text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Patescibacteria group bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Patescibacteria group bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

characteristic: Submitter Id

characteristic: assembly quality

text: MEGAHIT; kmer set 29,49,69,89,109,119,129,149

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

text: Patescibacteria assembled from Lake Most

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Czech Republic

characteristic: geographic location (country and/or sea)

unit: DD

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: uncultured Candidatus Saccharimonas sp.

text: uncultured Candidatus Saccharimonas sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


text: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO

text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharimonas sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Candidatus Saccharimonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Nomurabacteria bacterium

text: Candidatus Nomurabacteria bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Gracilibacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Gracilibacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

text: Patescibacteria assembled from Rimov Reservoir

characteristic: description

text: freshwater lake

text: reservoir

text: fresh water

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Nomurabacteria bacterium

text: Candidatus Nomurabacteria bacterium

text: Illumina HiSeq 4000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: reservoir

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharimonas sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Candidatus Saccharimonas sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: reservoir

text: fresh water

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: uncultured Candidatus Saccharimonas sp.

text: uncultured Candidatus Saccharimonas sp.

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: reservoir

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Patescibacteria group bacterium UBA4664

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Patescibacteria group bacterium UBA4664

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: reservoir

text: fresh water

text: Czech Republic

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Patescibacteria group bacterium UBA4664

text: Patescibacteria group bacterium UBA4664

text: Illumina HiSeq 4000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: reservoir

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Czech Republic

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Nomurabacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Nomurabacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

text: Patescibacteria assembled from Lake Thun

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Taylorbacteria bacterium

text: Candidatus Taylorbacteria bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Nomurabacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Nomurabacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Patescibacteria group bacterium UBA4664

text: Patescibacteria group bacterium UBA4664

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Peribacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Peribacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: candidate division WWE3 bacterium

text: candidate division WWE3 bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Uhrbacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Uhrbacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Parcubacteria group bacterium

text: Parcubacteria group bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Levybacteria bacterium GW2011_GWA1_37_16

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Patescibacteria group bacterium

text: Patescibacteria group bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Uhrbacteria bacterium RIFOXYB12_FULL_58_10

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

text: Patescibacteria assembled from Lake Traunsee

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Austria

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Nomurabacteria bacterium

text: Candidatus Nomurabacteria bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Austria

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Uhrbacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Uhrbacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Austria

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Parcubacteria group bacterium

text: Parcubacteria group bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: Patescibacteria assembled from Lake Zurich

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Nomurabacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Nomurabacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Nomurabacteria bacterium

text: Candidatus Nomurabacteria bacterium

text: Illumina HiSeq 4000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Berkelbacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Berkelbacteria bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Parcubacteria group bacterium

text: Parcubacteria group bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Illumina HiSeq 4000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Patescibacteria group bacterium UBA4664

text: Patescibacteria group bacterium UBA4664

text: Illumina HiSeq 4000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Nomurabacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Nomurabacteria bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

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text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Patescibacteria group bacterium UBA4664

text: Patescibacteria group bacterium UBA4664

text: Illumina HiSeq 2500

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Taylorbacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Taylorbacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

text: coverage and kmer

characteristic: collection date

characteristic: completeness score

text: checkm

characteristic: completeness software

characteristic: contamination score

text: freshwater lake

characteristic: environment (biome)

text: lake

characteristic: environment (feature)

text: fresh water

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: Freshwater lake

text: freshwater metagenome

characteristic: metagenomic source

text: Candidatus Magasanikbacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Candidatus Magasanikbacteria bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

characteristic: assembly software

text: coverage and kmer

characteristic: binning parameters

characteristic: binning software

text: checkm

characteristic: description

text: freshwater lake

text: lake

text: fresh water

text: Switzerland

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: Freshwater lake

text: freshwater metagenome

text: Candidatus Peribacteria bacterium

text: Candidatus Peribacteria bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach


text: public

tag: Namespace:Helmholtz Centre for Environmental Research GmbH - UFZ

text: electroactive Geobacter dominated biofilm

text: bioanode metagenome

characteristic: organism

text: bioanode metagenome

text: pre-culture 1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Helmholtz Centre for Environmental Research GmbH - UFZ

text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 1.1 (pre1)


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 2.1 (pre1)


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 3.1 (pre1)


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: bioanode metagenome

text: bioanode metagenome

text: pre-culture 2.a


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 1.2 (pre2.a)


text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 2.2 (pre2.a)


text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-CA 3.2 (pre2.a)


text: public

text: electrochemical flow cell biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EFC-2BES (pre2.a)


text: public

text: electrochemical flow cell biofilm OCP control

text: bioanode metagenome

text: bioanode metagenome

text: EFC-OCP 1 (pre2.a)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EFC-OCP 2 (pre2.a)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: pre-culture 2.b


text: public

text: electrochemical batch reactor biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 1 (pre2.b)


text: public

text: electrochemical batch reactor biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 2 (pre2.b)


text: public

text: electrochemical batch reactor biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 3 (pre2.b)


text: public

text: electrochemical batch reactor biofilm

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 4 (pre2.b)


text: public

text: electrochemical batch reactor biofilm OCP control

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 1 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 2 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 3 (pre2.b)


text: public

text: electroactive Geobacter dominated biofilm mcrA

text: bioanode metagenome

text: bioanode metagenome

text: pre-culture 1


text: public

text: bioanode metagenome

text: bioanode metagenome

text: pre-culture 2.a


text: public

text: bioanode metagenome

text: bioanode metagenome

text: pre-culture 2.b


text: public

text: electrochemical batch reactor biofilm mcrA

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 1 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 2 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 3 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-CA 4 (pre2.b)


text: public

text: electrochemical batch reactor biofilm OCP control mcrA

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 1 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 2 (pre2.b)


text: public

text: bioanode metagenome

text: bioanode metagenome

text: EBR-OCP 3 (pre2.b)


text: The James Hutton Institute

text: public

tag: Namespace:The James Hutton Institute

text: mutant

characteristic: biological status

characteristic: collection date

text: domesticated barley

characteristic: common name

text: Hordeum vulgare cv. Bowman M2 EMS induced mutations leaf sample

text: Ethyl methanesulfonate (EMS) treatment

characteristic: genetic modification

text: Bowman

characteristic: genotype

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: growth chamber

characteristic: growth facility

text: seedstore

characteristic: isolation and growth condition

text: Hordeum vulgare subsp. vulgare

text: Barley

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

characteristic: plant structure

text: diploid

characteristic: ploidy

text: Hordeum vulgare subsp. vulgare

text: cultivar

characteristic: subspecific genetic lineage rank


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The James Hutton Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: mutant

text: domesticated barley

text: Hordeum vulgare cv. BW230 M2 EMS induced mutations leaf sample

characteristic: description

text: Ethyl methanesulfonate (EMS) treatment

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: growth chamber

text: seedstore

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Barley

text: soil

text: diploid

text: Hordeum vulgare subsp. vulgare

text: cultivar

characteristic: title


text: The James Hutton Institute

text: public

characteristic: Submitter Id

text: mutant

characteristic: biological status

characteristic: collection date

text: domesticated barley

characteristic: common name

text: Hordeum vulgare cv. Bowman M3 EMS induced mutations leaf sample

text: Ethyl methanesulfonate (EMS) treatment

characteristic: genetic modification

text: Bowman

characteristic: genotype

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: growth chamber

characteristic: growth facility

text: seedstore

characteristic: isolation and growth condition

text: Hordeum vulgare subsp. vulgare

text: Barley

characteristic: organism common name

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: diploid

text: Hordeum vulgare subsp. vulgare

text: cultivar


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Interfactulty Bioinformatics Unit, Department of Biology, University of Bern

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pig

text: Protocols: A total of 30 male and female Large White domestic pigs, 10 to 11 weeks-old and with a body weight of approximately 20-25 kg were obtained from the IVI specific pathogen-free (SPF) breeding facility or a local farm. The SPF and farm pigs were moved to the BSL-3Ag containment facilities and randomly assigned to separate groups of six or three animals, for virus- or mock-infection respectively. During the trial, the animals were fed a commercial pig pellet diet (Granovit AG, Kaiseraugst, Switzerland) with hay supplementation and water ad libitum. After 5 days of acclimatization in the BSL-3Ag containment, the pigs were infected by intramuscular injection of 2 ml DMEM containing ⁓3 to 6x102 TCID50/ml of either the highly virulent Armenia 2008, or the naturally attenuated Estonia 2014 strain. Mock-infected animals received 2 ml of DMEM. Blood samples were collected 1-3 days prior to infection and on 1, 2, 4, 5, 7, 9, 11, 14, 21 and 26 days post-infection, or until discontinuation criteria were reached. Whole blood RNA extraction whole blood kit Lexogen QuantSeq FWD

characteristic: description

text: piglet

characteristic: developmental stage

text: farm

characteristic: growth condition

characteristic: individual

text: African swine fever virus Armenia 2008

characteristic: infect

text: not applicable

characteristic: isolate

text: Sus scrofa

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

characteristic: sex

text: Large White

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Interfactulty Bioinformatics Unit, Department of Biology, University of Bern

text: public

characteristic: Submitter Id

text: ArrayExpress

text: pig

characteristic: common name

text: piglet

text: farm

text: African swine fever virus Armenia 2008

text: not applicable

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: Large White

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: piglet

text: farm

text: African swine fever virus Armenia 2008

text: not applicable

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: Large White


text: public

text: ArrayExpress

text: pig

text: piglet

characteristic: developmental stage

text: farm

characteristic: growth condition

characteristic: individual

text: African swine fever virus Estonia 2014

characteristic: infect

text: not applicable

characteristic: isolate

text: Sus scrofa

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: female

characteristic: sex

text: Large White

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: pig

characteristic: common name

text: piglet

text: farm

text: not applicable

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

text: Sus scrofa

text: male

text: Large White

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: piglet

text: farm

text: not applicable

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: Large White


text: public

text: ArrayExpress

text: pig

text: piglet

characteristic: developmental stage

text: specific pathogen-free facility

characteristic: growth condition

characteristic: individual

text: African swine fever virus Armenia 2008

characteristic: infect

text: not applicable

characteristic: isolate

text: Sus scrofa

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

characteristic: sex

text: Large White

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: pig

characteristic: common name

text: piglet

text: specific pathogen-free facility

text: African swine fever virus Armenia 2008

text: not applicable

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: Large White

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: piglet

text: specific pathogen-free facility

text: African swine fever virus Estonia 2014

text: not applicable

text: Sus scrofa

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: Large White


text: public

text: ArrayExpress

text: pig

text: piglet

characteristic: developmental stage

text: specific pathogen-free facility

characteristic: growth condition

characteristic: individual

characteristic: infect

text: not applicable

characteristic: isolate

text: Sus scrofa

text: Sus scrofa

text: blood

characteristic: organism part

text: Sus scrofa

text: male

characteristic: sex

text: Large White

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

text: ArrayExpress

text: pig

characteristic: common name

text: piglet

text: specific pathogen-free facility

text: not applicable

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: blood

text: Sus scrofa

text: female

text: Large White

characteristic: title


text: UNIVERSITY OF LIVERPOOL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF LIVERPOOL

text: Colony isolated on tryptic soy agar from a general purpose cleaner, on the week of contamination

characteristic: description

text: No

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: Pseudomonas oleovorans

text: Pseudomonas oleovorans

text: C1 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Colony isolated on tryptic soy agar from a general purpose cleaner, two weeks post-contamination

text: No

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: Pseudomonas oleovorans

text: Pseudomonas oleovorans

text: C1 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Colony isolated on Reasoner's 2A agar from a general purpose cleaner, on the week of contamination

text: No

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: Pseudomonas oleovorans

text: Pseudomonas oleovorans

text: C2 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Colony isolated on Reasoner's 2A agar from a general purpose cleaner, two weeks post-contamination

text: No

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: Pseudomonas oleovorans

text: Pseudomonas oleovorans

text: C2 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-1, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -1 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-1, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -1 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-2, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -2 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-2, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -2 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-3, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -3 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-3, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -3 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-4, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -4 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-4, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -4 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-5, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -5 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-5, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -5 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-6, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -6 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-6, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -6 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-7, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -7 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a contaminated general-purpose cleaner from a historic contamination incident, diluted to 10^-7, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC -7 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-1, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -1 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-1, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -1 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-2, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -2 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-2, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -2 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-3, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -3 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-3, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -3 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-4, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -4 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-4, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -4 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-5, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -5 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-5, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -5 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-6, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -6 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-6, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -6 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-7, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -7 W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from a uncontaminated (control) general-purpose cleaner, diluted to 10^-7, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK -7 W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from undiluted, uncontaminated (control) general-purpose cleaner, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK NEAT W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from undiluted, uncontaminated (control) general-purpose cleaner, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC BLANK NEAT W3


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from undiluted, contaminated general-purpose cleaner from a historic contamination incident, on the week of contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC NEAT W1


text: UNIVERSITY OF LIVERPOOL

text: public

text: Direct DNA extraction from undiluted, contaminated general-purpose cleaner from a historic contamination incident, two weeks post-contamination

text: Yes

text: Edward Cunningham-Oakes

text: general-purpose cleaner

text: metagenome

text: metagenome

text: GPC NEAT W3


text: University of Sao Paulo

text: public

tag: Namespace:University of Sao Paulo

text: Dr. Aline Cesar's team

text: Large White immunocastrated;" ~130 kg of body weight

text: Brazil

characteristic: geographic location (country and/or sea)

text: "Molecular Biology and Micotoxin Laboratory, ESALQ/USP, Piracicaba, SP, Brazil"

text: Sus scrofa scrofa

text: Sus scrofa scrofa

text: male

text: skeletal muscle


text: ROTHAMSTED RESEARCH

text: public

tag: Namespace:ROTHAMSTED RESEARCH

characteristic: collection date

text: soybean looper

text: Low coverage HiFi genome assembly for the Chrysodeixis includens genome. Unknown sex individual taken for nanopore, 10X Illumina, and multi-individual for Hi-C sequencing (Arima kit). One individual HiFi using 1 smrt cell but low coverage. 1988 from South Carolina USA. More recent collections from Athens, Georgia, USA.

text: USA

text: Chrysodeixis includens

text: Chrysodeixis includens

text: Low coverage HiFi genome assembly for the Chrysodeixis includens genome from the Pest Genomics Initiative.


text: Glasgow Polyomics

text: public

tag: Namespace:Glasgow Polyomics

text: CrispR Line

text: Trypanosoma brucei

text: Trypanosoma brucei

text: CRISPR editing of Topoisomerase II alpha in Trypanosoma brucei


text: Glasgow Polyomics

text: public

text: Wildtype

text: Trypanosoma brucei

text: Trypanosoma brucei


NI

text: Glasgow Polyomics

text: public

text: NI

text: CrispR Line

text: Trypanosoma brucei

text: Trypanosoma brucei


NII

text: Glasgow Polyomics

text: public

text: NII

text: CrispR Line

text: Trypanosoma brucei

text: Trypanosoma brucei


WT

text: Glasgow Polyomics

text: public

text: WT

text: Wildtype

text: Trypanosoma brucei

text: Trypanosoma brucei


text: INRA-CMSE

text: public

text: crops

characteristic: current land use

text: Repeated application of organic mixed with inorganic materials in agriculture improves soil microbial quality and by extension crop productivity. While soil microbial quality at the end point following several years of a repeated fertilization diets have received considerable attention, the dynamic of this community throughout years had never been assessed. Here, we use a high-throughput sequencing approach targeting 16S ribosomal RNA gene to investigate throughout 6 years of repeated fertilizers (organic and inorganic) application the prokaryotic community evolution. Soils were sampled from a field experiment: La Mare (Reunion Island, France), where different mixed organic-inorganic fertilization diets characterized by more or less stable organic matter were applied regularly since 6 years. Each year, approximately more than 6 months after the last fertilization, soil samples were carried out. Our results highlighted that soil microbial community was lastingly modified after 6 years of repeated fertilization diets. These changes depended both on the nature of organic input and fertilization practices (frequencies and quantity applied).

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CATACTCTAC(forward) CACGTAGATC (reverse)

characteristic: multiplex identifiers

text: Microbial DNA was extracted from 1 g (dry weight) of soil using a single procedure standardized by the GenoSol platform (INRAE, Dijon, France, https://www2.dijon.inrae.fr/plateforme_genosol/) . Since a highly positive linear relationship has been shown between soil DNA recovery and C-biomass measurement , DNA concentrations of crude extracts were determined by electrophoresis in 1% agarose gel stained with ethidium bromide using a calf thymus DNA standard curve, and used as estimates of microbial biomass . After quantification, 100 µl of crude DNA extract were separated from the residual impurities, particularly humic substances, by using only the purification steps of Nucleospin Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Purified DNA concentrations were finally measured using the Quantifluor (Promega, Lyon, France) staining kit, according to the manufacturer’s instructions.

characteristic: nucleic acid extraction

text: soil metagenome

text: 5-CAGCMGCYGCNGTAANAC-3 (forward) 5-CCGYCAATTCMTTTRAGT-3 (reverse)

characteristic: pcr primers

text: ReunPro

characteristic: project name

unit: g

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene

text: Microbial diversity dynamic of tropical soil during 6 years of repeated organic and inorganic fertilization


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CGCTATCTAT(forward) CGCTATCTAT (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CACGTAGATC(forward) CACGCTACGT (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CGACGTGACT(forward) CGTCGATCTC (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CATACTCTAC(forward) CGCGACATCT (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CGCGACATCT(forward) CGAGCTCATG (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CATACTCTAC(forward) CACGCTACGT (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CACGTAGATC(forward) CGAGCTCATG (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGTACAGTCA(forward) CGTCGATCTC (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATAGTAGTG(forward) CACGCTACGT (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGTCGATCTC(forward) CGCGACATCT (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CGACGTGACT(forward) CATAGTAGTG (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CATAGTAGTG(forward) CGAGCTCATG (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

text: Repeated application of organic mixed with inorganic materials in agriculture improves soil microbial quality and by extension crop productivity. While soil microbial quality at the end point following several years of a repeated fertilization diets have received considerable attention, the dynamic of this community throughout years had never been assessed. Here, we use a high-throughput sequencing approach targeting 16S ribosomal RNA gene to investigate throughout 6 years of repeated fertilizers (organic and inorganic) application the prokaryotic community evolution. Soils were sampled from a field experiment: La Mare (Reunion Island, France), where different mixed organic-inorganic fertilization diets characterized by more or less stable organic matter were applied regularly since 6 years. Each year, approximately more than 6 months after the last fertilization, soil samples were carried out. Our results highlighted that soil microbial community was lastingly modified after 6 years of repeated fertilization diets. These changes depended both on the nature of organic input and fertilization practices (frequencies and quantity applied).

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATACTCTAC(forward) CGTCGATCTC (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGTCGATCTC(forward) CGTCGATCTC (reverse)

characteristic: multiplex identifiers

text: Microbial DNA was extracted from 1 g (dry weight) of soil using a single procedure standardized by the GenoSol platform (INRAE, Dijon, France, https://www2.dijon.inrae.fr/plateforme_genosol/) . Since a highly positive linear relationship has been shown between soil DNA recovery and C-biomass measurement , DNA concentrations of crude extracts were determined by electrophoresis in 1% agarose gel stained with ethidium bromide using a calf thymus DNA standard curve, and used as estimates of microbial biomass . After quantification, 100 µl of crude DNA extract were separated from the residual impurities, particularly humic substances, by using only the purification steps of Nucleospin Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Purified DNA concentrations were finally measured using the Quantifluor (Promega, Lyon, France) staining kit, according to the manufacturer’s instructions.

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CACGTAGATC(forward) CACGTAGATC (reverse)

text: soil metagenome

characteristic: organism

text: 5-CAGCMGCYGCNGTAANAC-3 (forward) 5-CCGYCAATTCMTTTRAGT-3 (reverse)

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

text: Microbial diversity dynamic of tropical soil during 6 years of repeated organic and inorganic fertilization

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGACGTGACT(forward) CACGCTACGT (reverse)

characteristic: multiplex identifiers

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text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

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text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

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text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATCGAGCAG(forward) CATATCTCGT (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

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text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

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characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

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text: soil metagenome

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characteristic: sequencing method

text: soil

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text: microbial feature

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text: France

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text: soil metagenome

characteristic: organism

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characteristic: title


text: INRA-CMSE

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characteristic: environment (feature)

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characteristic: geographic location (depth)

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text: microbial feature

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text: soil metagenome

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text: INRA-CMSE

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characteristic: environment (feature)

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characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

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characteristic: multiplex identifiers

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text: soil metagenome

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characteristic: sequencing method

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characteristic: title


text: INRA-CMSE

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characteristic: environment (feature)

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characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

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text: CATCGAGCAG(forward) CGACGTGACT (reverse)

characteristic: multiplex identifiers

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text: ReunPro

characteristic: project name

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text: soil metagenome

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characteristic: sequencing method

text: soil

characteristic: soil environmental package

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characteristic: current land use

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text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATACTCTAC(forward) CGACGTGACT (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: Repeated application of organic mixed with inorganic materials in agriculture improves soil microbial quality and by extension crop productivity. While soil microbial quality at the end point following several years of a repeated fertilization diets have received considerable attention, the dynamic of this community throughout years had never been assessed. Here, we use a high-throughput sequencing approach targeting 16S ribosomal RNA gene to investigate throughout 6 years of repeated fertilizers (organic and inorganic) application the prokaryotic community evolution. Soils were sampled from a field experiment: La Mare (Reunion Island, France), where different mixed organic-inorganic fertilization diets characterized by more or less stable organic matter were applied regularly since 6 years. Each year, approximately more than 6 months after the last fertilization, soil samples were carried out. Our results highlighted that soil microbial community was lastingly modified after 6 years of repeated fertilization diets. These changes depended both on the nature of organic input and fertilization practices (frequencies and quantity applied).

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CATACTCTAC(forward) CATACTCTAC (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CACGTAGATC(forward) CGTACAGTCA (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGTACAGTCA(forward) CGCGACATCT (reverse)

characteristic: multiplex identifiers

text: Microbial DNA was extracted from 1 g (dry weight) of soil using a single procedure standardized by the GenoSol platform (INRAE, Dijon, France, https://www2.dijon.inrae.fr/plateforme_genosol/) . Since a highly positive linear relationship has been shown between soil DNA recovery and C-biomass measurement , DNA concentrations of crude extracts were determined by electrophoresis in 1% agarose gel stained with ethidium bromide using a calf thymus DNA standard curve, and used as estimates of microbial biomass . After quantification, 100 µl of crude DNA extract were separated from the residual impurities, particularly humic substances, by using only the purification steps of Nucleospin Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Purified DNA concentrations were finally measured using the Quantifluor (Promega, Lyon, France) staining kit, according to the manufacturer’s instructions.

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATATCTCGT(forward) CACGCTACGT (reverse)

text: soil metagenome

characteristic: organism

text: 5-CAGCMGCYGCNGTAANAC-3 (forward) 5-CCGYCAATTCMTTTRAGT-3 (reverse)

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGAGCTCATG(forward) CATCGAGCAG (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene

text: Microbial diversity dynamic of tropical soil during 6 years of repeated organic and inorganic fertilization


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CGCGACATCT(forward) CATCGAGCAG (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGAGCTCATG(forward) CATACTCTAC (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATCGAGCAG(forward) CACGTAGATC (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: INRA-CMSE

text: public

text: crops

text: agricultural soil

text: microbial feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: France

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Réunion

characteristic: geographic location (region and locality)

characteristic: investigation type

text: CGAGCTCATG(forward) CGTACAGTCA (reverse)

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

text: ReunPro

characteristic: project name

characteristic: sample volume or weight for dna extraction

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: crops

characteristic: current land use

characteristic: description

text: agricultural soil

characteristic: environment (biome)

text: microbial feature

text: soil

text: France

characteristic: geographic location (country and/or sea)

text: Réunion

text: CATATCTCGT(forward) CATACTCTAC (reverse)

text: soil metagenome

characteristic: organism

text: ReunPro

text: soil metagenome

text: Illumina

text: soil

characteristic: title