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characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3847-2207/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: LU

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3848-2208/2021


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: MCL Medizinische Laboratorien, Hauptstandort Niederwangen; Niederwangen, Switzerland

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-IFIK-3849-2691/2021

characteristic: isolate

characteristic: organism

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characteristic: title


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characteristic: ENA-LAST-UPDATE

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3850-2723/2021


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

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text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3851-2724/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-IFIK-3852-2725/2021

characteristic: isolate

characteristic: organism

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characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3853-2726/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3854-2727/2021

text: Severe acute respiratory syndrome coronavirus 2


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

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text: public

text: SIB

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

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characteristic: INSDC status

characteristic: SRA accession

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text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3856-2729/2021


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3857-2730/2021

text: active surveillance in response to outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: Luzerner Kantonsspital Luzern, Zentrum für Labormedizin ZLM; Luzern, Switzerland

text: Stefan Neuenschwander, Christian Baumann, Miguel A Terrazos Miani, Sonja Gempeler, Loïc Borcard, Nicole Liechti, Cora Sägesser, Pascal Bittel, Peter Keller, Franziska Suter-Riniker, Stephen L Leib, Alban Ramette

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/LU-IFIK-3858-2731/2021

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

text: Institute for Infectious Diseases, Bern, Switzerland

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3859-2732/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: LU

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3860-2733/2021


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/LU-IFIK-3861-2734/2021

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3862-2735/2021


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: LU

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/LU-IFIK-3863-2736/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: LU

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/LU-IFIK-3864-2737/2021

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: MCL Medizinische Laboratorien, Hauptstandort Niederwangen; Niederwangen, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-IFIK-3865-2738/2021


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Dr. Risch

characteristic: INSDC last update

text: public

tag: Namespace:Dr. Risch

text: SIB

text: Ender Diagnostics AG, Freiburgstrasse 251, 3018 Bern

text: Nadia Wohlwend, Faina Wehrli, Dominique Fabien Hilti, Sinem Kas, Martin Risch, Thomas Bodmer, Lorenz Risch

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/null-Risch-2192706183/2021

text: Severe acute respiratory syndrome coronavirus 2


characteristic: External Id

text: LUND UNIVERSITY

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-17-11-2021-02:13:37:278-6592

tag: Namespace:LUND UNIVERSITY

text: nontypeable Haemophilus influenzae isolate KR271

characteristic: description

text: Sweden

text: diseased

text: Homo sapiens

characteristic: isolate

text: blood

characteristic: isolation source

text: Haemophilus influenzae

characteristic: organism

text: Haemophilus influenzae

characteristic: title


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Health Services Laboratories

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Health Services Laboratories by Health Services Laboratories and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: Human

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Alderley Park

text: Samples prepared at Lighthouse Lab in Alderley Park by Jacquelyn Wynn, Mairead Hyland, The Lighthouse Lab in Alderley Park and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: Samples prepared at Lighthouse Lab in Milton Keynes by The Lighthouse Lab in Milton Keynes and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: England

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

text: Wales

text: Human

characteristic: title


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Laboratory Plymouth

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC

text: Integrated Covid Hub North East

text: Samples prepared at Integrated Covid Hub North East by Integrated Covid Hub North East and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

characteristic: description

text: United Kingdom

text: Scotland

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Lab in Glasgow

text: United Kingdom

text: England

text: Human

characteristic: title


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Alderley Park

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

text: Scotland

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: United Kingdom

text: Wales

characteristic: geographic location (region and locality)

text: Human

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Samples prepared at Virology Department, Royal Infirmary of Edinburgh, NHS Lothian / School of Biological Sciences, University of Edinburgh by McHugh M, Dewar R, Rooke S, O'Toole Á, Scher E, Hill V, McCrone JT, Colquhoun R, Yu X, Jackson B, Rambaut A, Templeton K and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Samples prepared at Berkshire and Surrey Pathology Services Lighthouse Laboratory by Berkshire and Surrey Pathology Services Lighthouse Laboratory and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

characteristic: description

text: United Kingdom

text: England

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Virology Department, Royal Infirmary of Edinburgh, NHS Lothian / School of Biological Sciences, University of Edinburgh

text: United Kingdom

text: Scotland

text: Human

characteristic: title


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Laboratory Plymouth

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

text: Wales

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC

text: Integrated Covid Hub North East

text: United Kingdom

text: Wales

characteristic: geographic location (region and locality)

text: Human

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Berkshire and Surrey Pathology Services Lighthouse Laboratory

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Health Services Laboratories

characteristic: description

text: United Kingdom

text: England

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: United Kingdom

text: Wales

text: Human

characteristic: title


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Milton Keynes

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

text: England

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

characteristic: description

text: United Kingdom

text: Scotland

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Lab in Milton Keynes

text: United Kingdom

text: Wales

text: Human

characteristic: title


text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Glasgow

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

text: Scotland

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Laboratory Plymouth

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: Human

characteristic: host scientific name


characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Rosalind Franklin Laboratory

text: Samples prepared at Rosalind Franklin Laboratory by Donald Fraser, Suki Lee, Rob Howes, The Rosalind Franklin Laboratory and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

characteristic: description

text: United Kingdom

text: England

text: Human

characteristic: organism


text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

text: Scotland

text: Human

characteristic: title


text: public

tag: Namespace:Laboratory of Genomics and Bioinformatics, Comenius University Science Park

characteristic: Submitter Id

text: Science Park of Comenius University of Bratislava

characteristic: broker name

text: Public Health Authority of the Slovak Republic, Trnavská cesta 52, 826 45 Bratislava, Slovakia

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: common name

text: Slovakia

unit: year

characteristic: host age

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

text: female

characteristic: host sex

characteristic: host subject id

text: RNA

characteristic: isolate

text: Nasopharyngeal swab

characteristic: isolation source-host associated


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratory of Genomics and Bioinformatics, Comenius University Science Park

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: not provided

text: Slovakia

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: male

text: RNA

text: Nasopharyngeal swab

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: sample capture status


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: common name

text: Slovakia

characteristic: host age

text: Human

characteristic: host common name

text: not provided

text: Homo sapiens

text: female

text: RNA

text: Nasopharyngeal swab


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: APC Microbiome Institute

characteristic: INSDC center name

text: public

tag: Namespace:APC Microbiome Institute

characteristic: description

text: human gut metagenome

text: human gut metagenome

characteristic: title


text: Istituto Italiano di Tecnologia

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Istituto Italiano di Tecnologia

text: human

text: CaCo2 cells infected with SARS-CoV-2

text: Homo sapiens

characteristic: organism

text: Homo sapiens


text: EMBL

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: doxycycline

characteristic: compound

text: Protocols: TX1072 are XX mouse embryonic cell line mixed Cast/BL6 (Shultz et al., 2014) that carry a DOX inducible Xist on the Bl6 allele. To induce XCI culture ES cells with 100 ug/ml of DOX for 6 days in DMEM (Sigma), 15% FBS (Gibco), 1% Penicillin streptavidin, 0.1 mM _-mercaptoethanol, 1000 U/ml (LIF), CHIR99021 (3 _M), PD0325901 (1 _M). To induce XCR wash out DOX (D-wo) and harvest samples for analysis at the following time-series: 1d D-wo, 3d D-wo and 7d D-wo. total RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB) (#T2010S) Libraries for RNA-seq by enrichment of PolyA+ mRNA were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)

unit: microgram per milliliter

characteristic: dose

text: gene knock out

characteristic: genetic modification

text: XX; Dppa2 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

unit: day

characteristic: time

text: 1 day DOX washout

characteristic: treatment


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL

characteristic: INSDC center name

characteristic: INSDC first public

text: public

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text: 1 day DOX washout


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text: mouse embryonic stem cell

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text: mouse embryonic stem cell

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text: Esg1-tdTomato reporter mESC line

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text: Protocols: Esg1-tdTomato or p53-tdTomato reporter ESC line containing inducible dCas9::KRAB or dCas9::GFP control and target-specific gRNA have bee treated with DOX (100 ng/ml) for 7 days to impose epigenetic silencing of the reporter. Memory of the silenced state has been assayed after 4 or 7 days of DOX washout. This protocol has been optimised from Skene and Henikoff, Elife, 2017 to detect interaction between DNA and histone modifications. Briefly, fresh cells are harvested, permeabilised, bound to concanavallin magnetic beads and incubated with the antibody for the modification of interest. Samples were then incubated with pA-MNase to cut the DNA side of the bound antibody and the DNA fragments were eluted and subjected to size selection. Finally DNA library was generated with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S).

characteristic: description

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text: Hopital du Jura: Delemont, Delemont, Switzerland

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text: Tim Roloff, Fanny Wegner, Helena MB Seth-Smith, Alfredo Mari, Karoline Leuzinger, Julia Bielicki, Manuel Battegay, Hans Hirsch, Adrian Egli

characteristic: collector name

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characteristic: ENA-CHECKLIST

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