characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_M5

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


status: PUBLIC

tag: attribute

text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_M6

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_M7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_M8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_M9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N21

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_N7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_N8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_N9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O15

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O24

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_O7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_O8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_O9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P15

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P16

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P18

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P19

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P20

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P21

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P23

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P24

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P3

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P4

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P6

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P51_male_testis_P7

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P51_male_testis_P8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P51_male_testis_P9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A10

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A12

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A13

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A15

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A16

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A18

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A19

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A20

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A21

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A23

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A24

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A3

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A4

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A6

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A7

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_A9

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B10

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B12

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B13

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B15

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B16

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B18

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B19

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B20

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B21

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B23

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B24

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B3

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B4

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B6

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B7

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_B9

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C10

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C12

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C13

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C15

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C16

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C18

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C19

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C20

text: ArrayExpress

text: fruit fly

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C21

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C22

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C23

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C24

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_C3

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_M8

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_M5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_M9

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: insect proboscis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_N10

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_N11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_N12

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: insect proboscis

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_N13

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P23_male_proboscis_N14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: insect proboscis

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D12

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D13

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D14

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

text: Protocols: Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing. ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D15

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D16

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D17

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D18

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D19

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D20

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D22

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

characteristic: SRA accession

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK


text: BCM

text: public

text: E-MTAB-106281625060381:FCA_P47_male_trachea_D24

text: ArrayExpress

text: fruit fly

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany

text: ArrayExpress

text: Protocols: B. subtilis 168 wild type and the cspB cspD double mutant GP1971 were grown in LB medium to an OD600 of 0.2 and were harvested by mixing them 5:3 with frozen killing buffer (20 mM Tris-HCl pH 7.5, 6 mM MgCl2, 20 mM NaN3) followed by snap-freezing of the pellets in liquid nitrogen. B. subtilis 168 wild type and the cspB cspD double mutant GP1971 were grown in LB medium to an OD600 of 0.2. Total RNA was isolated by acid-phenol extraction. The RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA Nano 6000 kit using an Agilent 2100 Bioanalyzer (Agilent Tech-nologies, Böblingen, Germany). Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA) was used to remove the rRNA. TruSeq Stranded mRNA Library Prep Kit from Illumina was applied to prepare the cDNA libraries.

text: Bacillus subtilis subsp. subtilis str. 168

text: wild type


text: Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: description

text: wild type


text: public

text: ArrayExpress

characteristic: organism

text: GP1971 genotype trpC2 ΔcspB::cat ΔcspD::aphA3


text: UNIVERSITY OF BRISTOL

text: public

tag: Namespace:UNIVERSITY OF BRISTOL

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Bos taurus

characteristic: host scientific name

text: Near-dairy cattle environment

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli

text: Cattle E. coli


text: UNIVERSITY OF BRISTOL

text: public

text: United Kingdom

text: Homo sapiens

text: Human urine

text: Escherichia coli

text: Escherichia coli

text: Urine E. coli

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF BRISTOL

text: public

text: United Kingdom

text: Homo sapiens

text: Human blood

text: Escherichia coli

text: Escherichia coli

text: Bloodsteam E. coli


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF BRISTOL

text: public

text: United Kingdom

text: Canis lupus familiaris

text: Canine faeces

text: Escherichia coli

text: Escherichia coli

text: Canine E. coli


characteristic: ENA-LAST-UPDATE

text: BCM

text: public

text: E-MTAB-106281625070374:FCA_P47_male_trachea_D2

characteristic: age

text: ArrayExpress

text: fruit fly

characteristic: common name

text: adult

characteristic: developmental stage

text: Btl-GAL4; UAS-lamGFP

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: pass

characteristic: post analysis single cell quality

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: single cell identifier

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: External Id

text: BCM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-106281625070374:FCA_P47_male_trachea_D5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: fruit fly

characteristic: description

text: adult

text: Btl-GAL4; UAS-lamGFP

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

text: pass

text: Drosophila melanogaster

text: male

text: OK