E-MTAB-12124:Sample 41

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 41

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 41


E-MTAB-12124:Sample 42

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 42

text: 40 to 85

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: Protocols: Libraries were prepared from samples of 74 patients using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus, with additional custom depletion probes, using 100ng RNA. Libraries were sequenced on the Illumina NovaSeqTM 6000 using 100 base paired-end chemistry (108 - 455 million read-pairs per sample). Sequencing reads were processed and aligned to Human Reference genome GRCh38 using the Illumina Dragen RNA pipeline v3.8.4. Libraries were prepared from samples of 74 patients using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus, with additional custom depletion probes, using 100ng RNA. Libraries were sequenced on the Illumina NovaSeqTM 6000 using 100 base paired-end chemistry (108 - 455 million read-pairs per sample). Sequencing reads were processed and aligned to Human Reference genome GRCh38 using the Illumina Dragen RNA pipeline v3.8.4.

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 42

characteristic: title


E-MTAB-12124:Sample 44

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 44

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 44


E-MTAB-12124:Sample 45

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 45

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 45


E-MTAB-12124:Sample 43

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 43

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 43


E-MTAB-12124:Sample 46

text: OXFORD

text: public

text: E-MTAB-12124:Sample 46

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 46


E-MTAB-12124:Sample 47

text: OXFORD

text: public

text: E-MTAB-12124:Sample 47

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 47

characteristic: title


E-MTAB-12124:Sample 49

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 49

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 49


E-MTAB-12124:Sample 48

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 48

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 48


E-MTAB-12124:Sample 51

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 51

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 51


E-MTAB-12124:Sample 56

text: OXFORD

text: public

text: E-MTAB-12124:Sample 56

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 56


E-MTAB-12124:Sample 58

text: OXFORD

text: public

text: E-MTAB-12124:Sample 58

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 58

characteristic: title


E-MTAB-12124:Sample 50

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 50

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 50


E-MTAB-12124:Sample 52

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 52

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 52


E-MTAB-12124:Sample 59

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 59

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 59


E-MTAB-12124:Sample 57

text: OXFORD

text: public

text: E-MTAB-12124:Sample 57

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 57


E-MTAB-12124:Sample 53

text: OXFORD

text: public

text: E-MTAB-12124:Sample 53

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 53

characteristic: title


E-MTAB-12124:Sample 55

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 55

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 55


E-MTAB-12124:Sample 65

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 65

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 65


E-MTAB-12124:Sample 62

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 62

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 62


E-MTAB-12124:Sample 61

text: OXFORD

text: public

text: E-MTAB-12124:Sample 61

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 61


E-MTAB-12124:Sample 64

text: OXFORD

text: public

text: E-MTAB-12124:Sample 64

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 64

characteristic: title


E-MTAB-12124:Sample 63

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 63

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 63


E-MTAB-12124:Sample 60

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 60

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 60


E-MTAB-12124:Sample 68

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 68

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 68


E-MTAB-12124:Sample 73

text: OXFORD

text: public

text: E-MTAB-12124:Sample 73

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 73


E-MTAB-12124:Sample 70

text: OXFORD

text: public

text: E-MTAB-12124:Sample 70

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 70

characteristic: title


E-MTAB-12124:Sample 66

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 66

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 66


E-MTAB-12124:Sample 72

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

text: public

text: E-MTAB-12124:Sample 72

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 72


E-MTAB-12124:Sample 69

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 69

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 69


E-MTAB-12124:Sample 74

text: OXFORD

text: public

text: E-MTAB-12124:Sample 74

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 74


E-MTAB-12124:Sample 71

text: OXFORD

text: public

text: E-MTAB-12124:Sample 71

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 71

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Greifswald, Institute of Pharmacy

characteristic: Submitter Id

text: WGS sample

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: isolate

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: sequence type

characteristic: strain

text: Klebsiella pneumoniae PBIO3459 DNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Greifswald, Institute of Pharmacy

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: colony from LB agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 LB RNA Replicate A


text: public

text: colony from LB agar

characteristic: description

text: Germany

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 LB RNA Replicate B


text: public

text: colony from LB agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 LB RNA Replicate C

characteristic: title


characteristic: INSDC last update

text: public

text: colony from chromogenic agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 C RNA Replicate A


text: public

characteristic: INSDC status

text: colony from chromogenic agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 C RNA Replicate B


text: public

characteristic: SRA accession

text: colony from chromogenic agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 C RNA Replicate C


text: public

characteristic: Submitter Id

text: colony from BHI agar

text: Germany

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 BHI RNA Replicate A


text: public

text: colony from BHI agar

text: Germany

characteristic: geographic location (country and/or sea)

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 BHI RNA Replicate B


text: public

text: colony from BHI agar

text: Germany

characteristic: isolate

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae PBIO3459 BHI RNA Replicate C


unit: m

characteristic: Depth

text: 2019-06-21T11:40:00Z/2019-06-21T11:42:00Z

characteristic: Event Date/Time

text: Ocean Sampling Day Consortium

text: public

unit: DD

characteristic: Latitude Start

characteristic: Longitude Start

characteristic: Marine Region

characteristic: Protocol Label

unit: practical salinity unit

characteristic: Salinity

characteristic: Sampling Campaign

text: Jon Boat

characteristic: Sampling Platform

text: OSD60, South Carolina North Inlet USA

characteristic: Sampling Site

text: 68693:39ccef70-9d22-418e-be91-3cdc57660f83

tag: Namespace:Ocean Sampling Day Consortium

unit: ºC

characteristic: Temperature

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

text: surface water sample from the North Inlet in N. Atlantic

text: sea water [ENVO:00002149]

characteristic: environmental medium

text: water

characteristic: environmental package

text: Prokaryotic samples

text: 2L Nalgene bottles

text: Hand dipped, submerged under water to fill bottle

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: marine metagenome

text: Ocean Sampling Day

characteristic: project name

text: Sterivex

text: particulate matter

characteristic: sample_filtration time (min)

characteristic: sample_quantity (l)

text: no prefiltration

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

text: water

characteristic: water environmental package


characteristic: Depth

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: 2019-06-21T10:39:00Z/2019-06-21T10:45:00Z

characteristic: Event Date/Time

characteristic: External Id

text: Ocean Sampling Day Consortium

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude Start

characteristic: Longitude Start

characteristic: Marine Region

characteristic: Protocol Label

characteristic: SRA accession

text: off cove by South Carolina Department at Natural Resources

text: OSD39, Charleston Harbor USA

text: 68686:39ccef70-9d22-418e-be91-3cdc57660f83

characteristic: Submitter Id

text: marine biome [ENVO:00000447]

text: GFBIO

text: surface water sample from the Charleston Harbor

characteristic: description

text: sea water [ENVO:00002149]

text: water

text: Prokaryotic samples

text: 2L Nalgene bottle

text: Submerged under surface to fill the bottle by hand

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: Ocean Sampling Day

text: Sterivex

text: particulate matter

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Ocean Sampling Day Consortium

text: public

text: 68677:39ccef70-9d22-418e-be91-3cdc57660f83

text: GFBIO

characteristic: broker name

text: PCR blank

text: marine metagenome

text: PCR blank

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Ocean Sampling Day Consortium

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 68676:39ccef70-9d22-418e-be91-3cdc57660f83

text: GFBIO

text: Extraction blank

text: marine metagenome

text: Extraction blank

text: marine metagenome

text: Illumina MiSeq

text: true


text: IHU - Mediterranee Infection

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:IHU - Mediterranee Infection

text: A new strain of Minimicrobia massiliensis genome isolated from saliva.

text: saliva

text: Candidatus Saccharibacteria bacterium

text: Candidatus Saccharibacteria bacterium

text: Minimicrobia massiliensis genome


text: public

tag: Namespace:CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER

text: 8 to 9

unit: week

characteristic: age

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: mice were sacrificed by exsanguination, lungs collected and placed on ice, minced, digested in collagenase, elastase, dispase, DNAse, and Lich enzyme mix at 4oC, RBCs lysed, and passed through 30 micron filter, and single cells suspended and labeled with 10X Cellplex lipid-conjugated oligos. Dead cells were excluded using DAPI and flow cytometry with 50 micron nozzle which selected for smaller, immune cells. cDNA libraries were created from GEM using a 10X Chromium controller after loading 16,000 cells per well into 4 wells. cDNA libraries were created using the v3.3 protocol, the Dual Index TT and NN kits, and 16 cycles. Feature barcode libraries were created using a 10X accessory kit

text: adult

characteristic: developmental stage

text: Cela1 -/-

characteristic: genotype

text: KO4780, KO4783, KO4782

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: KOPBS


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: 9 to 11

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: description

text: adult

text: Cela1 -/-

text: KO4760, KO4762, KO4759

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lung

text: Mus musculus

text: male

text: tracheal porcine pancreatic elastase

characteristic: stimulus

text: KOPPE

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: 10 to 11

characteristic: age

text: ArrayExpress

text: house mouse

text: adult

text: wild type genotype

text: WT4805, WT4754, WT4758

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: male

text: WTPBS


text: public

text: 10 to 11

text: ArrayExpress

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: WT4757, WT4753, WT4756

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: tracheal porcine pancreatic elastase

characteristic: strain

text: WTPPE


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: GISAID Accession ID

text: Dr. Risch

text: public

characteristic: SRA accession

tag: Namespace:Dr. Risch

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Dr. Risch Ostschweiz AG, Lagerstrasse 30, 9470 Buchs

characteristic: collecting institution

characteristic: collection date

text: Nadia Wohlwend, Faina Wehrli, Dominique Fabien Hilti, Sinem Kas, Martin Risch, Thomas Bodmer, Lorenz Risch

characteristic: collector name

text: Liechtenstein

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Liechtenstein/FL-Risch-20220831R13328/2022

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: active surveillance not initiated by an outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: description

text: Arthrobacter sp. Marseille-P9274

text: Arthrobacter sp. Marseille-P9274

characteristic: strain

text: Arthrobacter massiliensis genome


text: IHU - Mediterranee Infection

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Aeromonas sp. Marseille-Q5825

characteristic: organism

text: Aeromonas sp. Marseille-Q5825

text: Aeromonas massiliensis genome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IHU - Mediterranee Infection

characteristic: INSDC last update

text: public

text: Anaerococcus sp. Marseille-Q5996

text: Anaerococcus sp. Marseille-Q5996

text: Anaerococcus pneumoniae genome


text: IHU - Mediterranee Infection

text: public

text: Chryseobacterium sp. Marseille-Q5410

text: Chryseobacterium sp. Marseille-Q5410

text: Chryseobacterium marseillensis genome


text: NIPH

text: public

text: ELIXIR-Norway

characteristic: broker name

text: N. meningitidis

characteristic: common name

text: Norway

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Blood

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NIPH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ELIXIR-Norway

text: N. meningitidis

text: Norway

text: not provided

text: Homo sapiens

text: nasopharyngeal secretion

text: Neisseria meningitidis

text: Neisseria meningitidis


text: IHU - Mediterranee Infection

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: description

text: Clostridium sp. Marseille-Q5894

text: Clostridium sp. Marseille-Q5894

characteristic: strain

text: Clostridium caudasuis genome


characteristic: ENA-CHECKLIST

text: IHU - Mediterranee Infection

text: public

characteristic: Submitter Id

text: Listeria sp. Marseille-Q6474

characteristic: organism

text: Listeria sp. Marseille-Q6474

text: Listeria massiliensis genome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: IHU - Mediterranee Infection

characteristic: INSDC last update

text: public

text: Neisseria sp. Marseille-Q5346

text: Neisseria sp. Marseille-Q5346

text: Neisseria ossis genome


text: IHU - Mediterranee Infection

text: public

text: Pantoea sp. Marseille-Q5743

text: Pantoea sp. Marseille-Q5743

text: Pantoea timonensis genome


text: IHU - Mediterranee Infection

text: public

text: Pseudomonas sp. Marseille-Q5117

text: Pseudomonas sp. Marseille-Q5117

text: Pseudomonas sputimassiliensis genome


text: IHU - Mediterranee Infection

text: public

text: Saccharococcus sp. Marseille-Q5394

text: Saccharococcus sp. Marseille-Q5394

text: Saccharococcus massiliensis genome


text: IHU - Mediterranee Infection

text: public

text: Stenotrophomonas sp. Marseille-Q5258

text: Stenotrophomonas sp. Marseille-Q5258

text: Stenotrophomonas timonensis genome


text: IHU - Mediterranee Infection

text: public

text: Streptococcus sp. Marseille-Q5619

text: Streptococcus sp. Marseille-Q5619

text: Streptococcus paraosteomassiliensis genome


text: IHU - Mediterranee Infection

text: public

text: Streptococcus sp. Marseille-Q5855

text: Streptococcus sp. Marseille-Q5855

text: Streptococcus parapneumoniae genome


text: IHU - Mediterranee Infection

text: public

text: Streptococcus sp. Marseille-Q5986

text: Streptococcus sp. Marseille-Q5986

text: Streptococcus urinitimonensis genome


text: IHU - Mediterranee Infection

text: public

text: Streptococcus sp. Marseille-Q6488

text: Streptococcus sp. Marseille-Q6488

text: Streptococcus vaginomassiliensis genome


text: IHU - Mediterranee Infection

text: public

text: Streptococcus sp. Marseille-Q6470

text: Streptococcus sp. Marseille-Q6470

text: Streptococcus vasi genome


text: IHU - Mediterranee Infection

text: public

text: Yersinia sp. Marseille-Q5920

text: Yersinia sp. Marseille-Q5920

text: Yersinia timonensis genome


text: public

tag: Namespace:Institut Necker-Enfants Malades, INSERM U1151/CNRS UMR 8253, Universite Paris Descartes, Sorbonne Paris Cite, 75993 Paris Cedex 14, France.

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

characteristic: common name

text: Protocols: B220+EYFP+PNA+GL7+ GC B cells, B220+EYFP+PNA-GL7- memory B cells and B220+EYFP-IgD+ and from dLNs of aP:wP, wP:aP and wP:wP vaccinated mice were first enriched by cell sorting, then viable GC and memory B cells were single cell FACS-sorted (AriaIII) into 384-well capture plates (Single Cell Discoveries) containing 384 primers and Mineral oil (Sigma) and immediately stored at -80°C. aP:aP dLN cells were first FACS enriched as B220+EYFP+ cells, due to their lower cell frequency, then B220+EYFP+PNA+GL7+ GC and B220+EYFP+PNA-GL7- memory B cells were single-cell sorted. The “Well id”, \Cell Type\, “Stimulus” attributes in plate layouts recapitulate all sorting information for each cell. This dataset was performed in three distinct batches: batch 1 [Samples: V01 to V05], batch 2 [Samples: V06 to V09], and batch 3 [Samples V10 to V12]. Each sample corresponds to a sorted 384 plate containing cells from one or multiple mice. A layout for each sample was added to recapitulate all the information needed for the correct analysis, such as “cell.type”, “stimulus”, “day.sorted”, “mouse.id” but also information referring to the status of markers “INDEX_” used for the sort : GL7, PNA and surface markers for downstream analysis : Immunoglobulin status for each sorted cell. All plates from each of the three batches, regardless of the sorting date, were processed at the same time for library preparation and sequencing. Mice were subcutaneously (s.c) injected at the base of the tail with 100 µl of aP and wP vaccine formulations, provided by Sanofi-Pasteur, Marcy l’Etoile (Lyon), which correspond to 1/5 of the human dose but do not correspond to commercial vaccines as they lack additional components like tetanus or diphtheria toxins. The aP vaccine is composed of 20 μg/ml of pertussis toxoid (PT), 6 μg/ml of pertactin (PRN), 10 μg/ml of fimbriae 2,3 (Fim2,3), 10 μg/ml of filamentous haemagglutinin (FHA) and 0.66 mg/ml alum; the wP vaccine is made of 30 OU/ml whole cell pertussis and 2.4 mg/ml alum.Doses of 10 mg/mouse Nolvadex (Tamoxifen; AstraZeneca) in 300 μl of 20% Clinoleic (Baxter) were administered by gavage at day 7 , 10 and 31 post boost injection. Cells were heat-lysed at 65° C followed by cDNA synthesis After second-strand cDNA synthesis, all the barcoded material from one plate was pooled into one library and amplified using in vitro transcription (IVT). Following amplification, library preparation was done following the CEL-Seq2 protocol (Hashimshony T, Senderovich N, Avital G, Klochendler A, de Leeuw Y, Anavy L, Gennert D, Li S, Livak KJ, Rozenblatt-Rosen O, Dor Y, Regev A, Yanai I. CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq. Genome Biol. 2016 Apr 28;17:77. doi: 10.1186/s13059-016-0938-8.) to prepare a cDNA library for sequencing using TruSeq small RNA primers (Illumina)

text: adult

characteristic: developmental stage

text: AID-Cre-EYFP

characteristic: genotype

text: See Layout V01

characteristic: immunophenotype

text: See Layout V01

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: Mus musculus

text: See Layout V01

characteristic: sex

text: See Layout V01

characteristic: stimulus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut Necker-Enfants Malades, INSERM U1151/CNRS UMR 8253, Universite Paris Descartes, Sorbonne Paris Cite, 75993 Paris Cedex 14, France.

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: B cell

text: house mouse

characteristic: description

text: adult

text: AID-Cre-EYFP

text: See Layout V02

text: See Layout V02

text: Mus musculus

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V02

text: See Layout V02

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

text: adult

text: AID-Cre-EYFP

text: See Layout V03

text: See Layout V03

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V03

text: See Layout V03

characteristic: title


text: public

text: ArrayExpress

text: B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: AID-Cre-EYFP

characteristic: genotype

text: See Layout V04

characteristic: immunophenotype

text: See Layout V04

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: Mus musculus

text: See Layout V04

characteristic: sex

text: See Layout V04

characteristic: stimulus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: B cell

text: house mouse

characteristic: description

text: adult

text: AID-Cre-EYFP

text: See Layout V05

text: See Layout V05

text: Mus musculus

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V05

text: See Layout V05

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

text: adult

text: AID-Cre-EYFP

text: See Layout V06

text: See Layout V06

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V06

text: See Layout V06

characteristic: title


text: public

text: ArrayExpress

text: B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: AID-Cre-EYFP

characteristic: genotype

text: See Layout V07

characteristic: immunophenotype

text: See Layout V07

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: Mus musculus

text: See Layout V07

characteristic: sex

text: See Layout V07

characteristic: stimulus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: B cell

text: house mouse

characteristic: description

text: adult

text: AID-Cre-EYFP

text: See Layout V08

text: See Layout V08

text: Mus musculus

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V08

text: See Layout V08

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

text: adult

text: AID-Cre-EYFP

text: See Layout V09

text: See Layout V09

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V09

text: See Layout V09

characteristic: title


text: public

text: ArrayExpress

text: B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: AID-Cre-EYFP

characteristic: genotype

text: See Layout V10

characteristic: immunophenotype

text: See Layout V10

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lymph node

characteristic: organism part

text: Mus musculus

text: See Layout V10

characteristic: sex

text: See Layout V10

characteristic: stimulus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: B cell

text: house mouse

characteristic: description

text: adult

text: AID-Cre-EYFP

text: See Layout V11

text: See Layout V11

text: Mus musculus

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V11

text: See Layout V11

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

text: adult

text: AID-Cre-EYFP

text: See Layout V12

text: See Layout V12

text: Mus musculus

characteristic: organism

text: Mus musculus

text: lymph node

text: Mus musculus

text: See Layout V12

text: See Layout V12

characteristic: title


text: SC

text: public

text: SC

text: Rosalind Franklin Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Samples prepared at Rosalind Franklin Laboratory by Donald Fraser, Suki Lee, Rob Howes, The Rosalind Franklin Laboratory and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Wales

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

text: Rosalind Franklin Laboratory

text: not provided

characteristic: description

text: United Kingdom

text: England

text: Human

text: not provided

text: Human

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak


text: SC

text: public

text: SC

characteristic: broker name

text: Rosalind Franklin Laboratory

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Scotland

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: SC

text: Lighthouse Lab in Glasgow

text: not provided

text: Samples prepared at Lighthouse Lab in Glasgow by Harper VanSteenhouse, Yumi Kasai, David Gray, Carol Clugston, Anna Dominiczak and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

text: England

text: Human

text: not provided

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status


text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Lab in Glasgow

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: description

text: United Kingdom

text: Scotland

text: Human

text: not provided

text: Human

text: not provided

text: not provided

text: not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: SC

text: Lighthouse Laboratory Plymouth

text: not provided

text: Samples prepared at Lighthouse Laboratory Plymouth by Lighthouse Laboratory Plymouth and Alex Alderton, Roberto Amato, Jeffrey Barrett, Sonia Goncalves, Ewan Harrison, David K. Jackson, Ian Johnston, Dominic Kwiatkowski, Cordelia Langford, John Sillitoe on behalf of the Wellcome Sanger Institute COVID-19 Surveillance Team (https://www.sanger.ac.uk/covid-team)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: England

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

text: not provided

text: not provided

text: not provided

characteristic: organism

characteristic: title


text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: SC

text: Lighthouse Laboratory Plymouth

text: not provided

text: United Kingdom

text: Scotland

text: Human

text: not provided

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

characteristic: Submitter Id

text: SC

characteristic: broker name

text: Lighthouse Laboratory Plymouth

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: description

text: United Kingdom

text: Wales

text: Human

text: not provided

text: Human

text: not provided

text: not provided

text: not provided


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:EUROPEAN RESEARCH INSTITUTE FOR THE BIOLOGY OF AGEING, UNIVERSITY OF GRONINGEN, UNIVERSITY MEDICAL CENTER GRONINGEN

text: human

characteristic: common name

text: RPE1 cells treated with DMSO for 24hrs

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: EUROPEAN RESEARCH INSTITUTE FOR THE BIOLOGY OF AGEING, UNIVERSITY OF GRONINGEN, UNIVERSITY MEDICAL CENTER GRONINGEN

text: public

characteristic: SRA accession

text: human

text: BJ cells treated with DMSO for 24hrs

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: human

text: BJ cells treated with DMSO for 24hrs then sorted using flow cytometry to identify late G1-early S phase fraction

text: Homo sapiens

text: Homo sapiens


characteristic: External Id

text: public

characteristic: Submitter Id

text: human

text: BJ cells treated with DMSO for 24hrs then sorted using flow cytometry to identify early-mid S phase fraction

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: RPE1 cells treated with DMSO for 24hrs then sorted using flow cytometry to identify late G1-early S phase fraction

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: RPE1 cells treated with DMSO for 24hrs then sorted using flow cytometry to identify early-mid S phase fraction

text: Homo sapiens

text: Homo sapiens


text: public

text: human

text: RPE1 cells treated with DMSO for 24hrs then sorted using flow cytometry to identify mid-late S phase fraction

text: Homo sapiens

text: Homo sapiens