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tag: Namespace:Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium  VIB Center for Plant Systems Biology, VIB, Gent, Belgium

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text: Protocols: Collection of Medicago truncatula hairy root lines grown in vitro at the VIB-UGent center for Plant Systems Biology in Gent, Belgium. Sterilization of M. truncatula seeds (ecotype Jemalong J5), transformation of seedlings by Agrobacterium rhizogenes (strain LBA 9402/12), and the subsequent generation of hairy roots were carried out as described previously (Pollier et al., 2011, J. Nat. Prod. 74, 1462-1476.). Hairy roots were cultivated for 21 days in liquid medium to provide proper amounts for RNA extraction. For elicitation of triterpene saponin pathway gene expression, 100 µM MeJA was added to the medium for 4 h (or an equivalent of the solvent EtOH for mock treatment) prior to sampling Frozen hairy roots were ground and the material was used to prepare total RNA using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, poly(A)-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated, and adapters were ligated. After quality control using the 2100 Bioanalyzer, clusters were generated through amplification using the TruSeq kit (Illumina) followed by sequencing on Illumina NextSeq 500 with the TruSeq SBS Kit v3-HS (Illumina). Sequencing was performed in single-end mode with a read length of 75nt.

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HG02572 GWD

text: Open Genomes

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text: human

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text: Homo sapiens

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text: Shenzhen Puensum Genetech Institute

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text: China

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text: ena-SAMPLE-TAB-28-04-2022-10:23:35:296-12536

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text: Diatom eDNA metabarcoding in the peryphyton of a karstic river using the rbcl gene marker

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text: ena-SAMPLE-TAB-28-04-2022-10:23:35:299-12566

text: Diatom eDNA metabarcoding in the peryphyton of a karstic river using the rbcl gene marker

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tag: Namespace:Max Planck Institute for Evolutionary Anthropology

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text: human

characteristic: common name

text: Germany

characteristic: geographic location (country and/or sea)

text: Saxony-Anhalt, Germany

characteristic: geographic location (region and locality)

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Max Planck Institute for Evolutionary Anthropology

text: public

text: human

text: Germany

text: Saxony-Anhalt, Germany

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


E-MTAB-11103:Sample 13

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 13

tag: Namespace:Instituto de Investigacion Sanitaria La Fe

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: Plasma samples were obtained from all participants. They were stored at -80 ºC 700 µL QIAzol lysis reagent were added to 200 µL plasma, vortexed and incubated at 20-25 ºC for 5 min. After that, 140 µL of chloroform were added and incubated at room temperature for 3 min. Then, it was centrifuged (15min, 12000g, 4 ºC), and the aqueous phase transferred to a new tube containing 525 µL ethanol. The mixture was transferred into an RNeasy MinElute spin column, centrifuged (10000g, 30s, RT). Column was washed with 700 µL buffer RWT and twice with 500 µL buffer RPE. Membrane was dried by centrifugation (10000g, 90s) and elution was carried out in 15 µL RNase-free water centrifuging (1 min, 13000g) the smallRNA molecules are first ligated to the 3'- 4N adenylated adapters, taking advantage of the phosphate group at their terminal end, which allows these molecules to be exclusively selected. Second the 5'-4N adapters are ligated. After ligation of the adapters, the reverse transcription of the molecule to cDNA is carried out. The generated cDNA fragments are amplified and indexed by PCR using different barcode primers for each sample. Finally a purification with size selection is carried out. The quality control and concentration of the libraries are verified with the Agilent Technologies 2100 bioanalyzer using highly sensitive DNA chips. Subsequently, an equimolecular pool of each library is prepared for sequencing on the NGS NextSeq 550 platform (Illumina, San Diego, CA, USA) by single read sequencing of 50 cycles (1x50 bp).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: control

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 13


E-MTAB-11103:Sample 16

characteristic: External Id

text: Instituto de Investigacion Sanitaria La Fe

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 16

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 16


E-MTAB-11103:Sample 22

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 22

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 22


E-MTAB-11103:Sample 23

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 23

characteristic: age

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 23

characteristic: title


E-MTAB-11103:Sample 28

text: public

text: E-MTAB-11103:Sample 28

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 28


E-MTAB-11103:Sample 31

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 31

text: ArrayExpress

text: human

text: adult

text: normal

text: control

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 31


E-MTAB-11103:Sample 33

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 33

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 33


E-MTAB-11103:Sample 34

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 34

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 34


E-MTAB-11103:Sample 36

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 36

characteristic: age

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 36

characteristic: title


E-MTAB-11103:Sample 37

text: public

text: E-MTAB-11103:Sample 37

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 37


E-MTAB-11103:Sample 38

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 38

text: ArrayExpress

text: human

text: adult

text: normal

text: control

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 38


E-MTAB-11103:Sample 39

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 39

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 39


E-MTAB-11103:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 4

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 4


E-MTAB-11103:Sample 40

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 40

characteristic: age

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 40

characteristic: title


E-MTAB-11103:Sample 41

text: public

text: E-MTAB-11103:Sample 41

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 41


E-MTAB-11103:Sample 42

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 42

text: ArrayExpress

text: human

text: adult

text: normal

text: control

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 42


E-MTAB-11103:Sample 43

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 43

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 43


E-MTAB-11103:Sample 44

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 44

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: normal

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 44


E-MTAB-11103:Sample 7

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 7

characteristic: age

text: ArrayExpress

text: human

text: adult

text: normal

text: control

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 7

characteristic: title


E-MTAB-11103:Sample 8

text: public

text: E-MTAB-11103:Sample 8

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: control

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 8


E-MTAB-11103:Sample 1

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 1

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-11103:Sample 11

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 11

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 11


E-MTAB-11103:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 12

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 12


E-MTAB-11103:Sample 14

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 14

characteristic: age

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 14

characteristic: title


E-MTAB-11103:Sample 15

text: public

text: E-MTAB-11103:Sample 15

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: Alzheimer disease

characteristic: disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 15


E-MTAB-11103:Sample 17

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 17

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 17


E-MTAB-11103:Sample 18

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 18


E-MTAB-11103:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 19

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 19


E-MTAB-11103:Sample 20

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 20

characteristic: age

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 20

characteristic: title


E-MTAB-11103:Sample 21

text: public

text: E-MTAB-11103:Sample 21

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: Alzheimer disease

characteristic: disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 21


E-MTAB-11103:Sample 24

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 24

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 24


E-MTAB-11103:Sample 25

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 25


E-MTAB-11103:Sample 26

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 26

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 26


E-MTAB-11103:Sample 27

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 27

characteristic: age

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 27

characteristic: title


E-MTAB-11103:Sample 3

text: public

text: E-MTAB-11103:Sample 3

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: Alzheimer disease

characteristic: disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 3


E-MTAB-11103:Sample 45

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 45

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 45


E-MTAB-11103:Sample 46

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 46

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 46


E-MTAB-11103:Sample 47

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 47

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 47


E-MTAB-11103:Sample 48

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 48

characteristic: age

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: mild cognitive impairment

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 48

characteristic: title


E-MTAB-11103:Sample 5

text: public

text: E-MTAB-11103:Sample 5

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: Alzheimer disease

characteristic: disease

text: mild cognitive impairment

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 5


E-MTAB-11103:Sample 10

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 10

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 10


E-MTAB-11103:Sample 2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 2


E-MTAB-11103:Sample 29

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 29

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 29


E-MTAB-11103:Sample 30

text: public

characteristic: INSDC status

text: E-MTAB-11103:Sample 30

characteristic: age

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 30

characteristic: title


E-MTAB-11103:Sample 32

text: public

text: E-MTAB-11103:Sample 32

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: Alzheimer disease

characteristic: disease

text: preclinical Alzheimer disease

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 32


E-MTAB-11103:Sample 35

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11103:Sample 35

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: blood plasma

characteristic: organism part

text: Homo sapiens

text: Sample 35


E-MTAB-11103:Sample 6

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11103:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 6


E-MTAB-11103:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11103:Sample 9

text: ArrayExpress

text: human

characteristic: common name

text: adult

text: Alzheimer disease

text: preclinical Alzheimer disease

text: Homo sapiens

text: Homo sapiens

text: blood plasma

text: Homo sapiens

text: Sample 9


text: IP

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: ct value

text: Protocols: Blood, serum or urine sample DNeasy Blood and Tissue DNA extraction kit Sureselect Xt HS Target Enrichment System for Illumina

text: wild type genotype

characteristic: genotype

text: Leptospira kirschneri

characteristic: organism

text: Leptospira kirschneri

text: whole organism

text: blood serum

characteristic: sampling site

text: Leptospira kirschneri

characteristic: strain

characteristic: title


characteristic: External Id

text: IP

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

characteristic: description

text: wild type genotype

text: Leptospira kirschneri

text: Leptospira kirschneri

text: whole organism

text: blood

text: Leptospira kirschneri


text: IP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

text: wild type genotype

text: Leptospira interrogans

text: Leptospira interrogans

text: whole organism

characteristic: organism part

text: blood

text: Leptospira interrogans


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: IP

text: public

characteristic: Submitter Id

text: ArrayExpress

text: wild type genotype

text: Leptospira interrogans

text: Leptospira interrogans

text: whole organism

text: urine

text: Leptospira interrogans


text: IP

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: ct value

text: wild type genotype

characteristic: genotype

text: Leptospira kirschneri

text: Leptospira kirschneri

text: whole organism

text: blood

text: Leptospira kirschneri


E-MTAB-11670:HepaG2 Cd1

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 Cd1

tag: Namespace:North Carolina Central University

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

characteristic: common name

text: Protocols: HepaRG and HepaG2 cells treated with physiological concentrations of Cadmiun or PFOAs for 2 months in triplicate and samples were collected. Total RNA was isolated using TRIZOl reagents (Sigma). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3’ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).

text: liver cancer

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: HepaG2 Cd1


E-MTAB-11670:HepaG2 Cd2

characteristic: External Id

text: North Carolina Central University

characteristic: INSDC center name

text: public

text: E-MTAB-11670:HepaG2 Cd2

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

characteristic: description

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 Cd2

characteristic: title


E-MTAB-11670:HepaG2 Cd3

characteristic: ENA-CHECKLIST

text: North Carolina Central University

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11670:HepaG2 Cd3

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 Cd3


E-MTAB-11670:HepaG2 control 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: North Carolina Central University

text: public

characteristic: INSDC status

text: E-MTAB-11670:HepaG2 control 1

characteristic: Submitter Id

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control 1


E-MTAB-11670:HepaG2 control 2

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 control 2

text: ArrayExpress

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control 2


E-MTAB-11670:HepaG2 control 3

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 control 3

text: ArrayExpress

text: hepatocyte

text: human

characteristic: common name

text: liver cancer

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control 3


E-MTAB-11670:HepaG2 control PFOAs 1

characteristic: External Id

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 control PFOAs 1

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control PFOAs 1


E-MTAB-11670:HepaG2 control PFOAs 2

text: North Carolina Central University

characteristic: INSDC center name

text: public

text: E-MTAB-11670:HepaG2 control PFOAs 2

text: ArrayExpress

characteristic: broker name

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control PFOAs 2


E-MTAB-11670:HepaG2 control PFOAs 3

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 control PFOAs 3

text: ArrayExpress

text: hepatocyte

text: human

characteristic: description

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 control PFOAs 3

characteristic: title


E-MTAB-11670:HepaG2 PFOAs 1

characteristic: ENA-CHECKLIST

text: North Carolina Central University

characteristic: INSDC first public

text: public

text: E-MTAB-11670:HepaG2 PFOAs 1

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 PFOAs 1


E-MTAB-11670:HepaG2 PFOAs 2

text: North Carolina Central University

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11670:HepaG2 PFOAs 2

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 PFOAs 2


E-MTAB-11670:HepaG2 PFOAs 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaG2 PFOAs 3

text: ArrayExpress

text: hepatocyte

text: human

text: liver cancer

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaG2 PFOAs 3


E-MTAB-11670:HepaRG Cd1

text: North Carolina Central University

text: public

characteristic: INSDC status

text: E-MTAB-11670:HepaRG Cd1

characteristic: Submitter Id

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG Cd1


E-MTAB-11670:HepaRG Cd2

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG Cd2

text: ArrayExpress

text: HepaRG

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG Cd2


E-MTAB-11670:HepaRG Cd3

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG Cd3

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

characteristic: common name

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG Cd3


E-MTAB-11670:HepaRG control 1

characteristic: External Id

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG control 1

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: HepaRG control 1


E-MTAB-11670:HepaRG control 2

text: North Carolina Central University

characteristic: INSDC center name

text: public

text: E-MTAB-11670:HepaRG control 2

text: ArrayExpress

characteristic: broker name

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG control 2


E-MTAB-11670:HepaRG control 3

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG control 3

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

characteristic: description

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG control 3

characteristic: title


E-MTAB-11670:HepaRG control PFOAs 1

characteristic: ENA-CHECKLIST

text: North Carolina Central University

characteristic: INSDC first public

text: public

text: E-MTAB-11670:HepaRG control PFOAs 1

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG control PFOAs 1


E-MTAB-11670:HepaRG control PFOAs 2

text: North Carolina Central University

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-11670:HepaRG control PFOAs 2

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG control PFOAs 2


E-MTAB-11670:HepaRG control PFOAs 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG control PFOAs 3

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG control PFOAs 3


E-MTAB-11670:HepaRG PFOAs 1

text: North Carolina Central University

text: public

characteristic: INSDC status

text: E-MTAB-11670:HepaRG PFOAs 1

characteristic: Submitter Id

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG PFOAs 1


E-MTAB-11670:HepaRG PFOAs 2

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG PFOAs 2

text: ArrayExpress

text: HepaRG

characteristic: cell line

text: hepatocyte

characteristic: cell type

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG PFOAs 2


E-MTAB-11670:HepaRG PFOAs 3

text: North Carolina Central University

text: public

text: E-MTAB-11670:HepaRG PFOAs 3

text: ArrayExpress

text: HepaRG

text: hepatocyte

text: human

characteristic: common name

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: HepaRG PFOAs 3


E-MTAB-10570_2:Sample 1

characteristic: External Id

text: public

text: E-MTAB-10570_2:Sample 1

tag: Namespace:Wellcome Sanger Institute, Cambridge, UK

unit: week

characteristic: age

text: ArrayExpress

text: human

text: Protocols: All tissue samples used for this study were obtained with written informed consent from all participants in accordance with the guidelines in The Declaration of Helsinki 2000. Human embryo and fetal samples were obtained from the MRC and Wellcome-funded Human Developmental Biology Resource (HDBR43, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Newcastle and North Tyneside NHS Health Authority Joint Ethics Committee (08/H0906/21+5). The HDBR is regulated by the UK Human Tissue Authority (HTA; www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice. All tissues for sequencing and spatial work were collected in saline (HypoThermosol biopreservation media) and stored 4°C until processing. Tissue dissociation was conducted within 24 hours of tissue retrieval with the exception of tissues that were cryopreserved and stored in -80°C. We used the previous protocol optimised for gonadal dissociation 5 and available at protocols.io In short, tissues were cut into <1 mm segments before being transferred to a 15ml tube. Tissues were digested with Trypsin/EDTA 0.25% between 5-15 minutes at 37°C with intermittent shaking. Digested tissue was passed through a 100 µm filter, and cells collected by centrifugation (500 x g for 5 minutes at 4°C). Cells were washed with flow buffer (PBS containing 5 % (v/v) FBS and 2 mM EDTA) prior to cell counting. Single-nuclei suspension was produced from dissociated cells for doing scATAC-seq, following the manufacturers’ instructions, and from frozen tissue sections for doing the multiomic snRNA-seq/scATAC-seq. For the scATAC-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell ATAC v1.0 and v to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer’s protocol.

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

characteristic: sample

text: Homo sapiens

text: female

characteristic: sex

text: Sample 1


E-MTAB-10570_2:Sample 17

characteristic: ENA-CHECKLIST

text: Wellcome Sanger Institute, Cambridge, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-10570_2:Sample 17

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: late embryonic stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

text: Sample 17

characteristic: title


E-MTAB-10570_2:Sample 15

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

text: E-MTAB-10570_2:Sample 15

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

text: late embryonic stage

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

text: Sample 15


E-MTAB-10570_2:Sample 18

characteristic: External Id

text: public

text: E-MTAB-10570_2:Sample 18

characteristic: age

text: ArrayExpress

text: human

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 18


E-MTAB-10570_2:Sample 11

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10570_2:Sample 11

text: ArrayExpress

text: human

text: late embryonic stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

characteristic: sample

text: Homo sapiens

text: female

characteristic: sex

text: Sample 11


E-MTAB-10570_2:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: SRA accession

text: E-MTAB-10570_2:Sample 6

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: late embryonic stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

text: Sample 6

characteristic: title


E-MTAB-10570_2:Sample 20

characteristic: External Id

text: public

characteristic: INSDC status

text: E-MTAB-10570_2:Sample 20

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: late embryonic stage

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

text: Sample 20


E-MTAB-10570_2:Sample 8

characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

text: E-MTAB-10570_2:Sample 8

text: ArrayExpress

text: human

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 8


E-MTAB-10570_2:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10570_2:Sample 10

text: ArrayExpress

text: human

text: late embryonic stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

characteristic: sample

text: Homo sapiens

text: male

characteristic: sex

text: Sample 10


E-MTAB-10570_2:Sample 9

characteristic: External Id

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10570_2:Sample 9

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: late embryonic stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

text: Sample 9

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

text: metagenome from batch anaerobic bioreactors

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003965

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 515F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

unit: °C

characteristic: sample storage temperature

unit: mL

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

unit: ºC

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003966

text: control

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 516F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: control

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003967

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 517F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003968

text: control

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 518F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: control

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003969

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 519F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003970

text: control

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 520F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: control

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003971

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 521F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003972

text: control

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 522F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: control

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003973

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 523F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: control

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003974

text: control

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 524F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: control

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003975

characteristic: environment (material)

text: control

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 525F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: 14.9 g (NH4)2CO3 ammonium carbonate salt CHEBI:18367 CHEBI:41609 CHEBI:24871

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003976

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 526F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003977

characteristic: environment (material)

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 527F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003978

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 528F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003979

characteristic: environment (material)

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 529F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

text: metagenome from batch anaerobic bioreactors

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003980

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 530F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003981

characteristic: environment (material)

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 531F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003982

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 532F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003983

characteristic: environment (material)

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 533F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003984

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 534F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: 10.5 g zeolite

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003985

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 535F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003986

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 536F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: 10.5 g zeolite

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003987

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 537F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003988

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 538F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: 10.5 g zeolite

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: 16.6 g NH4CL ammonium chloride salt CHEBI:31206 CHEBI:24871

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003989

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 539F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003990

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 540F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003991

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 541F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003992

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 542F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003993

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 543F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRAE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: chemical administration

characteristic: collection date

characteristic: description

text: Batch anaerobic digester

characteristic: environment (biome)

text: anaerobic mesophilic biodigester

characteristic: environment (feature)

text: anaerobic digester sludge ENVO:00003994

characteristic: environment (material)

text: ammonia sources

text: France

text: Kapa HiFi DNA polymerase (10 cycles)?

text: DNeasy PowerSoil DNA Elution Kit

text: anaerobic digester metagenome

characteristic: organism

text: 544F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

text: STABILICS

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INRAE

text: public

text: metagenome from batch anaerobic bioreactors

text: Batch anaerobic digester

text: anaerobic mesophilic biodigester

text: anaerobic digester sludge ENVO:00003995

text: ammonia sources

characteristic: experimental factor

text: France

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Kapa HiFi DNA polymerase (10 cycles)?

characteristic: nucleic acid amplification

text: DNeasy PowerSoil DNA Elution Kit

characteristic: nucleic acid extraction

text: anaerobic digester metagenome

text: 545F:GTGYCAGCMGCCGCGGTA 928R:CCCCGYCAATTCMTTTRAGT

characteristic: pcr primers

text: 10.5 g zeolite

characteristic: perturbation

text: STABILICS

characteristic: project name

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: anaerobic digester metagenome

text: Ion Torrent PGM

characteristic: sequencing method

characteristic: target gene

characteristic: target subfragment

characteristic: temperature