Legionella pneumophila (DA38605)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38605)

text: Folkhlsomyndigheten

characteristic: collected by

characteristic: collection date

text: Legionella pneumophila environmental isolate DA38605 by Folkhlsomyndigheten

text: Sweden

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38607)

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Legionella pneumophila (DA38607)

tag: Namespace:UPPSALA UNIVERSITY

characteristic: Submitter Id

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38607 by Folkhlsomyndigheten

characteristic: description

text: Sweden

text: not provided

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: environmental

characteristic: isolation source

text: Legionella pneumophila

characteristic: organism

text: Legionella pneumophila

characteristic: title


Legionella pneumophila (DA38609)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38609)

text: Folkhlsomyndigheten

characteristic: collected by

characteristic: collection date

text: Legionella pneumophila environmental isolate DA38609 by Folkhlsomyndigheten

text: Sweden

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38611)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38611)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38611 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38613)

text: UPPSALA UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Legionella pneumophila (DA38613)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38613 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38615)

text: UPPSALA UNIVERSITY

text: public

characteristic: SRA accession

text: Legionella pneumophila (DA38615)

characteristic: Submitter Id

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38615 by Folkhlsomyndigheten

characteristic: description

text: Sweden

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38617)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38617)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38617 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

characteristic: isolate

text: environmental

characteristic: isolation source

text: Legionella pneumophila

characteristic: organism

text: Legionella pneumophila

characteristic: title


Legionella pneumophila (DA38619)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38619)

text: Folkhlsomyndigheten

characteristic: collected by

characteristic: collection date

text: Legionella pneumophila environmental isolate DA38619 by Folkhlsomyndigheten

text: Sweden

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38621)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38621)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38621 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38623)

text: UPPSALA UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Legionella pneumophila (DA38623)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38623 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38625)

text: UPPSALA UNIVERSITY

text: public

characteristic: SRA accession

text: Legionella pneumophila (DA38625)

characteristic: Submitter Id

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38625 by Folkhlsomyndigheten

characteristic: description

text: Sweden

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38627)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38627)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38627 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

characteristic: isolate

text: environmental

characteristic: isolation source

text: Legionella pneumophila

characteristic: organism

text: Legionella pneumophila

characteristic: title


Legionella pneumophila (DA38629)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38629)

text: Folkhlsomyndigheten

characteristic: collected by

characteristic: collection date

text: Legionella pneumophila environmental isolate DA38629 by Folkhlsomyndigheten

text: Sweden

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38631)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA38631)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38631 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38633)

text: UPPSALA UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Legionella pneumophila (DA38633)

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38633 by Folkhlsomyndigheten

text: Sweden

text: not provided

text: Homo sapiens

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA38637)

text: UPPSALA UNIVERSITY

text: public

characteristic: SRA accession

text: Legionella pneumophila (DA38637)

characteristic: Submitter Id

text: Folkhlsomyndigheten

text: Legionella pneumophila environmental isolate DA38637 by Folkhlsomyndigheten

characteristic: description

text: Sweden

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: environmental

text: Legionella pneumophila

text: Legionella pneumophila


Legionella pneumophila (DA39724)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA39724)

text: Legionella pneumophila environmental (cooling tower) isolate DA39724 by Legionella National Reference Laboratory in Spain

text: Spain

text: not provided

text: Homo sapiens

characteristic: isolate

text: environmental (cooling tower)

characteristic: isolation source

text: Legionella pneumophila

characteristic: organism

text: Legionella pneumophila

characteristic: title


Legionella pneumophila (DA39743)

text: UPPSALA UNIVERSITY

text: public

text: Legionella pneumophila (DA39743)

characteristic: collected by

characteristic: collection date

text: Legionella pneumophila environmental (cooling tower) isolate DA39743 by Legionella National Reference Laboratory in Spain

text: Spain

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: environmental (cooling tower)

text: Legionella pneumophila

text: Legionella pneumophila


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Zoology

text: public

tag: Namespace:Department of Zoology

text: western gorilla

characteristic: common name

text: Gorilla gorilla

text: Gorilla gorilla


text: Department of Zoology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: western gorilla

text: strongylids

text: Gorilla gorilla

text: Gorilla gorilla


E-MTAB-11226:Sample 1

text: Wellcome Sanger Institute

text: public

text: E-MTAB-11226:Sample 1

tag: Namespace:Wellcome Sanger Institute

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: peripheral blood mononuclear cell

characteristic: cell type

text: human

text: Protocols: PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quickly at 37 °C in a water bath. Two pools made for technical duplicates with ~500,000 cells for each donor per pool (50/50). Nuclei isolation, transposition, ATAC-seq and Gene Expression (GEX) sequencing libraries construction performed according to the manufacturer’s Demonstrated protocol (CG000365 Rev A; 10X Genomics) and Next GEM Single Cell Multiome ATAC and Gene Expression user guide (CG000338 Rev A; 10X Genomics). Nuclei isolation, transposition, ATAC-seq and Gene Expression (GEX) sequencing libraries construction performed according to the manufacturer’s Demonstrated protocol (CG000365 Rev A; 10X Genomics) and Next GEM Single Cell Multiome ATAC and Gene Expression user guide (CG000338 Rev A; 10X Genomics).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-11226:Sample 2

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute

text: public

text: E-MTAB-11226:Sample 2

text: ArrayExpress

text: peripheral blood mononuclear cell

text: human

text: adult

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 2

characteristic: title


E-MTAB-10643:Expt 1 14d post-amputation

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10643:Expt 1 14d post-amputation

tag: Namespace:IGF, CNRS, INSERM, Univ. Montpellier, LabEx ICST, F-34094 Montpellier, France.

unit: month

characteristic: age

text: ArrayExpress

text: interstitial cell

text: zebrafish

text: Protocols: Zebrafish were euthanized in tricaine and hearts were extracted in PBS Hearts were pooled and pre-digested for 1h in 1/10 TrypLE/HBSS (No CaMg) on ice. Hearts were then digested in Collagenase IV (5mg/ml) + Collagenase II (5mg/ml) in HBSS with 12.5uM CaCl2 at 32 deg on a shaker (800rpm) for 45 min. Following this, tissue was gently dissociated and passed through a cell strainer (40um), spun down (5min 300g) and resuspended in HBSS 2% fetal calf serum. The heart was exposed and approximately 20% of the ventricle removed Amplified cDNA was used for 3’ RNA-seq library generation, RNA-seq libraries were prepared following the manufacturer’s user guide (10x Genomics).

text: adult

text: wild type genotype

characteristic: genotype

text: 20 percent removal of the cardiac ventricle apex

characteristic: injury

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: male

characteristic: sex

text: AB

characteristic: strain

text: Expt 1 14d post-amputation


E-MTAB-10643:Expt 1 3d post-amputation

text: IGF, CNRS, INSERM, Univ. Montpellier, LabEx ICST, F-34094 Montpellier, France.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10643:Expt 1 3d post-amputation

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: interstitial cell

text: zebrafish

characteristic: common name

text: adult

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: male

text: AB

text: Expt 1 3d post-amputation


E-MTAB-10643:Expt 1 7d post-amputation

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10643:Expt 1 7d post-amputation

text: ArrayExpress

text: interstitial cell

characteristic: cell type

text: zebrafish

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: heart

characteristic: organism part

text: Danio rerio

text: male

text: AB

text: Expt 1 7d post-amputation

characteristic: title


E-MTAB-10643:Expt 1 Uninjured

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10643:Expt 1 Uninjured

characteristic: age

text: ArrayExpress

text: interstitial cell

text: zebrafish

text: adult

text: wild type genotype

characteristic: genotype

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: male

characteristic: sex

text: AB

text: Expt 1 Uninjured


name: E-MTAB-10643:Expt 3 3d post-amputation + MMP inhibitor

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: interstitial cell

text: zebrafish

text: Protocols: Zebrafish were euthanized in tricaine and hearts were extracted in PBS Hearts were pooled and pre-digested for 1h in 1/10 TrypLE/HBSS (No CaMg) on ice. Hearts were then digested in Collagenase IV (5mg/ml) + Collagenase II (5mg/ml) in HBSS with 12.5uM CaCl2 at 32 deg on a shaker (800rpm) for 45 min. Following this, tissue was gently dissociated and passed through a cell strainer (40um), spun down (5min 300g) and resuspended in HBSS 2% fetal calf serum. The heart was exposed and approximately 20% of the ventricle removed Zebrafish were treated with MMP inhibitor (NSC405020) for three days following surgery. Amplified cDNA was used for 3’ RNA-seq library generation, RNA-seq libraries were prepared following the manufacturer’s user guide (10x Genomics).

text: adult

text: wild type genotype

characteristic: injury

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: male

text: AB

characteristic: strain

text: matrix metalloproteinase inhibitor

characteristic: treatment


E-MTAB-10643:Expt 2 3d post-amputation

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10643:Expt 2 3d post-amputation

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: interstitial cell

characteristic: cell type

text: zebrafish

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: heart

text: Danio rerio

text: male

text: AB

text: Expt 2 3d post-amputation


E-MTAB-10643:Expt 2 Sham

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10643:Expt 2 Sham

characteristic: age

text: ArrayExpress

text: interstitial cell

text: zebrafish

text: adult

text: wild type genotype

characteristic: genotype

text: sham surgery

text: Danio rerio

text: Danio rerio

text: heart

characteristic: organism part

text: Danio rerio

text: male

characteristic: sex

text: AB

text: Expt 2 Sham

characteristic: title


E-MTAB-10643:Expt 2 Uninjured

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10643:Expt 2 Uninjured

text: ArrayExpress

text: interstitial cell

text: zebrafish

text: adult

text: wild type genotype

text: Danio rerio

text: Danio rerio

text: heart

text: Danio rerio

text: male

text: AB

characteristic: strain

text: Expt 2 Uninjured


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB)

characteristic: Submitter Id

text: not available

unit: week

text: ArrayExpress

characteristic: broker name

text: endothelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: human eye - hCEC: Human eyes were provided by the Biobank of UZ Leuven Hospital. Only leftover material after use for cornea transplantation was used. The choroidal membranes were dissected and processed for scRNA-seq upon dissociating into single cell suspension in a digestion buffer (0.3% collagenase I, DNase I (7.5 M) and dispase (2.5 U ml-1) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA USA) for 40 min in 37 oC with manual pipetting every 10 min. Single cell suspension was enriched for ECs by MACS depletion of CD45 positive cells and subsequent enrichment of CD31 positive cells (Miltenyi Biotec). murine samples - CECs and CNV: Choroidal neovascularization (CNV) was induced by laser burns as previously described with adaptations (Schoors et al., 2014) using a Purepoint Laser (Alcon, Fort Worth, USA). Ten impacts rupturing the Bruch’s membrane were made around the optical nerve using laser diameter 100 µm, power 0.320 W and exposure time 0.05 seconds in both eyes of 6 mice per group. At day 7, at the height of the angiogenic response (Lambert et al., 2013), mice were sacrificed by cervical dislocation and the eyes were enucleated. The choroid-sclera complex was dissected by peeling off the vitreous body and retina, pooled per group, and processed for scRNA-seq upon dissociating into single cell suspension in a digestion buffer (0.3% collagenase I, DNase I (7.5 M) and dispase (2.5 U ml-1) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin for 40 min in 37 oC with manual pipetting every 10 min. The reaction was stopped with 10 ml of isolation buffer and the cell suspension was filtered through a 100 µm cell strainer. Single cell suspensions were enriched for ECs by MACS depletion of CD45 positive cells and subsequent enrichment of CD31 positive cells (Miltenyi Biotec). This procedure was repeated for 3 independent replicate experiments, and 3 additional experiments were performed to obtain reference samples of ECs isolated from healthy choroid of control mice without laser injury (referred to as healthy CECs; n=2 eyes/mouse and n=6 mice per experimental group). Nucleic acid extraction was done in single cells using the Chromium Single Cell 3’ Library, Gel Bead & Multiplex Kit and Chip Kit (v2, 10x Genomics) according to manufacturer’s instructions. The single cell suspensions of freshly isolated choroidal ECs were converted to barcoded scRNA-seq libraries using the Chromium Single Cell 3’ Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics), aiming for 10,000 cells per library. Samples were processed using kits pertaining to v2 barcoding chemistry of 10x Genomics. Single samples were always processed in a single well of a PCR plate, allowing all cells from a sample to be treated with the same master mix and in the same reaction vessel. For each experiment, all samples (CNV-EC and CEC) were processed in parallel in the same thermal cycler.

characteristic: description

text: adult

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: eye

text: optic choroid

characteristic: sampling site

text: Homo sapiens

text: male

text: hCEC


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB)

text: public

text: 7 to 8

characteristic: age

text: ArrayExpress

text: endothelial cell

text: house mouse

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: eye

characteristic: organism part

text: optic choroid

text: Mus musculus

text: male


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 7 to 8

text: ArrayExpress

text: endothelial cell

text: house mouse

text: adult

text: choroidal neovascularization

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: eye

text: optic choroid

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford

characteristic: Submitter Id

unit: day

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Caudal parts from E9.5 (20-23 sp) Ncx1-/- and Ncx1+/+ littermate controls (4 embryos/group) were dissected and processed into single cell suspensions. Dead/damaged cells were removed by magnetic separation. As per 10x Genomics manufacture's protocol As per 10x Genomics manufacture's protocol

characteristic: description

text: embryonic day 9.5

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: para-aortic splanchnopleura

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Molecular Hematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford

text: public

characteristic: age

text: ArrayExpress

text: house mouse

text: embryonic day 9.5

characteristic: developmental stage

text: Ncx1 knockout

text: Mus musculus

text: Mus musculus

text: para-aortic splanchnopleura

text: Mus musculus


text: Royal Botanic Garden Edinburgh

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: NOT PROVIDED

characteristic: barcoding center

text: COPO

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

characteristic: collected by

text: UNIVERSITY OF EDINBURGH

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Stirling | Strathyre forest

characteristic: geographic location (region and locality)

text: Grassland, roadside, riverside

characteristic: habitat

text: Chris Metherell

characteristic: identified by

text: PRIVATE

text: sporophyte

characteristic: lifestage

text: Euphrasia arctica

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Euphrasia arctica

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: c736e5af-23a9-44ea-a2e9-40a47ddad32f-DTOL-specimen

characteristic: tolid

type: derived from


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Garden Edinburgh

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NOT PROVIDED

characteristic: barcoding center

text: COPO

characteristic: broker name

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

text: UNIVERSITY OF EDINBURGH

text: United Kingdom

text: Highland | East Knockbain Wood

text: NOT COLLECTED

text: Chris Metherell

text: PRIVATE

text: sporophyte

text: Euphrasia arctica

text: WHOLE ORGANISM

text: DTOL

text: Euphrasia arctica

text: NOT PROVIDED

text: 842a9cae-8d31-419e-b7a0-5db23c0c2b0f-DTOL-specimen

characteristic: title


text: Royal Botanic Garden Edinburgh

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: NOT PROVIDED

text: COPO

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

characteristic: collected by

text: UNIVERSITY OF EDINBURGH

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Highland | Lairg

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: Chris Metherell

characteristic: identified by

text: PRIVATE

text: sporophyte

characteristic: lifestage

text: Euphrasia arctica

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Euphrasia arctica

characteristic: specimen id

text: NOT PROVIDED

text: ca969745-bed6-4b8a-b156-6421a7cbd72f-DTOL-specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Garden Edinburgh

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NOT PROVIDED

characteristic: barcoding center

text: COPO

characteristic: broker name

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

text: UNIVERSITY OF EDINBURGH

text: United Kingdom

text: Highland | Lairg

text: NOT COLLECTED

text: Chris Metherell

text: PRIVATE

text: sporophyte

text: Euphrasia arctica

text: WHOLE ORGANISM

text: DTOL

text: Euphrasia arctica

text: NOT PROVIDED

characteristic: specimen voucher

text: 7bd07e94-989c-4735-9cc0-c5238376b81d-DTOL-specimen

characteristic: tolid


text: Royal Botanic Garden Edinburgh

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: NOT PROVIDED

text: COPO

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

characteristic: collected by

text: UNIVERSITY OF EDINBURGH

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Highland | Garve | Loch Glasscarnoch

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: Chris Metherell

characteristic: identified by

text: PRIVATE

text: sporophyte

characteristic: lifestage

text: Euphrasia micrantha

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Euphrasia micrantha

text: NOT PROVIDED

text: 66fbd383-4d8a-438b-9518-9eb5d98b5ddc-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Garden Edinburgh

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NOT PROVIDED

characteristic: barcoding center

text: COPO

characteristic: broker name

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

text: UNIVERSITY OF EDINBURGH

text: United Kingdom

text: Highland | Lochrosque | Achnasheen

text: NOT COLLECTED

text: Chris Metherell

text: PRIVATE

text: sporophyte

text: Euphrasia micrantha

text: WHOLE ORGANISM

text: DTOL

text: Euphrasia micrantha

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: 5e9c8bd7-35a1-41fe-adb0-5170a94a7552-DTOL-specimen


text: Royal Botanic Garden Edinburgh

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: NOT PROVIDED

text: COPO

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

characteristic: collected by

text: UNIVERSITY OF EDINBURGH

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Highland | Lubmore | Achnasheen

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: Chris Metherell

characteristic: identified by

text: PRIVATE

text: sporophyte

characteristic: lifestage

text: Euphrasia arctica

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: DTOL

characteristic: project name

text: Euphrasia arctica

text: NOT PROVIDED

text: a529e2a2-a5aa-461b-9828-8237c9f61db3-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Royal Botanic Garden Edinburgh

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: NOT PROVIDED

characteristic: barcoding center

text: COPO

characteristic: broker name

text: YANQIAN DING | MENG LU | ROWAN SCHLEY

text: UNIVERSITY OF EDINBURGH

text: United Kingdom

text: Highland | Lubmore | Achnasheen

text: NOT COLLECTED

text: Chris Metherell

text: PRIVATE

text: sporophyte

text: Euphrasia micrantha

text: WHOLE ORGANISM

text: DTOL

text: Euphrasia micrantha

characteristic: specimen id

text: NOT PROVIDED

text: de6c419f-f13a-4791-85d0-a05a84b00fde-DTOL-specimen

characteristic: title


text: Helmholtz Munich

text: public

tag: Namespace:Helmholtz Munich

text: 74 to 80

characteristic: age

text: ArrayExpress

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: BMMNC were stained with conjugated monoclonal antibodies for HSPC markers as described above. 10,000 Lin-kit+ cells from young or aged WT/Sema4AKO mice were sorted in Ca2+/Mg2+-free phosphate-buffered saline (D-PBS) supplemented with 2% bovine serum albumin (New England Biolabs). Following one round of centrifugation, the cells were processed on 10x Genomics platform according to the manufacturer’s protocol. Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer’s protocol. Libraries from 10,000 Kit+ cells each from WT (n=1) and Sema4A KO (n=2) young and WT (n=2) old mice were prepared using the Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer’s protocol.

characteristic: description

text: wild type genotype

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus


text: Helmholtz Munich

text: public

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i701_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i701_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i701_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i701_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i701_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i701_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i701_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i701_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i701_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i701_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i701_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i702_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i702_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i702_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i702_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i702_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i702_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i702_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i702_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i702_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i702_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i702_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i703_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i703_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i703_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i703_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i703_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i703_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i703_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i703_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i703_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i703_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i703_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i704_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i704_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i704_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i704_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i704_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i704_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i704_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i704_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i704_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i704_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i704_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i705_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i705_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i705_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i705_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i705_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i705_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i705_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i705_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i705_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i705_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i705_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i706_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i706_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i706_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i706_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i706_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i706_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i706_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i706_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i706_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i706_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i706_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i707_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i707_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i707_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i707_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title