text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i707_i511.HK3H3BBXY.s_2

tag: Namespace:Helmholtz Munich

characteristic: Submitter Id

text: 74 to 80

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i707_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i707_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i707_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i707_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i707_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i707_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i710_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i710_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i710_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i710_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i710_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i710_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i710_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i710_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i710_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i710_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i710_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i711_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i711_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i711_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i711_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i711_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i711_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i711_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i711_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i711_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i711_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i711_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i712_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i712_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i712_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i712_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i712_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i712_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i712_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i712_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i712_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i712_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i712_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i714_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i714_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i714_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i714_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i714_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i714_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i714_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i714_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i714_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i714_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i714_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i715_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i715_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i715_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i715_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i715_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i715_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i715_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i715_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i715_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i715_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i715_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i716_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i716_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i716_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i716_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: not OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i716_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i716_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i716_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i716_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i716_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i716_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i716_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i502.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i718_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i718_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i506.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i718_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i718_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i510.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i718_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i718_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i515.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i718_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i718_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i518.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105lo

text: balanced hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i718_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105lo

characteristic: immunophenotype

text: balanced hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i718_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i718_i522.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i719_i503.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i719_i505.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i719_i507.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i719_i508.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i719_i511.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i719_i513.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i719_i516.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i719_i517.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i719_i520.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i719_i521.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i719_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i502.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i720_i503.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i720_i505.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i506.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i720_i507.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i720_i508.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i510.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i720_i511.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: Sema4A -/-

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i720_i513.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i515.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i720_i516.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i720_i517.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i518.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK


text: Helmholtz Munich

text: public

characteristic: SRA accession

text: E-MTAB-11359:SLX-19432.i720_i520.HK3H3BBXY.s_2

characteristic: Submitter Id

text: 74 to 80

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: not OK

characteristic: title


text: Helmholtz Munich

text: public

text: E-MTAB-11359:SLX-19432.i720_i521.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: wild type genotype

characteristic: genotype

text: LKS; CD34-; CD105hi

characteristic: immunophenotype

text: myeloid-biased hematopoietic stem cell

characteristic: inferred cell type

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

characteristic: organism part

text: aged

characteristic: phenotype

characteristic: protocol

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Helmholtz Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11359:SLX-19432.i720_i522.HK3H3BBXY.s_2

text: 74 to 80

text: ArrayExpress

text: mononuclear cell

text: house mouse

text: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.

text: wild type genotype

text: LKS; CD34-; CD105hi

text: myeloid-biased hematopoietic stem cell

text: Mus musculus

text: Mus musculus

text: bone marrow

text: aged

text: Mus musculus

text: OK