E-MTAB-11699:Sample 135

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E-MTAB-11699:Sample 136

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text: Protocols: Cell sample collections. Skeletal muscle and subcutaneous fat samples were collected from Angus-crossbred steers slaughtered at the Virginia Tech Meat Center. Satellite cells were isolated from skeletal muscle by pronase digestion as described before (Leng et al. 2019). The isolated satellite cells were activated to proliferate as myoblasts by culturing in growth medium composed of Dulbecco’s Modified Eagle Medium (DMEM), 10% fetal bovine serum (FBS), and 1% antibiotics-antimycotics. To induce myoblasts to differentiate into myocytes, myoblasts cultured in growth medium were switched to differentiation medium composed of DMEM and 2% horse serum for 2 days. Preadipocytes from subcutaneous fat were isolated by collagenase digestion as previously described (Hausman et al. 2008). To induce preadipocytes to differentiate into adipocytes, preadipocytes were initially cultured in growth medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics) to reach confluency, then in induction medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics, 10 μg/mL insulin, 1 μM dexamethasone, 0.5 mM isobutyl methylxanthine, and 200 μM indomethacin) for 2 days, and lastly in maintenance medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics, 1 μg/mL insulin) for 10 days.Adult tissue collections. Procedures for tissue collection followed the Animal Care and Use protocol (#18464) approved by the Institutional Animal Care and Use Committee (IACUC), University of California, Davis (UCD). Four cattle (2 males and 2 females) were slaughtered at UCD using captive bolt under USDA inspection at 14 months old and were intact male and female Line 1 Herefords that had the same sire, provided by Fort Keogh Livestock and Range Research Lab (Tixier-Boichard et al. 2021). Tissue samples were flash frozen in liquid nitrogen then stored at -80 °C until further assay processing. Fetal tissue collections. Fetal sample collection and tissue collection were approved by the Institutional Animal Care and Use Committee (IACUC), University of Idaho (2017-67). Four pregnant females Line 1 Herefords were slaughtered at UI meats lab using captive bolt under USDA inspection. Animals were provided by Fort Keogh Livestock and Range Research Lab (Tixier-Boichard et al. 2021). Tissue samples were flash frozen in liquid nitrogen then stored at -80 °C until further assay processing. Cell sample collections. Skeletal muscle and subcutaneous fat samples were collected from Angus-crossbred steers slaughtered at the Virginia Tech Meat Center. Satellite cells were isolated from skeletal muscle by pronase digestion as described before (Leng et al. 2019). The isolated satellite cells were activated to proliferate as myoblasts by culturing in growth medium composed of Dulbecco’s Modified Eagle Medium (DMEM), 10% fetal bovine serum (FBS), and 1% antibiotics-antimycotics. To induce myoblasts to differentiate into myocytes, myoblasts cultured in growth medium were switched to differentiation medium composed of DMEM and 2% horse serum for 2 days. Preadipocytes from subcutaneous fat were isolated by collagenase digestion as previously described (Hausman et al. 2008). To induce preadipocytes to differentiate into adipocytes, preadipocytes were initially cultured in growth medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics) to reach confluency, then in induction medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics, 10 μg/mL insulin, 1 μM dexamethasone, 0.5 mM isobutyl methylxanthine, and 200 μM indomethacin) for 2 days, and lastly in maintenance medium (DMEM/F12, 10% FBS, 1% antibiotics-antimycotics, 1 μg/mL insulin) for 10 days. Adult tissue collections. Procedures for tissue collection followed the Animal Care and Use protocol (#18464) approved by the Institutional Animal Care and Use Committee (IACUC), University of California, Davis (UCD). Four cattle (2 males and 2 females) were slaughtered at UCD using captive bolt under USDA inspection at 14 months old and were intact male and female Line 1 Herefords that had the same sire, provided by Fort Keogh Livestock and Range Research Lab (Tixier-Boichard et al. 2021). Tissue samples were flash frozen in liquid nitrogen then stored at -80 °C until further assay processing. Fetal tissue collections. Fetal sample collection and tissue collection were approved by the Institutional Animal Care and Use Committee (IACUC), University of Idaho (2017-67). Four pregnant females Line 1 Herefords were slaughtered at UI meats lab using captive bolt under USDA inspection. Animals were provided by Fort Keogh Livestock and Range Research Lab (Tixier-Boichard et al. 2021). Tissue samples were flash frozen in liquid nitrogen then stored at -80 °C until further assay processing. RNA-seq library construction. Tissue samples (Additional file18) were collected from storage at -80oC and ground to a powder using a mortar and pestle and liquid nitrogen. The tissue was next homogenized in QIAzol Lysis Reagent (Qiagen Catalog No. 79306) using a QIAshredder spin column (Qiagen Catalog No. 79656). After centrifugation, the lysate was mixed with chloroform, shaken vigorously for 15 sec, incubated for 2 - 3 min at room temperature, and centrifuged for 15 min at 12,000 x g at 4°C. The upper, aqueous phase was transferred to a new collection tube and 1.5 vol of 100% ethanol was added and mixed thoroughly by pipetting up and down several times. Total RNA was then isolated from the sample using the RNeasy Mini Kit (Qiagen Catalog No. 74106) according to the manufacturer’s instructions. Contaminating DNA was removed by treating total RNA with DNase (AM1906, Ambion). Total RNA quantity was measured with the Quant-It RiboGreen RNA Assay Kit (Life Technologies Corp., Carlsbad, CA) and quality assessed by fragment analysis (Advance Analytical Technologies, Inc., Ankeny IA). Mammary gland RNA-seq and miRNA-seq library construction. The 16 animals used in this study were Holstein-Friesian Heifers from a single herd managed at the AgResearch Research Station in Ruakura, NZ. All experimental protocols were approved by the AgResearch, NZ, ethics committee, and carried out according to their guidelines. Samples were collected from the same animals at 5 time points: virgin state before pregnancy between 13 and 15 months of age (virgin), mid pregnant at 100 day of pregnancy, late pregnant ~2 weeks pre-calving, early lactation ~2 weeks post-calving, and at peak lactation, 34-38 days post-calving. Tissue samples were obtained by mammary biopsy using the Farr method (Farr et al. 1996). Lactating cows were milked before biopsy and sampled within 5 hours of milking. Biopsy sites were clipped and given aseptic skin preparation (povidone iodine base scrub and iodine tincture) and subcutaneous local anesthetic (4 ml per biopsy site). Core biopsies were taken using a powered sampling cannula (4.5 mm internal diameter) inserted into a 2 cm incision. The resulting samples of mammary gland parenchyma measured 70 mm in length, with a 4 mm diameter. Small slices from each sample were preserved for histology before mammary epithelial organoids were separated from surrounding adipose and connective tissue to allow for secretory-specific signals in the RNA-seq analysis. In preparation for isolating organoids, tissue samples were digested in a freshly prepared collagenase solution containing 0.2% collagenase A (Roche), 0.05% trypsin (1:250 powder, 100U/ml Gibco), hyaluronidase (Sigma), 5% fetal calf serum (Hyclone), Pen/Strep/Fungizone solution (Hyclone) or 5 µg/ml Gentamycin (Sigma) in DMEM/F12 (Gibco) with 10 ng/ml insulin. Samples were minced to a fine slurry and incubated in this freshly prepared collagenase solution (10 ml solution / g tissue) for 3.5 hours at 37°C in a 50 ml conical tube with slow shaking (120 rpm). Digested tissue was centrifuged at 453 xg for 10 min at 4°C, after which the supernatant and fat layers were discarded, and the pellet was gently resuspended in 5 ml DMEM/F12 without serum. A further 5 ml DMEM/F12 without serum was added, and the sample was centrifuged at 453 xg for 10 min at 4°C. The media was discarded, and the pellet was gently resuspended in 10 ml DMEM/F12 and centrifuged for another 10 min at 453 xg and 4°C. The media was discarded, and pellet resuspended in 10 ml DMEM/F12 for a third time, and the sample centrifuged in a series of brief spins achieved by allowing the centrifuge to reach 453 xg for two seconds before applying the brake. These brief pulse spins were repeated at least 4 times, or until examining the sample under a microscope revealed primarily epithelial organoid clusters and very few single cells. At this point, the organoid pellet was resuspended in 1 ml TRIzol and stored at -80°C until RNA isolation. High-quality total RNA (RIN > 7) was extracted from frozen mammary epithelial organoid pellets using NucleoSpin® miRNA isolation kit (MACHEREY-NAGEL) according to the manufacturer’s protocol, isolating large and small (<200 nt) fractions separately. The “large” RNA fraction was used to prepare strand-specific poly-A+ RNA-seq libraries for sequencing. The “small” RNA fraction was used to make miRNA-seq libraries using NEXTflexTM small RNA-seq Kitv3.miRNA-seq library construction. Tissue samples (Additional file18) were collected similar to that described in previous section. QIAseq miRNA Library Kit (Qiagen, cat no. 331505) and QIAseq miRNA NGS 96 Index IL (Qiagen, cat no. 331565) kits were used to isolate miRNAs from all tissues except mammary gland. miRNAs from mammary gland were isolated using NEXTflexTM small RNA-seq Kitv3 (Illumina) according to the manufacturer’s instructions. The isolated miRNA was subjected to 3’ ligation to ligate a preadenylated DNA adaptor to the 3’ends of all miRNAs. An RNA adaptor was then ligated to the 5’ end of the mature miRNA to complete 5’ ligation. cDNA synthesis was completed using a reverse transcriptase (RT) primer containing integrated unique molecular identifiers (UMI). The RT primer bound to the 3’ adaptor region and facilitated conversion of the 3’/5’ ligated miRNAs into cDNA while a UMI was assigned to every miRNA molecule. After reverse transcription, a clean-up of the cDNA was performed using a streamlined magnetic bead-based method. Library amplification was accomplished by a universal forward primer from a plate being paired with 1 of 96 dried reverse primers in the same plate (Qiagen, cat no. 331565) to assign each sample a unique custom index. Following library amplification, a clean-up of the miRNA library was performed using a streamlined magnetic bead-based method. Libraries were then evaluated for quantity and quality measures before being normalized and pooled for Illumina sequencing.

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E-MTAB-11699:Sample 137

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E-MTAB-11699:Sample 139

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E-MTAB-11699:Sample 14

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E-MTAB-11699:Sample 140

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E-MTAB-11699:Sample 141

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E-MTAB-11699:Sample 142

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E-MTAB-11699:Sample 144

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E-MTAB-11699:Sample 145

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E-MTAB-11699:Sample 146

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E-MTAB-11699:Sample 147

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E-MTAB-11699:Sample 148

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E-MTAB-11699:Sample 149

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E-MTAB-11699:Sample 15

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E-MTAB-11699:Sample 150

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E-MTAB-11699:Sample 151

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E-MTAB-11699:Sample 152

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E-MTAB-11699:Sample 153

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E-MTAB-11699:Sample 154

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E-MTAB-11699:Sample 155

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E-MTAB-11699:Sample 156

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E-MTAB-11699:Sample 157

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E-MTAB-11699:Sample 158

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E-MTAB-11699:Sample 159

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E-MTAB-11699:Sample 16

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E-MTAB-11699:Sample 160

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E-MTAB-11699:Sample 161

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E-MTAB-11699:Sample 162

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E-MTAB-11699:Sample 163

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E-MTAB-11699:Sample 164

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E-MTAB-11699:Sample 165

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E-MTAB-11699:Sample 166

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E-MTAB-11699:Sample 167

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text: Sample 167


E-MTAB-11699:Sample 168

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E-MTAB-11699:Sample 169

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text: Sample 169


E-MTAB-11699:Sample 17

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E-MTAB-11699:Sample 170

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text: Sample 170


E-MTAB-11699:Sample 171

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text: Sample 171


E-MTAB-11699:Sample 172

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text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: caput epididymis

text: Bos taurus

text: Sample 172

characteristic: title


E-MTAB-11699:Sample 173

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 173

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: caput epididymis

characteristic: organism part

text: Bos taurus

text: Sample 173


E-MTAB-11699:Sample 174

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 174

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: ovary

text: Bos taurus

text: Sample 174


E-MTAB-11699:Sample 175

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 175

characteristic: Submitter Id

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: virgin mammary gland

text: Bos taurus

text: Sample 175


E-MTAB-11699:Sample 176

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 176

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: uterine endometrium

text: Bos taurus

text: Sample 176


E-MTAB-11699:Sample 177

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 177

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 177

characteristic: title


E-MTAB-11699:Sample 178

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 178

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

characteristic: organism part

text: Bos taurus

text: Sample 178


E-MTAB-11699:Sample 179

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 179

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 179


E-MTAB-11699:Sample 18

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 18

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 18


E-MTAB-11699:Sample 180

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 180

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 180


E-MTAB-11699:Sample 181

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 181

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: virgin mammary gland

text: Bos taurus

text: Sample 181

characteristic: title


E-MTAB-11699:Sample 182

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 182

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: abomasum

characteristic: organism part

text: Bos taurus

text: Sample 182


E-MTAB-11699:Sample 183

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 183

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: abomasum

text: Bos taurus

text: Sample 183


E-MTAB-11699:Sample 184

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 184

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: cerebellum

text: Bos taurus

text: Sample 184


E-MTAB-11699:Sample 185

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 185

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: cerebellum

text: Bos taurus

text: Sample 185


E-MTAB-11699:Sample 186

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 186

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: abomasum

text: Bos taurus

text: Sample 186

characteristic: title


E-MTAB-11699:Sample 187

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 187

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: abomasum

characteristic: organism part

text: Bos taurus

text: Sample 187


E-MTAB-11699:Sample 188

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 188

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: subcutaneous adipose tissue

text: Bos taurus

text: Sample 188


E-MTAB-11699:Sample 189

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 189

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: esophagus

text: Bos taurus

text: Sample 189


E-MTAB-11699:Sample 19

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 19

text: Holstein

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 19


E-MTAB-11699:Sample 190

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 190

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: gal bladder

text: Bos taurus

text: Sample 190

characteristic: title


E-MTAB-11699:Sample 191

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 191

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: kidney

characteristic: organism part

text: Bos taurus

text: Sample 191


E-MTAB-11699:Sample 192

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 192

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 192


E-MTAB-11699:Sample 193

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 193

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 193


E-MTAB-11699:Sample 194

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 194

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 194


E-MTAB-11699:Sample 195

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 195

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: LD muscle

text: Bos taurus

text: Sample 195

characteristic: title


E-MTAB-11699:Sample 196

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 196

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: pituitary

characteristic: organism part

text: Bos taurus

text: Sample 196


E-MTAB-11699:Sample 197

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 197

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pituitary

text: Bos taurus

text: Sample 197


E-MTAB-11699:Sample 198

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 198

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: reticulum

text: Bos taurus

text: Sample 198


E-MTAB-11699:Sample 199

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 199

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: rumen

text: Bos taurus

text: Sample 199


E-MTAB-11699:Sample 2

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 2

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 2

characteristic: title


E-MTAB-11699:Sample 20

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 20

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: gonad

characteristic: organism part

text: Bos taurus

text: Sample 20


E-MTAB-11699:Sample 200

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 200

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: testis

text: Bos taurus

text: Sample 200


E-MTAB-11699:Sample 201

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 201

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: testis

text: Bos taurus

text: Sample 201


E-MTAB-11699:Sample 202

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 202

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: testis

text: Bos taurus

text: Sample 202


E-MTAB-11699:Sample 203

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 203

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: testis

text: Bos taurus

text: Sample 203

characteristic: title


E-MTAB-11699:Sample 204

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 204

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: internal tongue muscle

characteristic: organism part

text: Bos taurus

text: Sample 204


E-MTAB-11699:Sample 205

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 205

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 205


E-MTAB-11699:Sample 206

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 206

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 206


E-MTAB-11699:Sample 207

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 207

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 207


E-MTAB-11699:Sample 208

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 208

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 208

characteristic: title


E-MTAB-11699:Sample 209

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 209

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: myoblast

characteristic: organism part

text: Bos taurus

text: Sample 209


E-MTAB-11699:Sample 21

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 21

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 21


E-MTAB-11699:Sample 210

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 210

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 210


E-MTAB-11699:Sample 211

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 211

text: Holstein

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 211


E-MTAB-11699:Sample 212

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 212

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: myoblast

text: Bos taurus

text: Sample 212

characteristic: title


E-MTAB-11699:Sample 213

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 213

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: myocyte

characteristic: organism part

text: Bos taurus

text: Sample 213


E-MTAB-11699:Sample 214

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 214

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 214


E-MTAB-11699:Sample 215

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 215

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 215


E-MTAB-11699:Sample 216

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 216

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 216


E-MTAB-11699:Sample 217

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 217

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 217

characteristic: title


E-MTAB-11699:Sample 218

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 218

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: myocyte

characteristic: organism part

text: Bos taurus

text: Sample 218


E-MTAB-11699:Sample 219

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 219

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 219


E-MTAB-11699:Sample 22

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 22

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 22


E-MTAB-11699:Sample 220

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 220

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 220


E-MTAB-11699:Sample 221

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 221

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: myocyte

text: Bos taurus

text: Sample 221

characteristic: title


E-MTAB-11699:Sample 222

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 222

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: myocyte

characteristic: organism part

text: Bos taurus

text: Sample 222


E-MTAB-11699:Sample 223

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 223

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 223


E-MTAB-11699:Sample 224

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 224

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 224


E-MTAB-11699:Sample 225

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 225

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 225


E-MTAB-11699:Sample 226

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 226

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: pre-adipocytes

text: Bos taurus

text: Sample 226

characteristic: title


E-MTAB-11699:Sample 227

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 227

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: pre-adipocytes

characteristic: organism part

text: Bos taurus

text: Sample 227


E-MTAB-11699:Sample 23

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 23

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 23


E-MTAB-11699:Sample 24

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 24

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 24


E-MTAB-11699:Sample 25

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 25

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 25


E-MTAB-11699:Sample 26

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 26

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 26

characteristic: title


E-MTAB-11699:Sample 27

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 27

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: muscle

characteristic: organism part

text: Bos taurus

text: Sample 27


E-MTAB-11699:Sample 28

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 28

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: spleen

text: Bos taurus

text: Sample 28


E-MTAB-11699:Sample 29

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 29

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: spleen

text: Bos taurus

text: Sample 29


E-MTAB-11699:Sample 3

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 3

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 3


E-MTAB-11699:Sample 30

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 30

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: umbilical cord

text: Bos taurus

text: Sample 30

characteristic: title


E-MTAB-11699:Sample 31

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 31

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: umbilical cord

characteristic: organism part

text: Bos taurus

text: Sample 31


E-MTAB-11699:Sample 32

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 32

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 32


E-MTAB-11699:Sample 33

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 33

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 33


E-MTAB-11699:Sample 34

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 34

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 34


E-MTAB-11699:Sample 35

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 35

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: mammary gland

text: Bos taurus

text: Sample 35

characteristic: title


E-MTAB-11699:Sample 36

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 36

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: mammary gland

characteristic: organism part

text: Bos taurus

text: Sample 36


E-MTAB-11699:Sample 37

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 37

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 37


E-MTAB-11699:Sample 38

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 38

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 38


E-MTAB-11699:Sample 39

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 39

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: gonad

text: Bos taurus

text: Sample 39


E-MTAB-11699:Sample 4

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 4

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: gonad

text: Bos taurus

text: Sample 4

characteristic: title


E-MTAB-11699:Sample 40

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 40

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: gonad

characteristic: organism part

text: Bos taurus

text: Sample 40


E-MTAB-11699:Sample 41

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 41

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 41


E-MTAB-11699:Sample 42

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 42

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 42


E-MTAB-11699:Sample 43

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 43

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 43


E-MTAB-11699:Sample 44

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 44

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 44

characteristic: title


E-MTAB-11699:Sample 45

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 45

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: liver

characteristic: organism part

text: Bos taurus

text: Sample 45


E-MTAB-11699:Sample 46

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 46

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 46


E-MTAB-11699:Sample 47

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 47

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 47


E-MTAB-11699:Sample 48

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 48

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 48


E-MTAB-11699:Sample 49

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 49

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 49

characteristic: title


E-MTAB-11699:Sample 5

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 5

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: gonad

characteristic: organism part

text: Bos taurus

text: Sample 5


E-MTAB-11699:Sample 50

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 50

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 50


E-MTAB-11699:Sample 51

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 51

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 51


E-MTAB-11699:Sample 52

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 52

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 52


E-MTAB-11699:Sample 53

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 53

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: spleen

text: Bos taurus

text: Sample 53

characteristic: title


E-MTAB-11699:Sample 54

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 54

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: umbilical cord

characteristic: organism part

text: Bos taurus

text: Sample 54


E-MTAB-11699:Sample 55

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 55

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: umbilical cord

text: Bos taurus

text: Sample 55


E-MTAB-11699:Sample 56

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 56

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 56


E-MTAB-11699:Sample 57

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 57

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 57


E-MTAB-11699:Sample 58

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 58

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: placental cotyledon

text: Bos taurus

text: Sample 58

characteristic: title


E-MTAB-11699:Sample 59

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 59

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: placental cotyledon

characteristic: organism part

text: Bos taurus

text: Sample 59


E-MTAB-11699:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 6

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 6


E-MTAB-11699:Sample 60

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 60

characteristic: Submitter Id

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: early-lactating mammary gland

text: Bos taurus

text: Sample 60


E-MTAB-11699:Sample 61

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 61

text: Holstein

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: late-pregnant mammary gland

text: Bos taurus

text: Sample 61


E-MTAB-11699:Sample 62

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 62

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: mid-pregnant mammary gland

text: Bos taurus

text: Sample 62

characteristic: title


E-MTAB-11699:Sample 63

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 63

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: mammary gland

characteristic: organism part

text: Bos taurus

text: Sample 63


E-MTAB-11699:Sample 64

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 64

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: mammary gland

text: Bos taurus

text: Sample 64


E-MTAB-11699:Sample 65

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 65

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 65


E-MTAB-11699:Sample 66

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 66

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: brain

text: Bos taurus

text: Sample 66


E-MTAB-11699:Sample 67

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 67

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: gonad

text: Bos taurus

text: Sample 67

characteristic: title


E-MTAB-11699:Sample 68

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 68

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: gonad

characteristic: organism part

text: Bos taurus

text: Sample 68


E-MTAB-11699:Sample 69

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 69

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 69


E-MTAB-11699:Sample 7

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 7

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 7


E-MTAB-11699:Sample 70

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 70

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: heart

text: Bos taurus

text: Sample 70


E-MTAB-11699:Sample 71

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 71

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 71

characteristic: title


E-MTAB-11699:Sample 72

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 72

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: kidney

characteristic: organism part

text: Bos taurus

text: Sample 72


E-MTAB-11699:Sample 73

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 73

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 73


E-MTAB-11699:Sample 74

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 74

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 74


E-MTAB-11699:Sample 75

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 75

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 75


E-MTAB-11699:Sample 76

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 76

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: muscle

text: Bos taurus

text: Sample 76

characteristic: title


E-MTAB-11699:Sample 77

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 77

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: spleen

characteristic: organism part

text: Bos taurus

text: Sample 77


E-MTAB-11699:Sample 78

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 78

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: spleen

text: Bos taurus

text: Sample 78


E-MTAB-11699:Sample 79

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 79

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: umbilical cord

text: Bos taurus

text: Sample 79


E-MTAB-11699:Sample 8

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 8

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: late embryonic stage

text: normal

text: Bos taurus

text: Bos taurus

text: kidney

text: Bos taurus

text: Sample 8


E-MTAB-11699:Sample 80

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 80

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: umbilical cord

text: Bos taurus

text: Sample 80

characteristic: title


E-MTAB-11699:Sample 81

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 81

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: placetal cranucle

characteristic: organism part

text: Bos taurus

text: Sample 81


E-MTAB-11699:Sample 82

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 82

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placetal cranucle

text: Bos taurus

text: Sample 82


E-MTAB-11699:Sample 83

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 83

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placental cotyledon

text: Bos taurus

text: Sample 83


E-MTAB-11699:Sample 84

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 84

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: placental cotyledon

text: Bos taurus

text: Sample 84


E-MTAB-11699:Sample 85

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 85

text: Holstein

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: early-lactating mammary gland

text: Bos taurus

text: Sample 85

characteristic: title


E-MTAB-11699:Sample 86

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 86

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: late-pregnant mammary gland

characteristic: organism part

text: Bos taurus

text: Sample 86


E-MTAB-11699:Sample 87

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 87

text: Holstein

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: mid-pregnant mammary gland

text: Bos taurus

text: Sample 87


E-MTAB-11699:Sample 88

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 88

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: adipocyte

text: Bos taurus

text: Sample 88


E-MTAB-11699:Sample 89

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 89

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: adipocyte

text: Bos taurus

text: Sample 89


E-MTAB-11699:Sample 9

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 9

text: Herford

text: ArrayExpress

text: cattle

text: late embryonic stage

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: liver

text: Bos taurus

text: Sample 9

characteristic: title


E-MTAB-11699:Sample 90

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 90

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: internal tongue muscle

characteristic: organism part

text: Bos taurus

text: Sample 90


E-MTAB-11699:Sample 91

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 91

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: reticulum

text: Bos taurus

text: Sample 91


E-MTAB-11699:Sample 92

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 92

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: reticulum

text: Bos taurus

text: Sample 92


E-MTAB-11699:Sample 93

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 93

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: rumen

text: Bos taurus

text: Sample 93


E-MTAB-11699:Sample 94

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 94

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: rumen

text: Bos taurus

text: Sample 94

characteristic: title


E-MTAB-11699:Sample 95

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 95

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

characteristic: individual

text: Bos taurus

text: Bos taurus

text: omasum

characteristic: organism part

text: Bos taurus

text: Sample 95


E-MTAB-11699:Sample 96

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOWA STATE UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11699:Sample 96

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: abomasum

text: Bos taurus

text: Sample 96


E-MTAB-11699:Sample 97

text: IOWA STATE UNIVERSITY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11699:Sample 97

characteristic: Submitter Id

text: Herford

text: ArrayExpress

text: cattle

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: abomasum

text: Bos taurus

text: Sample 97


E-MTAB-11699:Sample 98

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 98

text: Herford

characteristic: breed

text: ArrayExpress

characteristic: broker name

text: cattle

characteristic: common name

characteristic: description

text: adult

text: normal

text: Bos taurus

text: Bos taurus

text: duodenum

text: Bos taurus

text: Sample 98


E-MTAB-11699:Sample 99

text: IOWA STATE UNIVERSITY

text: public

text: E-MTAB-11699:Sample 99

text: Herford

text: ArrayExpress

text: cattle

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Bos taurus

characteristic: organism

text: Bos taurus

text: duodenum

text: Bos taurus

text: Sample 99

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11313:37_Combo_ERO1_KO_S15_R1_001

tag: Namespace:Istituto di Ricerche Farmacologiche Mario Negri IRCCS

text: ArrayExpress

text: human

text: Protocols: Genetic modification, ERO1-Lα CRISPR-Cas9 Knock-out MDAMB231 metastatic breast tumors. Collection of 5-10 million cells in PDS, and resuspension of the pellet in a buffer. Paclitaxel was injected intravenously (i.v.) at the dose of 15 mg/kg, Q7x2 (every seven days for 2 weeks) and stopped two weeks before the end of the experiment. ISRIB (trans-isomer, Aurogene s.r.l) was dissolved in 10% DMSO (Sigma-Aldrich) in corn oil. It was injected interaperitoneally (i.p.), at the dose 2.5 mg/kg every two days for three weeks. Forty-eight hours after the last dose of ISRIB, mice were sacrificed, and primary breast tumors were isolated and RNA extracted. Protocol: miRNeasy Mini Kit TruSeq Stranded Total RNA Sample Preparation Guide

text: breast cancer

text: ERO1 knockout

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Istituto di Ricerche Farmacologiche Mario Negri IRCCS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11313:38_Combo_ERO1_KO_S16_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11313:39_Combo_ERO1_KO_S15_R1_001

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

text: E-MTAB-11313:40_Combo_ERO1_KO_S16_R1_001

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: breast cancer

characteristic: disease

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11313:32_Isrib_ERO1_KO_S13_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

characteristic: genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11313:33_Isrib_ERO1_KO_S14_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11313:34_Isrib_ERO1_KO_S13_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11313:27_Paclitaxel_ERO1_KO_S11_R1_001

characteristic: Submitter Id

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

text: E-MTAB-11313:28_Paclitaxel_ERO1KO_S12_R1_001

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11313:29_Paclitaxel_ERO1_KO_S11_R1_001

text: ArrayExpress

text: human

text: breast cancer

characteristic: disease

text: ERO1 knockout

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11313:30_Paclitaxel_ERO1_KO_S12_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11313:10_Paclitaxel_ERO1_WT_S4_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: wild type genotype

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11313:6_Paclitaxel_ERO1_WT_S3_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11313:8_Paclitaxel_ERO1_WT_S4_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


text: public

text: E-MTAB-11313:9_Paclitaxel_ERO1_WT_S3_R1_001

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11313:21_Vehicle_ERO1_KO_S9_R1_001

text: ArrayExpress

text: human

characteristic: description

text: breast cancer

characteristic: disease

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11313:22_Vehicle_ERO1_KO_S10_R1_001

text: ArrayExpress

text: human

text: Protocols: Genetic modification, ERO1-Lα CRISPR-Cas9 Knock-out MDAMB231 metastatic breast tumors. Collection of 5-10 million cells in PDS, and resuspension of the pellet in a buffer. Paclitaxel was injected intravenously (i.v.) at the dose of 15 mg/kg, Q7x2 (every seven days for 2 weeks) and stopped two weeks before the end of the experiment. ISRIB (trans-isomer, Aurogene s.r.l) was dissolved in 10% DMSO (Sigma-Aldrich) in corn oil. It was injected interaperitoneally (i.p.), at the dose 2.5 mg/kg every two days for three weeks. Forty-eight hours after the last dose of ISRIB, mice were sacrificed, and primary breast tumors were isolated and RNA extracted. Protocol: miRNeasy Mini Kit TruSeq Stranded Total RNA Sample Preparation Guide

text: breast cancer

text: ERO1 knockout

characteristic: genotype

text: not applicable

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Istituto di Ricerche Farmacologiche Mario Negri IRCCS

text: public

text: E-MTAB-11313:24_Vehicle_ERO1_KO_S9_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11313:25_Vehicle_ERO1_KO_S10_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: ERO1 knockout

text: not applicable

characteristic: isolate

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11313:1_Vehicle_ERO1_WT_S1_R1_001

text: ArrayExpress

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

characteristic: organism part

text: Homo sapiens


text: public

characteristic: SRA accession

text: E-MTAB-11313:3_Vehicle_ERO1_WT_S2_R1_001

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-11313:4_Vehicle_ERO1_WT_S1_R1_001

text: ArrayExpress

text: human

characteristic: common name

characteristic: description

text: breast cancer

text: wild type genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-11313:5_Vehicle_ERO1_WT_S2_R1_001

text: ArrayExpress

text: human

text: breast cancer

characteristic: disease

text: wild type genotype

characteristic: genotype

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: mammary gland

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:MIMS; Dept. molecular biology Umea university

text: ArrayExpress

text: Protocols: 1ml of Qiazol Lysis Reagent was added to the worm pellets together with 500μl 0.5mm Zirconia beads (BioSpec). Samples were disrupted using Bullet blender (Next Advance) at 4°C. 100μl 1-Bromo-3-chloropropane (Sigma) was added, and the mixture was vortexed and centrifuged for phase separation. 15 day-one adults were picked to a new plate and laid eggs for 2 hours before removed. Eggs were allowed to develop into late L4 stage. 10 plates of L4 animals were collected as one sample, and 5 independent samples were prepared for each genotype. Animals were quickly washed 3 times in M9 buffer, and frozen in liquid nitrogen after M9 was removed. RNA was prepared with RNeasy Plus Universal Mini Kit from Qiagen. Library preparation and RNA sequencing was performed at Novogene.

text: larval stage 4

characteristic: developmental stage

text: il17.1 overexpression

characteristic: individual

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed

characteristic: sex

characteristic: strain


text: MIMS; Dept. molecular biology Umea university

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: larval stage 4

text: il17.1 overexpression

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: mixed

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: description

text: larval stage 4

text: nhr-49 gain of function mutant

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: larval stage 4

characteristic: developmental stage

text: mdt-15 gain of function mutant

characteristic: genotype

characteristic: individual

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: larval stage 4

text: il17.1 overexpression + nhr-49 gain of function mutant

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed

characteristic: sex

characteristic: strain


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: larval stage 4

text: il17.1 overexpression + mdt-15 gain of function mutant

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: mixed

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: larval stage 4

text: il17.1 -/-

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

characteristic: description

text: larval stage 4

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: not applicable

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: larval stage 4

text: nhr-49 -/-

characteristic: individual

text: not applicable

text: Caenorhabditis elegans

characteristic: organism

text: Caenorhabditis elegans

text: whole organism

text: Caenorhabditis elegans

text: mixed

characteristic: sex


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: larval stage 4

text: mdt-15 -/-

text: not applicable

characteristic: isolate

text: Caenorhabditis elegans

text: Caenorhabditis elegans

text: whole organism

characteristic: organism part

text: Caenorhabditis elegans

text: mixed

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM Gustave Roussy institute

text: public

characteristic: SRA accession

tag: Namespace:INSERM Gustave Roussy institute

characteristic: Submitter Id

text: ArrayExpress

text: CD34-negative, CD41-positive, CD42-positive megakaryocyte cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: The CD41+/CD42+ MK were sorted (10 000 to 30 000 cells). Cells were submitted to cell lysis, transposition, and purification steps according to Illumina protocol. The transposed DNA fragments were amplified by PCR 12 times using adapters from the Nextera Index Kit (Illumina). PCR purification was performed using MinElute PCR Purification Kit (Qiagen, 28004) to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies 5067-4626).

text: embryo stage

text: Down syndrome

characteristic: disease

text: trisomic with GATA1s mutation and SMC3 mutation

text: Homo sapiens

text: Homo sapiens

text: skin

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

characteristic: title