characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGAGACGCGC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

unit: g

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

unit: m

characteristic: depth

text: Medium term abandoned grassland (16-23 years)

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

text: mimarks-survey

text: CATAGTGACA

text: soil metagenome

characteristic: organism

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3.0min; 35 cycles of denaturation: 94degC_1.0min; annealing: 52degC_1.0min;elongation: 72degC_1.0min;final elongation:72degC_5.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.

text: FR1 :5-ANCCATTCAATCGGTANT-3 ; FF390 :5-CGATAACGAACGAGACCT-3

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

unit: % sand/silt/clay

characteristic: title

unit: g/kg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Short term abandoned grassland (6-9 years)

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Podzols

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTGCTGTGT

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Podzols

text: Netherlands

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCTGTCG

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: cork harvest

characteristic: current land use

text: forest

characteristic: current vegetation

characteristic: depth

text: Woodland

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGAGACGCGC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: cork harvest

text: forest

text: Woodland

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTCTAGTAC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: cork harvest

characteristic: current land use

text: forest

characteristic: current vegetation

characteristic: depth

text: Woodland

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGACACTATC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: mixed forest-grassland

text: Wooded pasture grazed and periodically (5-10 years) cultivated to grow annual forage crops

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGACACTATC

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture + annual forage crops

characteristic: current land use

text: mixed forest-grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGACGTGACT

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: mixed forest-grassland

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCTGTCG

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture + annual forage crops

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Intensive grassland grazed and periodically (1-5 years) cultivated to grow annual forage crops

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTCTAGTAC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture + annual forage crops

text: grassland

text: temperate woodland

text: field soil

text: Cambisols

text: Italy

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCGTATA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture + annual forage crops

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate woodland

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Cambisols

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTCGATCTC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CAGTAGACGT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Natural grassland grazed

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CACGTGTCGC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: low

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGACGTGACT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Natural grassland grazed and fertilized

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CATCGAGCAG

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: medium

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: Natural grassland grazed and fertilized

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CAGTAGACGT

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

text: Cultivated grassland (tillage + seeding), grazed and fertilized

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGTCGATCTC

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

text: Pasture

text: grassland

text: temperate grasslands

text: field soil

text: Stagnosols

text: United Kingdom

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCGTATA

characteristic: multiplex identifiers

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

text: yes

characteristic: pooling of DNA extracts (ifdone)

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: 18S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: high

characteristic: Level of intensification of land-use

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Pasture

characteristic: current land use

text: grassland

characteristic: current vegetation

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: Stagnosols

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: mimarks-survey

text: CGCGACATCT

text: soil metagenome

characteristic: organism

text: yes

text: soil metagenome

text: pyrosequencing

text: soil

text: frozen

text: 18S rRNA gene

characteristic: title


text: 34414415; 34461978; 35780211

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CTB/NICD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ZAF

text: NDM Oxford

characteristic: broker name

text: Mycobacterium

characteristic: genus

characteristic: isolate

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0114583_S12. CTB/NICD. TRL0114583_S12

type: AMR curated data - CABBAGE

value: ethambutol

value: resistant

value: Mycobacterium tuberculosis

value: rifampin


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

characteristic: SRA accession

characteristic: Submitter Id

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0114455_S23. CTB/NICD. TRL0114455_S23


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABR00077729_S24. CTB/NICD. BR00077729_S24

value: pyrazinamide

value: susceptible

value: isoniazid


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SAEF00234899_S31. CTB/NICD. EF00234899_S31

value: ofloxacin

value: streptomycin

value: kanamycin


text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0115469_S6. CTB/NICD. TRL0115469_S6


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABH00815045_S6. CTB/NICD. BH00815045_S6


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SACG01628752_S1. CTB/NICD. CG01628752_S1


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SATRL0115095_S12. CTB/NICD. TRL0115095_S12


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABK00078859_S16. CTB/NICD. BK00078859_S16


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABF01506399_S20. CTB/NICD. BF01506399_S20


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABK00082596_S22. CTB/NICD. BK00082596_S22


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABF01375032_S27. CTB/NICD. BF01375032_S27


text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABN00034572_S2. CTB/NICD. BN00034572_S2


text: 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SAND00124877_S3. CTB/NICD. ND00124877_S3


text: 33347430; 34414415; 34461978; 35780211

text: CTB/NICD

text: public

text: ZAF

text: NDM Oxford

text: Mycobacterium

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: SABE01369677_S11. CTB/NICD. BE01369677_S11


text: UC SANTA CRUZ

text: public

text: New England Biolobs Cat. No. N4018S

characteristic: Other Source

tag: Namespace:UC SANTA CRUZ

text: M13 Replicate 1

characteristic: description

text: RF

characteristic: dev stage

text: E. coli ER2738

characteristic: lab host

text: Phage M13mp18

text: Phage M13mp18

characteristic: sub strain

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UC SANTA CRUZ

text: public

text: New England Biolobs Cat. No. N4018S

text: M13 Replicate 2

text: RF

text: E. coli ER2738

text: Phage M13mp18

characteristic: organism

text: Phage M13mp18

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UC SANTA CRUZ

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: New England Biolobs Cat. No. N4018S

text: M13 Replicate 3

text: RF

text: E. coli ER2738

text: Phage M13mp18

text: Phage M13mp18


text: INRA-CMSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Forest

characteristic: collection date

text: Foret

characteristic: current land use

characteristic: depth

text: soil sample under the fallowing soil characteristics (land-use, environmant, geographical coordinates, provided on this datasheet)

text: temperate woodland biome

characteristic: environment (biome)

text: national forest

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTATATGCT

characteristic: multiplex identifiers

text: DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012). Briefly, in a 15 ml Falcon tube 1 g of each soil sample was mixed with 4 ml of a solution containing 100 mM Tris (pH 8.0), 100 mM EDTA (pH 8.0), 100 mM NaCl, and 2% (wt/vol) sodium dodecylsulphate. 2 g of 100 µm diameter silica beads, 2.5 g of 1.4 mm diameter ceramic beads and 4 glass beads of 4 mm diameter were added to the mixture. The samples were then homogenized for 3 x 30 s at 4 m sec-1 in a FastPrep®-24 (MP-Biomedicals, NY, USA) and incubated for 30 min at 70°C before centrifugation at 7000 x g for 5 min at 20°C. For the deproteination, 1 ml of the collected supernatants was incubated for 10 min on ice with 1/10 volume of 3 M potassium acetate (pH 5.5) and centrifuged at 14000 x g for 5 min. Finally, after precipitation with one volume of ice-cold isopropanol, the nucleic acids were washed with 70% ethanol. For purification, DNA extracts were loaded onto PVPP (polyvinyl polypyrrolidone) minicolumns (BIORAD, Marne la Coquette, France) and centrifuged at 1000 x g for 2 min at 10°C. The collected eluate was then purified with the Geneclean Turbo (Q Biogene®, IllKirch, France).

characteristic: nucleic acid extraction

text: soil metagenome

text: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_2.0min; 35 cycles of denaturation: 94degC_0.5min;annealing: 52degC_0.5min;elongation: 72degC_1.0min;final elongation: 72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.

characteristic: pcr conditions

text: F479:5-CAGCMGCYGCNGTAANAC-3; R888:5-CCGYCAATTCMTTTRAGT-3

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Forest

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Foret

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate woodland biome

characteristic: environment (biome)

text: national forest

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGAGCTAGCT

text: soil metagenome

characteristic: organism

text: 5ng of DNA were used for a 25�l PCR conducted under the following conditions: initial denaturation: 94degC_2.0min; 35 cycles of denaturation: 94degC_0.5min;annealing: 52degC_0.5min;elongation: 72degC_1.0min;final elongation: 72degC_7.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Min. Tillage

text: Culture

text: temperate grasslands

text: national forest

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGATCGTATA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Min. Tillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CATCGAGCAG

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Min. Tillage

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTCGATCTC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Catch Crop

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CATATCTCGT

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Catch Crop

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CAGTACTGCG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Catch Crop

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGAGACGCGC

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Catch Crop

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ConventionalTillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGTATGCGAC

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ConventionalTillage

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGATCGTATA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Min. Tillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGCTATCTAT

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Forest

text: Foret

text: temperate woodland biome

text: national forest

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGAGCTCATG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ConventionalTillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGTGAGCTGA

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Forest

text: Foret

text: temperate woodland biome

text: national forest

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CACGTAGATC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Min. Tillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CAGTAGACGT

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ConventionalTillage

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGTACAGTCA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

characteristic: sample weight for DNA extraction

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: freeze-dried

characteristic: storage conditions (fresh/frozen/other)

text: 16S rRNA gene

characteristic: target gene

characteristic: texture

characteristic: total n

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Min. Tillage

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Culture

characteristic: current land use

characteristic: depth

characteristic: description

text: temperate grasslands

characteristic: environment (biome)

text: field soil

characteristic: environment (feature)

text: calcisol

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: metagenome

text: CGTAGACTAG

text: soil metagenome

characteristic: organism

text: soil metagenome

text: pyrosequencing

text: soil

text: freeze-dried

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Min. Tillage

text: Culture

text: temperate grasslands

text: field soil

text: calcisol

text: France

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CAGTACTGCG

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: ph

characteristic: project name

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