characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E23

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E24

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E2

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E3

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E4

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E5

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E6

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E7

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E8

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_E9

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F10

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F11

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F12

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F13

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F14

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F15

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F16

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F17

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F18

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F19

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F1

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F20

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F21

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F22

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F23

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F24

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F2

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F3

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F4

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F5

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F7

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F8

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_F9

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G10

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G11

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G12

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G13

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G14

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G15

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G16

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G17

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G18

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G19

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G1

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G20

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G21

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G22

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G23

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H13

characteristic: Submitter Id

text: ArrayExpress

text: adult insulin secreting cell of pars intercerebralis

characteristic: cell type

text: fruit fly

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H14

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_G9

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: cell type

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H16

text: ArrayExpress

text: fruit fly

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H17

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: dilp2-GAL4; UAS-unc84GFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: head

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H18

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: cell type

text: fruit fly

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H19

text: ArrayExpress

text: fruit fly

text: adult

text: dilp2-GAL4; UAS-unc84GFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: head

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P55_female_dilp2IPC_H1

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: dilp2-GAL4; UAS-unc84GFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: head

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H11

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H13

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H15

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H16

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H18

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H19

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H20

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H21

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H23

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H24

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H3

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H4

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H7

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H8

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_H9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I10

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I11

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I12

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I13

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I14

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I16

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I17

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I18

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I19

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I1

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I20

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I21

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I22

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I24

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I2

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I4

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I5

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I7

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I8

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_I9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J10

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J12

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J14

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J13

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J16

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J17

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J19

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P32_male_fatbody_J18

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


E-MTAB-10650:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: E-MTAB-10650:Sample 1

tag: Namespace:Institute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Giessen, Germany

text: ArrayExpress

characteristic: broker name

text: stiff brome

characteristic: common name

text: Bd 21-3

characteristic: cultivar

text: Protocols: Brachypodium distachyon roots were pooled in biological samples (three roots per sample). Si mycelium and spores were collected from four-week-old axenic cultures grown on CM medium. All samples in triplicates were shock frozen, stored at -80°C and ground in liquid N2. The seeds of Brachypodium distachyon (Bd) line Bd21-3 (gift from R. Sibout, INRA Versailles, France) were surface sterilized (3% active chlorine, sodium hypochlorite solution) for 15 min, washed three times and placed on half-strength MS medium in dark at 4°C for two days and then seven days at 24°C and 16h light / 8h dark cycle (47 μmol m−2 s−1 photon flux density). Serendipita indica (Si) (IPAZ-11827, Institute of Phytopathology, Giessen, Germany) was grown on complete media plates (CM) at 23°C in dark for 4-5 weeks. For inoculation, Si mycelium was collected in 0.002% aqueous Tween 20 solution, filtered (Miracloth, Calbiochem) and pelleted by centrifugation (10 min/4000 rpm/20°C) twice. Chlamydospore concentration of 500,000 conidia ml-1 in 0.002% Tween 20 solution was used to inoculate seven-day-old plant seedlings for 2-3 h. Control plants were mock treated with the 0.002% Tween 20 solution for the same time. Brachypodium distachyon roots inoculated with Serendipita indica or mock-inoculated in Tween water (0.002% Tween 20 solution) were grown on a mixture (2:1, v/v) of vermiculite (Deutsche Vermiculite GmbH) and oil dri (oil binder Typ III R Coarse grain, Damolin, Mettmann, Germany) under 16 h light (160 μmol m-2 s-1, 22°C) and 8h dark (18°C) conditions at 60% relative humidity for four days. Total RNA was isolated using the ZymoBIOMICS RNA Mini Kit (Zymo Research, USA), quantified with DropSense16/Xpose (BIOKÉ, Netherlands) and analyzed with an Agilent 2100 Bioanalyzer Nano Chip (Agilent, Germany). RNA Clean and Concentrator 25 and 5 kits (Zymo Research) were utilized to separate total RNA into fractions: 17-200 nt and > 200 nt. Quantity and quality of the smaller RNA fractions were assessed with the Qubit fluorometer (Invitrogen, Germany) and Agilent 2100 Bioanalyzer Pico Chip. sRNA library preparation was done with 50 ng of RNA (TruSeq Small RNA Library Prep, Illumina) and size selection with the BluePippin (Sage Science, USA) for fragments between 140 and 160 nt (15-35 nt without adapters) applied.

characteristic: description

text: mock

characteristic: infect

text: Brachypodium distachyon

characteristic: organism

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 1


E-MTAB-10650:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Giessen, Germany

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10650:Sample 2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: mock

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 2


E-MTAB-10650:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10650:Sample 3

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: mock

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

characteristic: organism part

text: Brachypodium distachyon

text: Sample 3

characteristic: title


E-MTAB-10650:Sample 4

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: E-MTAB-10650:Sample 4

text: ArrayExpress

characteristic: broker name

text: stiff brome

text: Bd 21-3

text: Serendipita indica

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 4


E-MTAB-10650:Sample 5

text: public

text: E-MTAB-10650:Sample 5

text: ArrayExpress

text: stiff brome

characteristic: common name

text: Bd 21-3

characteristic: cultivar

characteristic: description

text: Serendipita indica

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 5


E-MTAB-10650:Sample 6

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10650:Sample 6

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: Serendipita indica

characteristic: infect

text: Brachypodium distachyon

characteristic: organism

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 6


E-MTAB-10650:Sample 7

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10650:Sample 7

text: ArrayExpress

text: Serendipita indica

text: Serendipita indica

text: mycelium

text: Serendipita indica

text: Sample 7


E-MTAB-10650:Sample 8

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10650:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: Serendipita indica

text: Serendipita indica

text: mycelium

text: Serendipita indica

text: Sample 8


E-MTAB-10650:Sample 9

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10650:Sample 9

characteristic: age

text: ArrayExpress

text: Serendipita indica

text: Serendipita indica

text: mycelium

characteristic: organism part

text: Serendipita indica

text: Sample 9


E-MTAB-10649:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: E-MTAB-10649:Sample 1

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: Protocols: Brachypodium distachyon roots were pooled in biological samples (three roots per sample). Si mycelium and spores were collected from four-week-old axenic cultures grown on CM medium. All samples in triplicates were shock frozen, stored at -80°C and ground in liquid N2. The seeds of Brachypodium distachyon (Bd) line Bd21-3 (gift from R. Sibout, INRA Versailles, France) were surface sterilized (3% active chlorine, sodium hypochlorite solution) for 15 min, washed three times and placed on half-strength MS medium in dark at 4°C for two days and then seven days at 24°C and 16h light / 8h dark cycle (47 μmol m−2 s−1 photon flux density). Serendipita indica (Si) (IPAZ-11827, Institute of Phytopathology, Giessen, Germany) was grown on complete media plates (CM) at 23°C in dark for 4-5 weeks. For inoculation, Si mycelium was collected in 0.002% aqueous Tween 20 solution, filtered (Miracloth, Calbiochem) and pelleted by centrifugation (10 min/4000 rpm/20°C) twice. Chlamydospore concentration of 500,000 conidia ml-1 in 0.002% Tween 20 solution was used to inoculate seven-day-old plant seedlings for 2-3 h. Control plants were mock treated with the 0.002% Tween 20 solution for the same time.Brachypodium distachyon roots inoculated with Serendipita indica or mock-inoculated in Tween water (0.002% Tween 20 solution) were grown on a mixture (2:1, v/v) of vermiculite (Deutsche Vermiculite GmbH) and oil dri (oil binder Typ III R Coarse grain, Damolin, Mettmann, Germany) under 16 h light (160 μmol m-2 s-1, 22°C) and 8h dark (18°C) conditions at 60% relative humidity for four days. Total RNA was isolated using the ZymoBIOMICS RNA Mini Kit (Zymo Research, USA), quantified with DropSense16/Xpose (BIOKÉ, Netherlands) and analyzed with an Agilent 2100 Bioanalyzer Nano Chip (Agilent, Germany). RNA Clean and Concentrator 25 and 5 kits (Zymo Research) were utilized to separate total RNA into fractions: 17-200 nt and > 200 nt. 1.5 μg of the larger fractions were processed for mRNA library preparation (TruSeq Stranded mRNA protocol, Illumina, USA). Fragment Analyzer Automated CE System (Advanced Analytical Technologies, Austria) determined the quality of the generated polyA mRNA libraries.

text: mock

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 1

characteristic: title


E-MTAB-10649:Sample 2

text: public

text: E-MTAB-10649:Sample 2

text: ArrayExpress

characteristic: broker name

text: stiff brome

characteristic: common name

text: Bd 21-3

characteristic: cultivar

text: mock

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 2


E-MTAB-10649:Sample 3

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10649:Sample 3

text: ArrayExpress

text: stiff brome

text: Bd 21-3

characteristic: description

text: mock

characteristic: infect

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 3


E-MTAB-10649:Sample 4

characteristic: External Id

characteristic: INSDC first public

text: public

text: E-MTAB-10649:Sample 4

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: Serendipita indica

text: Brachypodium distachyon

characteristic: organism

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 4


E-MTAB-10649:Sample 5

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10649:Sample 5

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: Serendipita indica

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 5


E-MTAB-10649:Sample 6

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10649:Sample 6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: stiff brome

text: Bd 21-3

text: Serendipita indica

text: Brachypodium distachyon

text: Brachypodium distachyon

text: root

text: Brachypodium distachyon

text: Sample 6


E-MTAB-10649:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: E-MTAB-10649:Sample 7

text: ArrayExpress

text: Serendipita indica

text: Serendipita indica

text: mycelium

characteristic: organism part

text: Serendipita indica

text: Sample 7


E-MTAB-10649:Sample 8

text: public

text: E-MTAB-10649:Sample 8

text: ArrayExpress

characteristic: broker name

text: Serendipita indica

text: Serendipita indica

text: mycelium

text: Serendipita indica

text: Sample 8

characteristic: title


E-MTAB-10649:Sample 9

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10649:Sample 9

text: ArrayExpress

text: Serendipita indica

text: Serendipita indica

text: mycelium

text: Serendipita indica

text: Sample 9


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J1

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J20

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J21

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J22

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J23

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J24

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J2

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J3

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J5

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J6

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J7

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J8

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_J9

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K11

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K12

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K13

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K14

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K15

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K16

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K17

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K18

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K19

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K1

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K20

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K21

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K22

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K23

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K24

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K2

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K3

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K4

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K5

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: External Id

text: BCM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K6

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: fat body

text: pass

text: Drosophila melanogaster

text: male

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

characteristic: INSDC center name

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K7

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: Cg-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: male

text: OK

characteristic: title


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P32_male_fatbody_K8

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

characteristic: developmental stage

text: Cg-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: fat body

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain