text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 14; first primary tumor sample (TU1)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:ETH ZURICH D-BSSE

characteristic: Submitter Id

text: human

characteristic: common name

text: Patient 14; second primary tumor sample (TU2)

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3

characteristic: title


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 15; matched normal sample (NO)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 15; first primary tumor sample (TU1)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 15; second primary tumor sample (TU2)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 16; matched normal sample (NO)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 16; first primary tumor sample (TU1)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: ETH ZURICH D-BSSE

text: public

text: human

text: Patient 16; second primary tumor sample (TU2)

text: Homo sapiens

text: Homo sapiens

text: Illumina HiSeq 2000

text: TruSeq SBS Kit v3


text: P&G Singapore Innovation Center

text: public

tag: Namespace:P&G Singapore Innovation Center

characteristic: collection date

text: Human-associated habitat

characteristic: environment (biome)

text: Malodor aspect

characteristic: environment (feature)

text: skin

characteristic: environment (material)

text: Philippines

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-skin

characteristic: human skin environmental package

text: metagenome

characteristic: investigation type

text: skin metagenome

text: Understanding the microbial basis of body odor in teenagers and kids

characteristic: project name

text: skin metagenome

text: Illumina HiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Technical University of Munich

text: MAP meat

text: MAP meat

text: meat

text: Germany

text: metatranscriptome

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: Photobacterium phosphoreum

text: Metatranscriptome poultry meat

text: Photobacterium phosphoreum

text: Illumina


text: public

tag: Namespace:Institute of Botany, Polish Academy of Sciences

characteristic: Submitter Id

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Spain

characteristic: geographic location (country and/or sea)

text: Delta del Llobregat, Barcelona, Catalunya

characteristic: geographic location (region and locality)

characteristic: lat lon

text: Vanessa cardui

characteristic: organism

text: Vanessa cardui

characteristic: specimen voucher

text: Pollen collected form the insect body

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institute of Botany, Polish Academy of Sciences

text: public

text: Yes

text: Spain

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Yes

text: Spain

text: Maro, M�laga, Andaluc�a

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Yes

text: Spain

text: Venta del Peral, Almer�a, Andaluc�a

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


text: public

characteristic: Submitter Id

characteristic: collection date

text: Yes

text: Spain

text: Moj�car, Almer�a, Andaluc�a

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


text: public

text: Yes

characteristic: environmental sample

text: Spain

characteristic: geographic location (country and/or sea)

text: B�dar, Almer�a, Andaluc�a

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


text: public

text: Yes

text: Spain

text: Frigiliana, M�laga, Andaluc�a

characteristic: geographic location (region and locality)

characteristic: lat lon

text: Vanessa cardui

text: Vanessa cardui

text: Pollen collected form the insect body


text: public

text: Yes

text: Spain

text: Illa Grossa, Illes Columbretes, Castell�

text: Vanessa cardui

characteristic: organism

text: Vanessa cardui

characteristic: specimen voucher

text: Pollen collected form the insect body


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:EUROPEAN MOLECUALR BIOLOGY LABORATORY, GENOME BIOLOGY UNIT

characteristic: cell line

text: human

characteristic: common name

text: Protocols : Growth Protocol: Media was decanted and cells washed with 50 ml ice-cold PBS. In order to remove as much liquid as possible dishes were propped up straight and residual PBS drained onto a paper towel through gravity. UV- crosslinking occurred on ice with 200 mJ/cm2 at 254 nm wavelength with a BIO-LINK UV-crosslinker (Vilber). Cells that had incorporated 4SU were UV-crosslinked at 365 nm wavelength. Subsequently, cells were harvested into ice-cold PBS, pelleted and stored at -80 C for up to 14 days.

characteristic: description

text: DMEM

characteristic: growth media

text: Homo sapiens

text: Homo sapiens

characteristic: sampletype

text: Homo sapiens

characteristic: title

characteristic: treatment type


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EUROPEAN MOLECUALR BIOLOGY LABORATORY, GENOME BIOLOGY UNIT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: DMEM

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: no UV


text: public

characteristic: Submitter Id

text: human

text: DMEM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

characteristic: cell line

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: public

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: no UV


text: public

text: LAMIN-B1 12987-1-AP

characteristic: antibody

text: proteintech

characteristic: antibody source

text: purified polyclonal

characteristic: antibody type

text: human

text: Protocols : Growth Protocol: Cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) for SILAC (Silantes) supplemented with 10% dialysed FBS and Pen-Strep (100 U/ml penicillin, 100 mg/ml streptomycin) at 37C, 5 % CO2. DMEM for SILAC was supplemented with 1 mM L-lysine and 0.5 mM L-arginine of the individual SILAC labels as well as 1.7 mM light L-proline and 1 x GlutaMAX. Cells were grown to 80 % confluence before UV crosslinking.

characteristic: epitope

text: Homo sapiens

text: Homo sapiens

characteristic: sampletype

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: EXOSC2 14805-1-AP

text: proteintech

text: purified polyclonal

text: human

text: Protocols : Growth Protocol: Sodium Arsenite was added at 400 microM concentration from an aqueous stock solution of 0.05 M.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: 0 minutes

characteristic: treatment time


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: EXOSC2 14805-1-AP

text: proteintech

text: purified polyclonal

characteristic: cell line

text: human

text: Arsenite

characteristic: compound

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: 5 minutes


text: public

text: EXOSC2 14805-1-AP

characteristic: antibody

text: proteintech

characteristic: antibody source

text: purified polyclonal

characteristic: antibody type

text: human

characteristic: common name

text: Arsenite

characteristic: description

characteristic: epitope

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: 30 minutes


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: human

text: Arsenite

text: Homo sapiens

text: Homo sapiens

characteristic: sampletype

text: Homo sapiens

characteristic: title

text: 30 minutes


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: 0 minutes

characteristic: treatment time


text: public

characteristic: Submitter Id

characteristic: cell line

text: human

text: Arsenite

characteristic: compound

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: 5 minutes


text: public

tag: Namespace:Institute of Molecular Oncology, Philipps-University Marburg

text: ArrayExpress

characteristic: broker name

text: mouse embryonic fibroblast cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Primary mouse embryonic fibroblasts (MEFs) expressing wild type p53 where isolated from three mouse embryos. MEFs expressing mutant p53(Arg178Asp) (so-called EE mutant of p53) where isolated from four embryos and three embryos led to the isolation of three cell preparations that do not express any p53 (p53 KO). The MEFs were kept in culture as triplicates (13x3=39 samples)until they reached near confluence. Primary MEFs were isolated from E12-14.5 mouse embryos using standard protocols and amplified at low oxygen conditions (5% O2). Primary MEFs were treated for 16 hours with 10 µM nutlin-3a or 0,1% DMSO as control. Cells were fixed on plates with 0.88% paraformaldehyde (PFA) for 10 min at room temperature and quenched by adding glycine to a final concentration of 54 mM for 5 min. Cells were washed twice with ice-cold PBS and scraped off the plate with PBS supplemented with proteinase-inhibitors (Roche). Cell pellets were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.1) supplemented with protease-inhibitors (2x107 cells/ml lysis buffer) and sonicated using the X Bioruptor® Sonication System (Diagenode) to obtain 300-1000 bp DNA fragments. The shearing efficiency was controlled using agarose gel electrophoresis. After centrifugation (10000 g, 10 min, 20°C), 100 µl sheared chromatin was diluted 1:10 with dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl pH 8.1, 167 mM NaCl) and pre-cleared for 1 h with 50 µl Protein G sepharose (50% slurry in 20% ethanol) beads (GE Healthcare) at 4°C. The p53 protein was immunoprecipitated overnight at 4°C with 2.5 µg anti-p53 antibody (FL393, Santa Cruz), normal rabbit IgG (Santa Cruz) was used as the control. The protein complexes were pulled down for 4 hours at 4°C with 50 µl Protein G sepharose beads. Beads were washed with Low Salt Immune Complex Wash Buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.1, 150 mM NaCl), then with High Salt Immune Complex Wash Buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.1, 500 mM NaCl), with LiCl Immune Complex Wash Buffer (0.25 M LiCl, 1% IGEPAL-CA630, 1% deoxycholic acid (sodium salt), 2 mM EDTA, 10 mM Tris-HCl pH 8.1), and finally twice with TE (10 mM Tris-HCl pH 8.1, 1 mM EDTA). Protein/DNA crosslinks were eluted twice for 15 min at 20°C in 100 µl elution buffer (0.1 M NaHCO3, 1% SDS). 1% input stored at -20°C was treated in the same way. Crosslinking was reverted upon overnight incubation at 65°C in elution buffer supplemented with 200 mM NaCl followed by RNaseA digestion at 37°C for 30 min, addition of 40 mM Tris-HCl pH 6.5 and 10 mM EDTA, proteinase K digestion for 2 hours at 55°C and inactivation of proteinase K at 99°C for 10 min. DNA was purified using the QIAquick PCR Purification Kit (Qiagen). ChIP-seq libraries were prepared from purified ChIP DNA with the Microplex Library Preparation Kit (Diagenode) according to the manufacturer’s instructions.

characteristic: description

text: embryonic day 12.5 to 14.5

characteristic: developmental stage

text: Tg(Prm-cre)58Og/J; Trp53 -/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: embryo

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Molecular Oncology, Philipps-University Marburg

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: mouse embryonic fibroblast cell

text: house mouse

text: embryonic day 12.5 to 14.5

text: Tg(Prm-cre)58Og/J; Trp53 -/-

text: Mus musculus

text: Mus musculus

text: embryo

text: Mus musculus

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: mouse embryonic fibroblast cell

text: house mouse

text: embryonic day 12.5 to 14.5

text: Tg(Prm-cre)58Og/J; Trp53 Arg178Asp

text: Mus musculus

text: Mus musculus

text: embryo

text: Mus musculus


text: public

text: ArrayExpress

characteristic: broker name

text: mouse embryonic fibroblast cell

characteristic: cell type

text: house mouse

characteristic: common name

text: embryonic day 12.5 to 14.5

text: Tg(Prm-cre)58Og/J; wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: embryo

text: Mus musculus


text: public

text: ArrayExpress

text: mouse embryonic fibroblast cell

text: house mouse

text: Protocols: Primary mouse embryonic fibroblasts (MEFs) expressing wild type p53 where isolated from three mouse embryos. MEFs expressing mutant p53(Arg178Asp) (so-called EE mutant of p53) where isolated from four embryos and three embryos led to the isolation of three cell preparations that do not express any p53 (p53 KO). The MEFs were kept in culture as triplicates (13x3=39 samples) until they reached near confluence. Primary MEFs were isolated from E12-14.5 mouse embryos using standard protocols and amplified at low oxygen conditions (5% O2). MEFs were treated for 6.5 hours with either 10µM Nutlin3a in DMSO or DMSO (10µl) alone. Total RNAs were purified using the RNeasy kit and the Qiacube robot from Qiagen. The quality of RNAs and the p53-specific induction of p21 expression upon Nutlin3a treatment has been verified by Reverse Transcription - quantitative Polymerase Chain Reaction (RTqPCR) using the Superscript VILO cDNA synthesis kit from Invitrogen and the Absolute qPCR cybergreen mix from Thermo Scientific, respectively. qPCR was performed on the Lightcycler 480 from Roche. RNASeq libraries were prepared from total RNA using the TruSeq Stranded mRNA LT kit (Illumina) according to the manufacturer’s instructions.

characteristic: description

text: embryonic day 12.5 to 14.5

characteristic: developmental stage

text: Tg(Prm-cre)58Og/J; Trp53 Arg178Asp

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: embryo

characteristic: organism part

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: mouse embryonic fibroblast cell

text: house mouse

text: embryonic day 12.5 to 14.5

text: Tg(Prm-cre)58Og/J; Trp53 -/-

text: Mus musculus

text: Mus musculus

text: embryo

text: Mus musculus

characteristic: strain


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: mouse embryonic fibroblast cell

text: house mouse

text: embryonic day 12.5 to 14.5

text: Tg(Prm-cre)58Og/J; wild type genotype

text: Mus musculus

text: Mus musculus

text: embryo

text: Mus musculus

characteristic: title


text: CBMSO

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Balb/c mice

characteristic: lab host

text: Mus musculus

text: Mus musculus

text: female

characteristic: sex

text: Cervical Lymph nodes

characteristic: tissue type

text: Mock LN A


text: CBMSO

text: public

text: house mouse

characteristic: common name

text: Balb/c mice

text: Mus musculus

characteristic: organism

text: Mus musculus

text: female

text: Cervical Lymph node

text: Mock LN B


characteristic: ENA-CHECKLIST

text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: Mock LG A


characteristic: ENA-FIRST-PUBLIC

text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: Mock LGB


characteristic: ENA-LAST-UPDATE

text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Spleen

text: Mock SP A


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Spleen

text: Mock SP B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph nodes

text: VACV WR 5dpi LN A


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph node

text: VACV WR 5dpi LN B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: VACV WR 5dpi LG B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Spleen

text: VACV WR 5dpi SP B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph nodes

text: VACV dIFNBP 5dpi LN A


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph node

text: VACV dIFNBP 5dpi LN B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: VACV dIFINBP 5dpi LG B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: VACV dIFNBP 5dpi LG B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Spleen

text: VACV dIFNBP 5dpi SP B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph nodes

text: VACV IFNBP_GAGmut 5dpi LN A


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Cervical Lymph node

text: VACV IFNBP_GAGmut 5dpi LN B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Lung

text: VACV IFNBP_GAGmut 5dpi LG B


text: CBMSO

text: public

text: house mouse

text: Balb/c mice

text: Mus musculus

text: Mus musculus

text: female

text: Spleen

text: VACV IFNBP_GAGmut 5dpi SP B


text: UNIVERSITY OF SZEGED

text: public

tag: Namespace:UNIVERSITY OF SZEGED

text: Mixed Infection

characteristic: description

text: Vaccinia virus

text: Vaccinia virus


characteristic: External Id

text: UNIVERSITY OF SZEGED

text: public

text: Vaccinia_virus_LT966077_GMAP_CAP_VACV_MinIon.sorted.bam

text: Mixed Infection

text: Vaccinia virus

text: Vaccinia virus


text: UNIVERSITY OF SZEGED

characteristic: INSDC center name

text: public

text: Vaccinia_virus_LT966077_GMAP_cDNA_VACV_MinIon.sorted.bam

text: Mixed Infection

text: Vaccinia virus

text: Vaccinia virus


status: KILLED

text: Public Health Agency of Sweden

characteristic: INSDC first public

text: killed

text: sam_FOHMtest_d211c1f4-021c-4eb9-81fa-8e3f3de7e5fe

tag: Namespace:Public Health Agency of Sweden

text: FOHM

text: Sweden

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

text: yes

characteristic: isolate

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Department of Metabolic Disorder, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Male C57BL/6JJcl mice at the age of 8 weeks were purchased, and five animals per cage were fed a chow diet initially. After 2-week of acclimation, half the mice were randomly assigned to switch from a standard chow to a high-fat diet (D12492, 60% fat, 20% carbohydrate, 20% protein, 5.24 kcal/g; Research Diets Inc., USA). Pancreatic islets were isolated 27 weeks later both from experimental and control group. Total RNA was extracted using Trizol (Life Technologies). Five hundred nano grams of total RNA were used as input material for library preparation using the TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc.) according to manufacturer’s instructions. Briefly, after the rRNA depletion using Ribo-Zero Gold technology, the remaining RNA is fragmented by heat and cation treatment, and primed for cDNA synthesis. Synthesis of First Strand cDNA was done using random hexamers and reverse transcriptase in addition to Actinomycin D that allows RNA-dependent synthesis and improved strand specificity. Blunt-ended ds cDNA is generated by incorporating dUTP in place of dTTP and purified using AMPure XP beads. After addition of a single ‘A’ nucleotide to the 3’ ends of the blunt fragments, adapter ligation to the fragment is achieved by a complementary overhanging of ‘T’ nucleotide on the 3’ end. Finally, DNA fragments that have adapter molecules on both ends are amplified by PCR using a Primer Cocktail that anneals to the ends of the adapters.

text: CE-2 (4.6% fat, 69.9% carbohydrate, 25.5% protein, 3.39 kcal/g; CLEA Japan Inc., Tokyo)

characteristic: diet

text: normal

characteristic: disease

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: normal glucose tolerance

characteristic: phenotype

text: Mus musculus

text: male

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Metabolic Disorder, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine

text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: D12492 (60% fat, 20% carbohydrate, 20% protein, 5.24 kcal/g; Research Diets Inc., USA)

text: obesity

text: Mus musculus

characteristic: organism

text: Mus musculus

text: islet of Langerhans

text: mild glucose intolerance

text: Mus musculus

text: male

characteristic: sex


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Male C57BL/6JJcl mice at the age of 8 weeks were purchased, and five animals per cage were fed a chow diet initially. After 2-week of acclimation, half the mice were randomly assigned to switch from a standard chow to a high-fat diet (D12492, 60% fat, 20% carbohydrate, 20% protein, 5.24 kcal/g; Research Diets Inc., USA). Pancreatic islets were isolated 27 weeks later both from experimental and control group. Nucleic acid extraction was done using Agencourt AMPure XP beads according to the instruction of NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England BioLabs Inc.). ChIP libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England BioLabs Inc.). The DNA precipitated by ChIP was further fragmented to an average size of ∼200 bp by using S2 Focused-ultrasonicator (Covaris), before library construction. By 18 cycles of PCR amplification from 7.8 ng of fragmented ChIP samples, sequencing libraries were generated.

text: normal

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

text: normal glucose tolerance

text: Mus musculus

text: male


text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: diet

text: obesity

characteristic: disease

text: Mus musculus

text: Mus musculus

text: islet of Langerhans

characteristic: organism part

text: mild glucose intolerance

characteristic: phenotype

text: Mus musculus

text: male

characteristic: strain


G DS 1618

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COST Action

text: public

text: G DS 1618

tag: Namespace:COST Action

characteristic: Submitter Id

text: gut metagenome

text: gut metagenome

text: G DS 1618

characteristic: title


G DS 1619

text: COST Action

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: G DS 1619

text: gut metagenome

characteristic: organism

text: gut metagenome

text: G DS 1619


G DS 1622

text: COST Action

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: G DS 1622

text: gut metagenome

text: gut metagenome

text: G DS 1622


G DS 1623

text: COST Action

text: public

text: G DS 1623

text: gut metagenome

text: gut metagenome

text: G DS 1623


G DS 1571

text: COST Action

text: public

text: G DS 1571

text: gut metagenome

text: gut metagenome

text: G DS 1571


G DS 1572

text: COST Action

text: public

text: G DS 1572

text: gut metagenome

text: gut metagenome

text: G DS 1572


G DS 1516

text: COST Action

text: public

text: G DS 1516

text: gut metagenome

text: gut metagenome

text: G DS 1516


G DS 1517

text: COST Action

text: public

text: G DS 1517

text: gut metagenome

text: gut metagenome

text: G DS 1517


G DS 1518

text: COST Action

text: public

text: G DS 1518

text: gut metagenome

text: gut metagenome

text: G DS 1518


G DS 1455

text: COST Action

text: public

text: G DS 1455

text: gut metagenome

text: gut metagenome

text: G DS 1455


G RS 1620

text: COST Action

text: public

text: G RS 1620

text: gut metagenome

text: gut metagenome

text: G RS 1620


G RS 1621

text: COST Action

text: public

text: G RS 1621

text: gut metagenome

text: gut metagenome

text: G RS 1621


G RS 1624

text: COST Action

text: public

text: G RS 1624

text: gut metagenome

text: gut metagenome

text: G RS 1624


G RS 1625

text: COST Action

text: public

text: G RS 1625

text: gut metagenome

text: gut metagenome

text: G RS 1625


G RS 1570

text: COST Action

text: public

text: G RS 1570

text: gut metagenome

text: gut metagenome

text: G RS 1570


G RS 1569

text: COST Action

text: public

text: G RS 1569

text: gut metagenome

text: gut metagenome

text: G RS 1569


G RS 1514

text: COST Action

text: public

text: G RS 1514

text: gut metagenome

text: gut metagenome

text: G RS 1514


G RS 1515

text: COST Action

text: public

text: G RS 1515

text: gut metagenome

text: gut metagenome

text: G RS 1515


G RS 1521

text: COST Action

text: public

text: G RS 1521

text: gut metagenome

text: gut metagenome

text: G RS 1521


G RS 1457

text: COST Action

text: public

text: G RS 1457

text: gut metagenome

text: gut metagenome

text: G RS 1457


L DS 1618

text: COST Action

text: public

text: L DS 1618

text: gut metagenome

text: gut metagenome

text: L DS 1618


L DS 1619

text: COST Action

text: public

text: L DS 1619

text: gut metagenome

text: gut metagenome

text: L DS 1619


L DS 1622

text: COST Action

text: public

text: L DS 1622

text: gut metagenome

text: gut metagenome

text: L DS 1622


L DS 1623

text: COST Action

text: public

text: L DS 1623

text: gut metagenome

text: gut metagenome

text: L DS 1623


L DS 1571

text: COST Action

text: public

text: L DS 1571

text: gut metagenome

text: gut metagenome

text: L DS 1571


L DS 1572

text: COST Action

text: public

text: L DS 1572

text: gut metagenome

text: gut metagenome

text: L DS 1572


L DS 1516

text: COST Action

text: public

text: L DS 1516

text: gut metagenome

text: gut metagenome

text: L DS 1516


L DS 1517

text: COST Action

text: public

text: L DS 1517

text: gut metagenome

text: gut metagenome

text: L DS 1517


L DS 1518

text: COST Action

text: public

text: L DS 1518

text: gut metagenome

text: gut metagenome

text: L DS 1518


L DS 1455

text: COST Action

text: public

text: L DS 1455

text: gut metagenome

text: gut metagenome

text: L DS 1455


L RS 1620

text: COST Action

text: public

text: L RS 1620

text: gut metagenome

text: gut metagenome

text: L RS 1620


L RS 1621

text: COST Action

text: public

text: L RS 1621

text: gut metagenome

text: gut metagenome

text: L RS 1621


L RS 1624

text: COST Action

text: public

text: L RS 1624

text: gut metagenome

text: gut metagenome

text: L RS 1624


L RS 1625

text: COST Action

text: public

text: L RS 1625

text: gut metagenome

text: gut metagenome

text: L RS 1625


L RS 1570

text: COST Action

text: public

text: L RS 1570

text: gut metagenome

text: gut metagenome

text: L RS 1570


L RS 1569

text: COST Action

text: public

text: L RS 1569

text: gut metagenome

text: gut metagenome

text: L RS 1569


L RS 1514

text: COST Action

text: public

text: L RS 1514

text: gut metagenome

text: gut metagenome

text: L RS 1514


L RS 1515

text: COST Action

text: public

text: L RS 1515

text: gut metagenome

text: gut metagenome

text: L RS 1515


L RS 1521

text: COST Action

text: public

text: L RS 1521

text: gut metagenome

text: gut metagenome

text: L RS 1521


L RS 1457

text: COST Action

text: public

text: L RS 1457

text: gut metagenome

text: gut metagenome

text: L RS 1457


43973 DS

text: COST Action

text: public

text: 43973 DS

text: gut metagenome

text: gut metagenome

text: 43973 DS


43981 DS

text: COST Action

text: public

text: 43981 DS

text: gut metagenome

text: gut metagenome

text: 43981 DS


43942 DS

text: COST Action

text: public

text: 43942 DS

text: gut metagenome

text: gut metagenome

text: 43942 DS


43916 DS

text: COST Action

text: public

text: 43916 DS

text: gut metagenome

text: gut metagenome

text: 43916 DS


44097 RS

text: COST Action

text: public

text: 44097 RS

text: gut metagenome

text: gut metagenome

text: 44097 RS


44110 RS

text: COST Action

text: public

text: 44110 RS

text: gut metagenome

text: gut metagenome

text: 44110 RS


44053 RS

text: COST Action

text: public

text: 44053 RS

text: gut metagenome

text: gut metagenome

text: 44053 RS


44081 RS

text: COST Action

text: public

text: 44081 RS

text: gut metagenome

text: gut metagenome

text: 44081 RS


43956 DS

text: COST Action

text: public

text: 43956 DS

text: gut metagenome

text: gut metagenome

text: 43956 DS


43996 DS

text: COST Action

text: public

text: 43996 DS

text: gut metagenome

text: gut metagenome

text: 43996 DS


43873 DS

text: COST Action

text: public

text: 43873 DS

text: gut metagenome

text: gut metagenome

text: 43873 DS


44004 RS

text: COST Action

text: public

text: 44004 RS

text: gut metagenome

text: gut metagenome

text: 44004 RS


44017 RS

text: COST Action

text: public

text: 44017 RS

text: gut metagenome

text: gut metagenome

text: 44017 RS


43845 RS

text: COST Action

text: public

text: 43845 RS

text: gut metagenome

text: gut metagenome

text: 43845 RS


44065 RS

text: COST Action

text: public

text: 44065 RS

text: gut metagenome

text: gut metagenome

text: 44065 RS


text: Anopheles gambiae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: African malaria mosquito

characteristic: common name

text: Anopheles gambiae

text: Anopheles gambiae

characteristic: subject id


text: normal

characteristic: ArrayExpress-PHENOTYPE

text: Staphylococcus aureus

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: normal

characteristic: phenotype

text: Staphylococcus aureus


text: healthy nasal carrier

text: Staphylococcus aureus

characteristic: ENA-CHECKLIST

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: healthy nasal carrier

text: Staphylococcus aureus


text: Staphylococcus aureus

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: healthy

text: Staphylococcus aureus

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: healthy

text: Staphylococcus aureus


characteristic: External Id

text: public

tag: Namespace:Department of Medical Biochemistry and Cell Biology Institute of Biomedicine, The Sahlgrenska Academy University of Gothenburg

text: ArrayExpress

characteristic: broker name

text: CLB-BAR

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: Protocols: The CLB-BAR, CLB-GE and SK-N-AS cell lines were grown for 1hr and 24hrs in the absence (control conditions) or presence of crizotinib (250nM) or lorlatinib (30nM) Total RNA was isolated using the Promega Total RNA Isolation Kit. Random primed cDNA library preparation has been performed by GATC Biotech (Konstanz, Germany) using proprietary methods.

characteristic: description

text: neuroblastoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens


text: Department of Medical Biochemistry and Cell Biology Institute of Biomedicine, The Sahlgrenska Academy University of Gothenburg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

text: CLB-BAR

text: epithelial cell

text: human

text: neuroblastoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

text: E-MTAB-6654:NG-8102_19_lib72268_3711_7_1

text: ArrayExpress

text: CLB-GE

text: epithelial cell

text: human

characteristic: common name

text: neuroblastoma

text: bone marrow

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6654:NG-8102_23_lib72272_3711_7_1

text: ArrayExpress

characteristic: broker name

text: SK-N-AS

text: neuroblast

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


text: public

text: E-MTAB-6654:NG-8102_29_lib72278_3711_6_1

text: ArrayExpress

text: SK-N-AS

characteristic: cell line

text: neuroblast

characteristic: cell type

text: human

characteristic: description

text: neuroblastoma

characteristic: disease

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6654:NG-8102_10_lib72259_3711_3_1

text: ArrayExpress

text: CLB-BAR

text: epithelial cell

text: human

text: neuroblastoma

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens


text: public

characteristic: INSDC status

text: E-MTAB-6654:NG-8102_11_lib72260_3737_1_1

characteristic: Submitter Id

text: ArrayExpress

text: CLB-GE

text: epithelial cell

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

text: E-MTAB-6654:NG-8102_20_lib72269_3711_2_1

text: ArrayExpress

text: CLB-GE

text: epithelial cell

text: human

characteristic: common name

text: neuroblastoma

text: bone marrow

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6654:NG-8102_21_lib72270_3711_2_1

text: ArrayExpress

characteristic: broker name

text: SK-N-AS

text: neuroblast

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


text: public

text: E-MTAB-6654:NG-8102_30_lib72279_3711_6_1

text: ArrayExpress

text: SK-N-AS

characteristic: cell line

text: neuroblast

characteristic: cell type

text: human

characteristic: description

text: neuroblastoma

characteristic: disease

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6654:NG-8102_12_lib72261_3711_7_1

text: ArrayExpress

text: CLB-GE

text: epithelial cell

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: Homo sapiens


text: public

characteristic: INSDC status

text: E-MTAB-6654:NG-8102_18_lib72267_3711_2_1

characteristic: Submitter Id

text: ArrayExpress

text: CLB-GE

text: epithelial cell

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

text: E-MTAB-6654:NG-8102_22_lib72271_3711_7_1

text: ArrayExpress

text: SK-N-AS

text: neuroblast

text: human

characteristic: common name

text: neuroblastoma

text: bone marrow

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-6654:NG-8102_28_lib72277_3711_8_1

text: ArrayExpress

characteristic: broker name

text: SK-N-AS

text: neuroblast

text: human

text: neuroblastoma

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: Homo sapiens


text: INSTITUTE OF CANCER RESEARCH

text: public

tag: Namespace:INSTITUTE OF CANCER RESEARCH

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Tol2 PAR reporter integration sites


text: UPSC

characteristic: INSDC center name

text: public

text: Norway spruce

text: Needles selected on 2011-05-16 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

characteristic: INSDC first public

text: public

text: Norway spruce

text: Needles selected on 2011-05-16 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

characteristic: INSDC last update

text: public

text: Norway spruce

text: Needles selected on 2011-05-16 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-19 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-19 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-19 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-19 biological replicate 4 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-24 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-24 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-24 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-27 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-27 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-27 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-31 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-31 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-05-31 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: INSTITUTE OF CANCER RESEARCH

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Tol2 PAR reporter integration sites


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-03 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-03 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-03 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-07 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-07 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-07 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-10 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-10 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-10 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-14 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-14 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-14 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-17 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-17 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-17 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-20 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: INSTITUTE OF CANCER RESEARCH

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Tol2 PAR reporter integration sites


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-20 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-20 biological replicate 3 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-23 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-23 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-23 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-27 biological replicate 1 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-27 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-27 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-06-30 biological replicate 1 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-04 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-04 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-04 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-11 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-11 biological replicate 2 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-11 biological replicate 3 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-18 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-18 biological replicate 2 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-18 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-25 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-07-25 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-01 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-01 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-08 biological replicate 1 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-08 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-08 biological replicate 3 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-22 biological replicate 1 techical replicate 4

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-22 biological replicate 2 techical replicate 5

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-08-22 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-06 biological replicate 1 techical replicate 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-06 biological replicate 2 techical replicate 4

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-06 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-20 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-20 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-09-20 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-10-17 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-10-17 biological replicate 2 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-10-17 biological replicate 3 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-11-16 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-11-16 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2011-11-16 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-02-21 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-02-21 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-02-21 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-03-27 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-03-27 biological replicate 2 techical replicate 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-03-27 biological replicate 3 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-04-24 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-04-24 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-05-02 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-05-02 biological replicate 2 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-05-11 biological replicate 1 techical replicate 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Needles selected on 2012-05-11 biological replicate 2 techical replicate 2

text: Picea abies

text: Picea abies


text: Homo sapiens

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: human

text: Homo sapiens

text: Homo sapiens

characteristic: subject id


text: INSTITUT PASTEUR

text: public

characteristic: INSDC status

tag: Namespace:INSTITUT PASTEUR

characteristic: Submitter Id

characteristic: collection date

text: Cambodia

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Escherichia coli

characteristic: isolate

text: Rectal swab

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli

text: E. coli from humans and food in Phnom Penh


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UOM

text: public

characteristic: SRA accession

text: ArrayExpress

characteristic: cell line

text: human

characteristic: common name

text: Protocols: MFD-1 cells were grown in DMEM supplemented with 10% foetal bovine serum and 1% PenStrep. ATAC-seq was performed as published in Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods, 10(12), 1213-1218. http://doi.org/10.1038/nmeth.2688 Nuclei were incubated at 37degC for 30 minutes with Tn5 transposase. DNA was purified using Zymo DNA Clean and Concentrator 5. Libraries were constructed as described in Nature Methods, 10(12), 1213-1218.

text: Oesophageal adenocarcinoma

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: esophagus

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: External Id

text: UOM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: ATAC-seq was performed as described in 'An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues' Nature Methods volume 14, pages 959â€“962 (2017) Nuclei were incubated at 37degC for 30 minutes with Tn5 transposase. DNA was purified using Zymo DNA Clean and Concentrator 5. Libraries were constructed as described in Nature Methods, 10(12), 1213-1218.

characteristic: description

text: Oesophageal adenocarcinoma

text: Homo sapiens

text: Homo sapiens

text: esophagus

text: Homo sapiens


text: Anopheles gambiae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae

characteristic: subject id


characteristic: ENA-LAST-UPDATE

text: Public Health Agency of Sweden

text: killed

characteristic: SRA accession

text: sam_FOHMtest_b32be2ad-5049-41fe-b018-4681e1cefb6e

text: FOHM

text: Sweden

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

text: yes

characteristic: isolate

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


characteristic: External Id

text: public

tag: Namespace:Center for Neuroscience and Cell Biology, University of Coimbra, Portugal

text: ArrayExpress

characteristic: cell line

text: monocyte

characteristic: cell type

text: human

characteristic: common name

text: Protocols: PMA-differentiated THP-1 cells monolayers were infected with R. conorii, R. montanensis at a multiplicity of infection of 10 or maintained uninfected and, at 1 hour post-infection, culture medium was removed and monolayers were washed 1x with PBS and scrapped. PMA-differentiated THP-1 cells monolayers at a cell confluency of 1.2 x 106 cells per well, in 6 well plates, were infected with R. conorii, R. montanensis at a multiplicity of infection of 10 or maintained uninfected. Plates were centrifuged at 300 g for 5 min at room temperature to induce contact between rickettsiae and host cells, and incubated at 34 °C and 5% CO2 for 1 hour. Total RNA was purified using SurePrep True Total RNA purification kit (ThermoFisher Scientific). DNA was removed from the RNA purification using Ambion Turbo DNAse. Removal of DNA contamination was verified by PCR. RNA was re-isolated using PureLink RNA Mini kit (Ambion). After confirmation of RNA structural integrity, 5 μg RNA per sample were subjected to ribosomal RNA depletion using RiboMinusTM Eukaryote System v2 (Ambion) protocols. cDNA libraries were then constructed using the Ion Total RNAseq Kit v2 (Ion torrent).

text: acute monocytic leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

unit: hour

characteristic: time

characteristic: title


characteristic: ENA-CHECKLIST

text: Center for Neuroscience and Cell Biology, University of Coimbra, Portugal

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: monocyte

text: human

characteristic: description

text: acute monocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens


E-MTAB-6723:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-6723:Sample 10

tag: Namespace:Institute of Microbiology and Infection Immunology, Charite - University Medicine Berlin, 12203 Berlin, Germany

text: 8 to 10

characteristic: age

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Protocols: Colonic epithelial cells were dissociated in HBSS containing 5mM EDTA and 10mM HEPES. Cells were further digested using Dispase (5 units per ml; BD Biosciences ), CollagenaseD (0.5 mg/ml; Roche) and DNaseA (0.5 mg/ml; Sigma-Aldrich). Cells were highly purified as DAPI-EpCam+CD45-CD24MedLgr5+ For γ-irradiation of mice, animals were exposed to a Caesium137 source in a OB58/905-2 irradiator (Buchler, Germany). total RNA was isolated using Trizol according to the Immgen protocol (https://www.immgen.org/Protocols/Total%20RNA%20Extraction%20with%20Trizol.pdf) SMARTer Ultra Low Input RNA (Clontech) was used for library preparation

text: Lgr5-Cre-ERT2; Il22-/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: colon

characteristic: phenotype

text: Mus musculus

characteristic: strain

text: Sample 10


E-MTAB-6723:Sample 11

text: Institute of Microbiology and Infection Immunology, Charite - University Medicine Berlin, 12203 Berlin, Germany

text: public

text: E-MTAB-6723:Sample 11

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

characteristic: cell type

text: house mouse

characteristic: common name

text: Lgr5-Cre-ERT2; Il22-/-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: Sample 11

characteristic: title


E-MTAB-6723:Sample 12

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6723:Sample 12

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Lgr5-Cre-ERT2; Il22-/-

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 12


E-MTAB-6723:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-6723:Sample 7

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: epithelial cell of large intestine

text: house mouse

characteristic: description

text: Lgr5-Cre-ERT2; Il22-/-

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 7


E-MTAB-6723:Sample 8

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-6723:Sample 8

text: 8 to 10

characteristic: age

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Lgr5-Cre-ERT2; Il22-/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: colon

characteristic: phenotype

text: Mus musculus

text: Sample 8


E-MTAB-6723:Sample 9

text: public

text: E-MTAB-6723:Sample 9

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

characteristic: cell type

text: house mouse

characteristic: common name

text: Lgr5-Cre-ERT2; Il22-/-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Sample 9


E-MTAB-6723:Sample 1

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6723:Sample 1

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 1

characteristic: title


E-MTAB-6723:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

text: E-MTAB-6723:Sample 2

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: epithelial cell of large intestine

text: house mouse

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 2


E-MTAB-6723:Sample 3

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-6723:Sample 3

text: 8 to 10

characteristic: age

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 3


E-MTAB-6723:Sample 4

text: public

text: E-MTAB-6723:Sample 4

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

characteristic: cell type

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

characteristic: phenotype

text: Mus musculus

text: Sample 4


E-MTAB-6723:Sample 5

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-6723:Sample 5

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

characteristic: strain

text: Sample 5


E-MTAB-6723:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

text: E-MTAB-6723:Sample 6

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: epithelial cell of large intestine

text: house mouse

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: Sample 6

characteristic: title


characteristic: External Id

text: public

characteristic: SRA accession

tag: Namespace:Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-41296, Sweden

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: 10OD of cells were collected, pelleted and snap frozen in liquid nitrogen. The S. cerevisiae strain CEN.PK113-7D was pre-cultured in a batch culture in Delft media with 2% glucose at 30°C and 200 rpm in shake flasks and then transferred to the chemostats in duplicates. Four different media compositions with a limitation in a different nutrient were employed in the chemostat runs. The temperature was also set to 30°C. The dilution rate was fixed to 0.1 and the cells were grown for 4 days to achieve stable cell numbers in the culture. Each environmental stress condition is described as follows: Low Glucose: minimal media with 0.75 % glucose and 1.35 g/l NH4. Anaerobic condition, low Glucose: minimal media with 1 % glucose and 1.35 g/l NH4, continuous gassed with N2. Low Ethanol: minimal media with 0.5 % ethanol and 1.35 g/l NH4. Low Nitrogen: minimal media with 6 % glucose and 0.27 g/l NH4 RNA was extracted using the RNeasy® Mini Kit from QIAGEN. Cells were mechanically disrupted using a FastPrep®-24 from MPbio in combination with the lysing matrix tubes type C from MPbio. The FastPrep was run 3 times for 20 seconds with 4.0m/s settings and a 5 minute break in between each run. RNA quality was assessed using NanoDrop and Bioanalyzer. The Illumina Kit TrueSeq RNA stranded HT kit was used following the manufacturers protocol.

characteristic: description

text: wild type genotype

text: minimal media in chemostat

characteristic: growth condition

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


tag: attribute

text: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-41296, Sweden

text: public

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: wild type genotype

characteristic: genotype

text: minimal media in chemostat

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


E-MTAB-6108:THY1 Negative Cells1

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-6108:THY1 Negative Cells1

tag: Namespace:School of Medicine, Menzies Institute for Medical Research, University of Tasmania, Hobart, Australia

text: ArrayExpress

characteristic: cell line

text: retinal ganglion cell

characteristic: cell type

text: human

text: Protocols: The reporter line BRN3B-mCherry A81-H7 hESC was maintained on vitronectin-coated 6-well plates using StemFlex (Gibco). Cells were differentiated into retinal ganglion cells (RGCs). Briefly, undifferentiated hESCs cultured in monolayer on vitronectin-coated plates were differentiated using RGC differentiation medium 2 (DMEM F12 with GlutaMAX, 10% KnockOut Serum Replacement (Invitrogen), SM1 (STEMCELL), 10 ng/mL noggin (Sapphire Biosciences), 10 ng/ml Dickkopf-related protein 1 (DKK1, Peprotech), 10 ng/ml Insulin Growth Factor (IGF1, Peprotech) and 5 ng/ml basic Fibroblast Growth Factor (bFGF, Merck). Medium was changed every 2-3 days. RGC differentiation was monitored by the appearance of mCherry-positive cells, reflective of BRN3B expression. On day 36 of differentiation, cells were washed with phosphate-buffered saline (PBS, pH 7.4) and incubated with accutase (Sigma) (37°C, 5 minutes). Cells were then incubated in RGC differentiation medium supplemented with the ROCK inhibitor Y27632 (10 μM, Selleckchem, RGC+RI) and gently dissociated using a P1000 pipette, filtered using a 100 μm nylon strainer (BD Falcon) and centrifuged (300g, 10 minutes). The cell pellet was resuspended in RGC+RI medium and incubated with THY1 antibody (Miltenyi, 4°C, 15 minutes). Cells were washed in RGC+RI medium, and centrifuged (300g, 3 minutes). Both THY1-positive (+ve) and THY1-negative (-ve) fractions separated by FACS were subjected to library preparation using the Single Cell 3’ Reagent Kit (10X Genomics) as per the manufacturer’s instruction. Single cell suspensions were loaded onto 10X Genomics Single Cell 3' Chips along with the reverse transcription (RT) master mix as per the manufacturer's protocol for the Chromium Single Cell 3' v2 Library (10X Genomics; PN-120233), to generate single cell gel beads in emulsion (GEMs). Sequencing libraries were generated with unique sample indices (SI) for each sample. The resulting libraries were assessed by gel electrophoresis (Agilent D1000 ScreenTape Assay) and quantified with qPCR (Illumina KAPA Library Quantification Kit). Following normalization to 2nM, libraries were denatured and diluted to 17pM of cluster generation using the Illumina cBot (HiSeq PE Cluster Kit v4).

text: normal

characteristic: disease

text: BRN3B-mCherry reporter

text: Homo sapiens

text: Homo sapiens

text: THY-negative

characteristic: phenotype

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: THY1 Negative Cells1


E-MTAB-6108:THY1 Positive Cells

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: School of Medicine, Menzies Institute for Medical Research, University of Tasmania, Hobart, Australia

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6108:THY1 Positive Cells

characteristic: Submitter Id

text: ArrayExpress

text: retinal ganglion cell

text: human

text: normal

text: BRN3B-mCherry reporter

text: Homo sapiens

text: Homo sapiens

text: THY-positive

text: embryonic stem cell

text: Homo sapiens

text: THY1 Positive Cells


E-MTAB-6727:B091 CD57+

characteristic: External Id

text: King's College London

text: public

characteristic: SRA accession

text: E-MTAB-6727:B091 CD57+

tag: Namespace:King's College London

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD8-Positive T-Lymphocytes

text: human

text: Protocols: Blood samples were taken from five HLA-A*24+ males with newly diagnosed type 1 diabetes. PBMCs were isolated on density gradients using Lymphoprep (Nycomed) and washed in RPMI supplemented with 2% human AB serum. PBMCs were washed and stained as above and CD57+ and CD57- effector memory CD8 T cells (gated as live CD14-CD16-CD19-CD4-CD3+CD8+CCR7-CD45RA-CD27-) were FACS-sorted using an Aria (BD Biosciences) into BSA supplemented with 10% BSA/5 mM EDTA. RNA was isolated immediately from cell pellets using the PicoPure RNA extraction kit (ThermoFisher). cDNA was generated and amplified using the SMARTseq v4 Ultra Low Input RNA Kit (Clontech) on the contactless Labcyte liquid handling system (Labcyte Inc). 200 ng of amplified cDNA was used from each sample where possible to generate libraries using the Ovation Ultralow Library System V2 kit (NuGEN). In brief, cDNA was fragmented through sonication on Covaris E220 (Covaris Inc), repaired and polished followed by ligation of indexed adapters. Adapter ligated cDNA was pooled before final amplification to add flow cell primers.

characteristic: description

text: type I diabetes mellitus

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: B091 CD57+

characteristic: title


E-MTAB-6727:B091 CD57-

characteristic: ENA-CHECKLIST

text: King's College London

characteristic: INSDC center name

text: public

text: E-MTAB-6727:B091 CD57-

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

characteristic: cell type

text: human

characteristic: common name

text: type I diabetes mellitus

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: phenotype

text: Homo sapiens

text: male

text: B091 CD57-


E-MTAB-6727:B115 CD57+

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: King's College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-6727:B115 CD57+

characteristic: Submitter Id

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: human

text: type I diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: B115 CD57+


E-MTAB-6727:B115 CD57-

characteristic: External Id

text: King's College London

text: public

characteristic: SRA accession

text: E-MTAB-6727:B115 CD57-

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD8-Positive T-Lymphocytes

text: human

characteristic: description

text: type I diabetes mellitus

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: B115 CD57-


E-MTAB-6727:G540 CD57+

characteristic: ENA-CHECKLIST

text: King's College London

characteristic: INSDC center name

text: public

text: E-MTAB-6727:G540 CD57+

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

characteristic: cell type

text: human

text: type I diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: G540 CD57+

characteristic: title


E-MTAB-6727:G540 CD57-

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: King's College London

text: public

text: E-MTAB-6727:G540 CD57-

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: human

characteristic: common name

text: type I diabetes mellitus

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: phenotype

text: Homo sapiens

text: male

text: G540 CD57-


E-MTAB-6727:G551 CD57+

characteristic: External Id

text: King's College London

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6727:G551 CD57+

characteristic: Submitter Id

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: human

text: type I diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: G551 CD57+


E-MTAB-6727:G551 CD57-

characteristic: ENA-CHECKLIST

text: King's College London

characteristic: INSDC center name

text: public

text: E-MTAB-6727:G551 CD57-

characteristic: age

text: ArrayExpress

characteristic: broker name

text: CD8-Positive T-Lymphocytes

text: human

characteristic: description

text: type I diabetes mellitus

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: G551 CD57-


E-MTAB-6727:G622 CD57+

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: King's College London

text: public

text: E-MTAB-6727:G622 CD57+

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

characteristic: cell type

text: human

text: type I diabetes mellitus

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: G622 CD57+

characteristic: title


E-MTAB-6727:G622 CD57-

characteristic: External Id

text: King's College London

characteristic: INSDC first public

text: public

text: E-MTAB-6727:G622 CD57-

text: ArrayExpress

text: CD8-Positive T-Lymphocytes

text: human

characteristic: common name

text: type I diabetes mellitus

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: phenotype

text: Homo sapiens

text: male

text: G622 CD57-


characteristic: ENA-CHECKLIST

text: INSERM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: antibody reference

text: human

text: siNT

characteristic: condition

characteristic: culture media

text: ChIP-seq

characteristic: experiment type

text: Homo sapiens

characteristic: protein target

characteristic: replicate number

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSERM

characteristic: INSDC first public

text: public

text: human

text: ChIP-seq

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: INSERM

text: public

text: human

characteristic: common name

text: ChIP-seq

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: jc2616_MCF7_FM_siTRPS1_H3K27ac_rep1_CRI01


text: INSERM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: siNT

text: input-seq

text: Homo sapiens

text: Homo sapiens


text: INSERM

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: antibody reference

text: human

text: ChIP-seq

text: Homo sapiens

text: Homo sapiens

text: jc2622_MCF7_FM_siTRPS1_H3K27ac_rep2_CRI01


text: INSERM

text: public

text: human

characteristic: condition

characteristic: culture media

text: ChIP-seq

characteristic: experiment type

text: Homo sapiens

text: Homo sapiens

text: jc2628_MCF7_FM_siTRPS1_H3K27ac_rep3_CRI01


characteristic: ENA-FIRST-PUBLIC

text: INSERM

text: public

text: human

text: ChIP-seq

text: Homo sapiens

text: ER

characteristic: protein target

characteristic: replicate number

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSERM

characteristic: INSDC first public

text: public

text: human

text: ChIP-seq

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: INSERM

text: public

text: human

characteristic: common name

text: ChIP-seq

text: Homo sapiens

text: CoREST

text: Homo sapiens

characteristic: title


text: INSERM

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: human

text: ChIP-seq

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: INSERM

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: input-seq

text: Homo sapiens

text: Homo sapiens


text: INSERM

text: public

characteristic: antibody reference

text: human

text: siNT

characteristic: condition

characteristic: culture media

text: ChIP-seq

text: Homo sapiens

text: ER

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: INSERM

text: public

text: human

text: ChIP-seq

characteristic: experiment type

text: Homo sapiens

text: ER

characteristic: protein target

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Animal and Plant Health Agency

text: public

tag: Namespace:Animal and Plant Health Agency

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Pheasant

characteristic: host scientific name

characteristic: isolate

text: Liver and Spleen

characteristic: isolation source

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Livestock associated methicillin resistant Staphylococcus aureus (LA-MRSA)


text: Staphylococcus aureus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Staphylococcus aureus

characteristic: common name

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: subject id

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Smurfit Institute of Genetics

text: public

characteristic: SRA accession

tag: Namespace:Smurfit Institute of Genetics

characteristic: Submitter Id

text: human

text: The Iceman diet stomach capture

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: The Iceman diet stomach capture


text: VANCOUVER PROSTATE CENTRE

text: public

tag: Namespace:VANCOUVER PROSTATE CENTRE

text: human

text: Homo sapiens

text: Homo sapiens

text: Tumor Sample


text: MPIEVB

text: public

text: F-CTATGCGCCTTGCCAGCCCGCTCAGTC;R-CGTATCGCCTCCCTCGCGCCATCAG

characteristic: adapters

characteristic: collection date

text: human

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Feces

characteristic: host body product

text: Feces

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: ACGAGTGCGTCA

characteristic: multiplex identifiers

text: 95°C, 5 min;30x[95°C, 55°C,72°C]45 sec;72°C, 4 min

characteristic: nucleic acid amplification

text: PMID: 18700153

characteristic: nucleic acid extraction

text: Homo sapiens

characteristic: pcr conditions

text: R-YRGAACAGGCTCCTCTAG;F-CAAACTGGGATTAGATACCC

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

text: The Iceman´s last meal consisted of fat, wild meat and cereals

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

unit: °C

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: 12S rRNA

characteristic: target gene

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: collection date

text: human

characteristic: common name

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Portion of intestinal secretion

characteristic: host body product

text: Transverse colon

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

text: PCR

text: ACGCTCGACACA

text: PMID: 18700153

text: Homo sapiens

characteristic: organism

text: death,mummification

text: collection 4°C

text: freezing -20°C

text: Archaeological Museum of Bolzano, Italy

text: Homo sapiens

text: pyrosequencing

text: 12S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: montane desert biome

text: ice mass

text: organic material

text: Italy

text: Portion of intestinal secretion

text: Duodenum

text: male

text: A

text: human-associated

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: AGACGCACTCCA

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: PMID: 18700153

characteristic: nucleic acid extraction

text: Homo sapiens

characteristic: pcr conditions

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: 12S rRNA

characteristic: target gene

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: collection date

text: human

characteristic: common name

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Portion of intestinal secretion

characteristic: host body product

text: Lumen of body of stomach

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

text: PCR

text: ATCAGACACGCA

text: PMID: 18700153

text: Homo sapiens

characteristic: organism

text: death,mummification

text: collection 4°C

text: freezing -20°C

text: Archaeological Museum of Bolzano, Italy

text: Homo sapiens

text: pyrosequencing

text: 12S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: montane desert biome

text: ice mass

text: organic material

text: Italy

text: Portion of intestinal secretion

text: Duodenum

text: male

text: A

text: human-associated

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: AGACGCACTCCA

characteristic: multiplex identifiers

characteristic: nucleic acid amplification

text: Homo sapiens

characteristic: pcr conditions

text: R-CTGGCCTCCAATTCATGTGAG;F-GCGTACGCAATCTTACGATCAA

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: cytB

characteristic: target gene

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: adapters

characteristic: collection date

text: human

characteristic: common name

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Portion of intestinal secretion

characteristic: host body product

text: Lumen of body of stomach

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

text: PCR

text: ATCAGACACGCA

text: Homo sapiens

characteristic: organism

text: death,mummification

text: collection 4°C

text: freezing -20°C

text: Archaeological Museum of Bolzano, Italy

text: Homo sapiens

text: pyrosequencing

text: cytB

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: montane desert biome

text: ice mass

text: organic material

text: Italy

text: Feces

text: Feces

text: male

text: A

text: human-associated

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: ACGAGTGCGTCA

characteristic: multiplex identifiers

text: PMID: 9665881

characteristic: nucleic acid amplification

text: Homo sapiens

text: PMID: 9665881

characteristic: pcr conditions

text: R-CGTCCTTTGTAACGATCAAG;F-GGCAGCATTCCGAGTAACTCCTC

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: rbcL

characteristic: target gene

text: large subunit

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: adapters

characteristic: collection date

text: human

characteristic: common name

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Portion of intestinal secretion

characteristic: host body product

text: Transverse colon

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

text: PCR

text: ACGCTCGACACA

text: PMID: 9665881

text: Homo sapiens

characteristic: organism

text: PMID: 9665881

text: death,mummification

text: collection 4°C

text: freezing -20°C

text: Archaeological Museum of Bolzano, Italy

text: Homo sapiens

text: pyrosequencing

text: rbcL

text: large subunit

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: montane desert biome

text: ice mass

text: organic material

text: Italy

text: Portion of intestinal secretion

text: Duodenum

text: male

text: A

text: human-associated

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: AGACGCACTCCA

characteristic: multiplex identifiers

text: PMID: 9665881

characteristic: nucleic acid amplification

text: Homo sapiens

text: PMID: 9665881

characteristic: pcr conditions

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: rbcL

characteristic: target gene

text: large subunit

characteristic: target subfragment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: adapters

characteristic: collection date

text: human

characteristic: common name

text: montane desert biome

characteristic: environment (biome)

text: ice mass

characteristic: environment (feature)

text: organic material

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Portion of intestinal secretion

characteristic: host body product

text: Lumen of body of stomach

characteristic: host body site

text: male

characteristic: host sex

text: A

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

text: PCR

text: ATCAGACACGCA

text: PMID: 9665881

text: Homo sapiens

characteristic: organism

text: PMID: 9665881

text: death,mummification

text: collection 4°C

text: freezing -20°C

text: Archaeological Museum of Bolzano, Italy

text: Homo sapiens

text: pyrosequencing

text: rbcL

text: large subunit

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIEVB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: montane desert biome

text: ice mass

text: organic material

text: Italy

text: Feces

text: Feces

text: male

text: A

text: human-associated

text: metagenome

characteristic: investigation type

text: PCR

characteristic: library construction method

text: ACGAGTGCGTCA

characteristic: multiplex identifiers

text: PMID: 17169982

characteristic: nucleic acid amplification

text: Homo sapiens

text: PMID: 17169982

characteristic: pcr conditions

text: R-CCATTGAGTCTCTGCACCTATC ;F-GGGCAATCCTGAGCCAA

characteristic: pcr primers

text: death,mummification

characteristic: perturbation

characteristic: project name

text: collection 4°C

characteristic: sample collection device or method

text: freezing -20°C

characteristic: sample material processing

text: Archaeological Museum of Bolzano, Italy

characteristic: sample storage location

characteristic: sample storage temperature

text: Homo sapiens

text: pyrosequencing

characteristic: sequencing method

text: trnL

characteristic: target gene

text: P6 loop

characteristic: target subfragment