text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i503.HK3H3BBXY.s_2

tag: Namespace:Helmholtz Munich

text: 74 to 80

unit: week

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i721_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i721_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i721_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i721_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i721_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i721_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i722_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i722_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i722_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i722_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i722_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i722_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i722_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i722_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i722_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i722_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i722_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i723_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i723_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i723_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i723_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i723_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i723_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i723_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i723_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i723_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i723_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i723_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i724_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i724_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i724_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i724_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i724_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i724_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i724_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i724_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i724_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i724_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i724_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i726_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i726_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i726_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i726_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i726_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i726_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i726_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i726_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i726_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i726_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i726_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i727_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i727_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i727_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i727_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i727_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i727_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i727_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i727_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i727_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i727_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i727_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i728_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i728_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i728_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i728_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i728_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i728_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i728_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i728_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i728_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i728_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: Lin positive Kit positive

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i728_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i729_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i729_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i729_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i729_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i729_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i729_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: Lin positive Kit positive

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i729_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i729_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i729_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: hematopoietic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i729_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

text: Lin− Sca-1+ cKit+

text: hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i729_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: hematopoietic stem cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: Lin− Sca-1+ cKit+

characteristic: immunophenotype

text: hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Helmholtz Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 8 to 12

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: BMMNC were stained with conjugated monoclonal antibodies for HSPC markers as described above. 10,000 Lin-kit+ cells from young or aged WT/Sema4AKO mice were sorted in Ca2+/Mg2+-free phosphate-buffered saline (D-PBS) supplemented with 2% bovine serum albumin (New England Biolabs). Following one round of centrifugation, the cells were processed on 10x Genomics platform according to the manufacturer’s protocol. Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer’s protocol. Libraries from 10,000 Kit+ cells each from WT (n=1) and Sema4A KO (n=2) young and WT (n=2) old mice were prepared using the Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer’s protocol.

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: young

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:RWTH Aachen University Hospital

characteristic: collection date

text: Genome of pure isolate Acetatifactor_sp_nov_DSM110981 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Acetatifactor sp. DSM 110981

text: Enriched cultured diversity of the mouse gut microbiota for custom-made synthetic communities

characteristic: project name

text: Acetatifactor sp. DSM 110981

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Acetatifactor_sp_nov_DSM110981


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Adlercreutzia_sp_nov3_DSM108611 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Adlercreutzia sp. DSM 108611

characteristic: organism

text: Adlercreutzia sp. DSM 108611

text: Illumina NextSeq500

text: Assembly for Adlercreutzia_sp_nov3_DSM108611

characteristic: title


text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Adlercreutzia_sp_nov2_DSM109821 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Adlercreutzia sp. DSM 109821

text: Adlercreutzia sp. DSM 109821

text: Illumina NextSeq500

text: Assembly for Adlercreutzia_sp_nov2_DSM109821


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Adlercreutzia_sp_nov1_DSM112345 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Adlercreutzia sp. DSM 112345

characteristic: project name

text: Adlercreutzia sp. DSM 112345

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Adlercreutzia_sp_nov1_DSM112345


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Alistipes_sp_nov_DSM112343 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Alistipes sp. DSM 112343

characteristic: organism

text: Alistipes sp. DSM 112343

text: Illumina NextSeq500

text: Assembly for Alistipes_sp_nov_DSM112343

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Atopobiaceae_gen_nov2_DSM105314 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Caniella sp. DSM 105314

text: Caniella sp. DSM 105314

text: Illumina MiSeq

text: Assembly for Atopobiaceae_gen_nov2_DSM105314


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Atopobiaceae_gen_nov2_DSM110323 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Caniella sp. DSM 110323

characteristic: project name

text: Caniella sp. DSM 110323

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Atopobiaceae_gen_nov2_DSM110323


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Atopobiaceae_gen_nov2_DSM110983 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Caniella sp. DSM 110983

characteristic: organism

text: Caniella sp. DSM 110983

text: Illumina NextSeq500

text: Assembly for Atopobiaceae_gen_nov2_DSM110983

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Eggerthellaceae_gen_nov_CLA_AA_M13 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Anaerotardibacter sp. CLA_AA_M13

text: Anaerotardibacter sp. CLA_AA_M13

text: Illumina NextSeq500

text: Assembly for Eggerthellaceae_gen_nov_CLA_AA_M13


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Eubacteriaceae_gen_nov1_DSM106208 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Senimuribacter sp. DSM 106208

text: Enriched cultured diversity of the mouse gut microbiota for custom-made synthetic communities

characteristic: project name

text: Senimuribacter sp. DSM 106208

text: Illumina MiSeq

characteristic: sequencing method

text: Assembly for Eubacteriaceae_gen_nov1_DSM106208


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Eubacteriaceae_gen_nov1_DSM109599 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Senimuribacter sp. DSM 109599

characteristic: organism

text: Senimuribacter sp. DSM 109599

text: Illumina NextSeq500

text: Assembly for Eubacteriaceae_gen_nov1_DSM109599

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Eubacteriales_fam_nov_CLA_AA_M08 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Pumilibacter sp. CLA_AA_M08

text: Pumilibacter sp. CLA_AA_M08

text: Illumina NextSeq500

text: Assembly for Eubacteriales_fam_nov_CLA_AA_M08


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Eubacteriales_fam_nov_CLA_AA_M10 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Pumilibacter sp. CLA_AA_M10

characteristic: project name

text: Pumilibacter sp. CLA_AA_M10

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Eubacteriales_fam_nov_CLA_AA_M10


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Eubacteriales_fam_nov_CLA_AA_M11 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Anaerocaecibacter sp. CLA_AA_M11

characteristic: organism

text: Anaerocaecibacter sp. CLA_AA_M11

text: Illumina NextSeq500

text: Assembly for Eubacteriales_fam_nov_CLA_AA_M11

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Lachnospiraceae_gen_nov1_DSM111138 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Otoolea sp. DSM 111138

text: Otoolea sp. DSM 111138

text: Illumina NextSeq500

text: Assembly for Lachnospiraceae_gen_nov1_DSM111138


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Lachnospiraceae_gen_nov3_DSM111139 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Hominisplanchenecus sp. DSM 111139

characteristic: project name

text: Hominisplanchenecus sp. DSM 111139

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Lachnospiraceae_gen_nov3_DSM111139


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Lactobacillus_sp_nov_DSM110155 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Lactobacillus sp. DSM 110155

characteristic: organism

text: Lactobacillus sp. DSM 110155

text: Illumina NextSeq500

text: Assembly for Lactobacillus_sp_nov_DSM110155

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: collection date

text: Genome of pure isolate Lactococcus_sp_nov2_DSM107391 cultured from mouse cecal content

text: mouse gastrointestinal tract

characteristic: environment (biome)

text: mouse intestine

characteristic: environment (feature)

text: gut content

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

text: host-associated

text: single amplified genome

text: Lactococcus sp. DSM 107391

text: Lactococcus sp. DSM 107391

text: Illumina NextSeq500

text: Assembly for Lactococcus_sp_nov2_DSM107391


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: RWTH Aachen University Hospital

text: public

text: Genome of pure isolate Lactococcus_sp_nov1_DSM109779 cultured from mouse cecal content

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: single amplified genome

characteristic: investigation type

text: Lactococcus sp. DSM 109779

text: Enriched cultured diversity of the mouse gut microbiota for custom-made synthetic communities

characteristic: project name

text: Lactococcus sp. DSM 109779

text: Illumina NextSeq500

characteristic: sequencing method

text: Assembly for Lactococcus_sp_nov1_DSM109779


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: RWTH Aachen University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Genome of pure isolate Limosilactobacillus_sp_nov2_DSM106037 cultured from mouse cecal content

characteristic: description

text: mouse gastrointestinal tract

text: mouse intestine

text: gut content

text: Germany

text: host-associated

text: single amplified genome

text: Limosilactobacillus sp. DSM 106037

characteristic: organism

text: Limosilactobacillus sp. DSM 106037

text: Illumina MiSeq

text: Assembly for Limosilactobacillus_sp_nov2_DSM106037

characteristic: title