characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: tundra

text: upland soil

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

text: 5 cm

text: 700 m

text: metagenome

text: soil metagenome

characteristic: organism

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o427


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: tundra

characteristic: environment (biome)

text: upland soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: 5 cm

unit: m

characteristic: geographic location (depth)

text: 700 m

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: DARKFUNCTIONS

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: tundra soil metagenome o481

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: tundra

text: upland soil

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

text: 5 cm

text: 700 m

text: metagenome

text: soil metagenome

characteristic: organism

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o517


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: tundra

characteristic: environment (biome)

text: upland soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: 5 cm

characteristic: geographic location (depth)

text: 700 m

characteristic: geographic location (elevation)

text: metagenome

text: soil metagenome

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o577


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

text: public

text: tundra

text: upland soil

text: soil

text: Finland

text: 5 cm

text: 700 m

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: DARKFUNCTIONS

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: tundra soil metagenome o667

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: tundra

text: upland soil

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

text: 5 cm

text: 700 m

text: metagenome

text: soil metagenome

characteristic: organism

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o811


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: tundra

characteristic: environment (biome)

text: upland soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: 5 cm

characteristic: geographic location (depth)

text: 700 m

characteristic: geographic location (elevation)

text: metagenome

text: soil metagenome

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o823


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

text: public

text: tundra

text: upland soil

text: soil

text: Finland

text: 5 cm

text: 700 m

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: DARKFUNCTIONS

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: tundra soil metagenome o835

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: tundra

text: upland soil

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

text: 5 cm

text: 700 m

text: metagenome

text: soil metagenome

characteristic: organism

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o961


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: tundra

characteristic: environment (biome)

text: upland soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: 5 cm

characteristic: geographic location (depth)

text: 700 m

characteristic: geographic location (elevation)

text: metagenome

text: soil metagenome

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

text: tundra soil metagenome o967


characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

text: public

text: tundra

text: upland soil

text: soil

text: Finland

text: 5 cm

text: 700 m

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: DARKFUNCTIONS

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: tundra soil metagenome o97

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Irish Mycobacteria Reference Laboratory (IMRL)

characteristic: Submitter Id

characteristic: collection date

text: PZA-S

characteristic: description

text: Ireland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: human

characteristic: host scientific name

text: M. tuberculosis

characteristic: isolate

text: clinical isolate

characteristic: isolation source

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis


Fen pooled sample

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Fen pooled sample

text: tundra

characteristic: environment (biome)

text: fen

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: 5 cm

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: DARKFUNCTIONS

characteristic: project name

characteristic: sample composed of

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

text: This sample represents a pool of samples used for a co-assembly - <ERS5469703,ERS5469704,ERS5469705,ERS5469706,ERS5469707,ERS5469708,ERS5469710,ERS5469709,ERS5469711,ERS5469712,ERS5469713,ERS5469714,ERS5469715,ERS5469716,ERS5469717,ERS5469718,ERS5469676,ERS5469677,ERS5469678,ERS5469679,ERS5469680,ERS5469681>


Upland pooled sample

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UH

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Upland pooled sample

characteristic: Submitter Id

characteristic: collection date

text: tundra

text: upland soil

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

text: 5 cm

text: metagenome

text: soil metagenome

text: DARKFUNCTIONS

text: soil metagenome

text: Illumina

text: soil

characteristic: soil environmental package

text: This sample represents a pool of samples used for a co-assembly - <ERS5469668,ERS5469669,ERS5469670,ERS5469671,ERS5469672,ERS5469673,ERS5469674,ERS5469675,ERS5469682,ERS5469683,ERS5469684,ERS5469685,ERS5469686,ERS5469687,ERS5469688,ERS5469689,ERS5469690,ERS5469691,ERS5469692,ERS5469693,ERS5469694,ERS5469695,ERS5469696,ERS5469697,ERS5469698,ERS5469699,ERS5469700,ERS5469701,ERS5469702,ERS5469719,ERS5469720,ERS5469721,ERS5469722,ERS5469723,ERS5469724,ERS5469725,ERS5469726,ERS5469727,ERS5469728,ERS5469729,ERS5469730,ERS5469731,ERS5469732,ERS5469733,ERS5469734,ERS5469735,ERS5469736>

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: THE JACKSON LABORATORY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:THE JACKSON LABORATORY

text: human

characteristic: common name

characteristic: description

text: Sequel

characteristic: instrument

text: Homo sapiens

characteristic: organism

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:University of Copenhagen, Faculty of Health and Medical Sciences

text: USA

text: not collected

characteristic: host health state

text: water

characteristic: host scientific name

characteristic: isolate

text: Water

text: Vibrio cholerae

text: Vibrio cholerae

text: VIBRIO CHOLERAE O75


text: University of Copenhagen, Faculty of Health and Medical Sciences

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: USA

text: not applicable

text: water

text: Water

text: Vibrio cholerae

text: Vibrio cholerae

text: VIBRIO CHOLERAE O75


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: USA

characteristic: geographic location (country and/or sea)

text: diseased

text: homo sapiens

text: human stool

text: Vibrio cholerae

text: Vibrio cholerae

text: VIBRIO CHOLERAE O141

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Spain

text: diseased

text: homo sapiens

text: human stool

text: Vibrio cholerae

text: Vibrio cholerae

text: VIBRIO CHOLERAE O141


text: public

text: India

text: diseased

text: homo saliens

text: human stool

text: Vibrio cholerae

characteristic: organism

text: Vibrio cholerae

text: VIBRIO CHOLERAE O141


characteristic: ENA-FIRST-PUBLIC

text: public

text: Taiwan

text: diseased

text: homo sapiens

text: human stool

text: Vibrio cholerae

text: Vibrio cholerae

text: VIBRIO CHOLERAE O141


characteristic: ENA-LAST-UPDATE

text: Institute for research in Biomedecine

text: public

tag: Namespace:Institute for research in Biomedecine

text: iPSC

characteristic: cell type

text: human

characteristic: common name

text: Capture HiC in a 2Mb region of chromosome XII in human human induced PSC

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Capture HiC in human induced PSC-1


text: Institute for research in Biomedecine

characteristic: INSDC last update

text: public

text: iPSC

text: human

text: Capture Hi.C in a 2Mb region of chromosome XII in human induced PSC

text: Homo sapiens

text: Homo sapiens

text: Capture HiC in human induced PSC_2


text: Institute for research in Biomedecine

text: public

characteristic: INSDC status

text: iPSC

text: human

text: Capture HiC in a 2Mb region of chromosome XII in human induced PSC

text: Homo sapiens

text: Homo sapiens

text: Capture HiC in human induced PSC_2


text: public

characteristic: SRA accession

tag: Namespace:Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: Wales

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: receipt date

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


text: Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

text: public

characteristic: SRA accession

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:University of Birmingham

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


text: Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit

text: public

characteristic: SRA accession

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: collection date

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: not provided

text: not provided


text: public

characteristic: SRA accession

tag: Namespace:School of Life Sciences, Fudan University

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: soybean

characteristic: common name

characteristic: cultivar

text: Protocols: The two-weeks-old soybean leaves were harvested for genomic resequencing Soybean plants were grown in the field at Shanghai, China DNA extraction was conducted by using Cetyltrimethyl ammonium bromide (CTAB) method Illumina-DNA-PE-library kit

characteristic: description

text: seedling

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: field study

characteristic: growth condition

text: Glycine max

text: Glycine max

text: leaf

characteristic: organism part

text: Glycine max


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: School of Life Sciences, Fudan University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: soybean

text: seedling

text: wild type genotype

text: field study

text: Glycine max

characteristic: organism

text: Glycine max

text: leaf

text: Glycine max

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: soybean

text: Protocols: The soybean meiosis cells were used for RNA extraction. Samples were harvested in the moring between 9:00 to 12:00, and about 200 cells per sample Soybean plants were grown in the field at Shanghai, China Total RNA was extracted from the meiotic cell of soybean using TRIzol® Reagent according the manufacturer instructions (Invitrogen) Illumina-RNA-library kit

text: wild type genotype

text: field study

text: Glycine max

text: Glycine max

text: sporocyte

text: Glycine max


text: Necator americanus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Necator americanus

characteristic: common name

text: Necator americanus

text: Necator americanus


text: UTRECHT UNIVERSITY

text: public

tag: Namespace:UTRECHT UNIVERSITY

text: Illumina sequencing of 18S03797-1

characteristic: description

text: USA

characteristic: geographic location (country and/or sea)

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03797-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03809-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03809-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 2160129049

text: Netherlands

text: horse

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 2160129049


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 216020506901

text: Netherlands

text: horse

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 216020506901


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S03585-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S03585-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S03684-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S03684-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S03687

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S03687


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S03881-1

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S03881-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S03930-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S03930-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04041-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04041-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04042-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04042-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04046-1

text: Netherlands

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04046-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04148-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04148-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04149-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04149-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04224-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04224-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04240-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04240-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 15S04243-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 15S04243-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 16S00675-10

text: Netherlands

text: horse

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 16S00675-10


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 16S05798

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 16S05798


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 16S06273-3

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 16S06273-3


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 16S06982-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 16S06982-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01135-1

text: Brazil

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01135-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01138-1

text: Brazil

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01138-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01140-1

text: Brazil

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01140-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01141-1

text: Brazil

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01141-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01144-1

text: Brazil

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01144-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01290-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01290-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01351-1

text: Netherlands

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01351-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S01353-1

text: Netherlands

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S01353-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S02777-1

text: Netherlands

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S02777-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S03759-1

text: Germany

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S03759-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S03760-1

text: Germany

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S03760-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S03761-1

text: Germany

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S03761-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 17S03762-1

text: Germany

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 17S03762-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02844-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02844-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02845-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02845-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02847-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02847-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02848-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02848-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02849-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02849-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02851

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02851


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02853-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02853-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02857-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02857-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02858

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02858


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02859-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02859-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02860

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02860


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02862

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02862


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02863

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02863


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S02865

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S02865


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03233-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03233-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03235-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03235-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03236-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03236-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03238

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03238


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03241

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03241


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03242-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03242-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03799-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03799-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03801-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03801-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03802-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03802-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03803-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03803-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03804-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03804-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03806-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03806-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03808-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03808-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03810-1

text: USA

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03810-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03813-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03813-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03814-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03814-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03820-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03820-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03822-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03822-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03823-1

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03823-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03824-1

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03824-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03827-1

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03827-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03828-1

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03828-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03829-1

text: USA

text: ovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03829-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03831-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03831-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03833-2

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03833-2


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03834-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03834-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03836-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03836-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03837-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03837-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03838-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03838-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03839-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03839-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03840-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03840-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03841-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03841-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03851-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03851-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S03852-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S03852-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S04139-1

text: Belgium

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S04139-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 18S04140-1

text: Belgium

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 18S04140-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 19S00791-1

text: India

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 19S00791-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 19S00906-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 19S00906-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 19S00907-1

text: USA

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 19S00907-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 19S01514-1

text: Belgium

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 19S01514-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 78-1

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 78-1


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 80-109

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 80-109


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 81-173

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 81-173


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 83-88

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 83-88


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 84-91

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 84-91


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 87-76

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 87-76


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of 89-2

text: USA

text: human

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of 89-2


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of ru-2008-42580-5

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of ru-2008-42580-5


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of ru-2009-08871-5

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of ru-2009-08871-5


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of ru-2009-11615-002

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of RU201508797022

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of RU201508797022


text: UTRECHT UNIVERSITY

text: public

text: Illumina sequencing of RU201508797030

text: Netherlands

text: bovine

text: Campylobacter fetus

text: Campylobacter fetus

text: Illumina sequencing of RU201508797030


text: Salmonella enterica

text: SC

text: public

text: Salmonella enterica

text: Salmonella enterica

text: Salmonella enterica


text: Escherichia coli

text: SC

text: public

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli


text: Escherichia Coli

text: SC

text: public

text: Escherichia Coli

text: Escherichia coli

text: Escherichia coli


text: MIGS.eu

characteristic: BioSampleModel

text: ELIXIR Workflow Implementation Study

text: public

tag: Namespace:ELIXIR Workflow Implementation Study

text: no

characteristic: axenic

text: William Wilson

text: NCBI

characteristic: broker name

text: no

characteristic: clonal

text: 0 umol/L

text: 12 hr

text: f/2 amended seawater

text: 400 (umol photons/m^2/s)

text: f/2 for all nutrients, no Na2SiO3 added

text: 12 hr

text: 882 umol/L

text: 36.2 umol/L

text: 34 psu

text: 0 umol/L

text: 25 C

text: 12794 nmol/L

text: Pacific Ocean

text: Pacific Ocean

text: 41.27 S 174.795 E

text: uncultured Symbiodinium

text: William

text: Wilson

text: Marine microbial eukaryote transcriptome sequencing project

text: uncultured Symbiodinium

characteristic: strain


text: MIGS.eu

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ELIXIR Workflow Implementation Study

characteristic: INSDC center name

text: public

text: no

text: Jan Pawlowski

text: NCBI

text: yes

text: 4 umol/L

text: 10 hr

text: 1/8 Erdschreiber media plus filtered sea water

text: 247 (umol photons/m^2/s)

text: 14 hr

text: 12 umol/L

text: 2 umol/L

text: Chlorella sp., bacteria, diatoms

text: 35 psu

text: 1 umol/L

text: 22 C

text: 15 nmol/L

text: Foraminifera environmental sample

text: Jan

text: Pawlowski

text: Foraminifera environmental sample


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: no

text: Jan Pawlowski

text: NCBI

text: no

text: nonculturable

text: 653 (umol photons/m^2/s)

text: nonculturable

text: algae and bacteria

text: 40 psu

text: Foraminifera environmental sample

characteristic: organism

text: Jan

text: Pawlowski

text: Foraminifera environmental sample


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: no

text: Patrick Keeling

text: NCBI

text: yes

text: Aquatic: marine

text: 0 umol/L

text: 12 hr

text: f/2 seawater

text: 404 (umol photons/m^2/s)

text: 12 hr

text: 882 umol/L

text: 36.2 umol/L

text: 21 psu

text: 0 umol/L

text: 22 C

text: 23000 nmol/L

text: uncultured alveolate

text: Patrick

text: Keeling

text: uncultured alveolate


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

text: public

text: no

text: Willie Wilson

text: NCBI

text: 100 umol/L

text: 13 hr

text: 753 (umol photons/m^2/s)

text: added 200 ul of 500 mol NH4Cl per liter

text: 11 hr

text: 882 umol/L

text: 36.2 umol/L

text: 32 psu

text: 106 umol/L

text: 24 C

text: 12871.2 nmol/L

text: Mediterranean Sea

text: Mediterranean Sea

text: 42.572 N 8.73 E

text: uncultured Pelagophyceae

text: Willie

text: Wilson

text: uncultured Pelagophyceae


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

text: public

text: no

text: Jan Pawlowski

text: NCBI

text: no

text: Aquatic: marine

text: 4 umol/L

text: 10 hr

text: nonculturable

text: 440 (umol photons/m^2/s)

text: 14 hr

text: 12 umol/L

text: 2 umol/L

text: Bacteria and algae

text: 30 psu

text: 1 umol/L

text: 20 C

text: 15 nmol/L

text: North Sea

text: North Sea

text: Foraminifera environmental sample

text: Jan

text: Pawlowski

text: Foraminifera environmental sample


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

text: public

text: Mike Preston

text: NCBI

text: yes

text: 10 m

characteristic: depth

text: 30 psu

text: 20 C

text: Aquatic: marine

text: 0 umol/L

text: 390 ppm

text: 13 hr

text: 0 ml/L

text: L1 amended seawater

text: 488 (umol photons/m^2/s)

text: 11 hr

text: 882 umol/L

text: 36.2 umol/L

text: 33 psu

text: 106 umol/L

text: 20 C

text: 11.7 nmol/L

text: 12871.2 nmol/L

text: 0 umol/L

text: Pacific Ocean

text: Pacific Ocean

text: 31.35 S 78.1 W

text: uncultured marine Prymnesiales

text: Mike

text: Preston

text: uncultured marine Prymnesiales


text: MIGS.eu

characteristic: BioSampleModel

text: ELIXIR Workflow Implementation Study

text: public

text: no

text: Willie Wilson

text: NCBI

text: no

text: 0 m

text: Aquatic: marine

text: 12 hr

text: 490 (umol photons/m^2/s)

text: 12 hr

text: 35 psu

text: 4 C

text: Arctic Ocean

text: Arctic Ocean

text: 70.5 N 135.5 W

text: uncultured eukaryote

text: Willie

text: Wilson

text: uncultured eukaryote


text: MIGS.eu

text: ELIXIR Workflow Implementation Study

text: public

text: no

characteristic: axenic

text: Mike Preston

text: NCBI

text: yes

text: 10 m

text: 30 psu

text: 20 C

text: Aquatic: marine

text: 0 umol/L

text: 390 ppm

text: 13 hr

text: 0 ml/L

text: L1 amended seawater

text: 489 (umol photons/m^2/s)

text: 11 hr

text: 882 umol/L

text: 36.2 umol/L

text: 33 psu

text: 106 umol/L

text: 20 C

text: 11.7 nmol/L

text: 12871.2 nmol/L

text: 0 umol/L

text: Pacific Ocean

text: Tidepool

text: Pacific Ocean

text: 21.102 N 157.06 W

text: uncultured eukaryote

text: Mike

text: Preston

text: uncultured eukaryote


text: MIGS.eu

text: public

tag: Namespace:Roscoff Biological Station - Sorbonne University

text: yes

text: Connie Lovejoy

text: NCBI

characteristic: broker name

text: no

text: 0 m

text: Aquatic: marine

text: 0 umol/L

text: 24 hr

text: K amended sea water

text: 196 (umol photons/m^2/s)

text: antibiotics final concentrations: penicilin: 25mg/L; neomycin 20mg/L; kanamycin 20mg/L; no NH4Cl added

text: 0 hr

text: 882 umol/L

text: 10 umol/L

text: 33 psu

text: 504 umol/L

text: 6 C

text: 11700 nmol/L

text: 123766 nmol/L

text: 0 umol/L

text: Greenland: Baffin Bay

text: Greenland: Baffin Bay

text: 77.0014 N 77.2383 W

text: uncultured eukaryote

text: Connie

text: Lovejoy

text: uncultured eukaryote


text: THE CHINESE UNIVERSITY OF HONG KONG

text: public

tag: Namespace:THE CHINESE UNIVERSITY OF HONG KONG

text: The Chinese University of Hong Kong

text: Epstein-Barr virus

characteristic: common name

characteristic: description

text: Hong Kong

characteristic: geographic location (country and/or sea)

text: Human gammaherpesvirus 4

text: Human gammaherpesvirus 4

text: primary NPC tumor

characteristic: title


characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Salmonella enterica subsp. enterica serovar Typhi str. CT18


text: Salmonella enterica subsp. enterica serovar Typhi

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public


text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Salmonella enterica subsp. enterica serovar Paratyphi A

characteristic: organism


text: Salmonella enterica subsp. enterica serovar Paratyphi

text: SC

text: public


text: public

tag: Namespace:Key Laboratory of Environmental and Applied Microbiology, CAS

characteristic: collection date

text: water

characteristic: environment (biome)

text: water

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

characteristic: investigation type

text: gut metagenome

characteristic: project name

text: gut metagenome

text: MiSeq

characteristic: sequencing method

text: Corpse


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Key Laboratory of Environmental and Applied Microbiology, CAS

characteristic: INSDC center name

text: public

text: water

text: water

text: water

text: China

characteristic: geographic location (country and/or sea)

text: host-associated

text: mimarks-survey

text: gut metagenome

text: gut metagenome

text: MiSeq

text: Corpse

characteristic: title


text: Leishmania aethiopica

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leishmania aethiopica

characteristic: common name

text: Leishmania aethiopica

characteristic: organism

text: Leishmania aethiopica


text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


text: IRSICAIXA

text: public

text: feces metagenome

text: feces metagenome


text: Plasmodium falciparum 3D97

characteristic: ArrayExpress-SPECIES

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D97

characteristic: common name

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D98

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D98

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D99

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D99

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D100

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D100

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D101

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D101

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D102

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D102

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D103

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D103

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D105

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D105

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D104

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D104

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D106

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D106

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D108

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D108

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D107

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D107

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D109

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D109

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D110

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D110

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D111

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D111

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D112

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D112

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D113

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D113

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D114

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D114

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D115

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D115

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D116

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D116

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D117

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D117

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D118

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D118

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D119

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D119

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D120

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D120

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D121

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D121

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D122

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D122

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D123

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D123

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D124

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D124

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D125

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D125

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D126

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D126

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D127

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D127

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D128

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D128

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D130

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D130

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D129

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D129

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D132

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D132

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D131

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D131

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D133

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D133

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D134

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D134

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D135

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D135

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D136

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D136

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D138

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D138

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D137

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D137

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D140

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D140

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D139

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D139

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D141

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D141

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D142

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D142

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D144

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D144

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D145

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D145

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D143

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D143

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D146

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D146

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D147

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D147

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D160

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D160

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D161

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D161

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D163

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D163

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D162

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D162

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D164

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D164

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D165

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D165

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D166

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D166

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D167

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D167

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D168

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D168

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D169

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D169

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D170

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D170

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D171

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D171

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D172

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D172

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D149

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D149

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D148

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D148

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D150

text: Plasmodium fal. NF54

text: SC

text: public

text: Plasmodium falciparum 3D150

text: Plasmodium falciparum 3D7

text: Plasmodium falciparum 3D7


text: Plasmodium falciparum 3D152

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D152

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D151

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D151

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D154

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D154

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D153

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D153

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D155

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D155

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D156

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D156

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D157

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D157

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D158

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D158

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


text: Plasmodium falciparum 3D159

text: Plasmodium_knowlesi (strainH)

text: SC

text: public

text: Plasmodium falciparum 3D159

text: Plasmodium knowlesi strain H

text: Plasmodium knowlesi strain H


E-MTAB-10081:A scRNA-seq

text: TONGJI UNIVERSITY

text: public

text: E-MTAB-10081:A scRNA-seq

tag: Namespace:TONGJI UNIVERSITY

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B-lymphoblast

text: house mouse

text: Protocols: Neonatal sample collection: 1.The neonatal mice were sacrificed by cervical dislocation. 2.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. 3.Then massage the peritoneum gently and insert a 26g needle attached to the 1 ml syringe into the peritoneum to collect the fluid. 4.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. Then make an incision in the skin of the abdomen, and collect the fluid with pipette. 5.Then adding 200μl DPBS with 2% FBS to the peritoneal cavity and aspirating the fluid. Repeating this steps for three times to collect more peritoneal cells. 6.Spin the collected cell suspension at 400 x g for 8 minutes, 7.Red blood cells were removed using the ACK Lysing Buffer 8.Centrifuge the lavage fluid of the peritoneal cells at 400 x g for 6 minutes and resuspending the cell pellet in 90 μl MACS Buffer which is prepared by dissolving bovine serum protein with auto MACS Rinsing Solution to form a 0.5% BSA solution. 9.Then the CD19 MicroBeads were added to the suspension, and the mixture was incubated at 4℃ for 10 minutes. 10.Applying the cell suspension onto the MS column placed in the magnetic field of a MACS Separator. And the labeled CD19 positive cells were retained on the column. 11.Collecting the positive selected cells for further research by removing the column from the magnetic field and flushing the column with MACS buffer. 12.The collected CD19 positive cells were filtered with 30-μm Cell Strainer and then were counted with the cell auto counter. 13.Centrifuging the cells and resuspending the cells with suitable volume buffer. 3' v3 Nucleic extraction (3ʹ v3.1)1. GEMs were generated by combining barcoded Single Cell 3ʹ v3.1 Gel Beads, a Master Mix containing cells, and Partitioning Oil onto Chromium Next GEM Chip G. To achieve single cell resolution, cells were delivered at a limiting dilution, such that the majority (~90-99%) of generated GEMs contained no cell, while the remainder largely contained a single cell. 2. Immediately following GEM generation, the Gel Bead was dissolved, primers were released, and any co-partitioned cell was lysed. Primer containing: an Illumina TruSeq Read 1 (read 1 sequencing primer), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), 30 nt poly (dT) sequence. Primers were mixed with the lysate and a Master Mix containing reverse transcription (RT) reagents. Incubation of the GEMs produces barcoded, full-length cDNA from poly-adenylated mRNA.3. After incubation, GEMs were broken and pooled fractions are recovered. Silane magnetic beads were used to purify the first-strand cDNA from the post GEM-RT reaction mixture, which included leftover biochemical reagents and primers. Barcoded, full-length cDNA was amplified via PCR to generate sufficient mass for library construction. Firstly, Perform the cDNA amplification, and clean up the products. Fragmentation, End Repair & A-tailing 1. Vortex Fragmentation Buffer. Verify there is no precipitate. Prepare Fragmentation Mix (Fragmentation Buffer, Fragmentation Enzyme) on ice. Pipette mix and centrifuge briefly. 2.Transfer only 10 μl purified cDNA sample from cDNA Cleanup to a tube strip. Add 25 μl Buffer EB to each sample. And add 15 μl Fragmentation Mix to each sample. Pipette mix 15x on ice. Centrifuge briefly. 3. Transfer into the pre-cooled thermal cycler (4°C) and press “SKIP” to initiate the protocol. Post Fragmentation, End Repair & A-tailing Double Sided Size Selection - SPRIselect 4. Vortex to resuspend SPRIselect reagent. Add 30 μl SPRIselect (0.6X) reagent to each sample. Pipette mix 15x. Incubate 5 min at room temperature. 5. Place on the magnet High until the solution clears. 6. Remove 80 μl supernatant 7. Add 125 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 8. Centrifuge briefly. Place on the magnet•Low until the solution clears. Remove remaining ethanol. 9. Remove from the magnet. Add 50.5 μl Buffer EB to each sample. Pipette mix 15x. 10. Incubate 2 min at room temperature. 11. Place on the magnet•High until the solution clears. 12. Transfer 50 μl sample to a new tube strip. Adaptor Ligation 13. Prepare Adaptor Ligation Mix (Ligation Buffer, DNA ligase, Adaptor Oligos) 14. Add 50 μl Adaptor Ligation Mix to 50 μl sample. Pipette mix 15x (pipette set to 90 μl). Centrifuge briefly. 15. Incubate at 20 °C for 15 min in a thermal cycler. Post Ligation Cleanup -SPRIselect 16. Vortex to resuspend SPRIselect Reagent. Add 80 μl SPRIselect Reagent (0.8X) to each sample. Pipette mix 15x (pipette set to 150 μl). 17. Incubate 5 min at room temperature. Place on the magnet•High until the solution clears. Remove the supernatant. 18. Add 200 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 19. Centrifuge briefly. Place on the magnet•Low. 20. Remove any remaining ethanol. Air dry for 2 min. 21. Remove from the magnet. Add 30.5 μl Buffer EB. Pipette mix 15x. 22. Incubate 2 min at room temperature. 23. Place on the magnet•Low until the solution clears. 24. Transfer 30 μl sample to a new tube strip. Sample Index PCR 25. Prepare Sample Index PCR Mix. Add 60 μl Sample Index PCR Mix to 30 μl sample. 26. Add 10 μl of an individual Single Index to each well and record the well ID used. Pipette mix 5x. Centrifuge briefly. 27. Incubate in a thermal cycler with the following protocol. The protocol is : 98 °C for 45 s, several cycles of 98 °C for 20 s, 54 °C for 30 s, and 72 °C for 20s, 72 °C for 1 min, and 4 °C holding. Post Sample Index PCR Double Sided Size Selection - SPRIselect 28. Vortex to resuspend the SPRIselect reagent. Add 60 μl SPRIselect Reagent (0.6X) to each sample. Pipette mix 15x. Incubate 5 min at room temperature. 29. Place the magnet•High until the solution clears. 30. Transfer 150 μl supernatant to a new tube strip. 31. Vortex to resuspend the SPRIselect reagent. Add 20 μl SPRIselect Reagent (0.8X) to each sample. Pipette mix 15x (pipette set to 150 μl). 32. Incubate 5 min at room temperature. 33. Place the magnet•High until the solution clears. 34. Remove 165 μl supernatant. 35. With the tube still in the magnet, add 200 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 36. Centrifuge briefly. Place on the magnet•Low. Remove remaining ethanol. m. Remove from the magnet. Add 35.5 μl Buffer EB. Pipette mix 15x. 37. Incubate 2 min at room temperature. 38. Place on the magnet•Low until the solution clears. 39. Transfer 35 μl to a new tube strip. 40. Perform the quality control of the cDNA library.

characteristic: description

text: neonatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool of 10

characteristic: individual

text: Mus musculus

text: Mus musculus

text: peritoneal cavity fluid

characteristic: organism part

text: Mus musculus

text: male and female

characteristic: sex

characteristic: strain

text: A scRNA-seq


E-MTAB-10081:B scRNA-seq

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TONGJI UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10081:B scRNA-seq

text: ArrayExpress

text: B-lymphoblast

text: house mouse

text: Protocols: Adult and elderly sample collection: 1. The mice were sacrificed by cervical dislocation, spraying it with 70% ethanol. 2. Use a scissors and forceps to cut the outer skin of the peritoneum and gently pull it back to expose the inner skin lining the peritoneal cavity. 3. Inject 10 ml of ice cold PBS (with 2% FCS) into the peritoneal cavity with a 27g needle. Push the needle slowly in the peritoneum. 4. After injection, gently massage the peritoneum to dislodge any attached cells into the PBS solution. 5. Insert a 25 g needle, bevel up, attached to a 10 ml syringe in the peritoneum and collect the fluid while moving the tip of the needle gently to avoid clogging by the fat tissue or other organs. Collect as much fluid as possible and depositing the collected cell suspension in tubes kept on ice after removing the needle from the syringe. 6. Repeat step 3-5. 7. Make an incision in the inner skin of the peritoneum and use a pipette to aspirate the fluid. 8. Spin the collected cell suspension at 400 x g for 8 minutes, 9. Red blood cells were removed using the ACK Lysing Buffer 10. Centrifuge the lavage fluid of the peritoneal cells at 400 x g for 6 minutes and resuspending the cell pellet in 90 μl MACS Buffer which is prepared by dissolving bovine serum protein with auto MACS Rinsing Solution to form a 0.5% BSA solution. 11. Then the CD19 MicroBeads were added to the suspention, and the mixture was incubated at 4℃ for 10 minutes. 12. Applying the cell suspention onto the MS column placed in the magnetic field of a MACS Separator. And the labeled CD19 positive cells were retained on the column. 13. Collecting the positive selected cells for further research by removing the column from the magnetic field and flushing the column with MACS buffer. 14. The collected CD19 positive cells were filterd with 30-μm Cell Strainer and then were counted with the cell auto counter. 15. Centrifuging the cells and resuspending the cells with suitable volume buffer to ensure that the concentration is 3 x 105 - 1 x 106 cells/ml. 3' v3 Nucleic extraction (3ʹ v3.1)1. GEMs were generated by combining barcoded Single Cell 3ʹ v3.1 Gel Beads, a Master Mix containing cells, and Partitioning Oil onto Chromium Next GEM Chip G. To achieve single cell resolution, cells were delivered at a limiting dilution, such that the majority (~90-99%) of generated GEMs contained no cell, while the remainder largely contained a single cell. 2. Immediately following GEM generation, the Gel Bead was dissolved, primers were released, and any co-partitioned cell was lysed. Primer containing: an Illumina TruSeq Read 1 (read 1 sequencing primer), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI), 30 nt poly (dT) sequence. Primers were mixed with the lysate and a Master Mix containing reverse transcription (RT) reagents. Incubation of the GEMs produces barcoded, full-length cDNA from poly-adenylated mRNA.3. After incubation, GEMs were broken and pooled fractions are recovered. Silane magnetic beads were used to purify the first-strand cDNA from the post GEM-RT reaction mixture, which included leftover biochemical reagents and primers. Barcoded, full-length cDNA was amplified via PCR to generate sufficient mass for library construction. Firstly, Perform the cDNA amplification, and clean up the products. Fragmentation, End Repair & A-tailing 1. Vortex Fragmentation Buffer. Verify there is no precipitate. Prepare Fragmentation Mix (Fragmentation Buffer, Fragmentation Enzyme) on ice. Pipette mix and centrifuge briefly. 2.Transfer only 10 μl purified cDNA sample from cDNA Cleanup to a tube strip. Add 25 μl Buffer EB to each sample. And add 15 μl Fragmentation Mix to each sample. Pipette mix 15x on ice. Centrifuge briefly. 3. Transfer into the pre-cooled thermal cycler (4°C) and press “SKIP” to initiate the protocol. Post Fragmentation, End Repair & A-tailing Double Sided Size Selection - SPRIselect 4. Vortex to resuspend SPRIselect reagent. Add 30 μl SPRIselect (0.6X) reagent to each sample. Pipette mix 15x. Incubate 5 min at room temperature. 5. Place on the magnet High until the solution clears. 6. Remove 80 μl supernatant 7. Add 125 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 8. Centrifuge briefly. Place on the magnet•Low until the solution clears. Remove remaining ethanol. 9. Remove from the magnet. Add 50.5 μl Buffer EB to each sample. Pipette mix 15x. 10. Incubate 2 min at room temperature. 11. Place on the magnet•High until the solution clears. 12. Transfer 50 μl sample to a new tube strip. Adaptor Ligation 13. Prepare Adaptor Ligation Mix (Ligation Buffer, DNA ligase, Adaptor Oligos) 14. Add 50 μl Adaptor Ligation Mix to 50 μl sample. Pipette mix 15x (pipette set to 90 μl). Centrifuge briefly. 15. Incubate at 20 °C for 15 min in a thermal cycler. Post Ligation Cleanup -SPRIselect 16. Vortex to resuspend SPRIselect Reagent. Add 80 μl SPRIselect Reagent (0.8X) to each sample. Pipette mix 15x (pipette set to 150 μl). 17. Incubate 5 min at room temperature. Place on the magnet•High until the solution clears. Remove the supernatant. 18. Add 200 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 19. Centrifuge briefly. Place on the magnet•Low. 20. Remove any remaining ethanol. Air dry for 2 min. 21. Remove from the magnet. Add 30.5 μl Buffer EB. Pipette mix 15x. 22. Incubate 2 min at room temperature. 23. Place on the magnet•Low until the solution clears. 24. Transfer 30 μl sample to a new tube strip. Sample Index PCR 25. Prepare Sample Index PCR Mix. Add 60 μl Sample Index PCR Mix to 30 μl sample. 26. Add 10 μl of an individual Single Index to each well and record the well ID used. Pipette mix 5x. Centrifuge briefly. 27. Incubate in a thermal cycler with the following protocol. The protocol is : 98 °C for 45 s, several cycles of 98 °C for 20 s, 54 °C for 30 s, and 72 °C for 20s, 72 °C for 1 min, and 4 °C holding. Post Sample Index PCR Double Sided Size Selection - SPRIselect 28. Vortex to resuspend the SPRIselect reagent. Add 60 μl SPRIselect Reagent (0.6X) to each sample. Pipette mix 15x. Incubate 5 min at room temperature. 29. Place the magnet•High until the solution clears. 30. Transfer 150 μl supernatant to a new tube strip. 31. Vortex to resuspend the SPRIselect reagent. Add 20 μl SPRIselect Reagent (0.8X) to each sample. Pipette mix 15x (pipette set to 150 μl). 32. Incubate 5 min at room temperature. 33. Place the magnet•High until the solution clears. 34. Remove 165 μl supernatant. 35. With the tube still in the magnet, add 200 μl 80% ethanol to the pellet. Wait 30 sec. Remove the ethanol. Repeat this step. 36. Centrifuge briefly. Place on the magnet•Low. Remove remaining ethanol. m. Remove from the magnet. Add 35.5 μl Buffer EB. Pipette mix 15x. 37. Incubate 2 min at room temperature. 38. Place on the magnet•Low until the solution clears. 39. Transfer 35 μl to a new tube strip. 40. Perform the quality control of the cDNA library.

text: adult

text: wild type genotype

text: pool of 2

text: Mus musculus

text: Mus musculus

text: peritoneal cavity fluid

text: Mus musculus

text: male

text: B scRNA-seq


E-MTAB-10081:C scRNA-seq

text: TONGJI UNIVERSITY

text: public

characteristic: SRA accession

text: E-MTAB-10081:C scRNA-seq

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: B-lymphoblast

text: house mouse

characteristic: common name

text: adult

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: peritoneal cavity fluid

text: Mus musculus

text: female

text: C scRNA-seq

characteristic: title


E-MTAB-10081:D scBCR-seq

text: TONGJI UNIVERSITY

text: public

text: E-MTAB-10081:D scBCR-seq

text: ArrayExpress

characteristic: broker name

text: B-lymphoblast

text: house mouse

text: Protocols: Neonatal sample collection: 1.The neonatal mice were sacrificed by cervical dislocation. 2.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. 3.Then massage the peritoneum gently and insert a 26g needle attached to the 1 ml syringe into the peritoneum to collect the fluid. 4.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. Then make an incision in the skin of the abdomen, and collect the fluid with pipette. 5.Then adding 200μl DPBS with 2% FBS to the peritoneal cavity and aspirating the fluid. Repeating this steps for three times to collect more peritoneal cells. 6.Spin the collected cell suspension at 400 x g for 8 minutes, 7.Red blood cells were removed using the ACK Lysing Buffer 8.Centrifuge the lavage fluid of the peritoneal cells at 400 x g for 6 minutes and resuspending the cell pellet in 90 μl MACS Buffer which is prepared by dissolving bovine serum protein with auto MACS Rinsing Solution to form a 0.5% BSA solution. 9.Then the CD19 MicroBeads were added to the suspension, and the mixture was incubated at 4℃ for 10 minutes. 10.Applying the cell suspension onto the MS column placed in the magnetic field of a MACS Separator. And the labeled CD19 positive cells were retained on the column. 11.Collecting the positive selected cells for further research by removing the column from the magnetic field and flushing the column with MACS buffer. 12.The collected CD19 positive cells were filtered with 30-μm Cell Strainer and then were counted with the cell auto counter. 13.Centrifuging the cells and resuspending the cells with suitable volume buffer. (5’)GEM generation and reverse transcription1. GEMs are generated by combining barcoded Single Cell 5’ Gel Beads, a Master Mix with cells, and Partitioning Oil on a microfluidic chip. To achieve single cell resolution, the cells are delivered at a limiting dilution, such that the majority (~90 - 99%) of generated GEMs contains no cell, while the remainder largely contain a single cell. Immediately following generation of a GEM, the Single Cell 5’ Gel Bead is dissolved and any co-partitioned cell is lysed.2. Upon dissolution of the Single Cell 5’ Gel Bead in a GEM, oligonucleotides containing (i) an Illumina R1 sequence (read 1 sequencing primer), (ii) a 16 nt 10x Barcode, (iii) a 10 nt Unique Molecular Identifier (UMI), and (iv) a 13 nt Switch Oligo are released and mixed with cell lysate and a Master Mix that contains reverse transcription (RT) reagents and poly(dT) primers. Incubation of the GEMs then produces barcoded, full-length cDNA from poly-adenylated mRNA. After incubation, the GEMs are broken and the pooled post GEM-RT reaction mixtures are recovered.3. The pooled post GEM-RT reaction mixture contains barcoded first-strand cDNA from poly-adenylated mRNA, as well as leftover biochemical reagents and primers. Silane magnetic beads are used to purify the cDNA from this mixture. Barcoded, full-length V(D)J segments can then be directly enriched from the purified post GEM-RT product by TCR/Ig constant region-specific PCR amplification prior to library construction. Alternatively, the entire purified post GEM-RT product can be amplified by PCR and aliquoted prior to enriching for barcoded, full-length V(D)J segments. (BCR-seq)BCR region enriched library construction1. Perform the cDNA amplification, and clean up the products.2. Amplified cDNA underwent two rounds of target enrichment using nested primer pairs specific for mouse BCR constant regions.3. Clean up the BCR target enrichment 2 from amplified cDNA reaction with the SPRIselect Reagent.4. Quality control were performed on the Alilent Bioanalyser.Enriched Library construction1. Dispense the appropriate volume for 50 ng (Enrichment from cDNA) into each well of a PCR 8-tube strip on a chilled metal block resting on ice.2. Prepare a thermal cycler with the following incubation protocol and initiate the 4°C precool block step prior to assembling the Fragmentation Mix. The incubation protocol is: 4 °C holding, 32 °C for 2 min, 65 °C for 30 min, 4 °C holding.3. Vortex the Fragmentation Buffer. Prepare the Fragmentation Mix on ice. Add the reagents in the order (Nuclease-free water, Fragmentation Buffer, FragmentationEnzyme Blend). Pipette mix thoroughly and centrifuge briefly.4. Dispense 30 µl Fragmentation Mix into each tube containing 20 µl Target Enrichment 2 product. Pipette mix 15 times on ice and centrifuge briefly before returning the tube strip to a chilled metal block resting on ice.5. Transfer the chilled tube strip into the pre-cooled thermal cycler (4°C) and press “SKIP” to initiate the Fragmentation protocol.Adaptor Ligation6. Prepare Adaptor Ligation Mix by adding the reagents in the order (Nuclease-Free Water, Ligation Buffer, DNA Ligase, Adaptor Mix).7. Remove tube strip containing Fragmentation, End-Repair & A-tailing Product from the thermal cycler.8. Add 50 µl Adaptor Ligation Mix to each tube containing 50 µl sample from the Fragmentation, End Repair and A-tailing Reaction. Pipette mix 15 times and centrifuge briefly.9. Incubate in a thermal cycler at 20 °C for 15 min.Post Ligation Cleanup - SPRIselect10. Vortex the SPRIselect Reagent until fully resuspended. Add 80 μl SPRIselect Reagent (0.8X) to each sample in the tube strip and pipette mix 15 times.11. Incubate the tube strip at room temperature for 5 min.12. Place the tube strip in a 10x™ Magnetic Separator in the High position until the solution is clear. Carefully remove and discard the supernatant.13. Add 200 µl 80% ethanol to the pellet and stand for 30 sec. 14. Carefully remove and discard the ethanol wash. Repeat this step.15. Centrifuge the tube strip briefly and return it to a 10x Magnetic Separator in the low position.16. Remove and discard any remaining ethanol and allow the samples to air dry for 2 min.17. Remove the tube strip from the 10x Magnetic Separator and add 30.5 µl Buffer EB.Pipette mix 15 times.18. Incubate the tube strip at room temperature for 2 min.19. Place the tube strip in a 10x Magnetic Separator in the Low position until the solution is clear.20. Transfer 30 µl of sample to a new tube strip.Sample Index PCR21. Prepare Sample Index PCR Mix by adding the reagents in the order (Nuclease-Free Water, Amplification Master Mix, SI-PCR Primer). Pipette mix thoroughly and centrifuge briefly.22. Add 60 µl Sample Index PCR Mix to each tube containing 30 µl purified Post Ligation sample.23. Add 10 µl of an individual Chromium i7 Sample Index to each well and record theirassignment. Pipette mix 5 times (pipette set to 90 μl) and centrifuge briefly.24. Index the library DNA in a thermal cycler with the following protocol. The protocol is: 98 °C for 45 s, 9 cycles of 98 °C for 20 s, 54 °C for 30 s, and 72 °C for 20s, 72 °C for 1 min, and 4 °C holding.25. Store the tube strip at 4°C for up to 72 h or proceed directly to Post Sample Index PCR Cleanup.26. Clean up the sample index PCR product with SPRIselect Reagent.27. Perform the quality control of the cDNA library.

characteristic: description

text: neonatal

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: pool of 7

characteristic: individual

text: Mus musculus

text: Mus musculus

text: peritoneal cavity fluid

characteristic: organism part

text: Mus musculus

text: male and female

text: D scBCR-seq


E-MTAB-10081:D scRNA-seq

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TONGJI UNIVERSITY

text: public

text: E-MTAB-10081:D scRNA-seq

text: ArrayExpress

text: B-lymphoblast

text: house mouse

text: Protocols: Neonatal sample collection: 1.The neonatal mice were sacrificed by cervical dislocation. 2.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. 3.Then massage the peritoneum gently and insert a 26g needle attached to the 1 ml syringe into the peritoneum to collect the fluid. 4.Inject 200 μl ice cold PBS (with 2% FCS) using a 1 ml syringe with 26g needle into the abdominal cavity of the neonatal mice. Then make an incision in the skin of the abdomen, and collect the fluid with pipette. 5.Then adding 200μl DPBS with 2% FBS to the peritoneal cavity and aspirating the fluid. Repeating this steps for three times to collect more peritoneal cells. 6.Spin the collected cell suspension at 400 x g for 8 minutes, 7.Red blood cells were removed using the ACK Lysing Buffer 8.Centrifuge the lavage fluid of the peritoneal cells at 400 x g for 6 minutes and resuspending the cell pellet in 90 μl MACS Buffer which is prepared by dissolving bovine serum protein with auto MACS Rinsing Solution to form a 0.5% BSA solution. 9.Then the CD19 MicroBeads were added to the suspension, and the mixture was incubated at 4℃ for 10 minutes. 10.Applying the cell suspension onto the MS column placed in the magnetic field of a MACS Separator. And the labeled CD19 positive cells were retained on the column. 11.Collecting the positive selected cells for further research by removing the column from the magnetic field and flushing the column with MACS buffer. 12.The collected CD19 positive cells were filtered with 30-μm Cell Strainer and then were counted with the cell auto counter. 13.Centrifuging the cells and resuspending the cells with suitable volume buffer. (5’)GEM generation and reverse transcription1. GEMs are generated by combining barcoded Single Cell 5’ Gel Beads, a Master Mix with cells, and Partitioning Oil on a microfluidic chip. To achieve single cell resolution, the cells are delivered at a limiting dilution, such that the majority (~90 - 99%) of generated GEMs contains no cell, while the remainder largely contain a single cell. Immediately following generation of a GEM, the Single Cell 5’ Gel Bead is dissolved and any co-partitioned cell is lysed.2. Upon dissolution of the Single Cell 5’ Gel Bead in a GEM, oligonucleotides containing (i) an Illumina R1 sequence (read 1 sequencing primer), (ii) a 16 nt 10x Barcode, (iii) a 10 nt Unique Molecular Identifier (UMI), and (iv) a 13 nt Switch Oligo are released and mixed with cell lysate and a Master Mix that contains reverse transcription (RT) reagents and poly(dT) primers. Incubation of the GEMs then produces barcoded, full-length cDNA from poly-adenylated mRNA. After incubation, the GEMs are broken and the pooled post GEM-RT reaction mixtures are recovered.3. The pooled post GEM-RT reaction mixture contains barcoded first-strand cDNA from poly-adenylated mRNA, as well as leftover biochemical reagents and primers. Silane magnetic beads are used to purify the cDNA from this mixture. Barcoded, full-length V(D)J segments can then be directly enriched from the purified post GEM-RT product by TCR/Ig constant region-specific PCR amplification prior to library construction. Alternatively, the entire purified post GEM-RT product can be amplified by PCR and aliquoted prior to enriching for barcoded, full-length V(D)J segments. (5' sc-RNA-seq) library construction Firstly, Perform the cDNA amplification, and clean up the products. Fragmentation, End Repair & A-tailing 1. Dispense the appropriate volume for 50 ng into each well of a PCR 8-tube strip on a chilled metal block resting on ice. 2. Prepare a thermal cycler with the following incubation protocol and initiate the 4°C precool block step prior to assembling the Fragmentation Mix. The incubation protocol is: 4 °C holding, 32 °C for 2 min, 65 °C for 30 min, 4 °C holding. 3. Vortex the Fragmentation Buffer. Prepare the Fragmentation Mix on ice. Add the reagents in the order (Nuclease-free water, Fragmentation Buffer, Fragmentation Enzyme Blend). Pipette mix thoroughly and centrifuge briefly. 4. Dispense 30 µl Fragmentation Mix into each into tube containing 20 µl cDNA Amplification Product. Pipette mix 15 times on ice and centrifuge briefly before returning the tube strip to a chilled metal block resting on ice. 5. Transfer the chilled tube strip into the pre-cooled thermal cycler (4°C) and press “SKIP” to initiate the Fragmentation protocol. Post Fragmentation, End Repair & A-tailing Double Sided Size Selection - SPRIselect 6. Vortex the SPRIselect Reagent until fully resuspended. Add 30 μl SPRIselect Reagent (0.6X) to each sample in the tube strip and pipette mix 15 times. 7. Incubate the tube strip at room temperature for 5 min. 8. Place the tube strip in a 10x™ Magnetic Separator in the High position until the solution is clear. 9. Transfer 75 μl supernatant to a new tube strip and discard the previous tube strip. 10. Add 10 μl SPRIselect Reagent (0.8X) to each sample in the tube strip and pipette mix 15 times. 11. Incubate the tube strip at room temperature for 5 min. 12. Place the tube strip in a 10x Magnetic Separator in the High position until the solution is clear. 13. Carefully remove and discard 80 μl supernatant. 14. With the tube strip still in a 10x Magnetic Separator, add 125 µl 80% ethanol to the pellet and stand for 30 sec. Carefully remove and discard the ethanol wash. Repeat this step. 15. Briefly centrifuge the tube strip and return it to the 10x Magnetic Separator in the low position. Carefully remove and discard the remaining ethanol wash. 16. Remove the tube strip from the 10x Magnetic Separator and add 50.5 µl Buffer EB. Pipette mix 15 times. 17. Incubate the tube strip at room temperature for 2 min. 18. Place the tube strip in a 10x Magnetic Separator in the High position until the solution is clear. 19. Transfer 50 µl of sample to a new tube strip and cap the sample wells. Adaptor Ligation 20. Prepare Adaptor Ligation Mix by adding the reagents in the order (Nuclease-Free Water, Ligation Buffer, DNA Ligase, Adaptor Mix) Mix thoroughly and centrifuge briefly. 21. Add 50 µl Adaptor Ligation Mix to each tube containing 50 µl sample from the Fragmentation, End Repair and A-tailing Reaction. Pipette mix 15 times and centrifuge briefly. 22. Incubate in a thermal cycler at 20 °C for 15 min. Post Ligation Cleanup - SPRIselect 23. Vortex the SPRIselect Reagent until fully resuspended. Add 80 μl SPRIselect Reagent (0.8X) to each sample in the tube strip and pipette mix 15 times. 24. Incubate the tube strip at room temperature for 5 min. 25. Place the tube strip in a 10x™ Magnetic Separator in the High position until the solution is clear. Carefully remove and discard the supernatant. 26. Add 200 µl 80% ethanol to the pellet and stand for 30 sec. 27. Carefully remove and discard the ethanol wash. Repeat this step. 28. Centrifuge the tube strip briefly and return it to a 10x Magnetic Separator in the low position. 29. Remove and discard any remaining ethanol and allow the samples to air dry for 2 min. 30. Remove the tube strip from the 10x Magnetic Separator and add 30.5 µl Buffer EB. Pipette mix 15 times. 31. Incubate the tube strip at room temperature for 2 min. 32. Place the tube strip in a 10x Magnetic Separator in the Low position until the solution is clear. 33. Transfer 30 µl of sample to a new tube strip. Sample Index PCR 34. Prepare Sample Index PCR Mix by adding the reagents in the order (Nuclease-Free Water, Amplification Master Mix, SI-PCR Primer). Mix thoroughly and centrifuge briefly. 35. Add 60 µl Sample Index PCR Mix to each tube containing 30 µl purified Post Ligation sample. 36. Add 10 µl of an individual Chromium i7 Sample Index to each well and record their assignment. Pipette mix 5 times (pipette set to 90 μl) and centrifuge briefly. 37. Index the library DNA in a thermal cycler with the following protocol. The protocol is: 98 °C for 45 s, 6 cycles of 98 °C for 20 s, 54 °C for 30 s, and 72 °C for 20s, 72 °C for 1 min, and 4 °C holding. 38. Clean up the sample index PCR product with SPRIselect Reagent. 39. Perform the quality control of the cDNA library.

text: neonatal

text: wild type genotype

text: pool of 7

text: Mus musculus

text: Mus musculus

text: peritoneal cavity fluid

text: Mus musculus

text: male and female

characteristic: sex

characteristic: strain

text: D scRNA-seq