characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Maarten Christenhusz

characteristic: collected by

text: Independent

text: United Kingdom

characteristic: geographic location (elevation)

text: SURREY | KEW GARDENS

text: cultivated from wild source

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Verbascum nigrum

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Verbascum nigrum

text: HERMAPHRODITE MONOECIOUS

text: 11a6bc91-d4ac-49ac-8e4e-2d5d686cd7dc-DTOL-specimen

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Maarten Christenhusz

text: Independent

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

unit: m

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SURREY | KEW GARDENS

characteristic: geographic location (region and locality)

text: woodland

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Agrimonia eupatoria

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Agrimonia eupatoria

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

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characteristic: tolid

type: derived from


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Maarten Christenhusz

characteristic: collected by

text: Independent

text: United Kingdom

characteristic: geographic location (elevation)

text: SURREY | KEW GARDENS

text: lawn

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Achillea millefolium

characteristic: organism

text: WHOLE ORGANISM

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text: HERMAPHRODITE MONOECIOUS

text: 83279de7-99bc-4fdc-8118-6aa740ffe1dc-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Maarten Christenhusz

text: Independent

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SURREY | KEW GARDENS

characteristic: geographic location (region and locality)

text: lawn

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Lolium perenne

text: WHOLE ORGANISM

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text: DTOL

characteristic: project name

text: Lolium perenne

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

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characteristic: tolid


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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characteristic: barcoding center

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text: Douglas-fir

characteristic: common name

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text: garden

text: Maarten Christenhusz

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text: vegetative structure

text: Pseudotsuga menziesii

characteristic: organism

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text: HERMAPHRODITE MONOECIOUS

text: a740c566-b43f-4a0c-b472-dac8c58812e4-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Maarten Christenhusz

text: Independent

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: SURREY | KEW GARDENS

characteristic: geographic location (region and locality)

text: cultivated from wild source

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text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Hedera hibernica

text: WHOLE ORGANISM

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text: DTOL

characteristic: project name

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text: HERMAPHRODITE MONOECIOUS

characteristic: sex

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characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Maarten Christenhusz | Sahr Mian | Ilia Leitch | Andrew Leitch

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: WEST SUSSEX | SHOREHAM | WIDEWATER LAGOON

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Frankenia laevis

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Frankenia laevis

text: HERMAPHRODITE MONOECIOUS

text: fe6c9f06-3b2f-4cc5-a6d2-70813b645ea0-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Puccinellia maritima

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Puccinellia maritima

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

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characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Beta vulgaris

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Beta vulgaris

text: HERMAPHRODITE MONOECIOUS

text: 1b7352ff-a1eb-4228-98a6-7da693e24da1-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Tamarix gallica

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Tamarix gallica

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

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text: b1e50cc5-7020-4a70-a7de-c13adfd7037e-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Salicornia ramosissima

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Salicornia ramosissima

text: HERMAPHRODITE MONOECIOUS

text: d38456a4-303d-4c13-896d-38d75f7f1be3-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Salicornia ramosissima

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Salicornia ramosissima

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

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characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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characteristic: barcoding center

text: COPO

characteristic: broker name

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Salicornia ramosissima

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Salicornia ramosissima

text: HERMAPHRODITE MONOECIOUS

text: 1949c9d9-4ef6-49b3-8578-1ba73e501da2-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Salicornia ramosissima

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Salicornia ramosissima

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

characteristic: specimen voucher

text: bc43a3c6-7dc0-49c7-b533-1376fc6f4957-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: EAST SUSSEX | CUCKMERE HAVEN

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Artemisia maritima

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Artemisia maritima

text: HERMAPHRODITE MONOECIOUS

text: 3315d359-9567-447b-b7ee-4cabe279edc6-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: EAST SUSSEX | CUCKMERE HAVEN

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Halimione portulacoides

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Halimione portulacoides

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

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text: e647158f-8736-4fd9-80f9-12af997b5305-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

characteristic: geographic location (elevation)

text: EAST SUSSEX | CUCKMERE HAVEN

text: saltmarsh

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Ruppia maritima

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Ruppia maritima

text: HERMAPHRODITE MONOECIOUS

text: e7297bc5-12b2-47c3-85ef-89601c0d63b5-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Royal Botanic Gardens Kew

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: ROYAL BOTANIC GARDEN EDINBURGH

text: COPO

text: Royal Botanic Gardens, Kew

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: EAST SUSSEX | CUCKMERE HAVEN

characteristic: geographic location (region and locality)

text: saltmarsh

characteristic: habitat

text: Maarten Christenhusz

characteristic: identified by

text: Independent

text: vegetative structure

characteristic: lifestage

text: Trifolium fragiferum

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Trifolium fragiferum

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

characteristic: specimen id

characteristic: specimen voucher

text: 8be59d41-6723-435c-9383-e2ef366aa443-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Gardens Kew

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ROYAL BOTANIC GARDEN EDINBURGH

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Sahr Mian

characteristic: collected by

text: Royal Botanic Gardens, Kew

text: United Kingdom

text: SURREY | KEW GARDENS

text: cultivated from wild source

text: Maarten Christenhusz

text: Independent

text: vegetative structure

text: Atriplex glabriuscula

characteristic: organism

text: WHOLE ORGANISM

text: DTOL

text: Atriplex glabriuscula

text: HERMAPHRODITE MONOECIOUS

text: b87a8856-22e8-470e-9535-1a839ee3f585-DTOL-specimen

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Systems Biology and Bioinformatics, Justus Liebig University Giessen

text: ArrayExpress

text: Protocols: Exponentially growing M. xanthus in CTT were harvested at 5,000 g for 5 min and resuspended in MC7 buffer (10 mM MOPS pH 6.8, 1 mM CaCl2) to 7×109 cells ml-1. 1 ml of concentrated cells was added to 10 ml of MC7 buffer in a polystyrene Petri dish with a diameter of 9.2 cm (Sarstedt). Total RNA from M. xanthus cells developed under submerged conditions was extracted from cells using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s protocol. Purified RNA was treated with TURBO DNA-free™ Kit (Invitrogen) according to the manufacturer’s protocol. RNA integrity was analyzed by 1% agarose gel electrophoresis. ' triphosphorylated RNA was capped with 3'-desthiobiotin-TEG-guanosine 5' triphosphate (DTBGTP) (New England Biolabs) using the vaccinia capping enzyme (VCE) (New England Biolabs). Then biotinylated RNA molecules were captured using streptavidin beads and eluted with a biotin-containing buffer. RNA samples were poly(A)-tailed using poly(A) polymerase. Then the 5'-PPP or CAP structures were converted to 5’-P using CAP-Clip Acid Pyrophosphatase (Cellscript). Afterwards, an RNA adapter was ligated to the newly formed 5'-monophosphate structures. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and M-MLV reverse transcriptase. The resulting cDNAs were PCR-amplified using a proof-reading enzyme. The libraries were amplified in 15 cycles of PCR. The generated cDNA libraries were sequenced on an Illumina NextSeq 500 system using 75 bp read length.

characteristic: description

text: vegetative state

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: MC7 submerged condition

characteristic: growth condition

text: Myxococcus xanthus

text: Myxococcus xanthus

text: whole organism

characteristic: organism part

text: Myxococcus xanthus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Systems Biology and Bioinformatics, Justus Liebig University Giessen

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: vegetative state

text: wild type genotype

text: MC7 submerged condition

text: Myxococcus xanthus

text: Myxococcus xanthus

text: whole organism

text: Myxococcus xanthus


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Exponentially growing M. xanthus in CTT were harvested at 5,000 g for 5 min and resuspended in MC7 buffer (10 mM MOPS pH 6.8, 1 mM CaCl2) to 7×109 cells ml-1. 1 ml of concentrated cells was added to 10 ml of MC7 buffer in a polystyrene Petri dish with a diameter of 9.2 cm (Sarstedt). Total RNA from M. xanthus cells developed under submerged conditions was extracted from cells using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s protocol. Purified RNA was treated with TURBO DNA-free™ Kit (Invitrogen) according to the manufacturer’s protocol. RNA integrity was analyzed by 1% agarose gel electrophoresis. rRNA depletion was conducted with 1 µg total RNA using Ribo-Zero rRNA Removal Kit Bacteria (Illumina), followed by library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Library preparation included 11 cycles of PCR amplification. Quality and quantity were assessed at all steps via capillary electrophoresis (TapeStation, Agilent Technologies) and fluorometry (Qubit, Thermo Fisher Scientific).

text: vegetative cells

text: wild type genotype

text: cells developed under mc7 submerged conditions

text: Myxococcus xanthus

text: Myxococcus xanthus

characteristic: replicate

text: Myxococcus xanthus


characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

characteristic: broker name

text: developing cells

text: wild type genotype

text: Myxococcus xanthus

characteristic: organism

text: Myxococcus xanthus

text: Myxococcus xanthus

characteristic: title


text: NDORMS, University of Oxford

text: public

tag: Namespace:NDORMS, University of Oxford

unit: year

characteristic: age

text: ArrayExpress

text: mononuclear cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Blood and synovial fluid samples were collected with written informed patient consent from AS patients undergoing intra-articular aspiration at Oxford University Hospitals. The study was performed in accordance with protocols approved by the Oxford Research Ethics committee (Ethics reference number 06/Q1606/139). Mononuclear cells were immediately isolated by density centrifugation then stained by a panel of fluorescently conjugated antibodies in staining buffer (RNAse-free PBS, 2mM EDTA). AS samples were stained with the following antibodies: CD3-PerCP-Cy5.5 (OKT3), CD8a-PE (RPA-T4), CD45RA-PE/Dazzle (HI100), CD25-PE (BC96), CD127-PE/Cy7 (A019D5) (all from Biolegend, and used at 1:50 dilution) and Fixable Viability Dye eFluor520 (eBioscience, dilution 1:250) to exclude dead cells. Cells were sorted on a Sony SH800Z. Memory Tregs were sorted as CD45RA-(negative) CD3+ CD25+ CD127low. Simultaneously memory CD4 (CD45RA-CD3+CD4+CD8-), memory CD8 (CD45RA-CD3+CD8+CD4-) and CD3- fractions were also sorted. The sample was combined in a 1:1:1:0,5 ratio before loading onto the 10x Chromium Controller. Cells were then collected in a collection buffer (Phenol Red-ve RPMI + 4% Bovine Serum Albumin + Hepes 25mM). After sorting, cells were stained separately with Fc blocker (concentration 1:20) (TruStain FcX, Biolegend), rested for 15 minutes, and then resuspended in buffer to be stained with assigned oligonucleotide-tagged antibodies (concentration 1:200) (TotalSeq C, Biolegend). Cells were washed two more times, then resuspended in FACS collection buffer and kept cold until use.. Cells were again washed twice with FACS buffer then kept on ice until loaded onto the Chromium controller. For sample AS02, PBMC and SFMC were not processed fresh but thawed after being cryopreserved in liquid nitrogen. A separate channel was used for the synovial and blood derived cells from each patient. Standard 10x protocols for the kit were used to load cells into droplets containing barcodes. Cells were captured and cDNA preparation performed according to the Single Cell 5′ Protocol recommended by 10x Genomics.

characteristic: description

text: adult

characteristic: developmental stage

text: ankylosing spondylitis

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NDORMS, University of Oxford

characteristic: INSDC center name

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text: Protocols: Corynebacterium glutamicum strain AS019E12 was used as the wild type strain. The plasmid expressing Myc-OsnR was constructed as follows: Primers carrying the sequences needed for the amplification of the osnR gene were designed and additional sequences, which can optimally express the Myc epitope in C.glutamicum. All C. glutamicum strains were cultured in 100 ml MCGC medium at 30 °C to the final OD600 of ~2.0. Bacteria cells were treated with formaldehyde (final concentration 1%) for 20 min at 30°C. Formaldehyde was added to cultures at a final concentration of 1% (v/v), and incubation was continued for 20 min. Subsequently, glycine was added to the final concentration of 125 mM, followed by incubation at room temperature for 5 min. The cells were harvested via centrifugation at 1,600 g for 10 min and washed twice with ice-cold phosphate-buffered saline (pH 7.4). They were then resuspended in 0.5 ml lysis buffer composed of 1% sodium dodecyl sulfate, 1% Triton X-100, 10 mM EDTA, 50 mM Tris-HCl (pH 8.1), 1 mM PMSF, and 5 μg/ml RNase A, incubated at 30 °C for 10 min, and then chilled on ice. The lysate was subjected to sonication 10 times for 20 s by using sonicator (microprobe diameter 3 mm; Sonics & Materials, Inc.) at 30% amplitude and each resting on ice for 30 s to obtain chromosomal DNA fragments of 200-500 bp. Cell debris was removed via centrifugation at 11,000 g for 10 min. Crosslinked bacteria cells were sonicated and protein-DNA complexes were isolated with an c-Myc monoclonal antibody(9E10). Libraries were prepared according to Illumina's instructions. For total DNA extraction, the immunoprecipitated and control input DNA samples were diluted with the elution buffer and then treated with 20 μl proteinase K solution for 2 h at 65 °C. The samples were then purified using DNA clean-up columns (Thermo Fisher Scientific), precipitated, and resuspended in water.

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