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text: thorax

text: Drosophila melanogaster

text: female

text: PromE800-GAL4, UAS-unc84GFP


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA54_Female_oenocyteGFP_adult_5dWT_Jasper_sample1_S38

characteristic: Submitter Id

text: ArrayExpress

text: oenocyte

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: thorax

text: Drosophila melanogaster

text: female

text: PromE800-GAL4, UAS-unc84GFP


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA54_Female_oenocyteGFP_adult_5dWT_Jasper_sample1_S39

characteristic: age

text: ArrayExpress

characteristic: broker name

text: oenocyte

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: thorax

text: Drosophila melanogaster

text: female

text: PromE800-GAL4, UAS-unc84GFP


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA62_Female_ovary_adult_5dWT_Nystul_All_Nuclei_S58

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

characteristic: organism part

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA62_Female_ovary_adult_5dWT_Nystul_All_Nuclei_S62

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

text: Drosophila melanogaster

text: female


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA62_Female_ovary_adult_5dWT_Nystul_All_Nuclei_S63

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

text: Drosophila melanogaster

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA63_Female_ovary_adult_5dWT_Nystul_Big_Nuclei_S66

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: ovary

text: Drosophila melanogaster

text: femal


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA63_Female_ovary_adult_5dWT_Nystul_Big_Nuclei_S71

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

characteristic: organism part

text: Drosophila melanogaster

text: femal

characteristic: sex

characteristic: strain

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA63_Female_ovary_adult_5dWT_Nystul_Big_Nuclei_S72

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: ovary

text: Drosophila melanogaster

text: femal


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA51_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S25

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA51_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S37

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA51_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S40

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

characteristic: organism part

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA52_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S27

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA52_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S32

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: female


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA52_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample2_S36

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA41_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S59

text: ArrayExpress

text: fruit fly

text: Protocols: tissue dissection 10x Genomics v3 chemistry 10x Genomics v3 chemistry

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

characteristic: organism part

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: VIB Center for Brain & Disease Research, KU Leuven, Leuven 3000, Belgium

text: public

text: E-MTAB-10519:FCA41_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S71

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA41_Male_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S72

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA42_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S56

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: female


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA42_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S57

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

characteristic: organism part

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: strain

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA42_Female_proboscis_and_maxpalp_adult_5dWT_Scott_sample1_S66

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: mixed proboscis and maxillary palpus

text: Drosophila melanogaster

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA59_Male_testis_adult_1dWT_Fuller_sample1_S48

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA59_Male_testis_adult_1dWT_Fuller_sample1_S49

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA59_Male_testis_adult_1dWT_Fuller_sample1_S56

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA60_Male_testis_adult_1dWT_Fuller_sample2_S61

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA60_Male_testis_adult_1dWT_Fuller_sample2_S67

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA60_Male_testis_adult_1dWT_Fuller_sample2_S70

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA61_Male_testis_adult_1dWT_Fuller_sample3_S65

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

characteristic: organism part

text: Drosophila melanogaster

text: male

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA61_Male_testis_adult_1dWT_Fuller_sample3_S68

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA61_Male_testis_adult_1dWT_Fuller_sample3_S69

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: testis

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA57_Male_TracheaGFP_adult_5dWT_Kornberg_sample1_S43

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

text: Drosophila melanogaster

text: male

text: Btl-GAL4, UAS-lamGFP


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA57_Male_TracheaGFP_adult_5dWT_Kornberg_sample1_S47

text: ArrayExpress

text: fruit fly

text: Protocols: tissue dissection 10x Genomics v3 chemistry 10x Genomics v3 chemistry

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: Drosophila melanogaster

text: male

characteristic: sex

text: Btl-GAL4, UAS-lamGFP

characteristic: strain

characteristic: title


text: VIB Center for Brain & Disease Research, KU Leuven, Leuven 3000, Belgium

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA57_Male_TracheaGFP_adult_5dWT_Kornberg_sample1_S55

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: Drosophila melanogaster

text: male

text: Btl-GAL4, UAS-lamGFP


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA58_Female_TracheaGFP_adult_5dWT_Kornberg_sample1_S45

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

text: Drosophila melanogaster

text: female

text: Btl-GAL4, UAS-lamGFP


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA58_Female_TracheaGFP_adult_5dWT_Kornberg_sample1_S50

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: trachea

text: Drosophila melanogaster

text: female

text: Btl-GAL4, UAS-lamGFP


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA58_Female_TracheaGFP_adult_5dWT_Kornberg_sample1_S51

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: trachea

characteristic: organism part

text: Drosophila melanogaster

text: female

characteristic: sex

text: Btl-GAL4, UAS-lamGFP

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA39_Male_wing_adult_5dWT_Scott_sample1_S42

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: wing

text: Drosophila melanogaster

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA39_Male_wing_adult_5dWT_Scott_sample1_S43

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: wing

text: Drosophila melanogaster

text: male


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10519:FCA39_Male_wing_adult_5dWT_Scott_sample1_S50

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: adult

text: wild type genotype

characteristic: individual

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: wing

text: Drosophila melanogaster

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10519:FCA40_Female_wing_adult_5dWT_Scott_sample1_S37

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: wing

characteristic: organism part

text: Drosophila melanogaster

text: female

characteristic: sex

characteristic: strain

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10519:FCA40_Female_wing_adult_5dWT_Scott_sample1_S39

text: ArrayExpress

text: fruit fly

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: wing

text: Drosophila melanogaster

text: female


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10519:FCA40_Female_wing_adult_5dWT_Scott_sample1_S49

characteristic: Submitter Id

text: ArrayExpress

text: fruit fly

text: adult

text: wild type genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: wing

text: Drosophila melanogaster

text: female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Helmholtz Center Munich

text: public

tag: Namespace:Helmholtz Center Munich

characteristic: age

text: ArrayExpress

characteristic: broker name

text: guard cells

text: Barke

characteristic: cultivar

text: Protocols: Epidermal peels were collected from the abaxial side of 8 to 10-day-old leaves. To prepare isolated epidermal peels, leaves were cut from the plant and bent over the forefinger with the adaxial surface facing upward. A shallow cut was made with a sharp razor blade horizontally across the leaf and a flap of leaf tissue lifted with a razor, leaving the lower epidermis intact. The leaf tissue was removed from the epidermis with forceps. Samples were taken from whole leaves epidermal peels and highly GC enriched tissue. Epidermal peels from 12-day-old Barley (cv. Barke) leaves were isolated. Thereby only the GC SC complex survives. For GC samples we used the ‘blender method’ on epidermal peels with mature, intact GCs to mechanically and selectively destroy the SCs while keeping GCs alive. GC were enriched within 8 minutes by successive blender cycles (45 s each) in ice-cold deionized water with additional crushed ice and filtered through a 210-mm nylon mash. After two rounds of blending, the remaining light green epidermal fraction was further processed. Neutral red staining indicated that at least 90% of the viable cells in the preparations were GCs. Barley (Hordeum vulgare cv. Barke) seeds were provided by a commercial supplier (Saatzucht J. Breun GmbH & Co. KG) and cultivated at 22/16C and 50 ± 5% RH at a 12/12h day/night cycle and a photon flux density of 500 mmol m-2 s-1 white light (Philips Master T Green Powers, 400 W). RNA was extracted from a total of 20 epidermal peels per sample using the NucleoSpin RNA Plant Kit (Macherey-Nagel,Drueren, Germany). RNA isolation from whole leaves was performed similarly. The extracted RNA was treated with RNase-free DNase (New England Biolabs, Ipswich, MA, USA). Quality control measurements were performed on a 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and the concentration was determined using a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). Libraries were prepared with the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) using 1 mg of RNA.

text: LP.03 three leaves visible stage

text: wild type genotype

text: Hordeum vulgare

characteristic: organism

text: Hordeum vulgare

text: leaf abaxial epidermis

characteristic: sampling time

text: Hordeum vulgare


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Center Munich

text: public

text: ArrayExpress

text: guard cells; subsidiary cells

text: Barke

text: LP.03 three leaves visible stage

text: wild type genotype

text: Hordeum vulgare

text: Hordeum vulgare

text: leaf abaxial epidermis

characteristic: organism part

text: Hordeum vulgare

characteristic: title


text: Helmholtz Center Munich

characteristic: INSDC center name

text: public

text: 10 to 12

text: ArrayExpress

text: whole epidermal peel

text: Barke

characteristic: description

text: LP.02 two leaves visible stage

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Hordeum vulgare

text: Hordeum vulgare

text: leaf abaxial epidermis

text: 8 to 10

text: Hordeum vulgare


text: NEWCASTLE UNIVERSITY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:NEWCASTLE UNIVERSITY

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Samples were collected by the Human Developmental Biology Resource and subsequently dissected by the Haniffa lab team. Single cells suspensions were made by mechanical digestion and live, enriched cells were selected by FACS. RNA was extracted by cell lysis during droplet encapsulation on board the 10x Chromium Controller and purified after Reverse Transcription using DynaBeads. Libraries were constructed after cDNA amplification by fragmentation and ligation of adapter sequences before a final index PCR adding sample specific barcodes and sequencing adapters.

text: embryo stage

text: carnegie stage 18

characteristic: embryo stage

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: liver

text: liver

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: embryo stage

text: carnegie stage 18

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: yolk sac

text: membrane

text: Homo sapiens

text: male


text: NEWCASTLE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

characteristic: description

text: embryo stage

characteristic: developmental stage

text: carnegie stage 18

text: Homo sapiens

text: Homo sapiens

text: yolk sac

characteristic: organism part

text: vitelline blood vessel

text: Homo sapiens

text: male

characteristic: title


text: NEWCASTLE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: human

characteristic: common name

text: embryo stage

text: carnegie stage 18

characteristic: embryo stage

text: Hep

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: liver

text: liver

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

text: COVID-19 Genomics UK Consortium

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: receipt date

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Northumbria University / South Tees Hospitals NHS Foundation Trust / North Cumbria Integrated Care NHS Foundation Trust / North Tees and Hartlepool NHS Foundation Trust / Newcastle Hospitals NHS Foundation Trust

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


SAMI00000007-TEST AGAINaasda

text: KLE TEch

text: public

text: SAMI00000007-TEST AGAINaasda

tag: Namespace:KLE TEch

text: rice

characteristic: common name

text: TEST AGAIN AGAINadsdsasd

characteristic: description

text: Oryza sativa

text: Oryza sativa

text: TEST AGAINaasda


text: oesophagus epithelium

characteristic: ArrayExpress-Phenotype

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: oesophagus epithelium

characteristic: ArrayExpress-PHENOTYPE

text: Mus musculus

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

text: oesophagus epithelium

characteristic: PHENOTYPE

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

text: public

tag: Namespace:Quadram Institute Bioscience

text: COVID-19 Genomics UK Consortium

text: Quadram Institute Bioscience

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCNEDY

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCNEDY

tag: Namespace:University of Birmingham

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCNEDY

characteristic: title

text: not provided


text: public

tag: Namespace:Virology Department, Sheffield Teaching Hospitals NHS Foundation Trust/Department of Infection, Immunity and Cardiovascular Disease, The Medical School, University of Sheffield

text: COVID-19 Genomics UK Consortium

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided


COG-UK/BIRM-BCNGRY

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCNGRY

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCNGRY

text: not provided

characteristic: virus identifier


text: Virology Department, Sheffield Teaching Hospitals NHS Foundation Trust/Department of Infection, Immunity and Cardiovascular Disease, The Medical School, University of Sheffield

text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided


text: public

tag: Namespace:Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


E-MTAB-10480:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10480:Sample 1

tag: Namespace:Neuroscience and Mental Health Research Institute, Cardiff University

characteristic: Submitter Id

text: ArrayExpress

text: induced pluripotent stem cell

text: human

characteristic: common name

text: Protocols: The stem cells were gifted from: Lieber Institute for Brain Development, Johns Hopkins Medical Campus. All relevant information including the cat no. https://www.atcc.org/products/crl-2522. Total RNA was extracted from a control hiPSC line and control cells treated for 72h with various concentrations of UNC0638 ..e. 50, 100, 200 or 250nM as a model of Kleefstra syndrome. Total RNA was extratced using the miRNeasy mini kit (Qiagen, Germany) following manufacturer's instructions. Polyadenylated adaptors were ligated to the 3′-end, 5′-adaptors were then ligated, and the resulting RNAs were reverse transcribed to generate cDNA that can be amplified by PCR. The amplified product was run on 3% certified™ low range ultra agarose (Bio-Rad Laboratories Ltd) in TBE buffer and a size-selection was performed to ensure that the cDNA used for sequencing primarily contains miRNAs rather than other RNA contaminants. The gel containing the required cDNAs was purified using QIAquick Gel Extraction Kit (Qiagen, Germany). Quality and quantity of miRNA libraries were assessed by Bioanalyzer (Agilent Technologies) and by Qubit (Thermo Fisher Scientific).

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

characteristic: organism part

text: fibroblast

characteristic: progenitor cell type

text: Homo sapiens

text: male

characteristic: sex

text: Sample 1

characteristic: title


E-MTAB-10480:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Neuroscience and Mental Health Research Institute, Cardiff University

text: public

text: E-MTAB-10480:Sample 2

text: ArrayExpress

characteristic: broker name

text: induced pluripotent stem cell

text: human

text: adult

text: normal

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: skin

text: fibroblast

text: Homo sapiens

text: male

text: Sample 2


E-MTAB-10480:Sample 4

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10480:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: induced pluripotent stem cell

text: human

text: adult

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

text: fibroblast

text: Homo sapiens

text: male

text: Sample 4


E-MTAB-10480:Sample 3

text: public

text: E-MTAB-10480:Sample 3

text: ArrayExpress

text: induced pluripotent stem cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: skin

text: fibroblast

text: Homo sapiens

text: male

text: Sample 3


E-MTAB-10480:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-10480:Sample 5

text: ArrayExpress

text: induced pluripotent stem cell

text: human

text: adult

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: skin

characteristic: organism part

text: fibroblast

characteristic: progenitor cell type

text: Homo sapiens

text: male

characteristic: sex

text: Sample 5

characteristic: title


SAMI00000008-NEW API Title

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: KLE TEch

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SAMI00000008-NEW API Title

text: rice

text: Description new api

text: Oryza sativa

text: Oryza sativa

text: NEW API Title


COG-UK/BIRM-BCRRCQ

text: University of Birmingham

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRCQ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRRCQ

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRGBN

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRGBN

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRGBN

characteristic: title

text: not provided


text: public

text: COVID-19 Genomics UK Consortium

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided


COG-UK/BIRM-BCRRHP

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRHP

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRRHP

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRGGA

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRGGA

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRGGA

characteristic: title

text: not provided


COG-UK/BIRM-BCRRES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRES

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRRES

text: not provided


text: Originating lab: Wales Specialist Virology Centre Sequencing lab: Pathogen Genomics Unit

text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRGEW

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRGEW

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRGEW

characteristic: title

text: not provided


COG-UK/BIRM-BCRRYG

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRYG

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRRYG

text: not provided


COG-UK/BIRM-BCRGYR

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRGYR

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRGYR

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRRMO

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRMO

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRRMO

characteristic: title

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: not provided


text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRRTD

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRTD

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRRTD

characteristic: title

text: not provided


COG-UK/BIRM-BCRRBR

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRBR

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRRBR

text: not provided


COG-UK/BIRM-BCRROF

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRROF

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRROF

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRRJW

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRJW

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRRJW

characteristic: title

text: not provided


COG-UK/BIRM-BCRDHQ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRDHQ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRDHQ

text: not provided


COG-UK/BIRM-BCRRDY

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRDY

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRRDY

text: not provided

characteristic: virus identifier


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

characteristic: title

text: not provided


COG-UK/BIRM-BCRRNN

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRNN

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRRNN

text: not provided


COG-UK/BIRM-BCRGRH

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRGRH

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRGRH

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRRQK

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRQK

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRRQK

characteristic: title

text: not provided


COG-UK/BIRM-BCRRXE

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRXE

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRRXE

text: not provided


COG-UK/BIRM-BCRRPC

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRRPC

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: COG-UK/BIRM-BCRRPC

text: not provided

characteristic: virus identifier


COG-UK/BIRM-BCRRZZ

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Birmingham

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: COG-UK/BIRM-BCRRZZ

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

text: University of Birmingham

text: not provided

text: not provided

text: United Kingdom

text: England

text: not provided

text: not provided

text: Human

text: not provided

characteristic: host sex

text: not provided

text: not provided

characteristic: organism

text: not provided

text: COG-UK/BIRM-BCRRZZ

characteristic: title

text: not provided


COG-UK/BIRM-BCRGNY

text: University of Birmingham

text: public

text: COG-UK/BIRM-BCRGNY

text: COVID-19 Genomics UK Consortium

characteristic: broker name

text: University of Birmingham

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: receipt date

text: not provided

text: COG-UK/BIRM-BCRGNY

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Zurich, Department of Quantitative Biomedicine

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Fresh human breast tumor tissue was dissociated into single cells as previously described (Wagner et al., Cell 2019) 10x v3 protocol; Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075 10x v3 protocol; Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075

characteristic: description

text: adult

characteristic: developmental stage

text: Invasive Breast Carcinoma

characteristic: disease

characteristic: individual

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

characteristic: organism part

text: immune-environment 1 (exhausted)

characteristic: phenotype

text: Homo sapiens

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Zurich, Department of Quantitative Biomedicine

text: public

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: Invasive Breast Carcinoma

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: breast

text: immune-environment 1 (exhausted)

text: Homo sapiens

text: female

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: Invasive Breast Carcinoma

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: breast

text: immune-environment 2 (non-exhausted)

text: Homo sapiens

text: female


text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo1

tag: Namespace:The Francis Crick Institute

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: The VHL-defective kidney cancer cell lines, RCC4 and 786-O, were used. These cell lines were modified either by lentiviral transduction (to re-introduce VHL or an empty vector as control) and/or by CRISPR/Cas9-mediated genome editing of the EIF4E2 locus. Cells were grown on 15 cm dishes in DMEM with 1 mM sodium pyruvate, 10% FBS and 5 µg/mL blasticidin at 37 ˚C in 5% CO2. mTOR activity was inhibited by treating the cells with 250 nM of Torin 1 for 2 hours. mRNA translation was arrested by treating the cells with 100 µg/mL cycloheximide for 3 min prior to harvesting the cells. Cells were washed with ice-cold PBS with 100 µg/mL cycloheximide, and lysed using cytoplasmic lysis buffer. The cytoplasmic lysate was fractionated according to the number of associated ribosomes using sucrose density gradients and ultracentrifugation. RNA from the polysome fractionated samples was extracted using an RNA clean and concentrator-5 kit (Zymo Research, R1016) according to the manufacturer’s instructions, with the following modifications: 322.5 µL of preconditioned RNA binding buffer (157.5 µL of RNA binding buffer (supplied with RNA clean and concentrator-5 kit), 157.5 µL of absolute EtOH and 7.5 µL of 20% SDS) was added to 150 µL of the fractionated samples before loading to the column. 5′ end-Seq was performed on the extracted total RNA. In short, a full-length cDNA library was generated using a combined reverse transcription and template switching reaction, followed by PCR amplification. The amplified libraries were multiplexed, and then purified using ProNex beads (Promega). The 3′ end of the cDNA library (i.e. 5′ end of mRNAs) was amplified specifically using Tn5 transposase-mediated tagmentation and PCR amplification with primers annealing to the appropriate adapters added by the template switching reaction and tagmentation. The PCR primers contained i5 and i7 Illumina index sequences and the amplified libraries were multiplexed.

characteristic: description

text: adult

characteristic: developmental stage

text: renal cell adenocarcinoma

characteristic: disease

text: ribopolysome fraction 1

characteristic: fraction

text: VHL lentivirus

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo2

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 2

text: VHL lentivirus

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo3

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 3

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

characteristic: title


text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo4

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: renal cell adenocarcinoma

characteristic: disease

text: ribopolysome fraction 4

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo5

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 5

characteristic: fraction

text: VHL lentivirus

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

characteristic: stimulus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo6

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 6

text: VHL lentivirus

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


text: The Francis Crick Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo7

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 7

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g1_1_NA_ribo8

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: renal cell adenocarcinoma

characteristic: disease

text: ribopolysome fraction 8

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g2_1_NA_ribo1

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 1

characteristic: fraction

text: VHL lentivirus

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

characteristic: stimulus


text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g2_1_NA_ribo2

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 2

text: VHL lentivirus

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Francis Crick Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g2_1_NA_ribo3

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 3

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g2_1_NA_ribo4

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: renal cell adenocarcinoma

characteristic: disease

text: ribopolysome fraction 4

text: VHL lentivirus

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10689:polysome_786O_VHL_noEIF4E2_g2_1_NA_ribo5

text: ArrayExpress

text: human

text: adult

text: renal cell adenocarcinoma

text: ribopolysome fraction 5

characteristic: fraction

text: VHL lentivirus

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

characteristic: stimulus