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text: public

text: Random sampling

text: IMS-10186-CVDP-100FD3FB-A445-44F8-A040-A4C4D915C491

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-C9F5DFD3-81D3-41EC-A324-B9071BB1602E

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-6E68875F-DB0A-4C22-ADA4-9083EC00CFD8

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-AB9DDCD3-8F77-48CB-9815-83D18D6F44A4

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-479EB9B7-FCC9-46F6-B119-6C314BA8099C

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-EFD77453-82EF-4405-98F3-4B692D930B72

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-24607266-128F-4A51-9517-BB929C382AC5

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Molecular surveillance of SARS-CoV-2 in Germany


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-E93EF818-186C-40CD-BC10-235C860516C4

text: RKI-OSEDA

text: Labor ZOTZ|KLIMAS; MVZ Düsseldorf-Centrum, Immermannstr. 65a; 40210 Düsseldorf

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-02D0D2B3-FBD5-429D-B6CD-BAD51BE795DC

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

text: Severe acute respiratory syndrome coronavirus 2


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-914C1269-9720-4BB7-B8A2-7E1877B06652

text: RKI-OSEDA

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-0620B6E1-D17C-4271-BDD2-FF3C1AD42F44

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-8C39073A-64CA-46BE-A504-DD0F74C195CC

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Latvian Biomedical Research and Study Centre

text: Strain A, start phase, 0mM

characteristic: description

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: Latvian Biomedical Research and Study Centre

text: public

text: Strain B, start phase, 0mM

text: Kluyveromyces marxianus

characteristic: organism

text: Kluyveromyces marxianus


text: public

text: Strain C, start phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus

characteristic: title


characteristic: INSDC center name

text: public

text: Strain A, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


characteristic: INSDC first public

text: public

text: Strain A, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


characteristic: INSDC last update

text: public

text: Strain A, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

characteristic: INSDC status

text: Strain B, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

characteristic: SRA accession

text: Strain B, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, end phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

tag: Namespace:Department of Biology, University of Fribourg.

text: 50hr post-L1

unit: hour

characteristic: age

text: ArrayExpress

text: Protocols: At least 1000 animals from wild-type and both lsl-1(tm4769) and lsl-1(ljm-1) mutant strains were synchronized and collected as young adult hermaphrodites 50 hours post-larval hatching after hypochlorite treatment, at 20°C (Stiernagle, T. 2006)) Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA) and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad IA, USA) according to the manufacturer's instructions cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) by the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/)

text: adult

characteristic: developmental stage

characteristic: genotype

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Biology, University of Fribourg.

text: public

characteristic: Submitter Id

text: 50hr post-L1

text: ArrayExpress

characteristic: broker name

text: adult

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite


characteristic: External Id

characteristic: INSDC center name

text: public

text: 50hr post-L1

text: ArrayExpress

characteristic: description

text: adult

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: title


text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-A47ACC1A-6836-4076-BD93-ED7B15AC361E

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC center name

text: public

text: Random sampling

text: IMS-10186-CVDP-12D92D7F-60E8-45D2-8084-22BC1FD5F468

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


text: Robert Koch Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-2C20752A-05BA-49D4-92F9-3D77A0C8B5A8

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-08148594-7316-4B6C-8869-15C444D54D1E

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-F9F8798E-4A60-4A43-A6F3-2BF73AADC297

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-1F9257DF-4489-4393-A6D1-71C742057DDD

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

text: Molecular surveillance of SARS-CoV-2 in Germany


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Suspect sampling

text: IMS-10186-CVDP-81131A5E-074C-4F6D-A87D-4E546F6C360F

text: RKI-OSEDA

text: Labor ZOTZ|KLIMAS; MVZ Düsseldorf-Centrum, Immermannstr. 65a; 40210 Düsseldorf

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-148FBAE1-4864-403C-ABD8-DA0CB9BD5702

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: title


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-58598AED-8408-4F58-9EA8-5F5F51E7DA16

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-D5BB53B4-D92C-488D-8E43-DD895F26FA1D

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-DBCE1FDB-0FC0-403A-BBFB-FB81F62F8CDD

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Suspect sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-300DDE6B-9FA2-4464-B940-2D49A49EDA94

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-40CA7946-AE47-4CA4-8EDD-56A4149839CC

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-A2E44AAC-DE2F-4AA5-B48B-90CD19722753

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-C7B8485C-B5D0-41A5-ACC2-F0E607A8037F

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

text: IMS-10186-CVDP-1CF6839A-6A13-43E9-8CB7-C7B56BE4851C

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-AE09F141-22C6-4F22-8437-27C1885E6679

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-1EFF04A8-3749-4D79-B3EE-88C2E10C4010

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-E673AC74-6C12-4052-A32B-8BF49096548A

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-CE859FBB-E9CA-4287-8468-01A662C78728

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-312700C8-CCB6-44B3-AD35-2DEE0A6F5895

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Suspect sampling

text: IMS-10186-CVDP-E98B18B5-D239-4BD4-B24D-FD831796F4B3

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

text: IMS-10186-CVDP-0684727D-0093-4330-9D11-0C5812DD7D0D

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

text: public

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10186-CVDP-075BDBEE-0379-48F0-8D06-D1EFB1D8F11F

text: RKI-OSEDA

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Random sampling

text: IMS-10186-CVDP-3F05A7B5-55A4-401A-9898-B82AC8418B89

text: RKI-OSEDA

text: not provided

text: Germany

text: Europe/Germany/North Rhine-Westphalia

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status


E-MTAB-11206:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-11206:Sample 1

tag: Namespace:Molecular Genetics and Physiology of Plants, Ruhr University Bochum

characteristic: Submitter Id

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

characteristic: cultivar

text: Protocols: Shoot tissues of plants were cut and flash frozen in liquid nitrogen. Seeds were first germinated in agar-solidified 0.5x MS medium for 2 weeks before transfer into soil (Minitray, Balster Einheitserdewerk, Fröndenberg, DE) and a growth chamber at 12-h light(120 µmol photons m-2 s-1, 22°C) and 12-h dark (18°C; CLF Plant Climatics, Emersacker, Germany). Total RNA was isolated by RNeasy Plant Mini Kit (Qiagen). mRNA was enriched using oligo(dT) bead. The mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.

characteristic: description

text: LP.16 sixteen leaves visible stage

characteristic: developmental stage

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: shoot

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: Sample 1

characteristic: title


E-MTAB-11206:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Molecular Genetics and Physiology of Plants, Ruhr University Bochum

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11206:Sample 2

text: ArrayExpress

text: thale cress

text: LP.16 sixteen leaves visible stage

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 2


E-MTAB-11206:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

text: E-MTAB-11206:Sample 3

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: LP.16 sixteen leaves visible stage

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 3


E-MTAB-11206:Sample 4

text: public

text: E-MTAB-11206:Sample 4

text: ArrayExpress

text: thale cress

characteristic: common name

characteristic: cultivar

characteristic: description

text: LP.16 sixteen leaves visible stage

characteristic: developmental stage

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 4


E-MTAB-11206:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11206:Sample 5

text: ArrayExpress

text: thale cress

text: LP.16 sixteen leaves visible stage

text: not applicable

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: shoot

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: Sample 5

characteristic: title


E-MTAB-11206:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11206:Sample 6

text: ArrayExpress

text: thale cress

text: LP.16 sixteen leaves visible stage

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 6


E-MTAB-11206:Sample 7

text: public

characteristic: SRA accession

text: E-MTAB-11206:Sample 7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

characteristic: cultivar

text: LP.16 sixteen leaves visible stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 7


E-MTAB-11206:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11206:Sample 8

text: ArrayExpress

text: thale cress

characteristic: description

text: LP.16 sixteen leaves visible stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: Arabidopsis thaliana

text: Sample 8


E-MTAB-11206:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11206:Sample 9

text: ArrayExpress

text: thale cress

text: LP.16 sixteen leaves visible stage

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: shoot

characteristic: organism part

characteristic: replicate

text: Arabidopsis thaliana

text: Sample 9

characteristic: title


name: E-MTAB-11189:daf-16;daf-2 cdk-12 RNAi Rep-1

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Molecular Aging Laboratory, National Institute of Immunology

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Worms grown on control or cdk-12 RNAi were collected using 1XM9 buffer and washed thrice to remove bacterial contamination. 200 μl of Trizol reagent (Takara, Japan) was added to the 50μl of the worm pellet and subjected to three freeze-thaw cycles in liquid nitrogen with intermittent vertexing to break open worm bodies. The samples were then frozen in liquid nitrogen and stored in -80 °C till further use. 200 μl of Trizol was again added to the worm pellet and the sample was vortexed vigorously. To this, 200 μl of chloroform was added and tube was gently inverted several times followed by 3 minutes incubation at room temperature. Sample was then centrifuged at 12000g for 15 minutes at 4°C. The RNA containing upper aqueous phase was gently removed into a fresh tube without disturbing the bottom layer and interphase. To this aqueous solution, an equal volume of isopropanol was added and the reaction was allowed to sit for 10 minutes at room temperature followed by centrifugation at 12000g for 10 minutes at 4°C. The supernatant was carefully discarded without disturbing the RNA containing pellet. The pellet was washed using 1 ml 70% ethanol solution followed by centrifugation at 7500g for 15 minutes at 4°C. RNA pellet was further dried at room temperature and later dissolved in DEPC (diethyl pyrocarbonate) treated water followed by incubation at 65°C for 10 minutes with intermittent tapping. Concentration of RNA was determined by measuring absorbance at 260 nm using Nano Drop UV spectrophotometer (Thermo Scientific, USA) and quality checked using denaturing agarose formaldehyde gel. Libraries were constructed using NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog no-E7490L) and NEBNext® Ultra™ II Directional RNA Library Prep kit (Catalog no-E7765L) according to the manufacturer’s instructions.

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

text: daf-16;daf-2 cdk-12 RNAi Rep-1


name: E-MTAB-11189:daf-16;daf-2 cdk-12 RNAi Rep-2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Molecular Aging Laboratory, National Institute of Immunology

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-16;daf-2 cdk-12 RNAi Rep-2


name: E-MTAB-11189:daf-16;daf-2 control RNAi Rep-1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: L4 larva

characteristic: developmental stage

characteristic: genotype

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-16;daf-2 control RNAi Rep-1


name: E-MTAB-11189:daf-16;daf-2 control RNAi Rep-2

characteristic: INSDC center name

text: public

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-16;daf-2 control RNAi Rep-2

characteristic: title


E-MTAB-11189:daf-2 cdk-12 RNAi Rep-1

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11189:daf-2 cdk-12 RNAi Rep-1

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 cdk-12 RNAi Rep-1


E-MTAB-11189:daf-2 cdk-12 RNAi Rep-2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11189:daf-2 cdk-12 RNAi Rep-2

characteristic: Submitter Id

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

text: daf-2 cdk-12 RNAi Rep-2


E-MTAB-11189:daf-2 cdk-12 RNAi Rep-3

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11189:daf-2 cdk-12 RNAi Rep-3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Protocols: Worms grown on control or cdk-12 RNAi were collected using 1XM9 buffer and washed thrice to remove bacterial contamination. 200 μl of Trizol reagent (Takara, Japan) was added to the 50μl of the worm pellet and subjected to three freeze-thaw cycles in liquid nitrogen with intermittent vertexing to break open worm bodies. The samples were then frozen in liquid nitrogen and stored in -80 °C till further use. 200 μl of Trizol was again added to the worm pellet and the sample was vortexed vigorously. To this, 200 μl of chloroform was added and tube was gently inverted several times followed by 3 minutes incubation at room temperature. Sample was then centrifuged at 12000g for 15 minutes at 4°C. The RNA containing upper aqueous phase was gently removed into a fresh tube without disturbing the bottom layer and interphase. To this aqueous solution, an equal volume of isopropanol was added and the reaction was allowed to sit for 10 minutes at room temperature followed by centrifugation at 12000g for 10 minutes at 4°C. The supernatant was carefully discarded without disturbing the RNA containing pellet. The pellet was washed using 1 ml 70% ethanol solution followed by centrifugation at 7500g for 15 minutes at 4°C. RNA pellet was further dried at room temperature and later dissolved in DEPC (diethyl pyrocarbonate) treated water followed by incubation at 65°C for 10 minutes with intermittent tapping. Concentration of RNA was determined by measuring absorbance at 260 nm using Nano Drop UV spectrophotometer (Thermo Scientific, USA) and quality checked using denaturing agarose formaldehyde gel. EtOH precipitated total RNA samples - Library: Truseq stranded mRNA library (for human/animal/plant) - Platform: NovaSeq6000

characteristic: description

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 cdk-12 RNAi Rep-3


E-MTAB-11189:daf-2 cdk-12 RNAi Rep-4

characteristic: External Id

text: public

text: E-MTAB-11189:daf-2 cdk-12 RNAi Rep-4

text: ArrayExpress

text: L4 larva

characteristic: developmental stage

characteristic: genotype

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 cdk-12 RNAi Rep-4


E-MTAB-11189:daf-2 control RNAi Rep-1

characteristic: INSDC center name

text: public

text: E-MTAB-11189:daf-2 control RNAi Rep-1

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 control RNAi Rep-1

characteristic: title


E-MTAB-11189:daf-2 control RNAi Rep-2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11189:daf-2 control RNAi Rep-2

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 control RNAi Rep-2


E-MTAB-11189:daf-2 control RNAi Rep-3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11189:daf-2 control RNAi Rep-3

characteristic: Submitter Id

text: ArrayExpress

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

text: daf-2 control RNAi Rep-3


E-MTAB-11189:daf-2 control RNAi Rep-4

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11189:daf-2 control RNAi Rep-4

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: L4 larva

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: daf-2 control RNAi Rep-4


E-MTAB-11189:WT cdk-12 RNAi Rep-3

characteristic: External Id

text: public

text: E-MTAB-11189:WT cdk-12 RNAi Rep-3

text: ArrayExpress

text: L4 larva

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: WT cdk-12 RNAi Rep-3


E-MTAB-11189:WT cdk-12 RNAi Rep-4

characteristic: INSDC center name

text: public

text: E-MTAB-11189:WT cdk-12 RNAi Rep-4

text: ArrayExpress

text: L4 larva

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: hermaphrodite

text: WT cdk-12 RNAi Rep-4

characteristic: title


E-MTAB-11189:WT control RNAi Rep-3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11189:WT control RNAi Rep-3

text: ArrayExpress

text: L4 larva

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

text: WT control RNAi Rep-3


E-MTAB-11189:WT control RNAi Rep-4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11189:WT control RNAi Rep-4

characteristic: Submitter Id

text: ArrayExpress

text: L4 larva

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

text: WT control RNAi Rep-4


E-MTAB-11193:Sample 1

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11193:Sample 1

tag: Namespace:Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Venous blood was collected in EDTA Vacutainers (BD). Blood samples were diluted two times in PBS with 2 mM EDTA and layered onto Ficoll (Paneco) and then centrifuged at 300 g for 30 minutes at room temperature. Cell were separated with fluorescence activated cell sorting (BD FacsAria III, BD Biosciences) into the following subpopulations: memory B-cells (Bmem, CD19+ CD20+ CD27+ CD138-), plasmablasts (PBL, CD20- CD19+ CD27++ CD138-), plasma cells (PL, CD20- CD19 Low/- CD27+ CD138+) individually for each donor directly into RLT buffer (Qiagen, Germany). RNA extraction was performed using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s protocol Immunoglobulin heavy chain (IGH) cDNA libraries were prepared according to the protocol described earlier (Turchaninova et al. 2016) with several modifications. Briefly, we used 5'-RACE (Rapid Amplification of cDNA Ends) approach based on a template-switch effect to introduce 5’ adaptors during cDNA synthesis. These adaptors contain both unique molecular identifiers (UMIs), allowing error-correction, and sample barcodes (see Zvyagin et al. 2017 for the adaptor structure) for ruling out cross-sample contaminations. Universal sequence for annealing of forward PCR primer was also introduced by the adaptor. The set of primers specific for C-segment was used in an multiplex RT reaction followed by PCR amplification step with another semi-nested set of C-segment specific primers preserving isotype information.

characteristic: description

text: adult

text: normal

characteristic: disease

text: AT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: time

text: Sample 1