E-MTAB-7098:Sample 432

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 432

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 432


E-MTAB-7098:Sample 433

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 433

tag: Namespace:Karolinska Institutet, Department of Cell and Molecular Biology

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Primary fibroblasts were derived from adult CAST/EiJ x C57/Bl6 mice. Tail explants were cultured in fibroblast medium (DMEM high glucose, 10% ES cell FBS, 1% Penicillin/Streptomycin, 1% non-essential amino acids, 1% sodium-puryvate, 0.1mM b-Mercaptoethanol). Cells were passaged twice before distributed as individual cells into wells of 384 or 96 well plates using a FACS. Wells was pre-loaded with lysis buffer. ES cells were cultured as in Meissner et al. Methods Mol Biol 2009, and similarly distributed via FACS into as single cells into wells of 96 and 384-well plates Cell lysis. Smart-seq2, indexed by Nextera i5 and i7 and with a unique molecular identifier present in template-switching oligonucleotide. No exogenous spike-ins were used..

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 433

characteristic: title


E-MTAB-7098:Sample 434

text: Karolinska Institutet, Department of Cell and Molecular Biology

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 434

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 434


E-MTAB-7098:Sample 435

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 435

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 435


E-MTAB-7098:Sample 436

text: public

text: E-MTAB-7098:Sample 436

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 436


E-MTAB-7098:Sample 437

text: public

text: E-MTAB-7098:Sample 437

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 437


E-MTAB-7098:Sample 438

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 438

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 438

characteristic: title


E-MTAB-7098:Sample 439

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 439

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 439


E-MTAB-7098:Sample 440

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 440

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 440


E-MTAB-7098:Sample 441

text: public

text: E-MTAB-7098:Sample 441

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 441


E-MTAB-7098:Sample 442

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 442

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 442


E-MTAB-7098:Sample 443

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 443

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 443

characteristic: title


E-MTAB-7098:Sample 444

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 444

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 444


E-MTAB-7098:Sample 445

text: public

text: E-MTAB-7098:Sample 445

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 445


E-MTAB-7098:Sample 446

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 446

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 446


E-MTAB-7098:Sample 447

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 447

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 447


E-MTAB-7098:Sample 448

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 448

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 448

characteristic: title


E-MTAB-7098:Sample 449

text: public

text: E-MTAB-7098:Sample 449

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 449


E-MTAB-7098:Sample 450

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 450

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 450


E-MTAB-7098:Sample 451

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 451

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 451


E-MTAB-7098:Sample 452

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 452

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 452


E-MTAB-7098:Sample 453

text: public

text: E-MTAB-7098:Sample 453

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 453

characteristic: title


E-MTAB-7098:Sample 454

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 454

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 454


E-MTAB-7098:Sample 455

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 455

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 455


E-MTAB-7098:Sample 456

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 456

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 456


E-MTAB-7098:Sample 457

text: public

text: E-MTAB-7098:Sample 457

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 457


E-MTAB-7098:Sample 458

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 458

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 458

characteristic: title


E-MTAB-7098:Sample 459

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 459

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 459


E-MTAB-7098:Sample 460

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 460

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 460


E-MTAB-7098:Sample 461

text: public

text: E-MTAB-7098:Sample 461

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 461


E-MTAB-7098:Sample 462

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 462

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 462


E-MTAB-7098:Sample 463

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 463

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 463

characteristic: title


E-MTAB-7098:Sample 464

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 464

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 464


E-MTAB-7098:Sample 465

text: public

text: E-MTAB-7098:Sample 465

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 465


E-MTAB-7098:Sample 466

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 466

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 466


E-MTAB-7098:Sample 467

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 467

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 467


E-MTAB-7098:Sample 468

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 468

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 468

characteristic: title


E-MTAB-7098:Sample 469

text: public

text: E-MTAB-7098:Sample 469

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 469


E-MTAB-7098:Sample 470

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 470

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 470


E-MTAB-7098:Sample 471

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 471

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 471


E-MTAB-7098:Sample 472

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 472

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 472


E-MTAB-7098:Sample 473

text: public

text: E-MTAB-7098:Sample 473

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 473

characteristic: title


E-MTAB-7098:Sample 474

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 474

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 474


E-MTAB-7098:Sample 475

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 475

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 475


E-MTAB-7098:Sample 476

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 476

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x CAST/EiJ

text: Sample 476


E-MTAB-7098:Sample 477

text: public

text: E-MTAB-7098:Sample 477

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

characteristic: strain

text: Sample 477


E-MTAB-7098:Sample 478

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7098:Sample 478

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 478

characteristic: title


E-MTAB-7098:Sample 479

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-7098:Sample 479

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 479


E-MTAB-7098:Sample 480

characteristic: INSDC first public

text: public

text: E-MTAB-7098:Sample 480

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: male

text: C57BL/6 x CAST/EiJ

text: Sample 480


text: INSERM

text: public

text: ArrayExpress

text: CiGEnC

characteristic: cell line

text: microvascular endothelial cell

text: human

characteristic: common name

text: Protocols: Total RNAs were isolated from 3 replicates of the CiGEnC cells and from banked primary macrovascular ECs obtained in 5 donors using an RNeasy Kit (Qiagen) including a DNase treatment step. Total RNAs were isolated using the RNeasy Kit (QIAGEN) including a DNAse treatment step. RNA quality was assessed using RNA Screen Tape 6000 Pico LabChips with the Tape Station (Agilent Technologies) and the RNA concentration was measured by spectrophometry using the Xpose (Trinean). RNAseq libraries were prepared starting from 2 µg of total RNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as recommended by the manufacturer.

characteristic: description

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: INSERM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: HEAC

text: macrovascular endothelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: aorta

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: University of Portsmouth

text: public

characteristic: SRA accession

tag: Namespace:University of Portsmouth

text: ArrayExpress

text: baker's yeast

text: Protocols: For each strain, three independent colonies were used to prepare samples of total RNA. Wild-type H4 and otherwise isogenic T80A H4 cells were grown in yeast extract, peptone, glucose, adenine sulfate (YPDA) to an optical density at 660 nm (OD660) of 0.6. Total RNA was isolated through hot-phenol extraction. RNA was further subjected to DNase I treatment (catalog number E1009-A; Zymo Research) and poly-A mRNA was purified using poly-T oligo-attached magnetic beads (NEXTflex Poly(A) Beads, catalog number NOVA-512981) according to the manufacturer's instructions. The quantity and purity of RNA were measured by using an Agilent high-sensitivity RNA screen tape system (catalog number 5067-5579; Agilent Technologies) and Qubit (Molecular Probes, Invitrogen). Libraries were prepared for sequencing from 200 ng of polyA mRNA by using the NEXTflex RNA-seq kit (NOVA-512913, Illumina) according to the manufacturer's instructions. Samples were barcoded and combined at uniform molarity to create a single pool for sequencing.

characteristic: genotype

text: YPDA medium

characteristic: growth condition

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Portsmouth

characteristic: INSDC first public

text: public

text: ArrayExpress

text: baker's yeast

text: wild type genotype

text: YPDA medium

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

tag: Namespace:Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris Sud, University Paris Saclay, F-91198 Gif-sur-Yvette cedex, France;

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: The cell pellets are resuspended in 1 ml of FA/SDS/PMSF buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF) and transferred to 1.5 mL Eppendorf tubes. 0.75 mL glass beads (425-600 µm, Sigma) are then added to each tube, and the yeast cells were lysed at 4°C for 15 min by vortexing. Pierce the bottom of a 12 ml Greiner tube with a hot needle (0.7x30 mm or 0.5x15 mm) and place in a Falcon50 with a pierced cap. The lysates are transfered in the pierced tubes. Wash each Epp with twice 1 ml of FA/SDS/PMSF buffer (4 ml per extract). Spin down 1 min/2000 rpm. Gently resuspend the pellet with a 5 ml pipet and transfer in a sterile 15 ml Corex tube. Spin down 20 min / 12000 rpm / 4°C in a JA17 rotor (Beckmann). Vacuum the supernatant. The cross-linked chromatin appears as a transparent layer around the pellet. Transfer the pellet in a 2 ml Epp with a burned Pasteur pipet. Add 0.8 ml of FA/SDS/PMSF buffer in the Corex and resuspend with the Pasteur pipet. Transfer in the Epp. Wash the Corex with 0.8 ml FA/SDS/PMSF buffer. Homogenize the Epp with the Pasteur pipet and incubate on a rotating wheal at 4°C for 1 to 2 hours.Spin down 20 min / 12000 rpm / 4°C. Vacuum the supernatant and resuspend in 1.6 ml FA/SDS/PMSF buffer with a burned Pasteur pipet. Sonication step was performed on a S220 focused-ultrasonicator (Covaris) in 1mL milliTube (Covaris), for two cycles of 3 minutes spaced by a 30s rest time. Each cycle consisted of 150W pulses for 10% of the time (duty factor 10). Sonicated lysates were then transferred to a new 2mL safe-lock tube and centrifugation was performed at 15000g for 20 minutes. Average size of fragments obtained is 200bp.Transfer in a 12 ml Greiner tube and add 0.5 ml FA/SDS/PMSF buffer. Incubate on a rotating wheal for 30 min to 1 hour at 4°C. Spin down 30 min / 10000 rpm / 4°C on a JA20 rotor (Beckmann).Transfer the supernatant in a new tube. Aliquot the supernatant (the chromatin is now soluble). Aliquots of 650 µl and freeze in liquid nitrogen.Chromatin fragment control: Take an aliquot of 100 µl of chromatin and add 25 µl Pronase buffer 5X and 6.25 µl of Pronase (20 mg/ml in H20). Incubate 1 hour at 37 °C and O/N at 65 °C (digestproteins and reverse crosslink). Add 3.5 µl of RNaseA 1mg/ml and incubate 1 hour at 37 °C. Purify on a Quiagen PCR purification column (Elute wih 50 µl Tris 10 mM pH8.5). Load on gel (1.5% in TAE -100V).Immunoprecipitation:Wash 50 µl of Dynabeads Ig 4 times with 500 µl PBS BSA 0.1% buffer. Resuspend in 100 µl PBS BSA 0.1 % buffer. Add antibody. Incubate 30 min at 1300 rpm in an Eppendorf shaker at 30 °C. Wash 2X 500 µl PBS BSA 0.1%. Wash 1X 500 µl PBS BSA 0.1% with shaking 10 min at 1300 rpm / 30 °C. Wash 1X 500 µl PBS BSA 0.1%.Spin down the chromatin from the -80°C for 15 min / 12000 rpm / 4 °C. Add 50 µl of PBS BSA 10mg/ml to the beads and then 500 µl of chromatin.Incubate 2h at 21 °C in an Eppendorf shaker (1300 rpm). Resuspend with 500 µl FA/SDS and change tube. Wash 2X 1 ml FA/SDS + NaCl (final concentration 500 mM). Wash 1X 1 ml FA/SDS + NaCl with shaking 10 min at 1300 rpm / 21 °C. Wash 1X with 500 µl IP buffer (Tris pH7.5 125 mM, EDTA 25 mM, SDS 2.5%). Wash 1X with 500 µl TE. Elute with 125 µl Pronase Buffer 1X for 20 min at 65 °C in an Eppendorf shaker at 600 rpm. Cool down at RT. Add 6.25 µl Pronase to the supernatant. Incubate 1h at 37 °C and O/N at 65 °C. Add 25 µl of Pronase buffer 5X and 6.25 µl of Pronase to 100 µl of chromatin remaining from the initial tube (total DNA).Incubate 1h at 37 °C and O/N at 65 °C. Add 3.5 µl RNAseA (1mg/ml) and incubate 1h at 37 °C. Following a 75 minutes shift at 37°C, after reaching exponential phase at 25°C in YPD, cells were cross-linked with 1% formaldehyde during 10 minutes. DNA was purified on a Qiagen PCR purification column Libraries were constructed with TruSeq ChIP library preparation following manufacturer recommandations.

text: MATalpha ade2-1 lys2-801 ura3-52 trp1-D63 his3-D200 leu2D HIS3::3HA::RAD2 MED17::13Myc::TRP1 kin28::KanMX6 kin28ts16 LEU2 CEN

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Rad2-HA Med17-Myc kin28ts16

characteristic: strain


text: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris Sud, University Paris Saclay, F-91198 Gif-sur-Yvette cedex, France;

text: public

text: ArrayExpress

characteristic: description

text: MATalpha ade2-1 lys2-801 ura3-52 trp1-D63 his3-D200 leu2D HIS3::3HA::RAD2 MED17::13Myc::TRP1 kin28::KanMX6 KIN28 LEU2 CEN

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Rad2-HA Med17-Myc KIN28 WT


characteristic: INSDC center name

text: public

text: ArrayExpress

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Rad2-HA Med17-Myc kin28ts16


characteristic: INSDC last update

text: public

text: ArrayExpress

text: Protocols: The cell pellets are resuspended in 1 ml of FA/SDS/PMSF buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF) and transferred to 1.5 mL Eppendorf tubes. 0.75 mL glass beads (425-600 µm, Sigma) are then added to each tube, and the yeast cells were lysed at 4°C for 15 min by vortexing. Pierce the bottom of a 12 ml Greiner tube with a hot needle (0.7x30 mm or 0.5x15 mm) and place in a Falcon50 with a pierced cap. The lysates are transfered in the pierced tubes. Wash each Epp with twice 1 ml of FA/SDS/PMSF buffer (4 ml per extract). Spin down 1 min/2000 rpm. Gently resuspend the pellet with a 5 ml pipet and transfer in a sterile 15 ml Corex tube. Spin down 20 min / 12000 rpm / 4°C in a JA17 rotor (Beckmann). Vacuum the supernatant. The cross-linked chromatin appears as a transparent layer around the pellet. Transfer the pellet in a 2 ml Epp with a burned Pasteur pipet. Add 0.8 ml of FA/SDS/PMSF buffer in the Corex and resuspend with the Pasteur pipet. Transfer in the Epp. Wash the Corex with 0.8 ml FA/SDS/PMSF buffer. Homogenize the Epp with the Pasteur pipet and incubate on a rotating wheal at 4°C for 1 to 2 hours.Spin down 20 min / 12000 rpm / 4°C. Vacuum the supernatant and resuspend in 1.6 ml FA/SDS/PMSF buffer with a burned Pasteur pipet. Sonication step was performed on a S220 focused-ultrasonicator (Covaris) in 1mL milliTube (Covaris), for two cycles of 3 minutes spaced by a 30s rest time. Each cycle consisted of 150W pulses for 10% of the time (duty factor 10). Sonicated lysates were then transferred to a new 2mL safe-lock tube and centrifugation was performed at 15000g for 20 minutes. Supernatant was collected and combined with 300µL FA/SDS/PMSF. Sonicated chromatin was divided in 250µL aliquots, snap-frozen in liquid nitrogen and kept at -80°C until further use. Immunoprecipitation, DNA precipitation and library preparation were done on an IP-Star compact automated system (Diagenode) using built-in programs and following manufacturer’s instructions, with the following exceptions (Denby Wilkes et al., in preparation). IP was done using the ChIP_IPure_200_D program, at 22°C, with 1h “Ab coating” (Slow speed), 3h “IP reaction” (Medium speed) and 5 minutes “washes” (Fast speed). “Beads wash buffer” was PBS + 0.1% BSA, FA/SDS adjusted to 500mM NaCl was used for “IP Wash 1” and “IP Wash 2”, “IP Wash 3” was done with IP Buffer (Tris 10mM pH8, LiCl 0.25M, EDTA 1mM, NP40 0.5%, Na-Deoxycholate 0.5%), “IP Wash 4” was done with TE (Tris 10mM pH 8, EDTA 1mM). “Elution buffer” was the elution buffer (A+B) of Auto iPure kit V2 (Diagenode). “Ab coating mix” was 1µL anti-HA (12CA5), 2µL anti-Myc (9E10) or 5µL anti-Rpb1-CTD (8WG16) completed to 100µL with “Beads wash buffer”. “Sample” was 200µL sheared chromatin, supplemented with 4µL BSA and 4µL 50X Protease Inhibitor Cocktail (prepared by dissolving one cOmplete tablet in 1mL ddH2O). 20µL DiaMag protein A-coated magnetic beads (Diagenode) were used per sample. After program completion, strips containing the eluates were warmed to redissolve precipitated SDS, eluates were recovered using a magnetic stand, 5µL of 5M NaCl was added to eluates and they were incubated for 4h at 65°C to reverse crosslink. 1µL RNase A (ThermoFisher) was added to eluates and they were then incubated for 30 minutes at 37°C. After reaching exponential phase at 30°C in YPD, cells were cross-linked with 1% formaldehyde during 10 minutes. DNA was extracted using the Auto iPure V2 kit from Diagenode, following the manufacturer recommendations. Library construction was done according to the MicroPlex Library Preparation Kit v2 (Diagenode) protocole, following manufacturer's recommandation. Libraries were sized with AMPure beads (Beckman-coulter) to a final size of 400bp.

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 HIS3::3HA::RAD2 MED5::13Myc::KAN Dmed17::KanMX6::ADE2 / med17-Q444P CEN TRP1

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: med17/med17-Q444P TRP Med5-myc HA-Rad2


text: public

characteristic: INSDC status

text: ArrayExpress

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 HIS3::3HA::RAD2 MED5::13Myc::KAN Dmed17::KanMX6::ADE2 / med17-140 CEN TRP1

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: med17/med17-Q444P/M442L TRP Med5-myc HA-Rad2


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 HIS3::3HA::RAD2 MED5::13Myc::KAN Dmed17::kan::ADE2 / MED17 CEN TRP1

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: med17/MED17 TRP Med5-myc HA-Rad2


text: public

characteristic: SRA accession

text: ArrayExpress

text: MATalpha ade2-1 lys2-801 ura3-52 trp1-D63 his3-D200 leu2D HIS3::3HA::RAD2 MED17::13Myc::TRP1 RPB9::KanMX6

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: rpb9 Med17-myc HA-Rad2


text: public

text: ArrayExpress

text: MATalpha ade2-1 lys2-801 ura3-52 trp1-D63 his3-D200 leu2D HIS3::3HA::RAD2 MED17::13Myc::TRP1

characteristic: genotype

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Med17-myc HA-Rad2


E-MTAB-7081:M17_Myc Rpb9

text: public

text: E-MTAB-7081:M17_Myc Rpb9

text: ArrayExpress

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: rpb9 Med17-myc HA-Rad2

text: M17_Myc Rpb9


text: public

text: ArrayExpress

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 Dmed17::kan::ADE2 MED5::13myc::KANMX6 MED17 CEN URA3

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: med17/MED17 URA Med5-myc


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


text: Mycobacterium bovis

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Mycobacterium bovis

text: Mycobacterium bovis

text: Mycobacterium bovis

characteristic: title


Sardine Genome Assembly

characteristic: External Id

text: CCMAR

characteristic: INSDC first public

text: public

text: Sardine Genome Assembly

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

characteristic: organism

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Sardine Genome Assembly


Sardine Genome Raw Data

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Sardine Genome Raw Data

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Sardine Genome Raw Data

characteristic: title


E-MTAB-7079:MM98 miR-empty 1

text: public

text: E-MTAB-7079:MM98 miR-empty 1

tag: Namespace:INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy IGM - CNR, Pavia, Italy

text: ArrayExpress

characteristic: broker name

text: MES-MM 98

characteristic: cell line

text: mesothelial cell

characteristic: cell type

text: human

text: Protocols: Stable modulation of miR-24 expression by Lentiviral transduction. REN cell line reference paper : Smythe WR, Kaiser LR, Hwang HC, Amin KM, Pilewski JM, Eck SJ, et al. Successful adenovirus-mediated gene transfer in an in vivo model of human malignant mesothelioma. The Annals of thoracic surgery 1994;57:1395-401. MM98 cell line reference paper: Orecchia S, Schillaci F, Salvio M, Libener R, Betta PG. Aberrant E-cadherin and gamma-catenin expression in malignant mesothelioma and its diagnostic and biological relevance. Lung Cancer 2004;45 Suppl 1:S37-43 RNA extraction by Trizol reagent Random primed cDNA libraries were constructed from 100 ng of total RNA. A paired-end (2×125) run was performed on a Genome Sequencer Illumina HiSeq 2500 (GATC Biotech, Germany) with 50 million reads per sample.

characteristic: description

text: malignant pleural mesothelioma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mesothelium of pleural cavity

characteristic: organism part

text: pleural effusion

characteristic: sampling site

text: Homo sapiens

text: MM98 miR-empty 1


E-MTAB-7079:MM98 miR-empty 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy IGM - CNR, Pavia, Italy

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-7079:MM98 miR-empty 2

text: ArrayExpress

text: MES-MM 98

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: MM98 miR-empty 2


E-MTAB-7079:MM98 miR-empty 3

text: public

characteristic: SRA accession

text: E-MTAB-7079:MM98 miR-empty 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: MES-MM 98

text: mesothelial cell

text: human

characteristic: common name

text: malignant pleural mesothelioma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: MM98 miR-empty 3

characteristic: title


E-MTAB-7079:REN miR-empty 1

text: public

text: E-MTAB-7079:REN miR-empty 1

text: ArrayExpress

text: REN

characteristic: cell line

text: mesothelial cell

characteristic: cell type

text: human

characteristic: description

text: malignant pleural mesothelioma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: REN miR-empty 1


E-MTAB-7079:REN miR-empty 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-7079:REN miR-empty 2

text: ArrayExpress

text: REN

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mesothelium of pleural cavity

characteristic: organism part

text: pleural effusion

characteristic: sampling site

text: Homo sapiens

text: REN miR-empty 2


E-MTAB-7079:REN miR-empty 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-7079:REN miR-empty 3

text: ArrayExpress

text: REN

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: REN miR-empty 3


E-MTAB-7079:MM98 miR-Zip24 1

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7079:MM98 miR-Zip24 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: MES-MM 98

text: mesothelial cell

text: human

characteristic: common name

text: malignant pleural mesothelioma

text: miR-24-3p lentiviral knockdown

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: MM98 miR-Zip24 1


E-MTAB-7079:MM98 miR-Zip24 2

text: public

text: E-MTAB-7079:MM98 miR-Zip24 2

text: ArrayExpress

text: MES-MM 98

characteristic: cell line

text: mesothelial cell

characteristic: cell type

text: human

characteristic: description

text: malignant pleural mesothelioma

characteristic: disease

text: miR-24-3p lentiviral knockdown

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: MM98 miR-Zip24 2

characteristic: title


E-MTAB-7079:MM98 miR-Zip24 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-7079:MM98 miR-Zip24 3

text: ArrayExpress

text: MES-MM 98

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: miR-24-3p lentiviral knockdown

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mesothelium of pleural cavity

characteristic: organism part

text: pleural effusion

text: Homo sapiens

text: MM98 miR-Zip24 3


E-MTAB-7079:REN miR-Zip24 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-7079:REN miR-Zip24 1

text: ArrayExpress

text: REN

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: miR-24-3p lentiviral knockdown

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

characteristic: sampling site

text: Homo sapiens

text: REN miR-Zip24 1


E-MTAB-7079:REN miR-Zip24 2

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7079:REN miR-Zip24 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: REN

text: mesothelial cell

text: human

text: malignant pleural mesothelioma

text: miR-24-3p lentiviral knockdown

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: REN miR-Zip24 2


E-MTAB-7079:REN miR-Zip24 3

text: public

text: E-MTAB-7079:REN miR-Zip24 3

text: ArrayExpress

text: REN

characteristic: cell line

text: mesothelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: malignant pleural mesothelioma

characteristic: disease

text: miR-24-3p lentiviral knockdown

text: Homo sapiens

text: Homo sapiens

text: mesothelium of pleural cavity

text: pleural effusion

text: Homo sapiens

text: REN miR-Zip24 3


text: Homo sapiens

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: subject id

text: FD_seru_1 _Ctrl_6

characteristic: title


text: Homo sapiens

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _L_IL10_6


text: Homo sapiens

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IFNB_6


text: Homo sapiens

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IL4_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _P3C_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IFNG_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _MBP_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _sLPS_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _PIC_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _R848_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _CIL_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _Prec_D0


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _Ctrl_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IL10_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IFNB_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IL4_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _P3C_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _IFNG_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _sLPS_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _PIC_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _R848_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _MBP_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _CIL_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_seru_1 _Prec_D2


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _Ctrl_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _L_IL10_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IFNB_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IL4_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _P3C_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IFNG_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _sLPS_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _PIC_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _R848_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _MBP_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _CIL_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _Prec_D0


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _Ctrl_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IL10_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IFNB_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IL4_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _P3C_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _IFNG_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _sLPS_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _PIC_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _R848_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _MBP_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _CIL_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tolg_4 _Prec_D2


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _Ctrl_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _L_IL10_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IFNB_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IL4_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _P3C_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IFNG_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _sLPS_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _PIC_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _R848_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _MBP_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _CIL_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _Prec_D0


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _Ctrl_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IL10_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IFNB_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IL4_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _P3C_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _IFNG_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _sLPS_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _PIC_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _R848_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _MBP_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _CIL_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_aowh_2 _Prec_D2


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _Ctrl_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _L_IL10_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IFNB_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IL4_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _P3C_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IFNG_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _sLPS_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _PIC_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _R848_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _MBP_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _CIL_6


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _Prec_D0


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _Ctrl_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IL10_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IFNB_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IL4_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _P3C_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _IFNG_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _sLPS_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _PIC_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _R848_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _MBP_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _CIL_24


text: Homo sapiens

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: FD_tert_1 _Prec_D2


text: Klebsiella pneumoniae

text: SC

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: The Francis Crick Institute

text: public

tag: Namespace:The Francis Crick Institute

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: HepG2 cells were cultured in Minimum Essential Media (MEM) with 10% FBS. All media was supplemented with 2mM L-glutamine, 100U/ml penicillin, and 100μg/ml streptomycin. HepG2 cells inducible for Cas9 expression were generated as previously described (Henser-Brownhill et al., 2017). HepG2 cells were treated with the HDAC inhibitor Trichostatin A, which induces histone hyperacetylation, and the EZH2 inhibitor GSK126, which globally reduces H3K27Me3 levels. Cells pre-treated with TSA (11nM or 100nM) or EZH2 (0.3 µM and 3 µM) for 5 days were infected with sgRNAs targeting the ACTL6A.5, ASF1B.7, BRD2.7, MBD3L1.6, MSH6.2 and SMARCD2.1 sites. Cas9 induction was induced with 1ug/mL doxycycline 1 day prior to infection with the sgRNAs and sustained until cells were harvested for genomic DNA. Genomic DNA was extracted 5 days after infection (Qiagen 69506). For the first PCR, 150ng of the corresponding gDNA were amplified for 20-22 cycles using the Herculase II polymerase kit yielding products of ~500bp. Next, PCR products were purified as per manufacturer’s instructions (Qiagen 28106) and 1µl of the resulting product was used as a template for the second nested PCR reaction in which primers containing barcodes and adapters for the sequencing reaction were added.

characteristic: description

text: hepatocellular carcinoma

text: CRISPR/Cas9 mediated double knockout of ACTL6A.5 and MBD3L1.6 sites

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: hepatocellular carcinoma

characteristic: disease

text: CRISPR/Cas9 mediated double knockout of ASF1B.7 and MSH6.2 sites

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

text: public

text: ArrayExpress

text: epithelial cell

text: human

text: hepatocellular carcinoma

text: CRISPR/Cas9 mediated double knockout of BRD2.7 and SMARCD2.1 sites

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

characteristic: title


text: CCMAR

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: White Muscle

characteristic: tissue

text: Sardine Raw Reads Wm


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Brain + Pituitary gland

text: Sardine Raw Reads Br

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Caudal Fin (Skin + Cartilage + Bone)

characteristic: tissue

text: Sardine Raw Reads Cf


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Gill + Branchial Arch

text: Sardine Raw Reads Gi

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Gonad

characteristic: tissue

text: Sardine Raw Reads Gn


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Head Kidney

text: Sardine Raw Reads Hk

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Kidney

characteristic: tissue

text: Sardine Raw Reads Kd


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Liver

text: Sardine Raw Reads Lv

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Midgut

characteristic: tissue

text: Sardine Raw Reads Mg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Red Muscle

text: Sardine Raw Reads Rm

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Spleen

characteristic: tissue

text: Sardine Raw Reads Sp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Brain + Pituitary gland

text: Sardine Transcriptome Br

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Caudal Fin (Skin + Cartilage + Bone)

characteristic: tissue

text: Sardine Transcriptome Cf


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Gill + Branchial Arch

text: Sardine Transcriptome Gi

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Gonad

characteristic: tissue

text: Sardine Transcriptome Gn


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Head Kidney

text: Sardine Transcriptome Hk

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Kidney

characteristic: tissue

text: Sardine Transcriptome Kd


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Liver

text: Sardine Transcriptome Lv

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Midgut

characteristic: tissue

text: Sardine Transcriptome Mg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: Red Muscle

text: Sardine Transcriptome Rm

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Spleen

characteristic: tissue

text: Sardine Transcriptome Sp


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCMAR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: kept in captivity for 17 months

characteristic: animal care environment

text: EPPO/IPMA, Olhão

characteristic: aquaculture origin

text: sardine

characteristic: common name

text: adult

text: Coastal Atlantic Ocean

text: Coastal Atlantic Ocean

text: water

text: fish feed

text: Portugal

text: Algarve

text: 36.962889 N 7.816861 W

text: Coastal Atlantic Ocean

text: Sardina pilchardus

characteristic: organism

text: fisheries net

text: Sardina pilchardus

text: female

text: visual assesment

text: White Muscle

text: Sardine Transcriptome Wm

characteristic: title


text: CCMAR

text: public

text: kept in captivity for 17 months

text: EPPO/IPMA, Olhão

characteristic: body length

characteristic: collection date

text: sardine

characteristic: depth

text: adult

characteristic: developmental stage

text: Coastal Atlantic Ocean

characteristic: environment biome

text: Coastal Atlantic Ocean

characteristic: environment feature

text: water

characteristic: environment material

text: fish feed

characteristic: feeding nature

text: Portugal

characteristic: geographic location (country and/or sea)

text: Algarve

characteristic: geographic location (region and locality)

text: 36.962889 N 7.816861 W

characteristic: lat lon

text: Coastal Atlantic Ocean

characteristic: marine region

text: Sardina pilchardus

text: fisheries net

characteristic: sample collection device

text: Sardina pilchardus

text: female

characteristic: sex

text: visual assesment

characteristic: taxonomy determination methods

text: Sardine Transcriptome


text: Mycobacterium abscessus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Mycobacterium abscessus

characteristic: common name

text: Mycobacteroides abscessus

characteristic: organism

text: Mycobacteroides abscessus

characteristic: subject id

text: Mycobacterium abscessus


CECT 7235

text: UVEG

text: public

text: CECT 7235

characteristic: collection date

text: Water

characteristic: environment (biome)

text: Sample taken at 6 m depth at the hypersaline, meromictic and heliothermal Ekho

characteristic: environment (feature)

characteristic: environment (material)

text: Antarctica

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Roseibaca ekhonensis

text: Whole genome sequencing of Roseibaca ekhonensis CECT 7235, type strain

characteristic: project name

text: Roseibaca ekhonensis

text: Illumina Miseq

characteristic: sequencing method

text: CECT 7235

characteristic: strain

text: Roseibaca ekhonensis CECT 7235

characteristic: title

text: water

characteristic: water environmental package


name: ChIRP-seq of Plasmodium falciparum_Input

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tongji University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Tongji University School of Medicine

characteristic: Submitter Id

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: ChIRP-seq reveals the GC-rich ncRNA is required for monoallelic activation of virulence genes in Plasmodium falciparum


ChIRP-seq of Plasmodium falciparum_1

text: Tongji University School of Medicine

text: public

text: ChIRP-seq of Plasmodium falciparum_1

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum


ChIRP-seq of Plasmodium falciparum_2

text: Tongji University School of Medicine

text: public

text: ChIRP-seq of Plasmodium falciparum_2

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: CNGB

text: public

text: CNSA

characteristic: broker name

characteristic: collection date

characteristic: description

text: Netherlands : Maastricht

characteristic: geographic location

text: human

characteristic: host

text: 50.85 N 5.69 E

characteristic: latitude and longitude

text: human gut metagenome

characteristic: sample name

text: human gut metagenome

text: human gut metagenome


text: Schistosoma mansoni

characteristic: ArrayExpress-Species

text: NMRI

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Schistosoma mansoni

text: Schistosoma mansoni

text: Schistosoma mansoni

text: NMRI

characteristic: strain

text: Schistosoma mansoni

characteristic: title


characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF MANCHESTER

characteristic: Submitter Id

text: ArrayExpress

text: female germline stem cell

characteristic: cell type

text: fruit fly

text: Protocols: Ovaries are dissected into 1x PBS on ice and incubated in 5 mg/ml collagenase IV in PBS (Worthington Biochemicals) at RT for 45 min to dissociate the tissue. Cells were washed in PBS and filtered through a 40 μm nylon mesh to remove debris before fluorescence-activated cell sorting (FACS) based on the expression of the germline marker vasa.GFP or differentiation factor bam.GFP using a FACSAria™ Fusion cell sorter (Diva 8 Software; BD Biosciences). Cells were sorted into 1x PBS on ice and RNA was isolated. RNA was isolated using TRIzol (Invitrogen). Total RNA was processed and sequenced. TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer’s protocol

text: adult

characteristic: developmental stage

text: vasaGFP; nos-Gal4; UASp-bamshRNA

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

characteristic: organism part

text: bam knockdown by shRNA

characteristic: phenotype

text: Drosophila melanogaster

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MANCHESTER

text: public

text: ArrayExpress

characteristic: broker name

text: cystoblast

text: fruit fly

characteristic: common name

characteristic: description

text: adult

text: bam-GFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: ovary

text: bam-GFP

text: Drosophila melanogaster

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF MANCHESTER

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: female germline stem cell

text: fruit fly

text: adult

text: vasaGFP; nos-Gal4; UASp-tkvQ253D

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

text: high BMP expression; constitutively active tkv expression

text: Drosophila melanogaster

text: female

characteristic: title


text: UNIVERSITY OF OVIEDO

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF OVIEDO

characteristic: Submitter Id

text: house mouse

text: Heart biopsy miR-29a/b1 KO mouse 1

text: adult

characteristic: dev stage

text: Mus musculus

text: Mus musculus

text: heart

characteristic: tissue lib


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy miR-29a/b1 KO mouse 2

text: adult

text: Mus musculus

text: Mus musculus

text: heart


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy miR-29a/b1 KO mouse 3

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy miR-29c/b2 KO mouse 1

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy miR-29c/b2 KO mouse 2

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy miR-29c/b2 KO mouse 3

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy wildtype mouse 1

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy wildtype mouse 2

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: UNIVERSITY OF OVIEDO

text: public

text: house mouse

text: Heart biopsy wildtype mouse 3

text: adult

text: Mus musculus

text: Mus musculus

text: heart


text: public

tag: Namespace:TECHNICAL UNIVERSITY OF DENMARK, NATIONAL VETERINARY INSTITUTE

characteristic: collection date

text: Denmark

characteristic: geographic location (country and/or sea)

text: Myotis daubentonii

characteristic: host scientific name

text: Bat coronavirus

text: Bat coronavirus

text: Alphacoronavirus from Myotis daubentonii in Denmark


text: TECHNICAL UNIVERSITY OF DENMARK, NATIONAL VETERINARY INSTITUTE

text: public

text: Denmark

text: Myotis daubentonii

text: Bat coronavirus

characteristic: organism

text: Bat coronavirus


characteristic: ENA-LAST-UPDATE

text: public

text: Myotis daubentonii

text: Bat coronavirus

text: Bat coronavirus


characteristic: External Id

text: STOCKHOLM UNIVERSITY

text: public

tag: Namespace:STOCKHOLM UNIVERSITY

text: specked wood butterfly

characteristic: common name

text: Pararge aegeria

text: Pararge aegeria

text: Genome assembly


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Myotis dasycneme

text: Bat coronavirus

text: Bat coronavirus

text: Alphacoronavirus from Myotis dasycneme in Denmark


characteristic: INSDC last update

text: public

text: Denmark

text: Pipistrellus pygmaeus

text: Bat coronavirus

text: Bat coronavirus

text: Alphacoronavirus from Pipistrellus pygmaeus in Denmark

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

text: Denmark

text: Eptesicus serotinus

text: Bat coronavirus

text: Bat coronavirus

text: Alphacoronavirus from Eptesicus serotinus in Denmark


text: public

characteristic: Submitter Id

text: Denmark

text: Myotis nattereri

text: Bat coronavirus

text: Bat coronavirus

text: Alphacoronavirus from Myotis nattereri in Denmark


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: subject id


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: HepG2 cells were cultured in Minimum Essential Media (MEM) with 10% FBS. All media was supplemented with 2mM L-glutamine, 100U/ml penicillin, and 100μg/ml streptomycin. sgRNA pools are described in the additional files associated with the submission Table_S1_sgrna_metadata.tsv and Table_S6_pools.tsv. HepG2 cells inducible for Cas9 expression were generated as previously described (Henser-Brownhill et al., 2017; 10.1080/15592294.2017.1395121). Cas9 induction was induced with 1ug/mL doxycycline 1 day prior to infection with the sgRNAs and sustained until cells were harvested for genomic DNA extraction. Transduced cells were selected with 4ug/mL blasticidin, starting 24h after the first infection, and genomic DNA was extracted 5 days after infection (Qiagen 69506). DNA libraries enriched for the targeted sites were generated using the SureSelect Target enrichment kit (Agilent) following the manufacturer’s instructions. Capture probes were designed to cover 2Kb regions centered on each target site.

characteristic: description

text: hepatocellular carcinoma

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

text: hepatocellular carcinoma

text: CRISPR/Cas9 mediated knockout with sgRNA pool 450-5

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens


text: The Francis Crick Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: hepatocellular carcinoma

text: CRISPR/Cas9 mediated knockout with sgRNA pool 450-6

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

text: human

text: hepatocellular carcinoma

text: CRISPR/Cas9 mediated knockout with sgRNA pool 450-7

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens