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characteristic: collecting institution

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text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: Samples prepared at Lighthouse Lab in Milton Keynes by The Lighthouse Lab in Milton Keynes and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human

characteristic: organism


text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

text: Lighthouse Laboratory Plymouth

characteristic: description

text: United Kingdom

text: Wales

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human

characteristic: title


text: SC

text: public

text: SC

characteristic: broker name

text: Lighthouse Lab in Alderley Park

characteristic: collecting institution

characteristic: collection date

text: Samples prepared at Lighthouse Lab in Alderley Park by Jacquelyn Wynn, Mairead Hyland, The Lighthouse Lab in Alderley Park and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: Human


characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: United Kingdom

text: Scotland

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

characteristic: sample capture status


text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SC

text: Lighthouse Lab in Alderley Park

text: United Kingdom

text: Wales

text: Human

characteristic: organism


text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

text: Samples prepared at Berkshire and Surrey Pathology Services Lighthouse Laboratory by Berkshire and Surrey Pathology Services Lighthouse Laboratory and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

characteristic: description

text: United Kingdom

text: England

text: Human


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: SC

text: Berkshire and Surrey Pathology Services Lighthouse Laboratory

text: United Kingdom

text: England

text: Human

characteristic: title


text: SC

text: public

text: SC

characteristic: broker name

text: Lighthouse Lab in Milton Keynes

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

text: Human


characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Lab in Milton Keynes

text: United Kingdom

text: Scotland

characteristic: geographic location (region and locality)

text: Human

characteristic: host scientific name

characteristic: sample capture status


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:VIB-Ugent center for plant systems biology

text: 2 days after leaf 4 appearance

characteristic: age

text: ArrayExpress

characteristic: cultivar

text: Protocols: Three zones were sampled, from the base to 3.5 cm, from 3.5 to 7.0 cm and from 7.0 to 10.5 cm, of the developing fourth leaf of maize B104 plants at two days after leaf appearance (DALA). Only leaf samples were taken from well-watered plants. To sample the three zones of a developing fourth leaf two days after leaf emergence, the older leaves that surround the growing leaf should be removed. Special care should be taken not to damage the basal part of the growing leaf because this is where the leaf basal meristem is located. Subsequently the fourth leaf was cut into three parts corresponding to the three sampled zones. B104 seeds were sown in soil and grown in a controlled-environment growth chamber (relative humidity (55%), temperature (24 °C day/18 °C night) and 16 h/8 h (day/night) cycle). Only well-watered plants. Purified poly(A)-containing mRNA molecules were fragmented, randomly primed strand-specific cDNA was generated and adapters were ligated. Total RNA was extracted using the guanidinium thiocyanate-phenol-chloroform extraction method using TRI-reagent (Thermo Fisher Scientific) followed by DNA digestion using the RQ1 RNase-free DNase kit (Promega). Library preparation was done using the NEBNext Kit (Illumina).

text: LP.04 four leaves visible stage

characteristic: developmental stage

text: well watered

characteristic: environmental history

text: wild type genotype

characteristic: genotype

text: growth chamber study

characteristic: growth condition

text: Zea mays

characteristic: organism

text: Zea mays

text: leaf

characteristic: organism part

text: leaf 4, cm 0 - 3.5 measured from the base of the leaf. Division and expansion zone.

characteristic: sampling site

text: Zea mays


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VIB-Ugent center for plant systems biology

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 2 days after leaf 4 appearance

text: ArrayExpress

characteristic: broker name

characteristic: description

text: LP.04 four leaves visible stage

text: well watered

text: wild type genotype

text: growth chamber study

text: Zea mays

text: Zea mays

text: leaf

text: Zea mays

characteristic: title


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Anthony Smith, NICD

text: EnteroBase

text: EnteroBase

text: A. Smith, National Institute for Communicable Diseases

characteristic: collected by

characteristic: collection date

text: Whole genome sequencing data uploaded by EnteroBase user

text: South Africa, Gauteng

characteristic: geographic location

text: Human, Blood culture

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Typhi

characteristic: serovar

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Eastern Cape

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Muenchen


text: Anthony Smith, NICD

text: public

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characteristic: SRA accession

characteristic: Submitter Id

text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Eastern Cape

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa, Eastern Cape

text: Human, Blood culture

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium

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text: Anthony Smith, NICD

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characteristic: INSDC last update

text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

characteristic: collection date

text: South Africa, Eastern Cape

text: Human, Blood culture

text: Salmonella enterica

text: Salmonella enterica


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, KwaZulu-Natal

characteristic: geographic location

text: Human, Blood culture

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text: Salmonella enterica

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text: Enteritidis

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characteristic: ENA-LAST-UPDATE

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, KwaZulu-Natal

text: Human, Other

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Dublin


text: Anthony Smith, NICD

text: public

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text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Western Cape

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text: Anthony Smith, NICD

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text: EnteroBase

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characteristic: description

text: South Africa, Eastern Cape

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text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

characteristic: collection date

text: South Africa, Eastern Cape

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text: Salmonella enterica

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text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Eastern Cape

characteristic: geographic location

text: Human, Urine

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text: Anthony Smith, NICD

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text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Eastern Cape

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text: Chester


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text: EnteroBase

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text: South Africa, Eastern Cape

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text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

characteristic: collection date

text: South Africa, Western Cape

text: Human, Blood culture

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Gauteng

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text: Human, Blood culture

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text: Salmonella enterica

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text: Enteritidis

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text: Anthony Smith, NICD

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text: South Africa, North West

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text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Gauteng

text: Human, Stool

text: Salmonella enterica

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text: Enteritidis


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa, Gauteng

text: Human, Blood culture

text: Salmonella enterica

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characteristic: title


text: Anthony Smith, NICD

characteristic: INSDC first public

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text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

characteristic: collection date

text: South Africa, KwaZulu-Natal

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Gauteng

characteristic: geographic location

text: Human, Other

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Dublin

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Western Cape

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica


text: Anthony Smith, NICD

text: public

characteristic: INSDC status

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characteristic: Submitter Id

text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Gauteng

text: Human, Blood culture

text: Salmonella enterica

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text: Typhimurium


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa, Gauteng

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium

characteristic: title


text: Anthony Smith, NICD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

characteristic: collection date

text: South Africa, Eastern Cape

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, KwaZulu-Natal

characteristic: geographic location

text: Human, Stool

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text: Salmonella enterica

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text: Hadar

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characteristic: ENA-FIRST-PUBLIC

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text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Free State

text: Human, Stool

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text: Salmonella enterica

text: Infantis


text: Anthony Smith, NICD

text: public

characteristic: INSDC status

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text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, Western Cape

text: Human, Stool

text: Salmonella enterica

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text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa, Western Cape

text: Human, Stool

text: Salmonella enterica

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text: Braenderup

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text: Anthony Smith, NICD

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text: public

text: EnteroBase

text: EnteroBase

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characteristic: collection date

text: South Africa, KwaZulu-Natal

text: Human, Blood culture

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text: Enteritidis


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, KwaZulu-Natal

characteristic: geographic location

text: Human, Stool

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characteristic: ENA-LAST-UPDATE

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Mpumalanga

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text: Salmonella enterica

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text: Salmonella enterica

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text: Anthony Smith, NICD

text: public

characteristic: INSDC status

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text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa

text: Human, Stool

text: Salmonella enterica

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text: Typhimurium


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa

text: Human, Blood culture

text: Salmonella enterica

text: Salmonella enterica

text: Carno

characteristic: title


text: Anthony Smith, NICD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: EnteroBase

text: EnteroBase

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characteristic: collection date

text: South Africa

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa

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text: Human, Blood culture

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text: Salmonella enterica

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, North West

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Typhimurium


text: Anthony Smith, NICD

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: EnteroBase

characteristic: broker name

text: EnteroBase

text: South Africa, KwaZulu-Natal

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium


characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

text: EnteroBase

text: EnteroBase

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text: South Africa, Western Cape

text: Human, Urine

text: Salmonella enterica

text: Salmonella enterica

text: Manhattan

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text: Anthony Smith, NICD

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text: public

text: EnteroBase

text: EnteroBase

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characteristic: collection date

text: South Africa, Gauteng

text: Human, Blood culture

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text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, North West

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text: Human, Stool

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text: Anthony Smith, NICD

text: public

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text: EnteroBase

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text: Anthony Smith, NICD

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text: EnteroBase

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text: South Africa, Western Cape

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text: Anthony Smith, NICD

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text: public

text: EnteroBase

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text: South Africa, Western Cape

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text: public

text: EnteroBase

text: EnteroBase

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text: South Africa, Western Cape

text: Human, Other

text: Salmonella enterica

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text: Anthony Smith, NICD

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text: EnteroBase

text: EnteroBase

characteristic: collection date

text: South Africa, Western Cape

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text: Human, Blood culture

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text: Anthony Smith, NICD

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text: EnteroBase

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text: Anthony Smith, NICD

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characteristic: INSDC status

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text: EnteroBase

text: EnteroBase

text: South Africa, Gauteng

text: Human, Stool

text: Salmonella enterica

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text: Salmonella enterica

text: Isangi


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text: Anthony Smith, NICD

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text: public

text: EnteroBase

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text: EnteroBase

characteristic: description

text: South Africa, Western Cape

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text: Salmonella enterica

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text: Typhimurium


text: Anthony Smith, NICD

characteristic: INSDC first public

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text: public

text: EnteroBase

text: EnteroBase

characteristic: collected by

text: South Africa, Northern Cape

text: Human, Other

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis

characteristic: title


text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

characteristic: collection date

text: South Africa, Gauteng

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text: Human, Stool

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text: Typhimurium

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text: Anthony Smith, NICD

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text: EnteroBase

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text: Anthony Smith, NICD

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text: EnteroBase

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text: EnteroBase

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text: South Africa, Western Cape

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text: Salmonella enterica

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text: public

text: EnteroBase

text: EnteroBase

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text: South Africa, Free State

text: Human, Stool

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text: Enteritidis

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text: Anthony Smith, NICD

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text: EnteroBase

text: EnteroBase

characteristic: collection date

text: South Africa, Free State

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text: Human, Stool

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text: Anthony Smith, NICD

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text: EnteroBase

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text: South Africa, Eastern Cape

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text: Salmonella enterica

text: Typhimurium

characteristic: strain