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characteristic: collection date

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GIFT

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characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Pit tag ID

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text: Nile tilapia

characteristic: common name

text: Malaysia

text: WorldFish HQ, Penang

text: Oreochromis niloticus

characteristic: organism

text: Oreochromis niloticus

text: male

characteristic: sex

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tag: Namespace:CIBIO-INBIO RESEARCH CENTER IN BIODIVERSITY AND GENETIC RESOURCES

characteristic: collection date

text: wine grape

text: Airen

characteristic: cultivar

text: Airen (Sequencing data no. 1 of 2)

characteristic: description

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characteristic: dev stage

text: Portugal

characteristic: geographic location (country and/or sea)

text: UTAD collection, Portugal

characteristic: isolation source

text: Vitis vinifera

text: Vitis vinifera

text: Vitis vinifera subsp. vinifera

characteristic: sub species

text: Leaf


characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

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text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Tree of Life Programme

characteristic: Submitter Id

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characteristic: collecting institution

text: Anopheles coluzzii

text: Australia

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: East Anglia | Norfolk | Norwich

characteristic: geographic location (region and locality)

characteristic: habitat

text: HIENNGUYEN

text: INSTITUTE B

text: adult

characteristic: lifestage

text: Bifurcaria bifurcata

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Trio child of SPECIMEN ID1 and SPECIMEN ID3

characteristic: relationship

text: Bifurcaria bifurcata

text: FEMALE

text: aNOT PROVIDED

text: N

characteristic: symbiont

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: abc QUEANH

text: INSTITUTE A

characteristic: collection date

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characteristic: geographic location (country and/or sea)

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text: agarden

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text: Bugulina stolonifera

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: Bugulina stolonifera

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: QUE1 ANH

text: INSTITUTE A

characteristic: collecting institution

text: Anopheles coluzzii

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characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Norwich

characteristic: geographic location (region and locality)

text: garden

characteristic: habitat

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text: vegetative structure

characteristic: lifestage

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text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Bugula neritina

text: HERMAPHRODITE MONOECIOUS

text: NOT PROVIDED

text: N


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: QUEANH

text: INSTITUTE A

characteristic: collection date

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text: Australia

characteristic: geographic location (country and/or sea)

text: East Anglia | Norfolk | Norwich

text: garden

text: HIENNGUYEN

text: INSTITUTE B

text: adult

text: Bombus hypnorum

text: WHOLE ORGANISM

text: ToL

characteristic: relationship

text: Bombus hypnorum

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: QUEANH

text: INSTITUTE A

characteristic: collecting institution

text: Anopheles coluzzii

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characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Norwich

characteristic: geographic location (region and locality)

text: garden

characteristic: habitat

text: QUEANH

text: INSTITUTE A

text: adult

text: Blastobasis adustella

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: Blastobasis adustella

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

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characteristic: INSDC last update

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characteristic: collection date

text: Anopheles coluzzii

text: Australia

characteristic: geographic location (country and/or sea)

text: East Anglia | Norfolk | Norwich

text: HIENNGUYEN

text: INSTITUTE B

text: adult

characteristic: lifestage

text: Bifurcaria bifurcata

text: HEAD

characteristic: organism part

characteristic: project name

characteristic: sample derived from

text: Bifurcaria bifurcata

text: FEMALE

text: aNOT PROVIDED

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: abc QUEANH

text: INSTITUTE A

characteristic: collecting institution

text: Anopheles coluzzii

text: United Kingdom

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Norwich

characteristic: geographic location (region and locality)

text: agarden

characteristic: habitat

text: HIENNGUYEN

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text: adult

text: Bugulina stolonifera

characteristic: organism

text: THORAX

characteristic: relationship

text: Bugulina stolonifera

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

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text: Tree of Life Programme

characteristic: INSDC center name

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characteristic: INSDC last update

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characteristic: collection date

text: Anopheles coluzzii

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Norwich

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text: vegetative structure

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text: WHOLE ORGANISM

characteristic: organism part

characteristic: sample same as

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text: HERMAPHRODITE MONOECIOUS

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: title


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: QUEANH

text: INSTITUTE A

characteristic: collecting institution

text: Anopheles coluzzii

text: Australia

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: East Anglia | Norfolk | Norwich

characteristic: geographic location (region and locality)

text: garden

characteristic: habitat

text: HIENNGUYEN

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text: adult

text: Bombus hypnorum

characteristic: organism

text: CEPHALOTHORAX

characteristic: project name

characteristic: relationship

characteristic: sample derived from

text: Bombus hypnorum

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: QUEANH

text: INSTITUTE A

characteristic: collection date

text: Anopheles coluzzii

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Norwich

text: garden

text: QUEANH

text: INSTITUTE A

text: adult

characteristic: lifestage

text: Blastobasis adustella

text: ABDOMEN

characteristic: organism part

text: Blastobasis adustella

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

characteristic: collecting institution

text: lugworm

characteristic: common name

text: United Kingdom

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: DARK FOREST

characteristic: geographic location (region and locality)

text: Woodland

characteristic: habitat

text: JO IDENTIFIER

text: THE IDENTIFIER INSTITUTE

text: adult

text: Arenicola marina

characteristic: organism

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: dsfghgvdfksl

characteristic: relationship

text: Arenicola marina

text: FEMALE

text: N

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

characteristic: collection date

text: lugworm

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: DARK FOREST

text: Woodland

text: JO IDENTIFIER

text: THE IDENTIFIER INSTITUTE

text: adult

characteristic: lifestage

text: Arenicola marina

text: MUSCLE

characteristic: organism part

text: SuperProject

text: dsfghgvdfksl

characteristic: sample derived from

text: Arenicola marina

text: FEMALE

characteristic: sex

text: N

characteristic: symbiont


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: DARK FOREST

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: JINGCHUN LI | RUIQI LI

text: University of Colorado Boulder | University of Colorado Boulder

text: juvenile

text: Euphrasia confusa

characteristic: organism

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: Euphrasia confusa

text: FEMALE

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: DARK FOREST

text: NOT COLLECTED

text: JINGCHUN LI | RUIQI LI

text: juvenile

text: Bugulina stolonifera

text: WHOLE ORGANISM

text: ToL

text: Bugulina stolonifera

text: FEMALE

characteristic: title


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: DARK FOREST

text: NOT COLLECTED

text: JINGCHUN LI | RUIQI LI

text: juvenile

characteristic: lifestage

text: Euphrasia confusa

text: MUSCLE

characteristic: organism part

text: HIENNTT

characteristic: sample derived from

text: Euphrasia confusa

text: FEMALE

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

text: public

text: ALEX COLLECTOR

text: THE COLLECTOR INSTITUTE

text: United Kingdom

text: DARK FOREST

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: JINGCHUN LI | RUIQI LI

text: juvenile

text: Bugulina stolonifera

characteristic: organism

text: MUSCLE

text: HIENNTT

characteristic: project name

text: Bugulina stolonifera

text: FEMALE

characteristic: tolid


text: University of Antioquia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-25-11-2021-04:27:10:859-6844

tag: Namespace:University of Antioquia

characteristic: Submitter Id

characteristic: collection date

text: This sample is a virtual sample of raw reads from multiple physical samples of Ralstonia phage UAM5 genome assembly and is composed of two physical samples ERS8941345, ERS8941346.

characteristic: description

text: Colombia

characteristic: geographic location (country and/or sea)

text: Calcedonia, Valle

text: Plantain rhizosphere soil

characteristic: isolation source

text: Ralstonia solanacearum strain UA1591

characteristic: lab host

text: 4.303389, -75.885806

characteristic: lat lon

text: Ralstonia phage UAM5

text: ERS8941345, ERS8941346

characteristic: sample composed of

text: Ralstonia phage UAM5

text: UAM5 phage virtual sample

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Institut d'Investigacio Sanitaria de Palma

text: Derivative Pseudomonas aeruginosa mutant upon antibiotic presure

text: Spain

text: diseased

characteristic: host health state

text: human

characteristic: host scientific name

text: Pseudomonas aeruginosa

characteristic: isolate

text: Laboratory

text: 28.1248 15.43

text: Pseudomonas aeruginosa

characteristic: organism

text: Pseudomonas aeruginosa

text: ND

characteristic: serovar

text: ND

characteristic: strain


text: Institut d'Investigacio Sanitaria de Palma

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: Spain

text: diseased

text: human

text: Pseudomonas aeruginosa

text: Laboratory

text: 28.1248 15.43

text: Pseudomonas aeruginosa

text: Pseudomonas aeruginosa

text: ND

text: ND


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK)

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: primary cell line

characteristic: cell line

text: house mouse

characteristic: common name

text: Protocols: Tumors from Ptf1aCre/+;KrasG12D/+ or Pdx1-Cre;KrasG12D/+ mice were removed and digested in 10 mL Dulbecco's modified Eagle medium (DMEM) containing 150 U/mL collagenase Type 2 (Worthington, Lakewood, NJ) as previously described. Digested tumors were dispersed into single cell suspension, resuspended in DMEM medium containing 10% fetal calf serum (FCS), and cultivated as described previously. All human cell lines were obtained from ATCC except the primary dispersed human PDAC primary cell lines, Loh-C and SMJ31, which were obtained from pancreatic ductal adenocarcinoma resections. After 24 hours of drug treatment, when the primary cell cultures were 80% confluent, the cells were immediately transferred into RLT buffer (Qiagen) containing β-mercaptoethanol and the RNA was isolated according to the Qiagen, RNeasy kit, instructions. For drug treated cells only as indicated in the sample attributes. Cells were seeded in a 10 cm dish, the following day the cells were treated with Trametinib, 10 nanomolar, or vehicle (DMSO) for 24 hours. RNA was isolated with the RNeasy kit (Qiagen). Library preparation for bulk-sequencing of poly(A)-RNA by UMI-seq was done as described previously (Parekh et al., 2016). Briefly, barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adaptor. Ends of the cDNAs were extended by a template switch oligo (TSO) and full-length cDNA was amplified with primers binding to the TSO-site and the adaptor. NEB UltraII FS kit was used to fragment cDNA. After end repair and A-tailing a TruSeq adapter was ligated and 3’-end-fragments were finally amplified using primers with Illumina P5 and P7 overhangs. In comparison to Parekh et al. (2016), the P5 and P7 sites were exchanged to allow sequencing of the cDNA in read1 and barcodes and UMIs in read2 to achieve a better cluster recognition.

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

characteristic: disease

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus

text: C57BL/6 / 129S1/SvImJ

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: primary cell line

text: house mouse

text: adult

text: pancreatic ductal adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus

text: C57BL/6 / 129S1/SvImJ

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary cell line

text: house mouse

characteristic: description

text: adult

text: pancreatic ductal adenocarcinoma

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

text: female

characteristic: sex

text: C57BL/6 / 129S1/SvImJ


text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: primary cell line

characteristic: cell line

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

characteristic: disease

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

text: male

text: C57BL/6 / 129S1/SvImJ


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: primary cell line

text: house mouse

text: adult

text: pancreatic ductal adenocarcinoma

text: Mus musculus

text: Mus musculus

text: pancreas

characteristic: organism part

text: Mus musculus

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary cell line

text: house mouse

text: adult

text: pancreatic ductal adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus

text: male

characteristic: strain


text: public

text: ArrayExpress

characteristic: broker name

text: primary cell line

characteristic: cell line

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

unit: year

characteristic: age

text: ArrayExpress

text: human

text: adult

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: European

characteristic: ethnic group

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: cystic fibrosis patient

characteristic: clinical information

text: human

text: adult

text: pancreatic ductal adenocarcinoma

text: European

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

characteristic: organism part

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: Dan-G

characteristic: cell line

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

text: European

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: female

characteristic: sex

text: DanG


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: age

text: ArrayExpress

text: HPAC

text: human

text: adult

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: European

characteristic: ethnic group

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: female

text: HPAC


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: HPAFII

text: human

text: adult

text: pancreatic ductal adenocarcinoma

text: European

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

characteristic: organism part

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: Hs 766T

characteristic: cell line

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

text: European

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: age

text: ArrayExpress

text: human

text: adult

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: Japanese

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: pancreatic ductal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

characteristic: broker name

text: primary cell line

characteristic: cell line

text: human

characteristic: common name

text: adult

text: pancreatic ductal adenocarcinoma

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

characteristic: organism part

text: Homo sapiens

text: Loh-C

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: MIA Paca-2

text: hypotriploid human cell line

characteristic: clinical information

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

text: European

characteristic: ethnic group

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: human

text: adult

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

characteristic: common name

text: adult

text: pancreatic ductal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: primary cell line

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: pancreatic ductal adenocarcinoma

text: Mus musculus

characteristic: organism

text: Mus musculus

text: pancreas

text: Mus musculus

text: female

text: C57BL/6 / 129S1/SvImJ

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: primary cell line

text: house mouse

text: adult

text: pancreatic ductal adenocarcinoma

characteristic: disease

text: Mus musculus

text: Mus musculus

text: pancreas

text: Mus musculus

text: male

text: C57BL/6 / 129S1/SvImJ


characteristic: ENA-LAST-UPDATE

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell line

characteristic: cell line

text: human

characteristic: common name

text: adult

text: pancreatic ductal adenocarcinoma

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: Homo sapiens


text: Enterobacter cloacae

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

characteristic: genus

characteristic: isolate

text: Enterobacter cloacae

text: Enterobacter cloacae

characteristic: title

type: AMR curated data - CABBAGE

value: gentamicin

value: EUCAST

value: broth dilution

value: Vitek

value: susceptible

value: Enterobacter cloacae

value: piperacillin-tazobactam

value: resistant

value: tobramycin

value: ampicillin

value: cefpodoxime-clavulanic acid

value: meropenem

value: intermediate

value: amikacin

value: amoxicillin-clavulanic acid

value: tigecycline

value: cefoxitin

value: cefotaxime

value: ertapenem

value: cefpodoxime


text: Enterobacter cloacae

characteristic: ArrayExpress-SPECIES

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Enterobacter cloacae

text: Enterobacter cloacae

characteristic: organism

text: Enterobacter cloacae

characteristic: subject id


text: Enterobacter cloacae

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: IRL

characteristic: SRA accession

characteristic: Submitter Id

text: Enterobacter cloacae

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

value: EUCAST

value: broth dilution

value: Vitek

value: Enterobacter hormaechei


text: Enterobacter cloacae

characteristic: ArrayExpress-Species

text: SC

text: public

text: IRL

text: Enterobacter cloacae

characteristic: common name

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

value: susceptible

value: Enterobacter cloacae

value: gentamicin

value: resistant

value: piperacillin-tazobactam


text: Enterobacter cloacae

characteristic: ENA-CHECKLIST

characteristic: External Id

text: SC

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

value: cefpodoxime-clavulanic acid

value: tobramycin

value: ampicillin

value: meropenem


text: Enterobacter cloacae

text: SC

characteristic: INSDC center name

text: public

text: Enterobacter cloacae

text: Enterobacter cloacae

text: Enterobacter cloacae


text: Enterobacter cloacae

text: SC

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

characteristic: genus

characteristic: isolate

text: Enterobacter cloacae

text: Enterobacter cloacae

value: amikacin

value: cefoxitin

value: amoxicillin-clavulanic acid

value: tigecycline


text: Enterobacter cloacae

text: SC

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

characteristic: title

value: EUCAST

value: cefpodoxime

value: cefotaxime

value: ertapenem


text: Enterobacter cloacae

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

value: broth dilution

value: Vitek

value: susceptible

value: Enterobacter hormaechei


text: Enterobacter cloacae

text: SC

characteristic: INSDC first public

text: public

text: IRL

text: Enterobacter cloacae

text: Enterobacter

text: Enterobacter cloacae

text: Enterobacter cloacae

value: resistant

value: piperacillin-tazobactam

value: gentamicin


text: Fondation Merieux

characteristic: INSDC last update

text: public

tag: Namespace:Fondation Merieux

characteristic: collection date

text: ESBL-E. coli from human gut carriage

characteristic: description

text: Madagascar

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Yes

text: human

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY COLLEGE DUBLIN

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY COLLEGE DUBLIN

characteristic: Submitter Id

text: marine metagenome

text: eDNA

text: marine

characteristic: environment (biome)

text: coral reef

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Honduras

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

characteristic: organism

text: Probe capture of target DNA from eDNA samples

characteristic: project name

text: marine metagenome

text: Illumina NovaSeq (PE 2x250bp)

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:HELMHOLTZ CENTRE FOR INFECTION RESEARCH

characteristic: collection date

characteristic: description

text: human-gut

text: fecal

text: fecal

text: Germany

characteristic: geographic location (country and/or sea)

text: human-gut

characteristic: human gut environmental package

text: metatranscriptome

text: metagenome

text: Metatranscriptome analysis of fecal microbiota transplantation in chronic antibiotic resistant pouchitis

text: metagenome

text: Illumina HiSeq 2500

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-25-11-2021-23:24:52:109-12014

tag: Namespace:VISAVET Health Surveillance Centre (UCM)

characteristic: Submitter Id

text: CAMPY

text: Spain

text: healthy

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

characteristic: isolate

text: Wild boar

characteristic: isolation source

text: Campylobacter lanienae

text: Campylobacter lanienae


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: VISAVET Health Surveillance Centre (UCM)

text: public

text: ena-SAMPLE-TAB-25-11-2021-23:24:52:109-12015

text: CAMPY

text: Spain

text: healthy

text: Bos taurus

text: Cattle

text: Campylobacter fetus subsp. venerealis

characteristic: organism

text: Campylobacter fetus subsp. venerealis


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

characteristic: common name

text: Homo sapiens

text: HPAECs treated with miR-224-5p inhibitor to detect the differential expressed genes and the file alias was miR-A-1 ERC000011

characteristic: sample_description checklist_accession

text: Homo sapiens

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characteristic: ENA-LAST-UPDATE

text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR-224-5p inhibitor to detect the differential expressed genes and the file alias was miR-A-2 ERC000011

text: Homo sapiens

text: HPAECs treated with miR-224-5p inhibitor 2

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

text: human

text: Homo sapiens

text: HPAECs treated with miR-224-5p inhibitor to detect the differential expressed genes and the file alias was miR-A-3 ERC000011

text: Homo sapiens

text: HPAECs treated with miR-224-5p inhibitor 3


text: public

characteristic: Submitter Id

text: human

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor to detect the differential expressed genes and the file alias was miR-B-1 ERC000011

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor 1


text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor to detect the differential expressed genes and the file alias was miR-B-2 ERC000011

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor 2


text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor to detect the differential expressed genes and the file alias was miR-B-3 ERC000011

text: Homo sapiens

text: HPAECs treated with miR-361-3p inhibitor 3


text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR inhibitor negative control to detect the differential expressed genes and the file alias was Con-1 ERC000011

text: Homo sapiens

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text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR inhibitor negative control to detect the differential expressed genes and the file alias was Con-2 ERC000011

text: Homo sapiens

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text: public

text: human

text: Homo sapiens

text: HPAECs treated with miR inhibitor negative control to detect the differential expressed genes and the file alias was Con-3 ERC000011

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text: Ravi Kant

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tag: Namespace:Ravi Kant

text: HUSLAB

characteristic: collecting institution

characteristic: collection date

text: not provided

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text: Finland

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text: human

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text: Homo sapiens

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text: male

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text: not provided

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text: not provided

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

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text: not provided

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text: ETH Zurich & University of Geneva

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text: ArrayExpress

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text: plasma cell

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text: Protocols: For protein immunizations, a 6-week-old male C57BL/6 mouse was repeatedly immunized five times every other week s.c. into the flank with 10 μg of human TNFR2 protein (Peprotech, 310-12)/20 μg MPLA (Sigma, L6895) adjuvant and sacrificed 1 week afterwards. Likewise, a 6-week-old female BALB/c mouse was injected three times with 4 and 3 weeks intervals s.c. into the flank with 100 μg of OVA (Sigma, A5503)/20 μg MPLA (Sigma, L6895) adjuvant and sacrificed 2 weeks after the final boost. Bone marrow plasma cells were isolated either using CD138+ FACS or MACS following infections or immunizations. All experiments were performed according to 10x 5' immune profiling. Single-cell 10× VDJ libraries were constructed from the isolated BM plasma cells following the demonstrated protocol “Direct target enrichment -Chromium Single Cell V(D)J Reagent Kits” (CG000166 REV A). Briefly, single cells were co-encapsulated with gel beads (10× Genomics, 1000006) in droplets using three lanes of one Chromium Single-Cell A Chip (10× Genomics, 1000009) with a target loading of 13,000 cells per reaction. V(D)J library construction was carried out using the Chromium Single Cell 5’ Library Kit (10× Genomics, 1000006) and the Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell (10× Genomics, 1000072).

characteristic: description

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text: ETH Zurich & University of Geneva

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text: plasma cell

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text: Protocols: LCMV Infection was performed i.v. (tail vein) with 2x10e6 FFU of clone 13 LCMV. OVA immunizations were administered every two weeks with 100ug OVA with 20ug Monophosphoryl lipid A (MPLA) subcutaneous. hTNFR2 immunizations were performed with 5 administrations every two weeks with 10ug TNFR2 and 20ug MPLA. Bone marrow plasma cells were isolated either using CD138+ FACS or MACS following infections or immunizations. All experiments were performed according to 10x 5' immune profiling. Single-cell 10× VDJ libraries were constructed from the isolated BM plasma cells following the demonstrated protocol “Direct target enrichment -Chromium Single Cell V(D)J Reagent Kits” (CG000166 REV A). Briefly, single cells were co-encapsulated with gel beads (10× Genomics, 1000006) in droplets using three lanes of one Chromium Single-Cell A Chip (10× Genomics, 1000009) with a target loading of 13,000 cells per reaction. V(D)J library construction was carried out using the Chromium Single Cell 5’ Library Kit (10× Genomics, 1000006) and the Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell (10× Genomics, 1000072).

text: adult

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text: Mus musculus

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text: Yannick Gerth

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text: Yannick Gerth

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characteristic: organism


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characteristic: GISAID Accession ID

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text: public

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text: Yannick Gerth

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characteristic: organism


characteristic: GISAID Accession ID

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text: public

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characteristic: GISAID Accession ID

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text: public

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text: Yannick Gerth

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characteristic: organism

text: active surveillance in response to outbreak


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text: Center for Laboratory Medicine, St. Gallen, Switzerland

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characteristic: GISAID Accession ID

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text: restricted access

text: restricted access

text: hCoV-19/Switzerland/SG-CLM-11159045/2021

characteristic: organism


characteristic: GISAID Accession ID

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text: public

text: SIB

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text: Homo sapiens

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characteristic: organism


E-MTAB-111261637921679:5percentO2 1 608

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:5percentO2 1 608

tag: Namespace:DANA-FARBER CANCER INSTITUTE

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cells are scraped from 6 well plates. All source cell culture was maintained in media containing plasmocin (1:5000 dilution, Invivogen) to prevent mycoplasma contamination. Culture plates were briefly coated with 40μg/ml of fibronectin (Sigma) and glass coverslips were incubated in 40 μg/ml fibronectin for at least 2 hours. Human mammary epithelial MCF10A cells were maintained at 370C with 5% CO2 atmosphere and cultured as previously described18. In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Telomerase-immortalized RPE-1 cells (ATCC), and all derivative cell lines generated in this study were cultured in phenol red-free DMEM/F12 media containing 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin. At day 0, control 608 and PLK4 cells were gently dissociated into single cells, then seeded at low density into media with 2μg/ml of doxycycline such that they were ~80% confluent on day 5. After ~48 hours of doxycycline incubation, the culture media with doxycycline was replaced. On day 5, cells are gently dissociated into single cells before reseeding into 6 well plates to ~80% confluent on day 7. Because control 608 cells grow faster than PLK4 induced cells, control 608 cells are seeded at ~2.5 times lower density than PLK4 induced cells. Cells at ~80% confluency are analysed either at day 7 or day 8. Media is left unchanged for ~3 days before analysis. To conditionally overexpress PLK4, lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and pLenti-CMV/TO-Neo-Dest (17292, Addgene) were used. Wild-type PLK4 and PLK4 1-608 cDNAs (control 608) were cloned using the Gateway system into the pLenti-CMV/TO-Neo-Dest vector Cells were coinfected with a lentivirus containing the TetR and with the lentivirus containing the wild-type PLK4 or control 608 open reading frames. Transduced cells were selected with Blasticidin (10 μg/ml) and Geneticin (200μg/ml). control 608 and PLK4 overexpression was induced transiently. Transduced cells that survive the selection are used for subsequent experiments and a maintenance dose of 5 μg/ml of Blasticidin and 80μg/ml of Geneticin was used in stock cultures. MCF10A cells were exposed to 12Gy gamma irradiation RNeasy kit was used to extract the RNA from the cell lysates NextSeq500 RNA Library Preparation and Sequencing RNA quantity was determined on the Qubit using the Qubit RNA Assay Kit (Life Tech) and RNA quality was determined on the Bioanalyzer using the RNA Pico Kit (Agilent). Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A) mRNA, 100ng of total RNA was used for cDNA library construction, according to the 'manufacturer's protocol.

characteristic: description

text: normal

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text: PLK4-608 mutant transient overexpression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: retina

characteristic: organism part

text: Homo sapiens

text: 5% O2

characteristic: stimulus

text: 5percentO2 1 608


E-MTAB-111261637921679:5percentO2 2 608

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

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text: public

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text: E-MTAB-111261637921679:5percentO2 2 608

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: 5% O2

text: 5percentO2 2 608

characteristic: title


E-MTAB-111261637921679:5percentO2 3 608

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text: ArrayExpress

text: epithelial cell

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: retina

text: Homo sapiens

text: 5% O2

text: 5percentO2 3 608


text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:5percentO2 4 PLK4

text: ArrayExpress

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: PLK4 mutant transient overexpression

text: Homo sapiens

text: Homo sapiens

text: retina

text: Homo sapiens

text: 5% O2

text: 5percentO2 4 PLK4


text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:5percentO2 5 PLK4

text: ArrayExpress

text: epithelial cell

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text: PLK4 mutant transient overexpression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: retina

characteristic: organism part

text: Homo sapiens

text: 5% O2

characteristic: stimulus

text: 5percentO2 5 PLK4


text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-111261637921679:5percentO2 6 PLK4

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

text: normal

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text: retina

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text: 5% O2

text: 5percentO2 6 PLK4


E-MTAB-111261637921679:Catalase 608_1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:Catalase 608_1

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: Protocols: Cells are scraped from 6 well plates. All source cell culture was maintained in media containing plasmocin (1:5000 dilution, Invivogen) to prevent mycoplasma contamination. Culture plates were briefly coated with 40μg/ml of fibronectin (Sigma) and glass coverslips were incubated in 40 μg/ml fibronectin for at least 2 hours. Human mammary epithelial MCF10A cells were maintained at 370C with 5% CO2 atmosphere and cultured as previously described18. In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Telomerase-immortalized RPE-1 cells (ATCC), and all derivative cell lines generated in this study were cultured in phenol red-free DMEM/F12 media containing 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin. At day 0, control 608 and PLK4 cells were gently dissociated into single cells, then seeded at low density into media with 2μg/ml of doxycycline such that they were ~80% confluent on day 5. After ~48 hours of doxycycline incubation, the culture media with doxycycline was replaced. On day 5, cells are gently dissociated into single cells before reseeding into 6 well plates to ~80% confluent on day 7. Because control 608 cells grow faster than PLK4 induced cells, control 608 cells are seeded at ~2.5 times lower density than PLK4 induced cells. Cells at ~80% confluency are analysed either at day 7 or day 8. Media is left unchanged for ~3 days before analysis. RPE-1 cells grown in 5% oxygen chamber To conditionally overexpress PLK4, lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and pLenti-CMV/TO-Neo-Dest (17292, Addgene) were used. Wild-type PLK4 and PLK4 1-608 cDNAs (control 608) were cloned using the Gateway system into the pLenti-CMV/TO-Neo-Dest vector Cells were coinfected with a lentivirus containing the TetR and with the lentivirus containing the wild-type PLK4 or control 608 open reading frames. Transduced cells were selected with Blasticidin (10 μg/ml) and Geneticin (200μg/ml). control 608 and PLK4 overexpression was induced transiently. Transduced cells that survive the selection are used for subsequent experiments and a maintenance dose of 5 μg/ml of Blasticidin and 80μg/ml of Geneticin was used in stock cultures. MCF10A cells were exposed to 12Gy gamma irradiation RNeasy kit was used to extract the RNA from the cell lysates NextSeq500 RNA Library Preparation and Sequencing RNA quantity was determined on the Qubit using the Qubit RNA Assay Kit (Life Tech) and RNA quality was determined on the Bioanalyzer using the RNA Pico Kit (Agilent). Using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB), which selects for poly(A) mRNA, 100ng of total RNA was used for cDNA library construction, according to the 'manufacturer's protocol.

text: normal

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Catalase 608_1

characteristic: title


E-MTAB-111261637921679:Catalase 608_2

text: DANA-FARBER CANCER INSTITUTE

text: public

text: E-MTAB-111261637921679:Catalase 608_2

text: ArrayExpress

text: MCF 10A

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Catalase 608_2


E-MTAB-111261637921679:Catalase 608_3

text: DANA-FARBER CANCER INSTITUTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-111261637921679:Catalase 608_3

text: ArrayExpress

text: MCF 10A

text: epithelial cell

text: human

text: normal

characteristic: disease

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Catalase 608_3