characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Subsoil (20-40 cm) from position x = -114 m along the transect

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.391951° N

text: 23.112378° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

tag: Namespace:ETH ZURICH

characteristic: Submitter Id

characteristic: collection date

text: Subsoil (20-40 cm) from position x = -139 m along the transect

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

unit: m

text: 43.391727° N

unit: DD

characteristic: geographic location (latitude)

text: 23.11234° E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

text: Prokaryotic communities in Ogosta floodplain soils

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Subsoil (20-40 cm) from position x = -189 m along the transect

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.391281° N

text: 23.112264° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Subsoil (20-40 cm) from position x = -214 m along the transect

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

characteristic: geographic location (latitude)

text: 23.112226° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Subsoil (20-40 cm) from position x = -239 m along the transect

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.390835° N

text: 23.112188° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Subsoil (20-40 cm) from position x = -289 m along the transect

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.390389° N

text: 23.112112° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Subsoil (20-40 cm) from position x = -314 m along the transect

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.390166° N

characteristic: geographic location (latitude)

text: 23.112073° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Subsoil (20-40 cm) from position x = -339 m along the transect

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.389942° N

text: 23.112035° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Subsoil (20-40 cm) from position x = -389 m along the transect

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.389496° N

text: 23.111959° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Subsoil (20-40 cm) from position x = -439 m along the transect

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.38905° N

characteristic: geographic location (latitude)

text: 23.111883° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P1 from 0-5 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

text: 23.112367° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P1 from 5-10 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.39255° N

text: 23.112367° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P1 from 10-15 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P1 from 15-20 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

text: 23.112367° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P1 from 20-30 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.39255° N

text: 23.112367° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P1 from 30-40 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

characteristic: geographic location (latitude)

text: 23.112367° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P1 from 40-46 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

text: 23.112367° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P1 from 46-50 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.39255° N

text: 23.112367° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P1 from 50-60 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

characteristic: geographic location (latitude)

text: 23.112367° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: Prokaryotic communities in Ogosta floodplain soils

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P1 from 60-70 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

text: 23.112367° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P1 from 70-80 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.39255° N

text: 23.112367° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P1 from 80-90 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.39255° N

characteristic: geographic location (latitude)

text: 23.112367° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P3 from 0-5 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

text: 23.112226° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P3 from 5-10 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.391058° N

text: 23.112226° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P3 from 10-15 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

characteristic: geographic location (latitude)

text: 23.112226° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P3 from 15-20 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

text: 23.112226° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P3 from 20-30 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.391058° N

text: 23.112226° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Soil from soil profile P3 from 30-40 cm depth

characteristic: description

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

characteristic: geographic location (latitude)

text: 23.112226° E

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: ETH ZURICH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Soil from soil profile P3 from 40-50 cm depth

text: grassland

text: floodplain

text: soil

text: Bulgaria

text: 43.391058° N

text: 23.112226° E

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Ogosta floodplain soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH

characteristic: INSDC center name

text: public

text: Soil from soil profile P3 from 50-60 cm depth

text: grassland

characteristic: environment (biome)

text: floodplain

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Bulgaria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 43.391058° N

text: 23.112226° E

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Ogosta floodplain soil


characteristic: ENA-FIRST-PUBLIC

text: UNIV-WUE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Heriades truncorum

characteristic: host scientific name

text: Paenibacillus polymyxa

text: Paenibacillus polymyxa


text: soil metagenome

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

text: soil metagenome

characteristic: common name

text: soil metagenome

characteristic: organism

text: soil metagenome

characteristic: subject id

characteristic: title


E-MTAB-6889:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6889:Sample 1

tag: Namespace:Laboratory of Meolcularc Medicine and Genomics

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: mammary epithelial cell

characteristic: cell type

text: human

text: Protocols: ER-alpha positive BC cells MCF-7 (ATCC HTB-22) were propagated in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich) supplemented with 10% FBS (HyClone) and antibiotics: 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml amphotericin-B For histone extraction 100.000 cells/well were seeded in 35 mm plates were incubated with increasing concentrations of Dot1L inhibitors: EPZ004777 (S7353, Sellekchem), EPZ5676 (S7062 Sellekchem) and SGC0946 (S7079, Sellekchem) and their vehicle for 6 days. For this experiment we use only EPZ004777 (S7353, Sellekchem) at 6.4 microM. Cells were harvested, washed twice with ice-cold PBS and the pellets were resuspended in Triton Extraction Buffer (TEB: PBS containing 0.5% Triton X 100 (v/v), 2 mM phenylmethylsulfonyl fluoride (PMSF). Cells were lysed on ice for 10 min and nuclear separation was obtained by centrifugation at 6,500 x g for 10 min at 4°C. Then, the nuclei were resuspended in 0.2 N HCl before acid extraction of histones over night at 4°C. The histone content was determined using the Bradford assay and was analyzed by Western blot procedure. Antibody used:: Rb pAb Histone3 (di methyl k79); catalogue no: ab3594; Company: abcam After binding, beads washing, elution, reverse crosslinking and DNA extraction were performed as described in Tarallo et al, 2014. Three biological replicates were performed for each experimental condition. Then, 1µl aliquot of obtained DNA was used to asses size distribution by Agilent Genomic DNA ScreenTape & Reagents using an Agilent 4200 TapeStation System (Agilent Technologies). The concentration of each DNA sample was determined by using Quant-IT DNA Assay Kit-High Sensitivity and a Qubit Fluorometer (Life Technologies). 10 ng of each purified ChIP DNA were used for sequencing libraries preparation with TruSeq ChIP Sample Prep Kit (Illumina Inc.).

characteristic: description

text: invasive ductal carcinoma

characteristic: disease

text: pleural effusion

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-6889:Sample 2

characteristic: ENA-FIRST-PUBLIC

text: Laboratory of Meolcularc Medicine and Genomics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6889:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: mammary epithelial cell

text: human

text: invasive ductal carcinoma

text: pleural effusion

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 2


E-MTAB-6889:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-6889:Sample 3

text: ArrayExpress

text: mammary epithelial cell

text: human

characteristic: common name

text: invasive ductal carcinoma

text: pleural effusion

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-6889:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-6889:Sample 4

text: ArrayExpress

characteristic: broker name

text: mammary epithelial cell

text: human

text: invasive ductal carcinoma

text: pleural effusion

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 4


E-MTAB-6889:Sample 5

text: public

text: E-MTAB-6889:Sample 5

text: ArrayExpress

characteristic: cell line

text: mammary epithelial cell

characteristic: cell type

text: human

characteristic: description

text: invasive ductal carcinoma

characteristic: disease

text: pleural effusion

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Sample 5


E-MTAB-6889:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

text: public

text: E-MTAB-6889:Sample 6

text: ArrayExpress

text: mammary epithelial cell

text: human

text: invasive ductal carcinoma

text: pleural effusion

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Sample 6


text: CIC BIOGUNE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:CIC BIOGUNE

characteristic: Submitter Id

characteristic: collection date

text: human

text: feces

characteristic: environment (biome)

text: feces

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: Illumina

characteristic: sequencing method

text: feces microbiome in fibromyalgia patients


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Costa Rica

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:University of Costa Rica

text: ArrayExpress

characteristic: broker name

text: Protocols: Mycelium was harvested and springtails were removed. Whole mycelium was used to extract the RNA. 5,000 fungal spores in 5 µL water were inoculated onto three rice kernels autoclaved with 200 µl demineralized water in 15 ml Falcon tubes and incubated at 15°C in dark for 7-8 days 20 mg (around 200 individual animal) of springtail Folsomia candida starved for two days were placed into mycelia and the incubated at 15°C in dark. Controls were incubated under the same conditions without animals. Each group consisted of 4 replicates. Suspension of mycelia in 400 µl of RNA extraction solution [95% deionized formamide, 18 mM EDTA, 0.025% SDS, and 1% 2-mercaptoethanol] was disrupted by shaking with zirconia beads (2.0 mm diameter, Carl Roth, Karlsruhe, Germany) in reciprocal mill MM 200 (Retsch, Haan, Germany) for 2 min at maximum power, followed by incubation at 95°C for 7 min. Cells debris was removed by centrifugation at 16,000x g for 5 min at room temperature. The supernatant was transferred into RNA precipitation mixture consisting of 800 µl of isobutanol, 400 µl of 5 M guanidine thiocyanate and 5 µl of linear polyacrylamide used as co-precipitant (Co-Precipitant Pink, Bioline, London, UK). The mixture was centrifuged at 16,000x g for 5 min at room temperature and the pellet was washed with 75% ethanol, dried and dissolved in RNAase-free water. Strand-specific cDNA libraries were prepared using Illumina’s TruSeq stranded mRNA kit (75 bp paired-end).

characteristic: description

text: without Folsomia candida

characteristic: growth condition

text: Fusarium graminearum

characteristic: organism

text: Fusarium graminearum

text: mycelium

characteristic: replicate

text: Fusarium graminearum

text: controlA

characteristic: title


text: University of Costa Rica

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: without Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

characteristic: organism part

text: Fusarium graminearum

text: controlB


characteristic: ENA-CHECKLIST

text: University of Costa Rica

text: public

text: ArrayExpress

text: without Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: controlC


characteristic: ENA-FIRST-PUBLIC

text: University of Costa Rica

text: public

text: ArrayExpress

text: without Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: controlD


characteristic: ENA-LAST-UPDATE

text: University of Costa Rica

text: public

text: ArrayExpress

text: with Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: TreatedA


characteristic: External Id

text: University of Costa Rica

text: public

text: ArrayExpress

text: with Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: TreatedB


text: University of Costa Rica

characteristic: INSDC center name

text: public

text: ArrayExpress

text: with Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: TreatedC


text: University of Costa Rica

characteristic: INSDC first public

text: public

text: ArrayExpress

text: with Folsomia candida

text: Fusarium graminearum

text: Fusarium graminearum

text: mycelium

text: Fusarium graminearum

text: TreatedD


text: UZH

text: public

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: reticular cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Ccl19-Cre mice were crossed with R26-stop-EYFP (Ccl19-EYFP). To specifically delete Myd88 from fibroblastic stromal cells (FRCs), Ccl19-EYFP mice were crossed with Myd88fl/fl mice (Ccl19-EYFP Myd88fl/fl ). Ccl19-EYFP and Ccl19-EYFP Myd88fl/fl mice were immunized or not with 10µg of Salmonella typhi OmpC/F porin intraperitoneally. Omentum was collected from naive or immunized mice at day 4 post-administration. CD45- CD31- Ter119- EYFP+ fibroblastic reticular cells from Ccl19-EYFP and Ccl19-EYFP Myd88fl/fl were sorted from independent mice with a Bio-Rad S3 cell sorter and were collected in 200 µl Eppendorf tubes containing 100 µl RNAlater reagent (Qiagen) to preserve the RNA after sorting. Note: BAC-transgenic C57BL/6N-Tg(Ccl19-cre)489Biat mice (Ccl19-Cre mice) Ccl19-Cre were crossed with Gt(ROSA)26Sortm1(EYFP)Cos (Ccl19-EYFP) Ccl19-EYFP were crossed with the Myd88tm1Defr (Ccl19-EYFP Myd88fl/fl) RNA extraction was performed with Quick-RNA Micro-Prep (Zymo Research). Reverse transcription and cDNA library generation was done using the Ovation SoLo RNA-Seq System (NuGEN) as described by the manufacturer. cDNA libraries were quantified by KAPA library Quantification kit (KAPA Biosystems) and Agilent Tape station in the Functional Genomics Center Zurich, Zurich, Switzerland.

text: normal

characteristic: disease

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: omentum

text: Ccl19 positive

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


text: UZH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: reticular cell

text: house mouse

characteristic: description

text: normal

text: Mus musculus

characteristic: organism

text: Mus musculus

text: omentum

text: Ccl19 positive

text: Mus musculus

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UZH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6886:20180219.A-Naive_MyD88_CKO_1

text: ArrayExpress

text: reticular cell

text: house mouse

text: normal

text: Ccl19-EYFP Myd88fl/fl

text: Mus musculus

text: Mus musculus

text: omentum

characteristic: organism part

text: Ccl19 positive

text: Mus musculus

text: female


text: UZH

text: public

text: E-MTAB-6886:20180219.A-Naive_MyD88_CKO_2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: reticular cell

characteristic: cell type

text: house mouse

characteristic: common name

text: normal

characteristic: disease

text: Ccl19-EYFP Myd88fl/fl

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: omentum

text: Ccl19 positive

characteristic: phenotype

text: Mus musculus

text: female


text: UZH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6886:20180219.A-Naive_MyD88_CKO_3

characteristic: Submitter Id

text: ArrayExpress

text: reticular cell

text: house mouse

characteristic: description

text: normal

text: Ccl19-EYFP Myd88fl/fl

text: Mus musculus

text: Mus musculus

text: omentum

text: Ccl19 positive

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UZH

characteristic: INSDC center name

text: public

text: E-MTAB-6886:20180219.A-Naive_MyD88_CKO_4

text: ArrayExpress

text: reticular cell

text: house mouse

text: normal

text: Ccl19-EYFP Myd88fl/fl

text: Mus musculus

characteristic: organism

text: Mus musculus

text: omentum

text: Ccl19 positive

text: Mus musculus

text: male

characteristic: title


text: UZH

characteristic: INSDC first public

text: public

text: E-MTAB-6886:20180219.A-Naive_MyD88_CKO_5

characteristic: age

text: ArrayExpress

characteristic: broker name

text: reticular cell

characteristic: cell type

text: house mouse

characteristic: common name

text: normal

characteristic: disease

text: Ccl19-EYFP Myd88fl/fl

text: Mus musculus

text: Mus musculus

text: omentum

characteristic: organism part

text: Ccl19 positive

text: Mus musculus

text: male


text: UZH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6886:20180219.A-Porin_Day_4_EYFP_1

characteristic: Submitter Id

text: ArrayExpress

text: reticular cell

text: house mouse

characteristic: description

text: normal

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: omentum

text: Ccl19 positive

characteristic: phenotype

text: Mus musculus

text: female

text: OmpC/F; 10 microgram

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UZH

characteristic: INSDC center name

text: public

text: E-MTAB-6886:20180219.A-Porin_Day_4_EYFP_4

text: ArrayExpress

text: reticular cell

text: house mouse

text: normal

text: Mus musculus

characteristic: organism

text: Mus musculus

text: omentum

text: Ccl19 positive

text: Mus musculus

text: male

characteristic: sex

text: OmpC/F; 10 microgram

characteristic: strain


text: UZH

characteristic: INSDC first public

text: public

text: E-MTAB-6886:20180219.A-Porin_Day_4_MyD88_CKO_1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: reticular cell

characteristic: cell type

text: house mouse

characteristic: common name

text: normal

characteristic: disease

text: Ccl19-EYFP Myd88fl/fl

text: Mus musculus

text: Mus musculus

text: omentum

characteristic: organism part

text: Ccl19 positive

text: Mus musculus

text: female

text: OmpC/F; 10 microgram

characteristic: title


text: UZH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6886:20180219.A-Porin_Day_4_MyD88_CKO_3

characteristic: Submitter Id

text: ArrayExpress

text: reticular cell

text: house mouse

characteristic: description

text: normal

text: Ccl19-EYFP Myd88fl/fl

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: omentum

text: Ccl19 positive

characteristic: phenotype

text: Mus musculus

text: male

text: OmpC/F; 10 microgram

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Max Planck Institute for the Science of Human History

text: human

text: Jordan

characteristic: geographic location (country and/or sea)

text: Baja

characteristic: geographic location (region and locality)

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: Max Planck Institute for the Science of Human History

characteristic: INSDC first public

text: public

text: human

characteristic: common name

text: Israel

text: Kfar HaHoresh

text: Homo sapiens

text: Homo sapiens

characteristic: title


ZBC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ZBC

text: human

text: Turkey

text: Pinarbasi

text: Homo sapiens

text: Homo sapiens


ZHAG

text: public

text: ZHAG

characteristic: Submitter Id

text: human

text: Turkey

text: Boncuklu

text: Homo sapiens

text: Homo sapiens


ZHAJ

text: public

text: ZHAJ

text: human

text: Turkey

text: Boncuklu

text: Homo sapiens

text: Homo sapiens


ZHJ

text: public

text: ZHJ

text: human

text: Turkey

text: Boncuklu

text: Homo sapiens

text: Homo sapiens


ZKO

text: public

text: ZKO

text: human

text: Turkey

text: Boncuklu

text: Homo sapiens

text: Homo sapiens


ZMOJ

text: public

text: ZMOJ

text: human

text: Turkey

text: Boncuklu

text: Homo sapiens

text: Homo sapiens


text: Medical University of Vienna

text: public

tag: Namespace:Medical University of Vienna

text: human

text: Homo sapiens

text: Homo sapiens

text: ViromeLuTx


CPBF 1521

text: public

text: CPBF 1521

tag: Namespace:Zurich University of Applied Sciences (ZHAW), School of Life Sciences and Facility Management, Institute of Natural Resource Sciences

characteristic: collection date

text: Xanthomonas arboricola pv. juglandis CPBF 1521 isolated from a walnut leaf (J. regia) presenting typical walnut bacterial blight symptoms

characteristic: description

text: Portugal

text: Loures

text: diseased

characteristic: host health state

text: Juglans regia

characteristic: host scientific name

text: CPBF 1521

characteristic: isolate

text: Walnut leaf (J. regia) presenting typical walnut bacterial blight symptoms

characteristic: isolation source

text: Xanthomonas arboricola pv. juglandis

text: Xanthomonas arboricola pv. juglandis

text: CPBF 1521

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

text: Whole Foods

characteristic: brand

text: mm

text: stilton

characteristic: cheese type

text: Stilton Whole Foods Market Borough Market

characteristic: date collected

text: 5 x 5 mm cube of cheese

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: blue cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Stilton

text: food metagenome

characteristic: ph

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: poh

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

text: Whole Foods

characteristic: brand

text: mm

text: stilton

characteristic: cheese type

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: blue cheese

text: USA:CA:San Diego

text: Stilton

text: food metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: cheese

text: food metagenome

text: TRUE

characteristic: title

text: cheese niche study


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

text: public

text: cheese

text: JM

text: no

text: Whole Foods

text: mm

text: bonde

text: Bonde Du Poitou Herve Mons

text: 5 x 5 mm cube of cheese

text: TRUE

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: bitter cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Bonde

text: food metagenome

characteristic: ph

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: poh

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

text: Whole Foods

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

text: Whole Foods

characteristic: brand

text: mm

text: bonde

characteristic: cheese type

text: Bonde Du Poitou Herve Mons

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: bitter cheese

text: USA:CA:San Diego

text: Bonde

text: food metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: cheese

text: food metagenome

text: TRUE

text: Whole Foods

text: cheese niche study

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

text: public

text: cheese

text: JM

text: no

text: Whole Foods

text: mm

text: bonde

text: Bonde Du Poitou Herve Mons

text: 5 x 5 mm cube of cheese

text: TRUE

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: bitter cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Bonde

text: food metagenome

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

text: Whole Foods

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

text: Whole Foods

characteristic: brand

text: mm

text: bonde

characteristic: cheese type

text: Bonde Du Poitou Herve Mons

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: bitter cheese

text: USA:CA:San Diego

text: Bonde

text: food metagenome

characteristic: organism

characteristic: ph

text: UCSDMI

text: TRUE

characteristic: poh

text: cheese

text: food metagenome

text: TRUE

text: Whole Foods

text: cheese niche study

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: cheese

text: JM

text: no

text: Whole Foods

text: mm

text: bonde

text: Bonde Du Poitou Herve Mons

text: 5 x 5 mm cube of cheese

text: TRUE

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: bitter cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Bonde

text: food metagenome

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

text: Whole Foods

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

text: Whole Foods

characteristic: brand

text: mm

text: bonde

characteristic: cheese type

text: Bonde Du Poitou Herve Mons

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: bitter cheese

text: USA:CA:San Diego

text: Bonde

text: food metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: cheese

text: food metagenome

text: TRUE

text: Whole Foods

characteristic: title

text: cheese niche study


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: cheese

text: JM

text: no

text: mm

text: stilton

text: 5 x 5 mm cube of cheese

text: TRUE

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: blue cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Stilton

text: food metagenome

characteristic: ph

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: poh

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

characteristic: brand

text: mm

text: stilton

characteristic: cheese type

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: blue cheese

text: USA:CA:San Diego

text: Stilton

text: food metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: cheese

text: food metagenome

text: TRUE

text: cheese niche study

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

text: public

text: cheese

text: JM

text: no

text: mm

text: stilton

text: 5 x 5 mm cube of cheese

text: TRUE

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: blue cheese

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: Stilton

text: food metagenome

characteristic: ph

text: UCSDMI

text: TRUE

characteristic: plate name

characteristic: poh

characteristic: radius

text: cheese

characteristic: sample type

text: food metagenome

text: TRUE

characteristic: source

text: cheese niche study

characteristic: well position

characteristic: x

characteristic: y

characteristic: z


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cheese

characteristic: alias

text: JM

characteristic: anonymized name

text: no

characteristic: brand

text: mm

text: stilton

characteristic: cheese type

characteristic: date collected

text: 5 x 5 mm cube of cheese

characteristic: description

text: TRUE

characteristic: elevation

text: Free-living

text: Non-saline

text: Surface (non-saline)

text: urban biome

characteristic: env biome

text: research facility

text: misc environment

text: blue cheese

text: USA:CA:San Diego

text: Stilton

text: food metagenome

characteristic: organism

text: UCSDMI

text: TRUE

text: cheese

text: food metagenome

text: TRUE

characteristic: title

text: cheese niche study


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

text: public

text: blank

text: TRUE

text: Control

text: Negative

text: Sterile water blank

text: urban biome

text: research facility

characteristic: env feature

text: misc environment

characteristic: env package

text: sterile water

text: USA:CA:San Diego

characteristic: geo loc name

characteristic: latitude

characteristic: longitude

text: metagenome

text: UCSDMI

text: TRUE

text: control blank

characteristic: sample type

text: metagenome

text: TRUE

text: cheese niche study


text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: blank

characteristic: alias

text: TRUE

text: Control

text: Negative

text: Sterile water blank

text: urban biome

text: research facility

text: misc environment

text: sterile water

text: USA:CA:San Diego

text: metagenome

text: UCSDMI

text: TRUE

text: control blank

text: metagenome

text: TRUE

text: cheese niche study


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF EXETER

text: public

tag: Namespace:UNIVERSITY OF EXETER

characteristic: collection date

text: Sequenced with LASL long reads and short reads

characteristic: description

text: Coastal marine virome

characteristic: environment (biome)

text: Marine

characteristic: environment (feature)

text: Seawater

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

text: VIrION

characteristic: project name

text: metagenome

text: Illumina Nextera and 2D Nanopore

characteristic: sequencing method

text: L4 virome sampled from the Western English Channel

characteristic: title

text: water

characteristic: water environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2D Nanopore reads

text: Coastal marine water

text: Marine water

text: Virome

text: United Kingdom

text: metagenome

text: metagenome

text: VirION

text: metagenome

text: minion Nanopore

text: Long reads for L4 virome

text: water


characteristic: ENA-CHECKLIST

text: AUSTRALIAN GENOME RESEARCH FACILITY

text: public

tag: Namespace:AUSTRALIAN GENOME RESEARCH FACILITY

text: house mouse

characteristic: common name

text: B6CastF1 E14.5 embryonic placenta

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: M

characteristic: sex

characteristic: strain

text: placenta

characteristic: tissue type


text: AUSTRALIAN GENOME RESEARCH FACILITY

text: public

text: house mouse

text: CastB6F1 E14.5 embryonic placenta

text: Mus musculus

characteristic: organism

text: Mus musculus

text: M

text: placenta

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: REGN

text: public

text: house mouse

text: mc38 tumor infiltrating T cell

text: Mus musculus

text: Mus musculus

text: T cell


characteristic: External Id

text: REGN

characteristic: INSDC center name

text: public

text: house mouse

text: splenic T cell in mc38 tumor bearing mouse

text: Mus musculus

text: Mus musculus

text: T cell


text: REGN

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: RNA-Seq of mc38 tumor infiltrating T cells

text: Mus musculus

text: Mus musculus

text: T cell


text: REGN

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: RNA-Seq of splenic T cells in mc38 tumor bearing mouse

text: Mus musculus

text: Mus musculus

text: T cell


characteristic: ENA-CHECKLIST

text: REGN

text: public

characteristic: Submitter Id

text: house mouse

text: RNA-Seq of splenic T cells in mouse subjected to 1 week lymphocytic choriomeningitis virus

text: Mus musculus

text: Mus musculus

text: T cell


text: REGN

text: public

text: house mouse

characteristic: common name

text: RNA-Seq of splenic T cells in na�ve mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: T cell


text: REGN

text: public

text: house mouse

text: targeted amplicon sequencing of splenic T cells in mouse subjected to 1 week lymphocytic choriomeningitis virus

text: Mus musculus

text: Mus musculus

text: T cell


text: REGN

text: public

text: house mouse

text: targeted amplicon sequencing of splenic T cells in na�ve mouse

text: Mus musculus

text: Mus musculus

text: T cell


text: Copadichromis borleyi

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Copadichromis borleyi

text: Copadichromis borleyi

text: Copadichromis borleyi

characteristic: subject id


text: Plasmodium falciparum

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: WAGENINGEN UNIVERSITY

text: public

tag: Namespace:WAGENINGEN UNIVERSITY

text: Veillonellaceae bacterium ALE

text: Veillonellaceae bacterium ALE

text: Genome sequencing and description of Lucifera butyrica gen. nov. sp. nov., an novel glycerol fermenter and sulfur respirer isolated from acid rock drainage


text: IRHS

text: public

text: Lydie Kerdraon

characteristic: collected by

characteristic: collection date

text: bread wheat

text: Soissons

characteristic: cultivar

text: V4 region of 16S rRNA gene associated to Crop residues of Wheat (Rotation)

text: France

characteristic: geographic location (country and/or sea)

text: Ile de France - Thiverval-Grignon

characteristic: geographic location (region and locality)

text: Crop residues

characteristic: isolation source

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (Rotation)


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRHS

characteristic: INSDC center name

text: public

text: Lydie Kerdraon

text: rape

text: Alpaga

text: V4 region of 16S rRNA gene associated to Crop residues of Oilseed Rape

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Brassica napus

characteristic: organism

text: Brassica napus

text: Crop residues of Oilseed Rape

characteristic: title


characteristic: ENA-CHECKLIST

text: IRHS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Lydie Kerdraon

text: bread wheat

text: Soissons

text: V4 region of 16S rRNA gene associated to Crop residues of Wheat (monoculture)

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (monoculture)


text: IRHS

text: public

text: Lydie Kerdraon

characteristic: collected by

characteristic: collection date

text: rape

characteristic: common name

text: Alpaga

text: ITS1 region and v4 region of 16S rRNA gene associated to Crop residues of Oilseed Rape

characteristic: description

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Brassica napus

text: Brassica napus

text: Crop residues of Oilseed Rape


text: IRHS

text: public

text: Lydie Kerdraon

text: bread wheat

text: Soissons

characteristic: cultivar

text: ITS1 region and v4 region of 16S rRNA gene associated to Crop residues of Wheat (monoculture)

text: France

characteristic: geographic location (country and/or sea)

text: Ile de France - Thiverval-Grignon

characteristic: geographic location (region and locality)

text: Crop residues

characteristic: isolation source

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (monoculture)


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRHS

characteristic: INSDC center name

text: public

text: Lydie Kerdraon

text: bread wheat

text: Soissons

text: ITS1 region and v4 region of 16S rRNA gene associated to Crop residues of Wheat (Rotation)

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (Rotation)


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: metagenome

characteristic: organism

text: metagenome

characteristic: title


text: IRHS

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Lydie Kerdraon

text: rape

text: Alpaga

text: ITS1 region associated to Crop residues of Oilseed Rape

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Brassica napus

text: Brassica napus

text: Crop residues of Oilseed Rape


text: IRHS

text: public

text: Lydie Kerdraon

characteristic: collected by

characteristic: collection date

text: bread wheat

characteristic: common name

text: Soissons

text: ITS1 region associated to Crop residues of Wheat (Rotation)

characteristic: description

text: France

text: Ile de France - Thiverval-Grignon

text: Crop residues

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (Rotation)


text: IRHS

text: public

text: Lydie Kerdraon

text: bread wheat

text: Soissons

characteristic: cultivar

text: ITS1 region associated to Crop residues of Wheat (monoculture)

text: France

characteristic: geographic location (country and/or sea)

text: Ile de France - Thiverval-Grignon

characteristic: geographic location (region and locality)

text: Crop residues

characteristic: isolation source

text: Triticum aestivum

text: Triticum aestivum

text: Crop residues of Wheat (monoculture)


LUCI

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WAGENINGEN UNIVERSITY

characteristic: INSDC center name

text: public

text: LUCI

text: Veillonellaceae bacterium ALE

text: Veillonellaceae bacterium ALE


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

tag: Namespace:Faculty of Medicine, Goethe-University Frankfurt

text: ArrayExpress

characteristic: broker name

text: macrophage

characteristic: cell type

text: human

text: Protocols: For coculture experiments, MΦ were cultured at a density of 5 x 105 cells/mL. MCF-7 cancer cells were detached from culture flasks using trypsin-EDTA and washed with PBS. Tumor cells were resuspended in MΦ media and cocultured with MΦ at the same density. After 48 h, residual MCF-7 cells were removed from the plates using trypsin-EDTA for 3-5 min, which left adherence of MΦ unaltered. Adhered macrophages were harvested and processed for RNA isolation according to mirVana protocol. Primary human monocytes (PBMCs) were isolated from buffy coats of anonymous healthy donors obtained from DRK Blutspendedienst using Ficoll-Hypaque gradients (PAA Laboratories). Cells were washed twice with PBS and seeded onto high-adherence culture dishes (Sarstedt, Nümbrecht, Germany). After culturing PBMCs for 1 h in RPMI 1640 supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin, non-adherent cells were washed away and remaining monocytes were cultured in media containing human serum for 7 days to allow the differentiation towards macrophages. Macrophages were either left untreated or cocultured with MCF-7 breast cancer cells for 48h. Additionally, naive macrophages were treated with 100 nM RvD1 for 6 h. We trimmed low quality regions and adapter sequences from the raw sequence reads with Trimmomatic. We first clipped leading and trailing positions with a base quality value below 3, and subsequently performed a sliding window analysis and clipped the read if a window of length 4 was encountered where the average base quality dropped below 15. Subsequently, we screened and removed adapters of the Illumina TrueSeq small RNA kit, and the DPN2 expression primer using the option ILLUMINACLIP:2:30:7 of Trimmomatic. In a second round of adapter clipping, we removed remaining short adapter sequences using the option ILLUMINACLIP:0:30:5. Sequence reads with a remaining length below 18 bp were discarded. MΦ were harvested for RNA isolation using the mirVanaTM miRNA isolation kit (ThermoFisher Scientific, MA, USA) according to the manufacturer’s instructions cDNA libraries from total RNA were generated using the TruSeq® Small RNA sample preparation protocol (Illumina, San Diego, USA). Enrichment and size distribution of the libraries were quality-assessed by capillary electrophoresis on a DNA high sensitive chip on Bioanalyzer (Agilent, Waldbronn, Germany).

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: MCF-7 co-culture

characteristic: stimulus

characteristic: title


text: Faculty of Medicine, Goethe-University Frankfurt

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: macrophage

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: macrophage

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Resolvin D1 (100 nanomolar)


characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: macrophage

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: MCF-7 co-culture


text: UNIVERSITY OF VIENNA

text: public

tag: Namespace:UNIVERSITY OF VIENNA

text: house mouse

text: day 14 postnatal

characteristic: dev stage

text: Mus musculus

text: Mus musculus

text: whole brain

characteristic: tissue type

text: Mice nascent-RNA seq


text: UNIVERSITY OF VIENNA

text: public

text: house mouse

text: Primary neuronal cultures were established from mouse embryos at embryonic day 11.5. Each embryo isolate was split into two wells of a 6-well dish coated with poly-D-lysine. Neuronal cells were subsequently cultured in neurobasal A medium (supplemented with B27, penicillin, streptomycin, gentamycin and L-glutamine) for 2 weeks. To prevent outgrowth of dividing cells, Cytosine β-D-arabinofuranoside (Ara-C) (Sigma Aldrich, MO) was added to 1 µM after 48 h. After 96 h the concentration was raised to 5 µM. For splicing inhibition, cells were treated with meayamycin at a concentration of 15 nM or DMSO as control.

text: embryonic day 11.5

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Primary neuronal cultures

text: Mice: primiary Neurons: splice inhibition


text: UNIVERSITY OF VIENNA

text: public

text: house mouse

text: one marrow cultures were established as described previously (Kroeger et al; J Vis Exp. 2009 Jan 6;(23). pii: 1026. doi: 10.3791/1026). Briefly, bone marrow was isolated from the femurs and tibiae of 6 week old mice. Subsequently, cells were transferred to RPMI medium (supplemented with 10% FBS, penicillin streptomycin, gentamycin and L-glutamine). Splicing inhibition was performed after 16 h of incubation using meayamycin at a concentration of 5 nM for 6 h. DMSO was used as a vehicle control.

text: 6 week old mice

text: Mus musculus

text: Mus musculus

text: bone marrow from the femurs and tibiae

text: Mice: splice inhibition: bone marrow


text: public

text: NOR

tag: Namespace:Department of Medical Microbiology, Stavanger University Hospital

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

characteristic: isolation source

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

type: AMR curated data - CABBAGE

value: ceftazidime

value: EUCAST

value: resistant

value: Klebsiella pneumoniae

value: cefotaxime

value: meropenem

value: susceptible

value: ciprofloxacin

value: trimethoprim-sulfamethoxazole

value: gentamicin


characteristic: ENA-FIRST-PUBLIC

text: Department of Medical Microbiology, Stavanger University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Norway

text: diseased

text: Homo sapiens

text: Blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

type: Other BioSample


text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

characteristic: host

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

characteristic: isolation source

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: public

text: NOR

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

characteristic: host

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

characteristic: isolation source

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: public

text: NOR

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

characteristic: host

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

characteristic: isolation source

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: public

text: NOR

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Blood

text: blood

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


text: public

text: Norway

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Urine

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

characteristic: host

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: public

text: NOR

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

characteristic: isolation source

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: INSDC first public

text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: intermediate


text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

characteristic: host

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

text: public

text: NOR

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

characteristic: isolation source

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: NOR

text: Klebsiella

text: Norway

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title


characteristic: INSDC first public

text: public

text: NOR

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Norway

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: diseased

text: Homo sapiens

text: Urine

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: FRAUNHOFER IGB

text: public

tag: Namespace:FRAUNHOFER IGB

text: Bgp 2; Substrates: maize silage, pig+cattle slurry, chicken dung, organic waste; Operating temperature: 54°C

characteristic: description

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

text: BioMETHA methagenome hybrid assembly

characteristic: project name

text: metagenome

text: Illumina HiSeq 2500

characteristic: sequencing method

text: Biogas plant 2 metagenomic WGS sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRAUNHOFER IGB

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bgp 3; Substrates: maize silage, grass silage; Operating temperature: 52.4°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 3 metagenomic WGS sample


text: FRAUNHOFER IGB

characteristic: INSDC first public

text: public

characteristic: collection date

text: Bgp 4; Substrates: maize silage, grass silage; Operating temperature: 40.3°C

characteristic: description

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 4 metagenomic WGS sample

characteristic: title


characteristic: ENA-CHECKLIST

text: FRAUNHOFER IGB

text: public

text: Bgp 5; Substrates: maize silage, cattle slurry, horse manure; Operating temperature: 51°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

text: BioMETHA methagenome hybrid assembly

characteristic: project name

text: metagenome

text: Illumina HiSeq 2500

characteristic: sequencing method

text: Biogas plant 5 metagenomic WGS sample


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRAUNHOFER IGB

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bgp 6; Substrates:maize silage; Operating temperature: 40°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 6 metagenomic WGS sample


text: FRAUNHOFER IGB

characteristic: INSDC first public

text: public

characteristic: collection date

text: Bgp 7; Substrates:maize silage; Operating temperature: 40°C

characteristic: description

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 7 metagenomic WGS sample

characteristic: title


Bgp2 RNA

characteristic: ENA-CHECKLIST

text: FRAUNHOFER IGB

text: public

text: Bgp2 RNA

text: Bgp 2 RNA; Substrates: maize silage, pig+cattle slurry, chicken dung, organic waste; Operating temperature: 54°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

text: BioMETHA methagenome hybrid assembly

characteristic: project name

text: metagenome

text: Illumina HiSeq 2500

characteristic: sequencing method

text: Biogas plant 2 metatranscriptomic sample


Bgp3 RNA

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRAUNHOFER IGB

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bgp3 RNA

characteristic: Submitter Id

text: Bgp 3 RNA; Substrates: maize silage, grass silage; Operating temperature: 52.4°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 3 metatranscriptomic sample


Bgp7 RNA

text: FRAUNHOFER IGB

characteristic: INSDC first public

text: public

text: Bgp7 RNA

characteristic: collection date

text: Bgp 7 RNA; Substrates:maize silage; Operating temperature: 40°C

characteristic: description

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: Illumina HiSeq 2500

text: Biogas plant 7 metatranscriptomic sample

characteristic: title


Bgp2 ONT

characteristic: ENA-CHECKLIST

text: FRAUNHOFER IGB

text: public

text: Bgp2 ONT

text: Bgp 2 ONT; Substrates: maize silage, pig+cattle slurry, chicken dung, organic waste; Operating temperature: 54°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

text: BioMETHA methagenome hybrid assembly

characteristic: project name

text: metagenome

text: ONT MinION

characteristic: sequencing method

text: Biogas plant 2 metagenomic MinION ONT sample


Bgp3 ONT

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRAUNHOFER IGB

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bgp3 ONT

characteristic: Submitter Id

text: Bgp 3 ONT; Substrates: maize silage, grass silage; Operating temperature: 52.4°C

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: ONT MinION

text: Biogas plant 3 metagenomic MinION ONT sample


Bgp4 ONT

text: FRAUNHOFER IGB

characteristic: INSDC first public

text: public

text: Bgp4 ONT

characteristic: collection date

text: Bgp 4 ONT; Substrates: maize silage, grass silage; Operating temperature: 40.3°C

characteristic: description

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: ONT MinION

text: Biogas plant 4 metagenomic MinION ONT sample

characteristic: title


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

text: Atlantic salmon

characteristic: common name

text: water

text: food

text: fish

text: Denmark

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: Salmo salar

characteristic: organism

text: COMPARE Food Metagenomic Ring Trial

characteristic: project name

text: Salmo salar

text: shotgun

characteristic: sequencing method

text: COMPARE Food Metagenomic Ring Trial


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FEDERAL INSTITUTE FOR RISK ASSESSMENT (BFR)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Atlantic salmon

text: water

text: food

text: fish food product

text: Denmark

text: metagenome

text: Salmo salar

text: COMPARE Food Metagenomic Ring Trial

text: Salmo salar

text: shotgun

text: COMPARE Food Metagenomic Ring Trial


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:CNRS UMR 9198 - The Institute for Integrative Biology of the Cell (I2BC)

text: ArrayExpress

characteristic: broker name

text: HeLa

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: HeLa cells induced to express M.EcoGII-LaminB1 or M.EcoGII alone for 24hours by supplementing growing medium with 1 microM Shield1. Genomic DNA was isolated using the Qiagen Blood & Cell Culture DNA Midi Kit (Genomic-tip 100/G) according to the manufacturer’s recommendations together with RNAse treatment (200 µg/ml of RNAseA (Sigma) and RNase Cocktail™ Enzyme Mix (Ambion) (2.5U/ml RnaseA ; 100U/ml RnaseT1) at 37°C for 1 hour). Double stranded genomic DNA (10 µg) was sheared into fragments of 200-400bp using Bioruptor Plus sonicator (Diagenode) - 300 µL of 33,3ng/µl in 1.5ml TPX tube; 40 cycles 30sec/60sec ON/OFF ; low power. The temperature was kept at 4°C (using the Diagenode Water cooler, Cat. No. BioAcc-Cool) for optimal shearing results. 1% of sample was taken as an input. Fragmented DNA was end-repaired and A-tailed according to manufacturer recommendations (NEBNext End-repair module (NEB #E6050), NEBNext A-tailing module (NEB #E6053)). Double Stranded TruSeq Illumina adapters were ligated (NEBNext Ligation Module (NEB #E6056). Then, samples were diluted in TE buffer up to 360 µl, denatured for 10 minutes at 95°C and snap cooled on ice for 10 minutes. Samples were supplemented with 10x m6AIP buffer (100mM Na-Phosphate buffer, pH 7.0; 3M NaCl; 0.5% Triton X-100), 2.5 µg m6A antibody (SYSY) and rotated overnight at 4ºC. Next 20 uL of protein A/G Dynabeads mix (Thermo Fisher Scientific), pre-blocked for 1 hour (PBS-0.5%BSA-0.1%Tween-20), was added and samples rotated at 4ºC for 3 hours. Beads were washed 4 times in 1 ml 1X m6AIP buffer. Beads and input samples were resuspended in 150 µl digestion buffer (50mM Tris, pH 8.0; 10mM EDTA; 0.5% SDS) containing 300 µg/ml proteinase K and incubated for 3 hours at 50°C with shaking. DNA was purified by phenol/chloroform/isoamyl alcohol extraction and resuspended in 21 µl milliQ water. IP efficiency was checked by qPCR using 1µL of IP and INPUT sample. DNA recovered from Immunoprecipitation was amplified (10 cycles) using KAPA Hifi DNA polymerase (Kapa Biosystems) using Illumina primers, and purified with AMPureXB beads (Beckmann Coulter).

characteristic: description

text: cervical adenocarcinoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: uterine cervix

characteristic: organism part

text: Homo sapiens

characteristic: title


tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CNRS UMR 9198 - The Institute for Integrative Biology of the Cell (I2BC)

text: public

text: ArrayExpress

text: HeLa

text: epithelial cell

text: human

text: cervical adenocarcinoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: uterine cervix

text: Homo sapiens


characteristic: External Id

text: FRAUNHOFER IGB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection date

text: Bgp 1; Substrates: maize silage, cattle slurry, horse+pig+cattle manure, organic waste; Operating temperature: 46°C

text: biogas production plant

characteristic: environment (biome)

text: Anaerobic digestion of organic waste

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

text: BioMETHA methagenome hybrid assembly

characteristic: project name

text: metagenome

text: Illumina HiSeq 2500

characteristic: sequencing method

text: Biogas plant 1 metagenomic WGS sample


Bgp1 ONT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: FRAUNHOFER IGB

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Bgp1 ONT

characteristic: Submitter Id

text: Bgp 1 ONT; Substrates: maize silage, cattle slurry, horse+pig+cattle manure, organic waste; Operating temperature: 46°C

characteristic: description

text: biogas production plant

text: Anaerobic digestion of organic waste

text: organic material

text: Germany

text: metagenome

text: metagenome

text: BioMETHA methagenome hybrid assembly

text: metagenome

text: ONT MinION

text: Biogas plant 1 metagenomic MinION ONT sample

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: VIB-CMN Antwerp

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:VIB-CMN Antwerp

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: oxford nanopore promethion

text: PromethION NA19240 rebasecalled using Guppy1.4.0


E-MTAB-6386:Sample 13

text: UNSW

text: public

text: E-MTAB-6386:Sample 13

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

characteristic: cell type

text: human

text: Protocols: PBMCs were separated from freshly-collected whole blood by density gradient centrifugation and stained with the following monoclonal antibodies: CD19-BV421 (Biolegend), IgD-PerCP- Cy5.5, CD27-PE-Cy7, CD38-APC, CD10-PECF594, CD3-APC-Cy7, IgG-BV605 (BD Biosciences) and viability dye eFluor780 (eBioscience) for 30 min on ice and, following two washes, cells were resuspended in PBS with 1% HI bovine serum for single cell sorting. Following exclusion of non-viable/CD3+ cells, individual transitional (CD19+, IgD+, CD27-,CD10+), mature naïve (CD19+, IgD+, CD27-, CD10-), switched memory (CD19+, IgD-, CD27+, IgG+) and plasmablast (CD19+, IgD-, CD27++, CD38++) were sorted directly into 96- well PCR plates (Eppendorf) using a FACS ARIA III (BD Biosciences). PCR plates were stored at -80 C for subsequent analysis. polyA tail Single cell RNA sequencing was performed with the Smart-Seq2 protocol as described by Picelli et al. (Picelli, et al., 2013; Picelli, et al., 2014) with the following modifications. Cell lysis buffer was prepared by adding 1μL Rnase inhibitor (Clontech, Mountain View, CA) to 19μl Triton X- 100 solution (0.2% v/v). Cells were sorted directly into 96-well plates containing 1μL lysis buffer, 0.5μL dNTP (10mM) and 0.5μL oligo-dT primer at 5uM. Reverse-transcription and PCR amplification were performed as described (Picelli, et al., 2013) with the following exceptions: reactions were performed at half volumes, the IS PCR primer was reduced to a 50nM final concentration and the number of PCR cycles increased to 28. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit (Illumina; San Diego, CA, USA) at one quarter of the recommend total volume

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: A

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD19+ IgD- CD27+ IgG+

characteristic: phenotype

text: Homo sapiens

text: female

characteristic: sex

text: OK

characteristic: single cell quality

text: Sample 13


E-MTAB-6386:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNSW

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6386:Sample 14

characteristic: Submitter Id

text: ArrayExpress

text: memory B cell

text: human

characteristic: description

text: adult

text: normal

text: A

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD19+ IgD- CD27+ IgG+

text: Homo sapiens

text: female

text: OK

text: Sample 14

characteristic: title


E-MTAB-6386:Sample 15

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNSW

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6386:Sample 15

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

characteristic: common name

text: adult

text: normal

text: A

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: CD19+ IgD- CD27+ IgG+

text: Homo sapiens

text: female

text: OK

text: Sample 15


E-MTAB-6386:Sample 16

text: UNSW

text: public

text: E-MTAB-6386:Sample 16

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: A

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: CD19+ IgD- CD27+ IgG+

characteristic: phenotype

text: Homo sapiens

text: female

characteristic: sex

text: OK

characteristic: single cell quality

text: Sample 16


E-MTAB-6386:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNSW

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6386:Sample 17

characteristic: Submitter Id

text: ArrayExpress

text: memory B cell

text: human

characteristic: description

text: adult

text: normal

text: A

text: Homo sapiens

text: Homo sapiens

text: blood

text: CD19+ IgD- CD27+ IgG+

text: Homo sapiens

text: female

text: OK

text: Sample 17

characteristic: title


E-MTAB-6386:Sample 18

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNSW

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-6386:Sample 18

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

characteristic: common name

text: adult

text: normal

text: A

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: CD19+ IgD- CD27+ IgG+

text: Homo sapiens

text: female

text: OK

text: Sample 18