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text: E-MTAB-11873:Sample 1

tag: Namespace:THE JACKSON LABORATORY

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: dendritic cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Bone-marrow cells from naïve mice were isolated by flushing femurs, tibias and humeri with RPMI-1640 medium. Following suspended in ACK lysis buffer to lyse red blood cells, bone marrow cells were passed through a 40 µm filter. Then cells were resuspended and cultured in complete RPMI-1640 medium containing GM-CSF and IL-4. The cultured medium was entirely discarded on day 4 and new warmed medium supplemented with GM-CSF and IL-4 was added. On day 7, the cultured cells were collected and used for further experiments. BM-DCs were incubated with WT or Ptgs2-/- lung fibroblast-derived conditioned medium or purified PGE2. Total RNA was isolated from BM-DCs using the miRNeasy Mini kit (Qiagen). Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit: isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. Libraries were checked for quality and concentration using the DNA 1000 assay and quantitative PCR, following the manufacturers' instructions.

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: monoculture

characteristic: growth condition

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: stimulus

characteristic: strain

text: Sample 1


E-MTAB-11873:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE JACKSON LABORATORY

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11873:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: dendritic cell

text: house mouse

text: adult

text: wild type genotype

text: monoculture

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 2

characteristic: title


E-MTAB-11873:Sample 3

text: THE JACKSON LABORATORY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-11873:Sample 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: dendritic cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: co-culture with wild type lung fibroblasts

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 3


E-MTAB-11873:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE JACKSON LABORATORY

characteristic: INSDC first public

text: public

text: E-MTAB-11873:Sample 4

text: ArrayExpress

text: dendritic cell

text: house mouse

text: adult

text: wild type genotype

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: stimulus

characteristic: strain

text: Sample 4


E-MTAB-11873:Sample 5

text: THE JACKSON LABORATORY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11873:Sample 5

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: dendritic cell

text: house mouse

text: adult

text: wild type genotype

text: co-culture with Ptgs2 -/- lung fibroblasts

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 5

characteristic: title


E-MTAB-11873:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE JACKSON LABORATORY

text: public

text: E-MTAB-11873:Sample 6

text: ArrayExpress

text: dendritic cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 6


E-MTAB-11873:Sample 7

text: THE JACKSON LABORATORY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11873:Sample 7

text: ArrayExpress

text: dendritic cell

text: house mouse

text: adult

text: wild type genotype

text: monoculture

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: stimulus

characteristic: strain

text: Sample 7


E-MTAB-11873:Sample 8

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: THE JACKSON LABORATORY

text: public

text: E-MTAB-11873:Sample 8

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: dendritic cell

text: house mouse

text: adult

text: wild type genotype

text: monoculture

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 8

characteristic: title


text: UK Health Security Agency

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-27-06-2022-11:54:30:845-39874

tag: Namespace:UK Health Security Agency

characteristic: collection date

text: Invasive isolate

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: M22 240102

text: invasive

characteristic: isolation source

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UK Health Security Agency

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-27-06-2022-11:54:30:845-39875

text: Invasive isolate

text: United Kingdom

text: diseased

text: Homo sapiens

text: M22 240085

text: invasive

text: Neisseria meningitidis

text: Neisseria meningitidis


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

text: public

tag: Namespace:Tree of Life Programme

characteristic: Submitter Id

text: JOHN WANG

characteristic: collected by

text: ACADEMIA SINICA

characteristic: collecting institution

text: Thailand

text: not collected

unit: DD

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: KHAO YAI NATIONAL PARK

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: NOT COLLECTED

characteristic: identified by

text: NOT COLLECTED

text: not applicable

characteristic: lifestage

text: Caenorhabditis sp. BRC20454

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Caenorhabditis sp. BRC20454

text: NOT APPLICABLE

characteristic: sex

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: symbiont

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOHN WANG

text: ACADEMIA SINICA

characteristic: collection date

text: Solomon Islands

characteristic: geographic location (country and/or sea)

text: not collected

text: not collected

text: no further information

text: NOT COLLECTED

text: NOT COLLECTED

text: NOT COLLECTED

text: not applicable

text: Caenorhabditis sp. BRC20456

text: WHOLE ORGANISM

text: ToL

text: Caenorhabditis sp. BRC20456

text: NOT APPLICABLE

text: NOT PROVIDED

text: N

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOHN WANG

characteristic: collected by

text: ACADEMIA SINICA

characteristic: collecting institution

text: Taiwan

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: TAPEI CITY

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: NOT COLLECTED

characteristic: identified by

text: NOT COLLECTED

text: not applicable

characteristic: lifestage

text: Caenorhabditis sp. BRC20494

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

characteristic: project name

text: Caenorhabditis sp. BRC20494

text: NOT APPLICABLE

characteristic: sex

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: YEN-PING HSUEH

text: ACADEMIA SINICA

characteristic: collection date

text: Taiwan

text: not collected

text: not collected

text: MIALOLI COUNTY

text: NOT COLLECTED

text: NOT COLLECTED

text: NOT COLLECTED

text: not applicable

text: Caenorhabditis sp. BRC20483

text: WHOLE ORGANISM

text: ToL

text: Caenorhabditis sp. BRC20483

text: NOT APPLICABLE

text: NOT PROVIDED

text: N

characteristic: symbiont

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOHN WANG

characteristic: collected by

text: ACADEMIA SINICA

characteristic: collecting institution

text: Thailand

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: KHAO YAI NATIONAL PARK

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: NOT COLLECTED

characteristic: identified by

text: NOT COLLECTED

text: not applicable

characteristic: lifestage

text: Caenorhabditis sp. BRC20454

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: project name

characteristic: sample same as

text: Caenorhabditis sp. BRC20454

text: NOT APPLICABLE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOHN WANG

text: ACADEMIA SINICA

characteristic: collection date

text: Solomon Islands

text: not collected

text: not collected

text: no further information

text: NOT COLLECTED

text: NOT COLLECTED

text: NOT COLLECTED

text: not applicable

text: Caenorhabditis sp. BRC20456

text: WHOLE ORGANISM

text: Caenorhabditis sp. BRC20456

text: NOT APPLICABLE

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: symbiont


text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOHN WANG

characteristic: collected by

text: ACADEMIA SINICA

characteristic: collecting institution

text: Taiwan

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: TAPEI CITY

characteristic: geographic location (region and locality)

text: NOT COLLECTED

characteristic: habitat

text: NOT COLLECTED

characteristic: identified by

text: NOT COLLECTED

text: not applicable

characteristic: lifestage

text: Caenorhabditis sp. BRC20494

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: project name

characteristic: sample same as

text: Caenorhabditis sp. BRC20494

text: NOT APPLICABLE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: YEN-PING HSUEH

text: ACADEMIA SINICA

characteristic: collection date

text: Taiwan

text: not collected

text: not collected

text: MIALOLI COUNTY

text: NOT COLLECTED

text: NOT COLLECTED

text: NOT COLLECTED

text: not applicable

text: Caenorhabditis sp. BRC20483

text: WHOLE ORGANISM

text: Caenorhabditis sp. BRC20483

text: NOT APPLICABLE

characteristic: specimen id

text: NOT PROVIDED

characteristic: specimen voucher

text: N

characteristic: title


text: Liverpool John Moores University

text: public

tag: Namespace:Liverpool John Moores University

text: not provided

text: human faecal sample from healthy (non-CRC) individual

characteristic: description

text: not provided

characteristic: environment (biome)

text: not provided

characteristic: environment (feature)

text: not provided

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: human gut metagenome

characteristic: organism

text: The active meta-transcriptome in colorectal cancer

characteristic: project name

text: human gut metagenome

text: Illumina

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Liverpool John Moores University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

text: human faecal sample from an individual with colorectal cancer (CRC), pre-surgery

text: not provided

text: not provided

text: not provided

text: United Kingdom

text: not provided

text: not provided

text: human-gut

text: metagenome

text: human gut metagenome

text: human gut metagenome

text: Illumina


text: Liverpool John Moores University

text: public

text: not provided

characteristic: collection date

text: human faecal sample from an individual with colorectal cancer (CRC), post-surgery

characteristic: description

text: not provided

characteristic: environment (biome)

text: not provided

characteristic: environment (feature)

text: not provided

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

text: human-gut

text: metagenome

text: human gut metagenome

text: human gut metagenome

text: Illumina

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Anthony Smith, NICD

text: public

tag: Namespace:Anthony Smith, NICD

text: EnteroBase

characteristic: broker name

text: EnteroBase

text: A. Smith, National Institute for Communicable Diseases

characteristic: collected by

text: Whole genome sequencing data uploaded by EnteroBase user

text: South Africa, Western Cape

characteristic: geographic location

text: Human, Blood culture

characteristic: isolation source

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Enteritidis

characteristic: serovar

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: EnteroBase

text: EnteroBase

characteristic: collection date

text: South Africa, Western Cape

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Anthony Smith, NICD

text: public

text: EnteroBase

text: EnteroBase

characteristic: description

text: South Africa, Western Cape

text: Human, Stool

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis

characteristic: title


text: Anthony Smith, NICD

text: public

text: EnteroBase

characteristic: broker name

text: EnteroBase

characteristic: collected by

text: South Africa, Gauteng

characteristic: geographic location

text: Human, Blood culture

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Enteritidis