characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ROBERT KOCH INSTITUTE

text: public

characteristic: INSDC status

text: DEU

text: Salmonella

text: Germany

text: Homo sapiens

text: not provided

text: Homo sapiens

characteristic: isolate

text: stool

characteristic: isolation source

text: gastrointestinal system

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

text: Kentucky

text: Salmonella enterica serovar Kentucky


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ROBERT KOCH INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: DEU

characteristic: SRA accession

tag: Namespace:ROBERT KOCH INSTITUTE

characteristic: Submitter Id

characteristic: collection date

text: Salmonella

characteristic: genus

text: Germany

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: stool

text: gastrointestinal system

text: Salmonella enterica subsp. enterica

characteristic: organism

text: Salmonella enterica subsp. enterica

text: Napoli

characteristic: serovar

text: Salmonella enterica serovar Napoli

characteristic: title

type: AMR curated data - CABBAGE

value: ceftazidime

value: broth dilution

value: mg/L

value: Salmonella enterica

value: meropenem

value: cefotaxime

value: nalidixic acid

value: ciprofloxacin

value: cefoxitin

value: trimethoprim-sulfamethoxazole

value: ampicillin


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ROBERT KOCH INSTITUTE

text: public

characteristic: INSDC status

text: DEU

text: Salmonella

text: Germany

text: Homo sapiens

text: not provided

text: Homo sapiens

characteristic: isolate

text: stool

characteristic: isolation source

text: gastrointestinal system

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

text: Anatum

text: Salmonella enterica serovar Anatum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ROBERT KOCH INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: DEU

text: Salmonella

text: Germany

text: Homo sapiens

text: not provided

text: Homo sapiens

text: Salmonella enterica subsp. enterica

text: Salmonella enterica subsp. enterica

text: Poole

text: Salmonella enterica serovar Poole


text: DNASense

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: metagenome

text: metagenome


E-MTAB-9751:Sample 1

text: public

text: E-MTAB-9751:Sample 1

text: embryonic day 13.5

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Dissected ventral telencephalon fixed in 4% PGA for 10 min Phenol-chloroform extraction Ilumina ChIP-Seq library standard protocol

characteristic: description

text: embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: ventral part of telencephalon

characteristic: organism part

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-9751:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: E-MTAB-9751:Sample 2

text: embryonic day 13.5

text: ArrayExpress

text: house mouse

text: embryo

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: ventral part of telencephalon

text: Mus musculus

text: mixed

text: Sample 2

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LBCMCP, UMR5088, CBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:LBCMCP, UMR5088, CBI

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: For ChIP: Formaldehyde was added to the culture medium at a final concentration of 1% and crosslinking was allowed to proceed for 15 min at room temperature. Reaction was stopped by adding glycine (0.125M final concentration) and incubation for 5 minutes at room temperature. Cells were washed twice with PBS and harvested by scraping. DIvA (AsiSI-ER-U20S) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with antibiotics, 10% FCS (InVitrogenInvitrogen) with 1 µg/mL puromycin (DIvA cells) at 37°C under a humidified atmosphere with 5% CO2. For AsiSI-dependent DSB induction, cells were treated with 300 nM OHT (Sigma; H7904) for 4h. For ATM inhibition, cells were pretreated for 1 h with 20μM KU-55933 (Sigma, SML1109) and during subsequent 4OHT treatment. For ATR inhibition, cells were pretreated for 1 h with 2μM ETP-46464 (Sigma, SML1321) and during subsequent 4OHTsiRNA transfection were performed using the 4D Nucleofector X (kit SE cell line) according to the manufacturer's instructions and subsequent treatment(s) were performed 48 h later. siRNA sequences: CTRL: CAUGUCAUGUGUCACAUCU SCC1: GGUGAAAAUGGCAUUACGG WAPL: CGGACUACCCUUAGCACAA. For CRISPR/Cas9-mediated DSB induction, sgRNA (AsiSI site position: “CGCCGCGATCGCGGAATGGA” or Position further within the TAD=\CRISPRsite\: “GGGCCAGTCGCGGCACTCGC”) were delivered in U2OS cells using the “nanoblades” technology which relies on direct cell transduction with a viral-derived particle containing the Cas9/sgRNA ribonucleoprotein (Mangeot et al, 2019). Cells were analyzed 24 h after transduction. For ChIP: Cell lysis was performed by incubation in cell lysis buffer (5 mM Pipes pH 8, 85 mM KCl, 0.5% IGEPAL® CA-630) followed by Dounce homogenization. Nuclei were harvested by centrifugation and incubated in nuclear lysis buffer: (50 mM Tris pH 8.1, 10 mM EDTA, 1% SDS) and sonicated 10 times for 10 s at a power setting of 5 and 50% duty cycle (Branson Sonifier 250). Samples were diluted 10 times in ChIP dilution buffer (16.7 mM Tris pH 8.1, 167 mM NaCl, 1.2 mM EDTA, 1.1% Triton X‐100, 0.01% SDS) and precleared using a mixture of protein A-agarose (Pierce) and protein G-sepharose (Sigma) previously blocked with BSA (Sigma). Precleared samples (200 μg) were incubated overnight at 4°C with indicated antibodies. Immune complexes were recovered by incubation with blocked protein A/protein G beads for 2 h at 4°C on a rotating wheel. Beads were washed once in dialysis buffer (50 mM Tris pH 8, 2 mM EDTA, 0.2% Sarkosyl), five times in wash buffer (100 mM Tris pH 8.8, 500 mM LiCl, 1% IGEPAL® CA-630, 1% NaDoc) and twice with TE (10mM Tris-HCl, pH 8, 1mM EDTA). Beads were resuspended in TE supplemented with 50µg/ml DNase-free RNase A (Abcam) and incubated for 30 minutes at 37°C. SDS (final concentration 0.5%) was added and crosslink was reversed by overnight incubation at 70°C. Following a 2 hours treatment with 200 µg/ml proteinase K (Roche) at 45°C, DNA was purified by phenol/chloroform extraction and recovered by ethanol precipitation in presence of 5 µg glycogen (Invitrogen). DNA pellet was resuspended in water. Prior to next-generation sequencing library preparation, samples from multiples ChIP experiments were pooled and sonicated for 5 cycles (30 seconds on, 30 seconds off, high setting) on a Bioruptor (Diagenode) then concentrated with a vacuum concentrator (Eppendorf). For the calibrated ChIP-seq of SCC1, we used a spike-in method as in Kojic et al (2018). Briefly, cross-linked cells were first lysed 10min at 4°C in 500 µL of the Lysis Buffer 1 (10mM Tris pH8, 10mM NaCl, 0.5% NP-40, Complete protease inhibitor (Sigma)) then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode) in the presence of 800 mg of sonication beads (20 cycles of 30 sec ON/30 sec OFF) to an average fragment size of 250pb. Prior to immunoprecipitation with SCC1 antibody, 20% of chromatin from mouse ES cells (40µg) was added to chromatin prepared from treated or untreated human DIvA cells (200µg). Antibodies : pATM (S1981): Abcam ab81292 (2µg) SCC1: Abcam ab992 (4µg) MDC1: Abcam ab11171 (3µg) For ChIP: Sequencing libraries were prepared from IP of SCC1 and pATM(S1981) and input samples using the NEBNext® Ultra™ II Library Prep Kit (NEB)for Illumina. MDC1 libraries were prepared at EMBL Genomics core facilities (Heidelberg, Germany) using NEBNext ChIP-Seq Library Prep kit (New England Biolabs).

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: CTCFmOHT


text: LBCMCP, UMR5088, CBI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_pOHTpATMi

text: ArrayExpress

text: human

text: AsiSI-ER

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_pOHTpATMi_release15min

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_pOHTpATMi_release1h

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

text: public

characteristic: INSDC status

text: E-MTAB-88511605094083:gammaH2AX_pOHTpATMi_release5min

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_pOHTpATRi

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_siCTRL_pOHT

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_siSCC1_pOHT

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:gammaH2AX_siWAPL_pOHT

text: ArrayExpress

text: human

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: Protocols: DIvA (AsiSI-ER-U20S) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with antibiotics, 10% FCS (InVitrogenInvitrogen) with 1 µg/mL puromycin (DIvA cells) at 37°C under a humidified atmosphere with 5% CO2. For AsiSI-dependent DSB induction, cells were treated with 300 nM OHT (Sigma; H7904) for 4h. For ATM inhibition, cells were pretreated for 1 h with 20μM KU-55933 (Sigma, SML1109) and during subsequent 4OHT treatment. For ATR inhibition, cells were pretreated for 1 h with 2μM ETP-46464 (Sigma, SML1321) and during subsequent 4OHTsiRNA transfection were performed using the 4D Nucleofector X (kit SE cell line) according to the manufacturer's instructions and subsequent treatment(s) were performed 48 h later. siRNA sequences: CTRL: CAUGUCAUGUGUCACAUCU SCC1: GGUGAAAAUGGCAUUACGG WAPL: CGGACUACCCUUAGCACAA. For CRISPR/Cas9-mediated DSB induction, sgRNA (AsiSI site position: “CGCCGCGATCGCGGAATGGA” or Position further within the TAD=\CRISPRsite\: “GGGCCAGTCGCGGCACTCGC”) were delivered in U2OS cells using the “nanoblades” technology which relies on direct cell transduction with a viral-derived particle containing the Cas9/sgRNA ribonucleoprotein (Mangeot et al, 2019). Cells were analyzed 24 h after transduction. Hi-C experiments were performed in DIvA cells using the Arima Hi-C kit (Arima Genomics) according to the manufacturer’s instructions. 1 million cells were used by condition. Briefly, cells were cross-linked with 2% formaldehyde for 10 min at RT, lysed and chromatin was digested with two different restriction enzymes included in the kit. Ends were filled-in in the presence of biotinylated nucleotides, followed by subsequent ligation. Ligated DNA was sonicated using the Covaris S220 to an average fragment size of 350 bp with the following parameters (Peak incident power: 140; Duty factor: 10%; Cycles per burst: 200; Treatment time: 70s). DNA was then subjected to a double-size selection to retain DNA fragments between 200 and 600 bp using Ampure XP beads (Beckman Coulter). Biotin-ligated DNA was precipitated with streptavidin-coupled magnetic beads (included in the kit). Hi-C library was prepared on beads using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext® Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima Hi-C kit.

text: AsiSI-ER

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: LBCMCP, UMR5088, CBI

characteristic: INSDC first public

text: public

text: E-MTAB-88511605094083:yeast_PDS5AID_IAA_gal0min

text: ArrayExpress

text: baker's yeast

text: Protocols: For the ChIP experiment in yeast, individual colonies of yFZ014 and yFZ016 were grown in YEP + 3% lactic acid (YEP-Lac) until log phase growth with a final cell concentration between 5×106 cells/ml and 8×106 cells/ml. Degradation of Pds5::9myc-AID in yFZ016 was induced by addition of auxin (indole-3-acetic acid (IAA) Sigma Aldrich # I3750) at a final concentration of 1 mM and confirmed by Western blotting. HO expression is induced by adding 2% galactose during 75min (final concentration) to the medium. For chromatin immunopreciptiation, 45 ml of culture was fixed and cross-linked with 1% formaldehyde for 10 min, after which 2.5 ml of 2.5 M glycine was added for 5 min to quench the reaction. Cells were pelleted and washed 3 times with 4°C TBS. Yeast cell walls were disrupted by beating the cells with 425-600 µm glass beads for 1 h in lysis buffer at 4°C. The lysate was sonicated for 2 min to obtain chromatin fragments of ~500 bp in length. Debris was then pelleted and discarded, and equal volume of lysate was immunoprecipitated using γ-H2A antibody for 1 h at 4°C, followed by addition of Protein-A agarose beads (Sigma-Aldrich #1719408001) for 1 h at 4°C. The immunoprecipitate was then washed twice in 140 mM NaCl lysis buffer, once with 0.5 M NaCl lysis buffer, once with 0.25 M LiCl wash buffer and once with TE. Crosslinking was reversed at 65°C overnight followed by proteinase K and glycogen addition for 2 h. Protein and nucleic acids were separated by phenol extraction. LiCl was added to a final concentration of 400 mM LiCl. DNA was precipitated using 99.5% EtOH. A second precipitation step was carried out using 75% EtOH and the DNA resuspended in TE. For ChIP: Sequencing libraries were prepared from IP of SCC1 and pATM(S1981) and input samples using the NEBNext® Ultra™ II Library Prep Kit (NEB)for Illumina. MDC1 libraries were prepared at EMBL Genomics core facilities (Heidelberg, Germany) using NEBNext ChIP-Seq Library Prep kit (New England Biolabs). Sequencing libraries were prepared and sequenced as for ChIP-seq in human cells.

text: FZ016 - HOcse2:;URA HOcse6::HPH TIR1::Leu2 Pds5-9*myc-AID::KAN

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: LBCMCP, UMR5088, CBI

characteristic: INSDC last update

text: public

text: E-MTAB-88511605094083:yeast_PDS5AID_IAA_gal75min

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: LBCMCP, UMR5088, CBI

text: public

text: E-MTAB-88511605094083:yeast_PDS5AID_NoIAA_gal0min

text: ArrayExpress

characteristic: cell line

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: LBCMCP, UMR5088, CBI

text: public

characteristic: SRA accession

text: E-MTAB-88511605094083:yeast_PDS5AID_NoIAA_gal75min

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: Quadram Institute Bioscience

text: public

tag: Namespace:Quadram Institute Bioscience

characteristic: Submitter Id

text: Quadram Institute Bioscience

characteristic: cell type

text: Dr Ben Kirkup

characteristic: collected by

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from Water Treated mouse 01

text: RNAseq

characteristic: investigation type

text: Mus musculus

text: Mus musculus

text: non-stranded paried end protocol

characteristic: sequencing method

text: F

characteristic: sex

text: Orthotopic Tumour

characteristic: tissue type

text: Water

characteristic: treatment

text: Breast Cancer


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: INSDC status

text: Quadram Institute Bioscience

characteristic: broker name

text: Dr Ben Kirkup

text: house mouse

characteristic: common name

text: C57 BL/6 PyMT-BO1 Tumour from Water Treated mouse 02

characteristic: description

text: RNAseq

text: Mus musculus

characteristic: organism

text: Mus musculus

text: non-stranded paried end protocol

text: F

text: Orthotopic Tumour

text: Water

text: Breast Cancer


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Quadram Institute Bioscience

characteristic: cell line

text: Dr Ben Kirkup

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from Water Treated mouse 03

text: RNAseq

text: Mus musculus

text: Mus musculus

text: non-stranded paried end protocol

text: F

text: Orthotopic Tumour

characteristic: title

text: Water

text: Breast Cancer


text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Quadram Institute Bioscience

characteristic: cell type

text: Dr Ben Kirkup

characteristic: collected by

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from ABX Treated mouse 01

text: RNAseq

characteristic: investigation type

text: Mus musculus

text: Mus musculus

text: non-stranded paried end protocol

characteristic: sequencing method

text: F

characteristic: sex

text: Orthotopic Tumour

text: VNMAA

text: Breast Cancer


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

text: public

characteristic: INSDC status

text: Quadram Institute Bioscience

characteristic: broker name

text: Dr Ben Kirkup

text: house mouse

characteristic: common name

text: C57 BL/6 PyMT-BO1 Tumour from ABX Treated mouse 02

text: RNAseq

text: Mus musculus

text: Mus musculus

text: non-stranded paried end protocol

text: F

text: Orthotopic Tumour

characteristic: tissue type

text: VNMAA

characteristic: treatment

text: Breast Cancer


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Quadram Institute Bioscience

text: Dr Ben Kirkup

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from ABX Treated mouse 03

characteristic: description

text: RNAseq

text: Mus musculus

characteristic: organism

text: Mus musculus

text: non-stranded paried end protocol

text: F

text: Orthotopic Tumour

text: VNMAA

text: Breast Cancer


text: TECHNICAL UNIVERSITY MUNICH

text: public

characteristic: SRA accession

tag: Namespace:TECHNICAL UNIVERSITY MUNICH

characteristic: Submitter Id

text: macrophages

characteristic: cell type

text: house mouse

text: Mus musculus

text: Mus musculus

text: male

characteristic: strain

text: Sham

characteristic: surgery

text: 6 days

text: heart

text: RNA-Seq of cardiac macrophages 6 days after pressure overload

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: TECHNICAL UNIVERSITY MUNICH

text: public

characteristic: INSDC status

text: macrophages

text: house mouse

text: Mus musculus

text: Mus musculus

text: male

characteristic: sex

text: TAC

text: 6 days

text: heart


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: bio material

characteristic: collection date

characteristic: collection time

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 85

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


characteristic: External Id

text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 86

characteristic: description

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

characteristic: Submitter Id

text: Atlantic salmon

text: Brain ID Number 87

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

characteristic: bio material

text: Atlantic salmon

text: Brain ID Number 88

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: collection date

characteristic: collection time

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 89

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: External Id

text: IOA

text: public

text: Atlantic salmon

text: Brain ID Number 90

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Atlantic salmon

text: Brain ID Number 97

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 98

characteristic: description

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

characteristic: Submitter Id

text: Atlantic salmon

text: Brain ID Number 99

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

text: Atlantic salmon

text: Brain ID Number 100

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


text: IOA

text: public

characteristic: bio material

characteristic: collection date

characteristic: collection time

text: Atlantic salmon

text: Brain ID Number 101

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: External Id

text: IOA

text: public

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 102

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Atlantic salmon

text: Brain ID Number 109

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


text: IOA

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 110

characteristic: description

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

text: Atlantic salmon

text: Brain ID Number 111

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Atlantic salmon

text: Brain ID Number 112

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: bio material

characteristic: collection date

text: Atlantic salmon

text: Brain ID Number 113

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


characteristic: External Id

text: IOA

text: public

characteristic: collection time

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 114

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Atlantic salmon

text: Brain ID Number 121

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 122

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

text: Atlantic salmon

text: Brain ID Number 123

characteristic: description

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

text: Atlantic salmon

text: Brain ID Number 124

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: Submitter Id

characteristic: bio material

characteristic: collection date

text: Atlantic salmon

text: Brain ID Number 125

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: External Id

text: IOA

text: public

characteristic: collection time

text: Atlantic salmon

text: Brain ID Number 126

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 133

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 134

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

text: Atlantic salmon

text: Brain ID Number 135

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

text: Atlantic salmon

text: Brain ID Number 136

characteristic: description

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: Submitter Id

characteristic: bio material

text: Atlantic salmon

text: Brain ID Number 137

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


characteristic: External Id

text: IOA

text: public

characteristic: collection date

characteristic: collection time

text: Atlantic salmon

text: Brain ID Number 138

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Atlantic salmon

text: Brain ID Number 145

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


text: IOA

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 146

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

text: Atlantic salmon

text: Brain ID Number 147

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

text: Atlantic salmon

text: Brain ID Number 148

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


text: IOA

text: public

characteristic: Submitter Id

characteristic: bio material

text: Atlantic salmon

text: Brain ID Number 149

characteristic: description

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: External Id

text: IOA

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characteristic: collection date

text: Atlantic salmon

text: Brain ID Number 150

text: fry

text: Salmo salar

characteristic: organism

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection time

text: Atlantic salmon

text: Brain ID Number 157

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: Atlantic salmon

text: Brain ID Number 158

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOA

text: public

text: Atlantic salmon

characteristic: common name

text: Brain ID Number 159

text: fry

text: Salmo salar

text: Salmo salar

text: brain


characteristic: ENA-LAST-UPDATE

text: IOA

text: public

characteristic: INSDC status

text: Atlantic salmon

text: Brain ID Number 160

text: fry

characteristic: dev stage

text: Salmo salar

text: Salmo salar

text: brain


text: IOA

text: public

characteristic: Submitter Id

characteristic: bio material

text: Atlantic salmon

text: Brain ID Number 161

text: fry

text: Salmo salar

text: Salmo salar

text: brain

characteristic: tissue type


characteristic: External Id

text: IOA

text: public

characteristic: collection date

text: Atlantic salmon

text: Brain ID Number 162

characteristic: description

text: fry

text: Salmo salar

text: Salmo salar

text: brain


text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:UNIVERSITY OF ST ANDREWS

text: upper sandur borehole 1(a-c)

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: ICEbac_U1 (pooled a-c)

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: upper sandur borehole 2a

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: title

text: water


text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection date

text: upper sandur borehole 2b

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

text: upper sandur borehole 2c

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

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text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

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characteristic: ENA-LAST-UPDATE

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text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: middle sandur borehole 1 (a-c)

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: ICEbac_M1 (pooled a-c)

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: middle sandur borehole 2a

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

text: middle sandur borehole 2c

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: lower sandur borehole 3a

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: lower sandur borehole 3b

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

text: lower sandur borehole 3c

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: river sample

text: Glacier forefield

text: aquifer

text: river water

text: Iceland

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: spring sample 1

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: spring water

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: ICEbac_spring 1

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

text: spring sample 2

text: Glacier forefield

text: aquifer

text: spring water

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: ICEbac_spring 2

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: proglacial lake outlet

text: Glacier forefield

text: aquifer

text: lake water

text: Iceland

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: glacier stream

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: stream water

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified bacterium

text: Virkisjökull sandur microbial community

text: unidentified bacterium

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

text: DNA extraction control

text: DNA extraction kit

text: filter

text: filter

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified bacterium

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified bacterium

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: upper sandur borehole 2a

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: upper sandur borehole 2b

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

text: middle sandur borehole 2a

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified archaeon

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: middle sandur borehole 2b

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: middle sandur borehole 2c

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

text: middle sandur borehole 3 (a-c)

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified archaeon

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: lower sandur borehole 1(a-c)

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: lower sandur borehole 2(a-c)

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

text: lower sandur borehole 3a

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified archaeon

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: lower sandur borehole 3b

text: Glacier forefield

text: aquifer

text: groundwater

text: Iceland

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: lower sandur borehole 3c

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: groundwater

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

text: spring sample 1

text: Glacier forefield

text: aquifer

text: spring water

text: Iceland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: unidentified archaeon

characteristic: organism

text: Virkisjökull sandur microbial community

characteristic: project name

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF ST ANDREWS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: spring sample 2

text: Glacier forefield

text: aquifer

text: spring water

text: Iceland

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF ST ANDREWS

text: public

characteristic: collection date

text: glacier stream

characteristic: description

text: Glacier forefield

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: stream water

characteristic: environment (material)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: mimarks-survey

text: unidentified archaeon

text: Virkisjökull sandur microbial community

text: unidentified archaeon

text: Illumina MiSeq amplicon

text: water


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UIO

text: public

unit: L

characteristic: amount or size of sample collected

characteristic: chlorophyll

text: Ocean

text: Open Ocean

text: plankton

text: Svalbard

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Arctic Ocean

characteristic: geographic location (region and locality)

text: eukaryote

characteristic: investigation type

text: KAPA HiFi HotStart ReadyMix 2x

characteristic: nucleic acid amplification

text: DNeasy Plant mini kit

characteristic: nucleic acid extraction

text: marine metagenome

characteristic: organism

text: F: 5'-CCAGCASCYGCGGTAATTCC-3', R: 5'-ACTTTCGTTCTTGATYRATGA-3' (Piredda et al. 2017)

characteristic: pcr primers

text: MicroPolar

characteristic: project name

unit: practical salinity unit

characteristic: salinity

text: Niskin bottle

characteristic: sample collection device or method

text: filtration

characteristic: sample material processing

unit: mL

characteristic: sample volume or weight for DNA extraction

text: marine metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: 18S rDNA

characteristic: target gene

characteristic: target subfragment

unit: ºC

characteristic: temperature

characteristic: total depth of water column

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UIO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: chlorophyll

characteristic: collection date

text: Ocean

characteristic: environment (biome)

text: Open Ocean

characteristic: environment (feature)

text: plankton

characteristic: environment (material)

text: Svalbard

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Arctic Ocean

characteristic: geographic location (region and locality)

text: eukaryote

text: KAPA HiFi HotStart ReadyMix 2x

text: DNeasy Plant mini kit

text: marine metagenome

text: MicroPolar

text: Net haul

text: filtration

text: marine metagenome

text: Illumina HiSeq

text: 18S rDNA

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Vertebrate Biology, CAS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Institute of Vertebrate Biology, CAS

text: Jacek M Szymura

characteristic: collected by

text: Fire-bellied toad

characteristic: common name

text: offspring of SAMEA7325035 and SAMEA7325115

characteristic: description

text: early metamorphsis

characteristic: dev stage

text: laboratory cross

characteristic: isolation source

text: Bombina bombina x Bombina variegata

characteristic: organism

text: Bombina bombina x Bombina variegata

text: f

characteristic: sex

text: F2 generation (family 6)


text: Institute of Vertebrate Biology, CAS

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Jacek M Szymura

characteristic: collection date

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: m

text: F2 generation (family 6)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute of Vertebrate Biology, CAS

text: public

text: Jacek M Szymura

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: (f)

text: F2 generation (family 6)

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Vertebrate Biology, CAS

characteristic: INSDC center name

text: public

text: Jacek M Szymura

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: (m)

text: F2 generation (family 6)


text: Institute of Vertebrate Biology, CAS

characteristic: INSDC first public

text: public

text: Jacek M Szymura

characteristic: collected by

text: Fire-bellied toad

characteristic: common name

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: F2 generation (family 6)


text: Institute of Vertebrate Biology, CAS

text: public

text: Jacek M Szymura

text: Fire-bellied toad

text: offspring of SAMEA7325116 and SAMEA7325115

characteristic: description

text: early metamorphsis

characteristic: dev stage

text: laboratory cross

characteristic: isolation source

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: f

text: F2 generation (family 7)


text: Institute of Vertebrate Biology, CAS

characteristic: INSDC last update

text: public

text: Jacek M Szymura

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

characteristic: organism

text: Bombina bombina x Bombina variegata

text: m

characteristic: sex

text: F2 generation (family 7)


text: Institute of Vertebrate Biology, CAS

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Jacek M Szymura

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: (m)

text: F2 generation (family 7)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute of Vertebrate Biology, CAS

text: public

text: Jacek M Szymura

characteristic: collection date

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: (f)

text: F2 generation (family 7)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Vertebrate Biology, CAS

text: public

text: Jacek M Szymura

text: Fire-bellied toad

text: early metamorphsis

text: laboratory cross

text: Bombina bombina x Bombina variegata

text: Bombina bombina x Bombina variegata

text: F2 generation (family 7)

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen

text: ArrayExpress

characteristic: broker name

text: 129 EpiSC

characteristic: cell line

text: epiblast cell

characteristic: cell type

text: house mouse

text: Protocols: EpiSCs were seeded at ~6 x 10^3 cells/cm2 in 6-well cell-culture plates (Corning, 3506) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015), and differentiated to pre-somitic mesoderm (PSM) as described below. Wild-type EpiSC lines were derived from E6.5 mouse embryos (129 background) and maintained in 6-well cell-culture plates (Corning, 3506) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015) in N2B27 medium (prepared as described in Ying Q.L. & Smith A.G., Methods Enzymol 365, 327-341 (2003)), supplemented with 20 ng/ml Activin A, 10 ng/ml bFGF and 1 µM XAV939 (EpiSC medium). Pbx1/Pbx2 double-knockout (Pbx1/2-DKO) EpiSC lines and lines carrying base-pair substitutions on the Msgn1 promoter that abolish PBX recruitment (pMsgn1-mut) were generated by CRISPR/Cas9. All differentiation was conducted in serum-free, feeder-free and monolayer conditions in chemically-defined N2B27-based media. Culture conditions followed a modified protocol from Loh K.M. et al., Cell 166, 451-467 (2016). EpiSCs were seeded at ~6 x 10^3 cells/cm2 in 6-well cell-culture plates pre-coated with 15 µg/ml human Fibronectin, and cultured in EpiSC medium for 24 h. EpiSCs were first differentiated towards anterior primitive streak (APS) using 30 ng/ml Activin A, 4 µM CHIR-99021, 20 ng/ml bFGF and 100 nM PIK-90 (APS medium) for 12 h, and then to pre-somitic mesoderm (PSM) using 1 µM A-83-01, 3 µM CHIR-99021, 250 nM LDN193189 and 20 ng/ml bFGF (PSM medium) for 36 h. At desired time-points (12 hours and 24 hours of PSM differentiation), cells were lysed and total RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106) according to the manufacturer’s instructions. RNA concentration was quantified by NanoDrop (Thermo Fisher Scientific) and quality was verified with a Fragment analyzer (Agilent). Library preparation was carried out using 0.5 μg of RNA with NEBNext Ultra II RNA library Prep Kit (New England BioLabs, E7770S) as per manufacturer’s instructions. Libraries were amplified for 4 total PCR cycles and purified with AMPure XP beads (Beckman Coulter Life Sciences, A63881). DNA concentration was measured with a Qubit Fluorometer (Thermo Fisher Scientific, Q32854) and fragment size was determined with a Fragment analyzer (Agilent).

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

characteristic: strain


text: Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: normal

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: 129 EpiSC

characteristic: cell line

text: epiblast cell

characteristic: cell type

text: house mouse

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: Protocols: EpiSCs were seeded at ~6 x 10^3 cells/cm2 in 6-well cell-culture plates (Corning, 3506) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015), and differentiated to pre-somitic mesoderm (PSM) as described below. Wild-type EpiSC lines were derived from E6.5 mouse embryos (129 background) and maintained in 6-well cell-culture plates (Corning, 3506) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015) in N2B27 medium (prepared as described in Ying Q.L. & Smith A.G., Methods Enzymol 365, 327-341 (2003)), supplemented with 20 ng/ml Activin A, 10 ng/ml bFGF and 1 µM XAV939 (EpiSC medium). Pbx1/Pbx2 double-knockout (Pbx1/2-DKO) EpiSC lines and lines carrying base-pair substitutions on the Msgn1 promoter that abolish PBX recruitment (pMsgn1-mut) were generated by CRISPR/Cas9. All differentiation was conducted in serum-free, feeder-free and monolayer conditions in chemically-defined N2B27-based media. Culture conditions followed a modified protocol from Loh K.M. et al., Cell 166, 451-467 (2016). EpiSCs were seeded at ~6 x 10^3 cells/cm2 in 6-well cell-culture plates pre-coated with 15 µg/ml human Fibronectin, and cultured in EpiSC medium for 24 h. EpiSCs were first differentiated towards anterior primitive streak (APS) using 30 ng/ml Activin A, 4 µM CHIR-99021, 20 ng/ml bFGF and 100 nM PIK-90 (APS medium) for 12 h, and then to pre-somitic mesoderm (PSM) using 1 µM A-83-01, 3 µM CHIR-99021, 250 nM LDN193189 and 20 ng/ml bFGF (PSM medium) for 36 h. At desired time-points (EpiSCs, 6 hours, 12 hours, 24 hours, 36 hours, 48 hours of differentiation), 50,000 cells per replicate were transposed as described in Buenrostro J.D.et al., Nat Methods 10, 1213-1218 (2013), with modifications based on Corces M.R. et al., Nat Methods 14, 959-962 (2017). Libraries were generated using the Ad1_noMX and Ad2.1-2.16 barcoded primers described in Buenrostro J.D.et al., Nat Methods 10, 1213-1218 (2013), and amplified for 10 total PCR cycles. Libraries were purified with AMPure XP beads (Beckman Coulter Life Sciences, A63881) to remove contaminating primer dimers and fragments larger than 1,000 bp. Library quality was assessed using the Fragment analyzer (Agilent) and quantitated by Qubit assay (Thermo Fisher Scientific, Q32854).

text: embryo

text: normal

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: embryo

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: 129 EpiSC

characteristic: cell line

text: epiblast cell

characteristic: cell type

text: house mouse

text: Protocols: EpiSCs were seeded at ~2 x 10^3 cells/cm2 in EpiSC medium in 150 mm tissue-culture dishes (Corning, 430599) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015), and differentiated to pre-somitic mesoderm (PSM) as described below. At desired time-points, cells were washed with PBS and fixed directly on the plate by the addition of 20 ml of PBS containing 1% (v/v) Formaldehyde Fixative Ultrapure EM Grade (Rockland, KHF001) for 8-10 min at RT. Fixation was stopped by 5 min incubation with 125 mM glycine at RT. After 2 washes with cold PBS, fixed cells were harvested in lysis buffer (50 mM Tris-HCl pH 8.1, 100 mM NaCl, 5 mM EDTA, 0.2% (w/v) NaN3, 0.5% (w/v) SDS, supplemented with 1x Protease inhibitor cocktail (Roche, 11836170001) and 1 mM PMSF) by extensive scraping using a pipette tip. Following centrifugation at 1,000 x g for 5 min at 4°C, the pellet was resuspended in 1 ml of FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% sodium deoxycholate, 150 mM NaCl, supplemented with 1x Protease inhibitor cocktail) per 0.5 ml of pellet. EpiSC lines were derived from E6.5 mouse embryos (129 background) and maintained in 6-well cell-culture plates (Corning, 3506) pre-coated with 15 µg/ml human Fibronectin (Merck Millipore, FC01015) in N2B27 medium (prepared as described in Ying Q.L. & Smith A.G., Methods Enzymol 365, 327-341 (2003)), supplemented with 20 ng/ml Activin A, 10 ng/ml bFGF and 1 µM XAV939 (EpiSC medium). All differentiation was conducted in serum-free, feeder-free and monolayer conditions in chemically-defined N2B27-based media. Culture conditions followed a modified protocol from Loh K.M. et al., Cell 166, 451-467 (2016). EpiSCs were seeded at ~6 x 10^3 cells/cm2 in 6-well cell-culture plates pre-coated with 15 µg/ml human Fibronectin, and cultured in EpiSC medium for 24 h. EpiSCs were first differentiated towards anterior primitive streak (APS) using 30 ng/ml Activin A, 4 µM CHIR-99021, 20 ng/ml bFGF and 100 nM PIK-90 (APS medium) for 12 h, and then to pre-somitic mesoderm (PSM) using 1 µM A-83-01, 3 µM CHIR-99021, 250 nM LDN193189 and 20 ng/ml bFGF (PSM medium) for 36 h. Chromatin was disrupted by sonication using a chilled Bioruptor Plus sonicator UCD-300 (Diagenode) for 8-12 pulses (cycles of 30 s on / 45 s off, high setting at 4°C) to obtain fragments of 200-500 bp in size. The extract was spun for 30 min at 20,000 x g at 4°C, and the soluble fraction was aliquoted and stored at -80°C until use. ChIP was performed with a protocol modified from that of Kolasinska-Zwierz P. et al., Nat Genet 41, 376-381 (2009). For each IP, 20 μg of chromatin were incubated overnight in 1 ml of FA buffer containing 1% N-lauroylsarkosine sodium salt with the following antibodies: PBX1 (polyclonal, Rabbit; Cell Signaling Technology, cat. nr. 4342, 1:100); PBX2 (polyclonal, Rabbit; Thermo Fisher Scientific, cat. nr. PA5-63964; 1:125); LEF1 (monoclonal, Rabbit; Cell Signaling Technology, cat. nr. 2230; 1:100). 10% of the IP volume of the chromatin was retained as an input reference. After overnight rotation at 4°C, 30 μl of washed and blocked magnetic Protein A (Thermo Fisher Scientific, 10006D) or Protein G Dynabeads (Thermo Fisher Scientific, 10003D) were added and the incubation continued for 2 additional h. Beads were extensively washed at RT as described in Kolasinska-Zwierz P. et al. DNA was eluted twice with 57 μl of elution buffer (1% (w/v) SDS in TE pH 8.0 with 250 mM NaCl) at 65°C, 15 min each time. Eluted DNA was incubated with 20 μg of RNase A for 30 min at 37°C and then with 20 μg of Proteinase K for 1 h at 55°C. Input DNA was also diluted in 114 ul of elution buffer and treated as ChIP samples. Crosslinks were reversed at 65°C overnight and DNA was purified using the QIAGEN MinElute PCR Purification Kit (QIAGEN, 28006) according to the manufacturer’s instructions. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645S) as per manufacturer’s instructions. Libraries were amplified for 5-7 total PCR cycles and purified with AMPure XP beads (Beckman Coulter Life Sciences, A63881). DNA concentration was measured with a Qubit Fluorometer (Thermo Fisher Scientific, Q32854) and fragment size was determined with a Fragment analyzer (Agilent).

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: embryo

text: normal

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9775:ChIPseq_LEF1_12h_Pbx1/2-DKO_Input

characteristic: Submitter Id

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9775:ChIPseq_LEF1_24h_Pbx1/2-DKO_Input

text: ArrayExpress

characteristic: broker name

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-9775:ChIPseq_LEF1_48h_Pbx1/2-DKO_Input

text: ArrayExpress

text: 129 EpiSC

characteristic: cell line

text: epiblast cell

characteristic: cell type

text: house mouse

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9775:ChIPseq_LEF1_EpiSC_Pbx1/2-DKO

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9775:ChIPseq_LEF1_EpiSC_Pbx1/2-DKO_Input

characteristic: Submitter Id

text: ArrayExpress

text: 129 EpiSC

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: normal

text: Pbx1 -/-; Pbx2 -/-

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WAGENINGEN UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:WAGENINGEN UNIVERSITY

text: Dandelion

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Taraxacum officinale

text: Taraxacum officinale

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WAGENINGEN UNIVERSITY

text: public

characteristic: SRA accession

text: Dandelion

text: France

text: Taraxacum officinale

text: Taraxacum officinale


text: WAGENINGEN UNIVERSITY

text: public

text: Dandelion

text: Netherlands

text: Taraxacum officinale

text: Test

text: Taraxacum officinale


text: Quadram Institute Bioscience

text: public

text: Tumour

characteristic: bio material

text: Quadram Institute Bioscience

characteristic: broker name

characteristic: cell line

text: PyMT-BO1 Whole Tumour

characteristic: cell type

text: Alastair McKee

characteristic: collected by

characteristic: collection date

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from Water Treated mouse 04

text: United Kingdom

text: Norwich

characteristic: geographic location (region and locality)

text: single-cell RNAseq

characteristic: investigation type

text: Mus musculus

text: Mus musculus

text: 10x Genomics

characteristic: sequencing method

text: F

characteristic: sex

text: PyMT-BO1 tumour

characteristic: tissue type

text: Water

characteristic: treatment

text: Breast Cancer


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: Tumour

text: Quadram Institute Bioscience

text: PyMT-BO1 Whole Tumour

text: Alastair McKee

text: house mouse

characteristic: common name

text: C57 BL/6 PyMT-BO1 Tumour from Water Treated mouse 05

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Norwich

text: single-cell RNAseq

text: Mus musculus

characteristic: organism

text: Mus musculus

text: 10x Genomics

text: F

text: PyMT-BO1 tumour

text: Water

text: Breast Cancer


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

text: public

characteristic: SRA accession

text: Tumour

characteristic: bio material

text: Quadram Institute Bioscience

characteristic: broker name

characteristic: cell line

text: PyMT-BO1 Whole Tumour

characteristic: cell type

text: Alastair McKee

characteristic: collected by

characteristic: collection date

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from ABX Treated mouse 01

text: United Kingdom

text: Norwich

characteristic: geographic location (region and locality)

text: single-cell RNAseq

text: Mus musculus

text: Mus musculus

text: 10x Genomics

text: F

text: PyMT-BO1 tumour

characteristic: title

text: VNMAA

text: Breast Cancer


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Tumour

text: Quadram Institute Bioscience

text: PyMT-BO1 Whole Tumour

text: Alastair McKee

text: house mouse

text: C57 BL/6 PyMT-BO1 Tumour from ABX Treated mouse 02

text: United Kingdom

text: Norwich

text: single-cell RNAseq

characteristic: investigation type

text: Mus musculus

text: Mus musculus

text: 10x Genomics

characteristic: sequencing method

text: F

characteristic: sex

text: PyMT-BO1 tumour

characteristic: tissue type

text: VNMAA

characteristic: treatment

text: Breast Cancer


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-META

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: human-gut

characteristic: human gut environmental package

text: human gut metagenome

characteristic: organism

text: human gut metagenome

text: human gut metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

tag: Namespace:CONSIGLIO PER LA RICERCA IN AGRICOLTURA ED ANALISI ECONOMIA AGRARIA (CREA)

characteristic: collection date

text: No-Tillage soil with crop rotation rep1

characteristic: description

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CONSIGLIO PER LA RICERCA IN AGRICOLTURA ED ANALISI ECONOMIA AGRARIA (CREA)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No-Tillage soil with crop rotation rep2

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: collection date

text: No-Tillage soil with crop rotation rep3

characteristic: description

text: soil

text: agricultural soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

text: host-associated

text: metagenome

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment


characteristic: ENA-LAST-UPDATE

text: public

text: No-Tillage soil with crop rotation rep4

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No-Tillage soil with crop rotation rep5

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: collection date

text: No-Tillage soil without crop rotation rep1

characteristic: description

text: soil

text: agricultural soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

text: host-associated

text: metagenome

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment


characteristic: ENA-LAST-UPDATE

text: public

text: No-Tillage soil without crop rotation rep2

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No-Tillage soil without crop rotation rep3

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: collection date

text: No-Tillage soil without crop rotation rep4

characteristic: description

text: soil

text: agricultural soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

text: host-associated

text: metagenome

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment


characteristic: ENA-LAST-UPDATE

text: public

text: Soil Tillage without crop rotation rep1

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Soil Tillage without crop rotation rep2

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: collection date

text: Soil Tillage without crop rotation rep3

characteristic: description

text: soil

text: agricultural soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

text: host-associated

text: metagenome

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment


characteristic: ENA-LAST-UPDATE

text: public

text: Soil Tillage without crop rotation rep4

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Soil Tillage with crop rotation rep1

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: collection date

text: Soil Tillage with crop rotation rep2

characteristic: description

text: soil

text: agricultural soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Italy

text: host-associated

text: metagenome

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment


characteristic: ENA-LAST-UPDATE

text: public

text: Soil Tillage with crop rotation rep3

text: soil

characteristic: environment (biome)

text: agricultural soil

text: soil

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: soil metagenome

characteristic: project name

text: soil metagenome

text: Illumina MiSeq

text: Long-term field experiment

characteristic: title


characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Soil Tillage with crop rotation rep4

text: soil

text: agricultural soil

text: soil

text: Italy

text: host-associated

text: metagenome

characteristic: investigation type

text: soil metagenome

text: soil metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Long-term field experiment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRA (INRA Toulouse)

characteristic: INSDC center name

text: public

tag: Namespace:INRA (INRA Toulouse)

text: tomato

characteristic: common name

text: Solanum lycopersicum

characteristic: organism

text: Solanum lycopersicum


characteristic: ENA-LAST-UPDATE

text: IMPERIAL COLLEGE LONDON

text: public

tag: Namespace:IMPERIAL COLLEGE LONDON

characteristic: collection date

text: Preterm

characteristic: description

text: Microbial community

characteristic: environment (biome)

text: Vaginal fornix

characteristic: environment (feature)

text: Vaginal fluid

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-vaginal

characteristic: human vaginal environmental package

text: metagenome

text: human vaginal metagenome

text: Vaginal microbiome in a pregnancy cohort

text: human vaginal metagenome

text: Illumina MiSeq

characteristic: target gene


characteristic: External Id

text: IMPERIAL COLLEGE LONDON

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Term

text: Microbial community

text: Vaginal fornix

text: Vaginal fluid

text: United Kingdom

text: human-vaginal

text: metagenome

characteristic: investigation type

text: human vaginal metagenome

characteristic: project name

text: human vaginal metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

tag: Namespace:Friedrich Miescher Institute for Biomedical Research

text: ArrayExpress

characteristic: broker name

text: B1A4 GFP-Puro

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Genomic DNA collected Cells grown in N2B27, 2iLIF medium Column purified 5µg genomic DNA was digested with MseI, PCR purified and ligated. Products were separately digested with NheI and PvuII, and purified. Sequencing libraries were generated using 35 amplification cycles and primers aatgatacggcgaccaccgagatctacacGCCAGTCCTCCGATTGA and caagcagaagacggcatacgagatBBBBBBAGTTCCTATTCCGAAGTTCCTATTCTCTA (B=barcode).

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: B1A4 GFP-Puro

characteristic: genotype

text: N2B27, 2i

characteristic: growth condition

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus


characteristic: External Id

text: Friedrich Miescher Institute for Biomedical Research

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: B1A4 GFP-Puro

text: embryonic stem cell

text: house mouse

text: embryo

text: normal

text: B1A4 GFP-Puro

text: N2B27, 2i

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: B1A4 GFP-Puro

text: embryonic stem cell

text: house mouse

text: Protocols: Genomic DNA collected Cells grown in N2B27, 3μΜ Chiron medium Column purified 5µg genomic DNA was digested with MseI, PCR purified and ligated. Products were separately digested with NheI and PvuII, and purified. Sequencing libraries were generated using 35 amplification cycles and primers aatgatacggcgaccaccgagatctacacGCCAGTCCTCCGATTGA and caagcagaagacggcatacgagatBBBBBBAGTTCCTATTCCGAAGTTCCTATTCTCTA (B=barcode).

text: embryo

text: normal

text: B1A4 GFP-Puro

text: N2B27, 3M Chir

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


text: DTU-GE

text: public

text: DTU-GE

text: National Microbiology Reference Laboratory

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

text: Zimbabwe:Africa:Harare

characteristic: geo loc name

text: Zimbabwe

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: Human

characteristic: isolation source host associated

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella Typhi

characteristic: serovar

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: No

text: Zimbabwe:Africa:Gweru

text: Zimbabwe

text: not provided

text: Homo sapiens

text: Yes

text: Human

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Salmonella Typhi


text: The University of Manchester

text: public

tag: Namespace:The University of Manchester

text: 8 to 10

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: innate lymphoid cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: FACS sorted lymphocytes were isolated from small intestine of transgenic mice. Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf

characteristic: description

text: adult

characteristic: developmental stage

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: cell line

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates transfected with gRNA sequence AGAAGCACGGGTAGACCCTC, Ubn2 KO RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

characteristic: disease

text: Ubn2 -/-

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 48h transfected with gRNA sequence AGAAGCACGGGTAGACCCTC, Ubn2 KO RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

text: Ubn2 -/-

text: SerumLIF

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


text: public

text: ArrayExpress

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates transfected with gRNA AGTCCGGGATGTCATCCCCA, Ubn2 KO RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

text: Ubn2 -/-

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 48h transfected with gRNA AGTCCGGGATGTCATCCCCA, Ubn2 KO RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

text: Ubn2 -/-

text: SerumLIF

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: cell line

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates TbC1 parental cell line RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

characteristic: disease

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 48h TbC1 parental cell line RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

text: wild type genotype

text: SerumLIF

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates transfected with gRNAs CTGGAAAGCACCTTGAGGG and GCACACGTGGCGTGCGGCC, WT clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 48h transfected with gRNAs CTGGAAAGCACCTTGAGGG and GCACACGTGGCGTGCGGCC, WT clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

text: wild type genotype

text: SerumLIF

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: mouse embryonic stem cell

text: house mouse

text: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates transfected with gRNA AGTCCGGGATGTCATCCCCA, WT clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)

text: embryo

text: normal

characteristic: disease

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus