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text: Mus musculus

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text: inguinal white adipose tissue

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text: Preadipocyte-B


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text: Protocols: 1mg/kg for 7 consecutive days Total RNA was extracted from FACS-isolated GFP+ adipocytes or preadipocytes using the RNeasy Micro Kit (Qiagen). RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with RIN of >7.0 were subjected to RNA-seq. Libraries were constructed from minimum of 22 ng of total RNA as previously described (Nature 504 (7478), 163-167)

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type: has member


E-MTAB-3978:White Adipocyte-Ctrl

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tag: Namespace:Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery University of Salerno, SA, Baronissi, Italy

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text: Protocols: Resected specimens from 17 patients, each with multiple hepatocellular nodules (HN) well representative of different steps of human hepatocarcinogenesis were collected and included in this study Total RNA was extracted from FFPE sections of human livers using RNeasy FFPE Kit (QIAGEN GmbH, Hilden, Germany) in duplicates and quantitated with NanoDrop-1000 spectrophotometer 1Izg of total RNA/samples was used for library preparation with Illumina TruSeq small RNA sample preparation Kit

text: hepatocellular carcinoma

characteristic: disease

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text: Homo sapiens

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