text: UNIVERSIDAD DE VALENCIA

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Servicio de Microbiología. Hospital Universitario y Politécnico La Fe

characteristic: collecting institution

text: Spain

characteristic: geographic location (country and/or sea)

text: Valencia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSIDAD DE VALENCIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:UNIVERSIDAD DE VALENCIA

characteristic: Submitter Id

text: Servicio de Microbiología. Hospital General Universitario de Alicante

characteristic: collection date

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Alicante

text: Human

text: Homo sapiens

characteristic: host subject id

characteristic: isolate

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

characteristic: title


text: UNIVERSIDAD DE VALENCIA

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Servicio de Microbiología. Hospital Clinico Universitario de Castellón

characteristic: collecting institution

text: Spain

characteristic: geographic location (country and/or sea)

text: Castellón

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSIDAD DE VALENCIA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Spain

text: Valencia

text: Human

text: Homo sapiens

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: WAGENINGEN UNIVERSITY

text: public

tag: Namespace:WAGENINGEN UNIVERSITY

characteristic: Submitter Id

characteristic: description

text: bovine gut metagenome

text: bovine gut metagenome


text: WAGENINGEN UNIVERSITY

text: public

text: RF

text: bovine gut metagenome

characteristic: organism

text: bovine gut metagenome

characteristic: title


text: WAGENINGEN UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Mock

text: bovine gut metagenome

text: bovine gut metagenome


text: TECHNICAL UNIVERSITY MUNICH

text: public

tag: Namespace:TECHNICAL UNIVERSITY MUNICH

characteristic: collection date

text: Wastewater sand filter amplicon

text: wastewater

characteristic: environment (biome)

text: wastewater treatment plants

characteristic: environment (feature)

text: Sand

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: wastewater metagenome

text: TOrCs biotransformation

characteristic: project name

text: wastewater metagenome

text: Illumina

characteristic: sequencing method

text: Sand filter amplicon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TECHNICAL UNIVERSITY MUNICH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Drinking water sand filter amplicon

characteristic: description

text: wastewater

text: wastewater treatment plants

text: Sand

text: Germany

text: metagenome

text: microbial mat/biofilm

text: drinking water metagenome

characteristic: organism

text: TOrCs biotransformation

text: drinking water metagenome

text: Illumina

text: Sand filter amplicon


text: University of Iceland

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Iceland

characteristic: collection date

text: 3 x 0-5 cm pooled

characteristic: composite design/sieving (if any)

text: Sub-arctic tundra

characteristic: environment (biome)

text: Highland

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

unit: mm

characteristic: mean annual and seasonal precipitation

unit: ºC

characteristic: mean annual and seasonal temperature

text: soil metagenome

text: Shotgun metagenome sequences soil microbial diversity and Empetrum nigrum

text: Soil core diameter 2 cm

characteristic: sample collection device or method

unit: g

characteristic: sample weight for DNA extraction

text: soil metagenome

text: Shotgun metagenomic

text: soil

characteristic: soil environmental package

text: Brown Andosol

characteristic: soil taxonomic/local classification

text: Grass and Empetrum nigrum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Institute of Human Genetics Polish Academy of Sciences

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded mRNA and Total RNA Library Prep Kit. Standard fragmentation conditions were used (94’C for 8 minutes; referred to as P1).

characteristic: description

text: child

characteristic: developmental stage

text: childhood T acute lymphoblastic leukemia

characteristic: disease

text: 94C for 8 minutes

characteristic: fragmentation protocol

characteristic: individual

text: Illumina TruSeq Stranded mRNA

characteristic: library protocol

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Human Genetics Polish Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: human

text: Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded mRNA and Total RNA Library Prep Kit. Modified fragmentation conditions were used (90'C for 2 minutes; referred to as P2)

text: child

text: 90C for 2 minutes

text: Illumina TruSeq Stranded mRNA

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat. Standard fragmentation conditions were used (94’C for 8 minutes; referred to as P1).

characteristic: description

text: child

characteristic: developmental stage

text: 94C for 8 minutes

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male


text: public

text: ArrayExpress

text: human

text: Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat. Modified fragmentation conditions were used (90'C for 2 minutes; referred to as P2).

text: child

characteristic: disease

text: 90C for 2 minutes

characteristic: fragmentation protocol

characteristic: individual

characteristic: library protocol

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: human

text: child

text: 94C for 8 minutes

text: Illumina TruSeq Stranded mRNA

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: child

characteristic: developmental stage

text: 90C for 2 minutes

text: Illumina TruSeq Stranded mRNA

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female


text: public

text: ArrayExpress

text: human

text: child

characteristic: disease

text: 94C for 8 minutes

characteristic: fragmentation protocol

characteristic: individual

text: TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat

characteristic: library protocol

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: human

text: child

text: 90C for 2 minutes

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: child

characteristic: developmental stage

text: 94C for 8 minutes

text: Illumina TruSeq Stranded mRNA

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male


text: public

text: ArrayExpress

text: human

text: child

characteristic: disease

text: 94C for 8 minutes

characteristic: fragmentation protocol

characteristic: individual

characteristic: library protocol

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ArrayExpress

text: human

text: child

text: 90C for 2 minutes

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Universite Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette cedex, France

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: MA lines obtained by classical plating and microfluidic approaches were conserved in a frozen stock and streaked on YPD medium for 48 hours at 30°C. In addition, the parental strains were also included in genome sequencing analysis. Colonies were then inoculated from the solid medium onto 10mL of liquid YPD overnight on a 30°C 180 rpm shaking incubator until saturation. Cells were then pelleted and resuspended in 200 μL of a buffer containing 10mM Tris-HCl pH 8.0, 1mM EDTA, 100 mM NaCl, 1% SDS, 2% Triton X100, for DNA extraction with glass beads (Sigma Aldrich) and Phenol:Chloroform:Isoamyl Alcohol (25:24:1) (Sigma-Aldrich) and vortexed at room temperature for 10 minutes. 200μL of Tris-EDTA (Sigma-Aldrich) buffer was added and this was centrifuged at 1300rpm for 5 minutes at room temperature. The supernatant was then precipitated with twice the volume of cold absolute Ethanol (VWR Chemicals) and 0.1 volume of 3M NaOAc (Sigma Aldrich). The solution was precipitated for 20 minutes at -80°C, centrifuged at 4°C and the pellet was washed with cold Ethanol 70% (VWR Chemicals) and then resuspended in water. The solution was treated with RNAse at 50μg/mL (Thermofisher Scientific) at 37°C for 1hour. The solution was precipitated with Ethanol for 20 minutes at -80°C, centrifuged at 4°C and the pellet was washed in cold Ethanol 70% (VWR Chemicals) and then resuspended in water. DNA was quantified using Nanodrop (Thermoscientific) and genomic DNA samples from 4 parallel MA lines for each strain were merged in equal quantities for library preparation. DNA was then sonicated with Covaris sonicator using the program of a Duty Factor of 10%, Peak incident power 175W, Cycles per burst 200 and a time of 180 seconds. Library preparation for genomic DNA was performed on IP-Star system using MicroPlex Library Preparation Kit v2 (Diagenode), following manufacturer's instructions.

characteristic: description

text: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1 ung1

characteristic: genotype

text: no mutation accumulation

characteristic: growth condition

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

characteristic: strain


text: Universite Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette cedex, France

text: public

text: E-MTAB-10259:classical_series1_UNG1D_B100

text: ArrayExpress

text: Manual 100 bottlenecks

text: Saccharomyces cerevisiae S288C

characteristic: organism

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10259:classical_series1_UNG1D_B50

text: ArrayExpress

text: Manual 50 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10259:microfluidic_series5_UNG1D_B100

text: ArrayExpress

text: Microfluidics 100 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C


characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10259:microfluidic_series5_UNG1D_B150

text: ArrayExpress

text: Microfluidics 150 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C


characteristic: External Id

text: public

text: E-MTAB-10259:microfluidic_series5_UNG1D_B50

text: ArrayExpress

text: Microfluidics 50 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C


characteristic: INSDC center name

text: public

text: ArrayExpress

text: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1

text: no mutation accumulation

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


characteristic: INSDC first public

text: public

text: ArrayExpress

text: Manual 150 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

characteristic: SRA accession

text: ArrayExpress

text: Manual 50 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: ArrayExpress

text: Manual 100 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT

characteristic: title


characteristic: INSDC last update

text: public

text: E-MTAB-10259:microfluidic_series1_YPH_B100

text: ArrayExpress

text: Microfluidics 100 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

characteristic: INSDC status

text: E-MTAB-10259:microfluidic_series2_YPH_B100

text: ArrayExpress

text: Microfluidics 100 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series2_YPH_B150

characteristic: Submitter Id

text: ArrayExpress

text: Microfluidics 150 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series2_YPH_B50

text: ArrayExpress

characteristic: broker name

text: Microfluidics 50 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series3_YPH_B100

text: ArrayExpress

characteristic: description

text: Microfluidics 100 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series3_YPH_B50

text: ArrayExpress

characteristic: genotype

text: Microfluidics 50 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series4_YPH_B100

text: ArrayExpress

text: Microfluidics 100 bottlenecks

characteristic: growth condition

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


text: public

text: E-MTAB-10259:microfluidic_series4_YPH_B150

text: ArrayExpress

text: Microfluidics 150 bottlenecks

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT

characteristic: strain


text: public

text: E-MTAB-10259:microfluidic_series4_YPH_B50

text: ArrayExpress

text: Protocols: MA lines obtained by classical plating and microfluidic approaches were conserved in a frozen stock and streaked on YPD medium for 48 hours at 30°C. In addition, the parental strains were also included in genome sequencing analysis. Colonies were then inoculated from the solid medium onto 10mL of liquid YPD overnight on a 30°C 180 rpm shaking incubator until saturation. Cells were then pelleted and resuspended in 200 μL of a buffer containing 10mM Tris-HCl pH 8.0, 1mM EDTA, 100 mM NaCl, 1% SDS, 2% Triton X100, for DNA extraction with glass beads (Sigma Aldrich) and Phenol:Chloroform:Isoamyl Alcohol (25:24:1) (Sigma-Aldrich) and vortexed at room temperature for 10 minutes. 200μL of Tris-EDTA (Sigma-Aldrich) buffer was added and this was centrifuged at 1300rpm for 5 minutes at room temperature. The supernatant was then precipitated with twice the volume of cold absolute Ethanol (VWR Chemicals) and 0.1 volume of 3M NaOAc (Sigma Aldrich). The solution was precipitated for 20 minutes at -80°C, centrifuged at 4°C and the pellet was washed with cold Ethanol 70% (VWR Chemicals) and then resuspended in water. The solution was treated with RNAse at 50μg/mL (Thermofisher Scientific) at 37°C for 1hour. The solution was precipitated with Ethanol for 20 minutes at -80°C, centrifuged at 4°C and the pellet was washed in cold Ethanol 70% (VWR Chemicals) and then resuspended in water. DNA was quantified using Nanodrop (Thermoscientific) and genomic DNA samples from 4 parallel MA lines for each strain were merged in equal quantities for library preparation. DNA was then sonicated with Covaris sonicator using the program of a Duty Factor of 10%, Peak incident power 175W, Cycles per burst 200 and a time of 180 seconds. Library preparation for genomic DNA was performed on IP-Star system using MicroPlex Library Preparation Kit v2 (Diagenode), following manufacturer's instructions.

text: Microfluidics 50 bottlenecks

text: Saccharomyces cerevisiae S288C

characteristic: organism

text: Saccharomyces cerevisiae S288C

text: Saccharomyces cerevisiae S288C

text: WT


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Department of evolutionary biology and environmental studies

characteristic: collection date

text: This culture contains a mixed consortium of aquatic algae and bacteria

text: culture

characteristic: environment (biome)

text: aquatic culture

characteristic: environment (feature)

text: glass culture

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: LEEF

characteristic: project name

text: metagenome

text: Illumina Novaseq 6000

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of evolutionary biology and environmental studies

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: description

text: culture

text: aquatic culture

text: glass culture

text: Switzerland

text: metagenome

text: metagenome

text: LEEF

text: metagenome

text: Illumina Novaseq 6000

characteristic: title

text: water


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF BIRMINGHAM

text: public

tag: Namespace:UNIVERSITY OF BIRMINGHAM

text: ArrayExpress

characteristic: broker name

text: Protocols: Biological triplicate samples were taken at two hourly intervals between 6 to 18 hours incubation inclusive. 1ml samples of fermentation broth from 6 - 12 hour time-points, and 0.5ml samples from time-points thereafter, were mixed with 2 volumes of RNA protect reagent (Qiagen). Cells were collected by centrifugation and frozen at -70°C until processing. Pseudomonas NCIMB10586 was grown in shake-flasks with medium containing, per litre; 23g yeast extract, 6.5g NaH2PO4, 5g (NH4)2SO4 and 1g glucose. Flasks were incubated for 15 hours at 25°C and 200rpm, prior to then being used to inoculate final stage 10L fermenters (1% v/v). Fermentation medium consisted of, per litre; 138g glucose, 26g defatted soyflour, 13.9g yeast extract, 11.9g rape seed oil, 4.7g (NH4)2SO4 , 4.6g corn steep liquor, 3.6g NaH2PO4, 3.3g PPG antifoam and 1.5g MgSO4.7H2O. Vessels were adjusted to a pre-inoculation pH of 6.8 and then incubated at 22°C, with DO2 maintained at 20% via agitation and following a natural drop pH control at 5.4 using 70% NH4OH. Total RNA was isolated from frozen bacterial samples using the miRNeasy mini kit (Qiagen). RNA was subsequently treated with RNase-Free DNase (New England Biolabs) and re-purified using the RNeasy mini kit (Qiagen). The concentration and quality of RNA in each sample was determined using a 2100 Bioanalyzer (Agilent). 21 barcoded libraries were created. To produce these, 1 µg total RNA was treated with the Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) (Epicentre, now manufactured by Illumina). After treatment the samples were fragmented for 8 minutes. The Illumina protocol was then followed from First Strand Synthesis through to Ligation of Adapters, then enriched using 10 cycles of PCR.

text: batch cell culture

characteristic: growth condition

text: Pseudomonas synxantha

characteristic: organism

text: Pseudomonas synxantha

text: sample volume 1 mL

characteristic: protocol

characteristic: replicate

text: Pseudomonas synxantha

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BIRMINGHAM

text: public

text: ArrayExpress

text: batch cell culture

text: Pseudomonas synxantha

text: Pseudomonas synxantha

text: sample volume 0.5 mL

text: Pseudomonas synxantha


characteristic: External Id

text: HCMR-IMBBC

characteristic: INSDC center name

text: public

text: sample ASB1-LL12-1 with tissue calcification

characteristic: isolate

text: fish metagenome

text: fish metagenome

text: kindey

characteristic: tissue lib

text: kidneys with calcification

characteristic: tissue type


text: HCMR-IMBBC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: sample ASB10-OO10-4 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


characteristic: ENA-CHECKLIST

text: HCMR-IMBBC

text: public

text: sample ASB2-NN8-2 with tissue calcification

characteristic: description

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification

characteristic: title


text: HCMR-IMBBC

text: public

text: sample ASB3-NN8-3 with tissue calcification

text: fish metagenome

characteristic: organism

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB4-NN11-2 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB5-OO3-1 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB6-OO7-1 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB7-OO7-3 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB8-OO10-1 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample ASB9-OO10-3 with tissue calcification

text: fish metagenome

text: fish metagenome

text: kindey

text: kidneys with calcification


text: HCMR-IMBBC

text: public

text: sample KOK1-LL1-1 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK10-LL5-2 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK2-LL1-2 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK3-LL1-3 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK4-LL5-3 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK5-MM2-1 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK6-LL12-2 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK7-LL12-4 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK8-LL12-3 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: sample KOK9-LL5-1 with granulomas visible macroscopically or microscopically

text: fish metagenome

text: fish metagenome

text: kindey

text: SG-affected


text: HCMR-IMBBC

text: public

text: PCR negative control sample

text: fish metagenome

text: fish metagenome

text: blank sample

text: PCR negative control


text: HCMR-IMBBC

text: public

text: sample O-1-OO7-2 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-2-OO3-3 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-3-MM9-1 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-4-MM9-4 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-5-NN6-1 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-6-NN6-2 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-7-MM4-1 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: HCMR-IMBBC

text: public

text: sample O-8-MM9-3 that did not have granulomas

text: fish metagenome

text: fish metagenome

text: kindey

text: healthy with no visible granulomas


text: NORWEGIAN VETERINARY INSTITUTE

text: public

characteristic: collection date

text: Klebsiella pneumoniae from healthy broilers and turkeys in Norway

text: Norway

characteristic: geographic location

text: Cecum

characteristic: isolation source

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NORWEGIAN VETERINARY INSTITUTE

text: public

text: Norway

text: Cecum

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

type: Other BioSample


characteristic: INSDC center name

text: public

tag: Namespace:Chair of Proteomics and Bioanalytics - Technical University of Munich

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: C57BL/6N mice (16 weeks old, m/f) were sacrificed individually by CO2 asphyxiation. Afterward, animal were perfused with 50 mL of precooled phosphate buffered saline, supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche) and a mixture of phosphatase inhibitor cocktail 1, 2 and 3 (Sigma-Aldrich). Tissues and organs were dissected, snap-frozen in liquid nitrogen and stored at -80 °C. Frozen tissues were then pulverized on liquid nitrogen. cDNA libraries were prepared using the TruSeq Stranded mRNA Sample Preparation kit (Ilumina) according to the manufacturer instructions Total RNA was isolated using the RNeasy Mini Plus Kit (Qiagen), according to the manufacturer instructions. RNA was quantified (Nanodrop, Thermo Fisher Scientific) and quality checked with a Tape Station D1000 (Agilent Technologies). RIN values between 7 and 10 were accepted for further analysis.

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: adrenal gland

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Adrenal.gland


characteristic: ENA-CHECKLIST

text: Chair of Proteomics and Bioanalytics - Technical University of Munich

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: brown adipose tissue

text: Mus musculus

text: male

text: Brown.adipose.tissue

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: cerebellum

text: Mus musculus

text: male

text: Cerebellum


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: lymph node

text: Mus musculus

text: male

text: Cervical.lymph.node


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: duodenum

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Duodenum


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: epididymis

text: Mus musculus

text: male

text: Epididymis


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: esophagus

text: Mus musculus

text: male

text: Esophagus

characteristic: title


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: frontal lobe

text: Mus musculus

text: male

text: Frontal.lobe


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: heart

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: Heart


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: hindbrain

text: Mus musculus

text: male

characteristic: strain

text: Hindbrain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: hippocampus

text: Mus musculus

text: male

text: Hippocampus


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: male

text: Kidneys

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: male

text: Liver


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: male

characteristic: sex

text: Lung


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: occipital lobe

text: Mus musculus

text: male

characteristic: strain

text: Occipital.lobe


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: olfactory system

text: Mus musculus

text: male

text: Olfactory.bulb


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: ovary

text: Mus musculus

text: female

text: Ovaries

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus

text: male

text: Pancreas


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: prostate gland

text: Mus musculus

text: male

characteristic: sex

text: Prostate


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: minor salivary gland

text: Mus musculus

text: male

characteristic: strain

text: Salivary.gland


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skeletal muscle tissue

text: Mus musculus

text: male

text: Skeletal.muscle


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin epidermis

text: Mus musculus

text: male

text: Skin

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: Mus musculus

text: female

text: Small.intestine


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: spinal cord

text: Mus musculus

text: male

characteristic: sex

text: Spinal.cord


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: stomach

text: Mus musculus

text: female

characteristic: strain

text: Stomach


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: temporal lobe

text: Mus musculus

text: male

text: Temporal.lobe


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: testis

text: Mus musculus

text: male

text: Testes

characteristic: title


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: tongue

characteristic: organism part

text: Mus musculus

text: male

text: Tongue


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: urinary bladder

text: Mus musculus

text: male

characteristic: sex

text: Urinary.bladder


E-MTAB-10251:cl 12 + tet

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10251:cl 12 + tet

tag: Namespace:Zentrum fur Molekulare Biologie der Universitat Heidelberg

characteristic: Submitter Id

text: ArrayExpress

text: EATRO 1125

characteristic: cell line

text: tetracycline

characteristic: compound

text: Protocols: centrifugation MEM-Medium, 27°C CBF2 gene expression was induced by treatment with tetracycline (100ng/ml) for 24 hr. TRI Reagent NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

text: procyclic

text: Nagana

characteristic: disease

unit: nanogram per milliliter

characteristic: dose

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

text: tetracycline induced expression of CFB2

characteristic: phenotype

text: Trypanosoma brucei

characteristic: strain

text: cl 12 + tet


E-MTAB-10251:cl 12- tet

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Zentrum fur Molekulare Biologie der Universitat Heidelberg

characteristic: INSDC center name

text: public

text: E-MTAB-10251:cl 12- tet

text: ArrayExpress

characteristic: broker name

text: EATRO 1125

text: Protocols: centrifugation MEM-Medium, 27°C TRI Reagent NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

characteristic: description

text: procyclic

text: Nagana

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: whole organism

text: wild type

text: Trypanosoma brucei

text: cl 12- tet

characteristic: title


E-MTAB-10251:cl 13 + tet

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10251:cl 13 + tet

text: ArrayExpress

text: EATRO 1125

text: tetracycline

text: procyclic

characteristic: developmental stage

text: Nagana

characteristic: genotype

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

characteristic: organism part

text: tetracycline induced expression of CFB2

text: Trypanosoma brucei

text: cl 13 + tet


E-MTAB-10251:cl 13 -tet

text: public

characteristic: SRA accession

text: E-MTAB-10251:cl 13 -tet

characteristic: Submitter Id

text: ArrayExpress

text: EATRO 1125

characteristic: cell line

text: procyclic

text: Nagana

characteristic: disease

characteristic: dose

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

text: wild type

characteristic: phenotype

text: Trypanosoma brucei

text: cl 13 -tet


E-MTAB-10251:cl 2 +tet

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10251:cl 2 +tet

text: ArrayExpress

characteristic: broker name

text: EATRO 1125

text: tetracycline

characteristic: compound

text: procyclic

text: Nagana

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

text: tetracycline induced expression of CFB2

text: Trypanosoma brucei

characteristic: strain

text: cl 2 +tet


E-MTAB-10251:cl 2 - tet

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10251:cl 2 - tet

text: ArrayExpress

text: EATRO 1125

characteristic: description

text: procyclic

text: Nagana

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: whole organism

text: wild type

text: Trypanosoma brucei

text: cl 2 - tet

characteristic: title


E-MTAB-10251:cl 3 + tet

text: public

characteristic: SRA accession

text: E-MTAB-10251:cl 3 + tet

characteristic: Submitter Id

text: ArrayExpress

text: EATRO 1125

characteristic: cell line

text: tetracycline

text: procyclic

characteristic: developmental stage

text: Nagana

characteristic: disease

characteristic: genotype

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

characteristic: organism part

text: tetracycline induced expression of CFB2

text: Trypanosoma brucei

text: cl 3 + tet


E-MTAB-10251:cl 3 -tet

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10251:cl 3 -tet

text: ArrayExpress

characteristic: broker name

text: EATRO 1125

text: procyclic

text: Nagana

characteristic: dose

text: Trypanosoma brucei

text: Trypanosoma brucei

text: whole organism

text: wild type

characteristic: phenotype

text: Trypanosoma brucei

text: cl 3 -tet


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 50615:c211ae6f-cc33-4251-a2f6-8a25eb57d90c

tag: Namespace:Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

text: GFBIO

characteristic: collection date

text: FRAM mooring Fevi-34: microbial annual cycle 2016-17

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial community [PCO:1000004]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: water

characteristic: environmental package

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Fram Strait

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

characteristic: mooring

text: PowerWater

characteristic: nucleic acid extraction

text: seawater metagenome

text: FRAM

characteristic: project name

text: Remote Access Sampler

characteristic: sample collection device or method

characteristic: sample description

characteristic: sample material processing

text: seawater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 50614:c211ae6f-cc33-4251-a2f6-8a25eb57d90c

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial community [PCO:1000004]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: water

text: Arctic Ocean

text: Fram Strait

text: mimarks-survey

text: PowerWater

text: seawater metagenome

characteristic: organism

text: FRAM

text: Remote Access Sampler

text: seawater metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: 50613:c211ae6f-cc33-4251-a2f6-8a25eb57d90c

text: GFBIO

text: FRAM mooring F4-S-1: microbial annual cycle 2016-17

text: marine biome [ENVO:00000447]

text: microbial community [PCO:1000004]

text: sea water [ENVO:00002149]

text: water

characteristic: environmental package

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Fram Strait

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

characteristic: mooring

text: PowerWater

characteristic: nucleic acid extraction

text: seawater metagenome

text: FRAM

characteristic: project name

text: Remote Access Sampler

characteristic: sample collection device or method

characteristic: sample description

characteristic: sample material processing

text: seawater metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 50612:c211ae6f-cc33-4251-a2f6-8a25eb57d90c

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: FRAM mooring HG-IV-S-1: microbial annual cycle 2016-17

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial community [PCO:1000004]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: water

text: Arctic Ocean

text: Fram Strait

text: mimarks-survey

text: PowerWater

text: seawater metagenome

characteristic: organism

text: FRAM

text: Remote Access Sampler

text: seawater metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

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