COG-UK/NORT-YYIRHA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIRHA

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIRHA

characteristic: title

text: not provided


status: PUBLIC

tag: attribute

text: Quadram Institute Bioscience

text: public

tag: Namespace:Quadram Institute Bioscience

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Quadram Institute Bioscience

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

characteristic: title

text: not provided


COG-UK/NORT-YYIHAE

text: Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

text: public

text: COG-UK/NORT-YYIHAE

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIHAE

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIUKQ

text: public

text: COG-UK/NORT-YYIUKQ

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIUKQ

characteristic: title

text: not provided


COG-UK/NORT-YYICOE

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYICOE

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYICOE

text: not provided


COG-UK/NORT-YYIJXQ

text: public

text: COG-UK/NORT-YYIJXQ

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYIJXQ

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIMHT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIMHT

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIMHT

characteristic: title

text: not provided


COG-UK/NORT-YYIFGR

text: public

text: COG-UK/NORT-YYIFGR

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIFGR

text: not provided


COG-UK/NORT-YYAZUT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAZUT

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAZUT

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIUBY

text: public

text: COG-UK/NORT-YYIUBY

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIUBY

characteristic: title

text: not provided


COG-UK/NORT-YYAOJP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAOJP

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAOJP

text: not provided


COG-UK/NORT-YYICRB

text: public

text: COG-UK/NORT-YYICRB

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYICRB

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYAZFQ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAZFQ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYAZFQ

characteristic: title

text: not provided


COG-UK/NORT-YYIOQJ

text: public

text: COG-UK/NORT-YYIOQJ

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIOQJ

text: not provided


COG-UK/NORT-YYSOMP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYSOMP

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYSOMP

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIUMC

text: public

text: COG-UK/NORT-YYIUMC

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIUMC

characteristic: title

text: not provided


COG-UK/NORT-YYIZEQ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIZEQ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIZEQ

text: not provided


COG-UK/NORT-YYAMQE

text: public

text: COG-UK/NORT-YYAMQE

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAMQE

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Quadram Institute Bioscience

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

characteristic: title

text: not provided


text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: not provided


COG-UK/NORT-YYIGDJ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIGDJ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYIGDJ

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIGTC

text: public

text: COG-UK/NORT-YYIGTC

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIGTC

characteristic: title

text: not provided


COG-UK/NORT-YYIDBO

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIDBO

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIDBO

text: not provided


COG-UK/NORT-YYWHNZ

text: public

text: COG-UK/NORT-YYWHNZ

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYWHNZ

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIWQF

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIWQF

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIWQF

characteristic: title

text: not provided


COG-UK/NORT-YYIBQA

text: public

text: COG-UK/NORT-YYIBQA

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIBQA

text: not provided


COG-UK/NORT-YYIDXW

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIDXW

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYIDXW

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIPGH

text: public

text: COG-UK/NORT-YYIPGH

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIPGH

characteristic: title

text: not provided


COG-UK/NORT-YYIEQT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIEQT

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYIEQT

text: not provided


COG-UK/NORT-YYIJBK

text: public

text: COG-UK/NORT-YYIJBK

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYIJBK

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYWZWZ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYWZWZ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYWZWZ

characteristic: title

text: not provided


COG-UK/NORT-YYISFI

text: public

text: COG-UK/NORT-YYISFI

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYISFI

text: not provided


COG-UK/NORT-YYAKPM

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAKPM

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAKPM

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYAEBF

text: public

text: COG-UK/NORT-YYAEBF

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYAEBF

characteristic: title

text: not provided


COG-UK/NORT-YYWZEO

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYWZEO

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYWZEO

text: not provided


COG-UK/NORT-YYARTE

text: public

text: COG-UK/NORT-YYARTE

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYARTE

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: Quadram Institute Bioscience

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

characteristic: title

text: not provided


text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: not provided


COG-UK/NORT-YYANCI

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYANCI

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYANCI

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYAUYH

text: public

text: COG-UK/NORT-YYAUYH

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYAUYH

characteristic: title

text: not provided


COG-UK/NORT-YYAPSI

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAPSI

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAPSI

text: not provided


COG-UK/NORT-YYARIY

text: public

text: COG-UK/NORT-YYARIY

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYARIY

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYWHDI

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYWHDI

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYWHDI

characteristic: title

text: not provided


COG-UK/NORT-YYAZJG

text: public

text: COG-UK/NORT-YYAZJG

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAZJG

text: not provided


COG-UK/NORT-YYICPX

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYICPX

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYICPX

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIRQI

text: public

text: COG-UK/NORT-YYIRQI

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIRQI

characteristic: title

text: not provided


COG-UK/NORT-YYWAPF

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYWAPF

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYWAPF

text: not provided


COG-UK/NORT-YYAKTN

text: public

text: COG-UK/NORT-YYAKTN

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAKTN

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYIMJA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYIMJA

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYIMJA

characteristic: title

text: not provided


COG-UK/NORT-YYAPOB

text: public

text: COG-UK/NORT-YYAPOB

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAPOB

text: not provided


COG-UK/NORT-YYAKHC

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAKHC

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAKHC

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYAMBN

text: public

text: COG-UK/NORT-YYAMBN

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYAMBN

characteristic: title

text: not provided


COG-UK/NORT-YYAHUC

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAHUC

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAHUC

text: not provided


text: Quadram Institute Bioscience

text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: Quadram Institute Bioscience

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

characteristic: title

text: not provided


COG-UK/NORT-YYAGAA

text: public

text: COG-UK/NORT-YYAGAA

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYAGAA

text: not provided


COG-UK/NORT-YYAZIP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYAZIP

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYAZIP

text: not provided

characteristic: virus identifier


COG-UK/NORT-YYAKEI

text: public

text: COG-UK/NORT-YYAKEI

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: receipt date

text: not provided

text: COG-UK/NORT-YYAKEI

characteristic: title

text: not provided


COG-UK/NORT-YYWHJJ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/NORT-YYWHJJ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: COG-UK/NORT-YYWHJJ

text: not provided


COG-UK/NORT-YYASAE

text: public

text: COG-UK/NORT-YYASAE

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/NORT-YYASAE

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Imagine Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Imagine Institute

characteristic: Submitter Id

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

characteristic: title


text: Wellcome Sanger Institute

text: public

tag: Namespace:Wellcome Sanger Institute

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: cholangiocyte like cell

characteristic: cell type

text: human

text: Protocols: A1ATD primary paper here: 10.1172/JCI43122. Related paper for additional information here: 10.1038/nature10424. Primary human adult liver tissue was obtained from deceased donors that have been rejected for transplantation. The tissue was stored at 4 C in organ preservation media after being removed from the patient, and subsequently washed with PBS+EDTA and incubated in HepatoZYME media (Thermo Fisher Scientific) supplemented with 0.2 Wünsch units/ml of Liberase DL (Sigma-Aldrich) and 1,000 U/ml DNase I (Sigma-Aldrich) on a shaker at 200 rpm, 37 C for 30 minutes to digest the ECM of the tissue. The tissue was passed through two cycles of the GentleMACS tissue dissociator (Miltenyi Biotech), after which PBS with 20% BSA was added to halt the enzymatic reaction. The hepatocytes were centrifuged at 50 x g, 4 C for 5 minutes, followed by a percoll gradient centrifugation to remove debris and dead cells and a red blood cell lysis step before final resuspension in HepatoZYME media. The non-parenchymal cell-containing supernatant was centrifuged sequentially at 300 x g and 650 x g, with both pellets being pooled. This fraction was cleaned using a debris removal kit (Miltenyi Biotech), followed by red blood cell lysis (Miltenyi Biotech) and resuspension in HepatoZYME media for barcoding and sequencing. Primary human adult cholangiocytes were collected by dissociating primary adult human liver, and passing the cell suspension through MACS beads to select EPCAM+ cells. These cells were resuspended in supplemented William’s E media for barcoding and sequencing. Primary human adult hepatocytes were collected from a collaborating lab using a method of enzymatic perfusion to isolate the cells. Subsequent clean-up steps were performed before barcoding and sequencing. Primary human fetal tissue was obtained from patients undergoing elective terminations (REC-96/085) in the first trimester. The liver was dissected from the abdominal cavity and placed into a solution containing Hanks Buffered Saline Solution (HBSS) supplemented with 1.07 Wünsch units/ml Liberase DH (Roche Applied Science) and 70 U/ml Hyaluronidase (Sigma-Aldrich), and placed on a microplate shaker at 37 C, 750 rpm, for 15 minutes. The sample was subsequently washed three times in HBSS using centrifugation at 400g for five minutes each time. The single cell suspension could then be sorted for EPCAM+/CD326+ cells using CD326 microbeads (Miltenyi Biotech), according to the manufacturer’s guidelines. The single cell suspension was resuspended in hepatoblast organoid media (HO-M) for barcoding and sequencing. Alternatively, primary human fetal tissue was digested and by a collaborating lab using enzymatic digestion and intermittent shaking. The resulting cell suspension was filtered and the red blood cells were lysed prior to resuspension for barcoding and sequencing. Many samples were sorted for CD45+ to separate the immune compartment for sequencing. Human induced pluripotent stem cells (hiPSC) were differentiated into hepatocyte-like cells, cholangiocyte-like cells, hepatic stellate-like cells, endothelial-like cells and macrophage-like cells following respective small molecule-driven differentiation protocols. These cells were detached from their plates in culture using common dissociation reagents including Accutase (Life Technologies), TrypLE Express (Life Technologies), Cell Dissociation Buffer (Thermo Fisher Scientific), and EDTA (Life Technologies) and resuspended in their respective medias for barcoding and sequencing. Hepatocyte-like cells were transduced with lentivirus at days 15 and 25 of the differentiation protocol and dissociated for sequencing on days 23-25 and 33 respectively. The cells were dissociated from culture using TryplE for 20 minutes at 37 C followed by accutase for 4 minutes at 37 C. The cells were centrifuged and resuspended in HepatoZYME media for barcoding and sequencing. Cell culture maintenance, passaging and differentiation Human iPS cells were maintained in defined culture conditions, using Essential 8 (e8) media (Gibco A1517001). The cells were incubated at 37 C and 5% CO2 with media changed daily. Typically, cells were maintained for two weeks before passaging. To passage the cells, PBS was used to wash followed by incubation at 37 C with 0.5 mM EDTA in PBS for 4 mins. The PBS + 0.5mM EDTA was aspirated, fresh e8 media was added, and a pipette was used to flush the wells to detach the cells and break them into 5-10 cell clumps. The cells were transferred into a tube and allowed to settle, thus eliminating the small clumps and single cells. The supernatant was aspirated, the pellet was resuspended in complete e8 media and plated, typically in a 1:12 ratio split. The differentiation of hiPSCs to HLCs followed the protocol previously published by the Vallier lab. Prior to differentiation, 12-well plates were coated with 0.1% gelatin for an hour at 37 C followed by MEF media overnight at 37 C and washed with PBS before use. Confluent hiPSC wells were washed with PBS before adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 C for 4 mins. After incubation, the Accutase was diluted at a 1:1 ratio with complete e8 media (Gibco A1517001) and the supernatant was pipetted gently to mechanically break any clumps into single cells. The resulting suspension was centrifuged at ~350 x g for 3 mins to pellet, resuspended in e8 media and counted. The hiPSCs were added to complete e8 media supplemented with Y27632 ROCK inhibitor (10 mM) and plated at a density of 50,000-60,000 cells/cm2 in 12-well plates. The media was changed the following day to complete e8 without ROCK inhibitor. On day 1 of differentiation (2 days after plating), CDM-PVA media supplemented with activin (100 ng/ml), FGF2 (80 ng/ml), BMP4 (10 ng/ml), LY294002 (10 mM), and CHIR99021 (3 µM) was added to the cells to induce endoderm formation. This same media without CHIR99021 was used on day 2 of differentiation. On day 3, RPMI-1640 with nonessential amino acids (ThermoFisher 11140050) and B27 supplement (ThermoFisher 17504044) was supplemented with activin (100 ng/ml) and FGF2 (80 ng/ml). Foregut differentiation was initiated on day 4 and carried out until day 8 by changing media to complete RPMI media supplemented with activin (50 ng/ml). The hepatoblast and subsequent hepatocyte phenotype was induced by changing media to HepatoZYME-SFM (Life Technologies 17705-021) supplemented with nonessential amino acids, chemically defined lipid concentrate (ThermoFisher 11905031), L-glutamine (2 mM), insulin (14 ng/ml), transferrin (10 ng/ml), oncostatin M (OSM) (20 ng/ml) and hepatocyte growth factor (HGF) (50 ng/ml) from day 9 to day 33. Lentiviral Overexpression Protocol. In these transcription factor overexpression experiments, hiPSC-derived hepatocyte-like cells were transduced on day 15 of differentiation and assayed on day 23/25, or transduced on day 25 of differentiation and assayed on day 33. On the day of transduction, complete hepatocyte-like cell differentiation media using basal HepatoZYME-SFM (ThermoFisher 17705021) was supplemented with OSM (20 ng/ml) HGF (50 ng/ml) and polybrene (10 μg/ml) and added to cells in a 12-well plate format. Lentiviral aliquots were thawed on ice and added to the desired treatment wells in sterile conditions. Plates were rocked gently to ensure even distribution of lentivirus on the adherent cells. The cells were incubated at 37 C for 24 hrs to allow the lentivirus to infect the cells to stably integrate its DNA into the host genome. After 24 hrs, the cells were washed with DPBS and fresh, complete HepatoZYME-SFM with OSM (20 ng/ml) and HGF (50 ng/ml) was added to the cells. This washing procedure was repeated again at 48 hrs and 72 hrs post-transduction to ensure the removal of any remaining lentivirus. Conversion was performed following the 10X protocol (https://www.10xgenomics.com/) Chromium 10X platform. Constructed by core facility at Wellcome Sanger Institute.

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tag: Namespace:MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: Submitter Id

characteristic: collection date

text: Seawater (North Sea) was filtered through a 10um and then a 3um filter. The filtrate was diluted and plated on a 2216 agar plate. The colony forming units were harvested and sequenced

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial feature [ENVO:01000007]

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: North Sea

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: uncultured marine microorganism

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text: Illumina HiSeq 2500, rapid mode, 2x 250 bp paired-end run

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text: marine biome [ENVO:00000447]

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characteristic: Submitter Id

characteristic: collection date

text: Seawater (North Sea) was filtered through a 10um filter. The retentate was detached from the filter using sterile artificial seawater. The 10um particle suspension was homogenized, diluted, and plated on a 2216 agar plate. The colony forming units were harvested and sequenced

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: microbial feature [ENVO:01000007]

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: North Sea

characteristic: geographic location (country and/or sea)

text: mimarks-survey

text: uncultured marine microorganism

text: uncultured marine microorganism

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: public

text: Seawater (North Sea) was filtered through a 10um and a 3um filter. The retentate on the 3um filter was detached using sterile artificial seawater. The 3um particle suspension was homogenized, diluted, and plated on a 2216 agar plate. The colony forming units were harvested and sequenced

text: marine biome [ENVO:00000447]

text: microbial feature [ENVO:01000007]

text: coastal sea water [ENVO:00002150]

text: North Sea

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: uncultured marine microorganism

characteristic: project name

text: uncultured marine microorganism

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: Scotland

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

characteristic: title

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: receipt date

text: not provided

text: not provided


text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Statens Serum Institut and the Danish Covid19 Genome Consortium (DCGC)

characteristic: Submitter Id

text: Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark

text: Danish Covid-19 Genome Consortium

text: Original

characteristic: description

text: Denmark

text: Syddanmark

text: Human

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


text: Statens Serum Institut and the Danish Covid19 Genome Consortium (DCGC)

text: public

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Nordjylland

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Syddanmark

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Hovedstaden

text: Human

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Hovedstaden

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-105739/2021

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Midtjylland

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Sjaelland

text: Human

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Nordjylland

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-11957/2020

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Hovedstaden

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-163411/2021

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Hovedstaden

text: Human

text: n_infections=2,last_infection_date=2021-06-07

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Midtjylland

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: n_infections=2,last_infection_date=2020-12-07

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Sjaelland

text: Human

text: n_infections=2,last_infection_date=2020-03-09

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Nordjylland

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: n_infections=2,last_infection_date=2020-12-28

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Midtjylland

text: Human

text: n_infections=2,last_infection_date=2020-11-16

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Syddanmark

text: Human

text: n_infections=2,last_infection_date=2021-01-25

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Syddanmark

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-135648/2021

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Nordjylland

text: Human

text: n_infections=2,last_infection_date=2021-01-04

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

characteristic: geographic location (country and/or sea)

text: Nordjylland

text: Human

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Syddanmark

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided