characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WETSUS

characteristic: INSDC center name

text: public

characteristic: common name

characteristic: compostmaturity

text: NO

characteristic: compoststerilization

characteristic: description

text: soil

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

text: Netherlands

text: host-associated

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina MiSeq

text: YES

characteristic: soilsterilization


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

text: WETSUS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: YES

text: soil

text: soil

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

characteristic: investigation type

text: uncultured bacterium

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: YES

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WETSUS

characteristic: INSDC center name

text: public

characteristic: common name

characteristic: compostmaturity

text: NO

characteristic: compoststerilization

characteristic: description

text: soil

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

text: Netherlands

text: host-associated

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina MiSeq

text: NO

characteristic: soilsterilization


characteristic: ENA-FIRST-PUBLIC

text: WETSUS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: YES

text: soil

text: soil

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: uncultured bacterium

text: uncultured bacterium

text: Illumina MiSeq

text: NO


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WETSUS

characteristic: INSDC center name

text: public

text: S

characteristic: common name

text: Soil

characteristic: compostmaturity

text: Control

characteristic: compoststerilization

text: S

characteristic: description

text: soil

text: soil

text: soil

text: Netherlands

text: host-associated

characteristic: investigation type

text: uncultured bacterium

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: NO

text: S

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: WETSUS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: st.S

text: Soil

text: Control

text: st.S

text: soil

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Netherlands

text: host-associated

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina MiSeq

text: YES

characteristic: soilsterilization

text: st.S


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WETSUS

characteristic: INSDC center name

text: public

text: st.S+ st.C0

characteristic: common name

characteristic: compostmaturity

text: YES

characteristic: compoststerilization

text: st.S+ st.C0

characteristic: description

text: soil

text: soil

text: soil

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: uncultured bacterium

text: uncultured bacterium

text: Illumina MiSeq

text: YES

text: st.S+ st.C0


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF QUEENSLAND

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF QUEENSLAND

characteristic: Submitter Id

text: Genomic DNA isolated from E. coli strain BW25113 delta yhcB, grown on LB agar supplemented with vancomycin at 37 degrees C, to select for natural revertant suppressor mutants. The data are aligned to the BW25113 reference genome available at NCBI accession CP009273.

text: Escherichia coli

text: Escherichia coli

characteristic: strain

text: Escherichia coli strain BW25113 delta yhcB suppressor mutant

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF QUEENSLAND

characteristic: INSDC center name

text: public

text: Whole genome sequencing data for Keio-yhcB isolate number 1. The data are aligned to the BW25113 reference genome available at NCBI accession CP009273.

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: Escherichia coli strain BW25113 keio-yhcB2


text: UNIVERSITY OF QUEENSLAND

text: public

characteristic: description

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli strain BW25113 keio-yhcB1


BWyhcB

text: UNIVERSITY OF QUEENSLAND

text: public

text: BWyhcB

text: Whole genome sequencing data of E. coli strain BW25113, with the yhcB gene replaced by a kanamycin cassette and excised by recombination of FRT sites leaving a 102 nt scar. The data are aligned to the BW25113 reference genome available at NCBI accession CP009273.

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli strain BW25113 delta yhcB


E-MTAB-10180:Sample 14

text: EPFL

text: public

text: E-MTAB-10180:Sample 14

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

unit: week

characteristic: age

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

text: adipose tissue derived mesenchymal stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Isolation of the mouse stromal vascular fraction (SVF) Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into PBS. The tissues were subsequently minced using scissors, transferred into collagenase (Sigma-Aldrich #C6885-1G, 2 mg/ml of homemade collagenase buffer (25 mM NaHCO3, 12 mM KH2PO4, 1.2 m MgSO4, 4.8 mM KCl, 120 mM NaCl, 1.4 M CaCl2, 5 mM Glucose, 2.5% BSA, pH = 7.4)) and incubated for 65-70 min at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45-50 min of digestion. The cell suspension was then diluted with PBS (with the aim of diluting the collagenase and stopping/slowing down the digestion), and filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell suspension. Next, the cells were pelleted by a 5 min centrifugation at 400 g at room temperature and red blood cells were lysed by incubating the pellet with a homemade red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for a maximum of 5 min, followed by two washes (a 5 min centrifugation at 400 g, room temperature) with a homemade FACS buffer (PBS with 3% foetal bovine serum (FBS) (Gibco #10270-106), 1 mM EDTA, 1% penicillin-streptomycin (Gibco #15140122)). FACS-based cell isolation The SVF cell suspension was diluted to 1 × 10^7 cells/ml with a homemade FACS buffer (see above) and the following fluorophore-conjugated antibodies were added (in titration-determined quantities, Suppl. Table 3): anti-mouse CD31-AF488, anti-mouse CD45-AF488, anti-mouse TER119-AF488 (BioLegend #303110, #304017 and #116215, respectively) to select the Lin− population; anti-mouse SCA1-PE-Cy7 (BioLegend #122513) to enrich the Lin− population with ASPCs; and four different anti-mouse CD142 antibodies: 1) anti-mouse CD142 SinoBiological (SB), monoclonal rabbit IgG clone #001, PE-conjugated, #50413-R001-PE, 2) SinoBiological, monoclonal rabbit IgG clone #001, #50413-R001, in-house conjugated to Lightning-Link PE with Conjugation Kit (Innova Biosciences #703-0010) (SB-LL), 3) BiOrbyt, monoclonal mouse Igg clone HTF-1, PE-conjugated, # ORB507485 (BO), 4) R&D Systems, polyclonal goat IgG, PE-conjugated, #FAB3178P (RnD). The cells were incubated with the antibodies on ice for at least 30 min and protected from light, after which they were washed with the FACS buffer, stained with propidium iodide (Molecular Probes #P3566) for assessing viability, filtered through a 40-µm cell strainer and subjected to FACS with the use of a Becton Dickinson FACSAria II sorter. Compensation measurements were performed for single stains using positive/negative compensation beads (eBiosciences #01-2222-42). The following gating strategy was applied while sorting the cells: first, the cells were selected based on their size and granularity/complexity (side and forward scatter), followed by a double elimination of any events that could represent more than one cell. Next, the Lin−(CD31− CD45− TER119-) population was selected, followed by a Lin−SCA1+ selection (ASPCs), and finally, ASPC cells that were negative or positive for CD142 marker: Lin− SCA1+ CD142− (CD142− ASPCs) and Lin− SCA1+ CD142+ (CD142+ ASPCs, Aregs). Wild-type C57BL/6J male and female adult mice were euthanised at CO2. Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into ice cold PBS for further processing. Equal cell numbers were sorted and plated in flat-bottom microscopy-adapted cell culture plates (Corning #353219), cultured in high glucose DMEM medium (Gibco #61965026) supplemented with 10% FBS and 1% penicillin-streptomycin. At confluence, the cells were induced with white adipogenic differentiation cocktail (see ‘Treatment protocol). In vitro adipogenic differentiation Total, CD142− and CD142+ ASPCs (Aregs) cells isolated with different anti-CD142 antibodies transwell were treated with a single dose of white adipocyte differentiation induction cocktail (0.5 µM 3-isobutyl-1-methylxanthine (IBMX, Sigma #15879), 1 μM dexamethasone (Sigma #D2915), 170 nM (1µg/ml) insulin (Sigma #19278)), followed by a maintenance treatment two-three days post-induction (170 nM insulin) and refreshed every two-three days. The culture was carried out until day six-eight after induction, at which point cells were stained for imaging or collected for RNA extraction. CD142− ASPCs and CD142+ ASPCs (Aregs) isolated with the use of four different ani-CD142 antibodies and having been exposed to the adipogenic differentiation cocktail for 6-8 days, were collected into Tri-Reagent (Molecular Research Center #TR118). Direct-zol RNA kit (Zymo Research #R2052) was used to extract RNA according to the manufacture’s specifications. 3’end bulk mRNA sequencing was performed following the BRB-seq strategy as previously described (Alpern et al., 2019). In brief, 7-200 ng of total RNA from each sample was reverse transcribed in a 96-well plate using SuperScriptTM II Reverse Transcriptase (Lifetech 18064014) with individual barcoded oligo-dT primers, featuring a 12-nt-long sample barcode (IDT). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method. For that, a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E. coli DNA ligase (NEB, #M0205 L), 5 μl of E. coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (10 mM), 10 μl of 5x Second Strand Buffer (100 mM Tris, pH 6.9, (AppliChem, #A3452), 25 mM MgCl2 (Sigma, #M2670), 450 mM KCl (AppliChem, #A2939), 0.8 mM β-NAD (Sigma, N1511), 60 mM (NH4)2SO4 (Fisher Scientific Acros, #AC20587), and 11 μl of water was added to 20 μl of ExoI-treated first-strand reaction on ice. The reaction was incubated at 16 °C for 2.5 h. Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water. The Illumina-compatible libraries were prepared by tagmentation of 10-40 ng of full-length double-stranded cDNA with 1 µl of in-house produced Tn5 enzyme (11 μM). After tagmentation, the libraries where purified with DNA Clean and Concentrator kit (Zymo Research #D4014), eluted in 20 µl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of each i5 and i7 Illumina index adapter (IDT) following this program: incubation 72 °C-3 min, denaturation 98 °C-30 s; 15 cycles: 98 °C-10 s, 63 °C-30 s, 72 °C-30 s; final elongation at 72 °C-5 min. The libraries were purified twice with AMPure beads (Beckman Coulter, #A63881) at 0.6x ratio to remove the fragments < 300 nt. The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 (Illumina).

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 14


E-MTAB-10180:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 4

characteristic: Submitter Id

text: CD142 negative

text: over 8

text: BiOrbyt, monoclonal mouse Igg clone HTF-1, PE-conjugated, # ORB507485

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-10180:Sample 9

characteristic: External Id

text: EPFL

characteristic: INSDC center name

text: public

text: E-MTAB-10180:Sample 9

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 9


E-MTAB-10180:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 19

text: CD142 postive

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 19


E-MTAB-10180:Sample 24

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 24

characteristic: Submitter Id

text: CD142 postive

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 24

characteristic: title


E-MTAB-10180:Sample 29

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 29

text: CD142 postive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 29


E-MTAB-10180:Sample 28

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 28

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 28


E-MTAB-10180:Sample 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 33

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 33

characteristic: title


E-MTAB-10180:Sample 38

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 38

text: total ASPCs

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 38


E-MTAB-10180:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 3

text: CD142 negative

text: over 8

text: R&D Systems, polyclonal goat IgG, PE-conjugated, #FAB3178P

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 3


E-MTAB-10180:Sample 37

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 37

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 37

characteristic: title


E-MTAB-10180:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 8

text: CD142 negative

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 8


E-MTAB-10180:Sample 13

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 13

text: CD142 postive

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 13


E-MTAB-10180:Sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 18

characteristic: Submitter Id

text: CD142 postive

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 18

characteristic: title


E-MTAB-10180:Sample 23

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 23

text: CD142 postive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 23


E-MTAB-10180:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 22

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 22


E-MTAB-10180:Sample 27

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 27

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 27

characteristic: title


E-MTAB-10180:Sample 32

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 32

text: total ASPCs

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 32


E-MTAB-10180:Sample 10

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 10

text: CD142 negative

text: over 8

text: SinoBiological, monoclonal rabbit IgG clone #001, #50413-R001, in-house conjugated to Lightning-Link PE with Conjugation Kit (Innova Biosciences #703-0010)

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 10


E-MTAB-10180:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 15

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-10180:Sample 20

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 20

text: CD142 negative

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 20


E-MTAB-10180:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 25

text: CD142 postive

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 25


E-MTAB-10180:Sample 30

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 30

characteristic: Submitter Id

text: CD142 postive

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 30

characteristic: title


E-MTAB-10180:Sample 35

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 35

text: CD142 postive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 35


E-MTAB-10180:Sample 34

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 34

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 34


E-MTAB-10180:Sample 39

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 39

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 39

characteristic: title


E-MTAB-10180:Sample 5

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 5

text: total ASPCs

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 5


E-MTAB-10180:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 21

text: CD142 negative

text: over 8

text: SinoBiological, monoclonal rabbit IgG clone #001, PE-conjugated, #50413-R001-PE

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 21


E-MTAB-10180:Sample 26

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 26

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 26

characteristic: title


E-MTAB-10180:Sample 31

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 31

text: CD142 negative

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 31


E-MTAB-10180:Sample 36

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 36

text: CD142 negative

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 36


E-MTAB-10180:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 12

characteristic: Submitter Id

text: CD142 postive

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 12

characteristic: title


E-MTAB-10180:Sample 17

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 17

text: CD142 postive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 17


E-MTAB-10180:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 2

text: CD142 postive

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 2


E-MTAB-10180:Sample 7

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10180:Sample 7

characteristic: Submitter Id

text: CD142 postive

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-10180:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10180:Sample 1

text: total ASPCs

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 1


E-MTAB-10180:Sample 11

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 11

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: induction of adipocyte differentiation

characteristic: growth condition

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 11


E-MTAB-10180:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-10180:Sample 16

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 16

characteristic: title


E-MTAB-10180:Sample 6

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10180:Sample 6

text: total ASPCs

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: induction of adipocyte differentiation

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 6


E-MTAB-10181:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EPFL

characteristic: INSDC first public

text: public

text: E-MTAB-10181:Sample 1

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: Protocols: Isolation of the mouse stromal vascular fraction (SVF) Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into PBS. The tissues were subsequently minced using scissors, transferred into collagenase (Sigma-Aldrich #C6885-1G, 2 mg/ml of homemade collagenase buffer (25 mM NaHCO3, 12 mM KH2PO4, 1.2 m MgSO4, 4.8 mM KCl, 120 mM NaCl, 1.4 M CaCl2, 5 mM Glucose, 2.5% BSA, pH = 7.4)) and incubated for 65-70 min at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45-50 min of digestion. The cell suspension was then diluted with PBS (with the aim of diluting the collagenase and stopping/slowing down the digestion), and filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell suspension. Next, the cells were pelleted by a 5 min centrifugation at 400 g at room temperature and red blood cells were lysed by incubating the pellet with a homemade red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for a maximum of 5 min, followed by two washes (a 5 min centrifugation at 400 g, room temperature) with a homemade FACS buffer (PBS with 3% foetal bovine serum (FBS) (Gibco #10270-106), 1 mM EDTA, 1% penicillin-streptomycin (Gibco #15140122)). FACS-based cell isolation The SVF cell suspension was diluted to 1 × 10^7 cells/ml with a homemade FACS buffer (see above) and the following fluorophore-conjugated antibodies were added (in titration-determined quantities, Suppl. Table 3): anti-mouse CD31-AF488, anti-mouse CD45-AF488, anti-mouse TER119-AF488 (BioLegend #303110, #304017 and #116215, respectively) to select the Lin− population; anti-mouse SCA1-PE-Cy7 (BioLegend #122513) to enrich the Lin− population with ASPCs; and four different anti-mouse CD142 antibodies: 1) anti-mouse CD142 SinoBiological (SB), monoclonal rabbit IgG clone #001, PE-conjugated, #50413-R001-PE, 2) SinoBiological, monoclonal rabbit IgG clone #001, #50413-R001, in-house conjugated to Lightning-Link PE with Conjugation Kit (Innova Biosciences #703-0010) (SB-LL), 3) BiOrbyt, monoclonal mouse Igg clone HTF-1, PE-conjugated, # ORB507485 (BO), 4) R&D Systems, polyclonal goat IgG, PE-conjugated, #FAB3178P (RnD). The cells were incubated with the antibodies on ice for at least 30 min and protected from light, after which they were washed with the FACS buffer, stained with propidium iodide (Molecular Probes #P3566) for assessing viability, filtered through a 40-µm cell strainer and subjected to FACS with the use of a Becton Dickinson FACSAria II sorter. Compensation measurements were performed for single stains using positive/negative compensation beads (eBiosciences #01-2222-42). The following gating strategy was applied while sorting the cells: first, the cells were selected based on their size and granularity/complexity (side and forward scatter), followed by a double elimination of any events that could represent more than one cell. Next, the Lin−(CD31− CD45− TER119-) population was selected, followed by a Lin−SCA1+ selection (ASPCs), and finally, ASPC cells that were negative or positive for CD142 marker: Lin− SCA1+ CD142− (CD142− ASPCs) and Lin− SCA1+ CD142+ (CD142+ ASPCs, Aregs). Wild-type C57BL/6J male and female adult mice were euthanised at CO2. Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into ice cold PBS for further processing. CD142− ASPCs and CD142+ ASPCs (Aregs) freshly isolated with the use of four different ani-CD142 antibodies were collected into Tri-Reagent (Molecular Research Center #TR118). Direct-zol RNA kit (Zymo Research #R2052) was used to extract RNA according to the manufacture’s specifications. 3’end bulk mRNA sequencing was performed following the BRB-seq strategy as previously described (Alpern et al., 2019). In brief, 7-200 ng of total RNA from each sample was reverse transcribed in a 96-well plate using SuperScriptTM II Reverse Transcriptase (Lifetech 18064014) with individual barcoded oligo-dT primers, featuring a 12-nt-long sample barcode (IDT). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method. For that, a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E. coli DNA ligase (NEB, #M0205 L), 5 μl of E. coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (10 mM), 10 μl of 5x Second Strand Buffer (100 mM Tris, pH 6.9, (AppliChem, #A3452), 25 mM MgCl2 (Sigma, #M2670), 450 mM KCl (AppliChem, #A2939), 0.8 mM β-NAD (Sigma, N1511), 60 mM (NH4)2SO4 (Fisher Scientific Acros, #AC20587), and 11 μl of water was added to 20 μl of ExoI-treated first-strand reaction on ice. The reaction was incubated at 16 °C for 2.5 h. Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water. The Illumina-compatible libraries were prepared by tagmentation of 10-40 ng of full-length double-stranded cDNA with 1 µl of in-house produced Tn5 enzyme (11 μM). After tagmentation, the libraries where purified with DNA Clean and Concentrator kit (Zymo Research #D4014), eluted in 20 µl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of each i5 and i7 Illumina index adapter (IDT) following this program: incubation 72 °C-3 min, denaturation 98 °C-30 s; 15 cycles: 98 °C-10 s, 63 °C-30 s, 72 °C-30 s; final elongation at 72 °C-5 min. The libraries were purified twice with AMPure beads (Beckman Coulter, #A63881) at 0.6x ratio to remove the fragments < 300 nt. The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 (Illumina).

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 1


E-MTAB-10181:Sample 10

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10181:Sample 10

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 10

characteristic: title


E-MTAB-10181:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10181:Sample 2

text: CD142 positive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 2


E-MTAB-10181:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10181:Sample 3

text: CD142 negative

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 3


E-MTAB-10181:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10181:Sample 4

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-10181:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10181:Sample 5

text: CD142 positive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 5


E-MTAB-10181:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10181:Sample 6

text: CD142 negative

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 6


E-MTAB-10181:Sample 7

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10181:Sample 7

characteristic: Submitter Id

text: total ASPCs

characteristic: adipose stem and progenitor cell type

text: over 8

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-10181:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10181:Sample 8

text: CD142 positive

text: over 8

characteristic: anti-cd142 antibody

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 8


E-MTAB-10181:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10181:Sample 9

text: total ASPCs

text: over 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 9


E-MTAB-10182:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 11

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

unit: day

characteristic: age

text: ArrayExpress

text: house mouse

text: Protocols: Isolation of SVF cells from newborn (P0) mice Burgeoning depots of inguinal subcutaneous adipose tissue were dissected from new-born (P0) wild-type C57BL/6J mice and transferred into PBS. The tissues were then transferred into collagenase solution (see above) and incubated for 1 h at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45 min of digestion. The cell suspension was then filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell preparation. Next, the cells were pelleted by a 5-min centrifugation at 400 g at room temperature and a red blood cell lysis was performed by incubating the pellet in a homemade red blood cell lysis buffer (see above) for 5 min, followed by two washes (a 5-min centrifugation at 400 g, room temperature) with a homemade FACS buffer (see above). Isolation of the mouse stromal vascular fraction (SVF) Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female mice of different ages (P16 (16 days post-birth), 4-week-old, 7-week-old, 11-week-old) and transferred into PBS. The tissues were subsequently minced using scissors, transferred into collagenase (Sigma-Aldrich #C6885-1G, 2 mg/ml of homemade collagenase buffer (25 mM NaHCO3, 12 mM KH2PO4, 1.2 m MgSO4, 4.8 mM KCl, 120 mM NaCl, 1.4 M CaCl2, 5 mM Glucose, 2.5% BSA, pH = 7.4)) and incubated for 65-70 min at 37 °C under agitation (130 rpm on a vertical shaker), after which they were resuspended after 45-50 min of digestion. The cell suspension was then diluted with PBS (with the aim of diluting the collagenase and stopping/slowing down the digestion), and filtered through a 100-µm, then 40-μm cell strainer in order to generate a single cell suspension. Next, the cells were pelleted by a 5 min centrifugation at 400 g at room temperature and red blood cells were lysed by incubating the pellet with a homemade red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA) for a maximum of 5 min, followed by two washes (a 5 min centrifugation at 400 g, room temperature) with a homemade FACS buffer (PBS with 3% foetal bovine serum (FBS) (Gibco #10270-106), 1 mM EDTA, 1% penicillin-streptomycin (Gibco #15140122)). FACS-based cell isolation The SVF cell suspension was diluted to 1 × 10^7 cells/ml with a homemade FACS buffer (see above) and the following fluorophore-conjugated antibodies were added (in titration-determined quantities, Suppl. Table 3): anti-mouse CD31-AF488, anti-mouse CD45-AF488, anti-mouse TER119-AF488 (BioLegend #303110, #304017 and #116215, respectively) to select the Lin− population; anti-mouse SCA1-PE-Cy7 (BioLegend #122513) to enrich the Lin− population with ASPCs, and anti-mouse CD142-PE (# ORB507485 (BO). The cells were incubated with the antibodies on ice for at least 30 min and protected from light, after which they were washed with the FACS buffer, stained with propidium iodide (Molecular Probes #P3566) for assessing viability, filtered through a 40-µm cell strainer and subjected to FACS with the use of a Becton Dickinson FACSAria II sorter. Compensation measurements were performed for single stains using positive/negative compensation beads (eBiosciences #01-2222-42). The following gating strategy was applied while sorting the cells: first, the cells were selected based on their size and granularity/complexity (side and forward scatter), followed by a double elimination of any events that could represent more than one cell. Next, the Lin−(CD31− CD45− TER119-) population was selected, followed by a Lin−SCA1+ selection (ASPCs), and finally, ASPC cells that were negative or positive for CD142 marker: Lin− SCA1+ CD142− (CD142− ASPCs) and Lin− SCA1+ CD142+ (CD142+ ASPCs, Aregs). Wild-type C57BL/6J male and female adult mice were euthanised at CO2. Subcutaneous inguinal adipose tissue depots were dissected from wild-type C57BL/6J male and female adult mice and transferred into ice cold PBS for further processing. Total, CD142− and CD142+ ASPCs (Aregs) freshly isolated form the indicated age groups (P0, P16, 4wo, 7wo and 11wo) were collected into Tri-Reagent (Molecular Research Center #TR118). Direct-zol RNA kit (Zymo Research #R2052) was used to extract RNA according to the manufacture’s specifications. 3’end bulk mRNA sequencing was performed following the BRB-seq strategy as previously described (Alpern et al., 2019). In brief, 7-200 ng of total RNA from each sample was reverse transcribed in a 96-well plate using SuperScriptTM II Reverse Transcriptase (Lifetech 18064014) with individual barcoded oligo-dT primers, featuring a 12-nt-long sample barcode (IDT). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method. For that, a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E. coli DNA ligase (NEB, #M0205 L), 5 μl of E. coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (10 mM), 10 μl of 5x Second Strand Buffer (100 mM Tris, pH 6.9, (AppliChem, #A3452), 25 mM MgCl2 (Sigma, #M2670), 450 mM KCl (AppliChem, #A2939), 0.8 mM β-NAD (Sigma, N1511), 60 mM (NH4)2SO4 (Fisher Scientific Acros, #AC20587), and 11 μl of water was added to 20 μl of ExoI-treated first-strand reaction on ice. The reaction was incubated at 16 °C for 2.5 h. Full-length double-stranded cDNA was purified with 30 μl (0.6x) of AMPure XP magnetic beads (Beckman Coulter, #A63881) and eluted in 20 μl of water. The Illumina-compatible libraries were prepared by tagmentation of 10-40 ng of full-length double-stranded cDNA with 1 µl of in-house produced Tn5 enzyme (11 μM). After tagmentation, the libraries where purified with DNA Clean and Concentrator kit (Zymo Research #D4014), eluted in 20 µl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of each i5 and i7 Illumina index adapter (IDT) following this program: incubation 72 °C-3 min, denaturation 98 °C-30 s; 15 cycles: 98 °C-10 s, 63 °C-30 s, 72 °C-30 s; final elongation at 72 °C-5 min. The libraries were purified twice with AMPure beads (Beckman Coulter, #A63881) at 0.6x ratio to remove the fragments < 300 nt. The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 (Illumina).

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 11

characteristic: title


E-MTAB-10182:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 14

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 14


E-MTAB-10182:Sample 23

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 23

text: CD142 negative

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 23


E-MTAB-10182:Sample 26

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 26

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 26

characteristic: title


E-MTAB-10182:Sample 38

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 38

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 38


E-MTAB-10182:Sample 41

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 41

text: CD142 negative

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 41


E-MTAB-10182:Sample 53

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 53

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 53

characteristic: title


E-MTAB-10182:Sample 56

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 56

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 56


E-MTAB-10182:Sample 58

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 58

text: CD142 negative

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 5

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 58


E-MTAB-10182:Sample 60

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 60

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 60

characteristic: title


E-MTAB-10182:Sample 65

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 65

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 6

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 65


E-MTAB-10182:Sample 66

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 66

text: CD142 negative

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 6

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 66


E-MTAB-10182:Sample 20

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 20

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 20

characteristic: title


E-MTAB-10182:Sample 35

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 35

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: juvenile days 30-44

text: wild type genotype

text: pool 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 35


E-MTAB-10182:Sample 50

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 50

text: CD142 negative

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 50


E-MTAB-10182:Sample 64

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 64

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

text: wild type genotype

text: pool 6

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 64

characteristic: title


E-MTAB-10182:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 8

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: juvenile days 30-44

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 8


E-MTAB-10182:Sample 16

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 16

text: CD142 negative

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 16


E-MTAB-10182:Sample 18

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 18

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 18

characteristic: title


E-MTAB-10182:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 2

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 2


E-MTAB-10182:Sample 29

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 29

text: CD142 negative

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 29


E-MTAB-10182:Sample 32

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 32

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 32

characteristic: title


E-MTAB-10182:Sample 44

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 44

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 44


E-MTAB-10182:Sample 47

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 47

text: CD142 negative

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 47


E-MTAB-10182:Sample 5

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 5

characteristic: Submitter Id

text: CD142 negative

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 5

characteristic: title


E-MTAB-10182:Sample 62

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 62

text: CD142 negative

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 6

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 62


E-MTAB-10182:Sample 63

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 63

text: CD142 negative

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 6

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 63


E-MTAB-10182:Sample 12

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 12

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 12

characteristic: title


E-MTAB-10182:Sample 15

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 15

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 15


E-MTAB-10182:Sample 24

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 24

text: CD142 positive

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 24


E-MTAB-10182:Sample 27

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 27

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 27

characteristic: title


E-MTAB-10182:Sample 39

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 39

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 39


E-MTAB-10182:Sample 42

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 42

text: CD142 positive

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 42


E-MTAB-10182:Sample 54

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 54

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 54

characteristic: title


E-MTAB-10182:Sample 57

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 57

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 57


E-MTAB-10182:Sample 59

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 59

text: CD142 positive

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 5

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 59


E-MTAB-10182:Sample 61

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 61

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 5

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 61

characteristic: title


E-MTAB-10182:Sample 21

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 21

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: juvenile days 30-44

text: wild type genotype

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 21


E-MTAB-10182:Sample 36

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 36

text: CD142 positive

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 36


E-MTAB-10182:Sample 51

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 51

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

text: wild type genotype

text: pool 4

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 51

characteristic: title


E-MTAB-10182:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 9

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: juvenile days 30-44

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 9


E-MTAB-10182:Sample 17

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 17

text: CD142 positive

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 17


E-MTAB-10182:Sample 19

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 19

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 19

characteristic: title


E-MTAB-10182:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 3

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 3


E-MTAB-10182:Sample 30

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 30

text: CD142 positive

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 30


E-MTAB-10182:Sample 33

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 33

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 33

characteristic: title


E-MTAB-10182:Sample 45

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 45

text: CD142 positive

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 45


E-MTAB-10182:Sample 48

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 48

text: CD142 positive

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 48


E-MTAB-10182:Sample 6

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 6

characteristic: Submitter Id

text: CD142 positive

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 6

characteristic: title


E-MTAB-10182:Sample 10

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 10

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 10


E-MTAB-10182:Sample 13

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 13

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 13


E-MTAB-10182:Sample 22

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 22

characteristic: Submitter Id

text: Total ASPCs

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 22

characteristic: title


E-MTAB-10182:Sample 25

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 25

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 25


E-MTAB-10182:Sample 37

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 37

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 37


E-MTAB-10182:Sample 40

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 40

characteristic: Submitter Id

text: Total ASPCs

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 40

characteristic: title


E-MTAB-10182:Sample 52

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 52

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 52


E-MTAB-10182:Sample 55

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 55

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 55


E-MTAB-10182:Sample 34

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 34

characteristic: Submitter Id

text: Total ASPCs

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

text: wild type genotype

text: pool 3

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 34

characteristic: title


E-MTAB-10182:Sample 49

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 49

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: juvenile days 30-44

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 49


E-MTAB-10182:Sample 7

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 7

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: juvenile days 30-44

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 7


E-MTAB-10182:Sample 1

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 1

characteristic: Submitter Id

text: Total ASPCs

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-10182:Sample 28

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 28

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 28


E-MTAB-10182:Sample 31

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 31

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 31


E-MTAB-10182:Sample 4

characteristic: ENA-FIRST-PUBLIC

text: EPFL

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10182:Sample 4

characteristic: Submitter Id

text: Total ASPCs

characteristic: adipose stem and progenitor cell type

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse postnatal

text: wild type genotype

text: pool 1

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-10182:Sample 43

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EPFL

text: public

characteristic: INSDC status

text: E-MTAB-10182:Sample 43

text: Total ASPCs

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: mouse postnatal

text: wild type genotype

text: pool 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: subcutaneous adipose tissue

text: Mus musculus

text: male and female

text: Sample 43


E-MTAB-10182:Sample 46

characteristic: ENA-CHECKLIST

text: EPFL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10182:Sample 46

text: Total ASPCs

text: ArrayExpress

text: house mouse

text: mouse postnatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: subcutaneous adipose tissue

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

text: Sample 46