text: Staphylococcus aureus

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text: Staphylococcus aureus subsp. aureus TW20

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text: Staphylococcus aureus

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text: Staphylococcus aureus subsp. aureus TW20

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Dongdo

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text: Bacterial communities of Dokdo

characteristic: title

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Seodo

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text: metagenome

text: Bacterial communities of Dokdo


text: Haemophilus parasuis

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text: IN_13000_1_J2013-sc-2013-08-29T15:26:20Z-1686346

text: [Haemophilus] parasuis

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text: IN_13000_2_J2013-sc-2013-08-29T15:26:23Z-1686347

text: [Haemophilus] parasuis

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text: [Haemophilus] parasuis

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text: [Haemophilus] parasuis

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text: IN_13000_5_J2013-sc-2013-08-29T15:26:26Z-1686350

text: [Haemophilus] parasuis

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text: Haemophilus parasuis

text: SC

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text: Medium_Out_13000_1_J2013-sc-2013-08-29T15:26:28Z-1686351

text: [Haemophilus] parasuis

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text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_2_J2013-sc-2013-08-29T15:26:29Z-1686352

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_3_J2013-sc-2013-08-29T15:26:30Z-1686353

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_4_J2013-sc-2013-08-29T15:26:31Z-1686354

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_5_J2013-sc-2013-08-29T15:26:33Z-1686355

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


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text: SC

text: public

text: IVOC_N_Out_13000_1_J2013-sc-2013-08-29T15:26:34Z-1686356

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_2_J2013-sc-2013-08-29T15:26:35Z-1686357

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_3_J2013-sc-2013-08-29T15:26:37Z-1686358

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_4_J2013-sc-2013-08-29T15:26:38Z-1686359

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_5_J2013-sc-2013-08-29T15:26:39Z-1686360

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_1_J2013-sc-2013-08-29T15:26:40Z-1686361

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

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text: IVOC_T_Out_13000_2_J2013-sc-2013-08-29T15:26:41Z-1686362

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


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text: IVOC_T_Out_13000_3_J2013-sc-2013-08-29T15:26:42Z-1686363

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


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text: IVOC_T_Out_13000_4_J2013-sc-2013-08-29T15:26:44Z-1686364

text: [Haemophilus] parasuis

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text: IVOC_T_Out_13000_5_J2013-sc-2013-08-29T15:26:45Z-1686365

text: [Haemophilus] parasuis

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text: IN_13000_A_A2013-sc-2013-08-29T15:26:46Z-1686366

text: [Haemophilus] parasuis

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text: IN_13000_B_A2013-sc-2013-08-29T15:26:47Z-1686367

text: [Haemophilus] parasuis

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text: IN_13000_C_A2013-sc-2013-08-29T15:26:48Z-1686368

text: [Haemophilus] parasuis

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text: IN_13000_D_A2013-sc-2013-08-29T15:26:49Z-1686369

text: [Haemophilus] parasuis

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text: Haemophilus parasuis

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text: IN_13000_E_A2013-sc-2013-08-29T15:26:50Z-1686370

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

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text: public

text: Medium_Out_13000_A_A2013-sc-2013-08-29T15:26:51Z-1686371

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_B_A2013-sc-2013-08-29T15:26:53Z-1686372

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_C_A2013-sc-2013-08-29T15:26:54Z-1686373

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_D_A2013-sc-2013-08-29T15:26:55Z-1686374

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: Medium_Out_13000_E_A2013-sc-2013-08-29T15:26:56Z-1686375

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_A_A2013-sc-2013-08-29T15:26:57Z-1686376

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_B_A2013-sc-2013-08-29T15:26:58Z-1686377

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_C_A2013-sc-2013-08-29T15:27:00Z-1686378

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_D_A2013-sc-2013-08-29T15:27:01Z-1686379

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_N_Out_13000_E_A2013-sc-2013-08-29T15:27:02Z-1686380

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_A_A2013-sc-2013-08-29T15:27:03Z-1686381

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_B_A2013-sc-2013-08-29T15:27:04Z-1686382

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_C_A2013-sc-2013-08-29T15:27:05Z-1686383

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_D_A2013-sc-2013-08-29T15:27:07Z-1686384

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: Haemophilus parasuis

text: SC

text: public

text: IVOC_T_Out_13000_E_A2013-sc-2013-08-29T15:27:08Z-1686385

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis


text: JHI

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text: Ribes nigrum

text: Ribes nigrum

text: Genotyping-by-sequencing in Ribes nigrum S10xS36 population


text: Salmonella enterica subsp. enterica serovar Typhi

text: SC

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text: BRD948_WM_A00-sc-2013-09-02T11:56:45Z-1686815


text: SC

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text: BRD948_WM_B00-sc-2013-09-02T11:56:53Z-1686816


text: SC

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text: SC

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text: BRD948_WM_C06-sc-2013-09-02T11:57:02Z-1686823


text: SC

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text: BRD948_WM_A24-sc-2013-09-02T11:57:03Z-1686824


text: SC

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text: BRD948_WM_B24-sc-2013-09-02T11:57:04Z-1686825


text: SC

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text: BRD948_WM_C24-sc-2013-09-02T11:57:06Z-1686826


text: Mycobacterium tuberculosis

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text: Mycobacterium tuberculosis

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text: Mycobacterium tuberculosis

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text: TB062_09-sc-2013-09-03T08:16:39Z-1686392

text: Mycobacterium tuberculosis

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text: TB013_09-sc-2013-09-03T08:17:04Z-1686413

text: Mycobacterium tuberculosis

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text: public

text: TB062_09-sc-2013-09-03T08:17:07Z-1686415

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB163_08-sc-2013-09-03T08:17:11Z-1686419

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB191_10-sc-2013-09-03T08:17:12Z-1686420

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB674_11-sc-2013-09-03T08:17:13Z-1686421

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB683_11-sc-2013-09-03T08:17:14Z-1686422

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB721_11-sc-2013-09-03T08:17:15Z-1686423

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Mycobacterium tuberculosis

text: SC

text: public

text: TB1414_12-sc-2013-09-03T08:17:17Z-1686424

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis


text: Bordetella pertussis

text: SC

text: public

text: Bp_18323-sc-2013-09-03T11:31:22Z-1687003

text: Bordetella pertussis

text: Bordetella pertussis


text: Bordetella pertussis

text: SC

text: public

text: Bordetella pertussis

text: Bordetella pertussis


text: GigaScience

text: public

text: ArrayExpress

characteristic: broker name

text: Protocols: Manual collection of specimen from natural habitat (cave), crushed and preserved in liquid RNA-later (Qiagen, USA). Total RNA was extract using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions. Messenger RNA (mRNA) was isolated from total RNA using the Dynabeads mRNA Purification Kit (Invitrogen, Carlsbad Ca.USA). The mRNA was fragmented and transcribed into first-strand cDNA using SuperScript(tm) II Reverse Transcriptase (Invitrogen, Carlsbad Ca. USA) and N6 primer (IDT). RNase H (Invitrogen, Carlsbad Ca. USA) and DNA polymerase I (Invitrogen, Shanghai ChinaNew England BioLabs) were subsequently applied to synthesize the second-strand cDNA. The double-stranded cDNA then underwent end-repair, a single 'A' base addition, adapter ligation, and size selection (~250bp), indexed and PCR amplification to construct library.

characteristic: description

text: adult

characteristic: life stage

text: Eupolybothrus cavernicolus

text: Eupolybothrus sp. PS-2013

text: x1 centipede

characteristic: sample amount

text: Eupolybothrus cavernicolus

text: male

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CTBE

text: public

text: UNIANDES

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

text: Chrysomelidae larvae

characteristic: host

text: Chrysomelidae larval guts in sugarcane plantations located in Ribeirao Preto, Sao Paulo State, Brazil

characteristic: isolation source

text: Pseudozyma brasiliensis

text: Pseudozyma brasiliensis

text: Pseudozyma brasiliensis sp. nov.


text: AMCNL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: metagenome

characteristic: organism

text: metagenome

text: ABcapture


text: SMU

text: public

characteristic: description

text: dense settlement biome (ENVO:01000248)

characteristic: environment (biome)

text: digestive tract (UBERON:0001555); intestine (UBERON:0000160)

characteristic: environment (feature)

text: feces (UBERON:0001988)

characteristic: environment (material)

text: Homo sapiens

characteristic: host scientific name

characteristic: host taxid

text: A

characteristic: individual

characteristic: library

text: metagenome

text: metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SMU

text: public

text: dense settlement biome (ENVO:01000248)

text: feces (UBERON:0001988)

text: Homo sapiens

text: B

text: metagenome

text: metagenome


text: SMU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: dense settlement biome (ENVO:01000248)

text: feces (UBERON:0001988)

text: Homo sapiens

text: C

text: metagenome

text: metagenome


text: SMU

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: description

text: dense settlement biome (ENVO:01000248)

text: feces (UBERON:0001988)

text: Homo sapiens

text: D

text: metagenome

characteristic: organism

text: metagenome


text: GSC:MIxS v2.1

text: GSC, MIxS, MIMS

characteristic: ENA-KEYWORD

text: Germany

text: CSISP

text: public

characteristic: age

text: ampicillin/sulbactam (2x750 mg/day) cephalosporin (3x2g/day)

characteristic: antibiotic regimen

text: stool sample

characteristic: body product

text: human gut

characteristic: body site

text: EMG

characteristic: broker name

text: November, 2009

characteristic: collection date

text: Total DNA was extracted from human fecal samples using QIAamp® DNA Stool Kit (Quiagen) following the manufacturer's instructions

text: healthy

characteristic: disease status

text: human-gut

characteristic: enviornmental package

text: Human-associated biome

characteristic: environment (biome)

text: Human-associated habitat ENVO:00009003

characteristic: environment (featurel)

text: Feces ENVO:00002003

characteristic: environment (material)

text: healthy

characteristic: gastrointestinal tract disorder

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host subject id

text: MIxS

characteristic: investigation type

text: human gut metagenome

text: BETA LACTAM ANTIBIOTICS AND HUMAN GUT MICROBIOTA

characteristic: project name

text: human gut metagenome

text: Pyrosequencing

characteristic: sequencing method

text: male

characteristic: sex

text: Day 11 Of Antibiotic Treatment Human Gut Metagenome


text: GSC:MIxS v2.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GSC, MIxS, MIMS

characteristic: ENA-KEYWORD

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Germany

text: CSISP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: antibiotic regimen

text: stool sample

characteristic: body product

text: human gut

text: EMG

text: November, 2009

characteristic: description

text: healthy

text: human-gut

text: Human-associated biome

text: Human-associated habitat ENVO:00009003

text: Feces ENVO:00002003

text: healthy

characteristic: host taxid

text: MIxS

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Pyrosequencing

text: male

text: Day 14 Of Antibiotic Treatment Human Gut Metagenome

characteristic: title


text: GSC:MIxS v2.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GSC, MIxS, MIMS

text: Germany

text: CSISP

text: public

text: stool sample

text: human gut

characteristic: body site

text: EMG

characteristic: broker name

text: November, 2009

characteristic: collection date

text: healthy

characteristic: disease status

text: human-gut

characteristic: enviornmental package

text: Human-associated biome

characteristic: environment (biome)

text: Human-associated habitat ENVO:00009003

characteristic: environment (featurel)

text: Feces ENVO:00002003

characteristic: environment (material)

text: healthy

characteristic: gastrointestinal tract disorder

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host subject id

text: MIxS

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Pyrosequencing

characteristic: sequencing method

text: male

characteristic: sex

text: Day 3 Of Antibiotic Treatment Human Gut Metagenome


text: GSC:MIxS v2.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GSC, MIxS, MIMS

characteristic: ENA-KEYWORD

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Germany

text: CSISP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: antibiotic regimen

text: stool sample

characteristic: body product

text: human gut

text: EMG

text: November, 2009

characteristic: description

text: healthy

text: human-gut

text: Human-associated biome

text: Human-associated habitat ENVO:00009003

text: Feces ENVO:00002003

text: healthy

characteristic: host taxid

text: MIxS

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Pyrosequencing

text: male

text: Day 6 Of Antibiotic Treatment Human Gut Metagenome

characteristic: title


text: GSC:MIxS v2.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GSC, MIxS, MIMS

text: Germany

text: CSISP

text: public

text: stool sample

text: human gut

characteristic: body site

text: EMG

characteristic: broker name

text: November, 2009

characteristic: collection date

text: healthy

characteristic: disease status

text: human-gut

characteristic: enviornmental package

text: Human-associated biome

characteristic: environment (biome)

text: Human-associated habitat ENVO:00009003

characteristic: environment (featurel)

text: Feces ENVO:00002003

characteristic: environment (material)

text: healthy

characteristic: gastrointestinal tract disorder

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host subject id

text: MIxS

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Pyrosequencing

characteristic: sequencing method

text: male

characteristic: sex

text: Day 0 Of Antibiotic Treatment Human Gut Metagenome


text: GSC:MIxS v2.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GSC, MIxS, MIMS

characteristic: ENA-KEYWORD

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Germany

text: CSISP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: antibiotic regimen

text: stool sample

characteristic: body product

text: human gut

text: EMG

text: November, 2009

characteristic: description

text: healthy

text: human-gut

text: Human-associated biome

text: Human-associated habitat ENVO:00009003

text: Feces ENVO:00002003

text: healthy

characteristic: host taxid

text: MIxS

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Pyrosequencing

text: male

text: Day 40 Of Antibiotic Treatment Human Gut Metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

text: BANG

text: public

text: Nodules containing adult O. ochengi parasites were obtained from the skins of cattle slaughtered at Ngaoundéré abattoir in the Adamawa Region of Cameroon by Vincent Tanya and colleagues. Adult male and female worms were separated from excised nodules; the gonads were removed from the latter (selecting only nongravid individuals) and stored in PBS or RNAlater (Sigma) at −80°C, whereas the males were preserved whole. The material was transported to the United Kingdom on dry ice and stored at −80°C. Microfilaria were collected from excised nodules. For transcriptomic analyses, total RNA was purified from the worm material stored in RNAlater using TRI Reagent (Sigma). As the nematodes in the host are closely associated with host tissues, and eat host cells, the samples contain both O. ochengi and B. indicus RNA.

text: Onchocerca ochengi

characteristic: project id

text: Onchocerca ochengi

text: Onchocerca ochengi microfilaria RNA-Seq


text: Plasmodium chabaudi

characteristic: ArrayExpress-Species

text: Plasmodium chabaudi chabaudi AS

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: T2_21_14_RNA-sc-2013-09-10T12:56:18Z-1690568

text: Plasmodium chabaudi

text: RNA extracted from purified parasites from C57 BL/6 mouse blood, timepoint 2

characteristic: sample description

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

characteristic: strain


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: T2_21_4_RNA-sc-2013-09-10T12:56:24Z-1690573

characteristic: Submitter Id

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T1_20_14_RNA-sc-2013-09-10T12:56:26Z-1690574

text: Plasmodium chabaudi

characteristic: organism

text: RNA extracted from purified parasites from C57 BL/6 mouse blood, timepoint 1

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

characteristic: ENA-CHECKLIST

text: SC

text: public

text: T1_21_1_RNA-sc-2013-09-10T12:56:27Z-1690575

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: T1_21_2_RNA-sc-2013-09-10T12:56:30Z-1690577

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T2_21_2_RNA-sc-2013-09-10T12:56:31Z-1690578

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T2_21_1_RNA-sc-2013-09-10T12:56:32Z-1690579

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T2_20_14_RNA-sc-2013-09-10T12:56:34Z-1690581

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T1_21_4_RNA-sc-2013-09-10T12:56:35Z-1690582

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS

text: SC

text: public

text: T1_21_14_RNA-sc-2013-09-10T12:56:42Z-1690588

text: Plasmodium chabaudi

text: Plasmodium chabaudi

text: Plasmodium chabaudi chabaudi AS


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: CBS 425.71

text: Geotrichum clavatum str. CBS 425.71

characteristic: title


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA11.1183


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA11.898


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA12.304


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA12.634


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA12.637


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA12.647


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA12.860


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotricum clavatum str. CNRMA8.1479


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA10.623


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA12.494


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA12.559


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA12.615


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA12.657


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA12.667


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA6.1177


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA8.1167


text: IP

text: public

text: Saprochaete clavata

text: Saprochaete clavata

text: Geotrichum clavatum str. CNRMA9.356


text: NCSU

text: public

text: AGAGTTTGATCCTGGCTCAG

characteristic: adapters

unit: week

characteristic: age

text: Jejunem

characteristic: body site

characteristic: collection date

text: controlled

characteristic: environment (biome)

text: indoor

characteristic: environment (feature)

text: pens

characteristic: environment (material)

text: USA

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: chicken

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: Line-HAS

characteristic: miscellaneous parameter

text: ACGAGTGCGT

characteristic: multiplex identifiers

text: metagenome

characteristic: project name

text: metagenome

text: pyrosequencing

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERISTY OF OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERISTY OF OXFORD

characteristic: Submitter Id

text: Peptoclostridium difficile

characteristic: organism

text: Peptoclostridium difficile

characteristic: title


text: UNIVERISTY OF OXFORD

text: public

text: serial paired sample

characteristic: description

text: Peptoclostridium difficile

text: Peptoclostridium difficile


text: Chlamydia trachomatis

characteristic: ArrayExpress-Species

text: SC

text: public

text: Chlamydia trachomatis L2b/UCH-1/proctitis


text: SMU

text: public

text: BV

characteristic: disease

text: metagenome

characteristic: ph

text: metagenome

text: Disease

characteristic: status


characteristic: ENA-CHECKLIST

text: SMU

text: public

text: BV

text: metagenome

text: metagenome

text: Recovery


characteristic: ENA-FIRST-PUBLIC

text: SMU

text: public

text: BV.VVC

text: metagenome

text: metagenome

text: Disease


characteristic: ENA-LAST-UPDATE

text: SMU

text: public

text: BV.VVC

text: metagenome

text: metagenome

text: Recovery


characteristic: External Id

text: SMU

text: public

text: VVC

text: metagenome

text: metagenome

text: Disease


text: SMU

characteristic: INSDC center name

text: public

text: VVC

text: metagenome

text: metagenome

text: Recovery


text: SMU

characteristic: INSDC first public

text: public

text: metagenome

text: metagenome

text: Healthy


text: GSC

characteristic: INSDC last update

text: public

text: Station23 - Deep Chlorophyl Maximum - 180-2000 µm - DNA

text: marine metagenome

text: marine metagenome

text: Shotgun Sequencing of Tara Oceans DNA samples corresponding to size fractions for protist


text: GSC

text: public

characteristic: INSDC status

text: Station23 - Surface - 5-20 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

characteristic: SRA accession

text: Station30 - Deep Chlorophyl Maximum - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

characteristic: Submitter Id

text: Station23 - Surface - 180-2000 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station30 - Surface - 180-2000 µm - DNA

text: marine metagenome

characteristic: organism

text: marine metagenome


text: GSC

text: public

text: Station30 - Deep Chlorophyl Maximum - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome

characteristic: title


text: GSC

text: public

text: Station30 - Surface - 5-20 µm - DNA

characteristic: description

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Deep Chlorophyl Maximum - 5-20 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station30 - Surface - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Surface - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Surface - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station30 - Deep Chlorophyl Maximum - 5-20 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Deep Chlorophyl Maximum - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station30 - Surface - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Deep Chlorophyl Maximum - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Surface - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome

text: Shotgun Sequencing of Tara Oceans DNA samples corresponding to size fractions for protists


text: GSC

text: public

text: Station7 - Deep Chlorophyl Maximum - 5-20 µm - WGA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Deep Chlorophyl Maximum - 20-180 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Surface - 5-20 µm - WGA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Deep Chlorophyl Maximum - 180-2000 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Surface - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Surface - 180-2000 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Deep Chlorophyl Maximum - 0.8-5 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station30 - Deep Chlorophyl Maximum - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome

text: Shotgun Sequencing of Tara Oceans DNA samples corresponding to size fractions for prokaryotes and large DNA viruses


text: GSC

text: public

text: Station7 - Surface - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome

text: 16S rDNA Gene Sequencing of Tara Oceans DNA samples corresponding to size fractions for prokaryotes


text: GSC

text: public

text: Station30 - Surface - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Deep Chlorophyl Maximum - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station7 - Deep Chlorophyl Maximum - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome


text: GSC

text: public

text: Station23 - Surface - 0.2-1.6 µm - DNA

text: marine metagenome

text: marine metagenome


text: INRA-CMSE

text: public

characteristic: Soil Fraction class (µm)

characteristic: collection date

text: no till cropland

characteristic: current land use

unit: m

characteristic: depth

text: this sample is the microenvironments corresponding to the fine silt (<20;>2µm) fraction obtained after a fractionation procedure under no till condition

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CATACTCTAC

characteristic: multiplex identifiers

text: DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012). Briefly, in a 15 ml Falcon tube 1 g of each soil sample was mixed with 4 ml of a solution containing 100 mM Tris (pH 8.0), 100 mM EDTA (pH 8.0), 100 mM NaCl, and 2% (wt/vol) sodium dodecylsulphate. 2 g of 100 µm diameter silica beads, 2.5 g of 1.4 mm diameter ceramic beads and 4 glass beads of 4 mm diameter were added to the mixture. The samples were then homogenized for 3 x 30 s at 4 m sec-1 in a FastPrep®-24 (MP-Biomedicals, NY, USA) and incubated for 30 min at 70°C before centrifugation at 7000 x g for 5 min at 20°C. For the deproteination, 1 ml of the collected supernatants was incubated for 10 min on ice with 1/10 volume of 3 M potassium acetate (pH 5.5) and centrifuged at 14000 x g for 5 min. Finally, after precipitation with one volume of ice-cold isopropanol, the nucleic acids were washed with 70% ethanol. For purification, DNA extracts were loaded onto PVPP (polyvinyl polypyrrolidone) minicolumns (BIORAD, Marne la Coquette, France) and centrifuged at 1000 x g for 2 min at 10°C. The collected eluate was then purified with the Geneclean Turbo (Q Biogene®, IllKirch, France).

characteristic: nucleic acid extraction

text: soil metagenome

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation:94degC_2.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases

characteristic: pcr conditions

text: F479:5-CAGCMGCYGCNGTAANAC-3; R888:5-CCGYCAATTCMTTTRAGT-3

characteristic: pcr primers

characteristic: project name

unit: g

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

unit: g/kg

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: no till cropland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the fine sand (<250;>63µm) fraction obtained after a fractionation procedure under no till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CGTAGACTAG

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: no till cropland

text: this sample is the microenvironments corresponding to the coarse silt (<63;>20µm) fraction obtained after a fractionation procedure under no till condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CAGTAGACGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: BulkSoil

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: no till cropland

characteristic: current land use

characteristic: depth

text: this sample is the bulk soil under no till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CACACGATAG

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: till cropland

text: this sample is the microenvironments corresponding to the indispersible clay (<2µm) fraction obtained after a fractionation procedure under till condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGCGTATACA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: till cropland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the coarse sand (<2000;>250µm) fraction obtained after a fractionation procedure under till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CGTGTCTCTA

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: till cropland

text: this sample is the microenvironments corresponding to the fine silt (<20;>2µm) fraction obtained after a fractionation procedure under till condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACACGATAG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: till cropland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the fine sand (<250;>63µm) fraction obtained after a fractionation procedure under till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CGTCTAGTAC

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: till cropland

text: this sample is the microenvironments corresponding to the coarse silt (<63;>20µm) fraction obtained after a fractionation procedure under till condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: BulkSoil

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: till cropland

characteristic: current land use

characteristic: depth

text: this sample is the bulk soil under till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CATACTCTAC

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: grassland

text: this sample is the microenvironments corresponding to the indispersible clay (<2µm) fraction obtained after a fractionation procedure under grassland condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: grassland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the coarse sand (<2000;>250µm) fraction obtained after a fractionation procedure under grassland condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CGTGTCTCTA

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: grassland

text: this sample is the microenvironments corresponding to the fine silt (<20;>2µm) fraction obtained after a fractionation procedure under grassland condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACACGATAG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: grassland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the fine sand (<250;>63µm) fraction obtained after a fractionation procedure under grassland condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CATAGTAGTG

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: grassland

text: this sample is the microenvironments corresponding to the coarse silt (<63;>20µm) fraction obtained after a fractionation procedure under grassland condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTCTAGTAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: BulkSoil

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: grassland

characteristic: current land use

characteristic: depth

text: this sample is the bulk soil under grassland condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CGATCGTATA

text: DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012). Briefly, in a 15 ml Falcon tube 1 g of each soil sample was mixed with 4 ml of a solution containing 100 mM Tris (pH 8.0), 100 mM EDTA (pH 8.0), 100 mM NaCl, and 2% (wt/vol) sodium dodecylsulphate. 2 g of 100 µm diameter silica beads, 2.5 g of 1.4 mm diameter ceramic beads and 4 glass beads of 4 mm diameter were added to the mixture. The samples were then homogenized for 3 x 30 s at 4 m sec-1 in a FastPrep®-24 (MP-Biomedicals, NY, USA) and incubated for 30 min at 70°C before centrifugation at 7000 x g for 5 min at 20°C. For the deproteination, 1 ml of the collected supernatants was incubated for 10 min on ice with 1/10 volume of 3 M potassium acetate (pH 5.5) and centrifuged at 14000 x g for 5 min. Finally, after precipitation with one volume of ice-cold isopropanol, the nucleic acids were washed with 70% ethanol. For purification, DNA extracts were loaded onto PVPP (polyvinyl polypyrrolidone) minicolumns (BIORAD, Marne la Coquette, France) and centrifuged at 1000 x g for 2 min at 10°C. The collected eluate was then purified with the Geneclean Turbo (Q Biogene®, IllKirch, France).

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

text: no till cropland

text: this sample is the microenvironments corresponding to the indispersible clay (<2µm) fraction obtained after a fractionation procedure under no till condition

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation:94degC_2.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases

characteristic: pcr conditions

text: F479:5-CAGCMGCYGCNGTAANAC-3; R888:5-CCGYCAATTCMTTTRAGT-3

characteristic: pcr primers

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Soil Fraction class (µm)

characteristic: Submitter Id

characteristic: collection date

text: no till cropland

characteristic: current land use

characteristic: depth

text: this sample is the microenvironments corresponding to the coarse sand (<2000;>250µm) fraction obtained after a fractionation procedure under no till condition

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

text: metagenome

text: CATAGTAGTG

text: soil metagenome

characteristic: organism

characteristic: ph

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

unit: L

characteristic: amount or size of sample collected

text: Vineyard with conventional treatment.

text: Grapes come from a unique plot divided in 3 equal parts situated in Burgundy. Each part of the plot was managed since 2006 using 3 different farming methods referred to as “Conventional”, “Organic”, “Ecophyto”.The conventional part of vineyard was treated with chemicals, while the “ecophyto” modality was treated with the same products than the conventional but half the dose. The “Organic” modality did not use any chemical product but only copper and sulphur. The same amount of grapes was collected (3Kg) in aseptic conditions and placed in sterile bags. Grapes were squeezed by hand in the plastic bags, 250 mL of must were poured into 500 mL sterile fermenters. Samples were taken and collected at beginning and middle of fermentation, and at the end of the alcoholic fermentation.

text: Chardonnay grapes

text: plant-associated habitat

text: environmental material

text: miscellaneous natural or artificial environment

characteristic: environmental package

text: France, Burgundy, Chardonnay

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGACGTGACT

characteristic: multiplex identifiers

text: metagenome

text: initial denaturation:94degC_3.0min;35cycles of denaturation:94degC_1.0min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min

characteristic: pcr conditions

text: FR1:ANCCATTCAATCGGTANT; FF390:CGATAACGAACGAGACCT

characteristic: pcr primers

characteristic: project name

text: metagenome

text: pyrosequencing

characteristic: sequencing method

text: 18S rRNA

characteristic: target gene

text: Fungal biodiversity of Chardonnay grapes after conventional treatment, at the beginning of the fermentation.


characteristic: ENA-CHECKLIST

text: SRA-Webin

text: INRA-CMSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Vineyard with conventional treatment.

characteristic: current land use

characteristic: description

text: Chardonnay grapes

characteristic: environment (biome)

text: plant-associated habitat

characteristic: environment (feature)

text: environmental material

characteristic: environment (material)

text: France, Burgundy, Chardonnay

text: metagenome

text: CGCGTATACA

text: metagenome

characteristic: organism

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after conventional treatment, at the middle of the fermentation.

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

characteristic: amount or size of sample collected

text: Vineyard with conventional treatment.

text: Chardonnay grapes

text: plant-associated habitat

text: environmental material

characteristic: environmental package

text: France, Burgundy, Chardonnay

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

text: metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after conventional treatment, at the end of the alcoholic fermentation.


characteristic: ENA-CHECKLIST

text: SRA-Webin

text: INRA-CMSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Vineyard with organic treatment.

characteristic: current land use

characteristic: description

text: Chardonnay grapes

characteristic: environment (biome)

text: plant-associated habitat

characteristic: environment (feature)

text: environmental material

characteristic: environment (material)

text: France, Burgundy, Chardonnay

text: metagenome

text: CGTCTAGTAC

text: metagenome

text: metagenome

text: pyrosequencing

characteristic: sequencing method

text: 18S rRNA

characteristic: target gene

text: Fungal biodiversity of Chardonnay grapes after organic treatment (any use of chemical product but only copper and sulphur), at the beginning of the fermentation.


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

characteristic: amount or size of sample collected

text: Vineyard with organic treatment.

text: Chardonnay grapes

text: plant-associated habitat

text: environmental material

characteristic: environmental package

text: France, Burgundy, Chardonnay

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

text: metagenome

characteristic: organism

characteristic: pcr conditions

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after organic treatment (any use of chemical product but only copper and sulphur), at the middle of the fermentation.

characteristic: title


characteristic: ENA-CHECKLIST

text: SRA-Webin

text: INRA-CMSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Vineyard with organic treatment.

characteristic: current land use

characteristic: description

text: Chardonnay grapes

characteristic: environment (biome)

text: plant-associated habitat

characteristic: environment (feature)

text: environmental material

characteristic: environment (material)

text: France, Burgundy, Chardonnay

text: metagenome

text: CAGTAGACGT

text: metagenome

characteristic: pcr primers

characteristic: project name

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after organic treatment (any use of chemical product but only copper and sulphur), at the end of the alcoholic fermentation.


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

characteristic: amount or size of sample collected

text: Vineyard with ecophyto treatment.

text: Chardonnay grapes

text: plant-associated habitat

text: environmental material

characteristic: environmental package

text: France, Burgundy, Chardonnay

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACGTGTCGC

characteristic: multiplex identifiers

text: metagenome

characteristic: organism

text: metagenome

text: pyrosequencing

characteristic: sequencing method

text: 18S rRNA

characteristic: target gene

text: Fungal biodiversity of Chardonnay grapes after ecophyto treatment (half doses compared to the conventional), at the beginning of the fermentation.


characteristic: ENA-CHECKLIST

text: SRA-Webin

text: INRA-CMSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Vineyard with ecophyto treatment.

characteristic: current land use

characteristic: description

text: Chardonnay grapes

characteristic: environment (biome)

text: plant-associated habitat

characteristic: environment (feature)

text: environmental material

characteristic: environment (material)

text: France, Burgundy, Chardonnay

text: metagenome

text: CATACTCTAC

text: metagenome

characteristic: pcr conditions

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after ecophyto treatment (half doses compared to the conventional), at the middle of the fermentation.

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SRA-Webin

characteristic: ENA-SUBMISSION-TOOL

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

text: public

characteristic: amount or size of sample collected

text: Vineyard with ecophyto treatment.

text: Chardonnay grapes

text: plant-associated habitat

text: environmental material

characteristic: environmental package

text: France, Burgundy, Chardonnay

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGACACTATC

characteristic: multiplex identifiers

text: metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: metagenome

text: pyrosequencing

text: 18S rRNA

text: Fungal biodiversity of Chardonnay grapes after ecophyto treatment (half doses compared to the conventional), at the end of the alcoholic fermentation.


characteristic: ENA-CHECKLIST

text: INRA-CMSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: the sample is obtained from 20 g of a soil that has been sterilized by gamma radiation (35 KGy; Conservatome, Dagneux, France) and then placed in 150-ml hermetically sealed plasma flasks. Soil were inoculated with dilution of the same soil suspension (not sterilized)

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CGTACAGTCA

text: For each microcosm, DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012). Briefly, in a 15 ml Falcon tube 2 g of each soil sample was mixed with 8 ml of a solution containing 100 mM Tris (pH 8.0), 100 mM EDTA (pH 8.0), 100 mM NaCl, and 2% (wt/vol) sodium dodecylsulphate. 4 g of 100 µm diameter silica beads, 5 g of 1.4 mm diameter ceramic beads and 8 glass beads of 4 mm diameter were added to the mixture. The samples were then homogenized for 3 x 30 s at 4 m sec-1 in a FastPrep®-24 (MP-Biomedicals, NY, USA) and incubated for 30 min at 70°C before centrifugation at 7000 x g for 5 min at 20°C. For the deproteination, 1 ml of the collected supernatants was incubated for 10 min on ice with 1/10 volume of 3 M potassium acetate (pH 5.5) and centrifuged at 14000 x g for 5 min. Finally, after precipitation with one volume of ice-cold isopropanol, the nucleic acids were washed with 70% ethanol. For purification, DNA extracts were loaded onto PVPP (polyvinyl polypyrrolidone) minicolumns (BIORAD, Marne la Coquette, France) and centrifuged at 1000 x g for 2 min at 10°C. The collected eluate was then purified with the Geneclean Turbo (Q Biogene®, IllKirch, France).

characteristic: nucleic acid extraction

text: soil metagenome

text: 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_2.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5’ position to specifically identify each sample and avoid PCR biases

text: F479:5’-CAGCMGCYGCNGTAANAC-3’; R888:5’-CCGYCAATTCMTTTRAGT-3’

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

text: Microbial diversity: an insurance for the stability of soil functioning ?

characteristic: total n

characteristic: total organic carbon

unit: g/g

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CGATCGTATA

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACGTAGATC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CACGTGTCGC

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGACGTGACT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CATACTCTAC

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGCGTATACA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CAGTAGACGT

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTCTAGTAC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

text: 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min; 35 cycles of denaturation : 94degC_1.0min; annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5’ position to specifically identify each sample and avoid PCR biases

characteristic: pcr conditions

text: FR1:5’-ANCCATTCAATCGGTANT-3’;FF390:5’-CGATAACGAACGAGACCT-3’

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CGAGACGCGC

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CATAGTGACA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CAGACGTCTG

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

characteristic: Pre-incubation temperature (°C)

characteristic: SRA accession

characteristic: Soil dilution used for inoculation

characteristic: Submitter Id

characteristic: collection date

text: Pasture

characteristic: current land use

characteristic: depth

text: temperate grasslands

text: field soil

text: cambisol

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCTGTCG

characteristic: multiplex identifiers

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: Luvisol

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Pre-incubation duration (days)

text: Pasture

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: cambisol

characteristic: environment (material)

text: France

characteristic: geographic location (elevation)

text: mimarks-survey

text: CAGTAGACGT

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: ph

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Luvisol

characteristic: soil type

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: total n

characteristic: total organic carbon

characteristic: water content of soil