characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Children’s Brain Tumour Research Centre, School of Medicine; Biodiscovery Institute, University of Nottingham, Nottingham, UK

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ArrayExpress

text: Daoy

text: human

text: medulloblastoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: Homo sapiens

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Children’s Brain Tumour Research Centre, School of Medicine; Biodiscovery Institute, University of Nottingham, Nottingham, UK

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: common name

text: Protocols: Cell pellets of at least 100,000 cells were washed with HBSS and frozen in liquid nitrogen. Medulloblastoma cell lines representating the different subgroups have been cultured and cell were harvested and RNA was isolated when 70% confluency was reached. In detail, DAOY and D458 were grown in DMEM (Thermo Fisher) with 10% FBS (HyClone, Thermo Fisher), ONS-76 and HD-MB03 were grown in RPMI 1640 (Sigma-Aldrich) with 10% FBS and CHLA-01-MED and CHLA-01R-MED were grown in DMEMF12 supplemented with B27, 20 ng/ml EGF and 20 ng/ml bFGF (all Thermo Fisher). All cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2. During the course of this study, all cell lines were routinely confirmed to be mycoplasma negative (MycoAlert, Lonza, Basel, Switzerland). For homogenization, ceramic spheres (Lysing Matrix D, MP Biomedicals, Santa Ana, California, USA) and the FastPrep-24 homogenizer was used (MP Biomedicals, speed 4 m/s, tube holder MP:24*2 and time 20 s). Total RNA was isolated from 2D pellets using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions. In total, 3 biological replicates of each cell line were processed respectively. RNA amount was determined using the Qubit RNA BR kit with the Qubit 4 (both ThermoFisher). Library preparation/sequencing/analysis were performed by Novogene (UK) Company Limited. RNA sample was used for library preparation. Ribosomal RNA was firstly removed. Then the directional sequencing library was constructed using NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s protocol. Indices were included to multiplex multiple samples. Briefly, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The strand-specific library was ready after end repair, A-tailing, adapter ligation, size selection and USER enzyme digestion. After amplification and purification, insert size of the library was validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR).

characteristic: description

text: medulloblastoma

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

characteristic: replicate

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:UNIVERSITY OF COPENHAGEN (SECTION OF ORGANISMAL BIOLOGY)

text: J.-O. Skage

characteristic: collected by

characteristic: collection date

text: Axenic mono-spore isolate of plant pathogenic fungi

text: Norway

characteristic: geographic location (country and/or sea)

text: Fana

characteristic: geographic location (region and locality)

text: Abies lasiocarpa

characteristic: host scientific name

text: NO 252125

characteristic: isolate

text: 60.26, 5.34

characteristic: lat lon

text: Neonectria neomacrospora

text: Neonectria neomacrospora

text: Nneo genomics


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF MELBOURNE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF MELBOURNE

characteristic: Submitter Id

text: E. coli

text: USA

text: In vitro culture

characteristic: isolation source

text: Escherichia coli

characteristic: organism

text: Escherichia coli

characteristic: title


ECN

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF MELBOURNE

characteristic: INSDC center name

text: public

text: ECN

text: E. coli

characteristic: common name

text: USA

text: In vitro culture

text: Escherichia coli

text: Escherichia coli

text: ECN


EHEC

text: UNIVERSITY OF MELBOURNE

text: public

text: EHEC

characteristic: collection date

text: E. coli

text: USA

characteristic: geographic location (country and/or sea)

text: In vitro culture

text: Escherichia coli

text: Escherichia coli

text: EHEC


text: Institute for Cereak Crops Improvement

text: public

tag: Namespace:Institute for Cereak Crops Improvement

characteristic: cultivar

text: Aegilops longissima accession 6782 library:PE450

characteristic: description

text: AEG67822_1_DDP03349_HGN2TBCX2_L1_1.fq.gz

characteristic: filename

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf

characteristic: tissue type

text: Aegilops longissima accession 6782 WGS sequences


characteristic: ENA-LAST-UPDATE

text: Institute for Cereak Crops Improvement

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: AEG67822_1_DDP03349_HGN2TBCX2_L1_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: AEG67822_1_DDP03349_HKCYWBCX2_L1_1.fq.gz

text: Aegilops longissima

characteristic: organism

text: Aegilops longissima

text: Novogene

text: leaf


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03349_HKCYWBCX2_L1_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf

characteristic: title


characteristic: External Id

text: Institute for Cereak Crops Improvement

characteristic: INSDC center name

text: public

text: AEG67822_1_DDP03349_HKCYWBCX2_L2_1.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03349_HKCYWBCX2_L2_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03349_hkgjgbcx2_L2_1.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03349_hkgjgbcx2_L2_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03350_HGN2TBCX2_L2_1.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03350_HGN2TBCX2_L2_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03350_HM2GTBCX2_L1_1.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


text: Institute for Cereak Crops Improvement

text: public

text: AEG67822_1_DDP03350_HM2GTBCX2_L1_2.fq.gz

text: Aegilops longissima

text: Aegilops longissima

text: Novogene

text: leaf


E-MTAB-9835:Sample 1

text: public

text: E-MTAB-9835:Sample 1

tag: Namespace:Laboratory of Molecular Medicine and Genomics

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: common name

text: Protocols: Human PCa cell lines, PC3 (RRID:CVCL_0035) were obtained from American type culture collection (ATCC, Manassas, VA, USA) and regularly tested for mycoplasma (N-GARDE Mycoplasma PCR Reagent set, EuroClone, Milan, IT). Both cell lines were cultured in RPMI 1640 (EuroClone) supplemented with 10% heat-inactivated foetal bovine serum (EuroClone), 2 mM glutammine and 100 U/ml penicillin/ streptomycin (EuroClone). Total RNA was isolated from cells with TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA, USA). For mRNA quantification, complementary DNAs were prepared retro-transcribing 1 μg of total RNA with iScript Reverse Transcription supermix for reverse transcriptase-quantitative PCR (Bio-Rad, Hercules, CA, USA). Quantitative reverse transcriptase-PCR reactions were performed with iQ SYBR Green supermix (Bio-Rad). The PCR was monitored in real-time using a MJ-Chromo4 (Bio-Rad) that allowed determination of the threshold cycle (Ct) at the beginning of the exponential amplification of the PCR products. The average Ct, from triplicate assays, was used for further calculations. For small RNA sequencing, 1 ug of total RNA was used for indexed sequencing library preparation with a TruSeq small RNA Sample Prep Kit (Illumina, San Diego, CA, USA) and sequenced on HiSeq1500 (Illumina) for 50 cycles

text: prostate cancer

characteristic: disease

text: cancer-associated fibroblast conditioned medium for 3 days

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: prostate

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: Sample 1


E-MTAB-9835:Sample 2

characteristic: ENA-LAST-UPDATE

text: Laboratory of Molecular Medicine and Genomics

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-9835:Sample 2

text: ArrayExpress

text: human

characteristic: description

text: prostate cancer

text: Homo sapiens

text: Homo sapiens

text: prostate

text: Homo sapiens

text: Sample 2


E-MTAB-9835:Sample 3

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9835:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: human

text: prostate cancer

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: prostate

text: Homo sapiens

text: Sample 3


E-MTAB-9835:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: public

text: E-MTAB-9835:Sample 7

text: ArrayExpress

text: human

text: prostate cancer

text: benign hyperplastic fibroblast conditioned medium for 3 days

text: Homo sapiens

text: Homo sapiens

text: prostate

text: Homo sapiens

text: Sample 7

characteristic: title


E-MTAB-9835:Sample 8

characteristic: INSDC center name

text: public

text: E-MTAB-9835:Sample 8

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: common name

text: prostate cancer

text: Homo sapiens

text: Homo sapiens

text: prostate

text: Homo sapiens

text: Sample 8


E-MTAB-9835:Sample 9

text: public

text: E-MTAB-9835:Sample 9

text: ArrayExpress

text: human

text: prostate cancer

characteristic: disease

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: prostate

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: Sample 9


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Fraunhofer Attract Eco'n'OMICs, Fraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, Germany  Evolutionary Ecology and Environmental Toxicology, Faculty Biological Sciences, Goethe University Frankfurt, Frankfurt, Germany

unit: hour

characteristic: age

characteristic: batch

text: ArrayExpress

text: zebrafish

text: Protocols: Catalogue no. 2921-88-2. For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Chlorpyrifos (CAS 2921-88-2, PESTANAL® analytical standard) was purchased from Merck KGgA (Darmstadt, Germany). For the test solution preparation, first a high concentrated stock solution was prepared in Acetone (99.9% MS grade). From this stock, respective volumes for each testing concentration were added to a 250 ml DURAN glass bottle and filled up with pure Acetone to a final volume of 1 ml. To the bottle for the control solution only 1ml of pure Acetone was added. The solvent was then evaporated at room temperature until no Acetone was left in the bottles. Then 200 ml of Cu reduced water were added and solutions were stirred in the dark for 3 hours at room temperature. The solutions were freshly prepared one day prior the start of the experiment and stored at room temperature in the dark. They were constantly aerated and used over the course of the 96 hours exposure experiment. The glass wells for the incubation of the embryos were pre-saturated with the respective test solution overnight and renewed at the beginning of the experiment. Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

characteristic: description

text: larval day 4

characteristic: developmental stage

characteristic: extraction date

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: rin

text: Danio rerio

text: mixed

characteristic: sex

text: AB

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Fraunhofer Attract Eco'n'OMICs, Fraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, Germany Evolutionary Ecology and Environmental Toxicology, Faculty Biological Sciences, Goethe University Frankfurt, Frankfurt, Germany

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: zebrafish

characteristic: common name

text: larval day 4

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

text: Danio rerio

text: mixed

text: AB

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

characteristic: batch

text: ArrayExpress

text: zebrafish

text: Protocols: For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. CASRN: 71751-41-2 Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Abamectin (CAS: 71751-41-2, PESTANAL© analytical grade) was purchased from Merck KGgA (Darmstadt, Germany). For the test solution preparation, first a high concentrated stock solution was prepared in Acetone (99.9% MS grade). From this stock, respective volumes for each testing concentration were added to a 250 ml DURAN glass bottle and filled up with pure Acetone to a final volume of 1 ml. To the bottle for the control solution only 1ml of pure Acetone was added. The solvent was then evaporated at room temperature until no Acetone was left in the bottles. Then 200 ml of Cu reduced water were added and solutions were stirred in the dark for 3 hours at room temperature. The solutions were freshly prepared one day prior the start of the experiment and stored at room temperature in the dark. They were constantly aerated and used over the course of the 96 hours exposure experiment. The glass wells for the incubation of the embryos were pre-saturated with the respective test solution overnight and renewed at the beginning of the experiment. Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

characteristic: description

text: larval day 4

characteristic: developmental stage

text: wild type genotype

text: mixed pool of 10 larvae

characteristic: individual

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

text: Danio rerio

text: mixed

text: AB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: cas

text: zebrafish

text: control

characteristic: condition

text: Protocols: For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Fipronil (CAS 120068-37-3, TraceCERT®) was purchased from Merck KGgA (Darmstadt, Germany). For the test solution preparation, first a high concentrated stock solution was prepared in Acetone (99.9% MS grade). From this stock, respective volumes for each testing concentration were added to a 250 ml DURAN glass bottle and filled up with pure Acetone to a final volume of 1 ml. To the bottle for the control solution only 1ml of pure Acetone was added. The solvent was then evaporated at room temperature until no Acetone was left in the bottles. At last, 200 ml of Cu reduced water were added and solutions were stirred in the dark for 3 hours at room temperature. The test solutions were freshly prepared one day prior the start of the experiment and stored at room temperature in the dark. They were constantly aerated and used over the course of the 96 hours exposure experiment. The glass wells for the incubation of the embryos were pre-saturated with the respective test solution overnight and renewed at the beginning of the experiment. Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

text: larval stage

characteristic: extraction date

text: wild type genotype

characteristic: genotype

unit: gram per liter

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: pooled individuals

characteristic: rin

text: Danio rerio

text: mixed

characteristic: sex

text: AB

characteristic: strain

characteristic: tank spawning group


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: zebrafish

characteristic: common name

text: high exposure

characteristic: description

text: larval stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

text: Danio rerio

text: mixed

text: AB

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: cas

text: zebrafish

text: low exposure

characteristic: condition

text: larval stage

characteristic: developmental stage

characteristic: extraction date

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

characteristic: pooled individuals

text: Danio rerio

text: mixed

text: AB


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: zebrafish

text: control

text: Protocols: For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Carbaryl (CAS 63-25-2, PESTANAL®) was purchased from Merck KGgA (Darmstadt, Germany). For the test solution preparation, first a high concentrated stock solution was prepared in Acetone (99.9% MS grade). From this stock, respective volumes for each testing concentration were added to a 250 ml DURAN glass bottle and filled up with pure Acetone to a final volume of 1 ml. To the bottle for the control solution only 1ml of pure Acetone was added. The solvent was then evaporated at room temperature until no Acetone was left in the bottles. At last, 200 ml of Cu reduced water were added and solutions were stirred in the dark for 3 hours at room temperature.The test solutions were freshly prepared one day prior the start of the experiment and stored at room temperature in the dark. They were constantly aerated and used over the course of the 96 hours exposure experiment. The glass wells for the incubation of the embryos were pre-saturated with the respective test solution overnight and renewed at the beginning of the experiment.Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

text: larval day 4

text: wild type genotype

characteristic: nominal conc

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: rin

text: Danio rerio

text: mixed

characteristic: sex

text: AB

characteristic: strain

characteristic: tank spawning group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cas

text: zebrafish

characteristic: common name

text: high exposure

characteristic: condition

characteristic: description

text: larval day 4

characteristic: developmental stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

text: Danio rerio

text: mixed

text: AB

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: zebrafish

text: low exposure

text: larval day 4

text: wild type genotype

characteristic: genotype

characteristic: nominal conc

text: Danio rerio

text: Danio rerio

text: whole organism

characteristic: organism part

characteristic: pooled individuals

text: Danio rerio

text: mixed

text: AB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

characteristic: cas

text: zebrafish

characteristic: common name

text: Control

text: Protocols: For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Imidacloprid (CAS 138261-41-3, PESTANAL® analytical standard, purity ≥ 98.0 %) was purchased from Merck KGgA (Darmstadt, Germany). Test solutions were freshly prepared one day prior starting the exposure experiment. For that the solutions were setup in 200 ml of Cu reduced water and stirred in the dark for 3 hours at room temperature before adding 8 ml of the respective test solution to the glass wells for pre-saturation overnight. The remaining test solutions were stored in the dark at room temperature and the solution in the well was renewed the next day with the beginning of the experiment. Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

text: larval day 4

characteristic: extraction date

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: whole larvae

unit: count unit

characteristic: rin

text: Danio rerio

text: mal & femal (pooled)

characteristic: sex

text: AB

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

text: LowExposure

characteristic: condition

characteristic: description

text: larval day 4

characteristic: developmental stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole larvae

text: Danio rerio

text: mal & femal (pooled)

text: AB

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cas

text: zebrafish

text: MidExposure

text: larval day 4

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole larvae

characteristic: organism part

text: Danio rerio

text: mal & femal (pooled)

text: AB


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: zebrafish

characteristic: common name

text: HighExposure

text: larval day 4

characteristic: extraction date

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: whole larvae

characteristic: rin

text: Danio rerio

text: mal & femal (pooled)

characteristic: sex

text: AB

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: zebrafish

text: Control

characteristic: condition

text: Protocols: For each sample, 10 4-day old zebrafish larvae were randomly picked from the glass well and carefully transferred to a 1.5 ml low binding Eppendorf tube. Once individuals were collected for all samples, they were euthanized at the same time by placing the tubes simultaneously on ice for 10 minutes. Supernatant from the test solutions was then carefully removed without injuring the larvae before adding 350µl of RP1 buffer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250) for tissue homogenization. RP1 buffer was freshly prepared with TCEP as reducing agent prior extraction. For detailed information about embryonic incubation conditions and husbandry of adult broodstock please refer to the “Growth protocol”. For detailed information about exposure duration and conditions please refer to the “Treatment protocol”. Adult animal husbandry: Wild-type zebrafish (Danio rerio, Strain AB) broodstocks were maintained under flow-through conditions in 150 L tanks at 26 +/- 2°C on a 12:12 h light/dark cycle. They were fed daily with TetraMin® (Tetra Werke, Melle, Germany) main feed ad libitum and nauplii of Artemia salina. The day before test start, glass spawning-trays with artificial substrate (green glass beads stringed on stainless steel wire) were placed at the bottom of each tank. After mating and spawning in the morning hours eggs were rinsed with clear Cu-reduced water and placed into glass dishes for pre-picking. Fish embryo incubation: For each sample 15 fertilized eggs in the early blastula stage were placed in glass petri dishes (diameter 6 cm) filled with 8 ml of the respective testing solution (Control, Low exposure, High exposure). Embryos were incubated at 27 +/- 1°C on a 14:10 h light/dark cycle. At 24 hours post fertilization (hpf), eggs were inspected visually and single coagulated eggs were recorded and removed. At 48 hpf, overall survival, hatching rates, morphological malformations and physiological changes were recorded and aged solutions were replaced by fresh, aerated test solutions. At 96 hpf again, overall survival, hatching rates, morphological malformations and physiological changes were recorded before RNA and protein extraction. Methoxychlor (CAS 72-43-5, PESTANAL®) was purchased from Merck KGgA (Darmstadt, Germany). For the test solution preparation, first a high concentrated stock solution was prepared in Acetone (99.9% MS grade). From this stock, respective volumes for each testing concentration were added to a 250 ml DURAN glass bottle and filled up with pure Acetone to a final volume of 1 ml. To the bottle for the control solution only 1ml of pure Acetone was added. The solvent was then evaporated at room temperature until no Acetone was left in the bottles. At last, 200 ml of Cu reduced water were added and solutions were stirred in the dark for 3 hours at room temperature. The test solutions were freshly prepared one day prior the start of the experiment and stored at room temperature in the dark. They were constantly aerated and used over the course of the 96 hours exposure experiment. The glass wells for the incubation of the embryos were pre-saturated with the respective test solution overnight and renewed at the beginning of the experiment. Per sample, about 40 - 50 fertilized fish eggs were pre-picked in glass petri dishes (10 cm diameter) filled with 30 ml of the testing solution. Using an optical binokular microscope, 15 embryos of the same early blastula stage were transferred from the pre-picking plate into the test glass wells filled with 8 ml of the test solution. Embryos were then incubated as described in the “growth protocol”. The pooled larvae in RP1 buffer were transferred to a screw-cap eppendorf filled with 0.3 g of Lysing Matrix D (MP Biomedicals, 6913050) ceramic beads. Tissue homogenization was performed at 5 m/s for 45 s with FastPrep-24© (MP Biomedicals, 6004500) at room temperature. From here on total RNA and Protein was extracted from the tissue lysate using the NucleoSpin RNA Protein kit (Macherey-Nagel, 740933.250) according to the manufacturer’s protocol. RNA concentration (>100 ng/µl) was measured using a Nanodrop 2000 spectrophotometer (Thermo Scientific). The sample’s overall RNA quality and corresponding RIN value was fluorometrically determined with a 2100 Bioanalyzer© Instrument system (Agilent, G2939BA) using the Agilent RNA 6000 Nano Kit© (Agilent, 5067-1511) according to the manufacturer’s protocol. Only samples with a RIN > 8 were used for downstream analysis. Samples were stored at -80°C until they were send to the sequencing facility on dry ice. Sequencing libraries were prepared for each sample from 100 ng/µl total RNA at the sequencing facility “NGS-Services for Integrative Genomics” at the University of Göttingen in Germany. According to their standard workflow, cDNA libraries were prepared from protein coding mRNA that were purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing.

characteristic: description

text: larval day 4

characteristic: developmental stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole larvae

text: Danio rerio

text: male & female (pooled)

text: AB

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cas

text: zebrafish

text: LowExposure

text: larval day 4

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: whole larvae

characteristic: organism part

text: Danio rerio

text: male & female (pooled)

text: AB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: zebrafish

characteristic: common name

text: HighExposure

text: larval day 4

characteristic: extraction date

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: whole larvae

characteristic: rin

text: Danio rerio

text: male & female (pooled)

characteristic: sex

text: AB

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSERM

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: human

text: Protocols: RNA seq experiments were performed on RNA samples extracted from HMC1.2 cells transduced with pRRSLIN-GLI3R-tdTomato or pRRSLIN-control-tdTomato Nucleic acid extraction protocol :Total RNA was isolated from transducted HMC1.2 using the RNeasy Plus Minikit (Qiagen GmbH, Hilden, Germany), according to the manufacturer’s instructions. stranded polyA+ RNAseq librairies using Universal Plus mRNA-Seq kit (Nugen)

characteristic: description

text: mast-cell leukemia

characteristic: disease

text: pRRSLIN-control-tdTomato

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: human

text: mast-cell leukemia

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 34697:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

tag: Namespace:Leibniz Centre for Tropical Marine Research

text: GFBIO

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 6 months (sampled in January 2018) at the marina in Al Mouj (Oman)

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

text: FWD: CCTACGGGNGGCWGCAG; REV: GACTACNVGGGTATCTAATCC

characteristic: pcr primers

text: Biotechnological Applications of Marine Biofilms developing on solid surfaces in the Arabian Gulf

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz Centre for Tropical Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34696:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34695:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34694:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 5 months (sampled in December 2017) at the marina in Al Mouj (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34693:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34692:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34691:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 4 months (sampled in November 2017) at the marina in Al Mouj (Oman)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34690:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34689:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34688:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 3 months (sampled in October 2017) at the marina in Al Mouj (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34687:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34686:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34685:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 2 months (sampled in September 2017) at the marina in Al Mouj (Oman)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

text: FWD: CCTACGGGNGGCWGCAG; REV: GACTACNVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz Centre for Tropical Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34684:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: Biotechnological Applications of Marine Biofilms developing on solid surfaces in the Arabian Gulf

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34683:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34682:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 1 month (sampled in August 2017) at the marina in Al Mouj (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34681:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Al Mouj

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34680:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Al Mouj

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34679:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 6 months (sampled in January 2018) at the marina in Bandar Rowdha (Oman)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34678:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34677:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34676:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 5 months (sampled in December 2017) at the marina in Bandar Rowdha (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34675:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

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characteristic: project name

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text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34674:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34673:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 4 months (sampled in November 2017) at the marina in Bandar Rowdha (Oman)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

text: FWD: CCTACGGGNGGCWGCAG; REV: GACTACNVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz Centre for Tropical Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34672:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: Biotechnological Applications of Marine Biofilms developing on solid surfaces in the Arabian Gulf

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34671:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

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text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34670:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 3 months (sampled in October 2017) at the marina in Bandar Rowdha (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34669:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

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characteristic: project name

characteristic: sample description

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text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34668:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34667:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 2 months (sampled in September 2017) at the marina in Bandar Rowdha (Oman)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34666:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34665:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34664:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 1 month (sampled in August 2017) at the marina in Bandar Rowdha (Oman)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34663:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Oman

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Bandar Rowdha

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34662:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Oman

text: Bandar Rowdha

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34661:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: Bacterial biofouling community on acrylic panels deployed for 6 months (sampled in January 2018) at the marina in Salmiya (Kuwait)

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Kuwait

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salmiya

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

text: FWD: CCTACGGGNGGCWGCAG; REV: GACTACNVGGGTATCTAATCC

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz Centre for Tropical Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34660:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Kuwait

text: Salmiya

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: Biotechnological Applications of Marine Biofilms developing on solid surfaces in the Arabian Gulf

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 34659:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

text: GFBIO

text: biofilm [ENVO:00002034]

text: coastal water body [ENVO:02000049]

text: biofilm material [ENVO:01000156]

text: microbial mat/biofilm

text: Kuwait

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Salmiya

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample description

text: biofilm metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to INSDC

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: total depth of water column


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 34658:1930e2b6-9c4c-4874-adb7-91f9497ac2b8

characteristic: Submitter Id

text: GFBIO

characteristic: broker name

characteristic: collection date

text: Bacterial biofouling community on acrylic panels deployed for 5 months (sampled in December 2017) at the marina in Salmiya (Kuwait)

characteristic: description

text: biofilm [ENVO:00002034]

characteristic: environment (biome)

text: coastal water body [ENVO:02000049]

characteristic: environment (feature)

text: biofilm material [ENVO:01000156]

characteristic: environment (material)

text: microbial mat/biofilm

characteristic: environmental package

text: Kuwait

text: Salmiya

text: mimarks-survey

text: microbial mat/biofilm

text: biofilm metagenome

characteristic: organism

text: biofilm metagenome

text: Illumina MiSeq

text: true

text: 16S rRNA

characteristic: title