characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i705_i510.H3FT3DRXY_SLX.20307.i705_i510.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i706_i502.H3FT3DRXY_SLX.20307.i706_i502.H3MLCDRXY

tag: Namespace:UNIVERSITY OF CAMBRIDGE

characteristic: Submitter Id

unit: day

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i706_i505.H3FT3DRXY_SLX.20307.i706_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i706_i506.H3FT3DRXY_SLX.20307.i706_i506.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i706_i507.H3FT3DRXY_SLX.20307.i706_i507.H3MLCDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i706_i508.H3FT3DRXY_SLX.20307.i706_i508.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: trophoblast

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: trophoblast

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i706_i510.H3FT3DRXY_SLX.20307.i706_i510.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i707_i502.H3FT3DRXY_SLX.20307.i707_i502.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i707_i503.H3FT3DRXY_SLX.20307.i707_i503.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i707_i505.H3FT3DRXY_SLX.20307.i707_i505.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i707_i506.H3FT3DRXY_SLX.20307.i707_i506.H3MLCDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i707_i507.H3FT3DRXY_SLX.20307.i707_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i707_i508.H3FT3DRXY_SLX.20307.i707_i508.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i710_i503.H3FT3DRXY_SLX.20307.i710_i503.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i710_i505.H3FT3DRXY_SLX.20307.i710_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i710_i507.H3FT3DRXY_SLX.20307.i710_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i710_i508.H3FT3DRXY_SLX.20307.i710_i508.H3MLCDRXY

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: amnion

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i710_i510.H3FT3DRXY_SLX.20307.i710_i510.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i711_i503.H3FT3DRXY_SLX.20307.i711_i503.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i711_i505.H3FT3DRXY_SLX.20307.i711_i505.H3MLCDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i711_i507.H3FT3DRXY_SLX.20307.i711_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i712_i503.H3FT3DRXY_SLX.20307.i712_i503.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i712_i505.H3FT3DRXY_SLX.20307.i712_i505.H3MLCDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i712_i510.H3FT3DRXY_SLX.20307.i712_i510.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i714_i507.H3FT3DRXY_SLX.20307.i714_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i714_i510.H3FT3DRXY_SLX.20307.i714_i510.H3MLCDRXY

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: amnion

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i715_i503.H3FT3DRXY_SLX.20307.i715_i503.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i715_i505.H3FT3DRXY_SLX.20307.i715_i505.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20307.i715_i507.H3FT3DRXY_SLX.20307.i715_i507.H3MLCDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20307.i715_i508.H3FT3DRXY_SLX.20307.i715_i508.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20307.i715_i511.H3FT3DRXY_SLX.20307.i715_i511.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i701_i502.H3FT3DRXY_SLX.20308.i701_i502.H3MLCDRXY

text: ArrayExpress

text: trophoblast

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: trophoblast

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i701_i503.H3FT3DRXY_SLX.20308.i701_i503.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i701_i505.H3FT3DRXY_SLX.20308.i701_i505.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i701_i506.H3FT3DRXY_SLX.20308.i701_i506.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i701_i507.H3FT3DRXY_SLX.20308.i701_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i701_i508.H3FT3DRXY_SLX.20308.i701_i508.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i701_i511.H3FT3DRXY_SLX.20308.i701_i511.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i702_i502.H3FT3DRXY_SLX.20308.i702_i502.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: trophoblast

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: trophoblast

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i702_i503.H3FT3DRXY_SLX.20308.i702_i503.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i702_i505.H3FT3DRXY_SLX.20308.i702_i505.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i702_i506.H3FT3DRXY_SLX.20308.i702_i506.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i702_i507.H3FT3DRXY_SLX.20308.i702_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i702_i511.H3FT3DRXY_SLX.20308.i702_i511.H3MLCDRXY

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i703_i502.H3FT3DRXY_SLX.20308.i703_i502.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i703_i503.H3FT3DRXY_SLX.20308.i703_i503.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i703_i505.H3FT3DRXY_SLX.20308.i703_i505.H3MLCDRXY

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: secondary yolk sac

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i703_i506.H3FT3DRXY_SLX.20308.i703_i506.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i703_i507.H3FT3DRXY_SLX.20308.i703_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i703_i508.H3FT3DRXY_SLX.20308.i703_i508.H3MLCDRXY

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: amnion

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i703_i511.H3FT3DRXY_SLX.20308.i703_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i704_i502.H3FT3DRXY_SLX.20308.i704_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i704_i503.H3FT3DRXY_SLX.20308.i704_i503.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i704_i505.H3FT3DRXY_SLX.20308.i704_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i704_i506.H3FT3DRXY_SLX.20308.i704_i506.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i704_i507.H3FT3DRXY_SLX.20308.i704_i507.H3MLCDRXY

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i704_i510.H3FT3DRXY_SLX.20308.i704_i510.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i704_i511.H3FT3DRXY_SLX.20308.i704_i511.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i705_i502.H3FT3DRXY_SLX.20308.i705_i502.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i705_i503.H3FT3DRXY_SLX.20308.i705_i503.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i705_i506.H3FT3DRXY_SLX.20308.i705_i506.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i705_i507.H3FT3DRXY_SLX.20308.i705_i507.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i705_i511.H3FT3DRXY_SLX.20308.i705_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i706_i502.H3FT3DRXY_SLX.20308.i706_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i706_i506.H3FT3DRXY_SLX.20308.i706_i506.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i706_i507.H3FT3DRXY_SLX.20308.i706_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i706_i510.H3FT3DRXY_SLX.20308.i706_i510.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i706_i511.H3FT3DRXY_SLX.20308.i706_i511.H3MLCDRXY

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i707_i502.H3FT3DRXY_SLX.20308.i707_i502.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i707_i503.H3FT3DRXY_SLX.20308.i707_i503.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i707_i506.H3FT3DRXY_SLX.20308.i707_i506.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i707_i507.H3FT3DRXY_SLX.20308.i707_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i707_i508.H3FT3DRXY_SLX.20308.i707_i508.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i707_i510.H3FT3DRXY_SLX.20308.i707_i510.H3MLCDRXY

text: ArrayExpress

text: trophoblast

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: trophoblast

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i707_i511.H3FT3DRXY_SLX.20308.i707_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i710_i502.H3FT3DRXY_SLX.20308.i710_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i710_i503.H3FT3DRXY_SLX.20308.i710_i503.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i710_i506.H3FT3DRXY_SLX.20308.i710_i506.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i710_i507.H3FT3DRXY_SLX.20308.i710_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i710_i510.H3FT3DRXY_SLX.20308.i710_i510.H3MLCDRXY

text: ArrayExpress

text: remodelled endometrium

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 5-7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: remodelled endometrium

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i710_i511.H3FT3DRXY_SLX.20308.i710_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i711_i502.H3FT3DRXY_SLX.20308.i711_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i711_i503.H3FT3DRXY_SLX.20308.i711_i503.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i711_i505.H3FT3DRXY_SLX.20308.i711_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i711_i507.H3FT3DRXY_SLX.20308.i711_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i711_i510.H3FT3DRXY_SLX.20308.i711_i510.H3MLCDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i711_i511.H3FT3DRXY_SLX.20308.i711_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i712_i502.H3FT3DRXY_SLX.20308.i712_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i712_i503.H3FT3DRXY_SLX.20308.i712_i503.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i712_i507.H3FT3DRXY_SLX.20308.i712_i507.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i712_i508.H3FT3DRXY_SLX.20308.i712_i508.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i712_i510.H3FT3DRXY_SLX.20308.i712_i510.H3MLCDRXY

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: secondary yolk sac

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i712_i511.H3FT3DRXY_SLX.20308.i712_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i714_i502.H3FT3DRXY_SLX.20308.i714_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i714_i503.H3FT3DRXY_SLX.20308.i714_i503.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i714_i505.H3FT3DRXY_SLX.20308.i714_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i714_i507.H3FT3DRXY_SLX.20308.i714_i507.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i714_i510.H3FT3DRXY_SLX.20308.i714_i510.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i714_i511.H3FT3DRXY_SLX.20308.i714_i511.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i715_i502.H3FT3DRXY_SLX.20308.i715_i502.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i715_i503.H3FT3DRXY_SLX.20308.i715_i503.H3MLCDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i715_i505.H3FT3DRXY_SLX.20308.i715_i505.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i715_i506.H3FT3DRXY_SLX.20308.i715_i506.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20308.i715_i507.H3FT3DRXY_SLX.20308.i715_i507.H3MLCDRXY

text: ArrayExpress

text: stalk

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: stalk

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20308.i715_i510.H3FT3DRXY_SLX.20308.i715_i510.H3MLCDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stalk

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stalk

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20308.i715_i511.H3FT3DRXY_SLX.20308.i715_i511.H3MLCDRXY

characteristic: Submitter Id

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i701_i502.H3NYWDRXY

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i701_i503.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i701_i506.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: primordial germ cell

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i701_i507.H3NYWDRXY

text: ArrayExpress

text: primordial germ cell

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: primordial germ cell

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i701_i508.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stroma

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 5-7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: stroma

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i701_i510.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i702_i502.H3NYWDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i702_i503.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i702_i505.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i702_i506.H3NYWDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i702_i507.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i702_i510.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i703_i502.H3NYWDRXY

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: amnion

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i703_i503.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i703_i505.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i703_i508.H3NYWDRXY

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: extraembryonic mesoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i703_i511.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i704_i502.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i704_i507.H3NYWDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i704_i508.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i705_i502.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: visceral endoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i705_i503.H3NYWDRXY

text: ArrayExpress

text: visceral endoderm

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: visceral endoderm

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i705_i505.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i705_i507.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i705_i508.H3NYWDRXY

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: secondary yolk sac

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i705_i510.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i705_i511.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: extraembryonic mesoderm

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: extraembryonic mesoderm

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i706_i502.H3NYWDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i706_i505.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 7

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i706_i507.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 7

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: secondary yolk sac

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i706_i508.H3NYWDRXY

text: ArrayExpress

text: secondary yolk sac

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 6

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: secondary yolk sac

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC center name

text: public

text: E-MTAB-9367_2:SLX.20329.i706_i510.H3NYWDRXY

characteristic: age

text: ArrayExpress

characteristic: broker name

text: embryonic disc

text: white-tufted-ear marmoset

characteristic: common name

text: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).

text: embryo stage

characteristic: developmental stage

text: Carnegie Stage 6

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: embryonic disc

text: pass

text: Callithrix jacchus

text: not available

text: OK


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-9367_2:SLX.20329.i706_i511.H3NYWDRXY

characteristic: Submitter Id

text: ArrayExpress

text: amnion

text: white-tufted-ear marmoset

characteristic: description

text: embryo stage

text: Carnegie Stage 6

characteristic: embryo stage

text: not applicable

text: Callithrix jacchus

text: Callithrix jacchus

text: amnion

text: pass

text: Callithrix jacchus

text: not available

text: OK


text: UNIVERSITY OF CAMBRIDGE

text: public

text: E-MTAB-9367_2:SLX.20329.i707_i502.H3NYWDRXY

text: ArrayExpress

text: embryonic disc

text: white-tufted-ear marmoset

text: embryo stage

text: Carnegie Stage 7

characteristic: individual

text: not applicable

characteristic: isolate

text: Callithrix jacchus

characteristic: organism

text: Callithrix jacchus

text: embryonic disc

characteristic: organism part

text: pass

characteristic: post analysis well quality

text: Callithrix jacchus

text: not available

characteristic: sex

text: OK

characteristic: single cell well quality

characteristic: title