 7_descending thoracic


E-MTAB-1930:pig 15_aortic arch

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 15_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: female

characteristic: sex

text: Landrace x Yorkshire

text: pig 15_aortic arch


E-MTAB-1930:pig 5_aortic arch

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 5_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 5_aortic arch

characteristic: title


E-MTAB-1930:pig 13_descending thoracic

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 13_descending thoracic

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: male

text: Landrace x Yorkshire

characteristic: strain

text: pig 13_descending thoracic


E-MTAB-1930:pig 14_aortic arch

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 14_aortic arch

text: 6 month

text: about 250 pound

characteristic: body weight

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 14_aortic arch


E-MTAB-1930:pig 4_descending thoracic

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 4_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

characteristic: organism part

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 4_descending thoracic


E-MTAB-1930:pig 4_aortic arch

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 4_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

characteristic: sex

text: Landrace x Yorkshire

text: pig 4_aortic arch


E-MTAB-1930:pig 2_aortic arch

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 2_aortic arch

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 2_aortic arch

characteristic: title


E-MTAB-1930:pig 3_aortic arch

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 3_aortic arch

text: 6 month

text: about 250 pound

characteristic: body weight

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: female

text: Landrace x Yorkshire

characteristic: strain

text: pig 3_aortic arch


E-MTAB-1930:pig 2_descending thoracic

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 2_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 2_descending thoracic


E-MTAB-1930:pig 11_descending thoracic

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 11_descending thoracic

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

characteristic: organism part

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 11_descending thoracic


E-MTAB-1930:pig 13_aortic arch

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 13_aortic arch

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

characteristic: sex

text: Landrace x Yorkshire

text: pig 13_aortic arch


E-MTAB-1930:pig 17_descending thoracic

text: University of Pennsylvania

text: public

text: E-MTAB-1930:pig 17_descending thoracic

text: 6 month

text: about 250 pound

characteristic: body weight

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: birth

characteristic: initial time point

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 17_descending thoracic

characteristic: title


E-MTAB-1930:pig 7_aortic arch

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Pennsylvania

characteristic: INSDC center name

text: public

text: E-MTAB-1930:pig 7_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

characteristic: common name

text: birth

text: Sus scrofa

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: female

text: Landrace x Yorkshire

characteristic: strain

text: pig 7_aortic arch


E-MTAB-1930:pig 11_aortic arch

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Pennsylvania

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-1930:pig 11_aortic arch

text: 6 month

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: aortic arch

text: Sus scrofa

text: male

text: Landrace x Yorkshire

text: pig 11_aortic arch


E-MTAB-1930:pig 3_descending thoracic

text: University of Pennsylvania

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-1930:pig 3_descending thoracic

characteristic: Submitter Id

text: 6 month

characteristic: age

text: about 250 pound

text: ArrayExpress

text: pig

text: birth

text: Sus scrofa

text: Sus scrofa

text: descending thoracic aorta

characteristic: organism part

text: Sus scrofa

text: female

text: Landrace x Yorkshire

text: pig 3_descending thoracic


text: ESC

characteristic: ArrayExpress-CellType

text: embryonic stem cell line

characteristic: ArrayExpress-DevelopmentalStage

characteristic: ArrayExpress-Genotype

text: 2i media

characteristic: ArrayExpress-GrowthCondition

text: male

characteristic: ArrayExpress-Sex

text: Mus Musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: mES_2i_sample2-sc-2013-10-10T09:41:15Z-1720332

text: house mouse

text: Mus musculus

text: mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL/6Ncr male x 129S6/SvEvTac female

characteristic: sample description

text: Mus musculus


text: ESC

text: embryonic stem cell line

text: G4 ES cell line from a male blastocyst from a C57BL/6Ncr male x 129S6/SvEvTac female

text: serum/LIF

text: male

text: Mus Musculus

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: mES_lif_sample2-sc-2013-10-10T09:41:33Z-1720333

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: ESC

text: embryonic stem cell line

text: serum/LIF

text: male

text: Mus Musculus

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: mES_alt2i_sample2a-sc-2013-10-10T09:41:49Z-1720334

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus


text: ESC

characteristic: ArrayExpress-CellType

text: embryonic stem cell line

text: serum/LIF

text: male

text: Mus Musculus

text: SC

text: public

characteristic: SRA accession

text: mES_alt2i_sample2b-sc-2013-10-10T09:42:07Z-1720335

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

tag: Namespace:University of Milan. Dept. of Biosciences

text: 0 day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: Plants of Arabidopsis thaliana ecotype Col-0 were grown under long day conditions (LD; 16-hours light/8-h dark). Tissue was sectioned into slices with a Leica RM 2155 microtome, distended on pure methanol (Schmid et al., 2012) on Leica PET membrane frameslides, dried at 45 degrees C for 45 minutes, washed for 15 minutes in pure xylol to remove Paraplast, dried again at room temperature for 10 minutes and immediately dissected with a Leica LMD6000 laser microdissector. Material from 3 slides was pooled in a single tube and immediately after microdissection 10 ul of XB buffer from Arcturus Picopure RNA extraction Kit (Life Technologies) was added. Samples were then heated at 42 degrees C for 30 minutes, centrifuged at 800 r.c.f for 2 minutes and stored at -80. Before proceeding with the extraction, material from 5 different tubes were pooled in one; therefore one single replicate contains material microdissected from 15 inflorescences, each harvested from a different plant. RNA extraction was then performed using the Arcturus Picopure RNA extraction kit as specified by the manufacturers instructions. RNA integrity and concentration was tested with an Agilent 2100 Bioanalyzer. Retro-transcription and amplification were performed using the Ovation RNAseq V2 kit (NuGen technologies) as specified by the manufacturers instructions.

characteristic: description

text: undifferentiated meristem

characteristic: developmental stage

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

characteristic: organism part

text: Arabidopsis thaliana

text: frozen specimen

characteristic: specimen with known storage state

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Milan. Dept. of Biosciences

characteristic: INSDC center name

text: public

text: 3 day

text: ArrayExpress

text: thale cress

text: meristem stage 3

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

text: Arabidopsis thaliana

text: frozen specimen


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 1 day

text: ArrayExpress

text: thale cress

characteristic: common name

text: meristem stage 1

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

text: Arabidopsis thaliana

text: frozen specimen


text: public

characteristic: INSDC status

characteristic: SRA accession

text: 0 day

text: ArrayExpress

text: thale cress

text: undifferentiated meristem

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: flower

text: Arabidopsis thaliana

text: frozen specimen


text: Mesoderm

characteristic: ArrayExpress-CellType

text: mixed

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: C57Bl/6 x Sv129

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv129

characteristic: sample description

text: Mus musculus

text: C57Bl/6 x Sv129

characteristic: strain


text: Mesoderm

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv130

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv131

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC first public

text: public

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv132

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

text: SC

characteristic: INSDC last update

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv133

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv134

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

characteristic: ArrayExpress-CellType

text: mixed

text: Mus musculus

text: C57Bl/6 x Sv129

text: SC

text: public

text: house mouse

text: Mus musculus

characteristic: organism

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv135

text: Mus musculus

text: C57Bl/6 x Sv129


text: Mesoderm

text: mixed

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: C57Bl/6 x Sv129

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNA extracted from mesoderm cells of mouse strain C57Bl/6 x Sv137

text: Mus musculus

text: C57Bl/6 x Sv129