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characteristic: collecting institution

characteristic: collection date

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: GR

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/GR-ETHZ-38003491/2022

text: active surveillance not initiated by an outbreak


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: GISAID Accession ID

characteristic: INSDC last update

text: public

text: SIB

text: Stadtspital Waid, Zürich, Switzerland

text: Switzerland

text: ZH

text: Human

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba661c2/2022

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Switzerland

text: GR

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/GR-ETHZ-38003559/2022

characteristic: organism

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC last update

text: public

text: SIB

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: SZ

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/SZ-ETHZ-38003468/2022


E-MTAB-12139:Sample 1

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12139:Sample 1

tag: Namespace:Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology

unit: month

characteristic: age

text: ArrayExpress

text: bread wheat

characteristic: common name

text: Piko

characteristic: cultivar

text: Protocols: The plant material consisted of two highly-chasmogamous (HCH) cultivars: Piko (DE, 2009, CWW-3319.5/3/Kraka//Maris Huntsman/Fruhgold) and Poezja (LT, 2017, STH43/STH248-93-14-326), and two low-chasmogamy (LCH) cultivars: Euforia (PL, 2018, Muszelka//N5113/STH3907) and KWS Dacanto (PL, 2011, Opus/Certo) cultivars of hexaploid winter wheat. In order to identify candidate genes controlling lodicules opening, lodicules of HCH and LCH genotypes were harvested from spikes at pre-flowering (GS95 - ear completely emerged above flag leaf ligule) corresponding to green anther stage (GAS) and early flowering (GS61 - start of flowering (first anthers visible) referred as yellow anther stage (YAS) (5, 32). The samples were collected in three biological replicates. Approximately 50-100 lodicules were samples from five to seven individual spikes for each of the three replicates. The materials were immediately submerged in RNAlater™ Stabilization Solution (Ambion, Thermo Fisher Scientific, Waltham, MA, USA) and kept at -80˚C until required. After 6-week vernalization at 4˚C, plants were transferred to a growth chamber (ForClean, ZalMed Sp. z.o.o, Warsaw, Poland) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used to isolate total RNA from the preserved lodicules. RNA concentration was measured by means of a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA) and its quality and integrity were verified on BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) using Agilent RNA 6000 Nano Kit. The first and the second strand of cDNA smART were obtained using First Strand cDNA Synthesis Kit (EURx, Gdańsk, Poland) and Second Strand cDNA Synthesis Kit (abm, Richmond, BC, Canada), respectively. Representational difference analysis was performed on high quality cDNA according to Hubank and Schatz (1994) procedure with small modifications. Lodicules at the pre-flowering were used as testers while the lodicules early-flowering were selected as driver probes. The analysis was taken in three replicates for each variety. The tester:driver ratio in three subsequent rounds of subtractive hybridization increased from 1:50, and 1:400 to 1:200000. Differential products obtained in the third subtraction of the size range from 200 to 800 bp were sequenced. Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA) was used for library construction according to the manufacturer instructions.

characteristic: description

text: pre-flowering and early flowering

characteristic: developmental stage

text: Piko

characteristic: genotype

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

characteristic: organism part

characteristic: replicate

text: Triticum aestivum

text: Sample 1


E-MTAB-12139:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12139:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bread wheat

text: Piko

text: pre-flowering and early flowering

text: Piko

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 2

characteristic: title


E-MTAB-12139:Sample 3

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12139:Sample 3

characteristic: age

text: ArrayExpress

text: bread wheat

text: Piko

text: pre-flowering and early flowering

text: Piko

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 3


E-MTAB-12139:Sample 4

text: public

text: E-MTAB-12139:Sample 4

text: ArrayExpress

text: bread wheat

characteristic: common name

text: Poezja

characteristic: cultivar

characteristic: description

text: pre-flowering and early flowering

characteristic: developmental stage

text: Poezja

characteristic: genotype

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 4


E-MTAB-12139:Sample 5

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: E-MTAB-12139:Sample 5

text: ArrayExpress

text: bread wheat

text: Poezja

text: pre-flowering and early flowering

text: Poezja

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

characteristic: organism part

characteristic: replicate

text: Triticum aestivum

text: Sample 5


E-MTAB-12139:Sample 6

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12139:Sample 6

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bread wheat

text: Poezja

text: pre-flowering and early flowering

text: Poezja

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 6


E-MTAB-12139:Sample 7

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12139:Sample 7

text: ArrayExpress

text: bread wheat

text: Euforia

text: pre-flowering and early flowering

text: Euforia

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 7

characteristic: title


E-MTAB-12139:Sample 8

text: public

text: E-MTAB-12139:Sample 8

characteristic: age

text: ArrayExpress

text: bread wheat

characteristic: common name

text: Euforia

characteristic: cultivar

characteristic: description

text: pre-flowering and early flowering

characteristic: developmental stage

text: Euforia

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 8


E-MTAB-12139:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: E-MTAB-12139:Sample 9

text: ArrayExpress

text: bread wheat

text: Euforia

text: pre-flowering and early flowering

text: Euforia

characteristic: genotype

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

characteristic: organism part

text: Triticum aestivum

text: Sample 9


E-MTAB-12139:Sample 10

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12139:Sample 10

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: bread wheat

text: KWS Dacanto

text: pre-flowering and early flowering

text: KWS Dacanto

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

characteristic: replicate

text: Triticum aestivum

text: Sample 10


E-MTAB-12139:Sample 11

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12139:Sample 11

text: ArrayExpress

text: bread wheat

text: KWS Dacanto

text: pre-flowering and early flowering

text: KWS Dacanto

text: Triticum aestivum

characteristic: organism

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 11


E-MTAB-12139:Sample 12

text: public

text: E-MTAB-12139:Sample 12

characteristic: age

text: ArrayExpress

text: bread wheat

characteristic: common name

text: KWS Dacanto

characteristic: cultivar

characteristic: description

text: pre-flowering and early flowering

text: KWS Dacanto

text: Triticum aestivum

text: Triticum aestivum

text: lodicule

text: Triticum aestivum

text: Sample 12

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ETH ZURICH

characteristic: INSDC last update

text: public

tag: Namespace:ETH ZURICH

text: blood

text: cattle

text: Bos taurus

text: Bos taurus

text: Brown Swiss


text: ETH ZURICH

text: public

characteristic: INSDC status

characteristic: SRA accession

text: blood

text: cattle

text: Bos taurus

text: Bos taurus

text: Original Braunvieh


characteristic: GISAID Accession ID

text: public

tag: Namespace:Laboratory of genomics and metagenomics, Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Trestan Pillonel, Damien Jacot, Sébastien Aeby, Gilbert Greub, Claire Bertelli

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN5350/2020

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Anthony Smith, NICD

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Anthony Smith, NICD

text: EnteroBase

text: EnteroBase

text: A. Smith, National Institute for Communicable Diseases

text: Whole genome sequencing data uploaded by EnteroBase user

characteristic: description

text: South Africa, North West

characteristic: geographic location

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Typhi

characteristic: serovar

characteristic: strain

characteristic: title


text: The Sainsbury Laboratory

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: East Anglia | Norfolk | Winterton on Sea | Winterton Dunes

characteristic: geographic location (region and locality)

text: on ground of coastal dunes just above the beach

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: WHOLE ORGANISM

characteristic: organism part

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

text: selected by hand while hiking dunes stored in paper packet

characteristic: sample collection device or method

text: Cladonia portentosa

text: 47e786f8-32b6-440b-9620-a19627f79a81-ASG-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

characteristic: organism

text: WHOLE ORGANISM

text: ASG

text: NOT PROVIDED

text: Cladonia diversa

text: 0168cdf4-a3fc-4de3-8436-cc66f48adbcc-ASG-specimen

characteristic: title


text: The Sainsbury Laboratory

text: NOT PROVIDED

text: EarlhamInstitute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: WHOLE ORGANISM

text: ASG

text: NOT PROVIDED

text: Xanthoria parietina

text: 1fa619d5-032a-45a8-96db-8e7c67116826-ASG-specimen


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

characteristic: sample derived from

text: Cladonia portentosa

text: NOT PROVIDED

text: N

characteristic: symbiont

text: ce72688e-b04e-4231-a5c2-3c5a76ccdb3f-asg

characteristic: tolid


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

characteristic: organism

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: b86568a1-6297-41ae-aeb0-47e6273ee1ed-asg

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: 4301ee0a-a241-4d1e-b673-124b0a193ba6-asg


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Cladonia portentosa

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 9ed21edf-e8d3-4c51-aaec-f8f2f141d538-asg

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: 13fd9fc9-796b-46dd-852f-4d1bbac1de55-asg

characteristic: title


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: 01b48e84-fe14-443d-8744-1a94738b51e2-asg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Cladonia portentosa

text: NOT PROVIDED

text: N

characteristic: symbiont

text: aaeb624a-54ff-47f7-abc6-b7430f3df899-asg

characteristic: tolid


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: e49457b9-398e-489d-a408-58cc4a9fe468-asg

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Cladonia portentosa

text: NOT PROVIDED

text: N

text: 4ff11005-ad2f-4114-810c-17aad6488d29-asg


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia portentosa

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Cladonia portentosa

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 6a7b203e-7503-4fd7-8368-388ee7977ef0-asg

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Cladonia diversa

text: NOT PROVIDED

text: N

text: 64207b5d-6484-436f-a575-ff11d04bf512-asg

characteristic: title


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: East Anglia | Norfolk | Winterton on Sea | Winterton Dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Cladonia diversa

text: NOT PROVIDED

text: N

text: 8499aa0f-aaf8-43ac-a0b6-e62c883f4bc3-asg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on ground of coastal dunes just above the beach

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

text: selected by hand while hiking dunes stored in paper packet

characteristic: sample derived from

text: Cladonia diversa

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 7ac48f47-e1b2-4f23-9fc8-4f391b870af7-asg

characteristic: tolid


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Cladonia diversa

text: NOT PROVIDED

text: N

text: 0ff0e2af-b32c-4736-bb96-a93845bfaa12-asg

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Cladonia diversa

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Cladonia diversa

text: NOT PROVIDED

text: N

text: badd9eaf-b5e5-4571-b821-a8053eb391ef-asg


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: on branches of oak in coastal dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Xanthoria parietina

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 92e71154-94ff-4862-bd8f-8d092dc5022d-asg

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: 201f960a-d1e3-4335-a095-e2fb3cd75890-asg

characteristic: title


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: on branches of oak in coastal dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: 8f7c4fa1-b58d-4b15-be03-0a0ae640d58e-asg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Xanthoria parietina

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 7af6fdc4-f931-42c3-84ef-1962b27df51a-asg

characteristic: tolid


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: on branches of oak in coastal dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: 4e6120c3-8cca-400f-b330-88d925b4fc02-asg

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: 484e2760-15a6-4407-af5c-da137a59a1d9-asg


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: on branches of oak in coastal dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Xanthoria parietina

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 81160ee0-acd9-42a3-aa64-25e96b7a26d8-asg

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

characteristic: organism

text: **OTHER FUNGAL TISSUE**

characteristic: organism part

text: ASG

text: NOT PROVIDED

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: fd08bd38-ddba-401f-a75b-05c10914db7a-asg

characteristic: title


text: The Sainsbury Laboratory

text: EarlhamInstitute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: THE SAINSBURY LABORATORY

characteristic: barcoding center

text: COPO

characteristic: broker name

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

characteristic: collecting institution

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: on branches of oak in coastal dunes

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

characteristic: project name

text: NOT PROVIDED

characteristic: proxy voucher

characteristic: sample collection device or method

text: Xanthoria parietina

text: NOT PROVIDED

text: N

text: a32b0324-aed0-4665-b511-c45c97d1d8dc-asg


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Sainsbury Laboratory

characteristic: GAL

text: EarlhamInstitute

characteristic: INSDC center name

text: public

text: THE SAINSBURY LABORATORY

text: COPO

text: KLARA SCHARNAGL | NICK TALBOT

text: UC BERKELEY | THE SAINSBURY LABORATORY

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: on branches of oak in coastal dunes

characteristic: habitat

text: KLARA SCHARNAGL

text: UC BERKELEY

text: Xanthoria parietina

text: **OTHER FUNGAL TISSUE**

text: ASG

text: NOT PROVIDED

characteristic: sample derived from

text: Xanthoria parietina

text: NOT PROVIDED

text: N

characteristic: symbiont

text: 94b86a22-064f-4d39-976f-18ee8e01e45c-asg

characteristic: tolid


text: EMG

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3.0

characteristic: binning software

text: plant

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR17254981 of study SRP351203.

characteristic: description

text: rhizosphere

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: rhizosphere metagenome

text: tomato

characteristic: local environmental context

text: rhizosphere metagenome

characteristic: metagenomic source

text: uncultured Salinimicrobium sp.

characteristic: organism

text: Here, taxonomic and (meta)genomic features of the rhizosphere microbiome were mapped as quantitative traits of a tomato recombinant inbred line population. This is the metagenomic part of the project (the first part (amplicon sequences) is stored under the BioProject PRJNA787039).

text: uncultured Salinimicrobium sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Flavobacteriia;o__Flavobacteriales;f__Flavobacteriaceae;g__Salinimicrobium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR17254981_bin.2_metawrap_v1.3.0_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3.0

characteristic: binning software

text: plant

characteristic: broad-scale environmental context

text: EBI-EMG

text: CheckM

text: rhizosphere

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: rhizosphere metagenome

text: tomato

text: rhizosphere metagenome

text: uncultured Marinobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Marinobacter sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Alteromonadales;f__Alteromonadaceae;g__Marinobacter;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR17254981_bin.3_metawrap_v1.3.0_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3.0

text: plant

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: rhizosphere

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: rhizosphere metagenome

text: tomato

characteristic: local environmental context

text: rhizosphere metagenome

characteristic: metagenomic source

text: uncultured Actinotalea sp.

characteristic: organism

text: uncultured Actinotalea sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Micrococcales;f__Cellulomonadaceae;g__Actinotalea;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR17254981_bin.4_metawrap_v1.3.0_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3.0

characteristic: binning software

text: plant

characteristic: broad-scale environmental context

text: EBI-EMG

text: CheckM

text: rhizosphere

text: Netherlands

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: rhizosphere metagenome

text: tomato

text: rhizosphere metagenome

text: uncultured Altererythrobacter sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Altererythrobacter sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Sphingomonadales;f__Erythrobacteraceae;g__Altererythrobacter;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR17254981_bin.6_metawrap_v1.3.0_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3.0

text: plant

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: rhizosphere

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: rhizosphere metagenome

text: tomato

characteristic: local environmental context

text: rhizosphere metagenome

characteristic: metagenomic source

text: uncultured Altererythrobacter sp.

characteristic: organism

text: uncultured Altererythrobacter sp.

text: Illumina HiSeq 4000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR17254981_bin.7_metawrap_v1.3.0_MAG

characteristic: title