text: CNRS - BPMP

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Columbia

text: mid rosette growth stage

text: clf-29 lhp1-4

text: low nitrate

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Max Planck Institute of Immunobiology and Epigenetics (MPI-IE)

unit: week

characteristic: age

text: ArrayExpress

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Six month-old C57BL6 mice were sacrificed and bone marrow (BM) cells were isolated from pooled femura, tibiae, hips, ilia, and vertebrae by gentle crushing in PBS using a mortar and pistil. Erythrocyte lysis was performed using ACK Lysing Buffer (Thermo Fisher Scientific). To enrich for lineage negative (Lin-) cells we used the Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Briefly, the ery-lysed BM was stained for 30-45 min with a 1:4 dilution of the Lineage Cocktail provided in the Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Labelled cells were then incubated for 20 min with 400uL/mouse of washed polyclonal sheep anti-rat IgG coated Dynabeads provided in the Kit. Depletion of Lin+ cells was performed using a magnet. To achieve further purification of hematopoietic stem and progenitor cells we used fluorescence- activated cell sorting (FACS). Therefore, the depleted cell fraction was stained for 30 min to 1h using the following monoclonal antibodies: anti-lineage [anti-CD4 (clone GK1.5), anti-CD8a (53-6.7), anti-CD11b (M1/70), anti- B220 (RA3-6B2), anti-GR1 (RB6-8C5) and anti-TER119 (Ter-119)] all PE-Cy7 or BV650 or FITC; anti-CD117/c-Kit (2B8)-PE or BV711; anti-Ly6a/Sca-1 (D7)-APCCy7; anti-CD34 (RAM34)-FITC or AF700; anti-CD150 (TC15-12F12.2)-BV605 or PECy5; anti-CD48 (HM48-1)-BV421 or PECy7. Monoclonal antibody conjugates were purchased from eBioscience or BioLegend. Sorting was then performed on a FACS Aria II, III or Symphony (Becton Dickinson) at the MPI FACS facility, Freiburg, Germany. The following sort parameters were used: 100 m nozzle; 10,000 evt/s.; 70 psi. Each 2000 LT-HSCs (Lin-negative, Sca1-positive, c-Kit-positive, CD150-positive, CD48-negative, CD34-negative) and 10.000 MPP3/4 (Lin-negative, Sca1-positive, c-Kit-positive, CD150-negative, CD48-positive) were sorted, respectively, in 3 biological replicates. Cells were sorted into PBS 1%FCS, spun down and resuspended in 20ul transposition buffer (1ul Tn5, 10ul 2x TD buffer (Illumina), 0.01% digitonin, 0.3x PBS). Transposase reaction was done for 30min at 37°C before DNA was isolated by Qiagen MinElute Clean up kit. Libraries were amplified at 14 cycles for LT-HSCs and 11 cycles for MPP3/4 and DNA was isolated using AMPure XP beads (Beckman Coultier).

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mouse 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Max Planck Institute of Immunobiology and Epigenetics (MPI-IE)

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

text: house mouse

text: adult

text: wild type genotype

text: Mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: fresh specimen

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: fresh specimen


text: public

text: ArrayExpress

text: hematopoietic multipotent progenitor cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mouse 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: fresh specimen

characteristic: specimen with known storage state

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: wild type genotype

text: Mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: fresh specimen

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: age

text: ArrayExpress

characteristic: cell type

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: fresh specimen


LMG 7974

text: LM-UGent

text: public

text: LMG 7974

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: LMG 7974

characteristic: isolate

text: Pig

characteristic: isolation source

text: Campylobacter sp. LMG 7974

text: Campylobacter sp. LMG 7974

text: LMG 7974

characteristic: strain

text: Campylobacter sp. LMG 7974


LMG 8286

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: LM-UGent

text: public

characteristic: SRA accession

text: LMG 8286

text: Germany

text: LMG 8286

text: Pig

text: Campylobacter sp. LMG 8286

text: Campylobacter sp. LMG 8286

text: LMG 8286

text: Campylobacter sp. LMG 8286


text: UNIL

text: public

characteristic: Submitter Id

text: Control condition, biological replicate #1. This control correspond to the Tn library of Pseudomonas protegens Pf-5, non-exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

characteristic: description

text: Pseudomonas protegens Pf-5

characteristic: organism

text: Pseudomonas protegens Pf-5

characteristic: title


characteristic: External Id

text: UNIL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Control condition, biological replicate #2. This control correspond to the Tn library of Pseudomonas protegens Pf-5, non-exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

text: Pseudomonas protegens Pf-5

text: Pseudomonas protegens Pf-5


text: UNIL

text: public

characteristic: INSDC status

text: Control condition, biological replicate #3. This control correspond to the Tn library of Pseudomonas protegens Pf-5, non-exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

text: Pseudomonas protegens Pf-5

text: Pseudomonas protegens Pf-5


text: UNIL

text: public

text: Biological replicate #1. The Pf-5 Tn library was exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

text: Pseudomonas protegens Pf-5

text: Pseudomonas protegens Pf-5


text: UNIL

text: public

text: Biological replicate #2. The Pf-5 Tn library was exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

text: Pseudomonas protegens Pf-5

text: Pseudomonas protegens Pf-5


text: UNIL

text: public

text: Biological replicate #3. The Pf-5 Tn library was exposed to the tailocin #1 produced by its kin Pseudomonas protegens CHA0.

text: Pseudomonas protegens Pf-5

text: Pseudomonas protegens Pf-5


text: UNIL

text: public

text: Biological replicate #1. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #1 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #2. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #1 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #3. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #1 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #1. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #2 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #2. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #2 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #3. The Tn library of Pseudomonas protegens CHA0 was exposed to its own tailocin #2 to identify genetic determinants involved in self-immunity.

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #1. This control corresponds to the Tn library of Pseudomonas protegens CHA0 that was not exposed to its own two tailocins (neither tailocin #1 nor tailocin #2).

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #2. This control corresponds to the Tn library of Pseudomonas protegens CHA0 that was not exposed to its own two tailocins (neither tailocin #1 nor tailocin #2).

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: UNIL

text: public

text: Biological replicate #3. This control corresponds to the Tn library of Pseudomonas protegens CHA0 that was not exposed to its own two tailocins (neither tailocin #1 nor tailocin #2).

text: Pseudomonas protegens CHA0

text: Pseudomonas protegens CHA0


text: CENTER FOR GENOMIC REGULATION

text: public

tag: Namespace:CENTER FOR GENOMIC REGULATION

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: precursor B cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cells were collected at twelve time points after transdifferentiation induction: 0, 3, 6, 9, 12, 18, 24, 36, 48, 72, 120 and 168 hours. Cell pellets were frozen prior to RNA extraction. Human BLaER1 cells were grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS, Gibco), 2 mM L-glutamine, and 100 U/ml penicillin G sodium (Gibco). Cells were maintained at 37ºC in a humid atmosphere containing 5% CO2. Transdifferentiation was induced by addition of 100 nM of beta-estradiol (Calbiochem) and 10 nM of IL-3 and CSF-1 (Peprotech). Cells were lysed with QiAzol (Qiagen, The Netherlands). Chloroform was added to each sample, and RNA contained in the aqueous solution was isolated and purified by using RNeasy mini kit columns (Qiagen, The Netherlands). Poly A+ libraries were prepared with 1 ug of total RNA and using TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) according to the manufacturer’s protocol.

text: chronic lymphocytic leukemia

characteristic: disease

text: C/EBPER-GFP

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: CENTER FOR GENOMIC REGULATION

text: public

characteristic: SRA accession

text: ArrayExpress

text: precursor B cell

text: human

text: chronic lymphocytic leukemia

text: C/EBPER-GFP

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: transdifferentiation to macrophage

characteristic: stimulus


E-MTAB-9808:Sample 14

characteristic: External Id

text: public

text: E-MTAB-9808:Sample 14

tag: Namespace:Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

text: Protocols: Peripheral blood mononuclear cells (PBMCs) were isolated 10 ml whole blood samples taken from anterior vena cava punctures and collected into pre-chilled tubes with EDTA. The blood samples were then centrifuged on a Histopaque-1077 density gradient (Sigma-Aldrich, Taufkirchen, Germany) following the manufacturer’s instructions to recover the layer of PBMCs. The isolated PBMCs were stored in liquid nitrogen with 90% fetal bovine serum (FBS; PAA, Cölbe, Germany) and 10% DMSO until use. PBMCs were thawed and re-suspended in cell culture medium (RPMI 1640 medium supplemented with 10% FBS, 2 mmol/l L-glutamine (Biochrom, Berlin, Germany), 100 U/ml penicillin (PAA, Cölbe, Germany), and 100 mg/ml streptomycin (PAA, Cölbe, Germany) and adjusted to 6 × 106 cells/ml. PBMCs for each animal were divided into four groups and seeded in 24-well plates at 3 × 106 cells/well, followed by the overnight incubation at 37 °C with 5% CO2. Then, the cells were treated with either 1) vehicle (CON), 2) DEX (5nM in ethanol (≈ 0.002 μg/ml); Dexatat, aniMedica, Senden, Germany), 3) LPS (10 μg/ml in PBS; Escherichia coli O111: B4; Sigma-Aldrich, Taufkirchen, Germany) or 4) combination of LPS+DEX and further incubated for 2 hours at 37 °C with 5% CO2. Afterward, cells were harvested for RNA extraction. Total RNA was extracted from PBMCs using the TRI reagent (Sigma-Aldrich, Taufkirchen, Germany) and purified by the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany) following the manufacturer’s instructions. Genomic DNA was removed by the RNase-Free DNase Set (Qiagen, Hilden, Germany). RNA integrity was assessed by the Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit (Agilent Technologies, Waldbronn, Germany). 1 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture’s recommendation (Illumina).

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 14

characteristic: title


E-MTAB-9808:Sample 2

text: Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 2

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

text: Sample 2


E-MTAB-9808:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 26

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 26


E-MTAB-9808:Sample 38

text: public

text: E-MTAB-9808:Sample 38

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 38

characteristic: title


E-MTAB-9808:Sample 50

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 50

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

text: Sample 50


E-MTAB-9808:Sample 62

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 62

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 62


E-MTAB-9808:Sample 74

text: public

text: E-MTAB-9808:Sample 74

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 74

characteristic: title


E-MTAB-9808:Sample 86

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 86

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

text: Sample 86


E-MTAB-9808:Sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 18


E-MTAB-9808:Sample 30

text: public

text: E-MTAB-9808:Sample 30

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 30

characteristic: title


E-MTAB-9808:Sample 42

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 42

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

text: Sample 42


E-MTAB-9808:Sample 54

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 54

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 54


E-MTAB-9808:Sample 6

text: public

text: E-MTAB-9808:Sample 6

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 6

characteristic: title


E-MTAB-9808:Sample 66

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 66

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

text: Sample 66


E-MTAB-9808:Sample 78

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 78

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 78


E-MTAB-9808:Sample 90

text: public

text: E-MTAB-9808:Sample 90

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 90

characteristic: title


E-MTAB-9808:Sample 10

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 10

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

text: Sample 10


E-MTAB-9808:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 22


E-MTAB-9808:Sample 34

text: public

text: E-MTAB-9808:Sample 34

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 34

characteristic: title


E-MTAB-9808:Sample 46

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 46

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

text: Sample 46


E-MTAB-9808:Sample 58

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 58

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 58


E-MTAB-9808:Sample 70

text: public

text: E-MTAB-9808:Sample 70

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone

text: German Landrace

characteristic: strain

text: Sample 70

characteristic: title


E-MTAB-9808:Sample 82

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 82

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone

text: German Landrace

text: Sample 82


E-MTAB-9808:Sample 94

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 94

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone

characteristic: stimulus

text: German Landrace

text: Sample 94


E-MTAB-9808:Sample 16

text: public

text: E-MTAB-9808:Sample 16

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 16

characteristic: title


E-MTAB-9808:Sample 28

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 28

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 28


E-MTAB-9808:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 4


E-MTAB-9808:Sample 40

text: public

text: E-MTAB-9808:Sample 40

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 40

characteristic: title


E-MTAB-9808:Sample 52

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 52

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 52


E-MTAB-9808:Sample 64

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 64

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 64


E-MTAB-9808:Sample 76

text: public

text: E-MTAB-9808:Sample 76

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 76

characteristic: title


E-MTAB-9808:Sample 88

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 88

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 88


E-MTAB-9808:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 20

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 20


E-MTAB-9808:Sample 32

text: public

text: E-MTAB-9808:Sample 32

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 32

characteristic: title


E-MTAB-9808:Sample 44

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 44

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 44


E-MTAB-9808:Sample 56

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 56

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 56


E-MTAB-9808:Sample 68

text: public

text: E-MTAB-9808:Sample 68

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 68

characteristic: title


E-MTAB-9808:Sample 8

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 8

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 8


E-MTAB-9808:Sample 80

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 80

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 80


E-MTAB-9808:Sample 92

text: public

text: E-MTAB-9808:Sample 92

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 92

characteristic: title


E-MTAB-9808:Sample 12

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 12

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 12


E-MTAB-9808:Sample 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 24

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 24


E-MTAB-9808:Sample 36

text: public

text: E-MTAB-9808:Sample 36

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 36

characteristic: title


E-MTAB-9808:Sample 48

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 48

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 48


E-MTAB-9808:Sample 60

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 60

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 60


E-MTAB-9808:Sample 72

text: public

text: E-MTAB-9808:Sample 72

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: dexamethasone and lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 72

characteristic: title


E-MTAB-9808:Sample 84

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 84

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: dexamethasone and lipopolysaccharide

text: German Landrace

text: Sample 84


E-MTAB-9808:Sample 96

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 96

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: dexamethasone and lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 96


E-MTAB-9808:Sample 15

text: public

text: E-MTAB-9808:Sample 15

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-9808:Sample 27

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 27

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

text: Sample 27


E-MTAB-9808:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 3


E-MTAB-9808:Sample 39

text: public

text: E-MTAB-9808:Sample 39

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 39

characteristic: title


E-MTAB-9808:Sample 51

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 51

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

text: Sample 51


E-MTAB-9808:Sample 63

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 63

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 63


E-MTAB-9808:Sample 75

text: public

text: E-MTAB-9808:Sample 75

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 75

characteristic: title


E-MTAB-9808:Sample 87

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 87

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

text: Sample 87


E-MTAB-9808:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 19


E-MTAB-9808:Sample 31

text: public

text: E-MTAB-9808:Sample 31

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 31

characteristic: title


E-MTAB-9808:Sample 43

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 43

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

text: Sample 43


E-MTAB-9808:Sample 55

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 55

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 55


E-MTAB-9808:Sample 67

text: public

text: E-MTAB-9808:Sample 67

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 67

characteristic: title


E-MTAB-9808:Sample 7

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 7

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

text: Sample 7


E-MTAB-9808:Sample 79

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 79

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 79


E-MTAB-9808:Sample 91

text: public

text: E-MTAB-9808:Sample 91

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 91

characteristic: title


E-MTAB-9808:Sample 11

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 11

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

text: Sample 11


E-MTAB-9808:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 23

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 23


E-MTAB-9808:Sample 35

text: public

text: E-MTAB-9808:Sample 35

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 35

characteristic: title


E-MTAB-9808:Sample 47

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 47

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

text: Sample 47


E-MTAB-9808:Sample 59

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 59

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 59


E-MTAB-9808:Sample 71

text: public

text: E-MTAB-9808:Sample 71

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: lipopolysaccharide

text: German Landrace

characteristic: strain

text: Sample 71

characteristic: title


E-MTAB-9808:Sample 83

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 83

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: lipopolysaccharide

text: German Landrace

text: Sample 83


E-MTAB-9808:Sample 95

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 95

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: lipopolysaccharide

characteristic: stimulus

text: German Landrace

text: Sample 95


E-MTAB-9808:Sample 1

text: public

text: E-MTAB-9808:Sample 1

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-9808:Sample 13

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 13

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

text: Sample 13


E-MTAB-9808:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 25


E-MTAB-9808:Sample 37

text: public

text: E-MTAB-9808:Sample 37

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 37

characteristic: title


E-MTAB-9808:Sample 49

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 49

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

text: Sample 49


E-MTAB-9808:Sample 61

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 61

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: A (Alanine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 61


E-MTAB-9808:Sample 73

text: public

text: E-MTAB-9808:Sample 73

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: A (Alanine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 73

characteristic: title


E-MTAB-9808:Sample 85

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 85

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: A (Alanine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

text: Sample 85


E-MTAB-9808:Sample 17

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 17

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 17


E-MTAB-9808:Sample 29

text: public

text: E-MTAB-9808:Sample 29

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 29

characteristic: title


E-MTAB-9808:Sample 41

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 41

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

text: Sample 41


E-MTAB-9808:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 5


E-MTAB-9808:Sample 53

text: public

text: E-MTAB-9808:Sample 53

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 53

characteristic: title


E-MTAB-9808:Sample 65

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 65

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: H (heterozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

text: Sample 65


E-MTAB-9808:Sample 77

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 77

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: H (heterozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 77


E-MTAB-9808:Sample 89

text: public

text: E-MTAB-9808:Sample 89

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: H (heterozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 89

characteristic: title


E-MTAB-9808:Sample 21

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 21

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

text: Sample 21


E-MTAB-9808:Sample 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 33

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 33


E-MTAB-9808:Sample 45

text: public

text: E-MTAB-9808:Sample 45

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 45

characteristic: title


E-MTAB-9808:Sample 57

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 57

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: vehicle

text: German Landrace

text: Sample 57


E-MTAB-9808:Sample 69

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 69

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 69


E-MTAB-9808:Sample 81

text: public

text: E-MTAB-9808:Sample 81

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: V (Valine homozygote GRAla610Val)

characteristic: individual

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

characteristic: strain

text: Sample 81

characteristic: title


E-MTAB-9808:Sample 9

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-9808:Sample 9

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: pig

characteristic: common name

text: adult

text: V (Valine homozygote GRAla610Val)

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: vehicle

text: German Landrace

text: Sample 9


E-MTAB-9808:Sample 93

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-9808:Sample 93

characteristic: Submitter Id

text: ArrayExpress

text: mononuclear cell

text: pig

text: adult

text: V (Valine homozygote GRAla610Val)

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

characteristic: sex

text: vehicle

characteristic: stimulus

text: German Landrace

text: Sample 93


text: CENTER FOR GENOMIC REGULATION

text: public

text: ArrayExpress

characteristic: cell line

text: precursor B cell

text: human

text: Protocols: Cells were collected at twelve time points after transdifferentiation induction: 0, 3, 6, 9, 12, 18, 24, 36, 48, 72, 120 and 168 hours. Cell pellets were frozen prior to RNA extraction. Human BLaER1 cells were grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS, Gibco), 2 mM L-glutamine, and 100 U/ml penicillin G sodium (Gibco). Cells were maintained at 37ºC in a humid atmosphere containing 5% CO2. Transdifferentiation was induced by addition of 100 nM of beta-estradiol (Calbiochem) and 10 nM of IL-3 and CSF-1 (Peprotech). For the ChIP-seq experiments of the nine histone marks and cEBPalpha, cells were crosslinked with formaldehyde 1 % (Sigma) for 10’ at room temperature. The reaction was stopped by adding glycine to 0.25 M final concentration for 10’ at room temperature. Fixed cells were resuspended in 100 ul of lysis buffer (SDS 1 %, EDTA 10 mM, TrisCl 50 mM and protease inhibitors). The lysate was sonicated for 25’ using Covaris S2 system in TC12 tubes (Duty cycle 20\%, Intensity 8, cycles/burst 200, water level 15). The cleared supernatant was used immediately in ChIP experiments or stored at -80ºC. 5 ug of sonicated chromatin were diluted in 900 ul RIPA buffer - H3K4me3, H3K9ac, H4K20me1, H3K27me3 and H3K27ac (140 mM NaCl, 10 mM Tris HCl pH 8.0, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 0.1 % Na deoxycholate, protease inhibitors) -, RIPA 2X - H3K4me1, H3K4me2 and H3K9me3 (280 mM NaCl, 10 mM Tris HCl pH 8.0, 1 mM EDTA, 2 % Triton X-100, 0.2 % SDS, 0.2 % Na deoxycholate, protease inhibitors) -, or RIPA 1X 1 % triton - H3K36me3 (280 mM NaCl, 10 mM Tris HCl pH 8.0, 1 mM EDTA, 1 % Triton X-100, 0.2 % SDS, 0.2 % Na deoxycholate, protease inhibitors). For H3K4me3, H3K36me3, H3K9ac and H3K27me3 ChIPs, chromatin and antibodies were incubated overnight, rotating at 4ºC with 0.125-5 ug of specific antibody and samples were then incubated for 2 hours rotating at 4ºC with Dynabeads protein A for immunoprecipitation (Invitrogen) to recover the bound material. For H3K4me1, H3K4me2, H3K9me3, H4K20me1 and H3K27ac ChIPs, antibodies were coated to protein A magnetic beads for 2 hours at 4ºC prior to overnight incubation with chromatin. In all cases, beads were washed for 10’ three times in 1 ml corresponding immunoprecipitation buffer without protease inhibitors, then washed once in 1 ml LiCl buffer (0.25 M LiCl, 0.5 % NP-40, 0.5 % sodium deoxycholate, 1 mM Na-EDTA, 10 mM Tris-HCl, pH 8.0) and finally washed twice in 1 ml of TE buffer (1 mM Na-EDTA, 10 mM Tris-HCl, pH 8.0). ChIPped material was incubated with DNase-free RNase at 50 ug/ml for 30’ at 37ºC. Chromatin was reverse-crosslinked by adding SDS (0.5 % final concentration) and Proteinase K (500 ug/ml final concentration) and incubated overnight at 65ºC. ChIPped chromatin was then purified with Qiaquick PCR purification columns (Qiagen) following the manufacturer’s instructions. ChIP libraries were prepared with 1-5 ng of DNA and using NebNext Ultra DNA library prep kit for Illumina (New England Biolabs) according to the manufacturer’s protocol.

characteristic: description

text: chronic lymphocytic leukemia

characteristic: disease

text: C/EBPER-GFP

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: title


text: CENTER FOR GENOMIC REGULATION

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: broker name

text: precursor B cell

text: human

text: chronic lymphocytic leukemia

text: C/EBPER-GFP

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: transdifferentiation to macrophage


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:UNIVERSITY OF COPENHAGEN (SECTION OF ORGANISMAL BIOLOGY)

text: W. Gerlach

characteristic: collected by

characteristic: collection date

text: CBS 189.61

characteristic: culture collection

text: Axenic mono-spore isolate of plant pathogenic fungi

text: France

characteristic: geographic location (country and/or sea)

text: Vosges

characteristic: geographic location (region and locality)

text: Abies alba

characteristic: host scientific name

text: 48.0707, 6.9509

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF COPENHAGEN (SECTION OF ORGANISMAL BIOLOGY)

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: L. Lombard

text: CBS 324.61

characteristic: description

text: Netherlands

text: Zwolle

text: Abies concolor

text: 52.5112, 6.094

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: S. Schmitz

text: Belgium

text: Herbeumont

text: Abies grandis

characteristic: isolate

text: 49.7689, 5.2443

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Switzerland

text: Arboretum

text: Abies nordmaniana

text: 46.5102, 6.3684

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: Robak

text: Norway

characteristic: geographic location (country and/or sea)

text: Os

characteristic: geographic location (region and locality)

text: Abies alba

characteristic: host scientific name

text: NO 1883/5

text: 62.4965, 11.2233

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: R. Roll-Hansen

text: CBS 503.67

characteristic: culture collection

text: Norway

text: Fana

text: Abies alba

text: 60.2741, 5.3954

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: R. Roll-Hansen

characteristic: description

text: Norway

text: Fana

text: Abies alba

text: NO 61-62/1

characteristic: isolate

text: 60.2716, 5.3866

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: J.-O. Skage

text: Norway

text: Fana

text: Abies lasiocarpa

text: NO 252130

text: 60.26, 5.34

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


text: public

text: J.-O. Skage

characteristic: collected by

characteristic: collection date

text: Norway

characteristic: geographic location (country and/or sea)

text: Fana

characteristic: geographic location (region and locality)

text: Abies lasiocarpa

characteristic: host scientific name

text: NO 252140

text: 60.26, 5.34

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Arboretum 1

text: Abies fargesii

text: 55.8691, 12.5033

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Arboretum 1

text: Abies lasiocarpa

text: 55.8667, 12.5097

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Arboretum

text: Abies lasiocarpa

characteristic: isolate

text: 55.8642, 12.5119

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Arboretum 1

text: Abies lasiocarpa

text: 55.8648, 12.5117

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Arboretum

characteristic: geographic location (region and locality)

text: Abies pinsapo

characteristic: host scientific name

text: 55.8642, 12.5093

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Arboretum 1

text: Abies chensiensis

text: 55.8649, 12.5107

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Arboretum 1

text: Abies procera

text: 55.8673, 12.5096

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Silkeborg

text: Abies nordmaniana

characteristic: isolate

text: 56.1627, 9.575

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Silkeborg

text: Abies nordmaniana

text: 56.1632, 9.5757

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Silkeborg

characteristic: geographic location (region and locality)

text: Abies nordmaniana

characteristic: host scientific name

text: 56.1625, 9.5745

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Silkeborg 2

text: Abies nordmaniana

text: 56.1626, 9.5741

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Silkeborg

text: Abies nordmaniana

text: 56.1626, 9.5718

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Silkeborg 2

text: Abies nordmaniana

characteristic: isolate

text: 56.1632, 9.5739

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Thy

text: Abies nordmaniana

text: 57.0242, 8.5987

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Thy

characteristic: geographic location (region and locality)

text: Abies nordmaniana

characteristic: host scientific name

text: 57.0241, 8.5989

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Christiansfeld 3

text: Abies procera

text: 55.3643, 9.4378

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Christiansfeld

text: Abies procera

text: 55.3639, 9.4378

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Christiansfeld

text: Abies procera

characteristic: isolate

text: 55.3637, 9.4379

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Christiansfeld 3

text: Abies procera

text: 55.3632, 9.4378

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Christiansfeld

characteristic: geographic location (region and locality)

text: Abies procera

characteristic: host scientific name

text: 55.363, 9.4378

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Christiansfeld

text: Abies procera

text: 55.3626, 9.4377

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Christiansfeld

text: Abies procera

text: 55.3625, 9.4378

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Christiansfeld

text: Abies procera

characteristic: isolate

text: 55.3621, 9.4376

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Bommerlund

text: Abies nordmaniana

text: 54.879, 9.3447

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Bommerlund

characteristic: geographic location (region and locality)

text: Abies nordmaniana

characteristic: host scientific name

text: 54.8782, 9.3442

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: K.N. Nielsen

text: Denmark

text: Skelhusmarken

text: Abies nordmaniana

text: 56.7781, 9.8417

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: K.N. Nielsen

characteristic: description

text: Denmark

text: Skelhusmarken

text: Abies nordmaniana

text: 56.7789, 9.8423

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K.N. Nielsen

text: Denmark

text: Skelhusmarken

text: Abies nordmaniana

characteristic: isolate

text: 56.779, 9.842

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Denmark

text: Varde

text: Abies grandis

text: 55.5957, 8.5284

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Denmark

characteristic: geographic location (country and/or sea)

text: Varde

characteristic: geographic location (region and locality)

text: Abies grandis

characteristic: host scientific name

text: 55.588, 8.5235

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: A. Uimari

text: Finland

text: Mustila

text: Abies sp.

text: 60.7315, 26.4214

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: A. Uimari

characteristic: description

text: Finland

text: Jarvenpaa

text: Abies sp.

text: 60.4664, 25.0896

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: A. Uimari

text: Finland

text: Abies sp.

characteristic: isolate

text: 60.1919, 24.9368

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: A. Uimari

characteristic: collected by

characteristic: collection date

text: Finland

text: Espoo L 2

text: Abies sp.

text: 60.2014, 24.8041

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: A. Uimari

text: Finland

characteristic: geographic location (country and/or sea)

text: Salo 15

characteristic: geographic location (region and locality)

text: Abies sp.

characteristic: host scientific name

text: 60.3841, 23.0868

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: J. McMinn

text: United Kingdom

text: Llanbrynmair

text: Abies procera

text: 52.5143, -3.6887

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: J. McMinn

characteristic: description

text: United Kingdom

text: Llanbrynmair

text: Abies sp.

text: 52.4674, -3.6808

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: J. McMinn

text: United Kingdom

text: Kent

text: Abies nordmaniana

characteristic: isolate

text: 51.0762, 0.4635

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: J. McMinn

characteristic: collected by

characteristic: collection date

text: Kent

text: Abies sp.

text: 51.0761, 0.4535

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: P.O. Duguay

text: QFB 19253 / CFL 961

characteristic: culture collection

text: Canada

characteristic: geographic location (country and/or sea)

text: Anti-costi Island

characteristic: geographic location (region and locality)

text: Abies balsamea

characteristic: host scientific name

text: 49.82, -64.35

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: P.O. Duguay

text: QFB 19255 / CFL 962

text: Canada

text: Anti-costi Island

text: Abies balsamea

text: 49.8551, -64.1037

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: P.O. Duguay

text: QFB 19262 / CFL 965

characteristic: description

text: Canada

text: Anti-costi Island

text: Abies balsamea

text: 49.8551, -64.1037

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

characteristic: INSDC status

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Canada

text: Anti-costi Island

text: Abies balsamea

characteristic: isolate

text: 49.7493, -63.1044

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Canada

characteristic: geographic location (country and/or sea)

text: Anti-costi Island

characteristic: geographic location (region and locality)

text: Abies balsamea

text: 49.8225, -63.8326

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: K.N. Nielsen

text: Canada

text: Anti-costi Island

text: Abies balsamea

characteristic: host scientific name

text: 49.7564, -63.0759

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

text: K.N. Nielsen

text: Canada

text: Anti-costi Island

text: Abies balsamea

text: 49.8266, -63.8492

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: K.N. Nielsen

characteristic: description

text: Canada

text: Anti-costi Island

text: Abies balsamea

text: 49.5589, -62.6887

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

characteristic: collection date

text: Canada

text: Anti-costi Island

text: Abies balsamea

characteristic: isolate

text: 49.7563, -63.0761

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

text: public

text: K.N. Nielsen

text: Canada

characteristic: geographic location (country and/or sea)

text: Anti-costi Island

characteristic: geographic location (region and locality)

text: Abies balsamea

text: 49.8224, -63.8323

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: K.N. Nielsen

text: Canada

text: Anti-costi Island

text: Abies balsamea

characteristic: host scientific name

text: 49.9146, -64.341

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

text: K.N. Nielsen

text: Canada

text: Anti-costi Island

text: Abies balsamea

text: 49.915, -64.3394

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: CFL 963

characteristic: culture collection

characteristic: description

text: Canada

text: Port Cartier

text: Abies balsamea

text: 50.03, -66.87

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

text: public

characteristic: collection date

text: CFL 964

text: Canada

text: Baie-Comeau

text: Abies balsamea

text: 49.23, -68.16

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

text: Canada

characteristic: geographic location (country and/or sea)

text: Cote Nord

characteristic: geographic location (region and locality)

text: Abies balsamea

characteristic: isolate

text: 50.2998, -66.4364

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: S. Shamoun

text: CBS 118985

text: Canada

text: British Columbia

text: Tsuga heterophylla

characteristic: host scientific name

text: 52, -126

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: K.N. Nielsen

text: Canada

text: Cobble Hill

text: Abies lasiocarpa

text: 48.7002, -123.5912

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

text: K.N. Nielsen

characteristic: collection date

characteristic: description

text: Canada

text: Cobble Hill

text: Abies lasiocarpa

text: 48.7005, -123.5911

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

text: K.N. Nielsen

characteristic: collected by

text: Canada

characteristic: geographic location (country and/or sea)

text: Cobble Hill

characteristic: geographic location (region and locality)

text: Abies lasiocarpa

text: 48.7004, -122.591

text: Neonectria neomacrospora

characteristic: organism

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: K.N. Nielsen

text: Canada

text: Cobble Hill

text: Abies lasiocarpa

characteristic: isolate

text: 48.7002, -123.591

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: L. Reitman

text: CBS 118984

characteristic: culture collection

text: Canada

text: Vancouver Island

text: Arceuthobium tsugensw. on

characteristic: host scientific name

text: 49.3471, -124.6259

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Zhao-Qing Zeng

text: HMAS 252906

text: China

text: Shennongjia

text: Pinus sp.

text: 31.73, 110.68

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


text: public

characteristic: INSDC status

tag: Namespace:Children’s Brain Tumour Research Centre, School of Medicine; Biodiscovery Institute, University of Nottingham, Nottingham, UK

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Daoy

characteristic: cell line

text: human

characteristic: common name

text: Protocols: Cell pellets of at least 100,000 cells were washed with HBSS and frozen in liquid nitrogen. Medulloblastoma cell lines representating the different subgroups have been cultured and cell were harvested and RNA was isolated when 70% confluency was reached. In detail, DAOY and D458 were grown in DMEM (Thermo Fisher) with 10% FBS (HyClone, Thermo Fisher), ONS-76 and HD-MB03 were grown in RPMI 1640 (Sigma-Aldrich) with 10% FBS and CHLA-01-MED and CHLA-01R-MED were grown in DMEMF12 supplemented with B27, 20 ng/ml EGF and 20 ng/ml bFGF (all Thermo Fisher). All cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2. During the course of this study, all cell lines were routinely confirmed to be mycoplasma negative (MycoAlert, Lonza, Basel, Switzerland). For homogenization, ceramic spheres (Lysing Matrix D, MP Biomedicals, Santa Ana, California, USA) and the FastPrep-24 homogenizer was used (MP Biomedicals, speed 4 m/s, tube holder MP:24*2 and time 20 s). Total RNA was isolated from 2D pellets using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. In total, 3 biological replicates of each cell line were processed respectively. RNA amount was determined using the Qubit RNA BR kit with the Qubit 4 (both ThermoFisher). Library preparation/sequencing/analysis were performed by Novogene (UK) Company Limited. RNA sample was used for library preparation. Ribosomal RNA was firstly removed. Then the directional sequencing library was constructed using NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s protocol. Indices were included to multiplex multiple samples. Briefly, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The strand-specific library was ready after end repair, A-tailing, adapter ligation, size selection and USER enzyme digestion. After amplification and purification, insert size of the library was validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR).

characteristic: description

text: medulloblastoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

characteristic: replicate

text: Homo sapiens