characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSD Health

text: public

text: terminal ileum

text: western European house mouse

text: normal chow, ad-libitum, timepoint 5

characteristic: description

text: normal chow

text: WT

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mus musculus domesticus

characteristic: organism

text: ad libitum feeding

characteristic: project name

text: Mus musculus domesticus

text: male


status: PUBLIC

tag: attribute

text: UCSD Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UCSD Health

characteristic: Submitter Id

text: terminal ileum

characteristic: body site

characteristic: collection date

text: western European house mouse

characteristic: common name

text: normal chow, ad-libitum, timepoint 6

text: normal chow

characteristic: diet

text: WT

characteristic: genotype

text: USA

text: Mus musculus domesticus

text: ad libitum feeding

characteristic: perturbation

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: strain

characteristic: title

characteristic: zt time


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSD Health

text: public

text: terminal ileum

text: western European house mouse

text: normal chow, time-restricted, timepoint 1

characteristic: description

text: normal chow

text: WT

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mus musculus domesticus

characteristic: organism

text: time-restricted feeding

characteristic: project name

text: Mus musculus domesticus

text: male


text: UCSD Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: terminal ileum

characteristic: body site

characteristic: collection date

text: western European house mouse

characteristic: common name

text: normal chow, time-restricted, timepoint 2

text: normal chow

characteristic: diet

text: WT

text: USA

text: Mus musculus domesticus

text: time-restricted feeding

text: Mus musculus domesticus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSD Health

text: public

text: terminal ileum

text: western European house mouse

text: normal chow, time-restricted, timepoint 3

text: normal chow

text: WT

characteristic: genotype

text: USA

text: Mus musculus domesticus

text: time-restricted feeding

characteristic: perturbation

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: strain

characteristic: title

characteristic: zt time


text: UCSD Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: terminal ileum

text: western European house mouse

text: normal chow, time-restricted, timepoint 4

characteristic: description

text: normal chow

text: WT

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Mus musculus domesticus

characteristic: organism

text: time-restricted feeding

characteristic: project name

text: Mus musculus domesticus

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSD Health

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: terminal ileum

characteristic: body site

characteristic: collection date

text: western European house mouse

characteristic: common name

text: normal chow, time-restricted, timepoint 5

text: normal chow

characteristic: diet

text: WT

text: USA

text: Mus musculus domesticus

text: time-restricted feeding

text: Mus musculus domesticus

text: male


text: UCSD Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: terminal ileum

text: western European house mouse

text: normal chow, time-restricted, timepoint 6

text: normal chow

text: WT

characteristic: genotype

text: USA

text: Mus musculus domesticus

text: time-restricted feeding

characteristic: perturbation

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: strain

characteristic: title

characteristic: zt time


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: COVID-HUB-PL

text: public

text: GENESIS

characteristic: collecting institution

text: prof. UM dr hab. med. Aleksander Jamsheer

characteristic: collector name

text: Poland

characteristic: geographic location (country and/or sea)

text: not provided

unit: DD

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: not provided

characteristic: geographic location (region and locality)

unit: year

characteristic: host age

text: human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: receipt date

text: active surveillance in response to outbreak

characteristic: sample capture status

text: COVID-HUB-PL deep NGS sequencing of SARS-CoV-2 genomes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:EUROPEAN RESEARCH INSTITUTE FOR THE BIOLOGY OF AGEING, UNIVERSITY OF GRONINGEN, UNIVERSITY MEDICAL CENTER GRONINGEN

characteristic: Submitter Id

text: human

characteristic: common name

text: Wildtype fallopian tube epithelial cell line; replicate 1

characteristic: description

text: Homo sapiens

text: Homo sapiens

characteristic: title


text: EUROPEAN RESEARCH INSTITUTE FOR THE BIOLOGY OF AGEING, UNIVERSITY OF GRONINGEN, UNIVERSITY MEDICAL CENTER GRONINGEN

text: public

text: human

text: Wildtype fallopian tube epithelial cell line; replicate 2

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53 single-mutant fallopian tube epithelial cell line

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; empty vector fallopian tube epithelial cell line

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; MYC overexpressing fallopian tube epithelial cell line

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; fallopian tube epithelial cell line

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; fallopian tube epithelial cell line; diploid cells

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; fallopian tube epithelial cell line; tetraploid cells

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; empty vector fallopian tube epithelial cell line; diploid cells

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; empty vector fallopian tube epithelial cell line; tetraploid cells

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; MYC overexpressing fallopian tube epithelial cell line; diploid cells

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: TP53/BRCA1 double-mutant; MYC overexpressing fallopian tube epithelial cell line; tetraploid cells

text: Homo sapiens

text: Homo sapiens


text: INSTITUTE OF EVOLUTIONARY BIOLOGY

text: public

tag: Namespace:INSTITUTE OF EVOLUTIONARY BIOLOGY

text: African malaria mosquito

text: Anopheles funestus

text: Anopheles funestus


text: INIAV

text: public

text: INIAV

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

text: Portugal

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: healthy

characteristic: host health state

text: sus

characteristic: host scientific name

text: No

characteristic: is the sequenced pathogen host associated?

text: Escherichia coli

characteristic: isolate

text: Escherichia coli

text: Escherichia coli

text: E. coli


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:ETH Zurich

text: Bacterial communities of soil, and of the phyllosphere of Arabidopsis thaliana. Plants were cultivated in a common garden experiment outside, and in gnotobiotic conditions.

text: Arabidopsis thaliana Col0

characteristic: ecotype

text: Arabidopsis thaliana

text: Soil, leaves, and flowers

text: bacterium

characteristic: organism

text: bacterium

text: Soil and phyllosphere microbiota


text: Sciensano

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Jessy Praet

characteristic: collected by

characteristic: collection date

text: Bombus hypnorum

characteristic: isolation source

text: Snodgrassella alvi

text: Snodgrassella alvi

characteristic: strain

text: Bumblebee gut

characteristic: tissue type

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Sciensano

text: public

text: Jessy Praet

text: Bombus lapidarius

text: Snodgrassella alvi

text: Snodgrassella alvi

text: Bumblebee gut


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sciensano

text: public

text: Jessy Praet

text: Bombus lucorum

text: Snodgrassella alvi

text: Snodgrassella alvi

text: Bumblebee gut


text: Sciensano

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Jessy Praet

text: Bombus pascuorum

text: Snodgrassella alvi

text: Snodgrassella alvi

text: Bumblebee gut


text: Sciensano

characteristic: INSDC last update

text: public

text: Jessy Praet

text: Bombus terrestris

text: Snodgrassella alvi

text: Snodgrassella alvi

text: Bumblebee gut


LMG 28360

text: Sciensano

text: public

text: LMG 28360

text: Jessy Praet

text: Bombus terrestris

text: Snodgrassella alvi

characteristic: organism

text: Snodgrassella alvi

text: LMG 28360

text: Bumblebee gut

text: LMG 28360


LMG 30236

text: Sciensano

text: public

characteristic: INSDC status

text: LMG 30236

text: Jessy Praet

text: Bombus pascuorum

text: Snodgrassella alvi

text: Snodgrassella alvi

text: LMG 30236

text: Bumblebee gut

text: LMG 30236


text: public

characteristic: SRA accession

tag: Namespace:SCIENCE FOR LIFE LABORATORY, KTH ROYAL INSTITUTE OF TECHNOLOGY

text: 9 marine samples sequenced with 10 short-read primer pairs on Illumina Miseq, 3 long-read pairs on PacBio Sequel and with shotgun MG

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: marine water body (ENVO:00001999)

characteristic: environment (feature)

text: saline water (ENVO:00002010)

characteristic: environment (material)

text: North Sea

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

characteristic: pcr primers

text: Evaluation of primers targeting rRNA genes for metabarcoding of eukaryotes

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 18S rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SCIENCE FOR LIFE LABORATORY, KTH ROYAL INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: North Sea

text: eukaryote

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Sequencing by synthesis (Illumina)

text: 18S rRNA

characteristic: title

text: water


text: public

characteristic: SRA accession

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

characteristic: pcr primers

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (Illumina)

text: 18S rRNA

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: Baltic Sea

text: eukaryote

text: marine metagenome

text: marine metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 18S rRNA

characteristic: target gene

characteristic: target subfragment

text: water

characteristic: water environmental package


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: marine water body (ENVO:00001999)

characteristic: environment (feature)

text: saline water (ENVO:00002010)

characteristic: environment (material)

text: North Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: eukaryote

text: marine metagenome

characteristic: organism

text: V4F_Balzano- 28S_21R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D8)

text: V4F_Balzano- 28S_21R

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: North Sea

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

text: V4F_Balzano- 28S_3143R

characteristic: pcr primers

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D10)

text: V4F_Balzano- 28S_3143R

text: water


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: marine water body (ENVO:00001999)

characteristic: environment (feature)

text: saline water (ENVO:00002010)

characteristic: environment (material)

text: North Sea

characteristic: geographic location (country and/or sea)

text: eukaryote

text: marine metagenome

text: V4F_Balzano- 28S_2742R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

characteristic: sequencing method

text: rRNA operon (18S-28S)

characteristic: target gene

text: 18S (V4)- 28S (D8)

characteristic: target subfragment

text: V4F_Balzano- 28S_2742R

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: eukaryote

text: marine metagenome

characteristic: organism

text: V4F_Balzano- 28S_21R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D8)

text: V4F_Balzano- 28S_21R

characteristic: title

text: water


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

text: V4F_Balzano- 28S_3143R

characteristic: pcr primers

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D10)

text: V4F_Balzano- 28S_3143R

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: eukaryote

text: marine metagenome

text: V4F_Balzano- 28S_2742R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

characteristic: sequencing method

text: rRNA operon (18S-28S)

characteristic: target gene

text: 18S (V4)- 28S (D8)

characteristic: target subfragment

text: V4F_Balzano- 28S_2742R

text: water

characteristic: water environmental package


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: marine water body (ENVO:00001999)

characteristic: environment (feature)

text: saline water (ENVO:00002010)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: eukaryote

text: marine metagenome

characteristic: organism

text: V4F_Balzano- 28S_21R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D8)

text: V4F_Balzano- 28S_21R

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: Baltic Sea

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

text: V4F_Balzano- 28S_3143R

characteristic: pcr primers

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (PacBio)

text: rRNA operon (18S-28S)

text: 18S (V4)- 28S (D10)

text: V4F_Balzano- 28S_3143R

text: water


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: marine water body (ENVO:00001999)

characteristic: environment (feature)

text: saline water (ENVO:00002010)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

text: eukaryote

text: marine metagenome

text: V4F_Balzano- 28S_2742R

text: marine metagenome

text: Sequencing by synthesis (PacBio)

characteristic: sequencing method

text: rRNA operon (18S-28S)

characteristic: target gene

text: 18S (V4)- 28S (D8)

characteristic: target subfragment

text: V4F_Balzano- 28S_2742R

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: North Sea

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: eukaryote

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Sequencing by synthesis (Illumina)

characteristic: title

text: water


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: eukaryote

characteristic: investigation type

text: marine metagenome

characteristic: project name

text: marine metagenome

text: Sequencing by synthesis (Illumina)

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: marine pelagic biome (ENVO:01000023)

text: marine water body (ENVO:00001999)

text: saline water (ENVO:00002010)

text: Baltic Sea

text: eukaryote

text: marine metagenome

text: marine metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: water

characteristic: water environmental package


text: UPPSALA UNIVERISTY

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UPPSALA UNIVERISTY

characteristic: Submitter Id

unit: L

characteristic: amount or size of sample collected

text: Shadi Eshghi Sahraei, Mustafa Zakieh

characteristic: collected by

characteristic: collection date

text: Amplicon library from soil close to a Fritillaria meleagris plant in mesic-dry area. Approximately 1500bp of the rRNA was amplified using primers ITS1 (slightly modified) and LR5, and sequenced on a PacBio RSII system.

characteristic: description

text: soil

text: Sweden

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

text: Uppsala, Kungsängen

characteristic: geographic location (region and locality)

text: mimarks-survey

text: soil metagenome

characteristic: organism

text: initial denaturation:95degC_10min; denaturation:95degC_0.75min; annealing:59degC_0.75min; elongation:72degC_1.5min; total cycles:25; final elongation:72degC_10min

characteristic: pcr conditions

text: FWD: TCCGTAGGTGAACCTGC REV: TCCTGAGGGAAACTTCG

characteristic: pcr primers

text: Grassland above and below ground community shift across soil moisture transition

text: Soil samples were individually placed in plastic bags and kept on ice during sampling before storage at 4degC over night. The day after, soils were homogenized in the plastic bags and subsamples were transferred to 15ml conical centrifuge tubes and frozen at -20degC prior to freeze drying

characteristic: sample material processing

unit: g

characteristic: sample weight for DNA extraction

text: soil metagenome

text: PacBio

text: soil

characteristic: soil environmental package

text: rRNA

characteristic: target gene

text: ITS1;5.8S;ITS2;LSU rDNA

characteristic: target subfragment

text: Amplicon library from soil in mesic-dry area

characteristic: title

unit: g/g

characteristic: water content

text: Subsample was weighed (wet weight) before drying at +80 deg C for 48 h (dry weight). The gravimetric soil moisture was estimated using the equation: (gravimetric soil moisture) = (wet weight - dry weight)/(dry weight). Ref: Holliday, V.T. (1990) Methods of soil analysis, part 1, physical and mineralogical methods , A. Klute, Ed., 1986, American Society of Agronomy, Agronomy Monographs 9 (1), Madison, Wisconsin, 1188 pp., $60.00. In: Wiley Online Library.

characteristic: water content method


characteristic: ENA-CHECKLIST

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text: UPPSALA UNIVERISTY

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text: Shadi Eshghi Sahraei, Mustafa Zakieh

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text: Amplicon library from soil not close to a Fritillaria meleagris plant in mesic-dry area. Approximately 1500bp of the rRNA was amplified using primers ITS1 (slightly modified) and LR5, and sequenced on a PacBio RSII system.

characteristic: environment (biome)

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text: soil

characteristic: environment (material)

text: Sweden

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

text: Uppsala, Kungsängen

text: mimarks-survey

characteristic: investigation type

text: soil metagenome

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text: soil metagenome

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characteristic: sequencing method

text: soil

text: rRNA

text: ITS1;5.8S;ITS2;LSU rDNA


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text: Amplicon library from soil close to a Fritillaria meleagris plant in wet area. Approximately 1500bp of the rRNA was amplified using primers ITS1 (slightly modified) and LR5, and sequenced on a PacBio RSII system.

characteristic: description

text: soil

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characteristic: geographic location (depth)

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