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characteristic: sequencing method

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text: Staphylococcus aureus

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text: Staphylococcus aureus

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characteristic: INSDC center name

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text: public

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text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

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text: Whole genome sequencing of Staphylococcus aureus BPH2013

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characteristic: External Id

text: UNIMELB

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text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

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text: Staphylococcus aureus

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text: ILLUMINA

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text: prokaryote

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text: Staphylococcus aureus

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characteristic: External Id

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text: Staphylococcus aureus

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text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

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text: Human

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text: prokaryote

text: human clinical

text: Staphylococcus aureus

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text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

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text: Whole genome sequencing of Staphylococcus aureus BPH2081


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text: UNIMELB

text: public

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text: Australia

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text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2018

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text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

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text: Staphylococcus aureus

text: Staphylococcus aureus

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text: Whole genome sequencing of Staphylococcus aureus BPH2024


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text: Staphylococcus aureus

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characteristic: INSDC center name

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text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

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characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2094

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characteristic: External Id

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text: Staphylococcus aureus

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text: Whole genome sequencing of Staphylococcus aureus BPH2072


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text: Staphylococcus aureus

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characteristic: INSDC center name

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text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2004


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characteristic: External Id

text: UNIMELB

text: public

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characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2078

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text: UNIMELB

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text: Staphylococcus aureus

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text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2019


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characteristic: INSDC center name

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text: BRAEMBL

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text: Human

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text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2007


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text: UNIMELB

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text: Staphylococcus aureus

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text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus JKD6021


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characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

text: public

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text: Australia

text: Australia

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text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2015

characteristic: title


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characteristic: INSDC center name

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text: public

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text: Australia

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characteristic: investigation type

text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH0491


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text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH0488


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH0510

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characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

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text: Australia

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text: Human

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text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH0510


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text: UNIMELB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: BRAEMBL

text: Australia

text: Australia

text: Human

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text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2012

characteristic: title


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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIMELB

text: public

text: BRAEMBL

text: Australia

text: Australia

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characteristic: host

text: diseased

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text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2026


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characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

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text: BRAEMBL

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text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus JKD6051


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text: UNIMELB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: BRAEMBL

characteristic: broker name

characteristic: collection date

text: Australia

characteristic: country

characteristic: description

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2005


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH2011

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIMELB

text: public

text: BRAEMBL

text: Australia

text: Australia

text: Human

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text: prokaryote

text: human clinical

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2011

characteristic: title


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, JKD6121

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

text: public

text: BRAEMBL

text: Australia

text: Australia

text: Human

characteristic: host

text: diseased

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text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus JKD6121


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text: UNIMELB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: BRAEMBL

text: Australia

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus JKD6023


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH2031

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIMELB

text: public

text: BRAEMBL

characteristic: broker name

characteristic: collection date

text: Australia

characteristic: country

characteristic: description

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2031


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH2025

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

text: public

text: BRAEMBL

text: Australia

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

text: ILLUMINA

characteristic: sequencing method

characteristic: strain

text: Whole genome sequencing of Staphylococcus aureus BPH2025

characteristic: title


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH0503

text: UNIMELB

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: BRAEMBL

text: Australia

text: Australia

text: Human

characteristic: host

text: diseased

characteristic: host status

text: prokaryote

characteristic: investigation type

text: human clinical

characteristic: isolation source

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH0503


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH2088

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIMELB

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: BRAEMBL

text: Australia

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2088


name: UoM, Convergent Adaptation in the Dominant Global Hospital Clone ST239 of Methicillin-Resistant Staphylococcus aureus, BPH2014

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIMELB

text: public

text: BRAEMBL

characteristic: broker name

characteristic: collection date

text: Australia

characteristic: country

characteristic: description

text: Australia

text: Human

text: diseased

text: prokaryote

text: human clinical

text: Staphylococcus aureus

text: Staphylococcus aureus

text: ILLUMINA

text: Whole genome sequencing of Staphylococcus aureus BPH2014


text: Staphylococcus aureus

characteristic: ArrayExpress-Species

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Staphylococcus aureus

characteristic: organism

text: Ewan

characteristic: sample description

text: Staphylococcus aureus


text: Staphylococcus aureus

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Staphylococcus aureus

text: Pemba Tanzania

text: Staphylococcus aureus


text: Staphylococcus aureus

characteristic: External Id

text: SC

text: public

text: Staphylococcus aureus

text: Qena_Egypt cattle

text: Staphylococcus aureus


text: Staphylococcus aureus

text: SC

characteristic: INSDC last update

text: public

text: Staphylococcus aureus

text: Shalateen_Egypt camel

text: Staphylococcus aureus


text: Staphylococcus sp.

text: SC

text: public

characteristic: INSDC status

text: Staphylococcus sp.

text: Qena_Egypt cattle

text: Staphylococcus sp.


text: Staphylococcus sp.

text: SC

text: public

characteristic: SRA accession

text: Staphylococcus sp.

text: Shalateen_Egypt camel

text: Staphylococcus sp.


text: Staphylococcus aureus

text: SC

text: public

characteristic: Submitter Id

text: Staphylococcus aureus

text: Bovine

text: Staphylococcus aureus


text: Staphylococcus sp.

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Staphylococcus sp.

text: Bovine

text: Staphylococcus sp.


text: Haemophilus parasuis

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: [Haemophilus] parasuis

text: [Haemophilus] parasuis

characteristic: strain


text: Staphylococcus aureus

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

characteristic: broker name

text: Mycobacterium

characteristic: genus

characteristic: isolate

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABQ00029615_S20. CTB/NICD. BQ00029615_S20

characteristic: title

type: AMR curated data - CABBAGE

value: ethambutol

value: susceptible

value: Mycobacterium tuberculosis

value: rifampin

value: isoniazid

value: pyrazinamide


text: 34414415; 34461978; 35780211

text: CTB/NICD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0074450_S32. CTB/NICD. TRL0074450_S32

value: resistant


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: SACG01334682_S2. CTB/NICD. CG01334682_S2


text: 34414415; 34461978; 35780211

characteristic: ENA-LAST-UPDATE

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0022086_S15. CTB/NICD. TRL0022086_S15


text: 34414415; 34461978; 35780211

characteristic: External Id

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SAED00874424_S22. CTB/NICD. ED00874424_S22


text: 34414415; 34461978; 35780211

text: CTB/NICD

characteristic: INSDC last update

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0115412_S22. CTB/NICD. TRL0115412_S22


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

characteristic: INSDC status

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0057671_S18. CTB/NICD. TRL0057671_S18


text: Staphylococcus aureus

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: SRA accession

text: Staphylococcus aureus

text: Human England

characteristic: sample description

text: Staphylococcus aureus


text: unidentified

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: Submitter Id

text: unidentified

text: unidentified


text: Staphylococcus aureus

text: MSSA

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: MSSA

characteristic: strain


text: Staphylococcus aureus

text: MRSA

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: MRSA


text: Staphylococcus aureus

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus


text: CGS-GL

text: public

characteristic: description

text: Escherichia coli

text: Escherichia coli


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

characteristic: broker name

text: Mycobacterium

characteristic: genus

characteristic: isolate

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SAUJ00125495_S24. CTB/NICD. UJ00125495_S24

characteristic: title


text: 34414415; 34461978; 35780211

characteristic: ENA-LAST-UPDATE

text: CTB/NICD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: SABT00026048_S12. CTB/NICD. BT00026048_S12


text: 33347430; 34414415; 34461978; 35780211

characteristic: External Id

text: CTB/NICD

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABK00092684_S23. CTB/NICD. BK00092684_S23


text: 34414415; 34461978; 35780211

characteristic: ENA-CHECKLIST

text: CTB/NICD

text: public

text: ZAF

characteristic: SRA accession

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SACE01825534_S2. CTB/NICD. CE01825534_S2


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

characteristic: Submitter Id

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABE01210097_S15. CTB/NICD. BE01210097_S15


text: 34414415; 34461978; 35780211

characteristic: ENA-FIRST-PUBLIC

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SADH00659004_S17. CTB/NICD. DH00659004_S17


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0085451_S24. CTB/NICD. TRL0085451_S24


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0115652_S40. CTB/NICD. TRL0115652_S40


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SAJA00214410_S4. CTB/NICD. JA00214410_S4


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABF01114045_S1. CTB/NICD. BF01114045_S1


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABK00082371_S21. CTB/NICD. BK00082371_S21


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0114825_S19. CTB/NICD. TRL0114825_S19


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0115400_S13. CTB/NICD. TRL0115400_S13


text: CGS-GL

text: public

text: mouse gut metagenome

text: mouse gut metagenome


text: INRA-CMSE

text: public

text: low

characteristic: Level of intensification of land-use

unit: g

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

unit: m

characteristic: depth

text: Long term abandoned grassland (26-29 years)

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACGTAGATC

characteristic: multiplex identifiers

text: soil metagenome

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_2.0min; 35 cycles of denaturation: 94degC_0.5min;annealing: 52degC_0.5min;elongation: 72degC_1.0min;final elongation: 72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.

characteristic: pcr conditions

text: F479 :5-CAGCMGCYGCNGTAANAC-3 ;R888 :5-CCGYCAATTCMTTTRAGT-3

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

unit: % sand/silt/clay

characteristic: texture

characteristic: total n

unit: g/kg

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTCTAGTAC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Medium term abandoned grassland (16-23 years)

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGCGTATACA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CAGTAGACGT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTACAGTCA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Short term abandoned grassland (6-9 years)

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTAGACTAG

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CACGCTACGT

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTCTAGTAC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: cork harvest

characteristic: current land use

text: forest

characteristic: current vegetation

characteristic: depth

text: Woodland

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CATAGTAGTG

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: cork harvest

text: forest

text: Woodland

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGACGTGACT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: cork harvest

characteristic: current land use

text: forest

characteristic: current vegetation

characteristic: depth

text: Woodland

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CATACTCTAC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: mixed forest-grassland

text: Wooded pasture grazed and periodically (5-10 years) cultivated to grow annual forage crops

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTGTCTCTA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture + annual forage crops

characteristic: current land use

text: mixed forest-grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CACGTAGATC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: grassland

text: Intensive grassland grazed and periodically (1-5 years) cultivated to grow annual forage crops

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGACGTGACT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture + annual forage crops

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTAGACTAG

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: grassland

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACGTGTCGC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Natural grassland grazed

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGATCGTATA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Natural grassland grazed

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CAGTAGACGT

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed and fertilized

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CATAGTAGTG

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Natural grassland grazed and fertilized

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGCGTATACA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed and fertilized

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CATACTCTAC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Cultivated grassland (tillage + seeding), grazed and fertilized

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGAGACGCGC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCGTATA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CACGTGTCGC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CAGACGTCTG

characteristic: multiplex identifiers

text: soil metagenome

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3.0min; 35 cycles of denaturation: 94degC_1.0min; annealing: 52degC_1.0min;elongation: 72degC_1.0min;final elongation:72degC_5.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.

characteristic: pcr conditions

text: FR1 :5-ANCCATTCAATCGGTANT-3 ; FF390 :5-CGATAACGAACGAGACCT-3

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTACAGTCA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGCGACATCT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CATAGTGACA

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title