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text: house mouse

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text: KAROLINSKA INSTITUTET

text: public

tag: Namespace:KAROLINSKA INSTITUTET

text: malaria parasite P. falciparum

text: Venous blood samples were collected in Nyamisati, Tanzania, in 1999 where a longitudinal population has been followed for 25 years. 96 individuals were included, 80 previously shown to be qPCR positive for P. falciparum malaria and 16 who tested negative by qPCR using the multiplexed TaqMan method for Plasmodium species identification previously described. This study was approved by the Nyamisati Village Board and the Ethical Review Board of the National Institute for Medical Research in Tanzania. Packed cells in EDTA were used for DNA extractions.

characteristic: dev stage

characteristic: host scientific name

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text: Plasmodium falciparum

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text: 3D71_100000HB3_culture_lib2_Plate2_H9.fastq


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text: 3D7100000_1HB3_culture_lib2_Plate2_H1.fastq


characteristic: ENA-LAST-UPDATE

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text: KAROLINSKA INSTITUTET

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characteristic: SRA accession

text: malaria parasite P. falciparum

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text: 3D71_10000HB3_culture_lib2_Plate2_G9.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

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text: Plasmodium falciparum

text: Plasmodium falciparum


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

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text: 3D71000_1HB3_culture_lib2_Plate2_G2.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: description

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text: Plasmodium falciparum

text: 3D71000000_1HB3_culture_lib2_Plate2_G1.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: 3D7100_1HB3_culture_lib2_Plate2_F5.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: 3D71_100HB3_culture_lib2_Plate2_E8.fastq

characteristic: title


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text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

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text: KAROLINSKA INSTITUTET

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text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

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text: Plasmodium falciparum

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text: KAROLINSKA INSTITUTET

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text: KAROLINSKA INSTITUTET

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text: KAROLINSKA INSTITUTET

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text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

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text: KAROLINSKA INSTITUTET

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characteristic: External Id

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text: KAROLINSKA INSTITUTET

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text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_1_in_100000__V6_1_in_100000_synthetic_lib5_Plate1_C12.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_1_in_100000__V8_1_in_100000_synthetic_lib5_Plate1_D12.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V9_1_in_100000__V10_1_in_100000_synthetic_lib5_Plate1_E12.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V1-10_1_in_100000_synthetic_lib5_Plate1_F12.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Negative control PCR library construction

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum


text: KAROLINSKA INSTITUTET

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neatV10_1_1_in_100000_synthetic_lib5_Plate2_A12.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6_neatV2_3_1_in_100000_synthetic_lib5_Plate2_B12.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neatV4_5_1_in_100000_synthetic_lib5_Plate2_C12.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neatV6_7_1_in_100000_synthetic_lib5_Plate2_D12.fastq


characteristic: ENA-LAST-UPDATE

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Venous blood samples were collected in Nyamisati, Tanzania, in 1999 where a longitudinal population has been followed for 25 years. 96 individuals were included, 80 previously shown to be qPCR positive for P. falciparum malaria and 16 who tested negative by qPCR using the multiplexed TaqMan method for Plasmodium species identification previously described. This study was approved by the Nyamisati Village Board and the Ethical Review Board of the National Institute for Medical Research in Tanzania. Packed cells in EDTA were used for DNA extractions.

text: Plasmodium falciparum

text: Plasmodium falciparum


characteristic: External Id

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V10_neat_V8_9_1_in_100000_synthetic_lib5_Plate2_F12.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neat_V1-10_neat_synthetic_lib5_Plate3_A1.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neat_V1-10_1_in_10_synthetic_lib5_Plate3_A2.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neat_V1-10_1_in_100_synthetic_lib5_Plate3_A3.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: description

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_A4.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: V7_neat_V1-10_1_in_10000_synthetic_lib5_Plate3_A5.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7_neat_V1-10_1_in_100000_synthetic_lib5_Plate3_A6.fastq

characteristic: title


characteristic: ENA-CHECKLIST

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7-9_neatV1-10_neat_synthetic_lib5_Plate3_A7.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7-9_neatV1-10_1_in_10_synthetic_lib5_Plate3_A8.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7-9_neatV1-10_1_in_100_synthetic_lib5_Plate3_A9.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V7-9_neatV1-10_1_in_1000_synthetic_lib5_Plate3_A10.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum


text: KAROLINSKA INSTITUTET

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_1_in_10_synthetic_lib5_Plate4_A2.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_1_in_100_synthetic_lib5_Plate4_A3.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_1_in_1000_synthetic_lib5_Plate4_A4.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_1_in_10000_synthetic_lib5_Plate4_A5.fastq


characteristic: ENA-LAST-UPDATE

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_1_in_100000_synthetic_lib5_Plate4_A6.fastq


characteristic: External Id

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_V1-10_1_in_10_synthetic_lib5_Plate4_A7.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_V1-10_1_in_100_synthetic_lib5_Plate4_A8.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_V1-10_1_in_1000_synthetic_lib5_Plate4_A9.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_V1-10_1_in_10000_synthetic_lib5_Plate4_A10.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: description

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neat_V1-10_neat_synthetic_lib5_Plate3_B1.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: V8_neat_V1-10_1_in_10_synthetic_lib5_Plate3_B2.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neat_V1-10_1_in_100_synthetic_lib5_Plate3_B3.fastq

characteristic: title


characteristic: ENA-CHECKLIST

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_B4.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neat_V1-10_1_in_10000_synthetic_lib5_Plate3_B5.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V8_neat_V1-10_1_in_100000_synthetic_lib5_Plate3_B6.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V1-3_neat_V1-10_neat_synthetic_lib5_Plate3_B7.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V1-3_neatV1-10_1_in_10_synthetic_lib5_Plate3_B8.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V1-3_neatV1-10_1_in_100_synthetic_lib5_Plate3_B9.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V1-3_neatV1-10_1_in_1000_synthetic_lib5_Plate3_B10.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: KAROLINSKA INSTITUTET

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_1_in_10_synthetic_lib5_Plate4_B2.fastq


characteristic: ENA-LAST-UPDATE

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_1_in_100_synthetic_lib5_Plate4_B3.fastq


characteristic: External Id

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_1_in_1000_synthetic_lib5_Plate4_B4.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_1_in_10000_synthetic_lib5_Plate4_B5.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_1_in_100000_synthetic_lib5_Plate4_B6.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_neat_V1-10_1_in_10_synthetic_lib5_Plate4_B7.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: description

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_neat_V1-10_1_in_100_synthetic_lib5_Plate4_B8.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: C2_neat_V1-10_1_in_1000_synthetic_lib5_Plate4_B9.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C2_neat_V1-10_1_in_10000_synthetic_lib5_Plate4_B10.fastq

characteristic: title


characteristic: ENA-CHECKLIST

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neatV1-10_neat_synthetic_lib5_Plate3_C1.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_V1-10_1_in_10_synthetic_lib5_Plate3_C2.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_V1-10_1_in_100_synthetic_lib5_Plate3_C3.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_C4.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_V1-10_1_in_10000_synthetic_lib5_Plate3_C5.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_V1-10_1_in_100000_synthetic_lib5_Plate3_C6.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V4-6_neat_V1-10_neat_synthetic_lib5_Plate3_C7.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V4-6_neat_V1-10_1_in_10_synthetic_lib5_Plate3_C8.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V4-6_neat_V1-10_1_in_100_synthetic_lib5_Plate3_C9.fastq


characteristic: ENA-LAST-UPDATE

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V4-6_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_C10.fastq


characteristic: External Id

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_neatC2_neat_neat_synthetic_lib5_Plate4_C1.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_C2_1_in_10_synthetic_lib5_Plate4_C2.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_C2_1_in_100_synthetic_lib5_Plate4_C3.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_C2_1_in_1000_synthetic_lib5_Plate4_C4.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: description

text: Plasmodium falciparum

text: Plasmodium falciparum

text: C1_C2_1_in_10000_synthetic_lib5_Plate4_C5.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: V5_neat_C2_1_in_10_synthetic_lib5_Plate4_C7.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_C2_1_in_100_synthetic_lib5_Plate4_C8.fastq

characteristic: title


characteristic: ENA-CHECKLIST

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_C2_1_in_1000_synthetic_lib5_Plate4_C9.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V5_neat_C2_1_in_10000_synthetic_lib5_Plate4_C10.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6_neatV1-10_neat_synthetic_lib5_Plate3_D1.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

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text: V6_neat_V1-10_1_in_10_synthetic_lib5_Plate3_D2.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6_neat_V1-10_1_in_100_synthetic_lib5_Plate3_D3.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC center name

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_D4.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC first public

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

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text: V6_neat_V1-10_1_in_10000_synthetic_lib5_Plate3_D5.fastq


text: KAROLINSKA INSTITUTET

characteristic: INSDC last update

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6_neat_V1-10_1_in_100000_synthetic_lib5_Plate3_D6.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V2_neat_V3-6_neat_synthetic_lib5_Plate3_D7.fastq


characteristic: ENA-LAST-UPDATE

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V2_neat_V3-6_1_in_10_synthetic_lib5_Plate3_D8.fastq


characteristic: External Id

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V2_neat_V3-6_1_in_100_synthetic_lib5_Plate3_D9.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: SRA accession

text: malaria parasite P. falciparum

text: Plasmodium falciparum

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text: V2_neat_V3-6_1_in_1000_synthetic_lib5_Plate3_D10.fastq


text: KAROLINSKA INSTITUTET

text: public

characteristic: Submitter Id

text: malaria parasite P. falciparum

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text: V6-10_neat_C2_1_in_10_synthetic_lib5_Plate4_D7.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

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text: V6-10_neat_C2_1_in_100_synthetic_lib5_Plate4_D8.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination

characteristic: description

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V6-10_neat_C2_1_in_1000_synthetic_lib5_Plate4_D9.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: V6-10_neat_C2_1_in_10000_synthetic_lib5_Plate4_D10.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_neat_synthetic_lib5_Plate3_E1.fastq

characteristic: title


characteristic: ENA-CHECKLIST

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_1_in_10_synthetic_lib5_Plate3_E2.fastq


characteristic: ENA-FIRST-PUBLIC

text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_1_in_100_synthetic_lib5_Plate3_E3.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: dev stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_1_in_1000_synthetic_lib5_Plate3_E4.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: host scientific name

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_1_in_10000_synthetic_lib5_Plate3_E5.fastq


text: KAROLINSKA INSTITUTET

text: public

text: malaria parasite P. falciparum

characteristic: isolation source

text: Plasmodium falciparum

text: Plasmodium falciparum

text: V3_4_neat_V1-10_1_in_100000_synthetic_lib5_Plate3_E6.fastq