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characteristic: ENA-CHECKLIST

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text: University of Antwerp

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text: BIOVEINS sample 228

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text: University of Antwerp

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characteristic: ENA-FIRST-PUBLIC

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text: University of Antwerp

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text: BIOVEINS sample 230

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characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Antwerp

text: public

characteristic: collection date

text: BIOVEINS sample 231

characteristic: description

text: city (ENVO:00000856)

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text: Anopheles gambiae

characteristic: ArrayExpress-Species

text: SC

text: public

text: African malaria mosquito

characteristic: common name

text: Anopheles gambiae

characteristic: organism

text: Anopheles gambiae

characteristic: title


E-MTAB-11583:Sample 17

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 17

tag: Namespace:Wageningen University and Research

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: Root systems were separated from leaves and removed from the medium. The root systems were immediately snap frozen in liquid nitrogen Plants were grown for 14 days in the 16/8 hours light/dark at 21 degrees celsius. After 14 days plants were transferred to the dark for the remaining time until sampling. Plants were grown in 6 wells plates with MS-20 medium with Gelrite. The Maxwell® 16 Tissue LEV plant RNA kit was used for mRNA isolation with a Maxwell® AS2000 instrument (Promega), following the manufacturer’s protocol. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are size-selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library.

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 17


E-MTAB-11583:Sample 18

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 18


E-MTAB-11583:Sample 19

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 19

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 19

characteristic: title


E-MTAB-11583:Sample 20

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 20

characteristic: age

text: ArrayExpress

text: thale cress

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 20


E-MTAB-11583:Sample 33

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 33

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 33


E-MTAB-11583:Sample 34

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 34

text: ArrayExpress

text: thale cress

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 34


E-MTAB-11583:Sample 35

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 35

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 35


E-MTAB-11583:Sample 36

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 36

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 36

characteristic: title


E-MTAB-11583:Sample 49

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 49

characteristic: age

text: ArrayExpress

text: thale cress

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 49


E-MTAB-11583:Sample 50

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 50

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 50


E-MTAB-11583:Sample 51

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characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 51

text: ArrayExpress

text: thale cress

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 51


E-MTAB-11583:Sample 52

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 52

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 52


E-MTAB-11583:Sample 1

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text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 1

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 1

characteristic: title


E-MTAB-11583:Sample 13

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 13

characteristic: age

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 13


E-MTAB-11583:Sample 14

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 14

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 14


E-MTAB-11583:Sample 15

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 15

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 15


E-MTAB-11583:Sample 16

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 16

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 16


E-MTAB-11583:Sample 2

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 2

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 2

characteristic: title


E-MTAB-11583:Sample 29

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 29

characteristic: age

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 29


E-MTAB-11583:Sample 3

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 3

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 3


E-MTAB-11583:Sample 30

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 30

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 30


E-MTAB-11583:Sample 31

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 31

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 31


E-MTAB-11583:Sample 32

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 32

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 32

characteristic: title


E-MTAB-11583:Sample 4

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 4

characteristic: age

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 4


E-MTAB-11583:Sample 45

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 45

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 45


E-MTAB-11583:Sample 46

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 46

text: ArrayExpress

text: thale cress

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 46


E-MTAB-11583:Sample 47

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 47

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: wild type genotype

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 47


E-MTAB-11583:Sample 48

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 48

text: ArrayExpress

text: thale cress

characteristic: common name

text: wild type genotype

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 48

characteristic: title


E-MTAB-11583:Sample 25

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 25

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 25


E-MTAB-11583:Sample 26

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 26

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 26


E-MTAB-11583:Sample 27

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 27

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 27


E-MTAB-11583:Sample 28

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 28

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 28


E-MTAB-11583:Sample 41

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 41

text: ArrayExpress

text: thale cress

characteristic: common name

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 41

characteristic: title


E-MTAB-11583:Sample 42

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 42

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 42


E-MTAB-11583:Sample 43

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 43

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 43


E-MTAB-11583:Sample 44

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 44

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 44


E-MTAB-11583:Sample 57

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 57

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 57


E-MTAB-11583:Sample 58

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 58

text: ArrayExpress

text: thale cress

characteristic: common name

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 58

characteristic: title


E-MTAB-11583:Sample 59

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 59

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: Meloidogyne incognita

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 59


E-MTAB-11583:Sample 60

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 60

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: Meloidogyne incognita

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 60


E-MTAB-11583:Sample 21

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 21

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 21


E-MTAB-11583:Sample 22

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 22


E-MTAB-11583:Sample 23

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 23

text: ArrayExpress

text: thale cress

characteristic: common name

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 23

characteristic: title


E-MTAB-11583:Sample 24

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 24

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 24


E-MTAB-11583:Sample 37

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 37

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 37


E-MTAB-11583:Sample 38

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 38

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 38


E-MTAB-11583:Sample 39

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 39

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 39


E-MTAB-11583:Sample 40

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 40

text: ArrayExpress

text: thale cress

characteristic: common name

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 40

characteristic: title


E-MTAB-11583:Sample 5

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 5

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 5


E-MTAB-11583:Sample 53

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 53

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 53


E-MTAB-11583:Sample 54

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 54

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 54


E-MTAB-11583:Sample 55

text: Wageningen University and Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11583:Sample 55

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: description

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 55


E-MTAB-11583:Sample 56

characteristic: ENA-FIRST-PUBLIC

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 56

text: ArrayExpress

text: thale cress

characteristic: common name

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 56

characteristic: title


E-MTAB-11583:Sample 6

characteristic: ENA-LAST-UPDATE

text: Wageningen University and Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11583:Sample 6

characteristic: age

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 6


E-MTAB-11583:Sample 7

text: Wageningen University and Research

text: public

text: E-MTAB-11583:Sample 7

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: ecotype

text: opr2-1 mutant

characteristic: genotype

text: mock

characteristic: infect

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: Arabidopsis thaliana

text: Sample 7


E-MTAB-11583:Sample 8

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Wageningen University and Research

characteristic: INSDC last update

text: public

text: E-MTAB-11583:Sample 8

text: ArrayExpress

text: thale cress

text: opr2-1 mutant

text: mock

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

characteristic: organism part

text: Arabidopsis thaliana

text: Sample 8


E-MTAB-11587:Sample 1

text: Department of Life Sciences, POSTECH

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11587:Sample 1

tag: Namespace:Department of Life Sciences, POSTECH

characteristic: Submitter Id

unit: week

text: ArrayExpress

text: stomach epithelial cell

characteristic: cell type

text: house mouse

text: Protocols: Stomachs were prepared by carefully separating the corpus from the forestomach and pylorus. Corpus tissue was divided into four pieces of similar size and incubated in 4 ml dissociation solution (45 U/ml Dispase II, Thermo Fisher Scientific; 0.6 mg/ml pancreatin, Sigma; 1x penicillin/streptomycin in DMEM high glucose, HEPES, no phenol red, Thermo Fisher Scientific) at 37 °C with shaking at 270 rpm. After dissociation, the solution becomes cloudy and corpus fragments appear more transparent. All subsequent steps were then performed on ice. To avoid cell loss due to cell adhesion to pipette or tube walls, all pipettes and tubes used for pipetting the cell suspension were pre-incubated with either DMEM or PBS containing FBS. To further disrupt the tissue, the cell suspension, including corpus pieces, was pipetted up and down several times using a 10 ml pipette. The cell suspension without remaining corpus pieces was then transferred to a 15 ml Falcon tube. The corpus pieces and the dissociation tube were then washed twice with 5 ml of 10% FBS in DMEM and the wash solution combined with the cell suspension for inactivation of the dissociation reaction. Cells were centrifuged at 300 g for 5 min and resuspended in 1% FBS in PBS. The cell suspension was filtered through a 100 μm cell strainer into a pre-coated 15 ml tube. The tube and filters were washed twice with 1 ml of 1% FBS in PBS and the cell suspension was then centrifuged a second time at 300 g for 5 min. For scRNA-seq for Gif lineage cells, we utilized Gif-rtTA; TetO-Cre; Rosa-nTnG mice as described above. We administrated doxycycline by drinking water as described for animal treatments. Stomach samples from uninjured or DMP-777-treated mice at 2 dpi were collected separately and cells were dissociated. To sort GFP+ DsRed- cells only, we used WT stomach cells as a control to identify GFP+ DsRed+ cell windows in the FACS plot. The cells were sorted in 384-well plates. Plates were immediately frozen at -80 °C until they were shipped in dry ice to Single Cell Discoveries. Cells were lysed by 5 min at 65°C, after which RT and second strand mixes were dispersed with the Nanodrop II liquid handling platform. Aqueous phase was separated from the oil phase after pooling all cells in one library, followed by IVT transcription. The CEL-Seq2 protocol was used for library prep. Primers consisted of a 24 bp polyT stretch, a 4bp random molecular barcode (UMI), a cell-specific 8bp barcode, the 5′ Illumina TruSeq small RNA kit adaptor and a T7 promoter. mRNA of each cell was then reverse transcribed, converted to double-stranded cDNA, pooled and in vitro transcribed for linear following the CEL-Seq 2 protocol (Hashimshony et al., 2016).

characteristic: description

text: adult

characteristic: developmental stage

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

text: TdTom- GFP+

characteristic: immunophenotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: stomach

text: Mus musculus

text: male

characteristic: sex

text: doxycycline

characteristic: stimulus

text: Sample 1


E-MTAB-11587:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Life Sciences, POSTECH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11587:Sample 2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stomach epithelial cell

text: house mouse

characteristic: common name

text: adult

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

text: TdTom- GFP+

text: Mus musculus

characteristic: organism

text: Mus musculus

text: stomach

text: Mus musculus

text: male

text: doxycycline

text: Sample 2

characteristic: title


E-MTAB-11587:Sample 3

characteristic: ENA-CHECKLIST

characteristic: External Id

text: Department of Life Sciences, POSTECH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11587:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: stomach epithelial cell

text: house mouse

text: adult

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

characteristic: genotype

text: TdTom- GFP+

text: Mus musculus

text: Mus musculus

text: stomach

characteristic: organism part

text: Mus musculus

text: male

text: doxycycline

text: Sample 3


E-MTAB-11587:Sample 4

characteristic: ENA-FIRST-PUBLIC

text: Department of Life Sciences, POSTECH

text: public

text: E-MTAB-11587:Sample 4

text: ArrayExpress

text: stomach epithelial cell

characteristic: cell type

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

text: TdTom- GFP+

characteristic: immunophenotype

characteristic: individual

text: gastric parietal cell atrophy

characteristic: injury

text: Mus musculus

text: Mus musculus

text: stomach

text: Mus musculus

text: male

characteristic: sex

text: doxycycline

characteristic: stimulus

text: Sample 4


E-MTAB-11587:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Department of Life Sciences, POSTECH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11587:Sample 5

characteristic: age

text: ArrayExpress

characteristic: broker name

text: stomach epithelial cell

text: house mouse

characteristic: common name

text: adult

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

text: TdTom- GFP+

text: gastric parietal cell atrophy

text: Mus musculus

characteristic: organism

text: Mus musculus

text: stomach

text: Mus musculus

text: male

text: doxycycline

text: Sample 5

characteristic: title


E-MTAB-11587:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Department of Life Sciences, POSTECH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11587:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: stomach epithelial cell

characteristic: cell type

text: house mouse

text: adult

text: Gif-rtTA; TetO-Cre; Rosa-mTmG

characteristic: genotype

text: TdTom- GFP+

text: gastric parietal cell atrophy

text: Mus musculus

text: Mus musculus

text: stomach

characteristic: organism part

text: Mus musculus

text: male

text: doxycycline

text: Sample 6


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Zhiyao Mao

text: public

tag: Namespace:Zhiyao Mao

text: potato

text: W+OS-treated potato leaf;W+OS2 (reuploaded)

characteristic: description

text: Solanum tuberosum

text: Solanum tuberosum

text: W+OS


text: Robert Koch Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Random sampling

characteristic: Sampling Strategy

text: IMS-10334-CVDP-9EAFF36C-7B93-4EBD-89FD-479C53E8159E

tag: Namespace:Robert Koch Institute

text: RKI-OSEDA

text: Carl-Thiem-Klinikum Cottbus - Zentrum für Laboratoriumsmedizin Mikrobiologie und Krankenhaushygiene, Thiemstr. 111; 03048 Cottbus

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Germany

characteristic: geographic location (country and/or sea)

text: Europe/Germany/Brandenburg

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Molecular surveillance of SARS-CoV-2 in Germany


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Robert Koch Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Random sampling

text: IMS-10334-CVDP-4B38F4E6-6FC8-4E46-9D97-22AD31E24EB0

characteristic: Submitter Id

text: RKI-OSEDA

characteristic: broker name

text: not provided

text: Germany

text: Europe/Germany/Brandenburg

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

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