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text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 334, HS batch 4, operator H, extraction robot F, sequencer B

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 335, HS batch 4, operator E, extraction robot I, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 336, HS batch 4, operator E, extraction robot G, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 337, HS batch 4, operator E, extraction robot H, sequencer A

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 338, HS batch 4, operator H, extraction robot C, sequencer B

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 339, HS batch 4, operator M, extraction robot D, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 340, HS batch 4, operator M, extraction robot F, sequencer A

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 341, HS batch 4, operator E, extraction robot I, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 342, HS batch 4, operator H, extraction robot D, sequencer B

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

text: 16s RNA gene sequencing of clinically relevant microbial taxa from the human gut

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 343, HS batch 4, operator H, extraction robot F, sequencer B

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 344, HS batch 4, operator H, extraction robot H, sequencer B

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 345, HS batch 4, operator M, extraction robot G, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 346, HS batch 4, operator G, extraction robot H, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 347, HS batch 4, operator M, extraction robot G, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 348, HS batch 4, operator M, extraction robot H, sequencer A

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 349, HS batch 4, operator G, extraction robot D, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 350, HS batch 4, operator G, extraction robot F, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 351, HS batch 4, operator D, extraction robot F, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 352, HS batch 4, operator G, extraction robot A, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 353, HS batch 4, operator G, extraction robot C, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 354, HS batch 4, operator D, extraction robot D, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 355, HS batch 4, operator D, extraction robot H, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 356, HS batch 4, operator D, extraction robot F, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 357, HS batch 4, operator M, extraction robot F, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 358, HS batch 4, operator M, extraction robot H, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 359, HS batch 4, operator M, extraction robot I, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: 16s RNA gene sequencing of clinically relevant microbial taxa from the human gut

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 360, HS batch 4, operator M, extraction robot D, sequencer C

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: uBiome

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 361, HS batch 4, operator M, extraction robot G, sequencer C

characteristic: description

text: human-associated habitat

characteristic: environment (biome)

text: intestine environment

characteristic: environment (feature)

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: uBiome

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 362, HS batch 4, operator L, extraction robot H, sequencer B

text: human-associated habitat

text: intestine environment

text: feces

text: USA

text: human-gut

text: mimarks-survey

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: uBiome

text: public

text: Set of 363 homogenized stool sample replicates, Subject_B, feces, replicate 363, HS batch 4, operator L, extraction robot F, sequencer B

text: human-associated habitat

text: intestine environment

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: human gut metagenome

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: Submitter Id

characteristic: collection date

text: seawater metagenome

characteristic: common name

characteristic: depth

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environmental package

text: Pacific Ocean

text: metagenome

text: seawater metagenome

characteristic: organism

text: seawater metagenome

text: PE Illumina Miseq 2x300

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: seawater metagenome

characteristic: environment (material)

text: water

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x301


text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: seawater metagenome

characteristic: common name

text: water

text: Pacific Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: seawater metagenome

characteristic: project name

text: seawater metagenome

text: PE Illumina Miseq 2x302

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: seawater metagenome

characteristic: depth

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environmental package

text: Pacific Ocean

text: metagenome

text: seawater metagenome

characteristic: organism

text: seawater metagenome

text: PE Illumina Miseq 2x303

characteristic: title


characteristic: External Id

text: UNIVERSITY OF SOUTHERN CALIFORNIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: seawater metagenome

characteristic: environment (material)

text: water

text: Pacific Ocean

characteristic: geographic location (country and/or sea)

text: seawater metagenome

text: seawater metagenome

text: PE Illumina Miseq 2x300


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: unidentified

text: unidentified


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF SOUTHERN CALIFORNIA

text: public

text: Blank library

characteristic: description

text: unidentified

text: unidentified


text: UNIVERSITY OF SHEFFIELD

text: public

tag: Namespace:UNIVERSITY OF SHEFFIELD

text: thale cress

characteristic: common name

text: pooled sample from 10-12 leaves from atleast 5 plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: epiRILS-RNA-seq


text: UNIVERSITY OF SHEFFIELD

text: public

text: thale cress

text: pooled sample form 10 -12 similar aged leaves from 5 plants atleast

text: Columbia

characteristic: ecotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaves

characteristic: tissue type

text: epiRIls - WGBS


text: public

tag: Namespace:Statistical Genomics group Institute of Molecular Life Sciences

characteristic: amplicon

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: Transfected cells were collected by trypsinization and were washed with PBS. HEK293T cells were obtained from ATCC and verified mycoplasma free (GATC Biotech). The HEK293T reporter line was maintained in DMEM with GlutaMax (Gibco). Media was supplemented with 10% FBS (Sigma), and 100 μg/mL Penicillin-Streptomycin (Gibco). K562 cells were obtained from Sigma Aldrich, verified mycoplasma free and were maintained in RPMI 1640 medium with GlutaMax. Additional the medium supplemented with 10% FBS, and 100 μg/mL Penicillin-Streptomycin. Cells were passaged three times per week. Cells were grown at 37C in a humidified 5% CO2 environment. WT E14 mESC line (ATCC CRL-1821) was cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma-Aldrich), containing 15% of fetal bovine serum (FBS; Life Technologies), 100 U/mL LIF (Millipore), 0.1 mM 2-ß-mercaptoethanol (Life Technologies) and 1% Penicillin/Streptomycin (Gibco), on 0.2% gelatin-coated support in absence of feeder cells. The culture medium was changed daily. HEK293T cells were seeded in 24-well plates at 120.000 - 140.000 cells per well, 1 day prior to transfection. K562 cells were 6h prior to transfection distributed in 24 well plates at a density of 220.000- 240.000 cells per well. On the day of transfection, RNP and RNPD complexes (2.2 pmols) were complexed with sgRNA (3.88 pmols) in Opti-MEM (Invitrogen) and briefly vortexed, followed by adding 3 μl the Lipofectamine 2000 reagent (Invitrogen) with Opti-MEM. The resulting mixture was incubated for 15 min at room temperature to allow lipid particle formation. After 15 minutes of incubation at room temperature, the mixture was dropped slowly into the well. One day post-transfection, cells were transferred to an 10cm dish. mESCs were transfected into 6-well plate using Lipofectamine 2000. Cells were plated 24 hours before transfection at a density of 20,000 cells/cm2 per well and cultured in culture medium without streptomycin and penicillin. The medium was changed to mESC culture medium 8 hours after transfection. Cells were collected 48h post-transfection. The expression of SpCas9-SNAP protein necessary for RNP/RNPD complexes was done from bacterial expression vector pNS20-SpCas9-SNAP. Wild Type Streptococcus pyogenes Cas9 (SpCas9 WT) proteins were expressed in Escherichia coli BL21 (DE3) Rosetta 2 from plasmid pMJ806. Bacterial expression plasmid pMJ806 was a gift from Jennifer Doudna (Addgene plasmid # 39312). In the RNP sample the system contains SpCas9-SNAP protein complexed with the sgRNA, but no repair oligo. PBS was discarded and DNA extraction was preformed using DNeasy Blood and Tissue kit (Qiagen) following manufacturer’s protocol. The PCR amplicons flanking the targeted site were generated using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). PCR cycling conditions used were as follows: 1x 98°C for 3 min; 27x 95°C for 15 s, 65°C for 15 s, 72°C for 30 s; 1x 72°C for 5 min. Annealing temperature was optimized for each primer set to ensure that a single amplicon was produced. PCR amplicons were purified by solid phase reversible immobilization (SPRI) bead cleanup using Agencourt AMPure XP reagent (#A63881, Beckmann-Coulter, Indianapolis, IN, USA), per the manufacturer’s instructions. For the generation of the pooled sequencing libraries, the TruSeq (Illumina®) Index Adaptor Sequences were added at the second amplification step. The resulting Illumina libraries with Index Adaptors were purified with AMPure XP reagent. Quality control for the final library was performed using the High sensitivity D1000 ScreenTape at Agilent 2200 TapeStation.

text: no treatment

characteristic: experimental condition

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: replicate

text: Homo sapiens

characteristic: title


characteristic: External Id

text: Statistical Genomics group Institute of Molecular Life Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: no treatment

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: RNP + 65mer uncoupled (SpCas9 WT)

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

characteristic: amplicon

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: RNPD + 65mer coupled (SpCas9-SNAP)

characteristic: experimental condition

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

text: hHBB

text: ArrayExpress

text: human

text: no treatment

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: replicate

text: Homo sapiens

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: hHBB

text: ArrayExpress

text: human

text: RNP + 65mer uncoupled (SpCas9 WT)

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

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text: Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the ‘brain’) and hindbrain (referred to as the ‘cerebellum’). The postnatal ‘brain’ and ‘cerebellum’ samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).

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text: E-MTAB-6813:5453sTS.Macaque.Kidney.1dpb.Male

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text: Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the ‘brain’) and hindbrain (referred to as the ‘cerebellum’). The postnatal ‘brain’ and ‘cerebellum’ samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).

text: postnatal

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text: Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the ‘brain’) and hindbrain (referred to as the ‘cerebellum’). The postnatal ‘brain’ and ‘cerebellum’ samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).

text: postnatal

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characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6071sTS.Macaque.Kidney.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:6074sTS.Macaque.Kidney.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:6124sTS.Macaque.Kidney.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:5366sTS.Macaque.Liver.1ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5497sTS.Macaque.Liver.1ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6069sTS.Macaque.Liver.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:6073sTS.Macaque.Liver.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:5418sTS.Macaque.Testis.1ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:5451sTS.Macaque.Testis.1ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6075sTS.Macaque.Testis.1ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6109sTS.Macaque.Brain.2ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: forebrain

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:6076sTS.Macaque.Brain.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: forebrain

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:6084sTS.Macaque.Brain.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the ‘brain’) and hindbrain (referred to as the ‘cerebellum’). The postnatal ‘brain’ and ‘cerebellum’ samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: forebrain

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:5422sTS.Macaque.Heart.3ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5436sTS.Macaque.Heart.3ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6078sTS.Macaque.Heart.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:6081sTS.Macaque.Heart.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:5428sTS.Macaque.Cerebellum.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: hindbrain

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:5442sTS.Macaque.Cerebellum.3ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: hindbrain

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5380sTS.Macaque.Kidney.3ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5507sTS.Macaque.Kidney.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:6079sTS.Macaque.Kidney.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:6083sTS.Macaque.Kidney.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: kidney

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:5447sTS.Macaque.Liver.3ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5454sTS.Macaque.Liver.3ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6077sTS.Macaque.Liver.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: liver

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:5440sTS.Macaque.Testis.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:5443sTS.Macaque.Testis.3ypb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:6080sTS.Macaque.Testis.3ypb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6082sTS.Macaque.Testis.3ypb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

text: Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the ‘brain’) and hindbrain (referred to as the ‘cerebellum’). The postnatal ‘brain’ and ‘cerebellum’ samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: testis

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:5434sTS.Macaque.Brain.151dpb.Male

unit: month

text: ArrayExpress

text: Rhesus monkey

text: postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Macaca mulatta

text: Macaca mulatta

text: forebrain

characteristic: organism part

text: Macaca mulatta

text: male

characteristic: sex


characteristic: External Id

text: University of Heidelberg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6813:5494sTS.Macaque.Brain.153dpb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: forebrain

text: Macaca mulatta

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Heidelberg

text: public

text: E-MTAB-6813:5438sTS.Macaque.Heart.153dpb.Male

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

characteristic: organism

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Heidelberg

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6813:6060sTS.Macaque.Heart.6mpb.Male

characteristic: Submitter Id

text: ArrayExpress

text: Rhesus monkey

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male


text: University of Heidelberg

text: public

text: E-MTAB-6813:6065sTS.Macaque.Heart.6mpb.Male

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Rhesus monkey

characteristic: common name

characteristic: description

text: postnatal

text: wild type genotype

text: Macaca mulatta

text: Macaca mulatta

text: heart

text: Macaca mulatta

text: male