characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Fusobacterium sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Fusobacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606222_bin.16_metaWRAP_v1.3_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1606222 of study ERP017012.

text: Feces

text: China

unit: DD

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: [Clostridium] symbiosum

text: A comprehensive sequencing evaluation of the fecal microbial composition and functional perturbation in the diarrhea condition of suckling piglets.

text: [Clostridium] symbiosum

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__[Clostridium] symbiosum

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606222_bin.2_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606222_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Fusobacterium mortiferum

text: Fusobacterium mortiferum

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium;s__Fusobacterium mortiferum

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606222_bin.5_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Bacteroides xylanisolvens

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Bacteroides xylanisolvens

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides xylanisolvens

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606222_bin.6_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Fusobacterium varium

text: Fusobacterium varium

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium;s__Fusobacterium varium

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606222_bin.8_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1606358 of study ERP017012.

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Anaeromassilibacillus sp.

text: uncultured Anaeromassilibacillus sp.

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Anaeromassilibacillus;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.10_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Solobacterium sp.

characteristic: organism

text: A comprehensive sequencing evaluation of the fecal microbial composition and functional perturbation in the diarrhea condition of suckling piglets.

characteristic: project name

characteristic: sample derived from

text: uncultured Solobacterium sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Solobacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Veillonella caviae

text: Veillonella caviae

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella;s__Veillonella caviae

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.13_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Alistipes sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Alistipes sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.14_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Faecalicatena sp.

text: uncultured Faecalicatena sp.

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Faecalicatena;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.15_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Streptococcus pasteurianus

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Streptococcus pasteurianus

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus pasteurianus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.16_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1606358 of study ERP017012.

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Sutterellaceae bacterium

text: uncultured Sutterellaceae bacterium

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Sutterellaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.17_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Collinsella sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Collinsella sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__Coriobacteriaceae;g__Collinsella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.18_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: [Clostridium] scindens

text: A comprehensive sequencing evaluation of the fecal microbial composition and functional perturbation in the diarrhea condition of suckling piglets.

text: [Clostridium] scindens

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__[Clostridium] scindens

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.2_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Desulfovibrio piger

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Desulfovibrio piger

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Deltaproteobacteria;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Desulfovibrio;s__Desulfovibrio piger

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.21_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Bacteroides fragilis

text: Bacteroides fragilis

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides fragilis

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.22_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Parabacteroides distasonis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Parabacteroides distasonis

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides;s__Parabacteroides distasonis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.23_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Bacteroides sp.

text: uncultured Bacteroides sp.

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides vulgatus

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.24_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1606358 of study ERP017012.

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.25_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Clostridium sp.

text: uncultured Clostridium sp.

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.3_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Alistipes senegalensis

characteristic: organism

text: A comprehensive sequencing evaluation of the fecal microbial composition and functional perturbation in the diarrhea condition of suckling piglets.

characteristic: project name

characteristic: sample derived from

text: Alistipes senegalensis

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__Alistipes senegalensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.4_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Clostridia bacterium

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.5_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus reuteri

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.6_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Eisenbergiella massiliensis

text: Eisenbergiella massiliensis

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Eisenbergiella;s__Eisenbergiella massiliensis

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.7_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR1606358_bin.8_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1606358 of study ERP017012.

text: Feces

text: China

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Escherichia coli

text: Escherichia coli

text: Illumina HiSeq 2500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli

text: multi-marker approach

text: Metagenome-assembled genome: ERR1606358_bin.9_metaWRAP_v1.3_MAG

characteristic: title


Controle negativo 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

text: public

text: Controle negativo 1

tag: Namespace:VALE INSTITUTE OF TECHNOLOGY

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

text: Universidade Federal do Pará

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

text: Controle negativo 1

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status


Controle negativo 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Controle negativo 2

characteristic: Submitter Id

text: ITV Vale Institute of Technology

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: not provided

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: Controle negativo 2

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

text: public

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: BELÉM

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: male

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: CASTANHAL

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: AURORA DO PARA

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: SAO JOAO DE PIRABAS

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: male

text: not provided


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: BUJARU

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: SAO JOAO DE PIRABAS

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: REDENCAO

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: female

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: ANANINDEUA

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: ALTAMIRA

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: ANANINDEUA

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: female

text: not provided


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: REDENCAO

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: BELEM

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: GARRAFAO DO NORTE

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: female

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: SAO DOMINGOS DO CAPIM

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: SAO JOAO DO ARAGUAIA

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: TAILANDIA

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: female

text: not provided


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

text: Brazil

text: CAMETA

text: human

text: recovered

text: diseased

text: Homo sapiens

characteristic: host scientific name

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

text: Brazil

text: VIGIA

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ITV Vale Institute of Technology

text: not provided

text: Universidade Federal do Pará

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: AUGUSTO CORREA

characteristic: geographic location (region and locality)

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

text: male

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

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text: public

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text: not provided

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text: Brazil

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text: Brazil

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text: BELÉM

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text: Brazil

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text: MOSQUEIRO

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text: not provided

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text: Brazil

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text: ANANINDEUA

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text: Homo sapiens

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characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

text: not provided

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: BELÉM

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

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text: not provided

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characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

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text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: BELÉM/MOSQUEIRO

characteristic: geographic location (region and locality)

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: External Id

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characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: ANANINDEUA

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text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

text: not provided


characteristic: ENA-CHECKLIST

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text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

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text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

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text: PONTA DE PEDRAS

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text: diseased

text: Homo sapiens

text: female

characteristic: host sex

characteristic: host subject id

text: not provided

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characteristic: organism

characteristic: sample capture status


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

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text: public

characteristic: INSDC status

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text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: BENFICA

characteristic: geographic location (region and locality)

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

text: Brazil

text: BENEVIDES

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text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

text: not provided


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text: public

characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: JOANES

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

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characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: OUTEIRO

characteristic: geographic location (region and locality)

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: PONTA DE PEDRAS

characteristic: host age

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

text: Brazil

text: COQUEIRO

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: BELÉM

characteristic: geographic location (region and locality)

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

text: not provided

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

text: Brazil

text: BELÉM

characteristic: host age

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

text: not provided


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: human

text: recovered

text: diseased

text: Homo sapiens

text: male

characteristic: host sex

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: ITV Vale Institute of Technology

characteristic: collecting institution

text: Universidade Federal do Pará

characteristic: collector name

characteristic: description

text: Brazil

characteristic: geographic location (country and/or sea)

text: BELÉM

characteristic: geographic location (region and locality)

text: human

text: recovered

text: diseased

text: Homo sapiens

text: female

text: not provided

characteristic: title


characteristic: External Id

text: VALE INSTITUTE OF TECHNOLOGY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ITV Vale Institute of Technology

characteristic: collection date

text: Universidade Federal do Pará

text: Brazil

text: CALI

characteristic: host age

text: human

characteristic: host common name

text: recovered

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: male

text: not provided


E-MTAB-11991:Sample 2_lck_GO+BA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: E-MTAB-11991:Sample 2_lck_GO+BA

tag: Namespace:Institute of Environmental Medicine, Karolinska Institutet

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: zebrafish

characteristic: common name

text: Protocols: The generation of germ-free Tg(lck:GFP) zebrafish followed previously established protocols [1]. In brief, 2 hpf embryos were transferred to petri dishes with sterile E3 medium, supplemented with ampicillin (100 μg/mL), kanamycin (5 μg/mL) and amphotericin B (250 ng/mL) and incubated at 28°C. At 50% epiboly up to shield stage (6 hpf), the embryos were surface disinfected with 0.1% of polyvinylpyrroidone-iodine (PVP-I) for exactly 2 min, followed by 0.003% sterile bleach immersion for 18 min. The embryos were then rinsed with sterile E3 medium, transferred to flasks and incubated at 28°C. The viability was monitored, and sterile medium was refreshed daily. At day 4, the hatched embryos were used for the sterility validation. A day 5, germ-free Tg(lck:GFP) zebrafish were exposed to the combination of GO (30 µg/mL) and butyrate (2.5 mM) for 24 h. GO and BA were pre-incubated for 1 h prior to the exposure. Fifty larvae were used as one replicate, four replicates, i.e. two hundred larvae in total, were used for each condition. After the exposure, zebrafish larvae were dissociated for single cell suspension following the published protocol [2]. Briefly, zebrafish larvae were euthanized with 0.01% of tricaine for 5 min, collected in 1.5 mL tube, and washed with PBS for three times. The dissociation was initiated by adding 500 μL of pre-warmed enzyme mix containing 460 μL of 0.25% trypsin-EDTA (Gibco) and 40 μL of collagenase (Sigma-Aldrich) at the concentration of 100 mg/mL, followed by mechanical dissociation using P1000 and then P200 pipette tips in heat-block at 30 °C until the tissue was no longer visible (about 10 min). The dissociation was then stopped by adding 800 μL DMEM (Corning Cat# 10-013-CV) with 10% FBS (Fisher Scientific). The cell pellets were collected by centrifuging at 1000 rpm for 5 min at RT, followed by washing with PBS. The cells were next resuspended in 0.5 mL DMEM with 10% FBS. Four replicates from each condition were pooled together at this step and filtered through 40 μm nylon mesh, with an additional washing using DMEM with 10% FBS. The cell suspension was then stained with a fluorescent DNA dye DRAQ7 to exclude non-viable cells (Invitrogen Cat# D15106) at the dose of 3 μM for 10 min at room temperature before the fluorescence-activated cell sorting (BD FACSAria III, BD Biosciences, NJ, USA). The sorting was performed using a 100 microns nozzle with the flow rate of 2 and temperature control at 4 °C and gated based on forward scatter and DRAQ7. The DRAQ7-lck+ cells of each sample were FACS sorted into the tubes containing DMEM with 10% FBS and placed on ice immediately after the sorting. The cells were then proceeded with the single-cell RNA sequencing analysis. 1. Pham LN, Kanther M, Semova I & Rawls JF. Methods for generating and colonizing gnotobiotic zebrafish. Nat. Protoc. 3, 1862-1875 (2008). 2. Bresciani E, Broadbridge E, Liu PP An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae, MethodsX. 5, 1287-1290 (2018). Single-cell RNA sequencing (scRNA-seq) was performed by Eukaryotic Single Cell Genomics Facility at SciLifeLab, Stockholm. The single cells collected from FACS sorting were loaded on a 10x GemCode Single-Cell Instrument (10x Genomics, Pleasanton, USA) to generate single-cell gel beads in emulsion (GEMs) using the Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 (10x Genomics) following the manufacture’s protocol. Briefly, GEMs were generated by combining barcoded Single Cell 3’ v3.1 Gel Beads, a Master Mix containing cells, and Partitioning Oil onto Chromium Next GEM Chip G. Following GEMs generation, the Gel Bead was dissolved, primers containing an Illumina TruSeq Read 1(read 1 sequencing primer), 16 nt 10x Barcode, 12 nt unique molecular identifier (UMI) and 30 nt poly(dT) sequence were released, and the co-partitioned cells were lysed. The nucleic acid library construction was performed using the Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 (10x Genomics, 16 rxns PN-1000268) following the manufacture’s protocol. After GEMs generation, primers were mixed with the cell lysate and a Master Mix containing reverse transcription (RT) reagents to produce barcoded, full-length cDNA from polyadenylated mRNA. Silane magnetic beads were used to purify the first-strand cDNA from the post GEM-RT reaction mixture, which included leftover biochemical reagents and primers. Barcoded, full-length cDNA was amplified via PCR to generate sufficient mass for library construction. Enzymatic fragmentation and size selection were used to optimize the cDNA amplicon size. P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 (read 2 primer sequence) were added via End Repair, A-tailing, Adaptor Ligation, and PCR. The final libraries containing the P5 and P7 primers used in Illumina amplification were prepared for an estimated 5000 nuclei per sample.

text: larva

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Danio rerio

characteristic: organism

text: Danio rerio

text: whole organism

characteristic: organism part

text: Danio rerio

text: Tg(lck:GFP)

characteristic: strain

text: Sample 2_lck_GO+BA