characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Streptococcus hyointestinalis

characteristic: project name

characteristic: sample derived from

text: Streptococcus hyointestinalis

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus;s__Streptococcus hyointestinalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812344_bin.27_metaWRAP_v1.3_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812344 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

unit: DD

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Peptostreptococcus russellii

characteristic: organism

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

text: Peptostreptococcus russellii

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Peptostreptococcaceae;g__Peptostreptococcus;s__Peptostreptococcus russellii

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812344_bin.28_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Enterococcus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Enterococcus sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Enterococcaceae;g__Enterococcus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812344_bin.29_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Peptoniphilaceae bacterium

characteristic: organism

text: uncultured Peptoniphilaceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Tissierellia;o__Tissierellales;f__Peptoniphilaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812344_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Faecalitalea sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Faecalitalea sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Faecalitalea;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812344_bin.30_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Mobilibacterium timonense

characteristic: organism

text: Mobilibacterium timonense

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiales Family XIII. Incertae Sedis;g__Mobilibacterium;s__Mobilibacterium timonense

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812344_bin.32_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812355 of study SRP324051.

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.11_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: Lactobacillus johnsonii

characteristic: organism

text: Lactobacillus johnsonii

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus johnsonii

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812355_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

characteristic: project name

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.14_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Roseburia sp.

characteristic: organism

text: uncultured Roseburia sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812355_bin.16_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Lachnobacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnobacterium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnobacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.17_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus reuteri

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812355_bin.2_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.20_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812355 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Erysipelotrichaceae bacterium

characteristic: organism

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812355_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.5_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Treponema sp.

characteristic: organism

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

text: uncultured Treponema sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Spirochaetes;c__Spirochaetia;o__Spirochaetales;f__Spirochaetaceae;g__Treponema;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812355_bin.6_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812355_bin.9_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812366 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812366_bin.13_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Roseburia sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Roseburia sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812366_bin.19_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Coprococcus sp.

characteristic: organism

text: uncultured Coprococcus sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Coprococcus;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812366_bin.21_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812366_bin.6_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812366_bin.9_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812367 of study SRP324051.

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.1_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.10_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.11_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Treponema sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Treponema sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Spirochaetes;c__Spirochaetia;o__Spirochaetales;f__Spirochaetaceae;g__Treponema;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.13_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.15_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Butyrivibrio crossotus

characteristic: project name

characteristic: sample derived from

text: Butyrivibrio crossotus

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Butyrivibrio;s__Butyrivibrio crossotus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.16_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.17_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Escherichia coli

characteristic: project name

characteristic: sample derived from

text: Escherichia coli

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.18_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812367 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.19_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.21_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.24_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.25_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.27_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.28_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: organism

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Ruminococcus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Ruminococcus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.30_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812367 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Lachnobacterium sp.

characteristic: organism

text: uncultured Lachnobacterium sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnobacterium;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.31_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Prevotellaceae bacterium

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.4_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Tenericutes bacterium

characteristic: organism

text: uncultured Tenericutes bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.5_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.6_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812367_bin.7_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Pseudobutyrivibrio sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudobutyrivibrio sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Pseudobutyrivibrio;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812367_bin.9_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812368 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Prevotellaceae bacterium

characteristic: organism

text: uncultured Prevotellaceae bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812368_bin.11_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Mogibacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Mogibacterium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiales Family XIII. Incertae Sedis;g__Mogibacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812368_bin.5_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812369 of study SRP324051.

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Bacteroidales bacterium

characteristic: organism

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.10_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Lactobacillus amylovorus

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

characteristic: project name

characteristic: sample derived from

text: Lactobacillus amylovorus

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus amylovorus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.11_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Parabacteroides sp.

characteristic: organism

text: uncultured Parabacteroides sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Anaerostipes sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Anaerostipes sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Anaerostipes;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.13_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Blautia sp.

characteristic: organism

text: uncultured Blautia sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Blautia;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.15_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: Phascolarctobacterium succinatutens

characteristic: project name

characteristic: sample derived from

text: Phascolarctobacterium succinatutens

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Acidaminococcales;f__Acidaminococcaceae;g__Phascolarctobacterium;s__Phascolarctobacterium succinatutens

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.19_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Clostridium sp.

characteristic: organism

text: uncultured Clostridium sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.2_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR14812369 of study SRP324051.

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.20_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Ruminococcus sp.

characteristic: organism

text: In response to new stringent regulations in Canada on the use of antibiotics in animal production, many farms implemented practices to produce animals that are Raised Without Antibiotics (RWA) from birth to slaughter. This study aimed to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance monitoring of farms that adopted the RWA program and conventional farms using antibiotics as per the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week old pigs and composite ma-nure from the barn over 6 time points and applying whole genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced frequency of AMR genes in piglet feces and in barn manure. However, metagenomic analyses showed that RWA barns had significant increase in the frequency of pathogenic Firmicutes in fecal samples and pathogenic Proteobacteria in barn manure samples.

text: uncultured Ruminococcus sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Ruminococcus;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.21_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.22_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Bacteroides sp.

characteristic: organism

text: uncultured Bacteroides sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.23_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Prevotella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Prevotella sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.24_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Pig digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: collection date

text: CheckM

text: Feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

text: not provided

text: metagenome-assembled genome

text: pig gut metagenome

text: Intestine

text: pig gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR14812369_bin.26_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Pig digestive system

text: EBI-EMG

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Feces

characteristic: environmental medium

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: pig gut metagenome

text: Intestine

characteristic: local environmental context

text: pig gut metagenome

characteristic: metagenomic source

text: uncultured Blautia sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Blautia sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Blautia;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR14812369_bin.28_metaWRAP_v1.3_MAG

characteristic: title