text: ESALQ/USP

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characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: Plants in field conditions

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text: Twelve months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar F36-819

text: Replicate 2 of sugarcane culm in October:Lane 1


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

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text: public

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characteristic: common name

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar F36-819

characteristic: organism

text: Twelve months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819

text: Replicate 2 of sugarcane culm in October:Lane 2

characteristic: title


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Twelve months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar F36-819

text: Replicate 2 of sugarcane culm in October:Lane 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

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characteristic: SRA accession

characteristic: Submitter Id

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text: Plants in field conditions

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text: Six months

text: Soil

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text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in April:Lane 4


text: ESALQ/USP

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characteristic: collection date

characteristic: common name

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Six months

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in April:Lane 5


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

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text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar SP80-3280

characteristic: organism

text: Six months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in April:Lane 6

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: Plants in field conditions

text: Saccharum spontaneum

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characteristic: plant structure

text: Saccharum spontaneum


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characteristic: SRA accession

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text: Plants in field conditions

text: Saccharum spontaneum

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text: Saccharum spontaneum


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characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: genotype

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characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum spontaneum

text: Six months

text: Soil

text: Culm

text: Saccharum spontaneum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar F36-819

characteristic: organism

text: Twelve months

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text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819

text: Replicate 3 of sugarcane culm in October:Lane 4

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

characteristic: INSDC last update

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text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Twelve months

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characteristic: plant structure

text: Saccharum hybrid cultivar F36-819

text: Replicate 3 of sugarcane culm in October:Lane 5


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Twelve months

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819

text: Replicate 3 of sugarcane culm in October:Lane 6


characteristic: ENA-CHECKLIST

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text: ESALQ/USP

text: public

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characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum spontaneum

text: Eight months

text: Soil

text: Culm

text: Saccharum spontaneum

text: Replicate 3 of sugarcane culm in June:Lane 4


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum spontaneum

characteristic: organism

text: Eight months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum spontaneum

text: Replicate 3 of sugarcane culm in June:Lane 5

characteristic: title


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum spontaneum

text: Eight months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum spontaneum

text: Replicate 3 of sugarcane culm in June:Lane 6


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum spontaneum

text: Twelve months

text: Soil

text: Culm

text: Saccharum spontaneum


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum spontaneum

text: Twelve months

text: Soil

text: Culm

text: Saccharum spontaneum


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

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text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum spontaneum

characteristic: organism

text: Twelve months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum spontaneum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Eight months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar F36-819


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Eight months

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Eight months

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar SP80-3280

characteristic: organism

text: Ten months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in August:Lane 4

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Ten months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in August:Lane 5


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Ten months

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280

text: Replicate 3 of sugarcane culm in August:Lane 6


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum hybrid cultivar R570

text: Ten months

text: Soil

text: Culm

text: Saccharum hybrid cultivar R570


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar R570

characteristic: organism

text: Ten months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar R570

characteristic: title


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar R570

text: Ten months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar R570


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar R570

text: Six months

text: Soil

text: Culm

text: Saccharum hybrid cultivar R570


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum hybrid cultivar R570

text: Six months

text: Soil

text: Culm

text: Saccharum hybrid cultivar R570


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar R570

characteristic: organism

text: Six months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar R570

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Twelve months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar SP80-3280


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Twelve months

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: genotype

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Plants in field conditions

text: Saccharum hybrid cultivar SP80-3280

text: Twelve months

text: Soil

text: Culm

text: Saccharum hybrid cultivar SP80-3280


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ESALQ/USP

text: public

text: Brazil

text: Plants in field conditions

characteristic: isolation and growth condition

text: Saccharum hybrid cultivar F36-819

characteristic: organism

text: Six months

characteristic: plant developmental stage

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ESALQ/USP

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Six months

text: Soil

characteristic: plant growth medium

text: Culm

characteristic: plant structure

text: Saccharum hybrid cultivar F36-819


text: ESALQ/USP

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: common name

text: Brazil

text: Plants in field conditions

text: Saccharum hybrid cultivar F36-819

text: Six months

text: Soil

text: Culm

text: Saccharum hybrid cultivar F36-819


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Berlin Institute for Medical Systems Biology

text: ArrayExpress

characteristic: broker name

text: neuron

characteristic: cell type

text: house mouse

text: Protocols: EBs were cultured in dox-containing monolayer media for five days before separation. 40 hours before separation, EBs on half of the filters were treated with 100nM dTag13 dissolved in DMSO (=sample 40h). Nothing was added to the other half of the filters (=samples 0h). Soma compartment was removed by pipetting and centrifugation (5000g for 1.5min), and adding 400ul of Trifast to soma pellets. The remaining soma was cleaned from the filter using cotton swabs, and the membrane with the remaining neurite compartment was put into 400ul of Trifast (1 filter/replicate, both soma and neurite). RNA was extracted using Phenol/Chloroform extraction, and precipitated using Isopropanol. Concentration was measured using Qubit RNA Assay. 500ng of total RNA isolated from neurites or soma was supplemented with ERCC RNA spike-in mix (4456740 Ambion) and used for library preparation with the Truseq stranded mRNA library prep kit (Illumina 20020594) according to the manufacturer’s recommendation. Libraries were prepared in triplicates (biological replicates).

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

characteristic: progenitor cell type

text: neurite

characteristic: sampling site

text: Mus musculus

unit: hour

characteristic: time


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Berlin Institute for Medical Systems Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: neuron

text: house mouse

text: embryo

text: normal

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

text: embryonic stem cell

text: neurite

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: neuron

characteristic: cell type

text: house mouse

characteristic: common name

text: embryo

text: normal

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: embryonic stem cell

text: soma

text: Mus musculus


text: public

tag: Namespace:Centre for Human Development, Stem Cells and Regeneration Faculty of Medicine University of Southampton

unit: year

characteristic: age

text: ArrayExpress

text: bone marrow cell

text: human

text: Protocols: Excess bone marrow from patients undergoing hip-replacement surgery was used. Samples were stored at 4°C over night and bone marrow mononuclear cells and STRO1+ cells were isolated using FACS as described in Kanczler et al. 2019 (PMID: 30729460). In addition, SNA treated cells were collected from human bone marrow using a FACS Aria cytometer (Becton Dickinson, Wokingham, UK). In total, 20 million cells were sorted from each patient sample. Cells were gated for monocytes, single cells, and Cy5 fluorescence, with the top 20% of fluorescent cells collected. Positive samples were determined to be within the top half of these Cy5 fluorescent cells and negative samples were considered to be within the bottom half of the collected cells. Collected cells were subsequently used for scRNAseq. Single-cell transcriptomes were isolated using the DropSeq protocol described in Macoscko et al. 2015 (PMID:26000488). Protocols for NGS library preparation described in Macosko et al. 2015 (PMID:26000488) were closely followed and pre-amplification was conducted using 4+14 PCR cycles (95°C 3 minutes - 4 cycles of: 98°C 20s; 65°C 45s; 72°C 3min - 14 cycles of: 98°C 20s; 67°C 20s; 72°C 3min - 72°C 5min; 4°C hold).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: SOST+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Centre for Human Development, Stem Cells and Regeneration Faculty of Medicine University of Southampton

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: bone marrow cell

text: human

text: adult

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bone marrow cell

characteristic: cell type

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male

characteristic: title


text: public

characteristic: age

text: ArrayExpress

text: bone marrow cell

text: human

characteristic: common name

text: Protocols: Excess bone marrow from patients undergoing hip-replacement surgery was used. Samples were stored at 4°C over night and bone marrow mononuclear cells were isolated as described in Kanczler et al. 2019 (PMID: 30729460). In addition, whole bone marrow was diluted 1:1 with a-MEM (Gibco) digested with collagenase IV (20U/mL, Thermo Fisher, 17104019) for 30min at 37C under continuous rotation. Where indicated, magnetic cell sorting (MACS) was conducted to enrich or deplete cells. Up to a total of 1x10^8 BM-MNCs were used for each separation. Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec, 130-093-596), CD144 (Miltenyi Biotec, 130-097-857), CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using LS columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions. Single-cell transcriptomes were isolated using the DropSeq protocol described in Macoscko et al. 2015 (PMID:26000488). Protocols for NGS library preparation described in Macosko et al. 2015 (PMID:26000488) were closely followed and pre-amplification was conducted using 4+12 PCR cycles (95°C 3 minutes - 4 cycles of: 98°C 20s; 65°C 45s; 72°C 3min - 12 cycles of: 98°C 20s; 67°C 20s; 72°C 3min - 72°C 5min; 4°C hold).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CD144 negative / CD106 positive

characteristic: immunophenotype

characteristic: individual

characteristic: number of anticipated stamps

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: bone marrow cell

text: human

text: adult

text: normal

text: CD45 negative / CD146 positive

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bone marrow cell

characteristic: cell type

text: human

text: adult

text: normal

text: CD144 positive

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: female

characteristic: title


text: Institute of Epigenetics and Stem Cells, Helmholtz Zentrum Munchen - German Research Center for Environmental Health, Munich, 81377, Germany

text: public

tag: Namespace:Institute of Epigenetics and Stem Cells, Helmholtz Zentrum Munchen – German Research Center for Environmental Health, Munich, 81377, Germany

text: 16 to 19

unit: day

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: The sample was collected in L15 media before briefly being transferred to M2 media (Sigma-Aldrich, Cat No. M7167) for dissection and then dissociated into single cells using 200μl Accutase (ThermoFisher, Cat No. A1110501) for 12 minutes at 37°C, being agitated every 2 minutes, before adding 200μl heat-inactivated FBS (ThermoFisher, Cat No. 10500) to quench the reaction. Cells were then centrifuged at 1000rpm for 3 minutes at 4°C before being suspended in 100μl HBSS (ThermoFisher, Cat No. 14025) + 1% FBS, and stored on ice. Single cells were collected using a Sony SH800 FACS machine with a stringent single-cell collection protocol and sorted into 384 well plates containing SMART-seq2 lysis buffer plus ERCC spike-ins. To ensure good quality cells were collected, a live/dead dye (Abcam, Cat No. ab115347) was used; 100μl was added to the cell suspension at a 2x concentration in HBSS 10 minutes before collection, and live cells were collected based on their FITC intensity. Once cells were collected, plates were sealed, spun down, and frozen using dry ice before being stored at -80°C. This complete process, from dissection to single-cell collection, took approximately 2-3 hours. mRNA from the single embryo was extracted and amplified using the SMARTSeq2 protocol, with the additional inclusion of ERCC spike-in control. Multiplexed sequencing libraries were generated from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: embryo

characteristic: developmental stage

characteristic: individual

text: hemogenic endothelial progenitor

characteristic: inferred cell type - authors labels

text: hemangioblast

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: yolk sac

characteristic: sampling site

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 16 to 19

text: ArrayExpress

text: human

text: embryo

text: yolk sac mesoderm

text: mesenchyme of yolk sac

text: Homo sapiens

text: Homo sapiens

text: whole organism

characteristic: organism part

text: yolk sac

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 16 to 19

text: ArrayExpress

characteristic: broker name

text: human

text: embryo

text: erythrocyte

text: erythrocyte

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: whole organism

text: yolk sac

text: Homo sapiens

text: male

characteristic: title


text: public

text: 16 to 19

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

characteristic: individual

text: endodermal cell

characteristic: inferred cell type - authors labels

text: endodermal cell

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: rostral

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

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characteristic: sampling site

text: Homo sapiens

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 16 to 19

text: ArrayExpress

characteristic: broker name

text: human

text: embryo

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text: mesoderm cell

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characteristic: sex


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text: ArrayExpress

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characteristic: common name

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characteristic: developmental stage

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text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: whole organism

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text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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characteristic: inferred cell type - authors labels

text: epiblast cell

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: rostral

text: Homo sapiens

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: rostral

characteristic: sampling site

text: Homo sapiens

text: male


text: public

text: 16 to 19

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

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text: mesodermal cell

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text: whole organism

characteristic: organism part

text: rostral

text: Homo sapiens

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characteristic: sex


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characteristic: External Id

characteristic: INSDC center name

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text: human

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characteristic: organism

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characteristic: title


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text: hemangioblast

characteristic: inferred cell type - ontology labels

text: Homo sapiens

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text: whole organism

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text: ArrayExpress

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: whole organism

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text: caudal

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characteristic: ENA-CHECKLIST

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characteristic: organism

text: Homo sapiens

text: whole organism

text: caudal

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text: ArrayExpress

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text: Homo sapiens

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

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text: human

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text: Homo sapiens

text: whole organism

text: caudal

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: 16 to 19

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text: human

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text: mesoderm cell

text: Homo sapiens

text: Homo sapiens

text: whole organism

characteristic: organism part

text: caudal

text: Homo sapiens

text: male

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text: public

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text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

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text: embryo

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text: epiblast cell

text: Homo sapiens

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text: Homo sapiens

text: whole organism

text: caudal

text: Homo sapiens

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characteristic: title


characteristic: ENA-LAST-UPDATE

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characteristic: INSDC center name

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text: public

text: 16 to 19

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text: human

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text: erythrocyte

characteristic: inferred cell type - authors labels

text: erythrocyte

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: caudal

text: Homo sapiens

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 16 to 19

text: ArrayExpress

text: human

text: embryo

characteristic: individual

text: endodermal cell

text: endodermal cell

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: rostral

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text: Homo sapiens

text: male


text: public

text: 16 to 19

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text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

text: ectodermal cell

text: ectodermal cell

text: Homo sapiens

text: Homo sapiens

text: whole organism

characteristic: organism part

text: caudal

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

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text: embryo

text: hemogenic endothelial progenitor

text: hemangioblast

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: whole organism

text: caudal

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

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characteristic: Submitter Id

text: 16 to 19

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characteristic: inferred cell type - authors labels

text: mesoderm cell

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

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text: public

text: 16 to 19

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text: ArrayExpress

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text: human

characteristic: common name

characteristic: description

text: embryo

characteristic: developmental stage

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text: yolk sac mesoderm

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text: Homo sapiens

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text: Homo sapiens

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characteristic: ENA-LAST-UPDATE

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text: public

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text: Homo sapiens

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text: ectodermal cell

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text: Homo sapiens

text: Homo sapiens

text: whole organism

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characteristic: ENA-LAST-UPDATE

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text: Homo sapiens

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characteristic: ENA-CHECKLIST

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text: Homo sapiens

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characteristic: ENA-LAST-UPDATE

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text: 16 to 19

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text: embryo

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characteristic: inferred cell type - authors labels

text: primitive streak cell

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text: Homo sapiens

text: Homo sapiens

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text: caudal

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characteristic: ENA-CHECKLIST

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text: whole organism

characteristic: organism part

text: caudal

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

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text: caudal

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text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

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characteristic: SRA accession

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characteristic: inferred cell type - authors labels

text: mesoderm cell

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text: whole organism

text: caudal

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text: public

text: 16 to 19

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

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text: epiblast cell

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: caudal

characteristic: sampling site

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

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text: public

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text: human

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text: whole organism

characteristic: organism part

text: caudal

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

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text: public

characteristic: INSDC status

characteristic: SRA accession

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text: 16 to 19

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text: embryo

text: endodermal cell

text: endodermal cell

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: whole organism

text: caudal

text: Homo sapiens

text: male

characteristic: title


text: public

text: 16 to 19

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: embryo

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text: ectodermal cell

characteristic: inferred cell type - authors labels

text: ectodermal cell

characteristic: inferred cell type - ontology labels

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: caudal

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 16 to 19

text: ArrayExpress

text: human

text: embryo

characteristic: individual

text: hemogenic endothelial progenitor

text: hemangioblast

text: Homo sapiens

text: Homo sapiens

text: whole organism

text: caudal

characteristic: sampling site

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 16 to 19

text: ArrayExpress

text: human

text: embryo

text: nascent mesoderm

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text: Homo sapiens

text: Homo sapiens

text: whole organism

characteristic: organism part

text: yolk sac

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characteristic: sex


E-MTAB-9667:24 h OHT BR1 RPE1

text: public

text: E-MTAB-9667:24 h OHT BR1 RPE1

tag: Namespace:Center for Medical Genetics, Gent, Belgium Cancer Research Institute Gent, Belgium

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cell lines were harvested, centrifuged and stored at -80°C. The parental hTERT-immortalized retinal pigment epithelium (hTERT-RPE1, Clontech, Palo Alto, CA) and RPE1-MYCN-ER were cultured in DMEM/F-12 HEPES, supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 1% L-glutamin and 0,01mg/ml Hygromycin B according to standard procedures. Moreover, 1 ug/ml puromycin was added to the RPE1-MYCN-ER medium composition as a selection marker for the 4-OHT inducible construct. To evaluate transcriptomicchanges RPE1-MYCN-ER and parental hTERT-RPE1 cells were plated in T75 in four biological replicates and treated with or without 400 nM 4-OHT (H6278; Sigma-Aldrich) for 24h, 48 h and 72 h. Controls were supplied with the same amount of EtOH as was present in the treated cells and was always ≤0.5% of the total volume. Total RNA was extracted using miRNeasy kit (Qiagen) according to the manufacturer’s instructions. After RNA extraction, the concentration and quality of the total extracted RNA were checked by using the ‘Quant-it ribogreen RNA assay’ (Life Technologies, Grand Island, NY, USA) and the RNA 6000 Nano chip (Agilent Technologies, Santa Clara, CA, USA), respectively. 400 ng of RNA was used to perform an Illumina sequencing library preparation using the QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen, Vienna, Austria) according to the manufacturer’s protocol. During library preparation, 14 PCR cycles were used. Libraries were quantified by qPCR, according to Illumina’s protocol ‘Sequencing Library qPCR Quantification protocol guide’, version February 2011. A High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA, US) was used to control the library’s size distribution and quality.

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR1 RPE1

characteristic: title


E-MTAB-9667:24 h OHT BR2 RPE1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Center for Medical Genetics, Gent, Belgium Cancer Research Institute Gent, Belgium

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:24 h OHT BR2 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR2 RPE1


E-MTAB-9667:24 h OHT BR3 RPE1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:24 h OHT BR3 RPE1

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR3 RPE1


E-MTAB-9667:24 h OHT BR4 RPE1

text: public

text: E-MTAB-9667:24 h OHT BR4 RPE1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: 24 h OHT BR4 RPE1


E-MTAB-9667:48 h OHT BR1 RPE1

text: public

text: E-MTAB-9667:48 h OHT BR1 RPE1

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR1 RPE1


E-MTAB-9667:48 h OHT BR2 RPE1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:48 h OHT BR2 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR2 RPE1

characteristic: title


E-MTAB-9667:48 h OHT BR3 RPE1

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:48 h OHT BR3 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR3 RPE1


E-MTAB-9667:48 h OHT BR4 RPE1

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:48 h OHT BR4 RPE1

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR4 RPE1


E-MTAB-9667:72 h OHT BR1 RPE1

text: public

text: E-MTAB-9667:72 h OHT BR1 RPE1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: 72 h OHT BR1 RPE1


E-MTAB-9667:72 h OHT BR2 RPE1

text: public

text: E-MTAB-9667:72 h OHT BR2 RPE1

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR2 RPE1


E-MTAB-9667:72 h OHT BR3 RPE1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:72 h OHT BR3 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR3 RPE1

characteristic: title


E-MTAB-9667:72 h OHT BR4 RPE1

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:72 h OHT BR4 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR4 RPE1


E-MTAB-9667:CTRL BR1 RPE1

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:CTRL BR1 RPE1

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR1 RPE1


E-MTAB-9667:CTRL BR2 RPE1

text: public

text: E-MTAB-9667:CTRL BR2 RPE1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: CTRL BR2 RPE1


E-MTAB-9667:CTRL BR3 RPE1

text: public

text: E-MTAB-9667:CTRL BR3 RPE1

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR3 RPE1


E-MTAB-9667:CTRL BR4 RPE1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:CTRL BR4 RPE1

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR4 RPE1

characteristic: title


E-MTAB-9667:24 h OHT BR1 RPE1-MYCN-ER

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:24 h OHT BR1 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR1 RPE1-MYCN-ER


E-MTAB-9667:24 h OHT BR2 RPE1-MYCN-ER

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:24 h OHT BR2 RPE1-MYCN-ER

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR2 RPE1-MYCN-ER


E-MTAB-9667:24 h OHT BR3 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:24 h OHT BR3 RPE1-MYCN-ER

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: 24 h OHT BR3 RPE1-MYCN-ER


E-MTAB-9667:24 h OHT BR4 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:24 h OHT BR4 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: MYCN-ER expression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 24 h OHT BR4 RPE1-MYCN-ER


E-MTAB-9667:48 h OHT BR1 RPE1-MYCN-ER

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:48 h OHT BR1 RPE1-MYCN-ER

text: ArrayExpress

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text: MYCN-ER expression

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characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR1 RPE1-MYCN-ER

characteristic: title


E-MTAB-9667:48 h OHT BR2 RPE1-MYCN-ER

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:48 h OHT BR2 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR2 RPE1-MYCN-ER


E-MTAB-9667:48 h OHT BR3 RPE1-MYCN-ER

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:48 h OHT BR3 RPE1-MYCN-ER

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 48 h OHT BR3 RPE1-MYCN-ER


E-MTAB-9667:48 h OHT BR4 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:48 h OHT BR4 RPE1-MYCN-ER

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

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text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: 48 h OHT BR4 RPE1-MYCN-ER


E-MTAB-9667:72 h OHT BR1 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:72 h OHT BR1 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: MYCN-ER expression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR1 RPE1-MYCN-ER


E-MTAB-9667:72 h OHT BR2 RPE1-MYCN-ER

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:72 h OHT BR2 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR2 RPE1-MYCN-ER

characteristic: title


E-MTAB-9667:72 h OHT BR3 RPE1-MYCN-ER

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:72 h OHT BR3 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR3 RPE1-MYCN-ER


E-MTAB-9667:72 h OHT BR4 RPE1-MYCN-ER

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9667:72 h OHT BR4 RPE1-MYCN-ER

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: 72 h OHT BR4 RPE1-MYCN-ER


E-MTAB-9667:CTRL BR1 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:CTRL BR1 RPE1-MYCN-ER

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cells

characteristic: cell type

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

characteristic: organism part

text: Homo sapiens

text: CTRL BR1 RPE1-MYCN-ER


E-MTAB-9667:CTRL BR2 RPE1-MYCN-ER

text: public

text: E-MTAB-9667:CTRL BR2 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: MYCN-ER expression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR2 RPE1-MYCN-ER


E-MTAB-9667:CTRL BR3 RPE1-MYCN-ER

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9667:CTRL BR3 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR3 RPE1-MYCN-ER

characteristic: title


E-MTAB-9667:CTRL BR4 RPE1-MYCN-ER

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9667:CTRL BR4 RPE1-MYCN-ER

text: ArrayExpress

text: epithelial cells

text: human

text: normal

text: MYCN-ER expression

text: Homo sapiens

text: Homo sapiens

text: pigmented layer of retina

text: Homo sapiens

text: CTRL BR4 RPE1-MYCN-ER


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Austria

characteristic: geographic location (country and/or sea)

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: South Africa

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


characteristic: ENA-CHECKLIST

text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Sweden

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

text: Chad

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

characteristic: environmental sample

text: Latvia

characteristic: geographic location (country and/or sea)

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Israel

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

text: Global sewage consortium

text: Yes

text: France

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

text: United Kingdom

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

characteristic: environmental sample

text: Guatemala

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Hungary

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Madagascar

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Croatia

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Spain

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Germany

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Czech Republic

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Iceland

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: USA

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Malaysia

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Greece

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Portugal

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Saudi Arabia

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Slovenia

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Thailand

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Brazil

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Japan

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Malta

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Turkey

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: India

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Tanzania

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: DTU-GE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Cote d'Ivoire

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: South Korea

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Belgium

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Belgium

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

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text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: USA

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


text: DTU-GE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Uruguay

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Cambodia

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Madagascar

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Uganda

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


text: DTU-GE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Mauritius

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

text: Yes

text: Italy

text: No

text: wastewater

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characteristic: organism

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Taiwan

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: China

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Botswana

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: No

text: wastewater

characteristic: isolation source non-host-associated

text: wastewater metagenome

text: wastewater metagenome

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

text: Global sewage consortium

text: Yes

text: South Africa

text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

characteristic: collected by

characteristic: collection date

text: Yes

characteristic: environmental sample

text: Pakistan

characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

characteristic: INSDC center name

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text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Bolivia

text: No

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: wastewater

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characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

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text: Global sewage consortium

text: Yes

text: Switzerland

text: No

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text: wastewater metagenome

characteristic: strain


text: DTU-GE

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: Global sewage consortium

text: Yes

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text: No

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text: DTU-GE

text: public

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characteristic: broker name

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characteristic: environmental sample

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characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

text: public

text: DTU-GE

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characteristic: geographic location (country and/or sea)

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text: wastewater

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characteristic: organism

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text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

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characteristic: ENA-LAST-UPDATE

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characteristic: strain


text: DTU-GE

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

characteristic: collected by

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characteristic: environmental sample

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text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

text: public

text: DTU-GE

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characteristic: geographic location (country and/or sea)

text: No

text: wastewater

text: wastewater metagenome

characteristic: organism

text: wastewater metagenome


text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

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text: No

characteristic: is the sequenced pathogen host associated?

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characteristic: ENA-LAST-UPDATE

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characteristic: isolation source non-host-associated

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characteristic: strain


text: DTU-GE

characteristic: INSDC last update

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characteristic: geographic location (latitude)

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characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

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text: DTU-GE

characteristic: broker name

text: Global sewage consortium

characteristic: collected by

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characteristic: environmental sample

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text: wastewater

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characteristic: INSDC center name

characteristic: INSDC first public

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characteristic: geographic location (country and/or sea)

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characteristic: is the sequenced pathogen host associated?

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characteristic: INSDC status

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characteristic: ENA-FIRST-PUBLIC

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characteristic: SRA accession

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text: wastewater

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text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

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characteristic: environmental sample

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text: wastewater

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text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Italy

characteristic: geographic location (country and/or sea)

text: No

characteristic: is the sequenced pathogen host associated?

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characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Finland

text: No

characteristic: isolate

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characteristic: organism

text: wastewater metagenome


text: DTU-GE

characteristic: INSDC last update

text: public

characteristic: INSDC status

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text: Global sewage consortium

text: Yes

text: Canada

text: No

text: wastewater

characteristic: isolation source non-host-associated

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characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

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characteristic: SRA accession

characteristic: Submitter Id

text: DTU-GE

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text: Yes

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text: No

text: wastewater

text: wastewater metagenome

text: wastewater metagenome


text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

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text: Yes

characteristic: environmental sample

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text: No

text: wastewater

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text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Argentina

characteristic: geographic location (country and/or sea)

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characteristic: is the sequenced pathogen host associated?

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text: DTU-GE

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characteristic: isolate

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text: DTU-GE

characteristic: INSDC last update

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characteristic: INSDC status

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text: wastewater

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characteristic: ENA-FIRST-PUBLIC

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characteristic: SRA accession

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text: wastewater

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text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

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text: wastewater

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text: DTU-GE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: New Zealand

characteristic: geographic location (country and/or sea)

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characteristic: is the sequenced pathogen host associated?

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characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: DTU-GE

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text: DTU-GE

text: Global sewage consortium

text: Yes

text: Denmark

text: No

characteristic: isolate

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text: DTU-GE

characteristic: INSDC last update

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characteristic: INSDC status

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text: Yes

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text: No

text: wastewater

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characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

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characteristic: SRA accession

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text: Global sewage consortium

text: Yes

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text: No

text: wastewater

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text: DTU-GE

text: public

text: DTU-GE

characteristic: broker name

text: Global sewage consortium

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characteristic: environmental sample

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text: No

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characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: DTU-GE

text: Global sewage consortium

text: Yes

text: Malaysia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: No

characteristic: is the sequenced pathogen host associated?

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characteristic: INSDC last update

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characteristic: INSDC status

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text: Yes

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characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: DTU-GE

text: public

characteristic: SRA accession

characteristic: Submitter Id

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text: Global sewage consortium

text: Yes

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characteristic: isolation source non-host-associated

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characteristic: strain


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characteristic: INSDC center name

characteristic: INSDC first public

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characteristic: broker name

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text: DTU-GE

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text: Global sewage consortium

text: Yes

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characteristic: geographic location (country and/or sea)

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characteristic: is the sequenced pathogen host associated?

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characteristic: strain


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text: DTU-GE

characteristic: broker name

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text: Yes

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text: Cote d'Ivoire

characteristic: geographic location (country and/or sea)

text: No

characteristic: is the sequenced pathogen host associated?

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DTU_2018_950_1_Negative Control_NC_AH

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DTU_2018_951_1_Negative Control_NC_AI

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text: Global sewage consortium

text: Yes

text: Denmark

text: No

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DTU_2018_952_1_Negative Control_NC_AJ

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DTU_2018_954_1_Negative Control_NC_AL

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text: DTU-GE

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text: Denmark

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text: No

characteristic: is the sequenced pathogen host associated?

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text: wastewater

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characteristic: environmental sample

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characteristic: ENA-CHECKLIST

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text: rice

characteristic: common name

text: Oryza sativa

text: Oryza sativa

characteristic: title


E-MTAB-9679:SL1344 1 hour (1)

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text: public

characteristic: INSDC status

text: E-MTAB-9679:SL1344 1 hour (1)

tag: Namespace:Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham

text: ArrayExpress

text: Protocols: 4 biological replicates of each strain were grown for 5 hours. At 1 hour of growth, 6 mL of culture (for each replicate) was centrifuged and cells were harvested and snap frozen. At 3 hours and 5 hours of growth, 1 mL of culture for each replicate was centrifuged and harvested, with snap freezing of the pellets. All centrifugation steps were at 3500 x g and samples were stored at -80°C. Strains were grown in MOPs minimal media (supplemented with histidine) for 5 hours at 37°C with shaking at 150 RPM. Strains were grown from a 4% inoculum of an overnight culture. GENEWIZ Inc. carried out the RNA extraction. Total RNA was extracted from cell pellets using RNeasy Plus Universal kit (Qiagen).To remove rRNA, the ribozero Removal Kit was used (Illumina).RNA quality control was carried out using Qubit 2.0 Fluorometer to measure total RNA concentration and Agilent TapeStation. Library construction was done by GENEWIZ Inc. The NEBNext Ultra II RNA Library Prep Kit (Illumina) was used for library preparation, following the manufacturer’s protocol. For library preparation, cDNA was synthesised, end repaired and adenylated at the 3’ ends. Universal adapters were ligated to cDNA and library enrichment was carried out using limited cycle PCR.

characteristic: description

text: wild type genotype

characteristic: genotype

text: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

characteristic: organism

text: whole organism

characteristic: organism part

text: SL1344 1 hour (1)


E-MTAB-9679:SL1344 1 hour (2)

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text: Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham

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text: public

characteristic: SRA accession

text: E-MTAB-9679:SL1344 1 hour (2)

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: whole organism

text: SL1344 1 hour (2)


E-MTAB-9679:SL1344 1 hour (3)

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text: E-MTAB-9679:SL1344 1 hour (3)

text: ArrayExpress

characteristic: broker name

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text: whole organism

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text: SL1344 1 hour (3)


E-MTAB-9679:SL1344 1 hour (4)

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text: public

text: E-MTAB-9679:SL1344 1 hour (4)

text: ArrayExpress

text: wild type genotype

text: whole organism

text: SL1344 1 hour (4)


E-MTAB-9679:SL1344 3 hour (1)

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text: E-MTAB-9679:SL1344 3 hour (1)

text: ArrayExpress

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text: SL1344 3 hour (1)

characteristic: title


E-MTAB-9679:SL1344 3 hour (2)

characteristic: INSDC last update

text: public

text: E-MTAB-9679:SL1344 3 hour (2)

text: ArrayExpress

text: wild type genotype

text: whole organism

text: SL1344 3 hour (2)


E-MTAB-9679:SL1344 3 hour (3)

text: public

characteristic: INSDC status

text: E-MTAB-9679:SL1344 3 hour (3)

text: ArrayExpress

text: wild type genotype

text: whole organism

text: SL1344 3 hour (3)