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characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-ETHZ-37841998/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-37870196/2022

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-ETHZ-37809258/2022


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Labor team w AG; Blumeneggstrasse 55, 9403 Goldach

text: Rebekka Pohl, Monika Bucher, Andreas Lindauer, Andreas Grutsch, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: ZH

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Viollier AG; Hagmattstrasse 14, 4123 Allschwil

text: Switzerland

text: BL

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BL-ETHZ-37878853/2022

characteristic: organism

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BL

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BL-ETHZ-37846778/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BL

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BL-ETHZ-37880206/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: AG

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/AG-ETHZ-37862749/2022

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: TI

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/TI-ETHZ-37875430/2022


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Switzerland

text: ZH

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-ETHZ-37893846/2022

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-37886225/2022

characteristic: organism

text: active surveillance in response to outbreak

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: AG

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/AG-ETHZ-37870998/2022

text: active surveillance not initiated by an outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: BE

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-ETHZ-37869109/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: TG

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-ETHZ-37873627/2022


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Labor team w AG; Blumeneggstrasse 55, 9403 Goldach

text: Switzerland

text: AG

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-37864023/2022

characteristic: organism

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Viollier AG; Hagmattstrasse 14, 4123 Allschwil

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: ZH

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-ETHZ-37708121/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: BE

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-ETHZ-37847871/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-ETHZ-37867710/2022

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: SO

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/SO-ETHZ-37858539/2022


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Switzerland

text: BS

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BS-ETHZ-37886658/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: ZG

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZG-ETHZ-37860994/2022

characteristic: organism

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-ETHZ-37887603/2022

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

text: Switzerland

text: VD

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-ETHZ-37866689/2022

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: AG

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/AG-ETHZ-37919222/2022

characteristic: organism

text: active surveillance not initiated by an outbreak

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BL

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BL-ETHZ-37881324/2022


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Switzerland

text: BE

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-ETHZ-37875982/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BE-ETHZ-37861627/2022

characteristic: organism

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: Viollier AG; Hagmattstrasse 14, 4123 Allschwil

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: SO

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/SO-ETHZ-37892649/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: BL

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BL-ETHZ-37871607/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:ROSLIN INSTITUTE, UNIVERSITY OF EDINBURGH

characteristic: Submitter Id

text: Whippet

characteristic: breed

text: dog

characteristic: common name

text: Polyarthropathy - immune-mediated, Masticatory myositis, Enteropathy

characteristic: description

text: Canis lupus familiaris

characteristic: organism

text: Canis lupus familiaris

text: F

characteristic: sex

text: WGS of dogs and cats with suspected heritable conditions

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ROSLIN INSTITUTE, UNIVERSITY OF EDINBURGH

characteristic: INSDC last update

text: public

text: Standard Poodle

text: dog

text: lipotamous mass in prescapula

text: Canis lupus familiaris

text: Canis lupus familiaris

text: F


text: public

text: Flatcoated retriever

text: dog

text: Aspergillosis

text: Canis lupus familiaris

text: Canis lupus familiaris

text: F


text: public

text: Collie

text: dog

text: Canis lupus familiaris

text: Canis lupus familiaris

text: M


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

text: public

text: SIB

characteristic: broker name

text: Labor team w AG; Blumeneggstrasse 55, 9403 Goldach

characteristic: collecting institution

characteristic: collection date

text: Rebekka Pohl, Monika Bucher, Andreas Lindauer, Andreas Grutsch, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

characteristic: isolate

characteristic: sample capture status


E-MTAB-11950:Riboseq-Col-0 biol rep 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11950:Riboseq-Col-0 biol rep 1

tag: Namespace:Botanical Institute of the Christian-Albrechts-University of Kiel Genetics and molecularbiology in plants

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

characteristic: common name

text: Protocols: Flowers were harvested simultaneously for all genotypes, snap-frozen in liquid nitrogen and stored at -80°C until use. The plants were grown in the Greenhouse under long day conditions. Total ribosome footprints were prepared from a mix of flower buds and open flowers. About 2.5 g of flowers were ground in liquid nitrogen and resuspended in 15 ml of ribosome extraction buffer (0.2 M sucrose, 0.2 M KCl, 40 mM Tris-acetate, pH 8, 10 mM MgCl2, 10 mM b-mercaptoethanol, 2% polyoxyethylene 10 tridecyl ether, 1% Triton X-100, 100 mg/ml chloramphenicol and 100 mg/ml cycloheximide). The obtained solution was centrifuged twice for 10 min at 15 000 g at 4◦C to remove the cell debris and the supernatant was transferred to a new tube. To estimate the RNA concentration contained in the supernatant, total RNA was extracted from a 0.5 ml aliquot using the TRI Reagent® (Sigma- Aldrich) following the manufacturer’s instructions. The supernatant was then digested with 10 U/OD260nm/ml of RNAse I (Ambion), and incubated 1 h at 25◦C to degrade unprotected RNA. The solution was then layered on an equal volume of a sucrose cushion (1 M Sucrose, 0.1 M KCl, 40 mM Tris-acetate, pH 8, 15 mM MgCl2, 5 mM b-mercaptoethanol, 100 mg/ml chloramphenicol and 100 mg/ml cycloheximide) and centrifuged for 3 h at 55 000 g at 4◦C in a TFT 65-13 rotor (Kontron). The obtained pellet was resuspended in 0.6 ml of RNA extraction buffer I (0.1 M EGTA pH 8, 0.1 M NaCl, 1% sodium dodecyl sulphate (SDS), 10 mM Tris–HCl pH 8 and 1 mM ethylenediaminetetraacetic acid (EDTA)), and RNAs were extracted with the TRI Reagent® (Sigma-Aldrich) following the manufacturer’s recommendations. Ribosomal footprints were further purified on 17% (v/v) acrylamide- bisacrylamide (19:1) gel made with 7 M urea and 1 × Tris- acetate-EDTA (TAE) buffer. For this, 600 mg of RNAs were electrophoresed in two separated 15 cm-wide wells. The gel was pre-run for 1 h at 50◦C and then run for 15–16 h at 90 V at the same temperature. A mix comprising 100 ng of 27- mer and 33-mer RNA oligonucleotides was loaded on both gel extremities and used as size marker. After staining for 30 min in 1× SYBR Gold (Life Technologies) diluted in 1× TAE, RNA contained in the 27 and 33 nt regions were separately excised from the gel. The gels slices were stored at least 2 h at -20◦C and then physically disrupted by centrifugation through a needle hole made in a 0.5 ml microcentrifuge tube nested in an outer 1.5 ml collection microtube. The tube assembly was centrifuged at maximum speed for 5 min. RNAs were eluted overnight from gel fragments by passive diffusion in 5 ml of RNA extraction buffer II (10 mM Tris–HCl pH 8, 1 mM EDTA, 0.1 M NaCl, and 0.2%SDS) at 4◦C on a rotating wheel. The recovered RNAs were then isolated with the TRI Reagent® (Sigma-Aldrich) following the manufacturer’s instructions, resuspended in 30 ml of RNAse-free water and stored at −80◦C until use. Ribosome footprints were depleted from rRNA with the RiboMinusTM Plant Kit for RNA-Seq (invitrogen), following the manufacturer’s instructions. The sequencing libraries of ribosomal footprints of two biological repeats per genotype were prepared using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific) for Ribo-Seq following the manufacturer's instructions.

characteristic: description

characteristic: ecotype

text: wild type genotype

characteristic: genotype

text: not applicable

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: flower

characteristic: organism part

text: Arabidopsis thaliana

text: Riboseq-Col-0 biol rep 1

characteristic: title


E-MTAB-11950:Riboseq-Col-0 biol rep 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Botanical Institute of the Christian-Albrechts-University of Kiel Genetics and molecularbiology in plants

characteristic: INSDC last update

text: public

text: E-MTAB-11950:Riboseq-Col-0 biol rep 2

text: ArrayExpress

characteristic: broker name

text: thale cress

text: wild type genotype

text: not applicable

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

text: Arabidopsis thaliana

text: Riboseq-Col-0 biol rep 2


E-MTAB-11950:Riboseq-dweorg1 biol rep 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11950:Riboseq-dweorg1 biol rep 1

text: ArrayExpress

text: thale cress

text: DWEORG1 mutant

text: not applicable

characteristic: isolate

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

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text: Riboseq-dweorg1 biol rep 1


E-MTAB-11950:Riboseq-dweorg1 biol rep 2

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text: E-MTAB-11950:Riboseq-dweorg1 biol rep 2

text: ArrayExpress

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text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: flower

text: Arabidopsis thaliana

text: Riboseq-dweorg1 biol rep 2


text: Control

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tag: Namespace:Clinical Microbiomics A/S

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characteristic: geographic location (country and/or sea)

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characteristic: ENA-CHECKLIST

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