text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B10

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B4

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_B7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_B8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_B9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C20

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_C7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_C9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_C8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D4

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_D7

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_D8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_D9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E23

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_E7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_E8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_E9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_F7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_F8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_F9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G10

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G3

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_G7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_G8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_G9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_H7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_H9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_H8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625051266:FCA_P17_female_leg_I10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625051266:FCA_P17_female_leg_I11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: leg

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281625051266:FCA_P17_female_leg_I12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: leg

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title