text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_E21

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_E22

unit: day

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_E23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_E24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_E2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_E3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_E4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_E5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_E6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_E7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_E8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_E9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F12

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F16

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F18

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F20

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_F7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_F8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_F9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G12

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G17

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G18

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G20

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G2

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_G7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_G8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_G9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H11

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H12

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H14

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H19

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H17

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H1

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H20

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H22

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H2

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: BCM

text: public

text: E-MTAB-106281624982104:FCA_P3_female_gut_H5

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281624982104:FCA_P3_female_gut_H6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281624982104:FCA_P3_female_gut_H7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: title


text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Alderley Park

text: Samples prepared at Lighthouse Lab in Alderley Park by Jacquelyn Wynn, Mairead Hyland, The Lighthouse Lab in Alderley Park and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: England

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC

text: Lighthouse Laboratory Plymouth

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human

characteristic: organism


characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: Samples prepared at Lighthouse Lab in Milton Keynes by The Lighthouse Lab in Milton Keynes and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human


text: KYUNG HEE UNIVERSITY

text: public

characteristic: INSDC status

tag: Namespace:KYUNG HEE UNIVERSITY

unit: week

characteristic: age

text: ArrayExpress

text: Japanese rice

characteristic: common name

text: Chilbo

characteristic: cultivar

text: Protocols: After sufficient growth, plants were divided into two batches, where one batch was treated with 250 mM NaCl and other batch consists of control, which was remained in soil trays placed in water. Salt-treated samples were washed with 250 mM NaCl prior to sample collection. Seedlings and root morphology were analyzed, and sample collection was done for each batch from four independent physiological experiments, which was carried out at an interval of 2 weeks. Seeds of japonica rice cv. Chilbo were sterilized with sodium hypochlorite and germinated in MSO media in an incubator (14-h light/10-h dark, 28 °C (day)/22 °C (night), humidity 80%; Yonghwa Science, Daegu, Korea). After 1 week of germination in the incubator, seedlings were transferred and grown in plastic tray containing commercial soil supplemented with water for a period of 4 weeks under growth chamber condition. Total RNA was isolated using RNAiso kits (Takara Bio, Shiga, Japan) and first-strand cDNA was synthesized with MMLV Reverse Transcriptase (Promega, WI, USA) and the oligo(dT) 15 primer. Synthesized cDNAs were amplified using a SYBR Premix Ex Taq (Takara) before qRT-PCR was performed on a Rotor-Gene Q instrument system (Qiagen, Hiden, Germany). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, tagmentation combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.

text: seedling

characteristic: developmental stage

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: root

characteristic: organism part

text: Oryza sativa Japonica Group


text: KYUNG HEE UNIVERSITY

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Japanese rice

text: Chilbo

text: seedling

text: salt treatment

characteristic: environmental stress

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: root

text: Oryza sativa Japonica Group

characteristic: title


E-MTAB-10655:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10655:Sample 1

tag: Namespace:Dept. Biology, University or Rome Tor Vergata

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: macrophage

characteristic: cell type

text: house mouse

text: Protocols: Cells were detached by scraping in cell culture medium, collected through centrifugation and stored at -80 prior to RNA etraction Total RNA was isolated with TRIzol Reagent (Invitrogen) and purified using the RNeasy mini kit protocol (Qiagen), according to the manufacturer’s instructions. The libraries were prepared with a TruSeq Stranded mRNA (Illumina) Library Prep kit according to the manufacturer’s instructions

characteristic: description

text: leukemia

characteristic: disease

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: Sample 1


E-MTAB-10655:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Dept. Biology, University or Rome Tor Vergata

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10655:Sample 2

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood

text: Mus musculus

text: Sample 2


E-MTAB-10655:Sample 3

text: public

characteristic: SRA accession

text: E-MTAB-10655:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: common name

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: Sample 3


E-MTAB-10655:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10655:Sample 4

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: Sample 4

characteristic: title


E-MTAB-10655:Sample 10

text: public

text: E-MTAB-10655:Sample 10

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: macrophage

characteristic: cell type

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: brown adipocyte extracellular vesicle

characteristic: stimulus

text: Sample 10


E-MTAB-10655:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10655:Sample 11

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: description

text: leukemia

characteristic: disease

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: brown adipocyte extracellular vesicle

text: Sample 11


E-MTAB-10655:Sample 12

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10655:Sample 12

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: brown adipocyte extracellular vesicle

text: Sample 12


E-MTAB-10655:Sample 9

text: public

characteristic: SRA accession

text: E-MTAB-10655:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: common name

text: leukemia

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood

text: Mus musculus

text: brown adipocyte extracellular vesicle

text: Sample 9


E-MTAB-10655:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10655:Sample 13

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: brown adipocyte extracellular vesicle and lipopolysaccharide

text: Sample 13

characteristic: title


E-MTAB-10655:Sample 14

text: public

text: E-MTAB-10655:Sample 14

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: macrophage

characteristic: cell type

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

characteristic: stimulus

text: Sample 14


E-MTAB-10655:Sample 15

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10655:Sample 15

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: description

text: leukemia

characteristic: disease

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: Sample 15


E-MTAB-10655:Sample 16

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10655:Sample 16

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: Sample 16


E-MTAB-10655:Sample 5

text: public

characteristic: SRA accession

text: E-MTAB-10655:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: common name

text: leukemia

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood

text: Mus musculus

text: lipopolysaccharide

text: Sample 5


E-MTAB-10655:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10655:Sample 6

text: ArrayExpress

text: macrophage

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

characteristic: organism part

text: Mus musculus

text: lipopolysaccharide

text: Sample 6

characteristic: title


E-MTAB-10655:Sample 7

text: public

text: E-MTAB-10655:Sample 7

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: macrophage

characteristic: cell type

text: house mouse

text: leukemia

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: lipopolysaccharide

characteristic: stimulus

text: Sample 7


E-MTAB-10655:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10655:Sample 8

text: ArrayExpress

text: macrophage

text: house mouse

characteristic: description

text: leukemia

characteristic: disease

text: Mus musculus

text: Mus musculus

text: blood

text: Mus musculus

text: lipopolysaccharide

text: Sample 8


text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_H8

characteristic: age

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I10

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J17

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I11

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_H9

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J18

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I12

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I13

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I14

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J19

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I16

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I17

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I18

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I19

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J1

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I1

characteristic: age

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J16

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J21

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I20

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I21

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I22

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I23

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I5

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I3

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J23

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_I7

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_I9

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_I8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J2

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J10

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J4

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J11

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J5

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J7

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J9

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K10

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_K11

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K13

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K14

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J22

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J12

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_J15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_J14

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_J13

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_K16

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K17

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K19

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_K18

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K1

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_K21

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K22

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K23

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625046546:FCA_P3_female_gut_K24

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: description

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

text: Drosophila melanogaster

text: female

text: OK


characteristic: ENA-CHECKLIST

text: BCM

text: public

text: E-MTAB-106281625046546:FCA_P3_female_gut_K2

text: ArrayExpress

text: fruit fly

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: gut

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625046546:FCA_P3_female_gut_K5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: gut

characteristic: organism part

text: pass

text: Drosophila melanogaster

text: female

text: OK

characteristic: title