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characteristic: geographic location (country and/or sea)

text: Midtjylland

characteristic: geographic location (region and locality)

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-145125/2021

text: not provided

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tag: attribute

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text: Original

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text: not provided

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text: active surveillance in response to outbreak

characteristic: sample capture status

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characteristic: ENA-CHECKLIST

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text: not provided


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text: public

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text: Nordjylland

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text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

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text: Sjaelland

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text: Human

text: n_infections=2,last_infection_date=2020-12-07

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text: public

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text: not provided

text: not provided

text: not provided

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text: active surveillance in response to outbreak


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text: public

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text: Danish Covid-19 Genome Consortium

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text: Danish Covid-19 Genome Consortium

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text: Syddanmark

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text: not provided


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

text: public

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text: Danish Covid-19 Genome Consortium

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text: not provided

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text: public

text: Danish Covid-19 Genome Consortium

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text: Sjaelland

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text: Human

text: not provided

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text: Danish Covid-19 Genome Consortium

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text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

characteristic: External Id

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text: public

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text: Denmark

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text: public

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text: Danish Covid-19 Genome Consortium

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text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-158744/2021

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text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Hovedstaden

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-104217/2021

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Midtjylland

text: Human

characteristic: host common name

text: n_infections=2,last_infection_date=2020-08-10

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Statens Serum Institut and the Danish Covid19 Genome Consortium (DCGC)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Hovedstaden

characteristic: geographic location (region and locality)

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-149049/2021

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Hovedstaden

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Nordjylland

text: Human

characteristic: host common name

text: n_infections=2,last_infection_date=2020-03-30

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Syddanmark

characteristic: geographic location (region and locality)

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-16481/2020

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Syddanmark

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-76604/2021

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Hovedstaden

text: Human

characteristic: host common name

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-49901/2020

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Hovedstaden

characteristic: geographic location (region and locality)

text: Human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Midtjylland

text: Human

text: n_infections=2,last_infection_date=2020-12-14

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Sjaelland

text: Human

characteristic: host common name

text: n_infections=2,last_infection_date=2020-05-18

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Syddanmark

characteristic: geographic location (region and locality)

text: Human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Hovedstaden

text: Human

text: n_infections=2,last_infection_date=2020-11-23

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Sjaelland

text: Human

characteristic: host common name

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Midtjylland

characteristic: geographic location (region and locality)

text: Human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Nordjylland

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

text: Hovedstaden

text: Human

characteristic: host common name

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-96711/2021

characteristic: host description

text: not provided

text: Homo sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: Danish Covid-19 Genome Consortium

text: Original

text: Denmark

characteristic: geographic location (country and/or sea)

text: Hovedstaden

characteristic: geographic location (region and locality)

text: Human

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: Danish Covid-19 Genome Consortium

characteristic: collector name

text: Original

characteristic: description

text: Denmark

text: Syddanmark

text: Human

text: n_infections=2,last_infection_id=hCoV-19/Denmark/DCGC-134961/2021

text: not provided

text: Homo sapiens

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:Finnish Institute for Health and Welfare

text: Vibrio cholerae WGS sample

text: Vibrio cholerae

text: Vibrio cholerae

characteristic: title


text: Finnish Institute for Health and Welfare

text: public

text: Vibrio cholerae WGS sample

text: Vibrio cholerae

text: Vibrio cholerae


text: Anthony Smith, NICD

text: public

tag: Namespace:Anthony Smith, NICD

text: EnteroBase

characteristic: broker name

text: EnteroBase

text: A. Smith, National Institute for Communicable Diseases

characteristic: collected by

text: Whole genome sequencing data uploaded by EnteroBase user

text: South Africa, Gauteng

characteristic: geographic location

text: Human, Stool

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Isangi

characteristic: serovar

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: EnteroBase

text: EnteroBase

text: South Africa, KwaZulu-Natal

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Typhi


characteristic: ENA-CHECKLIST

text: Anthony Smith, NICD

text: public

characteristic: Submitter Id

text: EnteroBase

text: EnteroBase

characteristic: collection date

characteristic: description

text: South Africa, Gauteng

text: Human, Blood culture

text: Salmonella enterica

text: Salmonella enterica

text: Typhi


characteristic: ENA-FIRST-PUBLIC

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: EnteroBase

text: EnteroBase

text: South Africa, Free State

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: sub species Salamae

characteristic: title


text: Anthony Smith, NICD

text: public

text: EnteroBase

characteristic: broker name

text: EnteroBase

characteristic: collected by

text: South Africa, Free State

characteristic: geographic location

text: Human, Stool

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

text: South Africa, Free State

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Virchow

characteristic: serovar

characteristic: strain


text: Anthony Smith, NICD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: EnteroBase

text: EnteroBase

text: South Africa, Free State

text: Human, Stool

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Typhimurium


characteristic: ENA-CHECKLIST

text: Anthony Smith, NICD

text: public

characteristic: Submitter Id

text: EnteroBase

text: EnteroBase

characteristic: collection date

characteristic: description

text: South Africa, Western Cape

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis


characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

tag: Namespace:Laboratory of Molecular Infection Medicine Sweden (MIMS), Umea university

unit: year

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Primary human bronchial epithelial cells (HBEC) were isolated with informed consent from lung tissue from of patients who underwent thoracic surgery. HBEC were grown in Bronchial Epithelial Cell Medium (BEpiCM, SC3211-b, SienCell) with recommended supplements (SC3262, SienCell) and 100 U PeSt. For differentiation 0.75 x105 cells were seeded onto a 6.5 mm semipermeable transwell insert (0.4 µm Pore Polyester Membrane Insert, Corning) in differentiation media (DMEM:BEpiCM 1:1 supplemented with 52 μg/ml bovine pituitary extract, 0.5 μg/ml hydrocortisone, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 10 μg/ml transferrin, 5 μg/ml insulin, 50 nM retinoic acid (all from Sigma Aldrich) and PeSt according to (doi: 10.1152/ajplung.00311.2001. PubMed PMID: 11792627). Cells were maintained submerged for the first 7 days, after which the media was removed from the apical side and cells were grown at air-liquid interface for an additional two weeks to reach full differentiation. Media was replaced three times per week with addition of fresh retinoic acid to the media shortly before usage. Immediately prior to infection the apical side of the HBEC ALI cultures was rinsed three times with PBS. For infection, 1.5x104 PFU of SARS-CoV-2 in a total volume of 100 µl infection medium (DMEM + 100 U PeSt) was added to the apical compartment, corresponding to an approximate MOI of 0.05. Cells were incubated at 37 °C and 5 % CO2 for 2.5 h before the inoculum was removed and the cells washed with PBS to remove residual medium. 72 h.p.i the cells were washed three times with PBS and RNA was harvested from mock-infected (n=1) and infected (n=2) HBEC from individual donors using the NucleoSpin RNA II kit (Macherey-Nagel) in accordance with the manufacturer’s instructions. According to smartseq2, https://www.nature.com/articles/nprot.2014.006

text: adult

characteristic: developmental stage

text: a

characteristic: individual

characteristic: infect

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratory of Molecular Infection Medicine Sweden (MIMS), Umea university

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: bronchial epithelial cell

text: human

text: adult

text: a

text: Severe acute respiratory syndrome coronavirus 2

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: ArrayExpress

text: bronchial epithelial cell

text: human

characteristic: description

text: adult

text: b

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


text: public

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: c

characteristic: individual

characteristic: infect

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

text: adult

text: e

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: bronchial epithelial cell

text: human

text: adult

text: f

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

characteristic: cell type

text: human

characteristic: description

text: adult

text: k

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: l

characteristic: individual

characteristic: infect

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

text: adult

text: m

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: bronchial epithelial cell

text: human

text: adult

text: d

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

characteristic: cell type

text: human

characteristic: description

text: adult

text: a

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: b

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

text: adult

text: c

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: bronchial epithelial cell

text: human

text: adult

text: d

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

characteristic: cell type

text: human

text: adult

text: e

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: f

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: bronchial epithelial cell

text: human

text: adult

text: k

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

characteristic: broker name

text: bronchial epithelial cell

text: human

text: adult

text: l

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: bronchial epithelial cell

text: human

text: adult

text: m

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male


E-MTAB-11682:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM

text: public

text: E-MTAB-11682:Sample 1

text: ArrayExpress

characteristic: cell line

text: human

characteristic: common name

text: Protocols: The SUM190 BC cell line was kindly provided by Dr Ethier (https://sumlineknowledgebase.com/) Tumor RNAs were extracted from sensitive and resistant pre-clinical models and controlled on Agilent Bioanalyzer (Agilent Technologies, Massy, France) cDNA was synthetized by random hexamer primers and reverse transcriptase (Superscript II, Life Technologies). Next, end repair and 3′ ends adenylation of the fragments were performed. Bar-coded adapters were ligated to the cDNA fragments and a PCR reaction was performed to produce the sequencing libraries. The quality check of libraries and quantification were performed using Tape station High sensitivity D1000

characteristic: description

text: breast cancer

characteristic: disease

text: Nectin-4 ADC resistant

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: female

text: Sample 1


E-MTAB-11682:Sample 2

text: INSERM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11682:Sample 2

text: ArrayExpress

text: human

text: breast cancer

text: Nectin-4 ADC resistant

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: Sample 2


E-MTAB-11682:Sample 3

characteristic: ENA-LAST-UPDATE

text: INSERM

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11682:Sample 3

text: ArrayExpress

characteristic: broker name

text: human

text: breast cancer

text: Nectin-4 ADC sensitive

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: female

text: Sample 3

characteristic: title


E-MTAB-11683:Sample 1

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-11683:Sample 1

tag: Namespace:Departments of Physiology and Medicine University of Toronto

characteristic: Submitter Id

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

characteristic: cell type

text: house mouse

text: Protocols: Total RNA was collected at 8 hr after synchronization scRNA vs siRNA Paris kit (ThermoFisher) Takara Smartseq V4

text: adult

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

characteristic: strain

text: Sample 1


E-MTAB-11683:Sample 2

characteristic: ENA-FIRST-PUBLIC

text: Departments of Physiology and Medicine University of Toronto

characteristic: INSDC center name

text: public

text: E-MTAB-11683:Sample 2

text: ArrayExpress

text: GLUTag

characteristic: cell line

text: type L enteroendocrine cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 2


E-MTAB-11683:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11683:Sample 3

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: CD1 mus strain

text: Sample 3


E-MTAB-11683:Sample 4

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11683:Sample 4

text: ArrayExpress

characteristic: broker name

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 4

characteristic: title


E-MTAB-11683:Sample 5

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-11683:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

characteristic: cell type

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 5


E-MTAB-11683:Sample 6

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11683:Sample 6

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

characteristic: strain

text: Sample 6


E-MTAB-11683:Sample 7

characteristic: INSDC center name

text: public

text: E-MTAB-11683:Sample 7

text: ArrayExpress

text: GLUTag

characteristic: cell line

text: type L enteroendocrine cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 7


E-MTAB-11683:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11683:Sample 8

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

characteristic: organism part

text: Mus musculus

text: CD1 mus strain

text: Sample 8


E-MTAB-11683:Sample 9

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11683:Sample 9

text: ArrayExpress

characteristic: broker name

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 9

characteristic: title


E-MTAB-11683:Sample 10

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-11683:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

characteristic: cell type

text: house mouse

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 10


E-MTAB-11683:Sample 11

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11683:Sample 11

text: ArrayExpress

text: GLUTag

text: type L enteroendocrine cell

text: house mouse

text: adult

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

characteristic: strain

text: Sample 11


E-MTAB-11683:Sample 12

characteristic: INSDC center name

text: public

text: E-MTAB-11683:Sample 12

text: ArrayExpress

text: GLUTag

characteristic: cell line

text: type L enteroendocrine cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Mus musculus

text: Mus musculus

text: colon

text: Mus musculus

text: CD1 mus strain

text: Sample 12


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:684-55158

tag: Namespace:VIB-UGent Center for Inflammation Research

text: house mouse

text: Untreated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 1

text: Mus musculus

text: Mus musculus


text: VIB-UGent Center for Inflammation Research

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55159

text: house mouse

text: Untreated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 2

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55160

text: house mouse

text: Untreated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 3

text: Mus musculus

text: Mus musculus

characteristic: title


characteristic: External Id

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55161

characteristic: Submitter Id

text: house mouse

text: Untreated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 1

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55162

text: house mouse

text: Untreated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 2

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55163

text: house mouse

text: Untreated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 3

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55164

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 1

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55165

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 2

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55166

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of Adar+/- x Mavs -/- mouse 3

text: Mus musculus

text: Mus musculus


text: VIB-UGent Center for Inflammation Research

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55167

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 1

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55168

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 2

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55169

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of AdarZa/- x Mavs -/- mouse 3

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55170

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of Adar-/- x Mavs -/- mouse 1

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:10:56:685-55171

text: house mouse

text: IFNa-B/D treated primary lung fibroblasts derived from of Adar-/- x Mavs -/- mouse 2

text: Mus musculus

text: Mus musculus


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:440-55173

text: human

text: IFNa-treated parental HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: Parental HEK293 cells


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:440-55174

text: human

text: IFNa-treated ADAR +/+ clone 1 HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: ADAR +/+ HEK293 clone 1


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:440-55175

text: human

text: IFNa-treated ADAR +/+ clone 2 HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: ADAR +/+ HEK293 clone 2


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:441-55176

text: human

text: IFNa-treated ADAR Za/Za clone 1 HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: ADAR Za/Za HEK293 clone 1


text: VIB-UGent Center for Inflammation Research

text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:441-55177

text: human

text: IFNa-treated ADAR Za/Za clone 2 HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: ADAR Za/Za HEK293 clone 2


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:441-55178

text: human

text: Homo sapiens

text: Homo sapiens

text: ADAR Za/Za HEK293 clone 3


text: public

text: ena-SAMPLE-TAB-04-05-2022-15:13:10:441-55179

text: human

text: IFNa-treated ADAR KO clone HEK293 cells

text: Homo sapiens

text: Homo sapiens

text: ADAR KO HEK293 clone


text: KAROLINSKA INSTITUTE

text: public

tag: Namespace:KAROLINSKA INSTITUTE

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: For Ribo-Seq, parental HEK293 and RF1 KO cells were treated with 100 g/ml chloramphenicol and 100 g/mL cycloheximide for 5 min. Cells were rinsed with 5 ml of ice-cold PBS, the dishes were submerged in a reservoir of liquid nitrogen for 10 s and transferred to dry ice. Cells were lysed in 400 l of lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1 % Triton X-100, 100 g/ml chloramphenicol and cycloheximide). Cells were scraped and lysates clarified by centrifugation for 20 min at 13,000 g at 4C. 200 l aliquots of cell lysates were treated with 7.5 l RNase 1 (100 units/l) at 28C. The reaction was layered onto a 10-30 % sucrose gradient [2ml 10-30% (v/v)] in 50mM Tris-HCl (pH7.2), 20mM Mg(OAc)2, 80mM NH4Cl, 0.1M KCl, 1mM PMSF and centrifuged for 2h 15min at 100,000 gmax at 4C (39,000 rpm, Beckman Coulter TLS-55 rotor) and fractions collected. Subsequently, all fractions containing mitochondrial monosomes, ascertained by western blotting, were pooled and digested with proteinase K (200 g/ml) for 30 min at 42C. RPFs were recovered by extracting twice with pre-warmed (65C) acidic phenol:chloroform and once with chloroform (1:1, v/v, buffered with 10 mM Tris pH 7.5, 0.1 mM EDTA) followed by ethanol precipitation. RPFs (1 g) were separated on 15 % denaturing polyacrylamide gels and RNA species migrating between 25 and 35 nt were harvested. RNA was eluted from the gel slices and ethanol precipitated. The RNA samples from above were heated at 80C for 2 min, cooled and the 3 phosphate group removed using T4 polynucleotide kinase (New England Biolabs) for 2 h at 37C in a 20 l reaction. The RNA was concentrated by ethanol precipitation, resuspended in 10 mM Tris-HCl (pH 7.5) and ligated in a 20 l reaction overnight at 14C to a preadenylated 3-adaptor using T4 RNA Ligase 2 (New England Biolabs). RNA was precipitated, loaded into a 15 % denaturing polyacrylamide gel and ligated RNA fragments migrating between 48 and 54 nt were excised. The RNA was eluted, precipitated, resuspended as above, and 5 phosphorylated using T4 PNK in the presence of 1 mM ATP for 2 h at 37C. RNA was concentrated by ethanol precipitation, resuspended in 10 mM Tris-HCl (pH 7.5) and ligated to a 5 RNA adaptor overnight at 14C using T4 RNA Ligase (New England Biolabs). The fully adapted RNAs were recovered by ethanol precipitation, dissolved in 6 l 10 mM Tris-HCl (pH 7.5), and ligated product annealed to an RT primer for 5 min at 65C. The RNA was subsequently reverse transcribed for 50 min at 55C in using SuperScript III according to manufacturers instructions, followed by heat inactivation for 5 min at 85C. Standard PCR reactions were used to prepare amplicons using forward primer RP1 and RPIX as reverse primer, where X is primer number (1-24). PCR reactions were loaded onto 10 % non-denaturing polyacrylamide-TBE gels and products of ~150 bp were excised from the gel and eluted. These amplicon libraries were ethanol precipitated and resuspended in 15 l 10 mM Tris-HCl (pH 7.5). Ribosomal RNA was depleted from Ribo-seq samples at the library amplicon stage; 12 L of the relevant Ribo-seq library was mixed with 4 L of 4 hybridization buffer (200 mM HEPES pH 7.5 and 2 M NaCl) and denatured at 98C for 2 min. DNA was re-annealed for 5 h at 68C prior to addition of 2 L of 10 DSN master buffer and 2 L of DSN (4 units, Evrogen). Digestion was allowed to proceed for 25 min at 68C, before addition of 20 L 10 mM EDTA and incubation for a further 5 min at 68C. DNA was recovered by a single extraction with phenolchloroform (1:1, vol/vol) followed by ethanol precipitation and resuspended in 4 L 10 mM TrisHCl pH 7.5. The treated amplicon library was subjected to another round of PCR (as above) and the resulting library subjected to a second round of DSN treatment.

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens


text: KAROLINSKA INSTITUTE

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: cell line

text: human

characteristic: common name

text: embryo stage

text: normal

text: RK1 knockout

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: ONTARIO INSTITUTE FOR CANCER RESEARCH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:ONTARIO INSTITUTE FOR CANCER RESEARCH

characteristic: age

text: ArrayExpress

text: post liver transplant

characteristic: clinical information

text: human

text: Protocols: Archived fresh-frozen liver biopsy samples were collected from the Multi-Organ Transplant (MOT) biobank. RNA was isolated from fresh-frozen tissue using the RNeasy Mini Kit (Qiagen) as per manufacturer's protocol. Libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (Roche) following the recommended protocol.

characteristic: description

text: adult

text: non-alcoholic steatohepatitis

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: ONTARIO INSTITUTE FOR CANCER RESEARCH

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: post liver transplant

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: title


text: ONTARIO INSTITUTE FOR CANCER RESEARCH

characteristic: INSDC center name

text: public

text: ArrayExpress

text: post liver transplant

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

text: ONTARIO INSTITUTE FOR CANCER RESEARCH

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

text: post liver transplant

characteristic: clinical information

text: human

characteristic: description

text: adult

text: steatosis

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: ONTARIO INSTITUTE FOR CANCER RESEARCH

text: public

characteristic: INSDC status

text: ArrayExpress

text: post liver transplant

text: human

text: adult

text: non-alcoholic steatohepatitis

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens

text: female

characteristic: sex


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

tag: Namespace:Wellcome Sanger Institute

characteristic: Submitter Id

text: 16 gestational week

unit: week

text: ArrayExpress

characteristic: broker name

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: Protocols: All tissue samples used for this study were obtained with written informed consent from all participants in accordance with the guidelines in The Declaration of Helsinki 2000. Human embryo and fetal samples were obtained from the MRC and Wellcome-funded Human Developmental Biology Resource (HDBR43, http:// www.hdbr.org), with appropriate maternal written consent and approval from the Newcastle and North Tyneside NHS Health Authority Joint Ethics Committee (08/H0906/21+5). The HDBR is regulated by the UK Human Tissue Authority (HTA; www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice. We used the previous protocol optimised for gonadal dissociation and available at protocols.io. https://www.protocols.io/view/human-embryonic-gonad-dissociation-with-trypsin-ed-66fhhbn In short, tissues were cut into <1 mm segments before being transferred to a 15ml tube. Tissues were digested with Trypsin/EDTA 0.25% between 5-15 minutes at 37°C with intermittent shaking. Digested tissue was passed through a 100 µm filter, and cells collected by centrifugation (500 x g for 5 minutes at 4°C). Cells were washed with flow buffer (PBS containing 5 % (v/v) FBS and 2 mM EDTA) prior to cell counting. library For the scRNA-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell 5′ Kit v.1.0, v1.1 and v.2 (10X Genomics) to attain between 2,000 and 10,000 cells per reaction. Library preparation was carried out according to the manufacturer’s protocol. Library preparation was carried out according to the manufacturer’s protocol for the Chromium Single Cell 5′ Kit v.1.0, v1.1 and v.2 (10X Genomics).

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 8.8 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Wellcome Sanger Institute

text: public

characteristic: INSDC status

text: 17 gestational week

characteristic: age

text: ArrayExpress

text: gonad

text: human

characteristic: description

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female


text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 9 gestational week

text: ArrayExpress

characteristic: broker name

text: gonad

text: human

text: embryo

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 7.5 gestational week

text: ArrayExpress

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 11 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: female


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 11 gestational week

characteristic: age

text: ArrayExpress

text: gonad

text: human

characteristic: description

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: 8.4 gestational week

text: ArrayExpress

characteristic: broker name

text: gonad

text: human

text: embryo

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

text: 6 gestational week

text: ArrayExpress

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

characteristic: title


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

text: 14 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 19 gestational week

characteristic: age

text: ArrayExpress

text: gonad

text: human

characteristic: description

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

characteristic: Submitter Id

text: 9 gestational week

text: ArrayExpress

characteristic: broker name

text: gonad

text: human

text: embryo

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

characteristic: sex


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: 8.6 gestational week

text: ArrayExpress

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

text: public

text: 12 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 7 gestational week

characteristic: age

text: ArrayExpress

text: gonad

text: human

characteristic: description

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: 16 gestational week

text: ArrayExpress

characteristic: broker name

text: gonad

text: human

text: embryo

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 17 gestational week

text: ArrayExpress

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

text: public

text: 14 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: male


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 12 gestational week

characteristic: age

text: ArrayExpress

text: gonad

text: human

characteristic: description

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

text: Wellcome Sanger Institute

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: 15 gestational week

text: ArrayExpress

characteristic: broker name

text: gonad

text: human

text: embryo

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Sanger Institute

characteristic: INSDC last update

text: public

text: 16 gestational week

text: ArrayExpress

text: gonad

characteristic: cell type

text: human

characteristic: common name

text: embryo

text: normal

text: Homo sapiens

text: Homo sapiens

text: gonad

text: Homo sapiens

text: female

characteristic: title


characteristic: External Id

text: Wellcome Sanger Institute

characteristic: INSDC center name

text: public

text: 6 gestational week

text: ArrayExpress

text: gonad

text: human

text: embryo

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: gonad

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-LAST-UPDATE

text: LNU

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

text: aerobic

characteristic: oxygenation status of sample

text: 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC)

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-06-18, filter fraction:3-0.2

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

characteristic: organism

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-06-21, filter fraction:3-0.2

characteristic: title

text: water


text: LNU

text: public

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2012-06-28, filter fraction:3-0.2

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-07-02, filter fraction:3-0.2

text: water

characteristic: water environmental package


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-07-05, filter fraction:3-0.2

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2012-07-09, filter fraction:3-0.2

text: water


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-07-12, filter fraction:3-0.2

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-07-17, filter fraction:3-0.2

characteristic: title

text: water


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2012-07-24, filter fraction:3-0.2

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-07-26, filter fraction:3-0.2

text: water

characteristic: water environmental package


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-07-30, filter fraction:3-0.2

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2012-08-02, filter fraction:3-0.2

text: water


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-08-06, filter fraction:3-0.2

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

characteristic: oxygenation status of sample

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-08-09, filter fraction:3-0.2

characteristic: title

text: water


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 3-0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2012-08-13, filter fraction:3-0.2

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 3-0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

text: LMO 16S 2012-08-16, filter fraction:3-0.2

text: water

characteristic: water environmental package


text: LNU

text: public

characteristic: collection date

text: marine pelagic biome (ENVO:01000023)

characteristic: environment (biome)

text: brackish water body (ENVO:01001321)

characteristic: environment (feature)

text: brackish water (ENVO:00002019)

characteristic: environment (material)

text: Baltic Sea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: aquatic metagenome

characteristic: organism

text: aerobic

characteristic: oxygenation status of sample

text: 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC)

characteristic: pcr primers

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

text: Filter setup: 0.2

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

text: 16S rRNA

text: LMO 16S 2012-09-06, filter fraction:0.2

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: LNU

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: marine pelagic biome (ENVO:01000023)

text: brackish water body (ENVO:01001321)

text: brackish water (ENVO:00002019)

text: Baltic Sea

text: mimarks-survey

text: aquatic metagenome

text: aerobic

text: LMO 16S 2011,2012,2013 (0.2, 3-0.2, 3)

characteristic: project name

text: Filter setup: 0.2

characteristic: sample collection device or method

text: aquatic metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16S rRNA

text: LMO 16S 2013-07-12, filter fraction:0.2

text: water