characteristic: ENA-LAST-UPDATE

text: public

text: DNA

text: house mouse

text: DNA sample isolated from mouse hippocampus (Endurance condition)

text: Mus musculus

text: Mus musculus

text: male

text: hippocampus


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Instituto de Investigacion Biosanitaria del Principado de Asturias (ISPA)

characteristic: Submitter Id

text: DNA

text: house mouse

characteristic: common name

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

text: male

characteristic: sex

text: hippocampus

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text: mixed

characteristic: ArrayExpress-SEX

text: Caenorhabditis imperialis

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Caenorhabditis imperialis

text: Mixed

characteristic: gender

text: Caenorhabditis imperialis

text: Caenorhabditis imperialis

characteristic: subject id


text: mixed

text: Caenorhabditis japonica

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Caenorhabditis japonica

text: Mixed

text: Caenorhabditis japonica

text: Caenorhabditis japonica


text: mixed

text: Caenorhabditis tribulationis

characteristic: External Id

text: Mixed

characteristic: GENDER

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Caenorhabditis tribulationis

text: Caenorhabditis tribulationis

text: Caenorhabditis tribulationis


text: mixed

text: Caenorhabditis sinica

text: Mixed

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Caenorhabditis sinica

text: Caenorhabditis sinica

text: Caenorhabditis sinica


text: mixed

text: Caenorhabditis macrosperma

text: Mixed

text: SC

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text: Caenorhabditis macrosperma

characteristic: common name

text: Caenorhabditis macrosperma

characteristic: organism

text: Caenorhabditis macrosperma


text: mixed

characteristic: ArrayExpress-Sex

text: Caenorhabditis monodelphis

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Caenorhabditis monodelphis

text: Caenorhabditis monodelphis

text: Caenorhabditis monodelphis

characteristic: strain

characteristic: title


text: MycoBank ID: 386581

characteristic: Description

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CANADIAN FOOD INSPECTION AGENCY

text: public

text: ena-SAMPLE-TAB-31-05-2021-17:49:20:599-621

tag: Namespace:CANADIAN FOOD INSPECTION AGENCY

text: mycelium

characteristic: cell type

text: Hudson Valley Research Laboratory, Cornell University

characteristic: collected by

characteristic: collection date

characteristic: culture collection

text: vegetative

characteristic: dev stage

text: USA

characteristic: geographic location (country and/or sea)

text: New York State, Hudson

characteristic: geographic location (region and locality)

text: Idared

characteristic: host cultivar

text: Malus domestica

characteristic: host scientific name

text: F. Khodadadi, P.L. Martin, V.P. Doyle, J.B. Gonzalez, S.G. Acimovic

characteristic: identified by

characteristic: isolate

text: Fruit lesion of Apple/Idared

characteristic: isolation source

text: Colletotrichum noveboracense

text: Colletotrichum noveboracense

text: Colletotrichum noveboracense isolate H1091258 - fungal plant pathogen causing Apple Bitter Rot


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Iceland

characteristic: Submitter Id

text: Arctic char

characteristic: common name

characteristic: description

text: 200 ts

text: PL

characteristic: ecotype

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

characteristic: organism

text: Salvelinus alpinus

text: Female

characteristic: sex


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Iceland

text: public

text: Arctic char

text: 200 ts

text: PL

text: Iceland

characteristic: geographic location (country and/or sea)

text: Lake Thingvallavatn

characteristic: geographic location (region and locality)

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Male

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

text: public

text: Arctic char

text: 200 ts

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text: Salvelinus alpinus

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text: Male


text: University of Iceland

characteristic: INSDC center name

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text: public

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text: Female


text: University of Iceland

characteristic: INSDC last update

text: public

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text: Salvelinus alpinus

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text: University of Iceland

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characteristic: SRA accession

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text: University of Iceland

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characteristic: common name

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text: Female


text: University of Iceland

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text: Arctic char

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characteristic: ecotype

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

characteristic: organism

text: Salvelinus alpinus

text: Male


characteristic: ENA-CHECKLIST

text: University of Iceland

text: public

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text: 150 ts

text: PI

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text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

text: University of Iceland

text: public

text: Arctic char

text: 150 ts

text: PL

text: Iceland

characteristic: geographic location (country and/or sea)

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Male


text: University of Iceland

text: public

text: Arctic char

text: 150 ts

text: SB

text: Iceland

text: Lake Thingvallavatn

characteristic: geographic location (region and locality)

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

text: public

text: Arctic char

text: 150 ts

text: PL

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

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text: Female


text: University of Iceland

characteristic: INSDC center name

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text: public

text: Arctic char

text: 150 ts

text: PI

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text: Lake Thingvallavatn

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text: Salvelinus alpinus

text: Male


text: University of Iceland

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Arctic char

text: 150 ts

text: SB

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Male


text: University of Iceland

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Arctic char

text: 150 ts

text: LB

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female


text: University of Iceland

text: public

text: Arctic char

characteristic: common name

characteristic: description

text: 100 ts

text: PL

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female


text: University of Iceland

text: public

text: Arctic char

text: 100 ts

text: PI

characteristic: ecotype

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

characteristic: organism

text: Salvelinus alpinus

text: Male


characteristic: ENA-CHECKLIST

text: University of Iceland

text: public

text: Arctic char

text: 100 ts

text: LB

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

text: University of Iceland

text: public

text: Arctic char

text: 100 ts

text: LB

text: Iceland

characteristic: geographic location (country and/or sea)

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Male


text: University of Iceland

text: public

text: Arctic char

text: 100 ts

text: SB

text: Iceland

text: Lake Thingvallavatn

characteristic: geographic location (region and locality)

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Male

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Iceland

text: public

text: Arctic char

text: 100 ts

text: PI

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female


text: University of Iceland

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Arctic char

text: 100 ts

text: SB

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus


text: University of Iceland

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Arctic char

text: 50 ts

text: PL

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus

text: Female


text: University of Iceland

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Arctic char

text: 50 ts

text: PI

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus


text: University of Iceland

text: public

text: Arctic char

characteristic: common name

characteristic: description

text: 50 ts

text: LB

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text: Lake Thingvallavatn

text: Salvelinus alpinus

text: Salvelinus alpinus


text: University of Iceland

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text: Arctic char

text: 50 ts

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characteristic: ecotype

text: Iceland

text: Lake Thingvallavatn

text: Salvelinus alpinus

characteristic: organism

text: Salvelinus alpinus


text: Escherichia coli

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

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text: Escherichia coli

text: Escherichia coli

text: Escherichia coli


text: Escherichia coli

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli


text: Streptococcus agalactiae

text: SC

text: public

text: Streptococcus agalactiae

text: Streptococcus agalactiae

text: Streptococcus agalactiae

characteristic: title


text: Staphylococcus aureus

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 263/Gest 3/70

characteristic: External Id

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Escherichia coli

text: SC

characteristic: INSDC center name

text: public

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli


text: Streptococcus uberis

text: SC

characteristic: INSDC first public

text: public

text: Streptococcus uberis

text: Streptococcus uberis

text: Streptococcus uberis


text: Staphylococcus sciuri

text: 9A h2 15

text: SC

characteristic: INSDC last update

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 9A h2 16

text: SC

text: public

characteristic: INSDC status

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 10A h2 23

text: SC

text: public

characteristic: SRA accession

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 23B h2 3

text: SC

text: public

characteristic: Submitter Id

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 24B h2 7

text: SC

text: public

text: Staphylococcus sciuri

characteristic: common name

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 25A h2 1

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 25A h2 2

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 27B h2 28

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 36C h2 11

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 38C h2 13

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 39B h2 6

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus aureus

text: 2A 8

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 2A 9

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 2B 7

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 3B 14

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 3C 11

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: 3C 12

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP145645/02 Haem-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus xylosus

text: CP146229/05 Tiny-1

text: SC

text: public

text: Staphylococcus xylosus

text: Staphylococcus xylosus

text: Staphylococcus xylosus


text: Staphylococcus aureus

text: CP145645/02 non-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staph pseudintermedius

text: CP146084/03 pred-1

text: SC

text: public

text: Staph pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staph pseudintermedius

text: SC

text: public

text: Staph pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staph pseudintermedius

text: CP146084/04 pred-1

text: SC

text: public

text: Staph pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staph pseudintermedius

text: CP147691 White-1

text: SC

text: public

text: Staph pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staph pseudintermedius

text: CP147901 Pred-1

text: SC

text: public

text: Staph pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staphylococcus aureus

text: CP147901 Occ-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus warneri

text: SC

text: public

text: Staphylococcus warneri

text: Staphylococcus warneri

text: Staphylococcus warneri


text: Staphylococcus aureus

text: CP14922401 MSA-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus schleiferi

text: SC

text: public

text: Staphylococcus schleiferi

text: Staphylococcus schleiferi

text: Staphylococcus schleiferi


text: Staphylococcus warneri

text: CP149526 Occ-1

text: SC

text: public

text: Staphylococcus warneri

text: Staphylococcus warneri

text: Staphylococcus warneri


text: Staphylococcus aureus

text: CP15015403 Pred-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus epidermidis

text: CP149567 grey-1

text: SC

text: public

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis


text: Staphylococcus aureus

text: CP15013102 Lac-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15013101 Lac-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15013103 Lac-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15015403 Occ-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150327 01 MSA-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150327 01 Cled-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150327 02 MSA-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150327 02 Cled-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150328 01 Cled-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150328 01 MSA-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150328 02 Bright-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150328 02 Pale-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP150328 03-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus warneri

text: CP151222 White-1

text: SC

text: public

text: Staphylococcus warneri

text: Staphylococcus warneri

text: Staphylococcus warneri


text: Staphylococcus epidermidis

text: SC

text: public

text: Staphylococcus epidermidis

text: Staphylococcus epidermidis

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text: Staphylococcus pseudintermedius

text: SC

text: public

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius

text: Staphylococcus pseudintermedius


text: Staphylococcus aureus

text: CP15160901 pred-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15160902 pred-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15160902 hame-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15176701 pred-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15176701 Occ-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15176702 pred-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus aureus

text: CP15176803 Lg-1

text: SC

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: Staphylococcus sciuri

text: 3F h1 32

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


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text: 9N h1 30

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

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text: SC

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text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 16B h1 22

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 17N h1 19

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 20F h1 39

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: Staphylococcus sciuri

text: 18B h1 33

text: SC

text: public

text: Staphylococcus sciuri

text: Mammaliicoccus sciuri

text: Mammaliicoccus sciuri


text: public

tag: Namespace:Department of Surgery; Wellcome - MRC Cambridge Stem Cell Institute

text: ArrayExpress

characteristic: broker name

text: hepatocyte-like cell

text: human

text: Protocols: hiPSCs were differentiated into hepatocyte-like cells in 2D for 23 days. Cells were dissociated in clumps and embedded in the 3D collagen system for 7 days. Cells were treated with BSA (Control), oleic acid (OA) or palmitic acid (PA) for 24h or up to 1 week. RNA was collected at these time points. Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) following manufacturer's instructions. The libraries for RNA-sequencing were generated by the Wellcome Sanger Institute High-Throughput RNA Sequencing Team using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.

characteristic: description

text: wild-type (corrected in vitro)

characteristic: genotype

text: 3D culture

characteristic: growth condition

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

characteristic: progenitor cell type

characteristic: replicate

text: Homo sapiens

unit: week

characteristic: time


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Surgery; Wellcome - MRC Cambridge Stem Cell Institute

text: public

text: ArrayExpress

text: hepatocyte-like cell

text: human

text: wild-type (corrected in vitro)

text: 3D culture

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: hepatocyte-like cell

text: human

text: wild-type (corrected in vitro)

text: 3D culture

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

unit: hour


text: Streptococcus agalactiae

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Streptococcus agalactiae

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: public

tag: Namespace:Department of Biosciences and Nutrition, Karolinska Institutet

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

characteristic: common name

text: Protocols: Doxycycline-pulsed and control DUX4-TetOn hESCs were collected for scRNA-seq after 6 h, 12 h, 24 h, and 48 h incubation times after a 15 min doxycycline or without doxycycline treatment. The cells were washed once with PBS and incubated with TrypLE Express (Thermo Fisher Scientific) for 4 min. TrypLE was diluted with Essential 8 medium and the cell suspensions were filtered through 40 µm Cell Strainers. Cell suspensions were centrifuged at 400 rcf for 8 min. Cell pellets were resuspended each in 100 µl of Dead Cell Removal Kit microbeads (Miltenyi Biotec) and incubated at room temperature for 15 min. After incubation, each cell-microbead suspension was gently resuspended to 800 µl of freshly prepared 1× Binding buffer. Cell suspensions were pipetted to magnetic MS columns (Miltenyi Biotec) 500 µl at a time and let flow through. The columns were washed three times with 1× Binding buffer. The cell suspensions were centrifuged at 400 rcf for 5 min, and the pellets were resuspended each in 400 µl of 10x Genomics sample buffer. The cells were counted and the volumes were adjusted to approximately 1,200 cells/µl of suspension. RNA extraction was performed per in chip inside 10x chromium controller. Libraries were prepared using Chromium Next GEM Single Cell 3’ Gene Expression version 3.1 chemistry.

characteristic: description

text: embryo stage

characteristic: developmental stage

text: TetOn DUX4-full-length

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex

text: 15 min doxycycline-induction

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Biosciences and Nutrition, Karolinska Institutet

text: public

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo stage

text: TetOn DUX4-full-length

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: female

text: 15 min doxycycline-induction

characteristic: title


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: embryonic stem cell

text: human

text: embryo stage

text: TetOn DUX4-full-length

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: Homo sapiens

text: female


text: KLE TEch

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:KLE TEch

characteristic: Submitter Id

text: rice

text: VM3 DESasaasa

text: Oryza sativa

text: Oryza sativa

text: AMMMSMSMsaasasas


text: not applicable

characteristic: ArrayExpress-Sex

text: metagenome

characteristic: ArrayExpress-Species

text: SC

text: public

text: metagenome

characteristic: common name

text: metagenome

text: Metagenomic bacteriophage DNA with human carrier DNA

text: metagenome

characteristic: strain


text: not applicable

characteristic: ArrayExpress-SEX

text: metagenome

characteristic: ArrayExpress-SPECIES

text: Not Applicable

characteristic: GENDER

text: SC

text: public

text: metagenome

text: metagenome

text: metagenome


text: not applicable

text: metagenome

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: metagenome

text: metagenome

text: Human carrier DNA

text: metagenome


text: not applicable

text: metagenome

characteristic: External Id

text: SC

text: public

text: metagenome

text: metagenome

characteristic: organism

text: Metagenomic viral DNA with human carrier DNA

text: metagenome

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Wellcome - MRC Cambridge Stem Cell Institute and Department of Surgery, University of Cambridge

text: ArrayExpress

characteristic: broker name

text: primary human hepatocytes

text: fresh hepatocytes

text: human

text: Protocols: Primary human hepatocytes were sourced from Biopredic International as monolayer plated cultures (pPHHs), or freshly isolated (fPHHs) from tissue donors as previously reported (Segeritz et al, 2018). RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Wellcome Trust Sanger Institute DNA Sequencing Facility (Hinxton, UK).

characteristic: description

text: adult tissue

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: liver

characteristic: organism part

characteristic: replicate

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: Wellcome - MRC Cambridge Stem Cell Institute and Department of Surgery, University of Cambridge

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary human hepatocytes

text: fresh hepatocytes

text: human

characteristic: common name

text: adult tissue

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: hepatocyte-like cells

text: human

text: Protocols: Hepatocyte-like cells (HLCs) were differentiated from hESCs or hIPSCs as previously reported (Hannah et al, 2013; Segeritz et al, 2018). Undifferentiated hIPSCs were maintained in Essential 8 medium. RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Cambridge Genomic Services (Cambridge, UK).

text: Differentiated in vitro from embryonic stem cells

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: cell line

text: Homo sapiens

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: induced pluripotent stem cells

text: human

text: Protocols: Hepatocyte-like cells (HLCs) were differentiated from hESCs or hIPSCs as previously reported (Hannah et al, 2013; Segeritz et al, 2018). Undifferentiated hIPSCs were maintained in Essential 8 medium. RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Wellcome Trust Sanger Institute DNA Sequencing Facility (Hinxton, UK).

characteristic: description

text: induced pluripotent stem cells

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: cell line

text: Homo sapiens


text: public

text: ArrayExpress

text: hepatocyte-like cells

text: human

text: Differentiated in vitro from induced pluripotent stem cells

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype (corrected in vitro)

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: cell line

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: primary human hepatocytes

text: cultured hepatocytes

text: human

text: Protocols: Primary human hepatocytes were sourced from Biopredic International as monolayer plated cultures (pPHHs), or freshly isolated (fPHHs) from tissue donors as previously reported (Segeritz et al, 2018). RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Cambridge Genomic Services (Cambridge, UK).

text: adult tissue

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: 1 (technical replicate 1)

characteristic: replicate

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: primary human hepatocytes

text: cultured hepatocytes

text: human

characteristic: common name

text: adult tissue

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: 1 (technical replicate 2)

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: primary human hepatocytes

text: cultured hepatocytes

text: human

text: adult tissue

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: liver

text: 2 (technical replicate 1)

text: Homo sapiens

characteristic: title


text: public

text: ArrayExpress

text: primary human hepatocytes

text: cultured hepatocytes

text: human

characteristic: description

text: adult tissue

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: liver

text: 2 (technical replicate 2)

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Hepatocyte-like cells

text: human

text: Protocols: Chromatin was crosslinked with 1% formaldehyde for 10 minutes at RT and quenched with 0.125M glycine. Cells and nuclei were lysed and chromatin was sonicated to fragment DNA to about 200-500 bp on a Bioruptor Pico sonication device (Diagenode). Sonicated chromatin was pre-cleared with same-host IgG and protein G Dynabeads. For immunoprecipitation, 100µg of chromatin was incubated with 2µg of each antibody: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam, ab8895), H3K27me3 (Active Motif, 39155) and H3K4me3 (Merk, 05-745R) overnight at 4C and complexes captured with 30µl of protein G Dynabeads. Complexes were washed, RNAse and Proteinase K treated. DNA was purified by phenol-chloroform extraction. A sonicated chromatin sample (1%) was also collected as input for normalisation. One input (input 1) was collected for H3K27ac+H3K4me1 immunoprecipitation and a second input (input 2) for H3K27me3+H3K4me3. For hIPSC and hESC derived hepatocyte-like cells (HLCs), 2 independent differentiation experiments were used. Cells were differentiated into HLCs for 30 days has previously reported (Hannan et al, 2013) with minor modifications. Following 3 days of endoderm differentiation, anterior foregut specification was achieved with RPMI-B27 differentiation media supplemented with 50ng/ml Activin A (R&D) for 5 days. Cells at the foregut stage were further differentiated into hepatocytes with Hepatozyme complete medium: HepatoZYME-SFM (Thermo Fisher) supplemented with 2mM L-glutamine (Thermo Fisher), 1% penicillin-streptomycin (Thermo Fisher), 2% non-essential aminoacids (Thermo Fisher), 2% chemically defined lipids (Thermo Fisher), 14μg/ml of insulin (Roche), 30μg/ml of transferrin (Roche), 50 ng/ml hepatocyte growth factor (R&D), and 20 ng/ml oncostatin M (R&D), for up to day 30 of differentiation. Adult primary human hepatocytes (PHHs) were purchased from Biopredic International as a monolayer culture. One biological replicate was used and immunoprecipitated as 2 separate technical replicates. Immunoprecipitated DNA was purified by phenol-chloroform extraction and precipitated with GlycoBlue (Thermo Fisher), sodium acetate (Thermo Fisher) and ethanol (Sigma-Aldrich). Library preparation was performed by the Wellcome Trust Sanger Institute DNA Sequencing Facility (Hinxton, UK). ChIP-sequencing libraries were generated using 10ng of DNA and 8 PCR cycles for all samples.

text: differentiated in vitro from embryonic stem cells

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: cell line

characteristic: organism part

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: Hepatocyte-like cells

text: human

characteristic: common name

text: differentiated in vitro from induced pluripotent stem cells

text: normal

text: wild type genotype (corrected in vitro)

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: cell line

characteristic: replicate

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: primary human hepatocytes

text: Primary hepatocytes

text: human

text: adult tissue

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: liver

text: Homo sapiens


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: strain

characteristic: title


text: Plasmodium falciparum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: UC Irvine

text: public

characteristic: SRA accession

tag: Namespace:UC Irvine

characteristic: Submitter Id

text: ArrayExpress

text: postman butterfly

text: Protocols: Tissues were dissected from butterfly heads. Nuclei were extracted using a cell lysis buffer and by manual grounding. We did a transposition reaction with Tn5 Transposase followed by a cleanup and PCR reaction.

characteristic: description

text: adult

characteristic: developmental stage

text: not applicable

characteristic: isolate

text: Heliconius melpomene

text: Heliconius melpomene

text: brain

characteristic: organism part

text: Heliconius melpomene

text: female

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UC Irvine

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: postman butterfly

characteristic: common name

text: adult

text: not applicable

text: Heliconius melpomene

text: Heliconius melpomene

text: brain

text: Heliconius melpomene

text: male


text: UC Irvine

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: postman butterfly

text: adult

text: not applicable

text: Heliconius melpomene

characteristic: organism

text: Heliconius melpomene

text: eye

text: Heliconius melpomene

text: female

characteristic: title


characteristic: ENA-CHECKLIST

text: UC Irvine

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: postman butterfly

text: adult

text: not applicable

text: Heliconius melpomene

text: Heliconius melpomene

text: eye

text: Heliconius melpomene

text: male


text: Mus musculus

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus


text: public

tag: Namespace:Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit

text: 147 to 167

unit: day

characteristic: age

text: ArrayExpress

text: intact

characteristic: clinical information

text: pig

text: Protocols: Tissue samples were dissected from the animals following an anatomical reference guide. The samples were immediately flash frozen in liquid nitrogen and kept at -80 °C until DNA extraction. All animal care and sample collection procedures were in compliance with the German Animal Protection Law and approved by the FBN animal care committee. The r nomenclature in the files stands for run not read. Files are single end technical replicates. Genomic DNA was extracted from samples using a DNeasy kit (Qiagen) according to the manufacturer’s recommendation including proteinase K digestion and RNase A treatment to obtain high purity DNA. 2 µg of DNA was spiked-in with 1% DNA control of unmethylated cl857 Sam7 Lambda DNA (Promega) and digested at 37 °C for 7 hours with 100U MspI (New England Biolabs) and then at 65 °C overnight with 50U TaqαI (New England Biolabs). The DNA fragments were end-repaired, A-tailed and ligated to TruSeq DNA adapters using a TruSeq Nano DNA library prep kit (Illumina) according to the manufacturer’s protocols. Size-selection of the adapter-ligated DNA fragments were achieved by PAGE to recover DNA inserts around 40-240bp. The adapter-ligated DNA libraries was subjected to a bisulfite conversion using EpiTect Bisulfite kit (Qiagen) according to the manufacturer’s protocols. Library amplification was performed using a PfuTurbo Cx Hotstart DNA Polymerase kit (Stratagene). The quality of libraries was assessed on 2100 Bioanalyzer using a high-sensitive DNA chip (Agilent Technologies).

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: amygdala

characteristic: organism part

text: Sus scrofa

text: female

text: German Landrace

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 147 to 167

text: ArrayExpress

text: castrated

text: pig

characteristic: common name

text: adult

text: wild type genotype

text: Sus scrofa

text: Sus scrofa

text: amygdala

text: Sus scrofa

text: male

characteristic: sex

text: German Landrace


characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 147 to 167

text: ArrayExpress

characteristic: broker name

text: intact

text: pig

text: adult

text: wild type genotype

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: amygdala

text: Sus scrofa

text: female

text: German Landrace

characteristic: title


text: public

characteristic: Submitter Id

text: 147 to 167

characteristic: age

text: ArrayExpress

text: intact

characteristic: clinical information

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: hippocampus

text: Sus scrofa

text: female

text: German Landrace


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: 147 to 167

text: ArrayExpress

text: castrated

text: pig

text: adult

text: wild type genotype

text: Sus scrofa

text: Sus scrofa

text: hippocampus

characteristic: organism part

text: Sus scrofa

text: male

text: German Landrace

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 147 to 167

text: ArrayExpress

text: intact

text: pig

characteristic: common name

text: adult

text: wild type genotype

text: Sus scrofa

text: Sus scrofa

text: hypothalamus

text: Sus scrofa

text: female

characteristic: sex

text: German Landrace


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 147 to 167

characteristic: age

text: ArrayExpress

characteristic: broker name

text: castrated

text: pig

text: adult

text: wild type genotype

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: hypothalamus

text: Sus scrofa

text: male

text: German Landrace

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: 147 to 167

text: ArrayExpress

text: intact

characteristic: clinical information

text: pig

characteristic: description

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Sus scrofa

text: Sus scrofa

text: adrenal gland

text: Sus scrofa

text: female

text: German Landrace


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 147 to 167

text: ArrayExpress

text: castrated

text: pig

text: adult

text: wild type genotype

text: Sus scrofa

text: Sus scrofa

text: adrenal gland

characteristic: organism part

text: Sus scrofa

text: male

text: German Landrace

characteristic: strain


E-MTAB-10693:Sample 1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 1

tag: Namespace:University of Kent School of Biosciences

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Whole day 1 adult C. elegans collected from nematode growth medium plates with 1 of 3 bacterial lawn conditions: E. coli OP50 (control), experimental microbiome, or OP50 supplemented with cell-free supernatant from experimental microbiome RNA extraction with Trizol and BCP TruSeq mRNA stranded library preparation method using standard TruSeq adapters

text: adult

text: E. coli OP50

characteristic: diet

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 1


E-MTAB-10693:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Kent School of Biosciences

text: public

text: E-MTAB-10693:Sample 2

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 2

characteristic: title


E-MTAB-10693:Sample 3

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 3

text: ArrayExpress

text: adult

text: E. coli OP50

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 3


E-MTAB-10693:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 5


E-MTAB-10693:Sample 4

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 4

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 4

characteristic: title


E-MTAB-10693:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 6

text: ArrayExpress

text: adult

text: E. coli OP50

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: cell free medium from experimental microbiome

characteristic: strain

text: Sample 6


E-MTAB-10693:Sample 7

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 7

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 7


E-MTAB-10693:Sample 8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 8

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 8

characteristic: title


E-MTAB-10693:Sample 9

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 9

text: ArrayExpress

text: adult

text: E. coli OP50

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 9


E-MTAB-10693:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 10


E-MTAB-10693:Sample 11

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 11

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: experimental microbiome

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 11

characteristic: title


E-MTAB-10693:Sample 12

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 12

text: ArrayExpress

text: adult

text: experimental microbiome

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 12


E-MTAB-10693:Sample 13

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 13

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: experimental microbiome

characteristic: diet

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 13


E-MTAB-10693:Sample 14

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 14

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: experimental microbiome

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 14

characteristic: title


E-MTAB-10693:Sample 15

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 15

text: ArrayExpress

text: adult

text: experimental microbiome

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: amyloid beta 1-42 expression

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 15


E-MTAB-10693:Sample 16

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 16

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

text: wild type genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: wild type

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 16


E-MTAB-10693:Sample 17

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 17

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 17

characteristic: title


E-MTAB-10693:Sample 18

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 18

text: ArrayExpress

text: adult

text: E. coli OP50

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 18


E-MTAB-10693:Sample 19

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

text: wild type genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: wild type

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 19


E-MTAB-10693:Sample 20

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 20

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 20

characteristic: title


E-MTAB-10693:Sample 21

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 21

text: ArrayExpress

text: adult

text: E. coli OP50

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 21


E-MTAB-10693:Sample 22

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 22

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

text: wild type genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: wild type

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 22


E-MTAB-10693:Sample 23

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 23

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: E. coli OP50

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 23

characteristic: title


E-MTAB-10693:Sample 24

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 24

text: ArrayExpress

text: adult

text: E. coli OP50

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 24


E-MTAB-10693:Sample 25

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 25

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: E. coli OP50

characteristic: diet

text: wild type genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: wild type

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 25


E-MTAB-10693:Sample 26

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 26

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: experimental microbiome

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 26

characteristic: title


E-MTAB-10693:Sample 27

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 27

text: ArrayExpress

text: adult

text: experimental microbiome

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 27


E-MTAB-10693:Sample 28

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10693:Sample 28

characteristic: Submitter Id

text: ArrayExpress

text: adult

text: experimental microbiome

characteristic: diet

text: wild type genotype

characteristic: individual

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: wild type

characteristic: phenotype

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

characteristic: stimulus

text: Sample 28


E-MTAB-10693:Sample 29

characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

text: E-MTAB-10693:Sample 29

characteristic: age

text: ArrayExpress

characteristic: broker name

characteristic: description

text: adult

characteristic: developmental stage

text: experimental microbiome

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

text: Sample 29

characteristic: title


E-MTAB-10693:Sample 30

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10693:Sample 30

text: ArrayExpress

text: adult

text: experimental microbiome

text: wild type genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: wild type

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Sample 30


E-MTAB-10705:Sample 1

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 1

tag: Namespace:UNIVERSITY OF BERGEN

characteristic: Submitter Id

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: common name

text: Protocols: Retinas were isolated from E18.5, C57BL6 mouse embryos and digested with 0.05% trypsin (Gibco) for 15 minutes at 37 C. After 1 day of culture, cells were starved for glucose in Neobasal medium supplemented with B27 supplement without insulin, 0.06 g/L glutamine, 1% penicillin-streptomycin (Gibco) and 1 mM HEPES for 6 h. The cells were harvested for RNA isolation after additional 6 days of culturing. Normal cultured cells were used as control. RNA extracts were isolated by using TruSeq Stranded Total RNA with RiboZero. Reverse transcription was done by using RevertAid first strand cDNA synthesis kit (ThermoFisher)

text: wild type genotype

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

characteristic: sex

text: Sample 1


E-MTAB-10705:Sample 10

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 10

text: ArrayExpress

characteristic: broker name

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: description

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: glucose starvation

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 10

characteristic: title


E-MTAB-10705:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BERGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10705:Sample 11

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

text: wild type genotype

text: glucose starvation

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 11


E-MTAB-10705:Sample 12

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF BERGEN

text: public

characteristic: SRA accession

text: E-MTAB-10705:Sample 12

characteristic: Submitter Id

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

text: wild type genotype

text: glucose starvation

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

characteristic: organism part

text: Mus musculus

text: male

characteristic: strain

text: Sample 12


E-MTAB-10705:Sample 2

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 2

text: ArrayExpress

characteristic: broker name

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: common name

characteristic: description

text: wild type genotype

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

characteristic: sex

text: Sample 2


E-MTAB-10705:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 3

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 3

characteristic: title


E-MTAB-10705:Sample 4

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF BERGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10705:Sample 4

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 4


E-MTAB-10705:Sample 5

text: UNIVERSITY OF BERGEN

text: public

characteristic: SRA accession

text: E-MTAB-10705:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: common name

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

characteristic: organism part

text: Mus musculus

text: female

characteristic: strain

text: Sample 5


E-MTAB-10705:Sample 6

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 6

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: description

text: wild type genotype

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

characteristic: sex

text: Sample 6


E-MTAB-10705:Sample 7

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 7

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: glucose starvation

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 7

characteristic: title


E-MTAB-10705:Sample 8

text: UNIVERSITY OF BERGEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10705:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: cultured retinal cells

text: retinal cell

text: house mouse

text: wild type genotype

text: glucose starvation

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

text: Mus musculus

text: male

text: Sample 8


E-MTAB-10705:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF BERGEN

text: public

text: E-MTAB-10705:Sample 9

text: ArrayExpress

characteristic: broker name

text: cultured retinal cells

text: retinal cell

text: house mouse

characteristic: common name

text: wild type genotype

text: glucose starvation

text: not applicable

text: Mus musculus

text: Mus musculus

text: retina

characteristic: organism part

text: Mus musculus

text: female

characteristic: strain

text: Sample 9


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Univ Montpellier INRAE

text: public

tag: Namespace:Univ Montpellier INRAE

text: ArrayExpress

text: fall armyworm

text: Protocols: Sf9 cells are grown in F75 plates. They were seeded with 3E5 cells/mL/well. When confluence is reached, the medium was removed from the plates and cells were rinsed with cold 1X PBS. Cells were then scraped from the plate in 1mL 1X cold PBS. Cells were centrifuged for 2 minutes at 1500 rpm at 4°C. The supernatant was removed and the pellet resuspended in 300µl of TE buffer supplemented with Complete Mini Protease inhibitor cocktail. Cells were lyzed by sonication with a Diagenode Bioruptor (3 cycles, 30\" ON; 30\" OFF). Sf9 cells from ATCC are cultured at 28°C according to manufacturer recommandations. \"The base medium for this cell line is Grace's Insect Medium Supplemented (GIBCO/Invitrogen Cat. No. 11605-094 or equivalent). To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum (Sigma cat# F4135) to a final concentration of 10%.\" RNA was extracted with the RNEasy kit from Qiagen and treated with turboDNAse (Ambion). Total RNA was used for RNA-seq libraries. Library preparation and RNA sequencing were conducted by MGX GenomiX (IGF, Montpellier, France). Libraries were prepared with the TruSeq Stranded mRNA Sample preparation kit (Illumina). In brief, after a purification step with oligo(dT) magnetic beads, polyadenylated RNAs were chemically fragmented. A first cDNA strand was synthesized with random primers and SuperScript IV Reverse Transcriptase (Life Technologies) and the second strand was then synthesized. After the addition of single adenine nucleotides, indexed adapters were ligated to the cDNA ends. Adapters-ligated cDNAs were then amplified by PCR and libraries were validated on Fragment Analyzer with a Standard Sensitivity NGS kit (Advanced Analytical Technologies, Inc) and quantified by qPCR with a Light Cycler 480 thermal cycler (Roche Molecular diagnostics).

characteristic: description

text: adult

text: not applicable

characteristic: isolate

text: Spodoptera frugiperda

text: Spodoptera frugiperda

text: insect ovary

text: Spodoptera frugiperda

text: female

characteristic: sex

text: corn


text: Univ Montpellier INRAE

characteristic: INSDC center name

text: public

text: ArrayExpress

text: epithelial cell

text: fall armyworm

text: Protocols: Sf9 cells are grown in F75 plates. They were seeded with 3E5 cells/mL/well. When confluence is reached, the medium was removed from the plates and cells were rinsed with cold 1X PBS. Cells were then scraped from the plate in 1mL 1X cold PBS. Cells were centrifuged for 2 minutes at 1500 rpm at 4°C. The supernatant was removed and the pellet resuspended in 300µl of TE buffer supplemented with Complete Mini Protease inhibitor cocktail. Cells were lyzed by sonication with a Diagenode Bioruptor (3 cycles, 30\" ON; 30\" OFF). Biological samples are being crushed in a douncer, in presence of 1% formaldehyde and incubated for 15 minutes to allow protein-DNA crosslinking. Crosslinking was quenched by the addition of 225mM glycin. Chromatin was then fragmented using a Bioruptor sonicator (Diagenode). An aliquot of sonicated chromatin at this stage is used as the Input sample. For the immunoprecipitation, sonicated chromatin is incubated for 4 h with the primary antibody at 4°C, then with Protein-A sepharose beads (CL4B) for again 4 h. Beads were then centrifuged and washed. Chromatin was eluted from the beads with a 50mM Tris-HCl buffer with 1%SDS and 10mM EDTA. To reverse the cross-linking, this immuno-precipitate was incubated overnight at 65°C and then 2-3h at 50°C with proteinase K. DNA was purified from this precipitate with 500µL of phenol-chloroform and 55µL of 4M LiCl. The DNA from the aqueous phase is precipitated with 100% ethanol and the pellet dried and resuspended in water. Chromatin was prepared from 50 L4 larvae pool and 50 mL of confluent cells per IP condition. Two biological replicates for input and ChIP conditions have been systematically produced except for larvae (1 replicate only). Library preparation were conducted by MGX GenomiX (IGF, Montpellier, France). Libraries were prepared with the TruSeq ChIP Sample preparation kit (Illumina). It contains 5 steps. 1-end-repair of DNA fragments. 2-Adenine addition at DNA ends. 3-Adapters ligation. 4-Adapters-ligated DNAs were then amplified by PCR. Only fragments containing two types of adapters are selected. 5-Purification on agarose gel 2%. Libraries were validated on Fragment Analyzer with a High Sensitivity NGS kit (Advanced Analytical Technologies, Inc) and quantified by qPCR with a Light Cycler 480 thermal cycler (Roche Molecular diagnostics).

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

text: Spodoptera frugiperda

characteristic: organism

text: Spodoptera frugiperda

text: insect ovary

text: Spodoptera frugiperda

text: female

text: corn strain

characteristic: title


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Novo Nordisk Foundation Center for Stem Cell Biology, University of Copenhagen

characteristic: Submitter Id

text: ArrayExpress

text: foregut progenitor cell

text: human

text: Protocols: Cells were washed once in PBS, and RNA was harvested in RLT plus. Each cell line was seeded at 150.000cells/cm2 in 12-well plates. Differentiation (Rezania et al., 2014) was performed thrice for HUES4 and twice for H1 hESCs. IWP-L6 (50 nM) and/or TGF-b1 (10 ng/ml) was added from day 3 to day 7 and cells were harvested for analysis at day 5 and day 7. RNA was extracted using RNeasy Mini plus kit on a Qiacube (Qiagen). NEBNext Ultra II RNA Library Prep Kit - Module #E7770S NEBNext PolyA mRNA Magnetic Isolation Module #E7490 NEBNext multiplex Oligos for Illumina. RNA-seq libraries were built from 1µg of RNA using the NEB NEXT Ultra II with mRNA magnetic isolation module (NEB #E7775 and #E7490) with 5 cycles of amplification. Quality of the RNA and subsequently of the libraries were measured on a Fragment Analyser.

text: embryo

text: normal

characteristic: disease

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

characteristic: progenitor cell type

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Novo Nordisk Foundation Center for Stem Cell Biology, University of Copenhagen

text: public

text: ArrayExpress

characteristic: broker name

text: foregut progenitor cell

text: human

characteristic: common name

text: embryo

text: normal

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

characteristic: organism part

text: embryonic stem cell

text: Homo sapiens


characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: pancreatic progenitor cell

text: human

characteristic: description

text: embryo

characteristic: developmental stage

text: normal

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: inner cell mass

text: embryonic stem cell

text: Homo sapiens


characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:West of Scotland Specialist Virology Centre, NHSGGC / MRC-University of Glasgow Centre for Virus Research

text: COVID-19 Genomics UK Consortium

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

characteristic: title

text: not provided

characteristic: virus identifier


E-MTAB-10738:MCF7 ERaLBDoe+Fulv #1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10738:MCF7 ERaLBDoe+Fulv #1

tag: Namespace:Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: common name

text: Protocols: Cultured breast cancer cells were harvested and cell pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation. Library preparation was performed by the sequencing contractor Genewiz (NJ, USA).

characteristic: description

text: breast cancer

characteristic: disease

text: ERaLBD overexpression

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: MCF7 ERaLBDoe+Fulv #1


E-MTAB-10738:MDA-MB-231 NC #1

characteristic: ENA-CHECKLIST

text: Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

text: public

text: E-MTAB-10738:MDA-MB-231 NC #1

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-231 NC #1

characteristic: title


E-MTAB-10738:MCF7 ERaLBDoe+Fulv #2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-10738:MCF7 ERaLBDoe+Fulv #2

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: ERaLBD overexpression

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MCF7 ERaLBDoe+Fulv #2


E-MTAB-10738:MCF7 FulvRes+Fulv #1

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-10738:MCF7 FulvRes+Fulv #1

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: Fulvestrant resistant

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MCF7 FulvRes+Fulv #1


E-MTAB-10738:MCF7 FulvRes+Fulv #2

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10738:MCF7 FulvRes+Fulv #2

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: Fulvestrant resistant

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MCF7 FulvRes+Fulv #2


E-MTAB-10738:MCF7 NC+Fulv #1

text: public

text: E-MTAB-10738:MCF7 NC+Fulv #1

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MCF7 NC+Fulv #1


E-MTAB-10738:MDA-MB-453 ERaLBDkd #1

text: public

text: E-MTAB-10738:MDA-MB-453 ERaLBDkd #1

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

characteristic: disease

text: ERaLBD knockdown

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-453 ERaLBDkd #1


E-MTAB-10738:MCF7 NC+Fulv #2

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10738:MCF7 NC+Fulv #2

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: MCF7 NC+Fulv #2


E-MTAB-10738:MDA-MB-231 ERaLBDkd #1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10738:MDA-MB-231 ERaLBDkd #1

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: ERaLBD knockdown

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-231 ERaLBDkd #1

characteristic: title


E-MTAB-10738:MDA-MB-453 ERaLBDkd #2

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10738:MDA-MB-453 ERaLBDkd #2

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: ERaLBD knockdown

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-453 ERaLBDkd #2


E-MTAB-10738:MDA-MB-231 ERaLBDkd #2

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10738:MDA-MB-231 ERaLBDkd #2

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: ERaLBD knockdown

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-231 ERaLBDkd #2


E-MTAB-10738:MDA-MB-453 NC #1

text: public

text: E-MTAB-10738:MDA-MB-453 NC #1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: common name

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-453 NC #1


E-MTAB-10738:MDA-MB-231 NC #2

text: public

text: E-MTAB-10738:MDA-MB-231 NC #2

text: ArrayExpress

text: epithelial cell

text: human

characteristic: description

text: breast cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-231 NC #2


E-MTAB-10738:MDA-MB-453 NC #2

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10738:MDA-MB-453 NC #2

text: ArrayExpress

text: epithelial cell

text: human

text: breast cancer

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: MDA-MB-453 NC #2


E-MTAB-10744:Control rep1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10744:Control rep1

tag: Namespace:Department for Gynecology and Obstetrics.

text: ArrayExpress

text: epithelial cell

text: human

text: Protocols: HCC1806 cells were treated with siRNA against ROBO3s in 6-well plates for 72 hours in biological triplicates. Total RNA was extracted from HCC1806 cells with Qiazol (Qiagen) following the manufacturer's recommendations. mRNA sequencing libraries were generated with the Illumina Trueseq v2 Kit according to the manufacturer’s instructions. The size of the generated libraries was estimated on High Sensitivity DNA chip (Agilent) using a Bioanalyser 2100 (Agilent).

text: adult

characteristic: developmental stage

text: squamous cell breast carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Control rep1


E-MTAB-10744:siROBO3 rep3

tag: attribute

characteristic: External Id

text: Department for Gynecology and Obstetrics.

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10744:siROBO3 rep3

text: ArrayExpress

text: epithelial cell

text: human

text: adult

text: squamous cell breast carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: siROBO3 rep3

characteristic: title


E-MTAB-10744:siROBO3 rep1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10744:siROBO3 rep1

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: adult

text: squamous cell breast carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: siROBO3 rep1


E-MTAB-10744:siROBO3 rep2

text: public

text: E-MTAB-10744:siROBO3 rep2

text: ArrayExpress

characteristic: broker name

text: epithelial cell

text: human

characteristic: common name

characteristic: description

text: adult

text: squamous cell breast carcinoma

characteristic: disease

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: siROBO3 rep2


E-MTAB-10744:Control rep3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10744:Control rep3

text: ArrayExpress

text: epithelial cell

text: human

text: adult

text: squamous cell breast carcinoma

text: wild type genotype

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

text: Control rep3


E-MTAB-10744:Control rep2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10744:Control rep2

text: ArrayExpress

text: epithelial cell

text: human

text: adult

characteristic: developmental stage

text: squamous cell breast carcinoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens

text: Control rep2


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

text: Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay.

text: adult

text: adenocarcinoma

text: in vitro

characteristic: growth condition

text: ER negative

characteristic: immunophenotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: adult

text: metastatic carcinoma

text: in vitro

text: ER negative

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: human

characteristic: description

text: adult

text: breast cancer

characteristic: disease

text: patient derived xenograft

text: ER negative

text: not applicable

characteristic: isolate

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: human

text: adult

characteristic: developmental stage

text: adenocarcinoma

text: in vitro

text: ER positive

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

text: adult

text: breast cancer

text: patient derived xenograft

characteristic: growth condition

text: ER positive

characteristic: immunophenotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: adult

text: breast ductal adenocarcinoma

text: in vitro

text: ER positive

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


E-MTAB-10750:clone #6

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-10750:clone #6

tag: Namespace:Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the Czech Academy of Sciences

text: ArrayExpress

text: U-2 OS

text: osteosarcoma cell

text: human

characteristic: common name

text: Protocols: Cells were harvested from respective cell cultures. Human fibroblasts were cultured in Minimum Essential Medium Eagle (MEM; Gibco) supplemented with 15 % fetal bovine serum (FBS; Gibco), 2 mM L-glutamine (Gibco), and the antibiotics penicillin (100 units/ml) and streptomycin (100 μg/ml) (Pen/Strep; Gibco) at 37°C. Human wild type osteosarcoma cells (U2OS) were cultured in Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM-high glucose; Sigma) supplemented with 10 % FBS and the antibiotics Pen/Strep at 37°C. BRAT1 KO gene edited U2OS cell lines were prepared using Cas9 and guide sequences designed in CRISPR direct (https://crispr.dbcls.jp). Total RNA was isolated from 1×106 cells by miRNeasy Micro Kit (Qiagen) with an additional DNase I treatment, according to the manufacturer’s instructions. Sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian user manual (Takara Bio USA, Inc).

characteristic: description

text: adult

text: osteosarcoma

characteristic: disease

characteristic: familial relationship

text: CRISPR/Cas9 mediated BRAT1 knockout

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: bone

text: Homo sapiens

text: female

characteristic: sex

text: clone #6


E-MTAB-10750:Patient 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the Czech Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10750:Patient 2

text: ArrayExpress

text: B lymphoblast

text: B lymphoblast

text: human

text: adult

characteristic: developmental stage

text: cerebellar ataxia

text: proband

text: BRAT1 V62E/V62E

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: peripheral blood

characteristic: organism part

text: Homo sapiens

text: male

text: Patient 2


E-MTAB-10750:WT #1

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10750:WT #1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: U-2 OS

text: osteosarcoma cell

text: human

text: adult

text: osteosarcoma

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: bone

text: Homo sapiens

text: female

text: WT #1

characteristic: title


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: B lymphoblast

text: B lymphoblast

text: human

characteristic: common name

characteristic: description

text: adult

text: normal

characteristic: disease

text: mother

characteristic: familial relationship

text: BRAT1 V62E/+

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: female

text: Mother


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: B lymphoblast

text: B lymphoblast

text: human

text: adult

text: normal

text: father

text: BRAT1 V62E/+

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

characteristic: sex

text: Father


E-MTAB-10750:WT #2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10750:WT #2

text: ArrayExpress

text: U-2 OS

text: osteosarcoma cell

text: human

text: adult

characteristic: developmental stage

text: osteosarcoma

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone

characteristic: organism part

text: Homo sapiens

text: female

text: WT #2


E-MTAB-10750:Patient 1

characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

text: E-MTAB-10750:Patient 1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: B lymphoblast

text: B lymphoblast

text: human

characteristic: common name

text: adult

text: cerebellar ataxia

text: proband

text: BRAT1 V62E/V62E

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: peripheral blood

text: Homo sapiens

text: male

text: Patient 1

characteristic: title


E-MTAB-10750:clone #8

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-10750:clone #8

text: ArrayExpress

text: U-2 OS

text: osteosarcoma cell

text: human

characteristic: description

text: adult

text: osteosarcoma

characteristic: disease

characteristic: familial relationship

text: CRISPR/Cas9 mediated BRAT1 knockout

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: bone

text: Homo sapiens

text: female

text: clone #8