characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Roseburia;s__Roseburia hominis

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738352) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567292) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_F_9_RAU0028AZ.14

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7738352 were assembled into contigs (ERZ4567292). The following read sets from this study were the most similar to ERR7738352 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738352,ERR7738662,ERR7738583,ERR7745760,ERR7738265,ERR7738653,ERR7738619,ERR7738533,ERR7745908,ERR7745769. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567292. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: f__Lachnospiraceae (UID1286)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738352 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Roseburia sp. MAG assembled and binned from ERR7738352.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Nepal

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Roseburia sp.

characteristic: organism

text: Metagenome sequencing of Nepali gut microbiome

text: No

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

text: GTDB-TK v1.7.0, r202 database, default parameters

text: multi-marker approach


status: PUBLIC

tag: attribute

characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745922) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559426) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_O_10_2644.179

tag: Namespace:Stanford University School of Medicine

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7745922 were assembled into contigs (ERZ4559426). The following read sets from this study were the most similar to ERR7745922 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745922,ERR7745948,ERR7746344,ERR7737973,ERR7738172,ERR7745675,ERR7745384,ERR7745389,ERR7738210,ERR7738182. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559426. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7745922 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Roseburia sp. MAG assembled and binned from ERR7745922.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

text: uncultured Roseburia sp.

text: Metagenome sequencing of Hadza gut microbiome

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Roseburia;s__Roseburia hominis

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745291) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559329) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_O_11_2507.252

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7745291 were assembled into contigs (ERZ4559329). The following read sets from this study were the most similar to ERR7745291 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745291,ERR7745783,ERR7738569,ERR7738604,ERR7738461,ERR7746321,ERR7746365,ERR7746398,ERR7738605,ERR7747782. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559329. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7745291 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Roseburia sp. MAG assembled and binned from ERR7745291.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Roseburia sp.

characteristic: organism

text: No

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7746219) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559398) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_M_10_2632.93

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7746219 were assembled into contigs (ERZ4559398). The following read sets from this study were the most similar to ERR7746219 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746219,ERR7745990,ERR7747255,ERR7745845,ERR7746094,ERR7738588,ERR7745759,ERR7746767,ERR7746440,ERR7745588. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559398. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: f__Lachnospiraceae (UID1286)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7746219 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Roseburia sp. MAG assembled and binned from ERR7746219.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Roseburia sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738216) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559673) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_N_16_2338.76

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738216 were assembled into contigs (ERZ4559673). The following read sets from this study were the most similar to ERR7738216 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738216,ERR7738203,ERR7738569,ERR7738235,ERR7745420,ERR7738234,ERR7745427,ERR7738189,ERR7738157,ERR7738182. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559673. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738216 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Roseburia sp. MAG assembled and binned from ERR7738216.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Roseburia sp.

characteristic: organism

text: No

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

text: GTDB-TK v1.7.0, r202 database, default parameters

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__UBA6398;s__UBA6398 sp003150315

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738592) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567471) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_B_21_THA1063YZ.28

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738592 were assembled into contigs (ERZ4567471). The following read sets from this study were the most similar to ERR7738592 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738592,ERR7738572,ERR7738472,ERR7738558,ERR7738529,ERR7745779,ERR7745858,ERR7738619,ERR7738575,ERR7745769. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567471. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: o__Bacteroidales (UID2657)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738592 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Bacteroidaceae bacterium MAG assembled and binned from ERR7738592.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Nepal

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Bacteroidaceae bacterium

text: Metagenome sequencing of Nepali gut microbiome

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Bacteroidaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Eisenbergiella;s__Eisenbergiella sp900066775

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7737964) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561898) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_K_19_1040.22

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7737964 were assembled into contigs (ERZ4561898). The following read sets from this study were the most similar to ERR7737964 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7737964,ERR7738579,ERR7738250,ERR7738527,ERR7738577,ERR7746398,ERR7738154,ERR7745903,ERR7738244,ERR7738613. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561898. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: f__Lachnospiraceae (UID1255)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7737964 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Eisenbergiella sp. MAG assembled and binned from ERR7737964.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: number of standard tRNAs extracted

text: uncultured Eisenbergiella sp.

characteristic: organism

text: Metagenome sequencing of Hadza gut microbiome

text: No

text: uncultured Eisenbergiella sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738245) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561113) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_B_21_1589.67

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738245 were assembled into contigs (ERZ4561113). The following read sets from this study were the most similar to ERR7738245 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738245,ERR7745434,ERR7738188,ERR7746184,ERR7738565,ERR7745919,ERR7738556,ERR7738651,ERR7738180,ERR7738531. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561113. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738245 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Eisenbergiella sp. MAG assembled and binned from ERR7738245.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Eisenbergiella sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Eisenbergiella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

text: GTDB-TK v1.7.0, r202 database, default parameters

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__TF01-11;s__TF01-11 sp003529475

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738665) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4558928) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_SUBJECT_CONTIGS_1500_ASSEMBLY_K77_MERGED__Pilot_MoBio_Fiber_I_11_1003.61

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738665 were assembled into contigs (ERZ4558928). The following read sets from this study were the most similar to ERR7738665 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738665,ERR7738546. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 2 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4558928. Depth information for all 2 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: o__Clostridiales (UID1212)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738665 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738665.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Metagenome sequencing of Californian gut microbiome

text: No

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__GCA-900066135;s__GCA-900066135 sp900066135

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738660) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567818) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_A_8_1525.200

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7738660 were assembled into contigs (ERZ4567818). The following read sets from this study were the most similar to ERR7738660 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738660,ERR7738561,ERR7738251,ERR7746660,ERR7738614,ERR7738586,ERR7738228,ERR7738275,ERR7746321,ERR7738219. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567818. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738660 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738660.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Lachnospiraceae bacterium

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__GCA-900066135;s__GCA-900066135 sp900543575

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738633) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4558881) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_SUBJECT_CONTIGS_1500_ASSEMBLY_K77_MERGED__Pilot_MoBio_Fiber-Hadza-Nepal_M_3_7038.18

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738633 were assembled into contigs (ERZ4558881). The following read sets from this study were the most similar to ERR7738633 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738633,ERR7738620. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 2 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4558881. Depth information for all 2 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738633 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738633.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: No

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: GTDB-TK v1.7.0, r202 database, default parameters

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__UMGS1375;s__

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738587) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567913) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_A_20_1653.263

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738587 were assembled into contigs (ERZ4567913). The following read sets from this study were the most similar to ERR7738587 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738587,ERR7738628,ERR7738602,ERR7738531,ERR7738565,ERR7738557,ERR7745963,ERR7746321,ERR7745924,ERR7746349. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567913. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738587 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738587.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

text: uncultured Lachnospiraceae bacterium

text: Metagenome sequencing of Hadza gut microbiome

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__UMGS1375;s__UMGS1375 sp900066615

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738569) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567755) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_C_10_2277.178

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7738569 were assembled into contigs (ERZ4567755). The following read sets from this study were the most similar to ERR7738569 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738569,ERR7738203,ERR7747426,ERR7738608,ERR7745815,ERR7738461,ERR7746365,ERR7746184,ERR7738610,ERR7738200. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567755. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738569 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738569.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: No

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Ruminococcus_A;s__

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7746048) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561940) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_G_7_1734.107

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7746048 were assembled into contigs (ERZ4561940). The following read sets from this study were the most similar to ERR7746048 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746048,ERR7738613,ERR7738577,ERR7738936,ERR7738569,ERR7738563,ERR7747394,ERR7738595,ERR7738599,ERR7746321. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561940. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7746048 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Ruminococcus sp. MAG assembled and binned from ERR7746048.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Ruminococcus sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

text: GTDB-TK v1.7.0, r202 database, default parameters

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745559) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4560875) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_L_3_1539.247

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7745559 were assembled into contigs (ERZ4560875). The following read sets from this study were the most similar to ERR7745559 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745559,ERR7738222,ERR7745438,ERR7746793,ERR7745953,ERR7738187,ERR7747522,ERR7746061,ERR7745975,ERR7738264. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4560875. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7745559 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Ruminococcus sp. MAG assembled and binned from ERR7745559.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: number of standard tRNAs extracted

text: uncultured Ruminococcus sp.

characteristic: organism

text: Metagenome sequencing of Hadza gut microbiome

text: No

text: uncultured Ruminococcus sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7746217) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567680) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_C_12_2317.323

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7746217 were assembled into contigs (ERZ4567680). The following read sets from this study were the most similar to ERR7746217 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746217,ERR7747128,ERR7738186,ERR7745947,ERR7738957,ERR7745854,ERR7746465,ERR7738606,ERR7746345,ERR7738534. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567680. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7746217 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Ruminococcus sp. MAG assembled and binned from ERR7746217.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Ruminococcus sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Ruminococcus_A;s__

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738614) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567908) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_I_10_1184.89

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738614 were assembled into contigs (ERZ4567908). The following read sets from this study were the most similar to ERR7738614 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738614,ERR7738219,ERR7738561,ERR7738660,ERR7738251,ERR7738559,ERR7745815,ERR7746321,ERR7738586,ERR7738242. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567908. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: k__Bacteria (UID203)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738614 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Ruminococcus sp. MAG assembled and binned from ERR7738614.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Ruminococcus sp.

characteristic: organism

text: No

text: uncultured Ruminococcus sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Ruminococcus_A;s__Ruminococcus_A faecicola

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745890) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4560264) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_F_18_2239.13

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7745890 were assembled into contigs (ERZ4560264). The following read sets from this study were the most similar to ERR7745890 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745890,ERR7738537,ERR7738197,ERR7747394,ERR7738552,ERR7747255,ERR7738624,ERR7745335,ERR7745859,ERR7738569. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4560264. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7745890 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Ruminococcus sp. MAG assembled and binned from ERR7745890.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

text: uncultured Ruminococcus sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

text: GTDB-TK v1.7.0, r202 database, default parameters

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__UBA6398;s__

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745329) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559348) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_G_24_2613.56

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7745329 were assembled into contigs (ERZ4559348). The following read sets from this study were the most similar to ERR7745329 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745329,ERR7746440,ERR7747125,ERR7745759,ERR7738271,ERR7747128,ERR7745845,ERR7738604,ERR7738594,ERR7746219. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559348. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: o__Bacteroidales (UID2657)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7745329 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Bacteroidaceae bacterium MAG assembled and binned from ERR7745329.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Bacteroidaceae bacterium

characteristic: organism

text: Metagenome sequencing of Hadza gut microbiome

text: No

text: uncultured Bacteroidaceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Fusicatenibacter;s__Fusicatenibacter saccharivorans

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738514) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567069) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_SUBJECT_CONTIGS_1500_ASSEMBLY_K77_MERGED__Pilot_MoBio_Fiber-Hadza-Nepal_C_23_7024.225

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738514 were assembled into contigs (ERZ4567069). The following read sets from this study were the most similar to ERR7738514 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738514,ERR7738547. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 2 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567069. Depth information for all 2 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738514 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7738514.

text: terrestrial biome

text: human-associated habitat

text: feces

text: USA

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Fusicatenibacter sp.

text: Metagenome sequencing of Californian gut microbiome

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738274) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559904) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_K_19_2488.256

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738274 were assembled into contigs (ERZ4559904). The following read sets from this study were the most similar to ERR7738274 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738274,ERR7745719,ERR7745867,ERR7745402,ERR7745410,ERR7745819,ERR7738165,ERR7738207,ERR7738604,ERR7738569. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559904. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7738274 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7738274.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: number of standard tRNAs extracted

text: uncultured Fusicatenibacter sp.

characteristic: organism

text: No

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

text: GTDB-TK v1.7.0, r202 database, default parameters

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738223) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4560427) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_A_8_1817.239

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7738223 were assembled into contigs (ERZ4560427). The following read sets from this study were the most similar to ERR7738223 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738223,ERR7747140,ERR7745994,ERR7738936,ERR7738598,ERR7738595,ERR7738607,ERR7738567,ERR7747324,ERR7745335. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4560427. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738223 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7738223.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Fusicatenibacter sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Fusicatenibacter;s__Fusicatenibacter saccharivorans

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7746349) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4568042) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_E_2_1315.78

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7746349 were assembled into contigs (ERZ4568042). The following read sets from this study were the most similar to ERR7746349 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746349,ERR7738602,ERR7738628,ERR7738571,ERR7738552,ERR7745963,ERR7738587,ERR7745544,ERR7738557,ERR7738584. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4568042. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7746349 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7746349.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Fusicatenibacter sp.

characteristic: organism

text: Metagenome sequencing of Hadza gut microbiome

text: No

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738586) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567843) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_E_6_1527.99

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738586 were assembled into contigs (ERZ4567843). The following read sets from this study were the most similar to ERR7738586 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738586,ERR7746321,ERR7738242,ERR7738559,ERR7738659,ERR7738660,ERR7746365,ERR7745896,ERR7738561,ERR7747394. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567843. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: k__Bacteria (UID203)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738586 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7738586.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: nucleic acid extraction

text: uncultured Fusicatenibacter sp.

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Fusicatenibacter;s__Fusicatenibacter sp900772675

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745937) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561471) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_N_23_1513.156

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics.

characteristic: assembly quality

text: metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz

characteristic: assembly software

text: Reads from ERR7745937 were assembled into contigs (ERZ4561471). The following read sets from this study were the most similar to ERR7745937 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745937,ERR7738176,ERR7738570,ERR7746785,ERR7745397,ERR7738251,ERR7738561,ERR7738631,ERR7738242,ERR7745423. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561471. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7745937 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7745937.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

characteristic: number of standard tRNAs extracted

text: uncultured Fusicatenibacter sp.

characteristic: organism

text: No

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

text: GTDB-TK v1.7.0, r202 database, default parameters

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7738199) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4560895) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_J_9_1548.5

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7738199 were assembled into contigs (ERZ4560895). The following read sets from this study were the most similar to ERR7738199 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738199,ERR7745874,ERR7745967,ERR7738154,ERR7746144,ERR7738196,ERR7738200,ERR7738178,ERR7738270,ERR7738225. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4560895. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

text: CheckM marker lineage: o__Clostridiales (UID1226)

characteristic: completeness score

characteristic: completeness software

text: reads

text: All reads in ERR7738199 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

text: This sample represents an uncultured Fusicatenibacter sp. MAG assembled and binned from ERR7738199.

text: terrestrial biome

text: human-associated habitat

text: feces

text: Tanzania

characteristic: inter-study species representative

characteristic: intra-study species representative

text: metagenome-assembled genome

characteristic: investigation type

text: human feces

characteristic: isolation source

text: human gut metagenome

characteristic: metagenomic source

characteristic: nucleic acid extraction

text: uncultured Fusicatenibacter sp.

text: Metagenome sequencing of Hadza gut microbiome

characteristic: project name

text: No

characteristic: reassembly post binning

characteristic: reference for biomaterial

characteristic: sample collection device or method

characteristic: sample derived from

text: uncultured Fusicatenibacter sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: tRNA extraction software

characteristic: taxonomic classification

text: multi-marker approach


characteristic: 16S rRNAs recovered

text: FALSE

characteristic: 16S recovered

text: barrnap;0.9;--kingdom bac

characteristic: 16S recovery software

characteristic: 23S rRNAs recovered

characteristic: 5S rRNAs recovered

characteristic: CheckM strain heterogeneity

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__UBA11957;s__

characteristic: GTDB r202 classification

text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: MAG coverage depth

text: Reads (ERR7745335) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561292) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15.

characteristic: MAG coverage software

characteristic: SRA accession

text: REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_D_16_1621.307

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: Reads from ERR7745335 were assembled into contigs (ERZ4561292). The following read sets from this study were the most similar to ERR7745335 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745335,ERR7738567,ERR7747394,ERR7746688,ERR7745823,ERR7738959,ERR7747255,ERR7745947,ERR7738569,ERR7745859. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561292. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin.

characteristic: binning parameters

text: MetaBAT2 v2.15; MAGpurify v2.1.0

characteristic: binning software

characteristic: collection date

characteristic: completeness approach

characteristic: contamination score

text: reads

characteristic: contamination screening input

text: All reads in ERR7745335 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2).

characteristic: contamination screening parameters

text: This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7745335.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: human-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: human feces

text: human gut metagenome

text: https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103

characteristic: number of standard tRNAs extracted

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: No

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach