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text: Protocols: Human fibroblast HS-27, TIG-1 and CRL-7815 cells were cultured within 1640 (Thermo Fisher Scientific). Complete culture medium contained 10% fetal bovine serum and 100 U/mL penicillin and streptomycin (Thermo Fisher Scientific). Total RNA of 1 × 10^7 cells were extracted using TRIZOL (Thermo Fisher Scientific). Nanodrop 2000 (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100 (Agilent) were used to evaluate RNA concentration and quality. Four μg of RNA from different experimental groups was prepared for cDNA library using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions.

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