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E-MTAB-10154:W12 Clone A5

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characteristic: cell line

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text: human

characteristic: common name

text: Protocols: W12 cells were cultured in a monolayer to restrict cell differentiation and maintain the phenotype of the basal epithelial. Cells were collected at the lowest possible passage after cloning (p3 to p8) to minimise any effects of genomic instability caused byderegulated HPV16 oncogenes. Biotinylated RNA bait library generation for HISC Four consecutive and non-overlapping fragments from the pSP64 HPV16 bacterial artificial chromosome were PCR amplified using the Expand High Fidelity PCR system (Roche). This resulted in complete coverage of the HPV16 genome. T7 promoter sequences (Roche) were added to one side of the PCR product during this PCR amplification, enabling subsequent directional in vitro transcription. Sequences were in vitro transcribed in the presence of biotinUTP and purified as described above, followed by fragmentation to 120 nt with 4 nM magnesium chloride at 95°C for 7 min. Fragmented biotinylated RNA was purified by Isopropanol precipitation followed by two subsequent washes with 75% ethanol. Cells were lysed on ice for 30 minutes in 50 ml freshly prepared icecold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet (Roche complete, EDTA-free)). Nuclei were pelleted (650 g for 5 minutes at 4˚C), washed once with 1.25 x NEBuffer 2, and then resuspended in 1.25 x NEBuffer 2 to make aliquots of 5-6 million cells. SDS was added (0.3% final concentration) and the nuclei were incubated at 37˚C for one hour (950 rpm). Triton X-100 was added to a final concentration of 1.7 % and the nuclei were incubated at 37˚C for one hour (950 rpm). Ligation of the custom sequence adapters was done in solution by adding 4 microlitre adaptors (30 micromolar) and 1600 units T4 DNA ligase (NEB). The ligation was carried at for 2 hours at room temperature on a rotating wheel in 1x ligation buffer (NEB). Pre-capture PCR amplification was carried out using the custom primers PE PCR 1.0.33 and PE PCR 2.0.33 with 7-8 cycles. The amplified libraries were purified with AMPure XP beads (Beckman Coulter) and the quantity and the quality was assessed by Bioanalyzer profiles (Agilent Technologies). 500 ng of genomic library DNA were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 microlitre dH2O. 2.5 microgram mouse cot-1 DNA (Invitrogen) and 2.5 microgram sheared salmon sperm DNA (Ambion) were added as blocking agents. To prevent concatemer formation during hybridization, 1.5 microlitre blocking mix (300 mircomoles) was added (equimolar mix of P5_b1_for_33, P5_b1_rev_33, P7_b2_for and P7B2_rev). Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). The DNA was denatured at 95˚C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65˚C, in hybridization buffer (5 x SSPE (Gibco), 5 x Denhardt's solution (Invitrogen), 5 mM EDTA (Gibco), 0.1 % SDS (Promega)) for 24 hours, in a total reaction volume of 30 microlitre. Captured DNA/RNA hybrids were enriched using DynabeadsMyOne Streptavidin T1 beads (Life Technologies) in binding buffer (1 M NaCl, 10 mM TrisHCl pH 7.5, 1 mM EDTA) for 30 minutes at room temperature. After washing (once in washbuffer 1 (1 x SSC, 0.1 % SDS) for 15 minutes at room temperature, followed by three washes in wash buffer 2 (0.1 x SSC, 0.1 % SDS) for 10 minutes each at 65˚C), the streptavidin beads(with bound captured DNA/RNA) were re-suspended in 30 microlitre 1 x NEBuffer 2. Post-capture PCR amplification was carried out using between six to nine cycles using primer pairs that consisted of one TruSeq adapter reverse compliment and the TruSeq universal adapter from streptavidin beads in multiple parallel reactions, which were then pooled to purify the PCR products using AMPure XP beads (Beckman Coulter).

characteristic: description

text: squamous cell neoplasm

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

characteristic: strain

text: W12 Clone A5

characteristic: title


E-MTAB-10154:W12 Clone D2

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10154:W12 Clone D2

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone D2


E-MTAB-10154:W12 Clone F

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: SRA accession

text: E-MTAB-10154:W12 Clone F

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: F

text: W12 Clone F


E-MTAB-10154:W12 Clone G2

text: EMBL-EBI

text: public

text: E-MTAB-10154:W12 Clone G2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone G2


E-MTAB-10154:W12 Clone H

text: EMBL-EBI

text: public

text: E-MTAB-10154:W12 Clone H

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: H

text: W12 Clone H


E-MTAB-10152:Ncx Hi-C rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10152:Ncx Hi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: W12 cells were cultured in a monolayer to restrict cell differentiation and maintain the phenotype of the basal epithelial. Cells were collected at the lowest possible passage after cloning (p3 to p8) to minimise any effects of genomic instability caused by deregulated HPV16 oncogenes. Cells were lysed on ice for 30 minutes in 50 ml freshly prepared icecold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet (Roche complete, EDTA-free)). Nuclei were pelleted (650 g for 5 minutes at 4˚C), washed once with 1.25 x NEBuffer 2, and then resuspended in 1.25 x NEBuffer 2 to make aliquots of 5-6 million cells for digestion. SDS was added (0.3% final concentration) and the nuclei were incubated at 37˚C for one hour (950 rpm). Triton X-100 was added to a final concentration of 1.7 % and the nuclei were incubated at 37˚C for one hour (950 rpm). Restriction digest was performed overnight at 37˚C (950 rpm) using 800 units MboI (NEB) per 5 million cells. Restriction fragment ends were filled in using Klenow (NEB) with dCTP, dGTP, dTTP and biotin-14-dATP, and the blunt-ended DNA was ligated following an in-nucleus ligation protocol. Excess salts and enzymes were removed by centrifugation (600 g for 5 minutes at 4˚C) and the cell pellet was re-suspended in 995 µl of 1x ligation buffer (NEB) supplemented with BSA (100 µg/mL final concentration). Ligation was carried out using 2000 units of T4 DNA ligase (NEB) per 5 Mio starting material of cells, at 16˚C for 4 hours, followed by 30 min at room temperature. Chromatin was then decrosslinked overnight at 65˚C in the presence of proteinase K (Roche), purified by phenol and phenol-chloroform extractions, precipitated with ethanol and sodium acetate and re-suspended in TLE (10 mM Tris-HCl pH 8.0; 0.1 mM EDTA). 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20˚C to remove of biotin from nonligated fragment ends, followed by phenol/chloroform purification and DNA precipitation overnight at -20˚C. DNA was sheared to an average size of 400 bp using the Covaris E220 (settings: duty factor: 10%; peak incident power: 140W; cycles per burst: 200; time: 55 seconds). End-repairing of the sheared DNA (using T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB)) was followed by dATP addition (Klenow exo-, NEB) and a double-sided size selection using AMPure XP beads (Beckman Coulter) to isolate DNA ranging from 250 to 550 bp. Biotin-marked ligation junctions were immobilised using MyOne Streptavidin C1 Dynabeads (Invitrogen) in binding buffer (5 mM Tris-HCl pH 8.0, 0.5 mM EDTA, 1M NaCl) and after stringent washing in the same buffer at 55°C for 10 min ligated to the custom SCRiBL adapter using 1600 units of T4 DNA ligase (NEB) for 2 hours at room temperature. These adapters were generated by annealing SCRiBL_adapter_1 and SCRiBL_adapter_2 (table X). The immobilised Hi-C libraries were amplified using the custom primers PE PCR 1.0.33 and PE PCR 2.0.33 with 7-9 cycles. After PCR amplification, the HiC libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).

text: normal

text: Hi-C

characteristic: experiment

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: Normal Cervix

characteristic: strain

text: Ncx Hi-C rep I

characteristic: title


E-MTAB-10152:Ncx Hi-C rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10152:Ncx Hi-C rep II

text: ArrayExpress

text: epithelial cell

text: human

text: normal

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: Normal Cervix

text: Ncx Hi-C rep II


E-MTAB-10152:W12 Clone D2 Hi-C rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: SRA accession

text: E-MTAB-10152:W12 Clone D2 Hi-C rep I

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone D2

text: W12 Clone D2 Hi-C rep I


E-MTAB-10152:W12 Clone D2 Hi-C rep II

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone D2 Hi-C rep II

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

characteristic: disease

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone D2

text: W12 Clone D2 Hi-C rep II


E-MTAB-10152:W12 Clone G2 Hi-C rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone G2 Hi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Hi-C

characteristic: experiment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: W12 Clone G2

characteristic: strain

text: W12 Clone G2 Hi-C rep I


E-MTAB-10152:W12 Clone G2 Hi-C rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10152:W12 Clone G2 Hi-C rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone G2

text: W12 Clone G2 Hi-C rep II

characteristic: title


E-MTAB-10152:W12 Clone H Hi-C rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10152:W12 Clone H Hi-C rep I

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone H

text: W12 Clone H Hi-C rep I


E-MTAB-10152:W12 Clone H Hi-C rep II

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone H Hi-C rep II

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

text: Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone H

text: W12 Clone H Hi-C rep II


E-MTAB-10152:W12 Clone A5 cHi-C rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone A5 cHi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: W12 cells were cultured in a monolayer to restrict cell differentiation and maintain the phenotype of the basal epithelial. Cells were collected at the lowest possible passage after cloning (p3 to p8) to minimise any effects of genomic instability caused by deregulated HPV16 oncogenes. Capture RNA bait library design 120-mer capture RNA baits were bioinformatically designed to both ends of MboI restriction fragments overlapping the HPV16 genome. Requirements for target sequences were as follows: GC content between 25% and 65%, no more than two consecutive Ns within the target sequences, and maximum distance to a MboI restriction site 330 bp. For short MboI fragments, where 120-mer RNA baits originating from both ends would have overlapped (potentially interfering with optimal hybridization to Hi-C libraries), only the Watson (coding or sense) strand was used for capture RNA bait design, and if necessary the baits were trimmed to minimum length no shorter than 97 nt. This yielded 16 RNA bait sequences covering the MboI restriction fragment ends of the entire HPV16 genome, with the exception of two fragments too short (18 and 63 bp, respectively) for capture RNA bait design. Cells were lysed on ice for 30 minutes in 50 ml freshly prepared icecold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet (Roche complete, EDTA-free)). Nuclei were pelleted (650 g for 5 minutes at 4˚C), washed once with 1.25 x NEBuffer 2, and then resuspended in 1.25 x NEBuffer 2 to make aliquots of 5-6 million cells for digestion. SDS was added (0.3% final concentration) and the nuclei were incubated at 37˚C for one hour (950 rpm). Triton X-100 was added to a final concentration of 1.7 % and the nuclei were incubated at 37˚C for one hour (950 rpm). Restriction digest was performed overnight at 37˚C (950 rpm) using 800 units MboI (NEB) per 5 million cells. Restriction fragment ends were filled in using Klenow (NEB) with dCTP, dGTP, dTTP and biotin-14-dATP, and the blunt-ended DNA was ligated following an in-nucleus ligation protocol. Excess salts and enzymes were removed by centrifugation (600 g for 5 minutes at 4˚C) and the cell pellet was re-suspended in 995 µl of 1x ligation buffer (NEB) supplemented with BSA (100 µg/mL final concentration). Ligation was carried out using 2000 units of T4 DNA ligase (NEB) per 5 Mio starting material of cells, at 16˚C for 4 hours, followed by 30 min at room temperature. Chromatin was then decrosslinked overnight at 65˚C in the presence of proteinase K (Roche), purified by phenol and phenol-chloroform extractions, precipitated with ethanol and sodium acetate and re-suspended in TLE (10 mM Tris-HCl pH 8.0; 0.1 mM EDTA). 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20˚C to remove of biotin from nonligated fragment ends, followed by phenol/chloroform purification and DNA precipitation overnight at -20˚C. DNA was sheared to an average size of 400 bp using the Covaris E220 (settings: duty factor: 10%; peak incident power: 140W; cycles per burst: 200; time: 55 seconds). End-repairing of the sheared DNA (using T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB)) was followed by dATP addition (Klenow exo-, NEB) and a double-sided size selection using AMPure XP beads (Beckman Coulter) to isolate DNA ranging from 250 to 550 bp. Biotin-marked ligation junctions were immobilised using MyOne Streptavidin C1 Dynabeads (Invitrogen) in binding buffer (5 mM Tris-HCl pH 8.0, 0.5 mM EDTA, 1M NaCl) and after stringent washing in the same buffer at 55°C for 10 min ligated to the custom SCRiBL adapter using 1600 units of T4 DNA ligase (NEB) for 2 hours at room temperature. These adapters were generated by annealing SCRiBL_adapter_1 and SCRiBL_adapter_2 (table X). The immobilised Hi-C libraries were amplified using the custom primers PE PCR 1.0.33 and PE PCR 2.0.33 with 7-9 cycles. After PCR amplification, the HiC libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies). Solution hybridization Region Capture of Hi-C libraries. 500 ng of Hi-C library DNA were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 microlitre dH2O. 2.5 microgram mouse cot-1 DNA (Invitrogen) and 2.5 microgram sheared salmon sperm DNA (Ambion) were added as blocking agents. To prevent concatemer formation during hybridization, 1.5 microlitre blocking mix (300 mircomoles) was added (equimolar mix of P5_b1_for_33, P5_b1_rev_33, P7_b2_for and P7B2_rev). Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). The DNA was denatured at 95˚C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65˚C, in hybridization buffer (5 x SSPE (Gibco), 5 x Denhardt's solution (Invitrogen), 5 mM EDTA (Gibco), 0.1 % SDS (Promega)) for 24 hours, in a total reaction volume of 30 microlitre. Captured DNA/RNA hybrids were enriched using DynabeadsMyOne Streptavidin T1 beads (Life Technologies) in binding buffer (1 M NaCl, 10 mM TrisHCl pH 7.5, 1 mM EDTA) for 30 minutes at room temperature. After washing (once in washbuffer 1 (1 x SSC, 0.1 % SDS) for 15 minutes at room temperature, followed by three washes in wash buffer 2 (0.1 x SSC, 0.1 % SDS) for 10 minutes each at 65˚C), the streptavidin beads(with bound captured DNA/RNA) were re-suspended in 30 microlitre 1 x NEBuffer 2. Post-capture PCR amplification was carried out using between six to nine cycles using primer pairs that consisted of one TruSeq adapter reverse compliment and the TruSeq universal adapter from streptavidin beads in multiple parallel reactions, which were then pooled to purify the PCR products using AMPure XP beads (Beckman Coulter).

text: squamous cell neoplasm

characteristic: disease

text: HPV capture Hi-C

characteristic: experiment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: W12 Clone A5

text: W12 Clone A5 cHi-C rep I


E-MTAB-10152:W12 Clone A5 cHi-C rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10152:W12 Clone A5 cHi-C rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone A5

characteristic: strain

text: W12 Clone A5 cHi-C rep II


E-MTAB-10152:W12 Clone D2 cHi-C rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10152:W12 Clone D2 cHi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone D2

text: W12 Clone D2 cHi-C rep I

characteristic: title


E-MTAB-10152:W12 Clone D2 cHi-C rep II

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone D2 cHi-C rep II

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone D2

text: W12 Clone D2 cHi-C rep II


E-MTAB-10152:W12 Clone F cHi-C rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone F cHi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: squamous cell neoplasm

characteristic: disease

text: HPV capture Hi-C

characteristic: experiment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone F

text: W12 Clone F cHi-C rep I


E-MTAB-10152:W12 Clone F cHi-C rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10152:W12 Clone F cHi-C rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: W12 Clone F

text: W12 Clone F cHi-C rep II


E-MTAB-10152:W12 Clone G2 cHi-C rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10152:W12 Clone G2 cHi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone G2

characteristic: strain

text: W12 Clone G2 cHi-C rep I


E-MTAB-10152:W12 Clone G2 cHi-C rep II

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone G2 cHi-C rep II

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone G2

text: W12 Clone G2 cHi-C rep II

characteristic: title


E-MTAB-10152:W12 Clone H cHi-C rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10152:W12 Clone H cHi-C rep I

text: ArrayExpress

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

characteristic: disease

text: HPV capture Hi-C

characteristic: experiment

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone H

text: W12 Clone H cHi-C rep I


E-MTAB-10152:W12 Clone H cHi-C rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10152:W12 Clone H cHi-C rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: HPV capture Hi-C

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 Clone H

text: W12 Clone H cHi-C rep II


E-MTAB-10155:NCx6p4 rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10155:NCx6p4 rep I

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: W12 cells were cultured in a monolayer to restrict cell differentiation and maintain the phenotype of the basal epithelial. Cells were collected at the lowest possible passage after cloning (p3 to p8) to minimise any effects of genomic instability caused byderegulated HPV16 oncogenes. Cells were lysed on ice for 30 minutes in 50 ml freshly prepared icecold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet (Roche complete, EDTA-free)). Total RNA was isolated and subjected to Ribo-Zero rRNA depletion and DNAse treatment. cDNA libraries were prepared from the ribo-depleted RNA with the TruSeq™ RNA and DNA Sample Prep Kit (Illumina)

text: normal

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: Normal cervix

text: NCx6p4 rep I


E-MTAB-10155:NCx6p4 rep II

text: EMBL-EBI

text: public

text: E-MTAB-10155:NCx6p4 rep II

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: Normal cervix

characteristic: strain

text: NCx6p4 rep II


E-MTAB-10155:W12 clone A5 rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10155:W12 clone A5 rep I

text: ArrayExpress

characteristic: cell line

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone A5

text: W12 clone A5 rep I

characteristic: title


E-MTAB-10155:W12 clone A5 rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10155:W12 clone A5 rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone A5

text: W12 clone A5 rep II


E-MTAB-10155:W12 clone D2 rep I

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

characteristic: INSDC last update

text: public

text: E-MTAB-10155:W12 clone D2 rep I

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone D2

text: W12 clone D2 rep I


E-MTAB-10155:W12 clone D2 rep II

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10155:W12 clone D2 rep II

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: W12 clone D2

text: W12 clone D2 rep II


E-MTAB-10155:W12 clone F rep I

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10155:W12 clone F rep I

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone F

characteristic: strain

text: W12 clone F rep I


E-MTAB-10155:W12 clone F rep II

text: EMBL-EBI

text: public

text: E-MTAB-10155:W12 clone F rep II

text: ArrayExpress

characteristic: cell line

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone F

text: W12 clone F rep II

characteristic: title


E-MTAB-10155:W12 clone G2 rep I

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10155:W12 clone G2 rep I

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone G2

text: W12 clone G2 rep I


E-MTAB-10155:W12 clone G2 rep II

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMBL-EBI

characteristic: INSDC last update

text: public

text: E-MTAB-10155:W12 clone G2 rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone G2

text: W12 clone G2 rep II


E-MTAB-10155:W12 clone H rep I

text: EMBL-EBI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10155:W12 clone H rep I

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

characteristic: organism part

text: Homo sapiens

text: W12 clone H

text: W12 clone H rep I


E-MTAB-10155:W12 clone H rep II

characteristic: External Id

text: EMBL-EBI

text: public

text: E-MTAB-10155:W12 clone H rep II

text: ArrayExpress

characteristic: broker name

text: epithelial cell

characteristic: cell type

text: human

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 clone H

characteristic: strain

text: W12 clone H rep II


E-MTAB-10155:W12S2p31 rep I

text: EMBL-EBI

text: public

text: E-MTAB-10155:W12S2p31 rep I

text: ArrayExpress

characteristic: cell line

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: squamous cell neoplasm

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 parental

text: W12S2p31 rep I

characteristic: title


E-MTAB-10155:W12S2p31 rep II

characteristic: ENA-CHECKLIST

text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10155:W12S2p31 rep II

text: ArrayExpress

text: epithelial cell

text: human

text: squamous cell neoplasm

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: cervix epithelium

text: Homo sapiens

text: W12 parental

text: W12S2p31 rep II


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

tag: Namespace:Wallenberg Centre for Molecular Medicine (WCMM), Linkoping University, Linkoping, Sweden Department of Biomedical and Clinical Sciences, Division of Molecular Medicine and Virology, Faculty of Health Science, Linkoping University, Linkoping, Sweden

text: ArrayExpress

text: human

text: Protocols: 500 000 human melanoma cells (M010817) were harvested for CUT&RUN. Antibodies were incubated overnight at a 1:100 dilution. CUTANA pAG-MNase was used at 0.5X concentration and digestion was performed for 30 minutes. Phenol:Chloroform KAPA HyperPrep Kit with KAPA DUI Adapters

text: melanoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: FLAG


text: Wallenberg Centre for Molecular Medicine (WCMM), Linkoping University, Linkoping, Sweden Department of Biomedical and Clinical Sciences, Division of Molecular Medicine and Virology, Faculty of Health Science, Linkoping University, Linkoping, Sweden

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: melanoma

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: UMR CNRS 5557 Ecologie Microbienne

text: public

tag: Namespace:UMR CNRS 5557 Ecologie Microbienne

text: metagenome

text: metagenome


text: UMR CNRS 5557 Ecologie Microbienne

text: public

text: Mar_R1A_Rhizophora_Surface_RepA.fastq.gz

text: metagenome

text: metagenome


text: UMR CNRS 5557 Ecologie Microbienne

text: public

text: Mar_R1B_Rhizophora_Surface_RepB.fastq.gz

text: metagenome

text: metagenome


text: UMR CNRS 5557 Ecologie Microbienne

text: public

text: Mar_R1C_Rhizophora_Surface_RepC.fastq.gz

text: metagenome

text: metagenome


text: UMR CNRS 5557 Ecologie Microbienne

text: public

text: 170421_SN1126_A_L001_JEU-5_AdapterTrimmed_R1.fastq.gz

text: metagenome

text: metagenome


text: UMR CNRS 5557 Ecologie Microbienne

text: public

text: 170421_SN1126_A_L001_JEU-5_AdapterTrimmed_R2.fastq.gz

text: metagenome

text: metagenome


text: University of Iceland

text: public

tag: Namespace:University of Iceland

unit: L

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

text: Skalafell

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Skalafell shoulder near road

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

text: Shotgun metagenome sequences of Anthelia biocrust samples.

characteristic: project name

unit: g

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: cooled

characteristic: storage conditions (fresh/frozen/other)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Skalafell shoulder near road

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: cooled

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Anthelia biocrust

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Vesturoeraefi-Bruaroeraefi

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)

text: BruA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Skalafell oexl naerri vegi

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: cooled

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Anthelia biocrust

text: lowland rocky

text: rocky

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Innkeyrsla Thveras21

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: dry

characteristic: storage conditions (fresh/frozen/other)

text: OSA


Laki L1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Laki L1

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Laki L1

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Laki L1

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Laki L1SUB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Laki L1SUB

text: Anthelia biocrust

text: Laki L1SUB

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Laki L1

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Laki L2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Laki L2

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Laki L2

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Laki L1

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Laki L2a

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Laki L2a

text: Anthelia biocrust

text: Laki L2a

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Laki L1

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Laki L2aSUB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Laki L2aSUB

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Laki L2aSUB

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Laki L1

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Skaftartunga S1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Skaftartunga S1

text: Anthelia biocrust

text: Skaftartunga S1

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Skaftartunga S1

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Skaftartunga S2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Skaftartunga S2

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Skaftartunga S2

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Skaftartunga S2

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Skaftartunga S3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Skaftartunga S3

text: Anthelia biocrust

text: Skaftartunga S3

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Skaftartunga S3

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Skaftartunga S3SUB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Skaftartunga S3SUB

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Skaftartunga S3SUB

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Skaftartunga S3

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Laki L3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Laki L3

text: Anthelia biocrust

text: Laki L3

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Laki L3

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Fjallabak FB2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Fjallabak FB2

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Fjallabak FB2

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: geographic location (altitude)

text: Iceland

text: Fjallabak FB-2

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Fjallabak FB2a

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

text: public

text: Fjallabak FB2a

text: Anthelia biocrust

text: Fjallabak FB2a

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Fjallabak FB-2

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Fjallabak F1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Fjallabak F1

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Fjallabak F1

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Fjallabak F1

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Fjallabak F1a

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Fjallabak F1a

text: Anthelia biocrust

text: Fjallabak F1a

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Fjallabak F1

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Fjallabak F2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Fjallabak F2

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Fjallabak F2

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Fjallabak F2

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Fjallabak F3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Fjallabak F3

text: Anthelia biocrust

text: Fjallabak F3

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Fjallabak F3

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Gagnheidi G3

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Anthelia biocrust

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Gagnheidi G2

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

characteristic: description

text: alpine tundra

characteristic: environment (biome)

text: seasonal snow patch

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: eunis habitat

characteristic: geographic location (altitude)

text: Iceland

text: Gagnheidi G1

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

characteristic: title


Skjaldbreidur

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

text: public

text: Skjaldbreidur

text: Anthelia biocrust

text: Skjaldbreidur

text: alpine tundra

text: seasonal snow patch

text: soil

text: Iceland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Skjaldbreidur SK-1

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: Nextera XT

characteristic: library construction method

characteristic: library reads sequenced

characteristic: nucleic acid extraction

text: soil crust metagenome

characteristic: project name

characteristic: sample volume or weight for DNA extraction

characteristic: sample weight for DNA extraction

text: soil crust metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: frozen

characteristic: storage conditions (fresh/frozen/other)


Stjornarsandur

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Stjornarsandur

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: collection date

text: Anthelia biocrust

characteristic: common name

text: Stornarsandur

characteristic: description

text: lowland sandy

characteristic: environment (biome)

text: delta

characteristic: environment (feature)

text: soil

characteristic: environment (material)

characteristic: geographic location (altitude)

text: Iceland

text: Stjornarsandur naerri Breidbala

text: metagenome

text: Nextera XT

text: soil crust metagenome

characteristic: organism

text: soil crust metagenome

text: Illumina

text: soil

text: frozen

text: StjsB

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

text: public

text: wine grape

text: Vitis vinifera

text: Vitis vinifera

text: Vv cv Vinhao


name: E-MTAB-10167:Danio rerio AB wildtype_shield stage

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University

unit: hour

characteristic: age

text: ArrayExpress

characteristic: broker name

text: zebrafish

text: Protocols: Zebrafish embryos (obtained from wild-type strain AB) were collected at the shield stage and tail-bud stage, which correspond to 6 hpf and 10 hpf, respectively. Total RNA was extracted with miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer’s instructions. Quality of extracted RNA was assessed using the NanoDrop ND-1000 Spectrophotometer (ThermoFisher Scientific) and by agarose gel electrophoresis. Samples were stored at -80 ℃ Libraries for sequencing were prepared with TruSeq stranded mRNA library prep kit (Illumina) according to the manufacturer’s instructions.

text: gastrula shield

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

text: Danio rerio

text: AB

characteristic: strain

text: Danio rerio AB wildtype_shield stage


name: E-MTAB-10167:Danio rerio AB wildtype_tail bud stage

text: Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

characteristic: common name

characteristic: description

text: gastrula bud

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: whole organism

text: Danio rerio

text: AB

text: Danio rerio AB wildtype_tail bud stage


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Universidad Peruana Cayetano Heredia

text: public

tag: Namespace:Universidad Peruana Cayetano Heredia

characteristic: collection date

text: Untreated sediment

text: sediment

characteristic: environment (biome)

text: sediment

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

characteristic: organism

text: ena-STUDY-Universidad Peruana Cayetano Heredia-02-03-2021-00:09:24:612-1404

characteristic: project name

text: soil metagenome

text: sediment

characteristic: sediment environmental package

text: shotgun

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universidad Peruana Cayetano Heredia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Aerated sediment

characteristic: description

text: sediment

text: sediment

text: sediment

text: United Kingdom

text: metagenome

text: soil metagenome

text: soil metagenome

text: sediment

text: shotgun


characteristic: ENA-FIRST-PUBLIC

text: Universidad Peruana Cayetano Heredia

text: public

characteristic: collection date

text: addition of low molecular weight organic acids

text: sediment

characteristic: environment (biome)

text: sediment

characteristic: environment (feature)

text: sediment

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: soil metagenome

text: soil metagenome

text: sediment

text: shotgun


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universidad Peruana Cayetano Heredia

characteristic: INSDC center name

text: public

text: Addition of low molecular weight organic acids

text: sediment

text: sediment

text: sediment

text: United Kingdom

text: metagenome

characteristic: investigation type

text: soil metagenome

characteristic: organism

characteristic: project name

text: soil metagenome

text: sediment

characteristic: sediment environmental package

text: shotgun

characteristic: sequencing method

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Universidad Peruana Cayetano Heredia

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Addition of both aeration and low molecular weight organic acids

characteristic: description

text: sediment

characteristic: environment (biome)

text: sediment

characteristic: environment (feature)

text: sediment

text: United Kingdom

text: metagenome

text: soil metagenome

text: soil metagenome

text: sediment

text: shotgun


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE UNIVERSITY OF QUEENSLAND

characteristic: INSDC center name

text: public

tag: Namespace:THE UNIVERSITY OF QUEENSLAND

text: human

characteristic: common name

text: RNA derived from Homo Sapiens cortical organoids for 150bp paired-end library construction, for Illumina sequencing.

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina WT Sample 1


text: THE UNIVERSITY OF QUEENSLAND

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina WT Sample 2


text: THE UNIVERSITY OF QUEENSLAND

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina WT Sample 3

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: THE UNIVERSITY OF QUEENSLAND

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina WT Sample 4


text: THE UNIVERSITY OF QUEENSLAND

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina WT Sample 5


text: THE UNIVERSITY OF QUEENSLAND

characteristic: INSDC last update

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina AT Sample 6


text: THE UNIVERSITY OF QUEENSLAND

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina AT Sample 7


text: THE UNIVERSITY OF QUEENSLAND

text: public

characteristic: SRA accession

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina AT Sample 8


text: THE UNIVERSITY OF QUEENSLAND

text: public

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina AT Sample 9


text: THE UNIVERSITY OF QUEENSLAND

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens cortical organoids PE150 sequencing Illumina AT Sample 10


text: public

tag: Namespace:INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW

text: baker's yeast

text: S. cerevisiae Mex67-AA (with rapamycin), W303, 38°C 15min, Nab2 overexpression, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW

text: public

text: baker's yeast

text: S. cerevisiae Mex67-AA (no rapamycin), W303, 38°C 15min, Nab2 overexpression, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_NORAPA_1

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (no rapamycin), W303, 38°C 15min, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_NORAPA_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (no rapamycin), W303, 38°C 15min, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_NORAPA_pNAB2_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (no rapamycin), W303, 38°C 15min, Nab2 overexpression, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_NORAPA_pPAB1_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (no rapamycin), W303, 38°C 15min, Pab1 overexpression, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_1

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_pNAB2_1

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, Nab2 overexpression, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_pNAB2_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, Nab2 overexpression, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW

text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_pPAB1_1

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, Pab1 overexpression, biological replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: W303_MEX67-AA_pab1_delta_RRM4_38C_RAPA_pPAB1_2

text: baker's yeast

text: S. cerevisiae Mex67-AA, pab1 deltaRRM4 (with rapamycin), W303, 38°C 15min, Pab1 overexpression, biological replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

text: baker's yeast

text: S. cerevisiae Mex67-AA (no rapamycin), W303, 25°C 15min, Nab2 overexpression

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: WETSUS

text: public

characteristic: collection date

text: Soil

text: soil

text: soil

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

characteristic: investigation type

text: uncultured bacterium

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: Soil