E-MTAB-111261637921679:Catalase PLK4_1

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-111261637921679:Catalase PLK4_1

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: PLK4 transient overexpression

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Catalase PLK4_1


E-MTAB-111261637921679:Catalase PLK4_2

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:Catalase PLK4_2

tag: Namespace:DANA-FARBER CANCER INSTITUTE

text: ArrayExpress

characteristic: broker name

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cells are scraped from 6 well plates. All source cell culture was maintained in media containing plasmocin (1:5000 dilution, Invivogen) to prevent mycoplasma contamination. Culture plates were briefly coated with 40μg/ml of fibronectin (Sigma) and glass coverslips were incubated in 40 μg/ml fibronectin for at least 2 hours. Human mammary epithelial MCF10A cells were maintained at 370C with 5% CO2 atmosphere and cultured as previously described18. In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Telomerase-immortalized RPE-1 cells (ATCC), and all derivative cell lines generated in this study were cultured in phenol red-free DMEM/F12 media containing 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin. At day 0, control 608 and PLK4 cells were gently dissociated into single cells, then seeded at low density into media with 2μg/ml of doxycycline such that they were ~80% confluent on day 5. After ~48 hours of doxycycline incubation, the culture media with doxycycline was replaced. On day 5, cells are gently dissociated into single cells before reseeding into 6 well plates to ~80% confluent on day 7. Because control 608 cells grow faster than PLK4 induced cells, control 608 cells are seeded at ~2.5 times lower density than PLK4 induced cells. Cells at ~80% confluency are analysed either at day 7 or day 8. Media is left unchanged for ~3 days before analysis. RPE-1 cells grown in 5% oxygen chamber To conditionally overexpress PLK4, lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and pLenti-CMV/TO-Neo-Dest (17292, Addgene) were used. Wild-type PLK4 and PLK4 1-608 cDNAs (control 608) were cloned using the Gateway system into the pLenti-CMV/TO-Neo-Dest vector Cells were coinfected with a lentivirus containing the TetR and with the lentivirus containing the wild-type PLK4 or control 608 open reading frames. Transduced cells were selected with Blasticidin (10 μg/ml) and Geneticin (200μg/ml). control 608 and PLK4 overexpression was induced transiently. Transduced cells that survive the selection are used for subsequent experiments and a maintenance dose of 5 μg/ml of Blasticidin and 80μg/ml of Geneticin was used in stock cultures. MCF10A cells were exposed to 12Gy gamma irradiation RNeasy kit was used to extract the RNA from the cell lysates NextSeq500 RNA Library Preparation and Sequencing RNA quantity was determined on the Qubit using the Qubit RNA Assay Kit (Life Tech) and RNA quality was determined on the Bioanalyzer using the RNA Pico Kit (Agilent). Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A) mRNA, 100ng of total RNA was used for cDNA library construction, according to the 'manufacturer's protocol.

characteristic: description

text: normal

characteristic: disease

text: PLK4 transient overexpression

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Catalase PLK4_2

characteristic: title


E-MTAB-111261637921679:Catalase PLK4_3

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-111261637921679:Catalase PLK4_3

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: PLK4 transient overexpression

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Catalase PLK4_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:Control Gamma-irradiation1

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Control Gamma-irradiation1


text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:Control Gamma-irradiation2

text: ArrayExpress

characteristic: broker name

text: MCF 10A

characteristic: cell line

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Control Gamma-irradiation2


text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:Control Gamma-irradiation3

text: ArrayExpress

text: MCF 10A

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Control Gamma-irradiation3


text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:Experimental Gamma-irradiation4

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Gamma irradiation

characteristic: stimulus

text: Experimental Gamma-irradiation4

characteristic: title


characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-111261637921679:Experimental Gamma-irradiation5

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Gamma irradiation

text: Experimental Gamma-irradiation5


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:Experimental Gamma-irradiation6

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Gamma irradiation

text: Experimental Gamma-irradiation6


E-MTAB-111261637921679:HIF1A KO1 608

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:HIF1A KO1 608

text: ArrayExpress

characteristic: broker name

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

text: PLK4-608 mutant transient overexpression; HIF1A knockout

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: HIF1A KO1 608


E-MTAB-111261637921679:HIF1A KO2 608

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:HIF1A KO2 608

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

characteristic: disease

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: HIF1A KO2 608


E-MTAB-111261637921679:HIF1A KO3 608

characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-111261637921679:HIF1A KO3 608

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: HIF1A KO3 608

characteristic: title


E-MTAB-111261637921679:HIF1A KO4 PLK4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DANA-FARBER CANCER INSTITUTE

text: public

characteristic: SRA accession

text: E-MTAB-111261637921679:HIF1A KO4 PLK4

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: PLK4 transient overexpression; HIF1A knockout

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: HIF1A KO4 PLK4


E-MTAB-111261637921679:HIF1A KO5 PLK4

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:HIF1A KO5 PLK4

text: ArrayExpress

characteristic: broker name

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: HIF1A KO5 PLK4


E-MTAB-111261637921679:HIF1A KO6 PLK4

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:HIF1A KO6 PLK4

text: ArrayExpress

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: HIF1A KO6 PLK4


E-MTAB-111261637921679:RPE-1 608 1

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:RPE-1 608 1

text: ArrayExpress

text: epithelial cell

text: human

text: normal

characteristic: disease

text: PLK4-608 mutant transient overexpression

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: retina

characteristic: organism part

text: Homo sapiens

text: RPE-1 608 1


E-MTAB-111261637921679:RPE-1 608 2

characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-111261637921679:RPE-1 608 2

text: ArrayExpress

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: RPE-1 608 2

characteristic: title


E-MTAB-111261637921679:RPE-1 608 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DANA-FARBER CANCER INSTITUTE

text: public

characteristic: SRA accession

text: E-MTAB-111261637921679:RPE-1 608 3

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: RPE-1 608 3


E-MTAB-111261637921679:RPE-1 PLK4 4

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:RPE-1 PLK4 4

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: normal

text: PLK4 mutant transient overexpression

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: RPE-1 PLK4 4


E-MTAB-111261637921679:RPE-1 PLK4 5

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:RPE-1 PLK4 5

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

text: PLK4 mutant transient overexpression

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: RPE-1 PLK4 5


E-MTAB-111261637921679:RPE-1 PLK4 6

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:RPE-1 PLK4 6

text: ArrayExpress

text: epithelial cell

text: human

text: normal

characteristic: disease

text: PLK4 mutant transient overexpression

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: retina

characteristic: organism part

text: Homo sapiens

text: RPE-1 PLK4 6


characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-111261637921679:SW1_608_KO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: Protocols: Cells are scraped from 6 well plates. All source cell culture was maintained in media containing plasmocin (1:5000 dilution, Invivogen) to prevent mycoplasma contamination. Culture plates were briefly coated with 40μg/ml of fibronectin (Sigma) and glass coverslips were incubated in 40 μg/ml fibronectin for at least 2 hours. Human mammary epithelial MCF10A cells were maintained at 370C with 5% CO2 atmosphere and cultured as previously described18. In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Telomerase-immortalized RPE-1 cells (ATCC), and all derivative cell lines generated in this study were cultured in phenol red-free DMEM/F12 media containing 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin. At day 0, control 608 and PLK4 cells were gently dissociated into single cells, then seeded at low density into media with 2μg/ml of doxycycline such that they were ~80% confluent on day 5. After ~48 hours of doxycycline incubation, the culture media with doxycycline was replaced. On day 5, cells are gently dissociated into single cells before reseeding into 6 well plates to ~80% confluent on day 7. Because control 608 cells grow faster than PLK4 induced cells, control 608 cells are seeded at ~2.5 times lower density than PLK4 induced cells. Cells at ~80% confluency are analysed either at day 7 or day 8. Media is left unchanged for ~3 days before analysis. RPE-1 cells grown in 5% oxygen chamber To conditionally overexpress PLK4, lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and pLenti-CMV/TO-Neo-Dest (17292, Addgene) were used. Wild-type PLK4 and PLK4 1-608 cDNAs (control 608) were cloned using the Gateway system into the pLenti-CMV/TO-Neo-Dest vector Cells were coinfected with a lentivirus containing the TetR and with the lentivirus containing the wild-type PLK4 or control 608 open reading frames. Transduced cells were selected with Blasticidin (10 μg/ml) and Geneticin (200μg/ml). control 608 and PLK4 overexpression was induced transiently. Transduced cells that survive the selection are used for subsequent experiments and a maintenance dose of 5 μg/ml of Blasticidin and 80μg/ml of Geneticin was used in stock cultures. Lentivirus was used to deliver the CRISPR knockout Trio to MCF10A cells. Puromycin was used to select cells with Trio knockout. MCF10A cells were exposed to 12Gy gamma irradiation RNeasy kit was used to extract the RNA from the cell lysates NextSeq500 RNA Library Preparation and Sequencing RNA quantity was determined on the Qubit using the Qubit RNA Assay Kit (Life Tech) and RNA quality was determined on the Bioanalyzer using the RNA Pico Kit (Agilent). Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A) mRNA, 100ng of total RNA was used for cDNA library construction, according to the 'manufacturer's protocol.

text: normal

text: PLK4-608 mutant transient overexpression; Trio knockout

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DANA-FARBER CANCER INSTITUTE

text: public

characteristic: SRA accession

text: E-MTAB-111261637921679:SW1_608_R1_.fc1.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: PLK4 transient overexpression

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:SW2_608_KO_R1_.fc1.fastq.gz

text: ArrayExpress

characteristic: broker name

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:SW26_Control_DMSO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cells are scraped from 6 well plates. All source cell culture was maintained in media containing plasmocin (1:5000 dilution, Invivogen) to prevent mycoplasma contamination. Culture plates were briefly coated with 40μg/ml of fibronectin (Sigma) and glass coverslips were incubated in 40 μg/ml fibronectin for at least 2 hours. Human mammary epithelial MCF10A cells were maintained at 370C with 5% CO2 atmosphere and cultured as previously described18. In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Telomerase-immortalized RPE-1 cells (ATCC), and all derivative cell lines generated in this study were cultured in phenol red-free DMEM/F12 media containing 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin. At day 0, control 608 and PLK4 cells were gently dissociated into single cells, then seeded at low density into media with 2μg/ml of doxycycline such that they were ~80% confluent on day 5. After ~48 hours of doxycycline incubation, the culture media with doxycycline was replaced. On day 5, cells are gently dissociated into single cells before reseeding into 6 well plates to ~80% confluent on day 7. Because control 608 cells grow faster than PLK4 induced cells, control 608 cells are seeded at ~2.5 times lower density than PLK4 induced cells. Cells at ~80% confluency are analysed either at day 7 or day 8. Media is left unchanged for ~3 days before analysis. RPE-1 cells grown in 5% oxygen chamber To conditionally overexpress PLK4, lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and pLenti-CMV/TO-Neo-Dest (17292, Addgene) were used. Wild-type PLK4 and PLK4 1-608 cDNAs (control 608) were cloned using the Gateway system into the pLenti-CMV/TO-Neo-Dest vector Cells were coinfected with a lentivirus containing the TetR and with the lentivirus containing the wild-type PLK4 or control 608 open reading frames. Transduced cells were selected with Blasticidin (10 μg/ml) and Geneticin (200μg/ml). control 608 and PLK4 overexpression was induced transiently. Transduced cells that survive the selection are used for subsequent experiments and a maintenance dose of 5 μg/ml of Blasticidin and 80μg/ml of Geneticin was used in stock cultures. Overnight treatment of MCF10A with 1μm of DMOG, with DMSO treated cells as control. MCF10A cells were exposed to 12Gy gamma irradiation RNeasy kit was used to extract the RNA from the cell lysates NextSeq500 RNA Library Preparation and Sequencing RNA quantity was determined on the Qubit using the Qubit RNA Assay Kit (Life Tech) and RNA quality was determined on the Bioanalyzer using the RNA Pico Kit (Agilent). Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A) mRNA, 100ng of total RNA was used for cDNA library construction, according to the 'manufacturer's protocol.

characteristic: description

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DMSO control

characteristic: stimulus


characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:SW27_Control_DMSO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: DMSO control


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-111261637921679:SW28_Treated_DMOG_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DMOG treatment

characteristic: title


characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-111261637921679:SW29_Treated_DMOG_R1_.fc1.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: DMOG treatment


text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:SW3_608_KO_R1_.fc1.fastq.gz

text: ArrayExpress

characteristic: broker name

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:SW4_PLK4_KO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

characteristic: disease

text: PLK4 transient overexpression; Trio knockout

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

text: E-MTAB-111261637921679:SW5_PLK4_KO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-111261637921679:SW6_PLK4_KO_R1_.fc1.fastq.gz

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: GISAID Accession ID

characteristic: INSDC first public

text: public

characteristic: SRA accession

tag: Namespace:Institute for Infectious Diseases, Bern, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Spital Langnau i.E.; Langnau i.E., Switzerland

characteristic: collecting institution

characteristic: collection date

text: Nicole Liechti, Christian Baumann, Miguel A Terrazos Miani, Sonja Gempeler, Loïc Borcard, Stefan Neuenschwander, Cora Sägesser, Pascal Bittel, Peter Keller, Franziska Suter-Riniker, Stephen L Leib, Alban Ramette

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-IFIK-4291-9564/2021

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status

text: COVID-HUB-PL deep NGS sequencing of SARS-CoV-2 genomes


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: Slaskie

characteristic: geographic location (region and locality)

text: human

text: not provided

text: Homo sapiens

text: female

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Slaskie

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Male

characteristic: host sex

characteristic: host subject id


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: human

text: not provided

text: Homo sapiens

text: male

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Opolskie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

text: female

characteristic: organism

characteristic: title


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: Malopolskie

text: human

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Malopolskie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

text: male

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: Dolnoslaskie

text: human

text: not provided

characteristic: host health state

text: Homo sapiens

text: male

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: ?l?skie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: ?l?skie

text: human

text: not provided

text: Homo sapiens

text: male

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Wielkopolskie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

text: male

characteristic: organism

characteristic: title


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: Mazowieckie

text: human

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lubuskie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

text: female

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: WIelkopolskie

text: human

text: not provided

characteristic: host health state

text: Homo sapiens

text: female

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: Gyncentrum, Sosnowiec, Poland

text: Emilia Morawiec

characteristic: description

text: Poland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Opolskie

characteristic: geographic location (region and locality)

characteristic: host age

text: human

characteristic: host common name

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id


text: COVID-HUB-PL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Gyncentrum, Sosnowiec, Poland

characteristic: collecting institution

characteristic: collection date

text: Emilia Morawiec

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: Mazowieckie

text: human

text: not provided

text: Homo sapiens

text: male

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status


E-MTAB-11201:Input 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11201:Input 1

tag: Namespace:UCL Institute of Neurology The Francis Crick Institute

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Norway rat

characteristic: common name

text: Protocols: Rat cerebral cortex were dissected and snap frozen. Tissue was ground into a fine powder using mortar and pestle whilst being kept frozen with liquid nitrogen. Subsequently, the tissue dry powder was spread across 6-well plates, pre-chilled on dry ice, and UVC crosslinked at 150mJ/cm2 for 4 rounds with shaking in between to ensure even crosslinking on dry ice. Buffers used and procedures follow the iiCLIP (Lee et al. 2021, biorxiv) and hiCLIP protocols (Sugimoto et al. 2017). Briefly, the crosslinked tissue was lysed in CLIP lysis buffer with sonication to aid lysis. The RNA was fragmented in lysate (1.5U/mg RNase I, 3 minutes at 37°C). The lysate was then added to the protein A Dynabeads, which had been pre-incubated with rabbit polyclonal STAU2 antibody (generated by the Kiebler lab) to specifically capture the endogenous protein and the crosslinked RNAs, and incubated for 2 hours at 4°C. After eluting the IP, samples were run on SDS-PAGE and RNAs were phenol-chloroform extracted from the nitrocellulose membrane. Fragmented RNA is 3' end dephosphorylated and ligated to an infrared adapter. RNA is reverse transcribed using SuperScript IV, and circularised by circligase II. Libraries were amplified by PCR with Phusion HF.

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: cerebral cortex

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Input 1

characteristic: title


E-MTAB-11201:Input 2

text: UCL Institute of Neurology The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11201:Input 2

characteristic: Submitter Id

text: ArrayExpress

text: Norway rat

text: juvenile stage

text: normal

text: wild type genotype

text: Rattus norvegicus

text: Rattus norvegicus

text: cerebral cortex

text: Rattus norvegicus

text: male

text: Input 2


E-MTAB-11201:Input 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11201:Input 3

characteristic: age

text: ArrayExpress

text: Norway rat

text: juvenile stage

text: normal

text: wild type genotype

text: Rattus norvegicus

text: Rattus norvegicus

text: cerebral cortex

text: Rattus norvegicus

text: male

text: Input 3


E-MTAB-11201:Sample 1

text: public

text: E-MTAB-11201:Sample 1

text: ArrayExpress

characteristic: broker name

text: Norway rat

characteristic: common name

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Rattus norvegicus

text: Rattus norvegicus

text: cerebral cortex

text: Rattus norvegicus

text: male

text: Sample 1


E-MTAB-11201:Sample 2

text: public

text: E-MTAB-11201:Sample 2

text: ArrayExpress

text: Norway rat

text: juvenile stage

text: normal

text: wild type genotype

characteristic: genotype

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: cerebral cortex

characteristic: organism part

text: Rattus norvegicus

text: male

characteristic: sex

text: Sample 2

characteristic: title


E-MTAB-11201:Sample 3

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11201:Sample 3

text: ArrayExpress

text: Norway rat

text: juvenile stage

text: normal

text: wild type genotype

text: Rattus norvegicus

text: Rattus norvegicus

text: cerebral cortex

text: Rattus norvegicus

text: male

text: Sample 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ena-SAMPLE-TAB-26-11-2021-15:55:26:933-11137

tag: Namespace:VISAVET Health Surveillance Centre (UCM)

characteristic: Submitter Id

characteristic: collection date

text: SALMONELLA

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: isolate

text: Sewage water

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Enteritidis

text: Enteritidis

characteristic: serovar


text: VISAVET Health Surveillance Centre (UCM)

text: public

text: ena-SAMPLE-TAB-26-11-2021-15:55:26:934-11138

text: SALMONELLA

characteristic: description

text: Spain

text: Sewage water

characteristic: organism

text: Enteritidis


characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-26-11-2021-15:55:26:934-11142

text: SALMONELLA

text: Spain

text: Sewage water

text: Enteritidis

characteristic: title


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:University Hospital Basel

text: SIB

characteristic: broker name

text: Clinical Virology, University Hospital Basel, Basel, Switzerland

characteristic: collecting institution

text: Tim Roloff, Fanny Wegner, Helena MB Seth-Smith, Alfredo Mari, Karoline Leuzinger, Julia Bielicki, Manuel Battegay, Hans Hirsch, Adrian Egli

characteristic: collector name

text: Switzerland

text: BL

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BL-UHB-43035863/2021

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43040894/2021

characteristic: isolate


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43042486/2021

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43044480/2021

characteristic: sample capture status


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43138278/2021

characteristic: isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43139331/2021

characteristic: organism

characteristic: title


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43139435/2021

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43139479/2021

characteristic: isolate


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43139503/2021

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43139595/2021

characteristic: sample capture status


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43140164/2021

characteristic: isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43140169/2021

characteristic: organism

characteristic: title


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43141295/2021

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

text: Clinical Virology, University Hospital Basel, Basel, Switzerland

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43141675/2021

characteristic: isolate


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

text: Tim Roloff, Fanny Wegner, Helena MB Seth-Smith, Alfredo Mari, Karoline Leuzinger, Julia Bielicki, Manuel Battegay, Hans Hirsch, Adrian Egli

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43141856/2021

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43142246/2021

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: collection date

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43142457/2021

characteristic: isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Basel

text: public

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-UHB-43145309/2021

characteristic: organism

characteristic: title


characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BS

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-UHB-43145326/2021

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