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text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: Limnoperna fortunei

text: MALE

text: IB UFRJ 19952

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

text: FÁBIO SENDIM MANOEL

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: TAQUARI RIVER

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: IGOR CHRISTO MIYAHIRA

characteristic: identified by

text: adult

characteristic: lifestage

text: Limnoperna fortunei

text: GILL ANIMAL

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Limnoperna fortunei

text: FEMALE

characteristic: sex

characteristic: specimen id

text: IB UFRJ 19952

characteristic: specimen voucher

text: N

characteristic: symbiont


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: FÁBIO SENDIM MANOEL

characteristic: collected by

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collecting institution

text: Brazil

text: TAQUARI RIVER

text: Freshwater

text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

text: GONAD

text: Limnoperna fortunei

text: FEMALE

text: IB UFRJ 19952

text: N

characteristic: title

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

text: FÁBIO SENDIM MANOEL

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: TAQUARI RIVER

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: IGOR CHRISTO MIYAHIRA

characteristic: identified by

text: adult

characteristic: lifestage

text: Limnoperna fortunei

characteristic: organism

text: **OTHER SOMATIC ANIMAL TISSUE**

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Limnoperna fortunei

text: FEMALE

characteristic: sex

characteristic: specimen id

text: IB UFRJ 19952

characteristic: specimen voucher

text: N


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: FÁBIO SENDIM MANOEL

characteristic: collected by

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collecting institution

text: Brazil

text: TAQUARI RIVER

text: Freshwater

text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

text: INTESTINE

text: Limnoperna fortunei

text: FEMALE

text: IB UFRJ 19952

text: N

characteristic: symbiont

characteristic: title


text: Sanger Institute

text: Tree of Life Programme

text: public

text: FÁBIO SENDIM MANOEL

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: TAQUARI RIVER

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: IGOR CHRISTO MIYAHIRA

characteristic: identified by

text: adult

characteristic: lifestage

text: Limnoperna fortunei

characteristic: organism

text: MUSCLE

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Limnoperna fortunei

text: FEMALE

characteristic: sex

characteristic: specimen id

text: IB UFRJ 19952

text: N

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: FÁBIO SENDIM MANOEL

characteristic: collected by

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collecting institution

text: Brazil

text: TAQUARI RIVER

text: Freshwater

text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

text: GILL ANIMAL

text: Limnoperna fortunei

text: MALE

text: IB UFRJ 19952

characteristic: specimen voucher

text: N

characteristic: symbiont


text: Sanger Institute

text: Tree of Life Programme

text: public

text: FÁBIO SENDIM MANOEL

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: TAQUARI RIVER

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: IGOR CHRISTO MIYAHIRA

characteristic: identified by

text: adult

characteristic: lifestage

text: Limnoperna fortunei

characteristic: organism

text: GONAD

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Limnoperna fortunei

text: MALE

characteristic: sex

characteristic: specimen id

text: IB UFRJ 19952

text: N

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: FÁBIO SENDIM MANOEL

characteristic: collected by

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collecting institution

text: Brazil

text: TAQUARI RIVER

text: Freshwater

text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

text: **OTHER SOMATIC ANIMAL TISSUE**

text: Limnoperna fortunei

text: MALE

text: IB UFRJ 19952

characteristic: specimen voucher

text: N

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

text: FÁBIO SENDIM MANOEL

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collection date

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: TAQUARI RIVER

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: IGOR CHRISTO MIYAHIRA

characteristic: identified by

text: adult

characteristic: lifestage

text: Limnoperna fortunei

characteristic: organism

text: INTESTINE

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Limnoperna fortunei

text: MALE

characteristic: sex

characteristic: specimen id

text: IB UFRJ 19952

text: N

characteristic: symbiont


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: FÁBIO SENDIM MANOEL

characteristic: collected by

text: BIO BUREAU BIOTECNOLOGIA LTDA.

characteristic: collecting institution

text: Brazil

text: TAQUARI RIVER

text: Freshwater

text: IGOR CHRISTO MIYAHIRA

text: adult

text: Limnoperna fortunei

text: MUSCLE

text: Limnoperna fortunei

text: MALE

text: IB UFRJ 19952

characteristic: specimen voucher

text: N

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Ilik Saccheri

text: University of Liverpool

characteristic: collection date

text: pepper-and-salt moth

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Liverpool | University of Liverpool Biosciences Building

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Biston betularia

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Biston betularia

text: FEMALE

characteristic: sex

characteristic: specimen id

text: NOT COLLECTED

text: e74d1b9f-a9df-4ba1-b25e-444dd0d4e4ed-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Ilik Saccheri

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

text: pepper-and-salt moth

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Biston betularia

text: WHOLE ORGANISM

text: DTOL

text: Biston betularia

text: MALE

text: NOT COLLECTED

characteristic: specimen voucher

text: 52cbd6fd-565a-48fb-ade5-e192a71cec45-DTOL-specimen


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Iain Barr

text: University of East Anglia

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Norwich | University of East Anglia campus

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Eilema griseolum

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Eilema griseolum

text: MALE

characteristic: sex

text: NOT COLLECTED

text: f0783691-6ec5-4888-a663-512d20d4a915-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Iain Barr

text: University of East Anglia

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Eilema griseolum

text: WHOLE ORGANISM

text: DTOL

text: Eilema griseolum

text: MALE

characteristic: specimen id

text: NOT COLLECTED

text: 2845ade2-3f1d-4364-8235-13b63569fb8d-DTOL-specimen

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Ilik Saccheri

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: squinting bush brown

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Bicyclus anynana

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: Bicyclus anynana

text: FEMALE

text: NOT COLLECTED

characteristic: specimen voucher

text: f648fb98-6e10-4fe9-8f84-9bf9703ba6af-DTOL-specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Ilik Saccheri

text: University of Liverpool

text: squinting bush brown

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Bicyclus anynana

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Bicyclus anynana

text: MALE

characteristic: sex

text: NOT COLLECTED

text: 2738579c-8c38-477b-a3c0-c3bdb89e64a5-DTOL-specimen

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Ilik Saccheri

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: squinting bush brown

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Bicyclus anynana

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Bicyclus anynana

text: FEMALE

characteristic: specimen id

text: NOT COLLECTED

text: 3a779671-a314-4477-872d-ae000ed34e4a-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Ilik Saccheri

text: University of Liverpool

text: pepper-and-salt moth

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Biston betularia

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

characteristic: sample same as

text: Biston betularia

text: FEMALE

text: NOT COLLECTED

characteristic: specimen voucher

text: 8147eff8-47b2-4427-a80f-b202e0817da0-dtol


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Ilik Saccheri

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: pepper-and-salt moth

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Biston betularia

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Biston betularia

text: MALE

characteristic: sex

characteristic: specimen id

text: NOT COLLECTED

text: c45c72b5-16ad-4145-bbf5-7dc1942c5d50-dtol

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Iain Barr

text: University of East Anglia

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Eilema griseolum

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

characteristic: sample same as

text: Eilema griseolum

text: MALE

text: NOT COLLECTED

text: fc2125a1-f7fb-40c0-a956-61ff8e6e7ce3-dtol

characteristic: tolid


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Iain Barr

characteristic: collected by

text: University of East Anglia

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Eilema griseolum

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Eilema griseolum

text: MALE

characteristic: sex

characteristic: specimen id

text: NOT COLLECTED

characteristic: specimen voucher

text: 3c7fa0b6-b767-4e31-8efd-1b182806c4d6-dtol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Ilik Saccheri

text: University of Liverpool

text: squinting bush brown

characteristic: common name

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Bicyclus anynana

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Bicyclus anynana

text: FEMALE

text: NOT COLLECTED

text: 88fda3dc-2d0d-44a6-b5b8-997183e9ab1e-dtol

characteristic: title


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Ilik Saccheri

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: squinting bush brown

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Bicyclus anynana

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: sample same as

text: Bicyclus anynana

text: MALE

characteristic: sex

characteristic: specimen id

text: NOT COLLECTED

text: b750e3ea-bb2d-4b53-b783-b86a198dae0b-dtol

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Ilik Saccheri

text: University of Liverpool

text: squinting bush brown

characteristic: common name

text: United Kingdom

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Bicyclus anynana

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Bicyclus anynana

text: FEMALE

text: NOT COLLECTED

characteristic: specimen voucher

text: e9178b8e-d1b5-49ae-9c5e-77099379b35b-dtol


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Georgina Palmer

characteristic: collected by

text: University of York

characteristic: collecting institution

characteristic: collection date

text: mountain ringlet

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Irton Fell

characteristic: geographic location (region and locality)

text: mountain grassland

characteristic: habitat

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Erebia epiphron

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Erebia epiphron

text: FEMALE

characteristic: sex

characteristic: specimen id

text: NOT COLLECTED

text: 750f9f8d-bc78-453e-a6fd-1a18e065fbb8-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Samuel Whiteford

text: University of Liverpool

text: United Kingdom

text: England | Liverpool |

text: Urban

text: Samual Whiteford

text: University ofLiverpool

text: larva

text: Luffia ferchaultella

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Luffia ferchaultella

text: FEMALE

text: NOT COLLECTED

text: 199f6d0d-4e68-485e-9d1b-a84237b84afa-DTOL-specimen

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Samuel Whiteford

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Liverpool |

characteristic: geographic location (region and locality)

text: Urban

characteristic: habitat

text: Samual Whiteford

characteristic: identified by

text: University ofLiverpool

text: larva

characteristic: lifestage

text: Luffia ferchaultella

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Luffia ferchaultella

text: FEMALE

characteristic: sex

text: NOT COLLECTED

characteristic: specimen voucher

text: 43b315a8-bd2f-49b7-855e-a7cc76347cb0-DTOL-specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Samuel Whiteford

text: University of Liverpool

text: United Kingdom

text: England | Liverpool |

text: Urban

text: Samual Whiteford

text: University ofLiverpool

text: larva

text: Luffia ferchaultella

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

text: Luffia ferchaultella

text: FEMALE

characteristic: specimen id

text: NOT COLLECTED

text: 82dfbb02-a53a-4c4c-b2e5-c239997862f5-DTOL-specimen

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Samuel Whiteford

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Liverpool |

characteristic: geographic location (region and locality)

text: Urban

characteristic: habitat

text: Samual Whiteford

characteristic: identified by

text: University ofLiverpool

text: larva

characteristic: lifestage

text: Luffia ferchaultella

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Luffia ferchaultella

text: FEMALE

text: NOT COLLECTED

text: ecdd9be4-848b-4cc0-892c-80924cf592f2-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Georgina Palmer

text: University of York

text: mountain ringlet

characteristic: common name

text: United Kingdom

text: England | Irton Fell

text: mountain grassland

text: Ilik Saccheri

text: University ofLiverpool

text: adult

text: Erebia epiphron

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: sample same as

text: Erebia epiphron

text: FEMALE

characteristic: sex

text: NOT COLLECTED

characteristic: specimen voucher

text: 014c7953-a8db-4442-8419-b44ce5e9eab6-dtol


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Georgina Palmer

characteristic: collected by

text: University of York

characteristic: collecting institution

characteristic: collection date

text: mountain ringlet

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Irton Fell

characteristic: geographic location (region and locality)

text: mountain grassland

characteristic: habitat

text: Ilik Saccheri

characteristic: identified by

text: University ofLiverpool

text: adult

characteristic: lifestage

text: Erebia epiphron

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Erebia epiphron

text: FEMALE

characteristic: specimen id

text: NOT COLLECTED

text: bb2b5c13-1d7e-4f93-8207-74f4302a3dc3-dtol

characteristic: title

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Samuel Whiteford

text: University of Liverpool

text: United Kingdom

text: England | Liverpool |

text: Urban

text: Samual Whiteford

text: University ofLiverpool

text: larva

text: Luffia ferchaultella

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: sample same as

text: Luffia ferchaultella

text: FEMALE

characteristic: sex

text: NOT COLLECTED

characteristic: specimen voucher

text: 6799f0ea-7002-4f4f-b598-23b8f52b0ea4-dtol


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Samuel Whiteford

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Liverpool |

characteristic: geographic location (region and locality)

text: Urban

characteristic: habitat

text: Samual Whiteford

characteristic: identified by

text: University ofLiverpool

text: larva

characteristic: lifestage

text: Luffia ferchaultella

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Luffia ferchaultella

text: FEMALE

characteristic: specimen id

text: NOT COLLECTED

text: 541acab1-f9f0-4a0a-9854-e7cd87dfaac2-dtol

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COPO

text: Samuel Whiteford

text: University of Liverpool

text: United Kingdom

text: England | Liverpool |

text: Urban

text: Samual Whiteford

text: University ofLiverpool

text: larva

text: Luffia ferchaultella

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: sample same as

text: Luffia ferchaultella

text: FEMALE

characteristic: sex

text: NOT COLLECTED

text: 377bec35-cd5b-4f0a-b262-528937a1cf83-dtol

characteristic: tolid


text: Sanger Institute

text: EarlhamInstitute

text: public

text: COPO

characteristic: broker name

text: Samuel Whiteford

characteristic: collected by

text: University of Liverpool

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: England | Liverpool |

characteristic: geographic location (region and locality)

text: Urban

characteristic: habitat

text: Samual Whiteford

characteristic: identified by

text: University ofLiverpool

text: larva

characteristic: lifestage

text: Luffia ferchaultella

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

characteristic: project name

text: Luffia ferchaultella

text: FEMALE

characteristic: specimen id

text: NOT COLLECTED

characteristic: specimen voucher

text: f0ad8e0b-d819-4b02-b452-2ce8ab39dc01-dtol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

text: Lighthouse Lab in Milton Keynes

text: Samples prepared at Lighthouse Lab in Milton Keynes by The Lighthouse Lab in Milton Keynes and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: Northern Ireland

text: Human

characteristic: host scientific name

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: title


text: SC

text: public

text: SC

characteristic: broker name

text: Lighthouse Lab in Glasgow

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: Human

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Lab in Alderley Park

text: Samples prepared at Lighthouse Lab in Alderley Park by Jacquelyn Wynn, Mairead Hyland, The Lighthouse Lab in Alderley Park and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: Northern Ireland

text: Human


text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SC

text: Lighthouse Lab in Milton Keynes

text: United Kingdom

text: England

text: Human


text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SC

text: Lighthouse Laboratory Plymouth

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: England

text: Human

characteristic: host scientific name


text: SC

text: public

text: SC

characteristic: broker name

text: Lighthouse Laboratory Plymouth

characteristic: collecting institution

text: United Kingdom

text: Scotland

text: Human

characteristic: sample capture status

characteristic: title


text: SC

text: public

text: SC

text: Lighthouse Lab in Alderley Park

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: Human

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: United Kingdom

text: England

text: Human


text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

text: England

text: Human


text: CHRISTIAN MEDICAL COLLEGE

text: public

characteristic: SRA accession

tag: Namespace:CHRISTIAN MEDICAL COLLEGE

characteristic: Submitter Id

text: India

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Salmonella enterica subsp. enterica serovar Typhi

text: Genomic surveillance of Salmonella Typhi


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: MANUELA KIENINGER

characteristic: collected by

text: SANGER INSTITUTE

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: EAST ANGLIA | ESSEX | SAFFRON WALDEN | WELLCOME TRUST SANGER INSTITUTE

text: AGAR PLATE IN LAB CULTURE

characteristic: habitat

text: PENNSYLVANIA STATE UNIVERSITY (TRENCH COLLECTION) USA

text: Heterorhabditis bacteriophora

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Heterorhabditis bacteriophora

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: symbiont

characteristic: title

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: MANUELA KIENINGER

text: SANGER INSTITUTE

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (region and locality)

text: AGAR PLATE IN LAB CULTURE

text: Heterorhabditis bacteriophora

characteristic: organism

text: WHOLE ORGANISM

characteristic: sample same as

text: Heterorhabditis bacteriophora

text: NOT PROVIDED

text: N


text: STANFORD UNIVERSITY

text: public

tag: Namespace:STANFORD UNIVERSITY

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Spinal cords were micro-dissected (Leica stereomicroscope) from E12.5 mice carrying a single Hb9::gfp+ allele and were dissociated with papain (papain dissociation kit, Worthington Biochemical) for 30 minutes. Tail clips were simultaneously taken from each embryo for genotyping. Spinal tissue was triturated and centrifuged at 1000rpm for 5 minutes. Dissociated cells were resuspended in 1:1 Neurobasal:DMEM/F12 (without phenol red) with 3% Horse Serum (Invitrogen) and DNase (Worthingon Biochemical) and were passed through a 35µm cell strainer (08-771-23, BD Falcon). Cells were sorted on a Becton Dickinson FACS Vantage SE DiVa using Coherent Sapphire 488nm solid state lasers (200mW). mRNA sequencing libraries were prepared using the TruSeq RNA Library Preparation Kit (v2) according to the manufacturer’s instructions (Illumina). Briefly, RNA with polyA+ tails was selected using oligo-dT beads. mRNA was then fragmented and reverse-transcribed into cDNA. cDNA was end-repaired, index adapter-ligated and PCR amplified. AMPure XP beads (Beckman Coulter) were used to purify nucleic acids after each step. Stranded mRNA-Seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit according to the manufacturer’s instructions (Illumina). Briefly, poly-A RNA was isolated using magnetic beads conjugated to poly-T oligos. mRNA was then fragmented and reverse-transcribed into cDNA. dUTPs were incorporated, followed by second strand cDNA synthesis. dUTP-incorporated second strand was not amplified. cDNA was then end-repaired, index adapter-ligated and PCR amplified. AMPure XP beads (Beckman Coulter) were used to purify nucleic acid after each steps of the library prep.

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

characteristic: genotype

text: 1 (litter 1)

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: mixed

characteristic: strain

text: WTa


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 6 (litter 2)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

text: mixed

characteristic: title


text: STANFORD UNIVERSITY

text: public

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

text: Hb9::gfp;miR-218-1KO/KO;miR-218-2KO/deltaILO

text: 10 (litter 5)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

text: mixed


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: genotype

text: 11 (litter 7)

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: female

characteristic: sex

text: mixed

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 12 (litter 8)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: male

text: mixed


text: STANFORD UNIVERSITY

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

text: 13 (litter 3)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: male

text: mixed

text: DKOa

characteristic: title


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: developmental stage

characteristic: genotype

text: 14 (litter 3)

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: female

text: mixed

text: DKOb


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 15 (litter 4)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

characteristic: sex

text: mixed

characteristic: strain

text: DKOc


text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: mouse embryo stage

text: 16 (litter 9)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

text: mixed


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

characteristic: genotype

text: 7 (litter 5)

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

text: mixed

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 8 (litter 5)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: female

text: mixed


text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 9 (litter 6)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: male

characteristic: sex

text: mixed

characteristic: strain


E-MTAB-10571:scRNA DKO_A_1

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E-MTAB-10571:scRNA DKO_A_1

text: E12 (in vivo)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Spinal cords were micro-dissected (Leica stereomicroscope) from E12.5 mice carrying a single Hb9::gfp+ allele and were dissociated with papain (papain dissociation kit, Worthington Biochemical) for 30 minutes. Tail clips were simultaneously taken from each embryo for genotyping. Spinal tissue was triturated and centrifuged at 1000rpm for 5 minutes. Dissociated cells were resuspended in 1:1 Neurobasal:DMEM/F12 (without phenol red) with 3% Horse Serum (Invitrogen) and DNase (Worthingon Biochemical) and were passed through a 35µm cell strainer (08-771-23, BD Falcon). Cells were sorted on a Becton Dickinson FACS Vantage SE DiVa using Coherent Sapphire 488nm solid state lasers (200mW). Sorted cells were spun down at 300 rcf for 2 minutes, and 370uL were removed to leave remaining 130 uL. The cells were resuspended in this remaining media. 32.5uL of the resuspended cells were loaded into 10X Chromium Controller using Chromium Single Cell 3’ v2 reagents. Sequencing library was prepared following the manufacturer’s instructions (10X Genomics).

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA DKO_A_1


E-MTAB-10571:scRNA DKO_A_2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10571:scRNA DKO_A_2

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA DKO_A_2

characteristic: title


E-MTAB-10571:scRNA DKO_A_3

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10571:scRNA DKO_A_3

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: single cell RNAseq

characteristic: protocol

text: Mus musculus

text: mixed

text: scRNA DKO_A_3


E-MTAB-10571:scRNA DKO_A_4

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E-MTAB-10571:scRNA DKO_A_4

text: E12 (in vivo)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

characteristic: strain

text: scRNA DKO_A_4


E-MTAB-10571:scRNA DKO_B_1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

text: E-MTAB-10571:scRNA DKO_B_1

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA DKO_B_1


E-MTAB-10571:scRNA DKO_B_2

text: STANFORD UNIVERSITY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10571:scRNA DKO_B_2

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA DKO_B_2

characteristic: title


E-MTAB-10571:scRNA DKO_B_3

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

text: E-MTAB-10571:scRNA DKO_B_3

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: mouse embryo stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: single cell RNAseq

characteristic: protocol

text: Mus musculus

text: mixed

text: scRNA DKO_B_3


E-MTAB-10571:scRNA DKO_B_4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

text: public

text: E-MTAB-10571:scRNA DKO_B_4

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

characteristic: strain

text: scRNA DKO_B_4


E-MTAB-10571:scRNA WT_A_1

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10571:scRNA WT_A_1

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA WT_A_1


E-MTAB-10571:scRNA WT_A_2

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10571:scRNA WT_A_2

characteristic: Submitter Id

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA WT_A_2

characteristic: title


E-MTAB-10571:scRNA WT_A_3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

text: public

text: E-MTAB-10571:scRNA WT_A_3

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: mouse embryo stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: single cell RNAseq

characteristic: protocol

text: Mus musculus

text: mixed

text: scRNA WT_A_3


E-MTAB-10571:scRNA WT_A_4

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

text: E-MTAB-10571:scRNA WT_A_4

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

characteristic: strain

text: scRNA WT_A_4


E-MTAB-10571:scRNA WT_B_1

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10571:scRNA WT_B_1

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA WT_B_1


E-MTAB-10571:scRNA WT_B_3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-10571:scRNA WT_B_3

characteristic: Submitter Id

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

text: scRNA WT_B_3

characteristic: title


E-MTAB-10571:scRNA WT_B_2

characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

text: E-MTAB-10571:scRNA WT_B_2

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

text: mouse embryo stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: single cell RNAseq

characteristic: protocol

text: Mus musculus

text: mixed

text: scRNA WT_B_2


E-MTAB-10571:scRNA WT_B_4

characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

text: public

text: E-MTAB-10571:scRNA WT_B_4

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: single cell RNAseq

text: Mus musculus

text: mixed

characteristic: strain

text: scRNA WT_B_4


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: day 6 (in vitro)

text: ArrayExpress

text: house mouse

text: Protocols: Hb9::gfp mES line (Lee et al., 2004) was cultured on MEF feeders using standard embryonic stem cell culture techniques. To generate spinal cord neurospheres, mESCs were dissociated with trypsin and 10^6 cells were plated in suspension in 10cm low-attachment dishes in ADFNK media [ADFNK media [Advanced D-MEM/F-12 (Life Technologies): Neurobasal medium (Life Technologies) (1:1), 10% Knockout Serum Replacement (Life Technologies), 200 mM L-Glutamine (Life Technologies), and 0.1 mM b-mercaptoethanol (Sigma)]. After two days, generated embryoid bodies were split onto three new 10-cm plates with fresh ADFNK media supplemented with 1 μM all-trans retinoic acid (RA, Sigma) and 100 nM smoothened agonist (SAG, Calbiochem). Two days later, freshly supplemented media was exchanged (Wichterle and Peljto, 2008). On day 6, neurospheres containing Hb9::gfp+ motor neurons were dissociated with papain (Worthington Biochemical), passed through a 40μm filter, and the resulting single cell suspension was used for fluorescence activated cell sorting. Cells were sorted on a Becton Dickinson FACS Vantage SE DiVa using Coherent Sapphire 488nm solid state lasers (200mW) and collected directly into Trizol LS. RNA was isolated according to Trizol LS protocol. mESCs were transfected with Lipofectamine 2000 with two pX330 vectors (containing Cas9 and two individual guide RNAs) and a plasmid expressing a puromycin resistance cassette. Guide RNAs were chosen to create specific multiplexed deletions between the selected gRNAs. 18 hours after transfection, mESCs were treated with puromycin for 48 hours to select for transfected cells. mESCs were split onto 10cm dishes with low density to allow for single colony selection. Colonies were manually picked after 5-8 days, manually dissociated, and split onto a feeder free gelatin coated plate for genotyping and MEF feeders for continued culture and freezing. Colonies were screened by PCR for wild type and knockout mutations to identify homozygous deletions of the indicated genomic regions. Selected mES lines that passed two rounds of PCR screening were expanded. mRNA sequencing libraries were prepared using the TruSeq RNA Library Preparation Kit (v2) according to the manufacturer’s instructions (Illumina). Briefly, RNA with polyA+ tails was selected using oligo-dT beads. mRNA was then fragmented and reverse-transcribed into cDNA. cDNA was end-repaired, index adapter-ligated and PCR amplified. AMPure XP beads (Beckman Coulter) were used to purify nucleic acids after each step. Stranded mRNA-Seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit according to the manufacturer’s instructions (Illumina). Briefly, poly-A RNA was isolated using magnetic beads conjugated to poly-T oligos. mRNA was then fragmented and reverse-transcribed into cDNA. dUTPs were incorporated, followed by second strand cDNA synthesis. dUTP-incorporated second strand was not amplified. cDNA was then end-repaired, index adapter-ligated and PCR amplified. AMPure XP beads (Beckman Coulter) were used to purify nucleic acid after each steps of the library prep.

text: differentiation day

text: Hb9:gfp deletion A

characteristic: genotype

text: ES-derived motor neuron differentiation

characteristic: growth condition

text: A

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

text: ESMNdelA


characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: day 6 (in vitro)

characteristic: age

text: ArrayExpress

text: house mouse

text: differentiation day

text: Hb9:gfp deletion B

text: ES-derived motor neuron differentiation

text: A

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

text: ESMNdelB

characteristic: title


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

text: public

text: day 6 (in vitro)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: differentiation day

text: Hb9:gfp deletion C

text: ES-derived motor neuron differentiation

text: A

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: mixed

text: ESMNdelC


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: day 6 (in vitro)

text: ArrayExpress

text: house mouse

text: differentiation day

characteristic: developmental stage

text: Hb9:gfp ctrl

characteristic: genotype

text: ES-derived motor neuron differentiation

text: A

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

characteristic: strain

text: ESMNWT


characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: day 6 (in vitro)

characteristic: age

text: ArrayExpress

text: house mouse

text: differentiation day

text: Hb9:gfp deletion D

text: ES-derived motor neuron differentiation

characteristic: growth condition

text: A

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

text: ESMNdelD


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

text: public

text: E12 (in vivo)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

text: 2 (litter 1)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: male

characteristic: sex

text: mixed

text: WTb

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E12 (in vivo)

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: developmental stage

characteristic: genotype

text: 3 (litter 2)

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: male

text: mixed

text: WTc


characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse embryo stage

text: 4 (litter 3)

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: female

text: mixed

characteristic: strain


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

text: public

text: E12 (in vivo)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

text: 5 (litter 4)

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: male

characteristic: sex

text: mixed


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: day 6 (in vitro)

text: ArrayExpress

text: house mouse

text: differentiation day

characteristic: developmental stage

text: Hb9:gfp deletion F

characteristic: genotype

text: ES-derived motor neuron differentiation

characteristic: growth condition

text: A

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

text: ESMNdelF

characteristic: title


characteristic: External Id

text: STANFORD UNIVERSITY

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: E12 (in vivo)

characteristic: age

text: ArrayExpress

text: house mouse

text: mouse embryo stage

characteristic: individual

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

characteristic: organism part

text: bulk RNAseq

characteristic: protocol

text: Mus musculus

text: mixed


characteristic: ENA-CHECKLIST

text: STANFORD UNIVERSITY

characteristic: INSDC first public

text: public

text: E12 (in vivo)

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: mouse embryo stage

text: WT

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: STANFORD UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: day 6 (in vitro)

text: ArrayExpress

text: house mouse

text: differentiation day

characteristic: developmental stage

text: Hb9:gfp deletion E

characteristic: genotype

text: ES-derived motor neuron differentiation

characteristic: growth condition

text: A

text: Mus musculus

text: Mus musculus

text: sorted Hb9:gfp+ motor neurons

text: bulk RNAseq

text: Mus musculus

text: mixed

text: ESMNdelE


characteristic: External Id

text: INRA-META

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: stool

characteristic: host body product

text: human-gut

characteristic: host body site

text: human gut metagenome

text: human gut metagenome

text: human gut metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

tag: Namespace:Respiratory Virus Unit, Microbiology Services Colindale, Public Health England

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Virology Department, Sheffield Teaching Hospitals NHS Foundation Trust/Department of Infection, Immunity and Cardiovascular Disease, The Medical School, University of Sheffield

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


text: Liverpool Clinical Laboratories

text: public

text: COVID-19 Genomics UK Consortium

text: Liverpool Clinical Laboratories

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Quadram Institute Bioscience

text: COVID-19 Genomics UK Consortium

text: Quadram Institute Bioscience

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: receipt date

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


text: public

tag: Namespace:Department of Pathology, University of Cambridge

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Centre for Enzyme Innovation, University of Portsmouth / Translational Research Laboratory, Portsmouth Hospitals NHS Trust / Hampshire Hospitals NHS Foundation Trust / Bournemouth University / University Hospitals Dorset NHS Foundation Trust / Brighton and Sussex University Hospitals NHS Trust / Dartford and Gravesham NHS Trust / Isle of Wight NHS Trust / Maidstone and Tunbridge Wells NHS Trust / University Hospital Southampton NHS Foundation Trust

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided