characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Verrucomicrobia bacterium

characteristic: organism

text: Metagenomic Analysis of Colonic Microbiome of Landrace Finishing Pigs with High and Low Feed Efficiency

text: uncultured Verrucomicrobia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976185_bin.8_metaWRAP_v1.3_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5976185 of study SRP116179.

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

unit: DD

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976185_bin.9_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5976186 of study SRP116179.

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.10_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.11_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.15_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.18_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.22_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.24_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Spirochaetales bacterium

characteristic: organism

text: uncultured Spirochaetales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.25_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.28_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Myxococcales bacterium

characteristic: organism

text: uncultured Myxococcales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.29_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.3_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: Lactobacillus johnsonii

characteristic: organism

text: Metagenomic Analysis of Colonic Microbiome of Landrace Finishing Pigs with High and Low Feed Efficiency

text: Lactobacillus johnsonii

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.32_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Prevotella copri

characteristic: project name

characteristic: sample derived from

text: Prevotella copri

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.34_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.45_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5976186 of study SRP116179.

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Mediterranea sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Mediterranea sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.49_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.50_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroides sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroides sp.

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.52_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Treponema sp.

characteristic: organism

text: uncultured Treponema sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.54_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Ruminococcus flavefaciens

characteristic: project name

characteristic: sample derived from

text: Ruminococcus flavefaciens

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976186_bin.7_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Eubacterium sp.

characteristic: organism

text: uncultured Eubacterium sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976186_bin.9_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5976187 of study SRP116179.

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.10_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.14_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.17_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: Metagenomic Analysis of Colonic Microbiome of Landrace Finishing Pigs with High and Low Feed Efficiency

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.2_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.21_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.29_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.32_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.34_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.38_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.41_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Candidatus Methanomethylophilus alvus

characteristic: project name

characteristic: sample derived from

text: Candidatus Methanomethylophilus alvus

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.42_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.43_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.48_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: common name

characteristic: completeness score

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5976187 of study SRP116179.

text: Digesta

text: China

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Colon

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 2500

text: multi-marker approach

text: Metagenome-assembled genome: SRR5976187_bin.5_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Digesta

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 2500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5976187_bin.8_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University Hospital Basel

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Clinical Virology, University Hospital Basel, Basel, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Christian Beisel, Sarah Nadeau, Chaoran Chen, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler, Tim Roloff, Fanny Wegner, Hans Hirsch, Adrian Egli

characteristic: collector name

text: Switzerland

text: BS

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: active surveillance not initiated by an outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: GISAID Accession ID

text: University Hospital Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Felix-Platter Spital, Basel, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: title


text: University Hospital Basel

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: BL

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:West of Scotland Specialist Virology Centre, NHSGGC / MRC-University of Glasgow Centre for Virus Research

text: COVID-19 Genomics UK Consortium

text: not provided

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: Scotland

text: not provided

text: not provided

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: organism

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


text: IZSLER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Illumina Miseq genome sequencing of a Chlamydia pecorum isolate from a chamois (PV7855).

characteristic: description

text: Italy

characteristic: geographic location (country and/or sea)

text: diseased

text: Rupicapra rupicapra

text: lung

characteristic: isolation source

text: 46�13'12''N 10�51'36''E

characteristic: lat lon

text: Chlamydia pecorum

text: Chlamydia pecorum

characteristic: serovar

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIBE-IG

text: public

text: horse

characteristic: common name

text: The samples RNA were pooled from a asthmatic Selle Fran�ais and health Swiss Warmblood to prepare libraries for PacBio Iso-Seq sequencing. Both were mares.

text: Equus caballus

characteristic: organism

text: Equus caballus

text: SF30_SW91 -Mare


text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Technical University of Munich

text: freshwater biome

characteristic: broad-scale environmental context

unit: m

characteristic: depth

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lake

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: River

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Stream

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 33_Seinbruchgewaesser_Kleine_Lummersbach

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Small water body

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Small water body

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 64_Altarm_des_Dortmund_Ems_Kanals_in_Bevergern

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Lake

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 103_Brentanosee_in_Darmstadt_Kranichstein

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lake

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

text: 145_Grosser_Tonteich_in_Koerbis_Krug_Bestensee

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Lake

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 223_Wetter_bei_Butzbach_Gewaesserkennziffer_248453

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Stream

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

text: Small water body

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: not collected

text: not collected

text: River

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 292_Kindermannsee_Park_Babelsberg_in_Potsdam

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Lake

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 303_Dortmund_Ems_Seitenkanal_Kanalhaltung_5

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lake

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

text: 335_Graben_23_mit_Einfluss_in_den_Neuklostersee_in_23992_Nakenstorf

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Stream

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 336_Graben_23_mit_Einfluss_in_den_Neuklostersee_in_23992_Nakenstorf

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Lake

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 347_Grenzbach_Dommitzsch_Unterhalb_Bahnlinie_Torgau_Dommitzsch

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Stream

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

text: 390_Sorpetalsperre_Steg_der_oekolog_Station

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Lake

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 394_Ententeich_Regnewasserrueckhaltebecken

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Small water body

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Lake

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: River

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Stream

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 405_Erpe_Fliessgewaesser_in_34471_Ehringen

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Stream

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Small water body

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 517_Abgrabungssee_noerdl_von_Altlandsberg

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Lake

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 537_Mergelgrube_Weide_NSG_Halbinsel_Devin

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Small water body

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

text: 605_Hoheheideteich_an_Kuhlake_in_Spandauer_Forst

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Small water body

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 618_Untersee_im_Gartenschaupark_Rietberg

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Lake

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 707_Teich_in_der_Feldflur_Gemarkung_Wuenschendorf_

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Small water body

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Technical University of Munich

text: public

characteristic: SRA accession

text: 700_770_Horster_Grenzgraben_Lit_Entwaesserungsgraben

characteristic: Submitter Id

text: freshwater biome

characteristic: collection date

text: freshwater sediment

characteristic: description

text: sediment

text: Germany

characteristic: geographic location (country and/or sea)

text: Stream

text: sediment metagenome

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq

characteristic: title


characteristic: External Id

text: Technical University of Munich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 701_771_Horster_Grenzgraben_zw_Lit_Feld_und_Dunkelfeld_2_Referenz

text: freshwater biome

characteristic: broad-scale environmental context

characteristic: depth

text: freshwater sediment

text: sediment

text: Germany

text: Stream

text: sediment metagenome

characteristic: organism

text: Tatort Gewaesser

text: sediment metagenome

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: Technical University of Munich

text: public

text: 702_772_Horster_Grenzgraben_Entwaesserungsgraben

text: freshwater biome

text: freshwater sediment

characteristic: elevation

text: sediment

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Stream

characteristic: local environmental context

text: sediment metagenome

text: Tatort Gewaesser

characteristic: project name

text: sediment metagenome

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: West of Scotland Specialist Virology Centre, NHSGGC / MRC-University of Glasgow Centre for Virus Research

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

characteristic: title

text: not provided

characteristic: virus identifier


E-MTAB-11957:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Inner Mongolia Medical University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11957:Sample 1

tag: Namespace:Inner Mongolia Medical University

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: diabetes mellitus type 2 associated cataract

characteristic: clinical information

text: human

characteristic: common name

text: Protocols: Fresh feces of volunteers were collected, frozen and stored in -80℃ for a few months. The total DNA from every fecal sample was extracted by Magnetic Soil And Stool DNA Kit of TIANGEN BIOTECH (BEIJING) CO., LTD, then constructed into a library and sequenced by Illumina PE150. This process was completed by the Wuhan metware Biotechnology Co., Ltd.There are mainly two methods in QC for DNA samples:(1) DNA degradation degree and potential contamination was monitored on 1%agarose gels. (2) DNA concentration was measured using Qubit® dsDNA Assay Kit inQubit® 2.0 Flurometer (Life Technologies, CA, USA). OD value is between 1.8~2.0,DNA contents above 1ug are used to constructlibrary. A total amount of 1μg DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB,USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350bp,then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analysed for size distribution by Agilent2100 Bioanalyzer and quantified using real-timePCR.

characteristic: description

text: type 2 diabetes mellitus

characteristic: disease

characteristic: disease staging

text: Mongolian

characteristic: ethnic group

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: faeces

characteristic: organism substance

text: Homo sapiens

text: Sample 1


E-MTAB-11957:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 10

text: ArrayExpress

text: human

text: pre-diabetes

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 10

characteristic: title


E-MTAB-11957:Sample 11

characteristic: External Id

text: Inner Mongolia Medical University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11957:Sample 11

text: ArrayExpress

text: human

text: pre-diabetes

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 11


E-MTAB-11957:Sample 12

text: Inner Mongolia Medical University

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11957:Sample 12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: pre-diabetes

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 12


E-MTAB-11957:Sample 13

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 13

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: normal

characteristic: disease

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 13


E-MTAB-11957:Sample 14

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 14

text: ArrayExpress

text: human

text: normal

characteristic: disease staging

text: Mongolian

characteristic: ethnic group

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 14


E-MTAB-11957:Sample 15

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 15

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

characteristic: organism substance

text: Homo sapiens

text: Sample 15


E-MTAB-11957:Sample 16

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 16

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 16

characteristic: title


E-MTAB-11957:Sample 17

text: Inner Mongolia Medical University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11957:Sample 17

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 17


E-MTAB-11957:Sample 18

text: Inner Mongolia Medical University

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11957:Sample 18

characteristic: Submitter Id

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 18


E-MTAB-11957:Sample 19

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 19

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 19


E-MTAB-11957:Sample 2

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 2

text: ArrayExpress

text: human

text: type 2 diabetes mellitus

characteristic: disease

characteristic: disease staging

text: Mongolian

characteristic: ethnic group

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 2


E-MTAB-11957:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 20

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 20


E-MTAB-11957:Sample 21

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 21

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

characteristic: organism substance

text: Homo sapiens

text: Sample 21


E-MTAB-11957:Sample 22

text: Inner Mongolia Medical University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11957:Sample 22

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 22

characteristic: title


E-MTAB-11957:Sample 23

text: Inner Mongolia Medical University

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11957:Sample 23

text: ArrayExpress

text: human

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 23


E-MTAB-11957:Sample 24

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 24

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: normal

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 24


E-MTAB-11957:Sample 3

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 3

text: ArrayExpress

text: human

characteristic: description

text: type 2 diabetes mellitus

characteristic: disease

characteristic: disease staging

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 3


E-MTAB-11957:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 4

text: ArrayExpress

text: human

text: type 2 diabetes mellitus

text: Mongolian

characteristic: ethnic group

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 4


E-MTAB-11957:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 5

text: ArrayExpress

text: human

text: type 2 diabetes mellitus

text: Mongolian

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 5


E-MTAB-11957:Sample 6

text: Inner Mongolia Medical University

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11957:Sample 6

text: ArrayExpress

text: human

text: type 2 diabetes mellitus

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

characteristic: organism substance

text: Homo sapiens

text: Sample 6


E-MTAB-11957:Sample 7

text: Inner Mongolia Medical University

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11957:Sample 7

text: ArrayExpress

text: human

text: pre-diabetes

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 7

characteristic: title


E-MTAB-11957:Sample 8

text: Inner Mongolia Medical University

text: public

characteristic: SRA accession

text: E-MTAB-11957:Sample 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: pre-diabetes

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 8


E-MTAB-11957:Sample 9

text: Inner Mongolia Medical University

text: public

text: E-MTAB-11957:Sample 9

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: pre-diabetes

characteristic: disease

text: Mongolian

text: Homo sapiens

text: Homo sapiens

text: faeces

text: Homo sapiens

text: Sample 9


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: COVID-19 Genomics UK Consortium

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


E-MTAB-11953:Spheroids (304)

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11953:Spheroids (304)

tag: Namespace:Department of Cell and Developmental Biology, Department of Internal Medicine, Department of Biomedical Engineering Medicine, University of Michigan Medical School, Ann Arbor, MI

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: iPSC17 WT 7B2

characteristic: cell line

text: induced pluripotent stem cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: NKX2-1-optimized Spheroid Differentiation Protocol: Generation of definitive endoderm (DE) from hPSCs (differentiation days 1 - 3) was carried out as previously described with slight modifications (D’Amour et al., 2006; McCracken et al., 2011; Spence et al., 2011; Dye et al., 2015). Briefly, 100 ng/mL ActivinA (R&D Systems, Cat#338-AC) was added in RPMI 1640 media (Thermo Fisher, Cat#21875034) with increasing concentrations of HyClone defined FBS (dFBS) (Fisher Scientific, Cat#SH3007002) on subsequent days (0% day 1, 0.2% day 2, 2% day 3). 50 ng/mL BMP4 (R&D Systems, Cat#314-BP) was added on day 1. Following DE specification, anterior foregut spheroids were generated (differentiation days 4 - 6) by a 3-day treatment with 500 ng/mL FGF4 (lab purified, see supplemental methods), 200 ng/mL NOGGIN (R&D Systems, Cat#6057-NG), and 2 µM CHIR99021 (APExBIO, Cat#A3011). 2 µM all trans retinoic acid (Sigma, Cat#R2625) was added on the final day of anterior foregut spheroid generation (day 6). On day 7, self-organizing 3D spheroids that had detached from the tissue culture dish were collected with a P200 pipette and were transferred into Matrigel (Corning, Cat#354234) as previously described (McCracken et al., 2011; Miller et al., 2019). After the Matrigel had solidified, encapsulating spheroids, they were cultured in media that contained 250 ng/mL WNT3a (R&D Systems, Cat#5036-WN), 500 ng/mL RSPO1 (lab purified, see supplemental methods), and 10 ng/mL BMP4 for 3 days to induce NKX2-1+ cells (differentiation days 7 - 9). Following DE specification (after day 3), RPMI 1640 media + 2% dFBS was used on all days to dilute growth factors. Fresh media and growth factors were added each day. All tubes and pipette tips were pre-washed in 1X HBSS with 1% BSA to prevent cell adhesion to the plastic. 3D cultures (spheroids, LPOs, iBTOs) were removed from Matrigel using a P1000 pipette tip and vigorously pipetted in a 1.5 mL Eppendorf tube to remove as much Matrigel as possible. Tissue was centrifuged at 300g for 3 minutes at 4ºC, then excess media and Matrigel was removed. Tissue was digested to single cells using 0.5 mL TrypLE (Invitrogen, Cat#12605010) and incubated at 37ºC for 30 minutes, adding mechanical digestion with pipette every 10 minutes. After 30 minutes, trypsinization was quenched with 1X HBSS + 1% BSA. Cells were passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040) and centrifuged at 300g for 3 minutes at 4ºC. Cells were resuspended in 1 mL 1X HBSS + 1% BSA and counted using a hemocytometer, centrifuged at 300g for 3 minutes at 4ºC, and resuspended to a final concentration of 1,100 cells/µL. If samples were planned for combined submission, cells would be cryopreserved in CryoStor CS10 (Biolife Solutions, Cat#210102). If cryopreservation was used, cold CryoStor CS10 solution was added to the cells and mixed thoroughly and transferred to a cryovial. The cells were incubated at 2 – 8ºC for 10 minutes, then cryopreserved in using an isopropanol freezing container for 24 hours, then transferred to liquid nitrogen. Cells were thawed in a 37ºC water bath, washed in DMEM/F12 (Corning, Cat#10-092-CV) with 10% FBS, centrifuged at 300g for 5 minutes,and supernatant was removed. The pellet was then washed in HBSS + 1% BSA, then resuspended in 1 mL fresh HBSS + 1% BSA, passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040), and counted using a hemocytometer. Cells were centrifuged at 300g for 3 minutes at 4ºC and resuspended to a final concentration of 1,100 cells/µL. Approximately 100,000 fresh or thawed cells were put on ice and single cell libraries were immediately prepared at the 10X Chromium at the University of Michigan Sequencing Core with a target of 10,000 cells per sample. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used

characteristic: description

text: spheroid differentiation media

characteristic: growth condition

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: progenitor cell type

text: Homo sapiens

text: Spheroids (304)

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Cell and Developmental Biology, Department of Internal Medicine, Department of Biomedical Engineering Medicine, University of Michigan Medical School, Ann Arbor, MI

text: public

text: E-MTAB-11953:3-week LPO Batch#1 (4511-1)

text: ArrayExpress

text: iPSC17 WT 7B2

text: lung progenitor cell

text: human

text: Protocols: Growth and Maintenance of LPOs: NKX2-1-optimized spheroids were transferred to “3 Factor” (3F) bud tip maintenance media as previously described (Miller et al., 2018, 2019), including 50 nM all trans retinoic acid (Sigma, Cat#R2625), 10 ng/mL FGF7 (R&D Systems, Cat#251-KG), and 3 µM CHIR99021(APExBIO, Cat#A3011) in serum-free basal media. Serum-free basal media consists of DMEM/F12 containing HEPES and L-Glutamine (Corning, Cat#10-092-CV), 100 U/mL penicillin-streptomycin (Thermo Fisher, Cat#15140122), 1x B-27 supplement (Thermo Fisher, Cat#17504044), 1x N-2 supplement (Thermo Fisher, Cat#17502048), 0.05% BSA (Sigma, Cat#A9647), 50 µg/mL L-ascorbic acid (Sigma, Cat#A4544), and 0.4 µM 1-Thioglycerol (Sigma, Cat#M1753). LPOs were grown for 3 weeks, then whole passaged or needle or pipette sheared every 2 - 4 weeks. Whole passaging was achieved by collecting LPOs into a 1.5mL microcentrifuge tube and gently releasing them from Matrigel by a P1000 cut pipette tip (P200 tip was used for young/small LPOs). LPOs were spun in a microcentrifuge tube, residual media and Matrigel was removed, then LPOs were re-suspended in Matrigel (Corning, Cat#354234) with a P1000 cut pipette tip. ~35 µL droplets of Matrigel were placed into the center of wells of a 24-well tissue culture plate (Thermo Fisher, Cat#12565163), and the plate was inverted and placed in an incubator at 37ºC for 20 minutes. Whole passaging was performed approximately every 2 – 3 weeks at a ratio of 1:2 (individual LPOs were kept whole but each were given more space, i.e., n spheroids were given 2x the space to grow) for up to 17-weeks. For LPOs passaged by needle or pipette shearing, LPOs were passed through a 27-gauge needle or P200 pipette, respectively, and embedded in fresh Matrigel as previously described (Miller et al., 2018, 2019, 2020). LPOs were fed with 3F media every 2 - 4 days. All tubes and pipette tips were pre-washed in 1X HBSS with 1% BSA to prevent cell adhesion to the plastic. 3D cultures (spheroids, LPOs, iBTOs) were removed from Matrigel using a P1000 pipette tip and vigorously pipetted in a 1.5 mL Eppendorf tube to remove as much Matrigel as possible. Tissue was centrifuged at 300g for 3 minutes at 4ºC, then excess media and Matrigel was removed. Tissue was digested to single cells using 0.5 mL TrypLE (Invitrogen, Cat#12605010) and incubated at 37ºC for 30 minutes, adding mechanical digestion with pipette every 10 minutes. After 30 minutes, trypsinization was quenched with 1X HBSS + 1% BSA. Cells were passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040) and centrifuged at 300g for 3 minutes at 4ºC. Cells were resuspended in 1 mL 1X HBSS + 1% BSA and counted using a hemocytometer, centrifuged at 300g for 3 minutes at 4ºC, and resuspended to a final concentration of 1,100 cells/µL. If samples were planned for combined submission, cells would be cryopreserved in CryoStor CS10 (Biolife Solutions, Cat#210102). If cryopreservation was used, cold CryoStor CS10 solution was added to the cells and mixed thoroughly and transferred to a cryovial. The cells were incubated at 2 – 8ºC for 10 minutes, then cryopreserved in using an isopropanol freezing container for 24 hours, then transferred to liquid nitrogen. Cells were thawed in a 37ºC water bath, washed in DMEM/F12 (Corning, Cat#10-092-CV) with 10% FBS, centrifuged at 300g for 5 minutes,and supernatant was removed. The pellet was then washed in HBSS + 1% BSA, then resuspended in 1 mL fresh HBSS + 1% BSA, passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040), and counted using a hemocytometer. Cells were centrifuged at 300g for 3 minutes at 4ºC and resuspended to a final concentration of 1,100 cells/µL. Approximately 100,000 fresh or thawed cells were put on ice and single cell libraries were immediately prepared at the 10X Chromium at the University of Michigan Sequencing Core with a target of 10,000 cells per sample. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used

text: lung progenitor organoid culture

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

text: 3-week LPO Batch#1 (4511-1)


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11953:3-week LPO Batch#2 (4511-2)

text: ArrayExpress

text: iPSC17 WT 7B2

text: lung progenitor cell

text: human

text: lung progenitor organoid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

text: 3-week LPO Batch#2 (4511-2)


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11953:6-week LPO Batch#1 (4511-3)

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: iPSC17 WT 7B2

text: lung progenitor cell

text: human

text: lung progenitor organoid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

text: 6-week LPO Batch#1 (4511-3)


text: public

text: E-MTAB-11953:6-week LPO Batch#2 (4511-4)

text: ArrayExpress

text: iPSC17 WT 7B2

characteristic: cell line

text: lung progenitor cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: lung progenitor organoid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: induced pluripotent stem cell

text: Homo sapiens

text: 6-week LPO Batch#2 (4511-4)


E-MTAB-11953:10-week LPO (4511-5)

text: public

text: E-MTAB-11953:10-week LPO (4511-5)

text: ArrayExpress

text: iPSC17 WT 7B2

text: lung progenitor cell

text: human

text: lung progenitor organoid culture

characteristic: growth condition

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: 10-week LPO (4511-5)

characteristic: title


E-MTAB-11953:4-week iBTO (5178-2)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11953:4-week iBTO (5178-2)

text: ArrayExpress

text: iPSC17 WT 7B2

text: induced bud tip progenitor cell

text: human

text: Protocols: Growth and Maintenance of iBTOs: iBTPs in LPOs grown in 3F media for 3 - 17 weeks were isolated by FACS (see below). After collection, iBTPs were centrifuged at 300g for 5 minutes at 4ºC and media was removed. Matrigel (Corning, Cat#354234) was added to the cells at a concentration of 5,000 cells/µL Matrigel and 3 - 20 µL droplets of Matrigel were placed into the center of wells of a 24-well tissue culture plate (Thermo Fisher, Cat#12565163). The plate was inverted and placed in an incubator at 37ºC for 20 minutes. iBTPs were fed every 2 - 4 days with 3F media and allowed to reform organoids (iBTOs) for up to 4 weeks. iBTOs were whole passaged as described above every 2 - 3 weeks following organoid formation. All tubes and pipette tips were pre-washed in 1X HBSS with 1% BSA to prevent cell adhesion to the plastic. 3D cultures (spheroids, LPOs, iBTOs) were removed from Matrigel using a P1000 pipette tip and vigorously pipetted in a 1.5 mL Eppendorf tube to remove as much Matrigel as possible. Tissue was centrifuged at 300g for 3 minutes at 4ºC, then excess media and Matrigel was removed. Tissue was digested to single cells using 0.5 mL TrypLE (Invitrogen, Cat#12605010) and incubated at 37ºC for 30 minutes, adding mechanical digestion with pipette every 10 minutes. After 30 minutes, trypsinization was quenched with 1X HBSS + 1% BSA. Cells were passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040) and centrifuged at 300g for 3 minutes at 4ºC. Cells were resuspended in 1 mL 1X HBSS + 1% BSA and counted using a hemocytometer, centrifuged at 300g for 3 minutes at 4ºC, and resuspended to a final concentration of 1,100 cells/µL. If samples were planned for combined submission, cells would be cryopreserved in CryoStor CS10 (Biolife Solutions, Cat#210102). If cryopreservation was used, cold CryoStor CS10 solution was added to the cells and mixed thoroughly and transferred to a cryovial. The cells were incubated at 2 – 8ºC for 10 minutes, then cryopreserved in using an isopropanol freezing container for 24 hours, then transferred to liquid nitrogen. Cells were thawed in a 37ºC water bath, washed in DMEM/F12 (Corning, Cat#10-092-CV) with 10% FBS, centrifuged at 300g for 5 minutes,and supernatant was removed. The pellet was then washed in HBSS + 1% BSA, then resuspended in 1 mL fresh HBSS + 1% BSA, passed through a 40 µm filter (Bel-Art Flowmi, Cat#136800040), and counted using a hemocytometer. Cells were centrifuged at 300g for 3 minutes at 4ºC and resuspended to a final concentration of 1,100 cells/µL. Approximately 100,000 fresh or thawed cells were put on ice and single cell libraries were immediately prepared at the 10X Chromium at the University of Michigan Sequencing Core with a target of 10,000 cells per sample. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 5,000 cells. Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 was used

text: lung progenitor organoid culture

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: lung progenitor cell

text: Homo sapiens

text: 4-week iBTO (5178-2)


E-MTAB-11953:10-week iBTO (5178-1)

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11953:10-week iBTO (5178-1)

text: ArrayExpress

text: iPSC17 WT 7B2

text: induced bud tip progenitor cell

text: human

text: lung progenitor organoid culture

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: lung progenitor cell

text: Homo sapiens

text: 10-week iBTO (5178-1)


text: EMG

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR5240725 of study SRP099123.

text: Feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

characteristic: isolation source

text: Colon

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

text: We studied the intestinal microbiome in feces from sows, five days after farrowing, their piglets during suckling and two weeks after weaning, 5-day old artificially reared and formula-fed siblings, and FP infected with Clostridium difficile.

characteristic: project name

characteristic: sample derived from

text: NextSeq 500

characteristic: sequencing method

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR5240725_bin.10_metaWRAP_v1.3_MAG