E-MTAB-11193:Sample 16

characteristic: External Id

text: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 16

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 16

characteristic: title


E-MTAB-11193:Sample 17

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 17

tag: Namespace:Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Venous blood was collected in EDTA Vacutainers (BD). Blood samples were diluted two times in PBS with 2 mM EDTA and layered onto Ficoll (Paneco) and then centrifuged at 300 g for 30 minutes at room temperature. Cell were separated with fluorescence activated cell sorting (BD FacsAria III, BD Biosciences) into the following subpopulations: memory B-cells (Bmem, CD19+ CD20+ CD27+ CD138-), plasmablasts (PBL, CD20- CD19+ CD27++ CD138-), plasma cells (PL, CD20- CD19 Low/- CD27+ CD138+) individually for each donor directly into RLT buffer (Qiagen, Germany). RNA extraction was performed using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s protocol Immunoglobulin heavy chain (IGH) cDNA libraries were prepared according to the protocol described earlier (Turchaninova et al. 2016) with several modifications. Briefly, we used 5'-RACE (Rapid Amplification of cDNA Ends) approach based on a template-switch effect to introduce 5’ adaptors during cDNA synthesis. These adaptors contain both unique molecular identifiers (UMIs), allowing error-correction, and sample barcodes (see Zvyagin et al. 2017 for the adaptor structure) for ruling out cross-sample contaminations. Universal sequence for annealing of forward PCR primer was also introduced by the adaptor. The set of primers specific for C-segment was used in an multiplex RT reaction followed by PCR amplification step with another semi-nested set of C-segment specific primers preserving isotype information.

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 17


E-MTAB-11193:Sample 22

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 22

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 22

characteristic: title


E-MTAB-11193:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 23

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 23


E-MTAB-11193:Sample 31

text: public

text: E-MTAB-11193:Sample 31

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IM

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 31


E-MTAB-11193:Sample 34

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 34

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 34

characteristic: title


E-MTAB-11193:Sample 35

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 35

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: IM

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 35


E-MTAB-11193:Sample 39

text: public

text: E-MTAB-11193:Sample 39

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IM

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 39


E-MTAB-11193:Sample 4

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 4

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 4

characteristic: title


E-MTAB-11193:Sample 40

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 40

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: IM

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 40


E-MTAB-11193:Sample 45

text: public

text: E-MTAB-11193:Sample 45

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IZ

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 45


E-MTAB-11193:Sample 48

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 48

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IZ

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 48

characteristic: title


E-MTAB-11193:Sample 49

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 49

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: IZ

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 49


E-MTAB-11193:Sample 5

text: public

text: E-MTAB-11193:Sample 5

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: AT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 5


E-MTAB-11193:Sample 54

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 54

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IZ

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 54

characteristic: title


E-MTAB-11193:Sample 55

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 55

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: IZ

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 55


E-MTAB-11193:Sample 60

text: public

text: E-MTAB-11193:Sample 60

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 60


E-MTAB-11193:Sample 63

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 63

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 63

characteristic: title


E-MTAB-11193:Sample 64

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 64

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: MRK

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 64


E-MTAB-11193:Sample 71

text: public

text: E-MTAB-11193:Sample 71

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 71


E-MTAB-11193:Sample 72

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 72

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 72

characteristic: title


E-MTAB-11193:Sample 77

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 77

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: MT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 77


E-MTAB-11193:Sample 78

text: public

text: E-MTAB-11193:Sample 78

text: ArrayExpress

text: memory B cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 78


E-MTAB-11193:Sample 8

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 8

text: ArrayExpress

text: memory B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 8

characteristic: title


E-MTAB-11193:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 9

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: memory B cell

text: human

text: adult

text: normal

text: AT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 9


E-MTAB-11193:Sample 29

text: public

text: E-MTAB-11193:Sample 29

text: ArrayExpress

text: mononuclear cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 29


E-MTAB-11193:Sample 30

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 30

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: MT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 30

characteristic: title


E-MTAB-11193:Sample 82

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 82

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 82


E-MTAB-11193:Sample 83

text: public

text: E-MTAB-11193:Sample 83

text: ArrayExpress

text: mononuclear cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 83


E-MTAB-11193:Sample 12

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 12

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 12

characteristic: title


E-MTAB-11193:Sample 13

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 13

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: AT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 13


E-MTAB-11193:Sample 20

text: public

text: E-MTAB-11193:Sample 20

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 20


E-MTAB-11193:Sample 21

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 21

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 21

characteristic: title


E-MTAB-11193:Sample 27

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 27

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 27


E-MTAB-11193:Sample 28

text: public

text: E-MTAB-11193:Sample 28

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 28


E-MTAB-11193:Sample 3

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 3

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 3

characteristic: title


E-MTAB-11193:Sample 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 33

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: IM

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 33


E-MTAB-11193:Sample 38

text: public

text: E-MTAB-11193:Sample 38

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IM

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 38


E-MTAB-11193:Sample 43

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 43

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 43

characteristic: title


E-MTAB-11193:Sample 44

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 44

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: IM

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 44


E-MTAB-11193:Sample 47

text: public

text: E-MTAB-11193:Sample 47

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IZ

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 47


E-MTAB-11193:Sample 52

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 52

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IZ

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 52

characteristic: title


E-MTAB-11193:Sample 53

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 53

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: IZ

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 53


E-MTAB-11193:Sample 58

text: public

text: E-MTAB-11193:Sample 58

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IZ

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 58


E-MTAB-11193:Sample 59

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 59

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: IZ

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 59

characteristic: title


E-MTAB-11193:Sample 62

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 62

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: MRK

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 62


E-MTAB-11193:Sample 68

text: public

text: E-MTAB-11193:Sample 68

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 68


E-MTAB-11193:Sample 69

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 69

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 69

characteristic: title


E-MTAB-11193:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 7

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: AT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 7


E-MTAB-11193:Sample 70

text: public

text: E-MTAB-11193:Sample 70

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 70


E-MTAB-11193:Sample 75

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 75

text: ArrayExpress

text: plasma cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 75

characteristic: title


E-MTAB-11193:Sample 76

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 76

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasma cell

text: human

text: adult

text: normal

text: MRK

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 76


E-MTAB-11193:Sample 81

text: public

text: E-MTAB-11193:Sample 81

text: ArrayExpress

text: plasma cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 81


E-MTAB-11193:Sample 10

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 10

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 10

characteristic: title


E-MTAB-11193:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 11

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: AT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 11


E-MTAB-11193:Sample 18

text: public

text: E-MTAB-11193:Sample 18

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 18


E-MTAB-11193:Sample 19

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 19

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 19

characteristic: title


E-MTAB-11193:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: AT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 2


E-MTAB-11193:Sample 24

text: public

text: E-MTAB-11193:Sample 24

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 24


E-MTAB-11193:Sample 25

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 25

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 25

characteristic: title


E-MTAB-11193:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 26

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 26


E-MTAB-11193:Sample 32

text: public

text: E-MTAB-11193:Sample 32

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IM

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 32


E-MTAB-11193:Sample 36

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 36

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: IM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 36

characteristic: title


E-MTAB-11193:Sample 37

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 37

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: IM

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 37


E-MTAB-11193:Sample 41

text: public

text: E-MTAB-11193:Sample 41

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IM

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 41


E-MTAB-11193:Sample 42

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 42

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: IM

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 42

characteristic: title


E-MTAB-11193:Sample 46

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 46

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: IZ

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 46


E-MTAB-11193:Sample 50

text: public

text: E-MTAB-11193:Sample 50

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IZ

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 50


E-MTAB-11193:Sample 51

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 51

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: IZ

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 51

characteristic: title


E-MTAB-11193:Sample 56

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 56

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: IZ

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 56


E-MTAB-11193:Sample 57

text: public

text: E-MTAB-11193:Sample 57

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: IZ

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 57


E-MTAB-11193:Sample 6

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 6

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: AT

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 6

characteristic: title


E-MTAB-11193:Sample 61

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 61

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: MRK

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 61


E-MTAB-11193:Sample 65

text: public

text: E-MTAB-11193:Sample 65

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 65


E-MTAB-11193:Sample 66

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 66

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 66

characteristic: title


E-MTAB-11193:Sample 67

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 67

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: MRK

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Sample 67


E-MTAB-11193:Sample 73

text: public

text: E-MTAB-11193:Sample 73

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MRK

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 73


E-MTAB-11193:Sample 74

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 74

text: ArrayExpress

text: plasmablast

text: human

text: adult

characteristic: developmental stage

text: normal

text: MRK

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 74

characteristic: title


E-MTAB-11193:Sample 79

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 79

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: plasmablast

text: human

text: adult

text: normal

text: MT

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 79


E-MTAB-11193:Sample 80

text: public

text: E-MTAB-11193:Sample 80

text: ArrayExpress

text: plasmablast

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: MT

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

characteristic: replicate label

text: Homo sapiens

text: female

characteristic: sex

characteristic: time

text: Sample 80


E-MTAB-11193:Sample 14

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11193:Sample 14

text: ArrayExpress

text: B cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 14

characteristic: title


E-MTAB-11193:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11193:Sample 15

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Sample 15


text: NIPH

text: public

text: ELIXIR-Norway

characteristic: collection date

text: S. pyogenes

characteristic: common name

text: GAS routine sample

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: synovial fluid

characteristic: isolation source

text: Streptococcus pyogenes

text: Streptococcus pyogenes


characteristic: External Id

text: NIPH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

text: Blood

text: Streptococcus pyogenes

characteristic: organism

text: Streptococcus pyogenes

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NIPH

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

text: vaginal secretion

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: public

tag: Namespace:University of Helsinki TILT Biotherapeutics

text: 4 to 6

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Tumours were mechanically disrupted using gentleMACs C tubes (Milltenyi Biotech) followed by enzymatic digestion using collagenase type IV (1mg/mL), Hyaluronidase (2U/mL) and DNase I (10U/mL) in RPMI with 10% FBS for 1 hour. After double filtering of disrupted tumor tissue, CD45 Microbeads, mouse ( 130-052-301 Miltenyi Biotec) were used to isolate +/- fractions which were than placed into RNA later for isolation. 4–6 weeks old female C57BL/6JOlaHsd (immunocompetent) mice were purchased from Envigo (Indianapolis, IN, USA). Mice were subcutaneously (right flank) injected with either 2x106 MOC1 cells in 50μl PBS. Established tumours (4mm)(stratified randomization) were treated with anti-PD-L1 Ab (clone 10 F.9G2, BioXcell) (100 µg/injection). When tumours reached 8mm (refractory), they were than treated with either intratumoral injection of PBS, 1 × 109 VP of unarmed control virus (Ad5-Luc), 1:1 mixture of Ad5-CMV-mTNFα and Ad5-CMV-mIL2 virus (total 1 × 109 VP) or anti-PD-L1 Ab (clone 10 F.9G2, BioXcell) (100 µg/injection). After extraction of gene hit counts, the gene hit counts table was used for downstream differential expression analysis. Using DESeq2, a comparison of gene expression between the customer-defined groups of samples was performed. The Wald test was used to generate p-values and log2 fold changes. Genes with an adjusted p-value < 0.05 and absolute log2 fold change > 1 were called as differentially expressed genes for each comparison. Below are the results of the number of significantly differentially expressed genes for all comparisons provided. RNA from CD45+/- MOC1 tumour cells was purified using RNeasy kit according to the manufacturer’s instructions (74106, Qiagen, Hilden, Germany) PolyA selection

characteristic: description

text: oral squamous cell carcinoma

characteristic: disease

text: in vivo until neoplasm formation and refractory period

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: oral cavity

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain


characteristic: External Id

text: University of Helsinki TILT Biotherapeutics

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 4 to 6

text: ArrayExpress

text: house mouse

text: oral squamous cell carcinoma

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: oral cavity

text: Mus musculus

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Latvian Biomedical Research and Study Centre

text: Strain A, start phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus

characteristic: title


text: Latvian Biomedical Research and Study Centre

text: public

characteristic: Submitter Id

text: Strain B, start phase, 0mM

characteristic: description

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, start phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, initiation phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, end phase, 0mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, medium phase, 40mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain C, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain A, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: public

text: Strain B, medium phase, 100mM

text: Kluyveromyces marxianus

text: Kluyveromyces marxianus


text: OXFORD

text: public

text: 10 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Livers were harvested at ZT18 and immediately frozen before storage at -80. Samples were fixed in 4% formaldehyde for 10 minutes immediately upon thawing, before isolation of nuclei and immunoprecipitation specific histone-DNA complexes. Nucleic acid was extracted by phenol-chloroform and fragmented by sonication. ChIP-seq libraries were generated from the ChIP- and Input-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara).

text: adult

characteristic: developmental stage

text: mixed MLL3 delta mutation and wild type genotype

characteristic: genotype

text: mixed pool of all mice

characteristic: individual

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: mixed

text: C57BL/6 x 129 mouse strain

text: Input


characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 10 to 14

text: ArrayExpress

text: house mouse

text: adult

text: MLL3 delta mutation

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: C57BL/6 x 129 mouse strain

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: OXFORD

text: public

characteristic: INSDC status

text: 10 to 14

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: Mus musculus

text: male

characteristic: sex

text: C57BL/6 x 129 mouse strain


text: OXFORD

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 10 to 14

characteristic: age

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: female

text: C57BL/6 x 129 mouse strain

characteristic: title


characteristic: External Id

text: OXFORD

text: public

text: 10 to 14

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: Mus musculus

text: C57BL/6 x 129 mouse strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 10 to 14

text: ArrayExpress

text: house mouse

text: adult

text: MLL3 delta mutation

text: Mus musculus

text: Mus musculus

text: liver

text: Mus musculus

text: male

text: C57BL/6 x 129 mouse strain

characteristic: strain


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF EXETER

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF EXETER

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

unit: DD

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

unit: cm

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

text: 98 �C for 30 seconds; 30 cycles of 98 �C for 10 seconds, 55 �C for 30 seconds, 72 �C for 30 seconds; and a final extension of 72 �C for 2 minutes

characteristic: pcr conditions

text: 515F (5'-GTGYCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT-3')

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 1 Pond 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapi 2 Pond 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 1 Pond 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 1 Pond 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 2 Pond 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 3 Pond 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 4 Pond 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 5 Pond 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Banded tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 1 Pond 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 6 Pond 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Banded tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 2 Pond 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 7 Pond 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Banded tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 3 Pond 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 4 Pond 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 5 Pond 4


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 6 Pond 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 1 Pond 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 7 Pond 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 2 Pond 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 8 Pond 4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 3 Pond 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Blantyre

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Redbreast tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 4 Pond 5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Blantyre

text: skin mucous

text: fish skin

text: Redbreast tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 5 Pond 5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Maldeco, Mangochi

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 1 Pond 6

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Maldeco, Mangochi

text: skin mucous

text: fish skin

text: Tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 2 Pond 6


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Maldeco, Mangochi

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 3 Pond 6

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Maldeco, Mangochi

text: skin mucous

text: fish skin

text: Tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 4 Pond 6


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Maldeco, Mangochi

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 5 Pond 6

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Maldeco, Mangochi

text: skin mucous

text: fish skin

text: Tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 1 Pond 7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Maldeco, Mangochi

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 2 Pond 7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Tilapia fish skin swab

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: skin environment (ENVO:2100003)

text: Malawi

text: not collected

text: not collected

text: Maldeco, Mangochi

text: skin mucous

text: fish skin

text: Tilapia

text: omnivorous

characteristic: host diet

text: healthy

characteristic: host disease status

characteristic: host length

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: fish metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: swab

characteristic: sample collection device or method

text: stored in RNAlater

characteristic: sample material processing

text: fish metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Tilapia 3 Pond 7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Tilapia fish skin swab

characteristic: description

text: freshwater biome (ENVO:00000873)

characteristic: environment (biome)

text: pond (ENVO:00000033)

characteristic: environment (feature)

text: skin environment (ENVO:2100003)

characteristic: environment (material)

text: Malawi

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Maldeco, Mangochi

characteristic: geographic location (region and locality)

text: skin mucous

characteristic: host body product

text: fish skin

characteristic: host body site

text: Tilapia

characteristic: host common name

text: omnivorous

text: healthy

text: host-associated

text: mimarks-survey

text: fish metagenome

text: Tilapia skin microbiomes Malawi

text: swab

text: stored in RNAlater

text: fish metagenome

text: Illumina MiSeq

text: 16S rRNA

text: Tilapia 4 Pond 7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF EXETER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

unit: L

characteristic: amount or size of sample collected

text: Pond water filter from tilapia aquaculture

text: freshwater biome (ENVO:00000873)

text: pond (ENVO:00000033)

text: fresh water (ENVO:00002011)

text: Malawi

unit: m

characteristic: geographic location (depth)

text: not collected

text: not collected

text: Blantyre

text: mimarks-survey

characteristic: investigation type

characteristic: nucleic acid extraction

text: pond metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: Tilapia skin microbiomes Malawi

characteristic: project name

text: 0.4 um polycarbonate filter

characteristic: sample collection device or method

text: stored in ethanol

characteristic: sample material processing

text: pond metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: Pond 1 replica a

text: water

characteristic: water environmental package