text: MPI-EVA

text: public

text: human

text: sample GV09 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPI-EVA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: sample GV10 from ethnicity Desano

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


text: MPI-EVA

text: public

text: human

text: sample GV15 from ethnicity Yuruti

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV19 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV21 from ethnicity Wanano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV22 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV23 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV24 from ethnicity Wanano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV26 from ethnicity Wanano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV28 from ethnicity Tukano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV29 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV30 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GV34 from ethnicity Desano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG01 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG03 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG05 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG06 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG07 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG08 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG09 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG10 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG12 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG19 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG26 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG28 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG29 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample GVG30 from ethnicity Guayabero

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET191 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET192 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET193 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET194 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET195 from ethnicity Achagua

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET196 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET197 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET199 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET201 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET202 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET204 from ethnicity Achagua

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET205 from ethnicity Achagua

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET206 from ethnicity Achagua

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET207 from ethnicity Achagua

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET208 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET209 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET210 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET211 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET212 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET213 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET214 from ethnicity Piapoco

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET215 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET216 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET217 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET218 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET219 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET220 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET222 from ethnicity Mestizo

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET224 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET225 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET226 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET229 from ethnicity Mestizo

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET230 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample MET231 from ethnicity Saliba

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT274 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT279 from ethnicity Mestizo

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT280 from ethnicity Uitoto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT281 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT282 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT283 from ethnicity Kamentsa

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT288 from ethnicity Kamentsa

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT289 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT294 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT295 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT296 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT297 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT298 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT300 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT305 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT306 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT307 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT308 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT309 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT310 from ethnicity Inga

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT317 from ethnicity Kamentsa

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT319 from ethnicity Cofan

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT321 from ethnicity Pasto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT324 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT325 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT327 from ethnicity Uitoto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT329 from ethnicity Mestizo

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT330 from ethnicity Cofan

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT331 from ethnicity Kamentsa

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT332 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT334 from ethnicity Cofan

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT336 from ethnicity Uitoto

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT337 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT338 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT339 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT342 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT346 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT347 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT348 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT349 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT350 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT351 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT353 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT355 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT357 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT360 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT362 from ethnicity Siona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT366 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT369 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT370 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT371 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT372 from ethnicity Carijona

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT374 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT375 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT376 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT377 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT378 from ethnicity Guambiano

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT382 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT384 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT385 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT386 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT387 from ethnicity Murui

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT388 from ethnicity Mestizo

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT389 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT390 from ethnicity Coreguaje

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT392 from ethnicity Nasa

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT394 from ethnicity Cofan

text: Homo sapiens

text: Homo sapiens


text: MPI-EVA

text: public

text: human

text: sample PUT395 from ethnicity Cofan

text: Homo sapiens

text: Homo sapiens


text: LAVAL UNIVERSITY

text: public

tag: Namespace:LAVAL UNIVERSITY

characteristic: collection date

text: rat

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: rat gut metagenome

characteristic: project name

text: rat gut metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Biliopancreatic Diversion With Duodenal Switch alters bacterial diversity and richness in Wistar rats�


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Medical Univeristy of Graz

unit: kg

characteristic: absolute air humidity

unit: ºC

characteristic: air temperature

text: wood framed

characteristic: building occupancy type

text: rural

characteristic: building setting

text: built environment

characteristic: built environment environmental package

unit: µmol/L

characteristic: carbon dioxide

text: indoor

text: didactic showcase

text: indoor environment

text: uncontrolled

text: polymer concrete tiles wood

text: ultraviolet air treatment

characteristic: filter type

text: Germany

text: wood stove

characteristic: heating and cooling system type

text: foyer

characteristic: indoor space

text: metagenome

text: electric light

characteristic: light type

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

characteristic: organism count

text: Comparative metagenomics of controlled and uncontrolled built environments

characteristic: relative air humidity

text: indoor metagenome

text: Illumina

text: typically unoccupied

characteristic: space typical state

text: L

characteristic: typical occupant density

text: natural ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

text: wood framed

text: rural

text: built environment

characteristic: collection date

text: indoor

characteristic: common name

text: didactic showcase

characteristic: description

text: indoor environment

characteristic: environment (biome)

text: uncontrolled

characteristic: environment (feature)

text: polymer concrete tiles wood

characteristic: environment (material)

text: ultraviolet air treatment

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: wood stove

text: foyer

text: metagenome

characteristic: investigation type

text: electric light

text: indoor metagenome

characteristic: organism

characteristic: project name

text: indoor metagenome

text: Illumina

characteristic: sequencing method

text: typically unoccupied

text: L plus

characteristic: title

text: natural ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: air temperature

text: school

characteristic: building occupancy type

text: rural

characteristic: building setting

text: built environment

characteristic: built environment environmental package

characteristic: carbon dioxide

text: indoor

text: project group area

text: indoor environment

text: uncontrolled

text: tiles wood

text: ultraviolet air treatment

characteristic: filter type

text: Germany

text: heat pump

characteristic: heating and cooling system type

text: bathroom

characteristic: indoor space

text: metagenome

text: electric light

characteristic: light type

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

characteristic: organism count

characteristic: relative air humidity

text: indoor metagenome

text: Illumina

text: typically unoccupied

characteristic: space typical state

text: P

characteristic: typical occupant density

text: natural ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

text: school

text: rural

text: built environment

characteristic: collection date

text: indoor

characteristic: common name

text: project group area

characteristic: description

text: indoor environment

characteristic: environment (biome)

text: uncontrolled

characteristic: environment (feature)

text: tiles wood

characteristic: environment (material)

text: ultraviolet air treatment

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

text: bathroom

text: metagenome

characteristic: investigation type

text: electric light

text: indoor metagenome

characteristic: organism

characteristic: project name

text: indoor metagenome

text: Illumina

characteristic: sequencing method

text: typically unoccupied

text: P plus

characteristic: title

text: natural ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: air temperature

text: residential

characteristic: building occupancy type

text: rural

characteristic: building setting

text: built environment

characteristic: built environment environmental package

characteristic: carbon dioxide

text: indoor

text: foresters house

text: indoor environment

text: uncontrolled

text: polymer tiles stone

text: ultraviolet air treatment

characteristic: filter type

text: Germany

text: heat pump

characteristic: heating and cooling system type

text: kitchen

characteristic: indoor space

text: metagenome

text: electric light

characteristic: light type

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

characteristic: organism count

characteristic: relative air humidity

text: indoor metagenome

text: Illumina

text: typically occupied

characteristic: space typical state

text: F

characteristic: typical occupant density

text: natural ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

text: residential

text: rural

text: built environment

characteristic: collection date

text: indoor

characteristic: common name

text: foresters house

characteristic: description

text: indoor environment

characteristic: environment (biome)

text: uncontrolled

characteristic: environment (feature)

text: polymer tiles stone

characteristic: environment (material)

text: ultraviolet air treatment

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: heat pump

text: kitchen

text: metagenome

characteristic: investigation type

text: electric light

text: indoor metagenome

characteristic: organism

characteristic: project name

text: indoor metagenome

text: Illumina

characteristic: sequencing method

text: typically occupied

text: F plus

characteristic: title

text: natural ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: air temperature

text: health care

characteristic: building occupancy type

text: suburban

characteristic: building setting

text: built environment

characteristic: built environment environmental package

characteristic: carbon dioxide

text: indoor

text: Floor ICU microbiome

text: indoor environment

text: controlled

text: polymer furnished wood metals

text: HEPA filter

characteristic: filter type

text: Austria

text: forced air system

characteristic: heating and cooling system type

text: hallway

characteristic: indoor space

text: metagenome

text: electric light

characteristic: light type

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

characteristic: organism count

characteristic: relative air humidity

text: indoor metagenome

text: Illumina

text: typically occupied

characteristic: space typical state

text: ICU

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: absolute air humidity

text: industrial

text: urban

text: built environment

characteristic: collection date

text: indoor

characteristic: common name

text: gowning area

characteristic: description

text: indoor environment

characteristic: environment (biome)

text: controlled

characteristic: environment (feature)

text: polymer antistatic dissipative epoxy resin

characteristic: environment (material)

text: HEPA filter

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: forced air system

text: locker room

text: metagenome

characteristic: investigation type

text: electric light

text: indoor metagenome

characteristic: organism

characteristic: project name

text: indoor metagenome

text: Illumina

characteristic: sequencing method

text: typically occupied

text: UR

characteristic: title

text: mechanical ventilation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical Univeristy of Graz

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: air temperature

text: industrial

characteristic: building occupancy type

text: urban

characteristic: building setting

text: built environment

characteristic: built environment environmental package

characteristic: carbon dioxide

text: indoor

text: CR ISO8

text: indoor environment

text: controlled

text: HEPA filter

characteristic: filter type

text: Italy

text: forced air system

characteristic: heating and cooling system type

text: hallway

characteristic: indoor space

text: metagenome

text: electric light

characteristic: light type

characteristic: occupancy at sampling

characteristic: occupant density at sampling

text: indoor metagenome

characteristic: organism count

characteristic: relative air humidity

text: indoor metagenome

text: Illumina

text: typically occupied

characteristic: space typical state

characteristic: typical occupant density

text: mechanical ventilation

characteristic: ventilation type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BASEL, SWITZERLAND

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF BASEL, SWITZERLAND

characteristic: Submitter Id

characteristic: collection date

text: USA

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: Homo sapiens sapiens

characteristic: host scientific name

characteristic: isolate

text: Human sputum

characteristic: isolation source

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0004

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0031


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0052


text: UNIVERSITY OF BASEL, SWITZERLAND

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0054


text: UNIVERSITY OF BASEL, SWITZERLAND

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: China

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0069


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0072


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

characteristic: collection date

text: Gambia

characteristic: geographic location (country and/or sea)

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0091


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

characteristic: host health state

text: Homo sapiens sapiens

characteristic: host scientific name

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0136


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

characteristic: isolate

text: Human sputum

characteristic: isolation source

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0145


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0155

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: USA

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N0157


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: Ghana

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1176


text: UNIVERSITY OF BASEL, SWITZERLAND

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Ghana

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1201


text: UNIVERSITY OF BASEL, SWITZERLAND

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Ghana

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1202


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Ghana

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1216


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

characteristic: collection date

text: Sierra Leone

characteristic: geographic location (country and/or sea)

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1268


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: Germany

text: not collected

characteristic: host health state

text: Homo sapiens sapiens

characteristic: host scientific name

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1272


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: Germany

text: not collected

text: Homo sapiens sapiens

characteristic: isolate

text: Human sputum

characteristic: isolation source

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1274


text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: Germany

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N1283

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BASEL, SWITZERLAND

text: public

text: Australia

text: not collected

text: Homo sapiens sapiens

text: Human sputum

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis N3913


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE ROSLIN INSTITUTE

text: public

tag: Namespace:THE ROSLIN INSTITUTE

text: gyrfalcon

characteristic: common name

text: Gyr falcon

characteristic: description

text: Falco rusticolus

text: Falco rusticolus

text: Gyr falcon


text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: house mouse

text: Mus musculus

text: Mus musculus

characteristic: subject id


E-MTAB-7009:Sample 27

text: Wageningen University & Reseach

text: public

characteristic: SRA accession

text: E-MTAB-7009:Sample 27

tag: Namespace:Wageningen University & Reseach

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: After steady state was reached, 8mL samples were taken for RNA extraction (approximately 10 mgDW). Cells were immediately collected by centrifugation (4255 xg, 0°C for 5 min), supernatant was discarded and pellets were frozen in liquid nitrogen and stored at -80°C until further extraction. Samples for RNA extraction were taken in intervals of 1h for WT or every 3h for slm1. 16:8h diurnal cycle adapted cells RNA extraction was performed using the Maxwell® 16 LEV simplyRNA Tissue kit (Promega). Frozen algae pellet (≈200 μL) were submerged in 400 μL of homogenizing buffer supplemented with 8 μL 1-thioglycerol in a 2 mL Lysing matrix C tube (MP), prefilled with a mix of glass beads. Samples were disrupted using a FastPrep-24 instrument (MP). After disruption, all liquid was transferred to a LEV RNA Cartridge. 300 μL lysis buffer were added and the rest of the extraction was performed using a Maxwell MDx AS3000 machine (Promega) following manufacturer’s instructions. RNA integrity and quantity were assessed with an Experion system (Bio-Rad), and only high quality samples (RIN value ≥ 7) were selected. Random library

text: slm1 homozygous mutant

characteristic: genotype

text: Diurnal cycle 16:8h

characteristic: growth condition

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

characteristic: strain

text: Sample 27


E-MTAB-7009:Sample 28

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 28

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

characteristic: organism

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 28

characteristic: title


E-MTAB-7009:Sample 35

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 35

text: ArrayExpress

characteristic: description

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 35


E-MTAB-7009:Sample 36

text: Wageningen University & Reseach

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-7009:Sample 36

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 36


E-MTAB-7009:Sample 39

text: Wageningen University & Reseach

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-7009:Sample 39

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 39


E-MTAB-7009:Sample 66

text: Wageningen University & Reseach

text: public

characteristic: SRA accession

text: E-MTAB-7009:Sample 66

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 66


E-MTAB-7009:Sample 67

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 67

characteristic: Submitter Id

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 67


E-MTAB-7009:Sample 74

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 74

text: ArrayExpress

characteristic: broker name

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 74


E-MTAB-7009:Sample 75

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 75

text: ArrayExpress

text: slm1 homozygous mutant

characteristic: genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 75


E-MTAB-7009:Sample 1

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 1

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

characteristic: growth condition

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 1


E-MTAB-7009:Sample 2

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 2

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

characteristic: strain

text: Sample 2


E-MTAB-7009:Sample 20

characteristic: ENA-CHECKLIST

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 20

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 20


E-MTAB-7009:Sample 21

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 21

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 21


E-MTAB-7009:Sample 22

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 22

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

characteristic: organism

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 22


E-MTAB-7009:Sample 23

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 23

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 23

characteristic: title


E-MTAB-7009:Sample 24

characteristic: ENA-LAST-UPDATE

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 24

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 24


E-MTAB-7009:Sample 25

characteristic: External Id

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 25

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 25


E-MTAB-7009:Sample 26

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 26

text: ArrayExpress

characteristic: description

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 26


E-MTAB-7009:Sample 40

text: Wageningen University & Reseach

characteristic: INSDC center name

text: public

text: E-MTAB-7009:Sample 40

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 40


E-MTAB-7009:Sample 41

text: Wageningen University & Reseach

characteristic: INSDC first public

text: public

text: E-MTAB-7009:Sample 41

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 41


E-MTAB-7009:Sample 59

text: Wageningen University & Reseach

characteristic: INSDC last update

text: public

text: E-MTAB-7009:Sample 59

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 59


E-MTAB-7009:Sample 60

text: Wageningen University & Reseach

text: public

characteristic: INSDC status

text: E-MTAB-7009:Sample 60

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 60


E-MTAB-7009:Sample 61

text: Wageningen University & Reseach

text: public

characteristic: SRA accession

text: E-MTAB-7009:Sample 61

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 61


E-MTAB-7009:Sample 62

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 62

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 62


E-MTAB-7009:Sample 63

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 63

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 63


E-MTAB-7009:Sample 64

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 64

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 64


E-MTAB-7009:Sample 65

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 65

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

characteristic: growth condition

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 65


E-MTAB-7009:Sample 29

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 29

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

characteristic: strain

text: Sample 29


E-MTAB-7009:Sample 30

characteristic: ENA-CHECKLIST

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 30

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 30


E-MTAB-7009:Sample 31

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 31

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 31


E-MTAB-7009:Sample 32

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 32

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

characteristic: organism

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 32


E-MTAB-7009:Sample 33

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 33

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 33

characteristic: title


E-MTAB-7009:Sample 34

characteristic: ENA-LAST-UPDATE

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 34

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 34


E-MTAB-7009:Sample 68

characteristic: External Id

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 68

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 68


E-MTAB-7009:Sample 69

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 69

text: ArrayExpress

characteristic: description

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 69


E-MTAB-7009:Sample 70

text: Wageningen University & Reseach

characteristic: INSDC center name

text: public

text: E-MTAB-7009:Sample 70

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 70


E-MTAB-7009:Sample 71

text: Wageningen University & Reseach

characteristic: INSDC first public

text: public

text: E-MTAB-7009:Sample 71

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 71


E-MTAB-7009:Sample 72

text: Wageningen University & Reseach

characteristic: INSDC last update

text: public

text: E-MTAB-7009:Sample 72

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 72


E-MTAB-7009:Sample 73

text: Wageningen University & Reseach

text: public

characteristic: INSDC status

text: E-MTAB-7009:Sample 73

text: ArrayExpress

text: slm1 homozygous mutant

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 73


E-MTAB-7009:Sample 10

text: Wageningen University & Reseach

text: public

characteristic: SRA accession

text: E-MTAB-7009:Sample 10

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 10


E-MTAB-7009:Sample 11

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 11


E-MTAB-7009:Sample 12

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 12

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 12


E-MTAB-7009:Sample 13

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 13

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 13


E-MTAB-7009:Sample 14

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 14

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

characteristic: growth condition

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 14


E-MTAB-7009:Sample 15

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 15

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

characteristic: strain

text: Sample 15


E-MTAB-7009:Sample 16

characteristic: ENA-CHECKLIST

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 16

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 16


E-MTAB-7009:Sample 17

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 17

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 17


E-MTAB-7009:Sample 18

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 18

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

characteristic: organism

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 18


E-MTAB-7009:Sample 19

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 19

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 19

characteristic: title


E-MTAB-7009:Sample 3

characteristic: ENA-LAST-UPDATE

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 3

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 3


E-MTAB-7009:Sample 4

characteristic: External Id

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 4

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 4


E-MTAB-7009:Sample 42

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 42

text: ArrayExpress

characteristic: description

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 42


E-MTAB-7009:Sample 43

text: Wageningen University & Reseach

characteristic: INSDC center name

text: public

text: E-MTAB-7009:Sample 43

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 43


E-MTAB-7009:Sample 44

text: Wageningen University & Reseach

characteristic: INSDC first public

text: public

text: E-MTAB-7009:Sample 44

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 44


E-MTAB-7009:Sample 45

text: Wageningen University & Reseach

characteristic: INSDC last update

text: public

text: E-MTAB-7009:Sample 45

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 45


E-MTAB-7009:Sample 46

text: Wageningen University & Reseach

text: public

characteristic: INSDC status

text: E-MTAB-7009:Sample 46

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 46


E-MTAB-7009:Sample 47

text: Wageningen University & Reseach

text: public

characteristic: SRA accession

text: E-MTAB-7009:Sample 47

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 47


E-MTAB-7009:Sample 48

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 48

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 48


E-MTAB-7009:Sample 49

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 49

text: ArrayExpress

characteristic: broker name

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 49


E-MTAB-7009:Sample 5

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 5

text: ArrayExpress

text: wild type genotype

characteristic: genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 5


E-MTAB-7009:Sample 50

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 50

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

characteristic: growth condition

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 50


E-MTAB-7009:Sample 51

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 51

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

characteristic: strain

text: Sample 51


E-MTAB-7009:Sample 52

characteristic: ENA-CHECKLIST

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 52

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 52


E-MTAB-7009:Sample 53

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 53

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 53


E-MTAB-7009:Sample 54

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 54

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

characteristic: organism

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 54


E-MTAB-7009:Sample 55

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 55

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 55

characteristic: title


E-MTAB-7009:Sample 56

characteristic: ENA-LAST-UPDATE

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 56

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 56


E-MTAB-7009:Sample 57

characteristic: External Id

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 57

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 57


E-MTAB-7009:Sample 58

text: Wageningen University & Reseach

text: public

text: E-MTAB-7009:Sample 58

text: ArrayExpress

characteristic: description

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 58


E-MTAB-7009:Sample 6

text: Wageningen University & Reseach

characteristic: INSDC center name

text: public

text: E-MTAB-7009:Sample 6

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 6


E-MTAB-7009:Sample 7

text: Wageningen University & Reseach

characteristic: INSDC first public

text: public

text: E-MTAB-7009:Sample 7

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 7


E-MTAB-7009:Sample 8

text: Wageningen University & Reseach

characteristic: INSDC last update

text: public

text: E-MTAB-7009:Sample 8

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 8


E-MTAB-7009:Sample 9

text: Wageningen University & Reseach

text: public

characteristic: INSDC status

text: E-MTAB-7009:Sample 9

text: ArrayExpress

text: wild type genotype

text: Diurnal cycle 16:8h

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Tetradesmus obliquus

text: Sample 9


text: fecal metagenome

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: SRA accession

text: fecal metagenome

characteristic: common name

text: feces metagenome

text: feces metagenome

characteristic: subject id


text: public

tag: Namespace:MRC Institute of Genetics and Molecular Medicine, University of Edinburgh

characteristic: Submitter Id

text: common wombat

text: Affected by sarcoptic mange

text: Australia

characteristic: geographic location (country and/or sea)

text: Tasmania

characteristic: geographic location (region and locality)

text: Scott Carver

characteristic: identified by

text: Vombatus ursinus

text: Vombatus ursinus

text: Male

characteristic: sex

text: spleen

characteristic: tissue type

text: Bruce the wombat WM1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Philipps-University Marburg, Genomics Core Facility

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: All crosses were kept on standard medium on 25°C for w1118, tbrd-11 homozygous mutants and ∆tplus3a/b/Df(2L)BSC151. Testes were dissected in PBS, transferred to Trizol and snap frozen in liquid nitrogen, then stored on -80°C. Flies were kept on 25°C on standard Drosophila medium. Total RNA from 100 Testes was isolated using Trizol (Invitrogen). Total RNA from 200 Testes was pooled and digested with Turbo DNAse I (Ambion) and then purified with RNeasy mini Kit (Qiagen). Prior to library preparation RNA quality was assessed using the Experion RNA StdSens Analysis Kit (BioRad). RNASeq libraries were prepared from total RNA using the TruSeq Stranded mRNA LT kit (Illumina) according to the manufacturer’s instructions. Quality of sequencing libraries was controlled on a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent).

text: adult

characteristic: developmental stage

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: tbrd-1 homozygous knockout

characteristic: phenotype

text: Drosophila melanogaster

text: male

characteristic: strain

characteristic: title


characteristic: External Id

text: Philipps-University Marburg, Genomics Core Facility

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: tbrd-1 homozygous knockout

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: wild type phenotype

text: Drosophila melanogaster

text: male

characteristic: sex


text: public

text: E-MTAB-7013:tplus3a/b/Df(2L)BSC151_sample_1

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: tplus3a/b and tsh triple homozygous knockout

characteristic: phenotype

text: Drosophila melanogaster

text: male


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-7013:tplus3a/b/Df(2L)BSC151_sample_2

text: ArrayExpress

text: fruit fly

text: adult

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male

characteristic: strain

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-7013:tplus3a/b/Df(2L)BSC151_sample_3

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

characteristic: common name

text: adult

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: KAP1 antibody, ab22553 (abcam), was used in inducible SMARCAD1 knockdown cells prior to and after two day induction of SMARCAD1 knockdown as well as in SMARCAD1 knockdown cells that express a FLAG tagged SMARCAD1 transgene; either wild-type or an ATPase mutant. FLAG antibody used was F1804 (Sigma) in SMARCAD1 knockdown cells expressing no FLAG tagged protein (negative control) and in cells expressing FLAG tagged SMARCAD1; either wild-type or an ATPase mutant. Mouse E14 ES cells were cultivated feeder-cell free on gelatin-coated dishes in Dulbecco's Modified Eagle Medium (DMEM, Life Technologies) supplemented with 15% foetal calf serum, 2 mM L-glutamine, 1x non-essential amino acids, 50 μM 2-mercaptoethanol, penicillin/streptomycin and 1000U/ml ESGRO leukemia inhibitory factor. To generate an enzymatically inactive SMARCAD1 chromatin remodeler a mutation in the ATP binding pocket (K523R) was generated by site-directed mutagenesis using the QuikChange Lighting Site Directed Mutagenesis Kit (Agilent 210518) and the following oligos: Fwd 5’-GCAGACGAAATGGGCCTAGGAAGAACCATTCAAGCCATTGC -3’ Rev 5’- GCAATGGCTTGAATGGTTCTTCCTAGGCCCATTTCGTCTGC-3’. A Triple-FLAG tagged SMARCAD1 wild-type (E14 S-KD.WT) and ATPase mutant (E14 S-KD.ATPase_mt) constructs were stably integrated into E14 cells carrying a doxycycline inducible shRNA (E14.) targeting the 3’UTR of SMARCAD1 (shSMARCAD1) as described (Ding et al., 2017) prior to knocking down the endogenous SMARCAD1 (E14 S-KD). Doxycycline (0.5µg/ml) treatment was performed for 2 days to achieve knockdown of endogenous SMARCAD1 before ChIP was carried out. E14 chromatin cross-linking was performed with 2 mM DSG (disuccinimidyl glutarate, Thermo Scientific) in PBS for 45 min at room temperature, washed three times with PBS, and formaldehyde (Polysciences) was added to a final concentration of 1 % for 10 min at room temperature. Formaldehyde was quenched using glycine (0.125 M). Chromatin was eluted in 1% SDS, 100mM NaHCO3 and DNA purified by Qiaquick PCR cleanup columns (Qiagen). Six (FLAG) or four (KAP1) individual ChIPs were pooled and purifed on QIAquick columns (Qiagen). Five nanograms of precipitated DNA were used for library preparation using the Microplex library preparation kit v2 (Diagenode). Libraries were purified on AMPure magnetic beads (Beckman Coulter).

characteristic: description

text: normal

characteristic: disease

text: shSMARCAD1; SMARCAD1-(K523R)

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: SMARCAD1 mutation in ATP binding pocket

text: Mus musculus


characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: normal

text: shSMARCAD1; SMARCAD1-3xFLAG

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

characteristic: phenotype

text: Mus musculus


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: normal

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: wild type phenotype

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: common name

text: normal

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: SMARCAD1 knockdown by shRNA

text: Mus musculus


text: IWGSC

text: public

text: EG PLANTS

characteristic: broker name

text: bread wheat

text: Chinese Spring

characteristic: cultivar

text: Triticum aestivum

text: Triticum aestivum

text: Chinese Spring

characteristic: strain

text: Bread wheat variety Chinese Spring for generating IWGSC RefSeq v1.0 assembly


characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: Approximately 5x10e7 PGK12.1XX (CVCL_AS07) cells per 15 cm plate were cross-linked with 2 mM DSG (disuccinimidyl glutarate, Thermo Scientific) in PBS for 45 min at room temperature, washed three times with PBS, and formaldehyde (Polysciences) was added to a final concentration of 1 % for 10 min at room temperature. Formaldehyde was quenched using glycine (0.125 M). Antibodies used were SMARCAD1 PAB15737 (Abnova) and H3K9me3 ab8898 (abcam). Mouse PGK12.1 ES cells were cultivated feeder-cell free on gelatin-coated dishes in Dulbecco's Modified Eagle Medium (DMEM, Life Technologies) supplemented with 15% foetal calf serum, 2 mM L-glutamine, 1x non-essential amino acids, 50 μM 2-mercaptoethanol, and 1000U/ml ESGRO leukemia inhibitory factor. Generation of SMARCAD1 and control knockdown ES cells was achieved by transfecting shRNA vectors targeting exon 7 of Smarcad1 (shSMARCAD1) or a linker sequence (shControl) as control into PGK12.1 XX cells using Lipofectamine 2000 (Life Technologies), followed by puromycin selection and clonal expansion. Chromatin was eluted in 1% SDS, 100mM NaHCO3 and DNA purified by Qiaquick PCR cleanup columns (Qiagen). Five individual SMARCAD1 ChIPs were pooled and purifed on QIAquick columns (Qiagen). Four nanograms of SMARCAD1 and H3K9me3 antibody precipitated DNA were used for library preparation with the Microplex library preparation kit v2 (Diagenode). Libraries were purified on AMPure magnetic beads (Beckman Coulter).

characteristic: description

text: normal

characteristic: disease

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: PGK x 129P2/Ola/Hsd


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:EMBL - EBI & Wellcome Trust Sanger Institute

unit: year

characteristic: age

text: ArrayExpress

text: fibroblast of dermis

text: human

text: Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 0,2 or 6 hours before collection. Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman).

text: normal

text: wild type genotype

characteristic: genotype

text: bima

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: dermis

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL - EBI & Wellcome Trust Sanger Institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fibroblast of dermis

text: human

characteristic: common name

text: normal

text: wild type genotype

text: bima

text: Homo sapiens

text: Homo sapiens

text: dermis

text: Homo sapiens

text: male

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: fibroblast of dermis

characteristic: cell type

text: human

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

text: bubh

text: Homo sapiens

text: Homo sapiens

text: dermis

text: Homo sapiens

text: female


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

text: fibroblast of dermis

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text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: age

text: ArrayExpress

text: fibroblast of dermis

text: human

text: normal

text: wild type genotype

characteristic: genotype

text: xojn

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: dermis

text: Homo sapiens

text: female