text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H21

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H24

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_H9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I13

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I15

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I16

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I19

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I21

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I24

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I8

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_I9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J13

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J16

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J19

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J21

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J24

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_J9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K10

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K13

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K16

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K18

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K19

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K21

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K24

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624976556:FCA_P9_female_bodyWall_K7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: public

tag: Namespace:Department of Molecular Microbiology John Innes Centre

text: ArrayExpress

text: Protocols: Streptomyces venezuelae strain NRRL B-65442 (NCBI Ref: NZ_CP018074.1) was used as the wild type strain. The WhiB deletion strain was made using 'Redirect' PCR targeting (Gust et al., 2003; Gust et al., 2004), to replace the WhiB gene with a single apramycin resistance (apr) cassette in the wild type Streptomyces venezuelae strain. The ΔwhiB::apr locus was transduced back into wild type S. venezuelae using the transducing phage SV1 (Stuttard et al., 1982)to generate the whiB deletion strain. Formaldehyde was added to cultures at a final concentration of 1% (vol/vol), and incubation was continued for 30 min. Glycine was then added to a final concentration of 125 mM to stop the cross-linking. The samples were left at room temperature for 5 min and washed twice in phosphate-buffered saline (PBS) buffer (pH 7.4). The pellets were resuspended in 0.5 ml of lysis buffer (10 mM Tris-Cl, pH 8.0, 50 mM NaCl), containing 15 mg/ml of lysozyme and 1x protease inhibitor (Roche Applied Science) and incubated at 37 degree C for 25 min or until lysed. Subsequently, 0.5 ml immunoprecipitation (IP) buffer (50 mM Tris-Cl, pH 8.0, 250 mM NaCl, 0.8% [vol/vol] Triton X-100), containing 1x protease inhibitor, was added, and the samples were chilled on ice. The samples were sonicated for six cycles of 20 s each at 10 micrometer to shear chromosomal DNA into fragments ranging from 300 to 1,000 bp on average. The samples were centrifuged twice at 16,000g at 4 degree C for 10 min to clear the cell extracts. For total DNA extraction, 50 microlitres of the cell extracts were incubated with 0.2 mg/ml proteinase K (Roche) for 2 h at 55 degree C, then extracted twice with phenol-chloroform and once with chloroform and further purified using QiaQuick columns (Qiagen). DNA was eluted in 50 microlitres EB buffer (10 mM Tris-Cl, pH 8.5) and quantified using a NanoDrop spectrophotometer (Thermo Scientific). Library construction and sequencing were performed by The Genome Analysis Centre (TGAC), Norwich Research Park, Norwich, United Kingdom. The TruSeq ChIP sample preparation kit from Illumina Inc. (part 15023092, revision A, August 2012) was used according to the recommended protocol. In brief, 5 to 10 ng ChIP DNA is blunt ended and phosphorylated, and a single A nucleotide is added to the 3 ends of the fragments in preparation for ligation to an adapter with a single-base T overhang. The ligation products are purified and accurately size selected by agarose gel electrophoresis. Size-selected DNA is then purified and PCR amplified to enrich for fragments that have adapters on both ends. The final purified product is then quantified using a combination of Bioanalyzer DNA HS Chip from Agilent Technology 2100 Bioanalyzer and Qubit 2.0 from Invitrogen. The libraries were normalized to 10 nM and pooled, and quantitative PCR (q-PCR) was performed prior to cluster generation.

text: deletion of whib

characteristic: genetic modification

text: Streptomyces venezuelae

characteristic: organism

text: Streptomyces venezuelae

text: Streptomyces venezuelae

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Molecular Microbiology John Innes Centre

text: public

text: ArrayExpress

text: deletion of whib

text: Streptomyces venezuelae

text: Streptomyces venezuelae

text: Streptomyces venezuelae


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: flag tag whib

text: Streptomyces venezuelae

text: Streptomyces venezuelae

text: Streptomyces venezuelae


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: Streptomyces venezuelae

text: Streptomyces venezuelae

text: Streptomyces venezuelae


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Alderley Park

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Lighthouse Lab in Alderley Park by Jacquelyn Wynn, Mairead Hyland, The Lighthouse Lab in Alderley Park and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-CHECKLIST

text: SC

text: public

text: SC

text: Lighthouse Laboratory Plymouth

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: Wales

text: Human

characteristic: organism

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: Samples prepared at Lighthouse Lab in Milton Keynes by The Lighthouse Lab in Milton Keynes and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human


text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Lab in Glasgow

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Alderley Park

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

text: England

text: Human


text: SC

text: public

text: SC

text: Health Services Laboratories

text: Samples prepared at Health Services Laboratories by Health Services Laboratories and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

characteristic: sample capture status


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_K8

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_K9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L10

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L13

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L16

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L19

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L21

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L23

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L4

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L7

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_L9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M10

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M13

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M16

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M19

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M21

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M23

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M4

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M7

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_M9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N10

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N11

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N13

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N16

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N19

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N21

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N23

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N4

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: body wall

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281624978684:FCA_P9_female_bodyWall_N7

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: body wall

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK