characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: No

text: No

text: No

text: not provided

text: No

text: No

text: No

text: No

text: not provided

text: saliva sample number AT774

characteristic: description

text: Tap

text: Tap

text: Yes

text: Yes

characteristic: elevation

text: Host-associated

text: Animal

text: Animal secretion

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-oral

characteristic: env package

text: saliva

text: Not Hispanic or Latino

characteristic: ethnicity

text: Baseline

text: 2-3 days/week

text: USA:OK:Tulsa

text: No

text: UBERON:oral cavity

text: UBERON:mouth

text: UBERON:mouth

text: Homo sapiens

text: years

text: inches

text: kg

text: No

text: not provided

text: not provided

text: not provided

text: No

text: No

text: No

text: No

text: No

text: No

text: No

text: No

text: No

text: No

text: human oral metagenome

characteristic: organism

text: UCSDMI

text: FALSE

text: TULSA

text: No

characteristic: pregnant

text: White

text: saliva

text: human oral metagenome

text: female

text: No

text: OK

text: 0-1 hour/day

text: Paulus LIBR

text: No

text: No

text: Yes


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:MRC-University of Glasgow Centre for Virus Research

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: External Id

text: MRC-University of Glasgow Centre for Virus Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: Homo sapiens


text: THE UNIVERSITY OF MELBOURNE

text: public

characteristic: SRA accession

tag: Namespace:THE UNIVERSITY OF MELBOURNE

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Homo sapiens


Dsim Port hot F15 R3

text: public

text: Dsim Port hot F15 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F15 R3


Dsim Port hot F15 R4

text: public

text: Dsim Port hot F15 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F15 R4


Dsim Port hot F15 R5

text: public

text: Dsim Port hot F15 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F15 R5


Dsim Port hot F37 R1

text: public

text: Dsim Port hot F37 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F37 R1


Dsim Port hot F37 R2

text: public

text: Dsim Port hot F37 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F37 R2


Dsim Port hot F37 R3

text: public

text: Dsim Port hot F37 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F37 R3


Dsim Port hot F37 R4

text: public

text: Dsim Port hot F37 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F37 R4


Dsim Port hot F37 R5

text: public

text: Dsim Port hot F37 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F37 R5


Dsim Port cold F3 R1+R2

text: public

text: Dsim Port cold F3 R1+R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F3 R1+R2


Dsim Port cold F11 R1

text: public

text: Dsim Port cold F11 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F11 R1


Dsim Port cold F11 R2

text: public

text: Dsim Port cold F11 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F11 R2


Dsim Port cold F11 R3

text: public

text: Dsim Port cold F11 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F11 R3


Dsim Port cold F11 R4

text: public

text: Dsim Port cold F11 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F11 R4


Dsim Port cold F11 R5

text: public

text: Dsim Port cold F11 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F11 R5


Dsim Port cold F21 R1

text: public

text: Dsim Port cold F21 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F21 R1


Dsim Port cold F21 R2

text: public

text: Dsim Port cold F21 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F21 R2


Dsim Port cold F21 R3

text: public

text: Dsim Port cold F21 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F21 R3


Dsim Port cold F21 R4

text: public

text: Dsim Port cold F21 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F21 R4


Dsim Port cold F21 R5

text: public

text: Dsim Port cold F21 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F21 R5


Dsim Port cold F31 R1

text: public

text: Dsim Port cold F31 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F31 R1


Dsim Port cold F31 R2

text: public

text: Dsim Port cold F31 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F31 R2


Dsim Port cold F31 R3

text: public

text: Dsim Port cold F31 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F31 R3


Dsim Port cold F31 R4

text: public

text: Dsim Port cold F31 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F31 R4


Dsim Port cold F31 R5

text: public

text: Dsim Port cold F31 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F31 R5


Dsim Port cold F41 R1

text: public

text: Dsim Port cold F41 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F41 R1


Dsim Port cold F41 R2

text: public

text: Dsim Port cold F41 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F41 R2


Dsim Port cold F41 R3

text: public

text: Dsim Port cold F41 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F41 R3


Dsim Port cold F41 R4

text: public

text: Dsim Port cold F41 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F41 R4


Dsim Port cold F41 R5

text: public

text: Dsim Port cold F41 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F41 R5


Dsim Port cold F51 R1

text: public

text: Dsim Port cold F51 R1

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F51 R1


Dsim Port cold F51 R2

text: public

text: Dsim Port cold F51 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F51 R2


Dsim Port cold F51 R3

text: public

text: Dsim Port cold F51 R3

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F51 R3


Dsim Port cold F51 R4

text: public

text: Dsim Port cold F51 R4

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F51 R4


Dsim Port cold F51 R5

text: public

text: Dsim Port cold F51 R5

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port cold F51 R5


Dsim Port hot F15 R2

text: public

text: Dsim Port hot F15 R2

text: Drosophila simulans

text: Drosophila simulans

text: Dsim Port hot F15 R2


text: CIC BIOGUNE

text: public

tag: Namespace:CIC BIOGUNE

characteristic: collection date

text: goat

text: goat pbmc

characteristic: environment (biome)

text: pbmc

characteristic: environment (feature)

text: pbmc

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: amplicon

characteristic: investigation type

text: Capra hircus

text: goat PBMC microbiome

characteristic: project name

text: Capra hircus

text: pyrosequencing

characteristic: sequencing method

text: goat PBMC amplicon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Division of Virology, Department of Pathology, University of Cambridge

text: ArrayExpress

characteristic: broker name

text: BSR

characteristic: cell line

text: golden hamster

characteristic: common name

text: Protocols: BSR cells were grown on 90-mm dishes to 80% confluency and infected with TMEV at a multiplicity of infection (MOI) of 3, or mock-infected. At 10 hpi, cells were rinsed with 5 ml of warm PBS, flash frozen in liquid nitrogen, and lysed with 400 μl of lysis buffer [20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, 100 μg/ml cycloheximide and 25 U/ml TURBO DNase (Life Technologies)]. The cells were scraped extensively to ensure lysis, collected and triturated ten times with a 25-G needle. Cell lysates were clarified by centrifugation at 15,000 g for 20 min at 4°C and then stored in liquid nitrogen. RNase I was added to the lysates for 45 minutes at room temperature, following which ribosomes and ribosome-protected fragments of RNA were purified by centrifugation through a sucrose cushion. RNA was extracted from the purified ribosomes by proteinase K digestion (enzyme from NEB, reaction at 42 degrees for 30 minutes) followed by hot acidic phenol:chloroform extraction and ethanol precipitation. The methodologies employed were based on the original protocols of Ingolia and colleagues (Ingolia et al., 2009; Ingolia et al., 2012), except library amplicons were constructed using a small RNA cloning strategy (Guo et al., 2010) adapted to Illumina smallRNA v2 to allow multiplexing. Ribosomal RNA was removed using Ribo-Zero Gold rRNA removal kit (Illumina), and remaining RNA was purified by 15% denaturing urea gel purification to select fragments of 35-65nt. Library amplicons were constructed adapters based on the TruSeq small RNA adapters, with an additional seven random nucleotides at the 5′-end of the 3′-adapter.

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: M3 mutant Theiler's encephalomyelitis virus

characteristic: infect

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: kidney

characteristic: organism part

text: Mesocricetus auratus

characteristic: title


text: Division of Virology, Department of Pathology, University of Cambridge

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: BSR

text: golden hamster

text: neonate

text: normal

text: mock

text: Mesocricetus auratus

text: Mesocricetus auratus

text: kidney

text: Mesocricetus auratus

text: mock


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: BSR

text: golden hamster

text: neonate

text: normal

text: wild type Theiler's encephalomyelitis virus

text: Mesocricetus auratus

text: Mesocricetus auratus

text: kidney

text: Mesocricetus auratus

text: WT


characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: BSR

characteristic: cell line

text: golden hamster

characteristic: common name

text: Protocols: BSR cells were grown on 90-mm dishes to 80% confluency and infected with TMEV at a multiplicity of infection (MOI) of 3, or mock-infected. At 10 hpi, cells were rinsed with 5 ml of warm PBS, flash frozen in liquid nitrogen, and lysed with 400 μl of lysis buffer [20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, 100 μg/ml cycloheximide and 25 U/ml TURBO DNase (Life Technologies)]. The cells were scraped extensively to ensure lysis, collected and triturated ten times with a 25-G needle. Cell lysates were clarified by centrifugation at 15,000 g for 20 min at 4°C and then stored in liquid nitrogen. RNase I was added to the lysates for 45 minutes at room temperature, following which ribosomes and ribosome-protected fragments of RNA were purified by centrifugation through a sucrose cushion. RNA was extracted from the purified ribosomes by proteinase K digestion (enzyme from NEB, reaction at 42 degrees for 30 minutes) followed by hot acidic phenol:chloroform extraction and ethanol precipitation. The methodologies employed were based on the original protocols of Ingolia and colleagues (Ingolia et al., 2009; Ingolia et al., 2012), except library amplicons were constructed using a small RNA cloning strategy (Guo et al., 2010) adapted to Illumina smallRNA v2 to allow multiplexing. Ribosomal RNA was removed using Ribo-Zero Gold rRNA removal kit (Illumina), and remaining RNA was purified by 15% denaturing urea gel purification to select fragments of 19-35nt. Library amplicons were constructed using adapters based on the TruSeq small RNA adapters, with an additional seven random nucleotides at the 5′-end of the 3′-adapter and the 3′-end of the 5′-adapter.

text: neonate

text: normal

text: Mesocricetus auratus

text: Mesocricetus auratus

text: kidney

text: Mesocricetus auratus


text: public

text: ArrayExpress

text: BSR

text: golden hamster

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: mock

characteristic: infect

text: Mesocricetus auratus

text: Mesocricetus auratus

text: kidney

text: Mesocricetus auratus


characteristic: INSDC first public

text: public

text: ArrayExpress

text: BSR

text: golden hamster

text: neonate

text: normal

text: SS mutant Theiler's encephalomyelitis virus

text: Mesocricetus auratus

characteristic: organism

text: Mesocricetus auratus

text: kidney

characteristic: organism part

text: Mesocricetus auratus

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: BSR

text: golden hamster

text: neonate

text: normal

text: Mesocricetus auratus

text: Mesocricetus auratus

text: kidney

text: Mesocricetus auratus


E-MTAB-9407:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9407:Sample 1

tag: Namespace:Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

text: 7 to 10

unit: week

characteristic: age

text: ArrayExpress

text: fibroblast

characteristic: cell type

text: house mouse

text: Protocols: Tumors were harvested, collected in RPMI 1640 medium containing 2% FCS, 20mM Hepes (all from Lonza), 1 mg/ml Collagenase P (Roche), 25 μg/ml DNaseI (Applichem) and 0.8U/ml Dispase I (Roche) and dissociated mechanically. Samples were incubated at 37° C for 45 min, with collection of supernatant and resuspension every 15 min. Cell suspensions were filtered and washed in PBS containing 0.5% FCS and 10mM EDTA. Stromal cells were enriched using CD45 and Ter119 MACS microbeads (Miltenyi) to deplete CD45+ hematopoietic cells and Ter119+ erythrocytes. Mice were subcutaneously inoculated into the flank with 2x10^5 B16F10 melanoma cells. On day 7, mice were treated intratumorally with 1x105 FFU r3LCMV-TRP2 or PBS. Analyses of EYFP+ or PDPN+ fibroblastic stromal cells isolated from the tumors were performed on day 11 (day 4 after treatment). EYFP+ or PDPN+ fibroblastic stromal cells from s.c. implanted B16F10 tumors of r3LCMV-TRP2 or PBS control Cxcl13-Cre/ TdTomato EYFP mice were sorted using the BioRad S3 Cell Sorter. Sorted fibroblasts were run on the 10x Chromium (10x Genomics) system. The cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v2 Chemistry)

text: adult

text: Tg(Cxcl13-Cre/TdTomato) x R26-EYFP

characteristic: genotype

text: s.c. implanted B16F10 melanoma cells

characteristic: growth condition

text: CD45- Ter119- mCherry- CD31- PDPN+ and CD45- Ter119- mCherry- CD31- EYFP+

characteristic: immunophenotype

text: 5 mice pooled

characteristic: individual

text: Mus musculus

text: Mus musculus

text: neoplasm

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-9407:Sample 2

text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9407:Sample 2

text: 7 to 10

text: ArrayExpress

characteristic: broker name

text: fibroblast

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: Tg(Cxcl13-Cre/TdTomato) x R26-EYFP

text: s.c. implanted B16F10 melanoma cells

text: 4 mice pooled

text: Mus musculus

characteristic: organism

text: Mus musculus

text: neoplasm

characteristic: organism part

text: Mus musculus

text: female

text: Sample 2

characteristic: title


E-MTAB-9407:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9407:Sample 3

characteristic: Submitter Id

text: 7 to 10

characteristic: age

text: ArrayExpress

text: fibroblast

text: house mouse

text: adult

text: Tg(Cxcl13-Cre/TdTomato) x R26-EYFP

text: s.c. implanted B16F10 melanoma cells

text: 3 mice pooled

text: Mus musculus

text: Mus musculus

text: neoplasm

text: Mus musculus

text: female

text: Sample 3


E-MTAB-9462:Sample 10

text: Medical School of Nantong University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9462:Sample 10

tag: Namespace:Medical School of Nantong University

text: ArrayExpress

text: house mouse

text: Protocols: Clear serum collected from 6 to 7 mice was pooled in a new tube. Centrifugation at 10,000g for 10 min at 4℃ to remove debris, the supernatant was aliquoted and stored at -80℃. Before sEVs isolation, stored serum was thawed on ice and centrifuged at 21,000 g for 15 min at 4℃, and the supernatant was transferred to a new tube. According to the recommended protocol, 125 μl ExoQuick Solution (System Biosciences Inc., Mountain View, CA, USA) was added to 500 μl serum and incubated at 4℃ for 40 min after a brief up and down. After that, the ExoQuick/serum mixture was centrifuged at 1,500 g for 30 min, and the supernatant was removed by aspiration. Another 5 min centrifugation was performed to remove the residue liquid. The exosomes-containing pellet was re-suspended in nuclease-free water or corresponding buffer for RNA extraction. C57BL/6 male mice (8 weeks) were purchased from Shanghai medical laboratory animal center. Acute liver injury (ALI) was induced by a single intraperitoneal (i.p.) injection 0.5 mL/kg body weight of carbon tetrachloride (CCL4) in olive oil in mice. Mice in the control group received injections of olive oil alone. 0, 2 or 7 days later mice were sacrificed to collect blood and liver for further analysis. Chronic liver injury (CLI) was induced by CCL4 in olive oil twice a week intraperitoneally administration for 8 weeks. Mice were sacrificed at 0, 8 or 12 weeks later to collect blood and liver for further analysis. Total RNA was extracted from serum sEVs samples using TRIzol reagent (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. During RNA sample preparation, 50 fmol of synthetic Caenorhabditis elegans (cel)-miR-39-3p was spiked in and used to normalize the technical variation between the samples. A total amount of 2.5 ng RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra small RNA Sample Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. Briefly, First of all, ligated the 3′ SR Adaptor. Mixed 3′ SR Adaptor for Illumina, RNA and Nuclease-Free Water, mixture system after incubation for 2 minutes at 70 degrees in a preheated thermal cycler. Tube was transfer to ice. Then, add 3′ Ligation Reaction Buffer (2X) and 3′ Ligation Enzyme Mix ligate the 3′ SR Adaptor. Incubated for 1 hour at 25°C in a thermal cycler. To prevent adaptor-dimer formation, the SR RT Primer hybridizes to the excess of 3′ SR Adaptor (that remains free after the 3′ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated. The second, ligated the 5′ SR Adaptor. Then, reverse transcription synthetic first chain. Last, PCR amplification and Size Selection. PAGE gel was used to electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.

text: adult

text: acute liver injury

characteristic: disease

text: wild type genotype

characteristic: genotype

text: ALI

characteristic: individual

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 10


E-MTAB-9462:Sample 9

text: Medical School of Nantong University

text: public

text: E-MTAB-9462:Sample 9

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute liver injury

text: wild type genotype

text: ALI

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood serum

characteristic: organism part

text: Mus musculus

text: male

text: Sample 9

characteristic: title


E-MTAB-9462:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical School of Nantong University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9462:Sample 8

text: ArrayExpress

text: house mouse

text: adult

text: acute liver injury-control

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 8


E-MTAB-9462:Sample 7

text: Medical School of Nantong University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9462:Sample 7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: acute liver injury-control

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 7


E-MTAB-9462:Sample 11

text: Medical School of Nantong University

text: public

text: E-MTAB-9462:Sample 11

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute liver injury-recovery

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 11


E-MTAB-9462:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical School of Nantong University

characteristic: INSDC last update

text: public

text: E-MTAB-9462:Sample 12

text: ArrayExpress

text: house mouse

text: adult

text: acute liver injury-recovery

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood serum

characteristic: organism part

text: Mus musculus

text: male

text: Sample 12

characteristic: title


E-MTAB-9462:Sample 3

text: Medical School of Nantong University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9462:Sample 3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: chronic liver injury

characteristic: disease

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 3


E-MTAB-9462:Sample 4

text: Medical School of Nantong University

text: public

text: E-MTAB-9462:Sample 4

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic liver injury

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 4


E-MTAB-9462:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical School of Nantong University

characteristic: INSDC last update

text: public

text: E-MTAB-9462:Sample 1

text: ArrayExpress

text: house mouse

text: adult

text: chronic liver injury-control

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 1


E-MTAB-9462:Sample 2

text: Medical School of Nantong University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9462:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: chronic liver injury-control

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: blood serum

characteristic: organism part

text: Mus musculus

text: male

text: Sample 2

characteristic: title


E-MTAB-9462:Sample 5

text: Medical School of Nantong University

text: public

text: E-MTAB-9462:Sample 5

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic liver injury-recovery

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 5


E-MTAB-9462:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical School of Nantong University

characteristic: INSDC last update

text: public

text: E-MTAB-9462:Sample 6

text: ArrayExpress

text: house mouse

text: adult

text: chronic liver injury-recovery

text: wild type genotype

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: blood serum

text: Mus musculus

text: male

text: Sample 6


text: NIOO-KNAW

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bacteria

text: Yes

characteristic: environmental sample

text: 51� 32' N, E 5� 51' E

characteristic: lat lon

text: soil metagenome

text: soil metagenome


text: NIOO-KNAW

text: public

text: Fungi

characteristic: common name

characteristic: description

text: Yes

text: 51� 32' N, E 5� 51' E

text: soil metagenome

text: soil metagenome

characteristic: title


E-MTAB-9467:DF Day-1

characteristic: ENA-CHECKLIST

text: MAHIDOL UNIVERSITY

text: public

text: E-MTAB-9467:DF Day-1

tag: Namespace:MAHIDOL UNIVERSITY

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: secondary infections with DENV-4

characteristic: clinical history

text: human

text: Protocols: Blood samples were obtained from patients diagnosed with dengue virus (DENV) infection admitted to Varjira, and Thasongyang Hospitals with informed consent. We obtained the peripheral blood mononuclear cell (PBMC) samples from two Thai male adult donors, both diagnosed with DENV-4 infection, with DF and DHF severities and the viral load of 1.76*10^6 and 1.77 * 10^7, respectively. The PBMC samples were collected at the defervescence day as well as two and one days before defervescence, and two weeks after defervescence. The samples were isolated from the heparinized blood samples using Ficoll-gradient centrifugation. Then, the samples were washed with 2% FBS RPMI and stored in 90%FBS DMSO in liquid nitrogen until used. Frozen PBMCs were thawed, resuspended, washed before dead cell removal. Cell suspension was washed again by 1X PBS and resuspended with 0.04% BSA/PBS to achieve the cell concentration of 500 cell/ul, before passing through 40 uM cell strainers twice. For the PBMC of healthy control, the sample was prepared following the same protocols as the samples from the dengue patients. The nucleic acids were extracted using Chromium 3’ Single-cell Controller V2, following the manufacturers' instructions (10x Genomics, v2 chemistry). Single-cell libraries were constructed using Chromium 3’ Single-cell Controller V2 following the provider’s protocol with the expected cell numbers of 5,000 cells per sample. Each cDNA library was tagged with the i7 index system before being quality checked using Agilent High sensitivity D1000 Tapestation and Qubid.

text: adult

characteristic: developmental stage

text: dengue fever

characteristic: disease

text: patient 1

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: one day before defervescence

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex

text: DF Day-1


E-MTAB-9467:DF Day-2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAHIDOL UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9467:DF Day-2

text: ArrayExpress

text: secondary infections with DENV-4

text: human

text: adult

text: dengue fever

text: patient 1

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: two days before defervescence

text: Homo sapiens

text: male

text: DF Day-2


E-MTAB-9467:DF Def

characteristic: ENA-CHECKLIST

text: MAHIDOL UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-9467:DF Def

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: secondary infections with DENV-4

text: human

characteristic: common name

characteristic: description

text: adult

text: dengue fever

text: patient 1

text: Homo sapiens

text: Homo sapiens

text: blood

text: defervescence

text: Homo sapiens

text: male

text: DF Def

characteristic: title


E-MTAB-9467:DF Wk2

text: MAHIDOL UNIVERSITY

text: public

text: E-MTAB-9467:DF Wk2

text: ArrayExpress

characteristic: broker name

text: secondary infections with DENV-4

characteristic: clinical history

text: human

text: adult

characteristic: developmental stage

text: dengue fever

characteristic: disease

text: patient 1

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: two-week convalescence

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex

text: DF Wk2


E-MTAB-9467:DHF Day-1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAHIDOL UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9467:DHF Day-1

text: ArrayExpress

text: secondary infections with DENV-4

text: human

text: adult

text: dengue hemorrhagic fever

text: patient 2

text: Homo sapiens

text: Homo sapiens

text: blood

text: one day before defervescence

text: Homo sapiens

text: male

text: DHF Day-1


E-MTAB-9467:DHF Day-2

characteristic: ENA-CHECKLIST

text: MAHIDOL UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-9467:DHF Day-2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: secondary infections with DENV-4

text: human

characteristic: common name

text: adult

text: dengue hemorrhagic fever

text: patient 2

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: two days before defervescence

text: Homo sapiens

text: male

text: DHF Day-2


E-MTAB-9467:DHF Def

text: MAHIDOL UNIVERSITY

text: public

text: E-MTAB-9467:DHF Def

text: ArrayExpress

characteristic: broker name

text: secondary infections with DENV-4

characteristic: clinical history

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: dengue hemorrhagic fever

characteristic: disease

text: patient 2

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: defervescence

text: Homo sapiens

text: male

text: DHF Def

characteristic: title


E-MTAB-9467:DHF Wk2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAHIDOL UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9467:DHF Wk2

text: ArrayExpress

text: secondary infections with DENV-4

text: human

text: adult

text: dengue hemorrhagic fever

text: patient 2

text: Homo sapiens

text: Homo sapiens

text: blood

text: two-week convalescence

characteristic: sampling time point

text: Homo sapiens

text: male

characteristic: sex

text: DHF Wk2


E-MTAB-9467:Healthy control

characteristic: ENA-CHECKLIST

text: MAHIDOL UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9467:Healthy control

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: normal

text: human

characteristic: common name

text: adult

text: normal

text: Healthy person

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female

text: Healthy control


E-MTAB-9468:Sample 1

text: public

text: E-MTAB-9468:Sample 1

tag: Namespace:Molecular Pathology, Institute of Pathology & Department of Translational Genomics, University of Cologne

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: lung cancer cell

characteristic: cell type

text: human

text: Protocols: Samples were grown in RPMI1640 with 10% FBS and treated as indicted before harvest by trypsin-based detachment for scRNA-seq. Cells were subjected to gel-in-emulsion capturing using the 10x Genomics protocols as single cells with beads containing reverse transcription primers and reagents. cDNA was constructed using primers containing 10x Genomics GEM beads containing Illumina R1 sequence, 10x Barcode, a UMI and poly-dT sequences. Barcoded cDNA was PCR amplified, purified and R2 sequencing primers were added before sequencing.

characteristic: description

text: adult

characteristic: developmental stage

text: lung adenocarcinoma

characteristic: disease

text: EGFR mutant

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: Sample 1


E-MTAB-9468:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Molecular Pathology, Institute of Pathology & Department of Translational Genomics, University of Cologne

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9468:Sample 2

text: ArrayExpress

text: lung cancer cell

text: human

text: adult

text: lung adenocarcinoma

text: EGFR mutant

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-9468:Sample 3

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9468:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: lung cancer cell

text: human

text: adult

text: lung adenocarcinoma

text: EGFR mutant

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: Sample 3


text: STOCKHOLM UNIVERSITY

text: public

tag: Namespace:STOCKHOLM UNIVERSITY

characteristic: collection date

text: 220 MB

characteristic: estimated size

text: Greece

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: growth chamber

characteristic: isolation and growth condition

characteristic: number of replicons

text: Capsella grandiflora

text: young plants

characteristic: plant developmental stage

text: potting soil

characteristic: plant growth medium

text: leaf

characteristic: plant structure

characteristic: ploidy

text: Capsella grandiflora

text: DNA-seq in Capsella grandiflora


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STOCKHOLM UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ATAC-seq analyses in a single Capsella grandiflora population

characteristic: description

text: 220 MB

text: Greece

text: growth chamber

text: Capsella grandiflora

characteristic: organism

text: young plants

text: potting soil

text: leaf

text: Capsella grandiflora

text: ATAC-seq in Capsella grandiflora

characteristic: title


characteristic: ENA-CHECKLIST

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 11 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: regulatory T cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Colons were removed and washed in PBS/BSA. Excess fat and contents were removed from colons then tissues were opened longitudinally and cut into sections. Colon tissue was washed twice for 25 min in RPMI/BSA/EDTA at 37oC in a shaking incubator. Colon tissue was digested with RPMI/FCS/collagenase VIII/DNase1 for 45 minutes at 37oC in a shaking incubator. Tissue digests were passed through a 70 μm filter and washed in PBS/BSA/EDTA. Tissue digests were separated by centrifugation on a 40%/80% Percoll gradient and cells collected at the interface. Single cell suspensions were washed in PBS/BSA/EDTA and stained with fluorochrome-conjugated antibodies in PBS/BSA/EDTA for 20 min at 4 oC in the dark. Cells were washed and resuspended in PBS/BSA/EDTA/DNase1 for FACS sorting. Foxp3+ Tregs were sorted as live CD45+Lineage-CD4+TCRβ+Foxp3-YFP+ cells. Lineage included CD8, TCRγδ, B220, CD11b, CD11c, Gr1. Dead cells were excluded using DAPI. For gene expression analysis by RNA sequencing, small bulks of 300 Treg cells were sorted into 2μL lysis buffer (0.8% Triton X-100 and 2 U/μL RNase inhibitor) in a 96-well plate on ice Sequencing libraries were prepared following the Smart-seq2 protocol (Picelli et al., 2013) with own in-house indexing primers (Lamble et al., 2013)

text: wild type genotype

characteristic: genotype

text: 300 cells from 5 pooled male and female mice

characteristic: individual

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: mixed

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 11 to 14

text: ArrayExpress

text: regulatory T cell

text: house mouse

text: Dgat1 knockout

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: mixed


E-MTAB-9478:Sample 1

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: E-MTAB-9478:Sample 1

tag: Namespace:Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

text: Protocols: C57BL/6 mice were infected with PR8 H1N1 virus and were euthanized on different days post- infection. Organs such as lungs, spleen and mediastinal lymph nodes (mln) were isolated. The same organs from naïve mice were used as controls. Spleen and mln were mashed and passed through a 70µm filter to obtain single cell suspension. Lungs were processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec) according to manufacturer’s instruction. Splenocytes and lung cells were enriched for total B cells using the EasySep Mouse Pan-B Cell Isolation kit (Stemcell Technologies) while whole mln cells were used for downstream processing. The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat. no: 552094, BD Biosciences) and anti-IgD-Pacific Blue (cat. no: 405712, BD Biosciences), and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells, the cells were washed and stained with LIVE/DEAD™ Fixable Aqua Dead Cell Stain (cat. no: L34957, Invitrogen) according to manufacturer’s instruction. Mice were anesthetized with isoflurane and infected through nasal inoculation with 50 TCID50 Influenza A/Puerto Rico/8/34 (PR8) (Molecular clone; H1N1) diluted in HBBS containing 0.1% BSA. A maximum of 10,000 live HA-specific mature B cells (CD3-B220+IgD-rHA+) were sorted and collected in a BD FACSAria fusion or BD FACSAria III (BD Biosciences) cell sorter. Between 1500-10,000 sorted HA-specific mature B cells from individual organs were processed into single cells in a chromium controller (10X genomics) Libraries were generated following standard10x protocol . After amplification of the cDNA, 5´gene expression library and enriched B cell library, with paired heavy and light chain were generated from cDNA of the same cell using Chromium single cell VDJ reagent kit (V1.1 chemistry, 10X genomics).

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

characteristic: immunophenotype

text: mouse D7_1

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-9478:Sample 10

text: Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 10

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 10


E-MTAB-9478:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9478:Sample 11

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_3

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 11


E-MTAB-9478:Sample 12

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 12

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D14_3

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: strain

text: Sample 12

characteristic: title


E-MTAB-9478:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 13

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_3

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 13


E-MTAB-9478:Sample 14

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 14

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_1

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 14


E-MTAB-9478:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 15

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_1

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-9478:Sample 16

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 16

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_1

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 16


E-MTAB-9478:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9478:Sample 17

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 17


E-MTAB-9478:Sample 18

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 18

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_2

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: strain

text: Sample 18

characteristic: title


E-MTAB-9478:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 19

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_2

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 19


E-MTAB-9478:Sample 2

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 2

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_1

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

text: Sample 2


E-MTAB-9478:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 20

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_3

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: strain

text: Sample 20

characteristic: title


E-MTAB-9478:Sample 21

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 21

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_3

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 21


E-MTAB-9478:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9478:Sample 22

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: 50% Live CD3- B220+IgD-GL7+ 50% Live CD3- B220+

text: mouse D0_1

text: PBS

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 22


E-MTAB-9478:Sample 23

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 23

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: immunophenotype

text: mouse D0_2

text: PBS

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 23

characteristic: title


E-MTAB-9478:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 24

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: mouse D0_2

characteristic: individual

text: PBS

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 24


E-MTAB-9478:Sample 3

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 3

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

characteristic: sex

text: Sample 3


E-MTAB-9478:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 4

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

characteristic: immunophenotype

text: mouse D7_2

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-9478:Sample 5

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 5

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_2

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

characteristic: organism part

text: Mus musculus

text: female

text: Sample 5


E-MTAB-9478:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9478:Sample 6

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_1

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 6


E-MTAB-9478:Sample 7

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9478:Sample 7

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D14_1

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-9478:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9478:Sample 8

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

characteristic: individual

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 8


E-MTAB-9478:Sample 9

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9478:Sample 9

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

text: Sample 9


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UFPA

text: public

text: human

text: Characterize the gut microbiome of indigenous and urban individuals from the Brazilian Amazon, a region of enormous cultural, ethnic, and biological diversity.

text: Brazilian Amazon

characteristic: geographic location (region and locality)

text: Gut

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: gut microbiome

characteristic: protocol

text: Homo sapiens

text: Gut microbiome

characteristic: title


text: UFPA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

characteristic: common name

text: Brazilian Amazon

text: gut

text: Homo sapiens

text: gut microbiome

text: Homo sapiens

text: Gut microbiome


text: STOCKHOLM UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: 220 Mb

characteristic: estimated size

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: growth chamber

characteristic: isolation and growth condition

characteristic: number of replicons

text: Capsella grandiflora

text: Young plant

characteristic: plant developmental stage

text: potting soil

characteristic: plant growth medium

text: leaf

characteristic: plant structure

characteristic: ploidy

text: Capsella grandiflora

text: RNA-seq in Capsella grandiflora


E-MTAB-9495:Two hr Control Rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Life Sciences, Bhubaneswar

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-9495:Two hr Control Rep1

tag: Namespace:Institute of Life Sciences, Bhubaneswar

text: ArrayExpress

characteristic: broker name

text: conventional dendritic cell

characteristic: cell type

text: house mouse

text: Protocols: The RNA-seq library preparation was performed for Zbtb10 KD and control cDC1 MutuDCs at 2h and 6h after CpG activation. As we did time kinetics, we included three independent biological replicates to identify the Zbtb10 depletion mediated global transcriptome changes. Cells were lysed using Lysis buffer (LBP, Takara) Followed by removal of genomic DNA, using gDNA eliminator column, the RNA was then ethanol precipitated in a nucleospin column (Takara). The total RNA was eluted in RNase free water and checked for concentration and purity using Nanodrop. For RNA-seq library preparation 2 µg of total RNA was used to isolate mRNA through magnetic beads using mRNA isolation kit (PolyA mRNA isolation Module, NEB) followed by RNA-seq library preparation using mRNA library preparation kit (NEB) strictly following the vendor recommended protocol.

characteristic: description

text: control

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: Two hr Control Rep1

characteristic: title


E-MTAB-9495:Two hr Control Rep2

text: Institute of Life Sciences, Bhubaneswar

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9495:Two hr Control Rep2

characteristic: Submitter Id

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

characteristic: common name

text: control

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Two hr Control Rep2


E-MTAB-9495:Two hr Control Rep3

characteristic: ENA-CHECKLIST

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Two hr Control Rep3

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: control

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Two hr Control Rep3


E-MTAB-9495:Six hr Control Rep1

characteristic: ENA-FIRST-PUBLIC

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Control Rep1

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: control

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Control Rep1


E-MTAB-9495:Six hr Control Rep2

characteristic: ENA-LAST-UPDATE

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Control Rep2

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: control

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Control Rep2


E-MTAB-9495:Six hr Control Rep3

characteristic: External Id

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Control Rep3

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: control

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Control Rep3


E-MTAB-9495:Two hr Zbtb10 KD Rep1

text: Institute of Life Sciences, Bhubaneswar

characteristic: INSDC center name

text: public

text: E-MTAB-9495:Two hr Zbtb10 KD Rep1

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: Zbtb10 knockdown

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Two hr Zbtb10 KD Rep1


E-MTAB-9495:Two hr Zbtb10 KD Rep2

text: Institute of Life Sciences, Bhubaneswar

characteristic: INSDC first public

text: public

text: E-MTAB-9495:Two hr Zbtb10 KD Rep2

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: Zbtb10 knockdown

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Two hr Zbtb10 KD Rep2


E-MTAB-9495:Two hr Zbtb10 KD Rep3

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Two hr Zbtb10 KD Rep3

text: ArrayExpress

characteristic: broker name

text: conventional dendritic cell

text: house mouse

text: Zbtb10 knockdown

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Two hr Zbtb10 KD Rep3


E-MTAB-9495:Six hr Zbtb10 KD Rep1

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Zbtb10 KD Rep1

text: ArrayExpress

text: conventional dendritic cell

characteristic: cell type

text: house mouse

text: Zbtb10 knockdown

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Zbtb10 KD Rep1


E-MTAB-9495:Six hr Zbtb10 KD Rep2

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Zbtb10 KD Rep2

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

characteristic: description

text: Zbtb10 knockdown

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Zbtb10 KD Rep2


E-MTAB-9495:Six hr Zbtb10 KD Rep3

text: Institute of Life Sciences, Bhubaneswar

text: public

text: E-MTAB-9495:Six hr Zbtb10 KD Rep3

text: ArrayExpress

text: conventional dendritic cell

text: house mouse

text: Zbtb10 knockdown

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Six hr Zbtb10 KD Rep3


text: University of Turku, Turku, FINLAND

text: public

tag: Namespace:University of Turku, Turku, FINLAND

text: ArrayExpress

text: keratinocyte

text: house mouse

text: Protocols: Mouse keratinocytes isolated from adult dorsal skin were cultured in the comprised FAD medium: three parts DMEM medium (Lonza), one part Ham’s F12 medium (Thermo Fisher Scientific) supplemented with 10% FCS (Biowest), 2 mM L-glutamine (Lonza), 10 units/ml Pen-Strep (Lonza), 200 µM adenine (Sigma-Aldrich), 0.5μg/ml hydrocortisone (Sigma-Aldrich), 5μg/ml insulin (Sigma-Aldrich), 16,8 ng/ml cholera toxin (Sigma-Aldrich) and 10 ng/ml epidermal growth factor (Sigma-Aldrich. The cells were cultured at 37°C in a humidified atmosphere with 5% CO2. Subconfluent mouse keratinocytes were transfected with 75 nM siRNA (Mm_Emb_1 and Mm_Emb_4 Flexitube siRNAs and AllStars Negative Control siRNA from QIAGEN) by using siLentFect reagent (Bio-Rad) according to manufacturer’s instructions and cultured for 48 h. Total RNA was isolated by using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s instructions. Library preparation was performed according to TruSeq Stranded mRNA Sample Preparation protocol (Illumina, 15031047)and TruSeq Stranded mRNA HT Kit (Illumina)

text: adult

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: dorsal skin

characteristic: organism part

text: Mus musculus


E-MTAB-9491:Sample 1

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 1

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

text: adult

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

characteristic: immunophenotype

text: mouse D7_1

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-9491:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-9491:Sample 10

characteristic: Submitter Id

text: 8 to 12

text: ArrayExpress

characteristic: broker name

text: B cell

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 10


E-MTAB-9491:Sample 11

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 11

text: 8 to 12

characteristic: age

text: ArrayExpress

text: B cell

characteristic: cell type

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_3

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 11


E-MTAB-9491:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 12

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D14_3

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 12

characteristic: title


E-MTAB-9491:Sample 13

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9491:Sample 13

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_3

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 13


E-MTAB-9491:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 14

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_1

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 14


E-MTAB-9491:Sample 15

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 15

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_1

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-9491:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9491:Sample 16

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_1

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 16


E-MTAB-9491:Sample 17

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 17

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_2

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 17


E-MTAB-9491:Sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 18

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_2

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 18

characteristic: title


E-MTAB-9491:Sample 19

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9491:Sample 19

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 19


E-MTAB-9491:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 2

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_1

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

characteristic: organism part

text: Mus musculus

text: female

text: Sample 2


E-MTAB-9491:Sample 20

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 20

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D28_3

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 20

characteristic: title


E-MTAB-9491:Sample 21

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9491:Sample 21

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D28_3

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 21


E-MTAB-9491:Sample 22

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 22

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: mouse D0_1

text: PBS

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 22


E-MTAB-9491:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 23

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

characteristic: immunophenotype

text: mouse D0_2

characteristic: individual

text: PBS

characteristic: infect

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 23

characteristic: title


E-MTAB-9491:Sample 24

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9491:Sample 24

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: mouse D0_2

text: PBS

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

text: Sample 24


E-MTAB-9491:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 3

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_2

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 3


E-MTAB-9491:Sample 4

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 4

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

characteristic: immunophenotype

text: mouse D7_2

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 4

characteristic: title


E-MTAB-9491:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9491:Sample 5

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3-NK1.1- B220+ IgD-HA+

text: mouse D7_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

text: Sample 5


E-MTAB-9491:Sample 6

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9491:Sample 6

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_1

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 6


E-MTAB-9491:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 7

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

text: house mouse

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

characteristic: immunophenotype

text: mouse D14_1

characteristic: individual

text: Influenza A virus PR8

characteristic: infect

text: Mus musculus

text: Mus musculus

text: mediastinal lymph node

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 7

characteristic: title


E-MTAB-9491:Sample 8

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9491:Sample 8

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: intranasal infection

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

text: Influenza A virus PR8

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 8


E-MTAB-9491:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-9491:Sample 9

text: 8 to 12

text: ArrayExpress

text: B cell

text: intranasal infection

characteristic: clinical history

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Live CD3- B220+ IgD- HA+

text: mouse D14_2

text: Influenza A virus PR8

text: Mus musculus

characteristic: organism

text: Mus musculus

text: mediastinal lymph node

characteristic: organism part

text: Mus musculus

text: female

text: Sample 9


text: Xingchun Li

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Xingchun Li

characteristic: collection date

text: root-associated fungi

text: root fragments

text: evergreen broad-leaf forest

characteristic: environment (biome)

text: forest

characteristic: environment (feature)

text: root

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: single amplified genome

characteristic: investigation type

text: root associated fungus metagenome

text: plant-associated

characteristic: plant-associated environmental package

text: root-associated fungal community in a subtropical diverse forest

characteristic: project name

text: root associated fungus metagenome

text: Illumina Miseq

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Microbial ecology

text: public

characteristic: SRA accession

tag: Namespace:Microbial ecology

characteristic: Submitter Id

text: Old surface deposit sample

characteristic: description

text: Dry stormwater detention basin

text: Urban deposits sediments

text: Sediments

text: France

text: metagenome

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: urban metagenome

characteristic: organism

text: DJANGO tpm

text: urban metagenome

text: Illumina MiSeq

text: Urban deposits samples


text: Microbial ecology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Control sample

text: Dry stormwater detention basin

characteristic: environment (biome)

text: Urban deposits sediments

characteristic: environment (feature)

text: Sediments

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

text: metagenome

text: urban metagenome

text: DJANGO tpm

text: urban metagenome

text: Illumina MiSeq

text: Urban deposits samples


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Microbial ecology

text: public

text: Old pit deposit sample

text: Dry stormwater detention basin

text: Urban deposits sediments

text: Sediments

text: France

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: urban metagenome

text: DJANGO tpm

characteristic: project name

text: urban metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: Urban deposits samples

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Microbial ecology

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Recent surface deposit sample

characteristic: description

text: Dry stormwater detention basin

text: Urban deposits sediments

text: Sediments

text: France

text: metagenome

characteristic: miscellaneous environmental package

text: urban metagenome

characteristic: organism

text: DJANGO tpm

text: urban metagenome

text: Illumina MiSeq

text: Urban deposits samples


text: Microbial ecology

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Recent pit deposit sample

text: Dry stormwater detention basin

characteristic: environment (biome)

text: Urban deposits sediments

characteristic: environment (feature)

text: Sediments

characteristic: environment (material)

text: France

characteristic: geographic location (country and/or sea)

text: metagenome

text: urban metagenome

text: DJANGO tpm

text: urban metagenome

text: Illumina MiSeq

text: Urban deposits samples


E-MTAB-6588_2:control 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:control 1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: RNA was harvested by Trizol, DNA contaminations were removed by DNase1 digestion in the presence of Rnase Inhibitor, followed by RNA purification via phenol/chloroform extraction. For RNA Isolation, organoids were transferred to 15ml collection tube and washed once with ice cold PBS. RNA was extracted with Trizol, followed by DNA digestion. We established organoids from the small intestine of NICDEYFPflox/flox/p53flox/flox; VillinCreERT2 mice bred under sterile conditions. Small intestines were dissected and small intestinal crypt were isolated and cultured as 3D-intestinal organoids (as described in Sato et al. Nature 459, 262-265 (2009)). Mutagenesis were induced by 4-OHT-Tamoxifen (400nM for 24h) to generate mutant organoids. Organoids that served as wt controls were not treated with 4-OHT. Mutant organoids were selected by withdrawal of EGF, R-Spondin1 and Noggin. Mutant organoids were cultured in full medium prior comparing with wt-control organoids. Organoids were generated in independent experiments. RNA was harvested by Trizol, DNA contaminations were removed by DNase1 digestion in the presence of Rnase Inhibitor, followed by RNA purification via phenol/chloroform extraction. Sequencing libraries were generated using the Illumina TruSeq Stranded mRNA Kit and sample were processed in two pools with HiSeq 4000 in one run (1 pool - 1 lane).

text: VillinCreERT2;NICD-EYFPflox/flox;p53flox/flox

characteristic: genotype

text: organoid culture

characteristic: growth condition

characteristic: individual

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: wild type

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: control 1

characteristic: title


E-MTAB-6588_2:control 1.1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MDC-Berlin

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6588_2:control 1.1

characteristic: Submitter Id

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

characteristic: description

text: organoid culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

text: wild type

text: Mus musculus

text: male

text: control 1.1


E-MTAB-6588_2:control 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:control 2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: genotype

text: organoid culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: small intestine

text: wild type

text: Mus musculus

text: male

text: control 2


E-MTAB-6588_2:control 2.1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:control 2.1

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

text: organoid culture

characteristic: individual

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: wild type

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: control 2.1

characteristic: title


E-MTAB-6588_2:control 3

text: MDC-Berlin

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6588_2:control 3

characteristic: Submitter Id

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

characteristic: description

text: organoid culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

text: wild type

text: Mus musculus

text: male

text: control 3


E-MTAB-6588_2:control 3.1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:control 3.1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: genotype

text: organoid culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: small intestine

text: wild type

text: Mus musculus

text: male

text: control 3.1


E-MTAB-6588_2:mutant 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:mutant 1

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

text: organoid culture

characteristic: individual

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: loss of p53 and expression of NICD

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

text: 4-hydroxytamoxifen; 400 nanomolar for 24 hours

characteristic: stimulus

characteristic: strain

text: mutant 1

characteristic: title


E-MTAB-6588_2:mutant 1.1

characteristic: ENA-CHECKLIST

text: MDC-Berlin

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6588_2:mutant 1.1

characteristic: Submitter Id

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

characteristic: description

text: organoid culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

text: loss of p53 and expression of NICD

text: Mus musculus

text: female

text: mutant 1.1


E-MTAB-6588_2:mutant 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MDC-Berlin

text: public

text: E-MTAB-6588_2:mutant 2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: genotype

text: organoid culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: small intestine

text: loss of p53 and expression of NICD

text: Mus musculus

text: male

text: mutant 2


E-MTAB-6588_2:mutant 2.1

characteristic: ENA-CHECKLIST

text: MDC-Berlin

characteristic: INSDC center name

text: public

text: E-MTAB-6588_2:mutant 2.1

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

text: organoid culture

characteristic: individual

text: Mus musculus

text: Mus musculus

text: small intestine

characteristic: organism part

text: loss of p53 and expression of NICD

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

characteristic: stimulus

characteristic: strain

text: mutant 2.1

characteristic: title


E-MTAB-6588_2:mutant 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: MDC-Berlin

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-6588_2:mutant 3

characteristic: Submitter Id

text: ArrayExpress

text: intestinal epithelial cell

text: house mouse

characteristic: description

text: organoid culture

text: Mus musculus

characteristic: organism

text: Mus musculus

text: small intestine

text: loss of p53 and expression of NICD

text: Mus musculus

text: male

text: mutant 3


E-MTAB-6588_2:mutant 3-1

characteristic: ENA-CHECKLIST

characteristic: External Id

text: MDC-Berlin

characteristic: INSDC center name

text: public

text: E-MTAB-6588_2:mutant 3-1

characteristic: age

text: ArrayExpress

characteristic: broker name

text: intestinal epithelial cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: genotype

text: organoid culture

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: small intestine

text: loss of p53 and expression of NICD

text: Mus musculus

text: male

text: mutant 3-1


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CENTER FOR AGRICULTURAL BIOTECHNOLOGY

text: public

tag: Namespace:CENTER FOR AGRICULTURAL BIOTECHNOLOGY

characteristic: collection date

text: Developing leaf

text: forest

characteristic: environment (biome)

characteristic: estimated size

characteristic: extrachromosomal elements

text: Thailand

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mae Rewa district, Nakornsawan province

characteristic: geographic location (region and locality)

text: Hugo Volkaert

characteristic: identified by

text: developing leaf

characteristic: isolation and growth condition

characteristic: number of replicons

text: Tectona grandis

text: teak

characteristic: organism common name

text: mature plant

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: hermaphroditic

characteristic: plant sex

text: leaf

characteristic: plant structure

characteristic: ploidy

text: Tectona grandis

text: Teak from Wong river watershed

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CENTER FOR AGRICULTURAL BIOTECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Developing leaf

characteristic: description

text: forest

characteristic: environment (biome)

characteristic: estimated size

text: Thailand

text: Khlong Lan district, Kamphaengphet province

text: Hugo Volkaert

text: developing leaf

text: Tectona grandis

characteristic: organism

text: teak

text: mature plant

text: soil

text: hermaphroditic

text: leaf

text: Tectona grandis

text: Teak from Ping river watershed