E-MTAB-10183:Sample 23

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 23

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: Isolation of the mouse stromal vascular fraction (SVF) Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into PBS. The tissues were subsequently minced using scissors, transferred into collagenase (Sigma-Aldrich #C6885-1G, 2 mg/ml of homemade collagenase buffer (25 mM NaHCO3, 12 mM KH2PO4, 1.2 m MgSO4, 4.8 mM KCl, 120 mM NaCl, 1.4 M CaCl2, 5 mM Glucose, 2.5% BSA, pH = 7.4)) and incubated for 65-70 min at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45-50 min of digestion. The cell suspension was then diluted with PBS (with the aim of diluting the collagenase and stopping/slowing down the digestion), and filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell suspension. Next, the cells were pelleted by a 5 min centrifugation at 400 g at room temperature and red blood cells were lysed by incubating the pellet with a homemade red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for a maximum of 5 min, followed by two washes (a 5 min centrifugation at 400 g, room temperature) with a homemade FACS buffer (PBS with 3% foetal bovine serum (FBS) (Gibco #10270-106), 1 mM EDTA, 1% penicillin-streptomycin (Gibco #15140122)). FACS-based cell isolation The SVF cell suspension was diluted to 1 × 10^7 cells/ml with a homemade FACS buffer (see above) and the following fluorophore-conjugated antibodies were added (in titration-determined quantities, Suppl. Table 3): anti-mouse CD31-AF488, anti-mouse CD45-AF488, anti-mouse TER119-AF488 (BioLegend #303110, #304017 and #116215, respectively) to select the Lin− population; anti-mouse SCA1-PE-Cy7 (BioLegend #122513) to enrich the Lin− population with ASPCs, and anti-mouse CD142-PE (#FAB3178P (RnD). The cells were incubated with the antibodies on ice for at least 30 min and protected from light, after which they were washed with the FACS buffer, stained with propidium iodide (Molecular Probes #P3566) for assessing viability, filtered through a 40-µm cell strainer and subjected to FACS with the use of a Becton Dickinson FACSAria II sorter. Compensation measurements were performed for single stains using positive/negative compensation beads (eBiosciences #01-2222-42). The following gating strategy was applied while sorting the cells: first, the cells were selected based on their size and granularity/complexity (side and forward scatter), followed by a double elimination of any events that could represent more than one cell. Next, the Lin−(CD31− CD45− TER119-) population was selected, followed by a Lin−SCA1+ selection (ASPCs), and finally, ASPC cells that were negative or positive for CD142 marker: Lin− SCA1+ CD142− (CD142− ASPCs) and Lin− SCA1+ CD142+ (CD142+ ASPCs, Aregs). Wild-type C57BL/6J male and female adult mice were euthanised at CO2. Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into ice cold PBS for further processing. siRNA-mediated knockdown experiments For each gene, a pool of 3 siRNA probes (IDT, TriFECTA DsiRNAs, Suppl. Table 4) was reverse-transfected to CD142+ ASPCs (Aregs) or total ASPCs. Seventy-five thousand cells/cm2 were plated into the transwell well with previously deposited siRNA mix containing 20 nM of each siRNA re-suspended in 1.5% Lipofectamine RNAiMAX (Invitrogen #13778150) dissolved in Opti-MEM I reduced serum medium (Invitrogen #31985062), and high glucose DMEM medium supplemented with 2.5% FBS (w/o penicillin-streptomycin). After 24 h, the medium was changed to high glucose DMEM medium supplemented with 10% FBS and 1% penicillin-streptomycin and after 48 h the cells were put together with the receiving CD142− ASPCs below the transwell and both populations were induced with white adipogenic differentiation cocktail (see ‘Treatment protocol). In vitro adipogenic differentiation The CD142+ ASPCs (Aregs) carrying the indicated knockdowns and the CD142− ASPCs below the transwell were treated with a single dose of white adipocyte differentiation induction cocktail (0.5 µM 3-isobutyl-1-methylxanthine (IBMX, Sigma #15879), 1 μM dexamethasone (Sigma #D2915), 170 nM (1µg/ml) insulin (Sigma #19278)), followed by a maintenance treatment two-three days post-induction (170 nM insulin) and refreshed every two-three days. The culture was carried out until day six-eight after induction, at which point cells were stained for imaging or collected for RNA extraction. CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying siRNA-mediated knockdown constructs were collected into Tri-Reagent (Molecular Research Center #TR118) after 6-8 days of adipogenic differentiation. Direct-zol RNA kit (Zymo Research #R2052) was used to extract RNA according to the manufacture’s specifications. 3’end bulk mRNA sequencing was performed following the BRB-seq strategy as previously described (Alpern et al., 2019). In brief, 7-200 ng of total RNA from each sample was reverse transcribed in a 96-well plate using SuperScriptTM II Reverse Transcriptase (Lifetech 18064014) with individual barcoded oligo-dT primers, featuring a 12-nt-long sample barcode (IDT). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method. For that, a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E. coli DNA ligase (NEB, #M0205 L), 5 μl of E. coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (10 mM), 10 μl of 5x Second Strand Buffer (100 mM Tris, pH 6.9, (AppliChem, #A3452), 25 mM MgCl2 (Sigma, #M2670), 450 mM KCl (AppliChem, #A2939), 0.8 mM β-NAD (Sigma, N1511), 60 mM (NH4)2SO4 (Fisher Scientific Acros, #AC20587), and 11 μl of water was added to 20 μl of ExoI-treated first-strand reaction on ice. The reaction was incubated at 16 °C for 2.5 h. Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water. The Illumina-compatible libraries were prepared by tagmentation of 10-40 ng of full-length double-stranded cDNA with 1 µl of in-house produced Tn5 enzyme (11 μM). After tagmentation, the libraries where purified with DNA Clean and Concentrator kit (Zymo Research #D4014), eluted in 20 µl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of each i5 and i7 Illumina index adapter (IDT) following this program: incubation 72 °C-3 min, denaturation 98 °C-30 s; 15 cycles: 98 °C-10 s, 63 °C-30 s, 72 °C-30 s; final elongation at 72 °C-5 min. The libraries were purified twice with AMPure beads (Beckman Coulter, #A63881) at 0.6x ratio to remove the fragments < 300 nt. The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 (Illumina).

text: adult

text: wild type genotype

text: CD142- ASPCs co-cultured and co-differentiated (in a transwell set up) without Aregs (CD142+ ASPCs)

characteristic: growth condition

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: not applicable (empty transwell)

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 23

characteristic: title


E-MTAB-10183:Sample 24

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 24

text: over 8

unit: week

text: ArrayExpress

characteristic: broker name

text: adipose tissue derived mesenchymal stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 2

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: not applicable (empty transwell)

text: Sample 24


E-MTAB-10183:Sample 25

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 25

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: not applicable (empty transwell)

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 25

characteristic: title


E-MTAB-10183:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 5

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: not applicable (empty transwell)

text: Sample 5


E-MTAB-10183:Sample 1

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

text: E-MTAB-10183:Sample 1

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: CD142- ASPCs co-cultured and co-differentiated (in a transwell set up) with Aregs (CD142+ ASPCs) carrying siRNA-mediated specific gene knockdown

text: pool 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: scrambled

text: Sample 1


E-MTAB-10183:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 2

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: scrambled

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 2

characteristic: title


E-MTAB-10183:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 3

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: scrambled

text: Sample 3


E-MTAB-10183:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10183:Sample 9

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: scrambled

text: Sample 9


E-MTAB-10183:Sample 19

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 19

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 19

characteristic: title


E-MTAB-10183:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 20

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 20


E-MTAB-10183:Sample 21

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10183:Sample 21

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 21


E-MTAB-10183:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 26

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 26

characteristic: title


E-MTAB-10183:Sample 14

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 14

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 14


E-MTAB-10183:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10183:Sample 6

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 6


E-MTAB-10183:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 7

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-10183:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 8

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 8


E-MTAB-10183:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10183:Sample 16

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 16


E-MTAB-10183:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 17

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 17

characteristic: title


E-MTAB-10183:Sample 18

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 18

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 18


E-MTAB-10183:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10183:Sample 22

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 22


E-MTAB-10183:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 11

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: siMgp

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 11

characteristic: title


E-MTAB-10183:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 12

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: siMgp

text: Sample 12


E-MTAB-10183:Sample 13

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10183:Sample 13

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: siMgp

text: Sample 13


E-MTAB-10183:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10183:Sample 15

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: growth condition

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: siMgp

characteristic: siRNA of CD142+ APSCs

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-10183:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10183:Sample 10

text: over 8

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 10


E-MTAB-10183:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10183:Sample 4

text: over 8

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 4


E-MTAB-10184:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 1

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: Protocols: Isolation of the mouse stromal vascular fraction (SVF) Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into PBS. The tissues were subsequently minced using scissors, transferred into collagenase (Sigma-Aldrich #C6885-1G, 2 mg/ml of homemade collagenase buffer (25 mM NaHCO3, 12 mM KH2PO4, 1.2 m MgSO4, 4.8 mM KCl, 120 mM NaCl, 1.4 M CaCl2, 5 mM Glucose, 2.5% BSA, pH = 7.4)) and incubated for 65-70 min at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45-50 min of digestion. The cell suspension was then diluted with PBS (with the aim of diluting the collagenase and stopping/slowing down the digestion), and filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell suspension. Next, the cells were pelleted by a 5 min centrifugation at 400 g at room temperature and red blood cells were lysed by incubating the pellet with a homemade red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for a maximum of 5 min, followed by two washes (a 5 min centrifugation at 400 g, room temperature) with a homemade FACS buffer (PBS with 3% foetal bovine serum (FBS) (Gibco #10270-106), 1 mM EDTA, 1% penicillin-streptomycin (Gibco #15140122)). FACS-based cell isolation The SVF cell suspension was diluted to 1 × 10^7 cells/ml with a homemade FACS buffer (see above) and the following fluorophore-conjugated antibodies were added (in titration-determined quantities, Suppl. Table 3): anti-mouse CD31-AF488, anti-mouse CD45-AF488, anti-mouse TER119-AF488 (BioLegend #303110, #304017 and #116215, respectively) to select the Lin− population; anti-mouse SCA1-PE-Cy7 (BioLegend #122513) to enrich the Lin− population with ASPCs, and anti-mouse CD142-PE (# ORB507485) The cells were incubated with the antibodies on ice for at least 30 min and protected from light, after which they were washed with the FACS buffer, stained with propidium iodide (Molecular Probes #P3566) for assessing viability, filtered through a 40-µm cell strainer and subjected to FACS with the use of a Becton Dickinson FACSAria II sorter. Compensation measurements were performed for single stains using positive/negative compensation beads (eBiosciences #01-2222-42). The following gating strategy was applied while sorting the cells: first, the cells were selected based on their size and granularity/complexity (side and forward scatter), followed by a double elimination of any events that could represent more than one cell. Next, the Lin−(CD31− CD45− TER119-) population was selected, followed by a Lin−SCA1+ selection (ASPCs), and finally, ASPC cells that were negative or positive for CD142 marker: Lin− SCA1+ CD142− (CD142− ASPCs) and Lin− SCA1+ CD142+ (CD142+ ASPCs, Aregs). Wild-type C57BL/6J male and female adult mice were euthanised at CO2. Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into ice cold PBS for further processing. Equal numbers pf CD142− ASPCs were sorted and plated in flat-bottom microscopy-adapted cell culture plates (Corning #353219), cultured in high glucose DMEM medium (Gibco #61965026) supplemented with 10% FBS and 1% penicillin-streptomycin. At confluence, the cells were induced with white adipogenic differentiation cocktail supplemented with the indicated compounds (see ‘Treatment protocol). In vitro adipogenic differentiation, retinoic acid (RA) and EGF treatment CD142− ASPCs at confluence were treated with a single dose of white adipocyte differentiation induction cocktail (0.5 µM 3-isobutyl-1-methylxanthine (IBMX, Sigma #15879), 1 μM dexamethasone (Sigma #D2915), 170 nM (1µg/ml) insulin (Sigma #19278)) supplemented with DMSO (RA carrier), different concentrations of RA (0.01, 0.1, 1, 10, 100 µM) and EGF (100 ng/ml). Induction was followed by a maintenance treatment two-three days post-induction (170 nM insulin) supplemented with the same amounts of DMSO, RA and EGF as indicated above. The culture was carried out until day six-eight after induction, at which point cells were stained for imaging or collected for RNA extraction. CD142− ASPCs having been exposed to the adipogenic differentiation cocktail supplemented with the indicated compounds for 6-8 days, were collected into Tri-Reagent (Molecular Research Center #TR118). Direct-zol RNA kit (Zymo Research #R2052) was used to extract RNA according to the manufacture’s specifications. 3’end bulk mRNA sequencing was performed following the BRB-seq strategy as previously described (Alpern et al., 2019). In brief, 7-200 ng of total RNA from each sample was reverse transcribed in a 96-well plate using SuperScriptTM II Reverse Transcriptase (Lifetech 18064014) with individual barcoded oligo-dT primers, featuring a 12-nt-long sample barcode (IDT). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method. For that, a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E. coli DNA ligase (NEB, #M0205 L), 5 μl of E. coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (10 mM), 10 μl of 5x Second Strand Buffer (100 mM Tris, pH 6.9, (AppliChem, #A3452), 25 mM MgCl2 (Sigma, #M2670), 450 mM KCl (AppliChem, #A2939), 0.8 mM β-NAD (Sigma, N1511), 60 mM (NH4)2SO4 (Fisher Scientific Acros, #AC20587), and 11 μl of water was added to 20 μl of ExoI-treated first-strand reaction on ice. The reaction was incubated at 16 °C for 2.5 h. Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water. The Illumina-compatible libraries were prepared by tagmentation of 10-40 ng of full-length double-stranded cDNA with 1 µl of in-house produced Tn5 enzyme (11 μM). After tagmentation, the libraries where purified with DNA Clean and Concentrator kit (Zymo Research #D4014), eluted in 20 µl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of each i5 and i7 Illumina index adapter (IDT) following this program: incubation 72 °C-3 min, denaturation 98 °C-30 s; 15 cycles: 98 °C-10 s, 63 °C-30 s, 72 °C-30 s; final elongation at 72 °C-5 min. The libraries were purified twice with AMPure beads (Beckman Coulter, #A63881) at 0.6x ratio to remove the fragments < 300 nt. The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 (Illumina).

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-10184:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 5

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 5


E-MTAB-10184:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 9

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 3

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 9


E-MTAB-10184:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 10

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 10

characteristic: title


E-MTAB-10184:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 2

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 2


E-MTAB-10184:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 6

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 2

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 6


E-MTAB-10184:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 11

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 11

characteristic: title


E-MTAB-10184:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 3

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 3


E-MTAB-10184:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 7

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 2

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 7


E-MTAB-10184:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 14

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 14

characteristic: title


E-MTAB-10184:Sample 21

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 21

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 21


E-MTAB-10184:Sample 28

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 28

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 6

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 28


E-MTAB-10184:Sample 35

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 35

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 7

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 35

characteristic: title


E-MTAB-10184:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 12

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 12


E-MTAB-10184:Sample 4

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 4

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 4


E-MTAB-10184:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 8

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 8

characteristic: title


E-MTAB-10184:Sample 13

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 13

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 13


E-MTAB-10184:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 20

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 5

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 20


E-MTAB-10184:Sample 27

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 27

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 6

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 27

characteristic: title


E-MTAB-10184:Sample 34

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 34

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 7

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 34


E-MTAB-10184:Sample 15

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 15

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 4

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 15


E-MTAB-10184:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 22

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 22

characteristic: title


E-MTAB-10184:Sample 29

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 29

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 6

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 29


E-MTAB-10184:Sample 36

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 36

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 7

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 36


E-MTAB-10184:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 16

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 16

characteristic: title


E-MTAB-10184:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 23

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 23


E-MTAB-10184:Sample 30

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 30

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 6

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 30


E-MTAB-10184:Sample 37

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 37

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 7

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 37

characteristic: title


E-MTAB-10184:Sample 17

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 17

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 17


E-MTAB-10184:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 24

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 5

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 24


E-MTAB-10184:Sample 31

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 31

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 6

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 31

characteristic: title


E-MTAB-10184:Sample 38

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 38

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 7

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 38


E-MTAB-10184:Sample 18

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 18

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 4

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 18


E-MTAB-10184:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 25

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 25

characteristic: title


E-MTAB-10184:Sample 32

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 32

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 6

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 32


E-MTAB-10184:Sample 39

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

text: E-MTAB-10184:Sample 39

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 7

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 39


E-MTAB-10184:Sample 19

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 19

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 19

characteristic: title


E-MTAB-10184:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10184:Sample 26

text: over 8

text: ArrayExpress

characteristic: broker name

text: adipose stem and progenitor cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 26


E-MTAB-10184:Sample 33

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: EPFL

text: public

text: E-MTAB-10184:Sample 33

text: over 8

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

text: pool 6

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 33


E-MTAB-10184:Sample 40

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: EPFL

text: public

characteristic: SRA accession

text: E-MTAB-10184:Sample 40

characteristic: Submitter Id

text: over 8

characteristic: age

text: ArrayExpress

text: adipose stem and progenitor cell

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 7

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: phenotype

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 40

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: WETSUS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

characteristic: common name

text: soil

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: eukaryote

characteristic: investigation type

text: uncultured fungus

characteristic: project name

text: uncultured fungus

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WETSUS

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: S

text: S

characteristic: description

text: soil

text: soil

text: soil

text: Netherlands

text: host-associated

text: eukaryote

text: uncultured fungus

characteristic: organism

text: uncultured fungus

text: Illumina MiSeq

text: S

characteristic: title


text: WETSUS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: st.S

characteristic: common name

text: st.S

text: soil

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Netherlands

text: host-associated

text: eukaryote

text: uncultured fungus

text: uncultured fungus

text: Illumina MiSeq

text: st.S


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WETSUS

text: public

text: st.S+ st.C0

text: st.S+ st.C0

text: soil

text: soil

text: soil

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: eukaryote

characteristic: investigation type

text: uncultured fungus

characteristic: project name

text: uncultured fungus

text: Illumina MiSeq

characteristic: sequencing method

text: st.S+ st.C0


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

tag: Namespace:Tromsoe University Museum

characteristic: Submitter Id

text: Alm, Torbjorn; Often, Anders

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Stuckenia filiformis

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

characteristic: plant structure

text: Stuckenia filiformis

text: Tromsoe herbarium specimen TROM_V_100105

characteristic: source material identifiers

text: TROM_V_100105 herbarium sample of Stuckenia filiformis

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Lanes, Kristian

characteristic: collection date

text: Norway maple

characteristic: common name

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Acer platanoides

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Acer platanoides

text: Tromsoe herbarium specimen TROM_V_100387

text: TROM_V_100387 herbarium sample of Acer platanoides


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Lauritz Holm-Nielsen, Peder Pedersen,

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Papaver dubium

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Papaver dubium

text: Tromsoe herbarium specimen TROM_V_100672

text: TROM_V_100672 herbarium sample of Papaver dubium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Engelskjon, Torstein; Vagen, Inger

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Carex elongata

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Carex elongata

text: Tromsoe herbarium specimen TROM_V_100852

characteristic: source material identifiers

text: TROM_V_100852 herbarium sample of Carex elongata

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Engelskjon, Torstein

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Antennaria villifera

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Antennaria villifera

text: Tromsoe herbarium specimen TROM_V_102189

text: TROM_V_102189 herbarium sample of Antennaria villifera


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Molster, Liv; Johansen, Bernt; Rohde, Torfinn

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Arenaria pseudofrigida

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Arenaria pseudofrigida

text: Tromsoe herbarium specimen TROM_V_102360

text: TROM_V_102360 herbarium sample of Arenaria pseudofrigida


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Barfod, Knud

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

text: Carex pilulifera

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Carex pilulifera

text: Tromsoe herbarium specimen TROM_V_103356

characteristic: source material identifiers

text: TROM_V_103356 herbarium sample of Carex pilulifera

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Sortland, Andy

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Anthemis arvensis

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Anthemis arvensis

text: Tromsoe herbarium specimen TROM_V_1056

text: TROM_V_1056 herbarium sample of Anthemis arvensis


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Elven, Reidar

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Puccinellia phryganodes

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Puccinellia phryganodes

text: Tromsoe herbarium specimen TROM_V_106771

text: TROM_V_106771 herbarium sample of Puccinellia phryganodes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Knutz, Harald

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

text: Juncus acutiflorus

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Juncus acutiflorus

text: Tromsoe herbarium specimen TROM_V_106866

characteristic: source material identifiers

text: TROM_V_106866 herbarium sample of Juncus acutiflorus

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Benum, Peter

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Echinochloa crus-galli

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Echinochloa crus-galli

text: Tromsoe herbarium specimen TROM_V_108162

text: TROM_V_108162 herbarium sample of Echinochloa crus-galli


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Pedersen, Geir

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Digitaria sanguinalis

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Digitaria sanguinalis

text: Tromsoe herbarium specimen TROM_V_108171

text: TROM_V_108171 herbarium sample of Digitaria sanguinalis


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Elven, Reidar

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Melica uniflora

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Melica uniflora

text: Tromsoe herbarium specimen TROM_V_108776

characteristic: source material identifiers

text: TROM_V_108776 herbarium sample of Melica uniflora

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Anderssen, Harald

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Poa laxa subsp. flexuosa

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Poa laxa subsp. flexuosa

text: Tromsoe herbarium specimen TROM_V_109042

text: TROM_V_109042 herbarium sample of Poa laxa subsp. flexuosa


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Blom, Carl

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Sweden

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Hordeum comosum

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Hordeum comosum

text: Tromsoe herbarium specimen TROM_V_109900

text: TROM_V_109900 herbarium sample of Hordeum comosum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Oinonen, V.J.

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Finland

text: genome skimming

text: herbarium sample

text: Agropyron cristatum

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Agropyron cristatum

text: Tromsoe herbarium specimen TROM_V_109902

characteristic: source material identifiers

text: TROM_V_109902 herbarium sample of Agropyron cristatum

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Alm, Torbjorn; Often, Anders

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Alchemilla heptagona

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Alchemilla heptagona

text: Tromsoe herbarium specimen TROM_V_111092

text: TROM_V_111092 herbarium sample of Alchemilla heptagona


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Alm, Torbjorn; Gamst, Unni Bjerke

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Honckenya peploides

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Honckenya peploides

text: Tromsoe herbarium specimen TROM_V_111175

text: TROM_V_111175 herbarium sample of Honckenya peploides


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Pedersen, P.; Lojtnant, Bent; Jeppesen, Stig

characteristic: collection date

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Germany

text: genome skimming

text: herbarium sample

text: Scleranthus perennis

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Scleranthus perennis

text: Tromsoe herbarium specimen TROM_V_112194

characteristic: source material identifiers

text: TROM_V_112194 herbarium sample of Scleranthus perennis

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Jacobsen, Niels

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Cerastium semidecandrum

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Cerastium semidecandrum

text: Tromsoe herbarium specimen TROM_V_112459

text: TROM_V_112459 herbarium sample of Cerastium semidecandrum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Knutz, Harald

characteristic: collected by

text: Spanish catchfly

characteristic: common name

text: terrestrial biome

text: natural environment

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Silene otites

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Silene otites

text: Tromsoe herbarium specimen TROM_V_112883

text: TROM_V_112883 herbarium sample of Silene otites


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Saetra, Hartvig

characteristic: collection date

text: night-flowering catchfly

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Finland

text: genome skimming

text: herbarium sample

text: Silene noctiflora

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Silene noctiflora

text: Tromsoe herbarium specimen TROM_V_112905

characteristic: source material identifiers

text: TROM_V_112905 herbarium sample of Silene noctiflora

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Nettelbladt, Mats

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Selinum carvifolia

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Selinum carvifolia

text: Tromsoe herbarium specimen TROM_V_113369

text: TROM_V_113369 herbarium sample of Selinum carvifolia


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Morkved, Brynhild

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Koenigia divaricata

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Koenigia divaricata

text: Tromsoe herbarium specimen TROM_V_1140001

text: TROM_V_1140001 herbarium sample of Aconogonon divaricatum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Nordstroem, Asel

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Finland

text: genome skimming

text: herbarium sample

text: Cochlearia danica

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Cochlearia danica

text: Tromsoe herbarium specimen TROM_V_114360

characteristic: source material identifiers

text: TROM_V_114360 herbarium sample of Cochlearia danica

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Lauritz Holm-Nielsen, Benjamin Ollgaard, Stig Jeppesen

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Crambe maritima

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Crambe maritima

text: Tromsoe herbarium specimen TROM_V_114436

text: TROM_V_114436 herbarium sample of Crambe maritima


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Knutz, Harald

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Cardamine impatiens

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Cardamine impatiens

text: Tromsoe herbarium specimen TROM_V_114495

text: TROM_V_114495 herbarium sample of Cardamine impatiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Elven, Reidar

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Sweden

text: genome skimming

text: herbarium sample

text: Ilex aquifolium

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Ilex aquifolium

text: Tromsoe herbarium specimen TROM_V_114556

characteristic: source material identifiers

text: TROM_V_114556 herbarium sample of Ilex aquifolium

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Knutz, Harald

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Hydrocotyle vulgaris

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Hydrocotyle vulgaris

text: Tromsoe herbarium specimen TROM_V_114814

text: TROM_V_114814 herbarium sample of Hydrocotyle vulgaris


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Jacobsen, Niels

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Hedera helix

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Hedera helix

text: Tromsoe herbarium specimen TROM_V_114905

text: TROM_V_114905 herbarium sample of Hedera helix


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Lanes, Kristian

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Lamium amplexicaule

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Lamium amplexicaule

text: Tromsoe herbarium specimen TROM_V_115260

characteristic: source material identifiers

text: TROM_V_115260 herbarium sample of Lamium amplexicaule

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Elven, Reidar

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Lysimachia nemorum

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Lysimachia nemorum

text: Tromsoe herbarium specimen TROM_V_115446

text: TROM_V_115446 herbarium sample of Lysimachia nemorum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Alm, Torbjorn; Gamst, Unni Bjerke

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Alchemilla glaucescens

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Alchemilla glaucescens

text: Tromsoe herbarium specimen TROM_V_115480

text: TROM_V_115480 herbarium sample of Alchemilla glaucescens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Lojtnant, Bent ; Pedersen, P.

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

text: Leonurus cardiaca

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Leonurus cardiaca

text: Tromsoe herbarium specimen TROM_V_115614

characteristic: source material identifiers

text: TROM_V_115614 herbarium sample of Leonurus cardiaca

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Elven, Reidar

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Denmark

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Mentha aquatica

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Mentha aquatica

text: Tromsoe herbarium specimen TROM_V_116005

text: TROM_V_116005 herbarium sample of Mentha aquatica


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Engelskjon, Torstein

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Carex acutiformis

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Carex acutiformis

text: Tromsoe herbarium specimen TROM_V_12114

text: TROM_V_12114 herbarium sample of Carex acutiformis


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Elven, Reidar

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Sweden

text: genome skimming

text: herbarium sample

text: Carex tomentosa

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Carex tomentosa

text: Tromsoe herbarium specimen TROM_V_123988

characteristic: source material identifiers

text: TROM_V_123988 herbarium sample of Carex tomentosa

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Sortland, Andy; Hoyen, Gerd

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Carex panicea

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Carex panicea

text: Tromsoe herbarium specimen TROM_V_124143

text: TROM_V_124143 herbarium sample of Carex panicea


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Engelskjon, Torstein; Steilnes, Anne

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Puccinellia maritima

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Puccinellia maritima

text: Tromsoe herbarium specimen TROM_V_12470

text: TROM_V_12470 herbarium sample of Puccinellia maritima


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Edvardsen, Hanne; Moe, Bjorn

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Scheuchzeria palustris

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Scheuchzeria palustris

text: Tromsoe herbarium specimen TROM_V_126379

characteristic: source material identifiers

text: TROM_V_126379 herbarium sample of Scheuchzeria palustris

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Alm, Torbjorn

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Menyanthes trifoliata

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Menyanthes trifoliata

text: Tromsoe herbarium specimen TROM_V_126492

text: TROM_V_126492 herbarium sample of Menyanthes trifoliata


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Alm, Torbjorn

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Atriplex lapponica

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Atriplex lapponica

text: Tromsoe herbarium specimen TROM_V_126499

text: TROM_V_126499 herbarium sample of Atriplex lapponica


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Alm, Torbjorn; Gamst, Unni Bjerke

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Campanula glomerata

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Campanula glomerata

text: Tromsoe herbarium specimen TROM_V_126635

characteristic: source material identifiers

text: TROM_V_126635 herbarium sample of Campanula glomerata

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Alm, Torbjorn

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Barbarea vulgaris subsp. arcuata

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Barbarea vulgaris subsp. arcuata

text: Tromsoe herbarium specimen TROM_V_126745

text: TROM_V_126745 herbarium sample of Barbarea vulgaris var. arcuata


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Alm, Torbjorn; Gamst, Unni Bjerke

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Crepis paludosa

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Crepis paludosa

text: Tromsoe herbarium specimen TROM_V_126860

text: TROM_V_126860 herbarium sample of Crepis paludosa


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Alm, Torbjorn; Piirainen, Mikko

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Norway

text: genome skimming

text: herbarium sample

text: Phyteuma spicatum

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Phyteuma spicatum

text: Tromsoe herbarium specimen TROM_V_127166

characteristic: source material identifiers

text: TROM_V_127166 herbarium sample of Phyteuma spicatum

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Alm, Torbjorn

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Lathyrus linifolius

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Lathyrus linifolius

text: Tromsoe herbarium specimen TROM_V_127802

text: TROM_V_127802 herbarium sample of Lathyrus linifolius


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Lauritz Holm-Nielsen,

characteristic: collection date

text: terrestrial biome

text: natural environment

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

characteristic: isolation and growth condition

text: Epipactis palustris

characteristic: organism

text: herbarium sample

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf (PO_0025034)

text: Epipactis palustris

text: Tromsoe herbarium specimen TROM_V_128128

text: TROM_V_128128 herbarium sample of Epipactis palustris


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Tromsoe University Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Nielsen, Ivan; Lojtnant, Bent; Esbensen, Westy

text: terrestrial biome

characteristic: environment (biome)

text: natural environment

characteristic: environment (feature)

text: plant matter

text: Denmark

text: genome skimming

text: herbarium sample

text: Hydrocharis morsus-ranae

text: herbarium sample

text: soil

text: leaf (PO_0025034)

characteristic: plant structure

text: Hydrocharis morsus-ranae

text: Tromsoe herbarium specimen TROM_V_128230

characteristic: source material identifiers

text: TROM_V_128230 herbarium sample of Hydrocharis morsus-ranae

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Tromsoe University Museum

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Engelskjon, Torstein

characteristic: collected by

text: terrestrial biome

text: natural environment

text: plant matter

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: genome skimming

characteristic: investigation type

text: herbarium sample

text: Vahlodea atropurpurea

text: herbarium sample

text: soil

text: leaf (PO_0025034)

text: Vahlodea atropurpurea

text: Tromsoe herbarium specimen TROM_V_128883

text: TROM_V_128883 herbarium sample of Vahlodea atropurpurea