text: public

tag: Namespace:Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: common name

text: Protocols: MEC1 cells were cultured in RPMI-1640 medium with L-glutamine and sodium bicarbonide enriched with 10% Fetal Bovine Serum (FBS) Superior and 15μg/ml gentamicin at a density of 0.6x10^6 cells/ml and maintained at 37⁰C and 5% CO2 in a humidified atmosphere. Cells were fixed with 1% formaldehyde for 10 minutes at RT, quenched with glycine and lysed using cell and nuclei lysis buffers. Chromatin was extracted and sonicated to a range of 200-1000 bp using a Covaris S220 sonicator.For the immunoprecipitation step, chromatin was precleared with 20 μl magnetic protein G beads per sample for 1h at 4°C in a rotating wheel and then incubated with monoclonal TP63 antibody. NGS libraries were prepared according to a previously published protocol (Ford et al., 2014, DOI: 10.1186/1756-0500-7-312), modified with the addition of the NEBNext Multiplex Oligos for Illumina.

characteristic: description

text: B-cell chronic lymphocytic leukemia

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: B-cell chronic lymphocytic leukemia

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: public

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: cell type

text: human

text: Protocols: Primary CLL cells were cultured in RPMI-1640 medium with L-glutamine and sodium bicarbonide enriched with 10% Fetal Bovine Serum (FBS) Superior and 15μg/ml gentamicin at a density of 3x10^6 cells/ml and maintained at 37⁰C and 5% CO2 in a humidified atmosphere. Cells were fixed with 1% formaldehyde for 10 minutes at RT, quenched with glycine and lysed using cell and nuclei lysis buffers. Chromatin was extracted and sonicated to a range of 200-1000 bp using a Covaris S220 sonicator.For the immunoprecipitation step, chromatin was precleared with 20 μl magnetic protein G beads per sample for 1h at 4°C in a rotating wheel and then incubated with monoclonal TP63 antibody. NGS libraries were prepared according to a previously published protocol (Ford et al., 2014, DOI: 10.1186/1756-0500-7-312), modified with the addition of the NEBNext Multiplex Oligos for Illumina.

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: male

characteristic: sex


text: public

text: E-MTAB-10717:TP63_ChIP-seq_primary_CLL_cells

text: ArrayExpress

text: B cell

text: human

characteristic: common name

characteristic: description

text: adult

text: chronic lymphocytic leukemia

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: male


E-MTAB-10726:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10726:Sample 5

text: ArrayExpress

characteristic: cell line

text: house mouse

text: Protocols: CT26 with TRE-GNA13 Q226L transgene were untreated or treated with 100 ng/mL doxycycline for 48h Qiagen RNeasy mini NEBNext® Ultra™ RNA Library Prep Kit for Illumina

text: adult

text: carcinoma

text: TRE-GNA13 Q226L

text: Mus musculus

text: Mus musculus

text: large intestine

characteristic: organism part

text: doxycyclin mediated expression of TRE-GNA13 Q226L

characteristic: phenotype

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 5


E-MTAB-10726:Sample 2

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10726:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: carcinoma

text: TRE-GNA13 Q226L

text: Mus musculus

text: Mus musculus

text: large intestine

text: wild type phenotype

text: Mus musculus

text: BALB/c

text: Sample 2

characteristic: title


E-MTAB-10726:Sample 1

text: HMS

text: public

text: E-MTAB-10726:Sample 1

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: carcinoma

characteristic: disease

text: TRE-GNA13 Q226L

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: large intestine

text: wild type phenotype

text: Mus musculus

text: BALB/c

text: Sample 1


E-MTAB-10726:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10726:Sample 6

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: carcinoma

text: TRE-GNA13 Q226L

text: Mus musculus

text: Mus musculus

text: large intestine

text: Mus musculus

text: BALB/c

text: Sample 6


E-MTAB-10726:Sample 3

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10726:Sample 3

text: ArrayExpress

text: house mouse

text: adult

text: carcinoma

text: TRE-GNA13 Q226L

text: Mus musculus

text: Mus musculus

text: large intestine

characteristic: organism part

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 3


E-MTAB-10726:Sample 4

text: HMS

text: public

characteristic: SRA accession

text: E-MTAB-10726:Sample 4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: carcinoma

text: TRE-GNA13 Q226L

text: Mus musculus

text: Mus musculus

text: large intestine

text: Mus musculus

text: BALB/c

text: Sample 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: ArrayExpress

text: house mouse

text: Protocols: HLA and SIINFEKL negative cells were sorted after interferon gamma stimulation or HLA and SIINFEKL positive cells were sorted without treatment phenol chloroform custom primers

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CRISPR library targeting TSGs

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: HLA input

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10723:B6_HLA_negative_Rep1_PLUS_IFN

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: HLA negative

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus


text: HMS

text: public

characteristic: SRA accession

text: E-MTAB-10723:B6_HLA_negative_Rep2_PLUS_IFN

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: HLA negative

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10723:B6_HLA_negative_Rep3_PLUS_IFN

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CRISPR library targeting TSGs

characteristic: genetic modification

text: wild type genotype

text: HLA negative

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10723:B6_HLA_positive_Rep1_NO_IFN

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

characteristic: genotype

text: HLA positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus


text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10723:B6_HLA_positive_Rep2_NO_IFN

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: HLA positive

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10723:B6_HLA_positive_Rep3_NO_IFN

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: HLA positive

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CRISPR library targeting TSGs

characteristic: genetic modification

text: wild type genotype

text: SIINFEKL input

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: title


text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10723:B6_SIINFEKL_negative_Rep1_PLUS_IFN

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

characteristic: genotype

text: SIINFEKL negative

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10723:B6_SIINFEKL_negative_Rep2_PLUS_IFN

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: SIINFEKL negative

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10723:B6_SIINFEKL_negative_Rep3_PLUS_IFN

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: SIINFEKL negative

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: strain


text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10723:B6_SIINFEKL_positive_Rep1_NO_IFN

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CRISPR library targeting TSGs

characteristic: genetic modification

text: wild type genotype

text: SIINFEKL positive

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10723:B6_SIINFEKL_positive_Rep2_NO_IFN

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

characteristic: genotype

text: SIINFEKL positive

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10723:B6_SIINFEKL_positive_Rep3_NO_IFN

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: adult

text: normal

text: CRISPR library targeting TSGs

text: wild type genotype

text: SIINFEKL positive

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus


E-MTAB-10725:Sample 31

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 31

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

characteristic: common name

text: Protocols: KP1233 cells with CRISPR library were grown in vitro for 10 PDs or as tumors in Rag1-null or immunocompetent WT C57BL/6 mice phenol chloroform custom primers

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 31


E-MTAB-10725:Sample 32

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 32

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 32

characteristic: title


E-MTAB-10725:Sample 33

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 33

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 33


E-MTAB-10725:Sample 34

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 34

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 34


E-MTAB-10725:Sample 35

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 35

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 35


E-MTAB-10725:Sample 36

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 36

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 36

characteristic: title


E-MTAB-10725:Sample 15

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 15

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 15


E-MTAB-10725:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10725:Sample 16

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 16


E-MTAB-10725:Sample 17

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 17

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 17


E-MTAB-10725:Sample 18

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 18

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 18

characteristic: title


E-MTAB-10725:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 19

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 19


E-MTAB-10725:Sample 20

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 20

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 20


E-MTAB-10725:Sample 21

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 21

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 21


E-MTAB-10725:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10725:Sample 22

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 22

characteristic: title


E-MTAB-10725:Sample 23

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 23

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 23


E-MTAB-10725:Sample 24

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 24

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 24


E-MTAB-10725:Sample 25

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 25

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 25


E-MTAB-10725:Sample 26

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 26

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 26

characteristic: title


E-MTAB-10725:Sample 27

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 27

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 27


E-MTAB-10725:Sample 28

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10725:Sample 28

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 28


E-MTAB-10725:Sample 29

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 29

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 29


E-MTAB-10725:Sample 30

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 30

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 30

characteristic: title


E-MTAB-10725:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 1

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 1


E-MTAB-10725:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 2


E-MTAB-10725:Sample 3

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 3

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 3


E-MTAB-10725:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10725:Sample 4

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 4

characteristic: title


E-MTAB-10725:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 5

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 5


E-MTAB-10725:Sample 6

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 6


E-MTAB-10725:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 7

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 7


E-MTAB-10725:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 8

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 8

characteristic: title


E-MTAB-10725:Sample 9

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 9

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 9


E-MTAB-10725:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

text: E-MTAB-10725:Sample 10

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 10


E-MTAB-10725:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10725:Sample 11

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

characteristic: strain

text: Sample 11


E-MTAB-10725:Sample 12

text: HMS

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10725:Sample 12

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: lung cancer

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 12

characteristic: title


E-MTAB-10725:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10725:Sample 13

text: ArrayExpress

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: Sample 13


E-MTAB-10725:Sample 14

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

text: E-MTAB-10725:Sample 14

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

text: lung cancer

text: CRISPR library against TSGs

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: Sample 14


text: HMS BWH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:HMS BWH

text: ArrayExpress

text: coculture 1 to 10 with OT-I CD8 T cell

characteristic: coculture

text: house mouse

characteristic: common name

text: Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon

characteristic: description

text: adult

text: OVA full length

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: HMS BWH

text: public

characteristic: INSDC status

text: ArrayExpress

text: coculture 1 to 4 with OT-I CD8 T cell

text: house mouse

text: adult

characteristic: developmental stage

text: OVA full length

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS BWH

text: public

characteristic: SRA accession

text: ArrayExpress

text: monoculture

text: house mouse

text: adult

text: OVA full length

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus


E-MTAB-10727:Sample 1

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10727:Sample 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: Protocols: CT26 with expressing Cas9 and either control or Gna13 targeting sgRNAs (see additional file: TSG CRISPR library.txt, Gna13.3 and Gna13.4) were selected with puromycin. Following selection cells were isolated for RNA-seq Qiagen RNeasy mini NEBNext® Ultra™ RNA Library Prep Kit for Illumina

text: adult

text: colon carcinoma

characteristic: disease

text: scrambled guide RNA

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: BALB/c

text: Sample 1


E-MTAB-10727:Sample 4

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10727:Sample 4

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 4


E-MTAB-10727:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: HMS

text: public

characteristic: INSDC status

text: E-MTAB-10727:Sample 2

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

text: Sample 2

characteristic: title


E-MTAB-10727:Sample 3

text: HMS

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-10727:Sample 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

text: Sample 3


E-MTAB-10727:Sample 5

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10727:Sample 5

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 3

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: BALB/c

text: Sample 5


E-MTAB-10727:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10727:Sample 6

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 6


E-MTAB-10727:Sample 7

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10727:Sample 7

text: ArrayExpress

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

text: Sample 7

characteristic: title


E-MTAB-10727:Sample 8

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10727:Sample 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: BALB/c

text: Sample 8


E-MTAB-10727:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10727:Sample 9

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 4

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: BALB/c

text: Sample 9


E-MTAB-10737:Sample 1

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 1

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: CT26 with expressing Cas9 and either control or Gna13 targeting sgRNAs (see additional file: TSG CRISPR library.txt, Gna13.3 and Gna13.4) were selected with puromycin. Cells were implanted into immunocompetent BALB/c mice. Following tumor formation, cells were isolated for RNA-seq Qiagen RNeasy mini NEBNext® Ultra™ RNA Library Prep Kit for Illumina

characteristic: description

text: adult

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

text: implanted in immunocompetent BALB/c mice

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 1


E-MTAB-10737:Sample 3

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 3

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 3

characteristic: title


E-MTAB-10737:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 2

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: scrambled guide RNA

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 2


E-MTAB-10737:Sample 4

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 4

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 4


E-MTAB-10737:Sample 5

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: scrambled guide RNA

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 5

characteristic: title


E-MTAB-10737:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 6

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 3

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 6


E-MTAB-10737:Sample 7

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 7

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 7


E-MTAB-10737:Sample 8

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 8

characteristic: title


E-MTAB-10737:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 9

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 3

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 9


E-MTAB-10737:Sample 10

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 10

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 10


E-MTAB-10737:Sample 11

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 11

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: Gna13 targeting sgRNA number 3

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 11

characteristic: title


E-MTAB-10737:Sample 12

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 12

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 4

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 12


E-MTAB-10737:Sample 13

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 13

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 13


E-MTAB-10737:Sample 14

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 14

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 14

characteristic: title


E-MTAB-10737:Sample 15

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 15

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 4

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 15


E-MTAB-10737:Sample 16

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10737:Sample 16

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

characteristic: sampling site

text: Mus musculus

text: BALB/c

characteristic: strain

text: Sample 16


E-MTAB-10737:Sample 17

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10737:Sample 17

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

characteristic: developmental stage

text: colon carcinoma

text: Gna13 targeting sgRNA number 4

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: colon

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 17

characteristic: title


E-MTAB-10737:Sample 18

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10737:Sample 18

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

text: colon carcinoma

characteristic: disease

text: Gna13 targeting sgRNA number 4

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

characteristic: organism part

text: neoplasm

text: Mus musculus

text: BALB/c

text: Sample 18


E-MTAB-10729:Sample 29

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 29

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: 185-3 MPNST cells with CRISPR library were grown in vitro for 10 PDs or as tumors in Rag1-null or immunocompetent WT C57BL/6 mice. 185-3 cells are from a collaborator (Karen Cichowski). They were derived from a mouse tumor model of MPNST which is described here: Cichowski K, Shih TS, Schmitt E, Santiago S, Reilly K, McLaughlin ME, Bronson RT, Jacks T. Mouse models of tumor development in neurofibromatosis type 1. Science. 1999 Dec 10;286(5447):2172-6. doi: 10.1126/science.286.5447.2172. PMID: 10591652. phenol chloroform custom primers

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: in vitro

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 29


E-MTAB-10729:Sample 30

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 30

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: in vitro

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 30


E-MTAB-10729:Sample 31

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 31

text: ArrayExpress

characteristic: cell line

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: in vitro

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 31

characteristic: title


E-MTAB-10729:Sample 32

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 32

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: in vitro

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 32


E-MTAB-10729:Sample 33

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 33

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: in vitro

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 33


E-MTAB-10729:Sample 34

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 34

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: in vitro

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 34


E-MTAB-10729:Sample 14

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 14

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: injection until tumour growth in Rag1 -/- mice

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 14

characteristic: title


E-MTAB-10729:Sample 15

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 15

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 15


E-MTAB-10729:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 16

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 16


E-MTAB-10729:Sample 17

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 17

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 17


E-MTAB-10729:Sample 18

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 18

characteristic: title


E-MTAB-10729:Sample 19

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 19

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 19


E-MTAB-10729:Sample 1

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 1

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: injection until tumour growth in wild type genotype mice

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 1


E-MTAB-10729:Sample 10

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 10


E-MTAB-10729:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 11

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 11

characteristic: title


E-MTAB-10729:Sample 12

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 12

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 12


E-MTAB-10729:Sample 13

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 13

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 13


E-MTAB-10729:Sample 20

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 20

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 20


E-MTAB-10729:Sample 21

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 21

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 21

characteristic: title


E-MTAB-10729:Sample 22

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 22


E-MTAB-10729:Sample 23

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 23

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 23


E-MTAB-10729:Sample 24

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 24

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 24


E-MTAB-10729:Sample 25

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 25

characteristic: title


E-MTAB-10729:Sample 26

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 26

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 26


E-MTAB-10729:Sample 27

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 27

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 27


E-MTAB-10729:Sample 28

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 28

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 28


E-MTAB-10729:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 2

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 2

characteristic: title


E-MTAB-10729:Sample 3

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 3

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 3


E-MTAB-10729:Sample 4

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 4


E-MTAB-10729:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 5

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 5


E-MTAB-10729:Sample 6

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 6

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 6

characteristic: title


E-MTAB-10729:Sample 7

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10729:Sample 7

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: embryo

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

text: Mus musculus

characteristic: organism

text: Mus musculus

text: inner cell mass

characteristic: organism part

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 7


E-MTAB-10729:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10729:Sample 8

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: embryo

characteristic: developmental stage

text: malignant peripheral nerve sheath tumor

text: CRISPR library against TSGs

text: Nf1 +/−; p53 +/−

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

characteristic: strain

text: Sample 8


E-MTAB-10729:Sample 9

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10729:Sample 9

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: embryo

text: malignant peripheral nerve sheath tumor

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: Nf1 +/−; p53 +/−

text: Mus musculus

text: Mus musculus

text: inner cell mass

text: Mus musculus

text: 129/sv × C57BL/6

text: Sample 9


E-MTAB-10728:Sample 36

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 36

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Cells infected with a CRISPR library targeting TSGs were grown in vitro or implanted into immunodeficient or immunocompetent mice. phenol chloroform custom primers

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: in vitro

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 36

characteristic: title


E-MTAB-10728:Sample 40

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 40

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: in vitro

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 40


E-MTAB-10728:Sample 41

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 41

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: in vitro

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 41


E-MTAB-10728:Sample 1

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 1

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 1


E-MTAB-10728:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 2

characteristic: title


E-MTAB-10728:Sample 3

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 3

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 3


E-MTAB-10728:Sample 4

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 4

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 4


E-MTAB-10728:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 5

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 5


E-MTAB-10728:Sample 6

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 6

characteristic: title


E-MTAB-10728:Sample 37

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 37

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: in vitro

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 37


E-MTAB-10728:Sample 38

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 38

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: in vitro

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 38


E-MTAB-10728:Sample 39

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 39

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: in vitro

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 39


E-MTAB-10728:Sample 19

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 19

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 19

characteristic: title


E-MTAB-10728:Sample 20

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 20

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 20


E-MTAB-10728:Sample 21

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 21

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 21


E-MTAB-10728:Sample 22

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 22

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 22


E-MTAB-10728:Sample 23

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 23

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 23

characteristic: title


E-MTAB-10728:Sample 24

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 24

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 24


E-MTAB-10728:Sample 10

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 10

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 10


E-MTAB-10728:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 11

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 11


E-MTAB-10728:Sample 12

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 12

characteristic: title


E-MTAB-10728:Sample 7

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 7

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 7


E-MTAB-10728:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 8

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 8


E-MTAB-10728:Sample 9

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 9

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 9


E-MTAB-10728:Sample 25

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 25

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 25

characteristic: title


E-MTAB-10728:Sample 26

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 26

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 26


E-MTAB-10728:Sample 27

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 27

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 27


E-MTAB-10728:Sample 28

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 28

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 28


E-MTAB-10728:Sample 29

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 29

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 29

characteristic: title


E-MTAB-10728:Sample 30

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 30

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 30


E-MTAB-10728:Sample 13

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 13

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 13


E-MTAB-10728:Sample 14

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 14

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 14


E-MTAB-10728:Sample 15

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

text: E-MTAB-10728:Sample 15

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 15

characteristic: title


E-MTAB-10728:Sample 16

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 16

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 16


E-MTAB-10728:Sample 17

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 17

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 17


E-MTAB-10728:Sample 18

characteristic: External Id

text: HMS

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 18

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 18


E-MTAB-10728:Sample 31

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

text: E-MTAB-10728:Sample 31

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 31

characteristic: title


E-MTAB-10728:Sample 32

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 32

text: ArrayExpress

characteristic: cell line

text: house mouse

text: adult

characteristic: developmental stage

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: skin

characteristic: organism part

text: Mus musculus

text: Sample 32


E-MTAB-10728:Sample 33

characteristic: External Id

text: HMS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-10728:Sample 33

text: ArrayExpress

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 33


E-MTAB-10728:Sample 34

characteristic: ENA-CHECKLIST

text: HMS

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10728:Sample 34

text: ArrayExpress

text: house mouse

text: adult

text: melanoma

characteristic: disease

text: CRISPR library against TSGs

characteristic: genetic modification

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 34


E-MTAB-10728:Sample 35

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: HMS

text: public

text: E-MTAB-10728:Sample 35

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

text: adult

text: melanoma

text: CRISPR library against TSGs

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: skin

text: Mus musculus

text: Sample 35

characteristic: title


E-MTAB-10734:Sample 3

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10734:Sample 3

tag: Namespace:Kennedy Institute of Rheumatology University of Oxford

unit: week

characteristic: age

text: ArrayExpress

text: bone marrow macrophage

characteristic: cell type

text: house mouse

text: Protocols: CSF1-cultured macrophages were collected and sorted into CD11b+CD206high populations which then were separated by each genotype based on the expression of CD45.1 or CD45.2. Bone marrow cells from CD45.1 and Clec4a2-/- (CD45.2) mice were co-cultured with 100 ng/ml CSF1 for 10 days. Total RNA was isolated by using the RNeasy micro kit (Qiagen). Total RNA quantity and integrity were assessed using Quant-IT RiboGreen RNA Assay Kit (ThermoFisher Scientific) and high-sensitivity DNA tapestation (Agilent). Purification of mRNA, generation of double stranded cDNA and library construction were performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina with adapters and barcode tags (dual indexing). The final size distribution of the pool was determined using high-sensitivity DNA tapestation (Agilent), and quantification was determined by Quant-IT RiboGreen RNA Assay Kit (ThermoFisher Scientific).

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 3


E-MTAB-10734:Sample 5

characteristic: ENA-CHECKLIST

text: Kennedy Institute of Rheumatology University of Oxford

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10734:Sample 5

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Clec4a2 -/-

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

text: Sample 5


E-MTAB-10734:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10734:Sample 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

text: house mouse

text: adult

text: wild type genotype

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

text: Sample 1

characteristic: title


E-MTAB-10734:Sample 2

characteristic: INSDC center name

text: public

text: E-MTAB-10734:Sample 2

characteristic: age

text: ArrayExpress

text: bone marrow macrophage

characteristic: cell type

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: immunophenotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: male

text: Sample 2


E-MTAB-10734:Sample 4

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10734:Sample 4

text: ArrayExpress

text: bone marrow macrophage

text: house mouse

text: adult

text: Clec4a2 -/-

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: Sample 4


E-MTAB-10734:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10734:Sample 6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bone marrow macrophage

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Clec4a2 -/-

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: male

text: Sample 6


text: USDA-ARS

characteristic: INSDC center name

text: public

unit: day

characteristic: age

text: ArrayExpress

text: maize

characteristic: cultivar

text: Protocols: For each biosample, total RNA was isolated from cryo‐ground tissue by buffered thiocyanate salts and phenol‐chloroform extractions as previously described in 10.1371/journal.pgen.1002208. All samples were grown in the field in clay-loam soil. Sample genotypes were verified, and tissue organs collected on the dates indicated. mRNA enrichment and purification was performed by BGI using Oligo dT Selection to enrich the mRNA. Oligo dT Library construction was performed by BGI with the BGISEQ NGS platform.

text: early R3

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: embryo

text: Zea mays subsp. mays

characteristic: title


characteristic: ENA-CHECKLIST

text: USDA-ARS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: maize

text: early R3

text: wild type genotype

text: Zea mays subsp. mays

characteristic: organism

text: Zea mays subsp. mays

text: endosperm

characteristic: organism part

text: Zea mays subsp. mays


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-ARS

text: public

characteristic: SRA accession

text: ArrayExpress

text: maize

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: immature ear

text: Zea mays subsp. mays


text: USDA-ARS

text: public

text: E-MTAB-10741:B104_immature_ear_rep2_first

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: maize

characteristic: common name

characteristic: description

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: immature ear

text: Zea mays subsp. mays


text: USDA-ARS

characteristic: INSDC center name

text: public

text: E-MTAB-10741:B104_immature_ear_rep2_second

characteristic: age

text: ArrayExpress

text: maize

characteristic: cultivar

characteristic: developmental stage

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: immature ear

text: Zea mays subsp. mays


characteristic: ENA-CHECKLIST

text: USDA-ARS

characteristic: INSDC first public

text: public

text: ArrayExpress

text: maize

text: wild type genotype

characteristic: genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: immature tassel

text: Zea mays subsp. mays

characteristic: title


text: USDA-ARS

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: maize

text: late R1

text: wild type genotype

text: Zea mays subsp. mays

characteristic: organism

text: Zea mays subsp. mays

text: lower tassel

characteristic: organism part

text: Zea mays subsp. mays


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-ARS

text: public

characteristic: SRA accession

text: ArrayExpress

text: maize

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: leaf base

text: Zea mays subsp. mays


text: USDA-ARS

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: maize

characteristic: common name

characteristic: description

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: leaf middle

text: Zea mays subsp. mays


text: USDA-ARS

characteristic: INSDC center name

text: public

characteristic: age

text: ArrayExpress

text: maize

characteristic: cultivar

characteristic: developmental stage

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: leaf tip

text: Zea mays subsp. mays


characteristic: ENA-CHECKLIST

text: USDA-ARS

characteristic: INSDC first public

text: public

text: ArrayExpress

text: maize

text: 7 DAS

text: wild type genotype

characteristic: genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: shoot

text: Zea mays subsp. mays

characteristic: title


text: USDA-ARS

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: maize

text: 7 DAS

text: wild type genotype

text: Zea mays subsp. mays

characteristic: organism

text: Zea mays subsp. mays

text: root

characteristic: organism part

text: Zea mays subsp. mays


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: USDA-ARS

text: public

characteristic: SRA accession

text: ArrayExpress

text: maize

text: wild type genotype

text: Zea mays subsp. mays

text: Zea mays subsp. mays

text: immature ear (mid-section)

text: Zea mays subsp. mays


E-MTAB-10743:Sample 1

text: public

text: E-MTAB-10743:Sample 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: myeloid cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Single cells from aortas were digested in 5% FBS/RPMI containing 450 U/ml collagenase I, 125 U/ml collagenase XI 60 U/ml hyaluronidase and 60 U/ml DNase I for 50 minutes at 37°C. Cells were retrieved by passing tissue pieces through a 70 um cell strainer and were pooled from 9 mice in each experiment. Unspecific binding was blocked by incubating cells with murine anti-CD16/32 antibody. Cells were stained with antibodies against CD45, CD11b and CD64 and LIVE/DEAD viability dye. Live CD45+ CD11b+ (two experiments) or live CD45+ CD11b+ CD64low-high (one experiment) cells were sorted for sequencing. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3ʹ Reagents Kits (User Guide: CG00052). Quantification of cDNA and library construction was performed using a Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and high-sensitivity DNA tapestation (Agilent).

characteristic: description

text: adult

text: high fat diet

characteristic: diet

text: atherosclerosis

characteristic: disease

text: Apolipoprotein E knockout

text: high fat diet for 12 weeks

characteristic: growth condition

characteristic: immunophenotype

text: pool of 9 mice

characteristic: individual

text: Mus musculus

text: Mus musculus

text: aorta

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 1