characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR12830929 of study SRP287478.

text: Vagina

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Prevotella timonensis

text: Prevotella timonensis

text: Illumina HiSeq 1500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella timonensis

text: multi-marker approach

text: Metagenome-assembled genome: SRR12830929_bin.10_metaWRAP_v1.3_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

text: not provided

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

unit: DD

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Gardnerella vaginalis

characteristic: organism

text: WGS was conducted on DNA obtained from 20 vaginal swabs collected at four-time points over a period of 12 months from five randomly selected African-American (AA) women. The baseline visit (first-time point) included all women who were diagnosed with asymptomatic BV and were untreated. All subjects were tested subsequently once every two months and received a course of metronidazole for each episode of BV during the 12 months period.

characteristic: project name

characteristic: sample derived from

text: Gardnerella vaginalis

text: Illumina HiSeq 1500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Gardnerella;s__Gardnerella vaginalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR12830929_bin.11_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

text: CheckM

text: Vagina

text: not provided

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Veillonellaceae bacterium

text: uncultured Veillonellaceae bacterium

text: Illumina HiSeq 1500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR12830929_bin.4_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9530650 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Mageeibacillus indolicus

characteristic: organism

text: The vaginal microbiota plays an important role in the health of the female reproductive tract and is closely associated with a variety of pregnancy outcomes and sexually transmitted diseases. A variety of internal and external factors (such as geography, ethnicity, and diet) have a strong influence on the changes in a woman's vaginal microbiome. The effect of a high-altitude living environment on female vaginal microbiota has not been described. In this study, we characterized the vaginal bacteriome and virome of 13 and 34 healthy women living in high-altitude and sea-level areas, respectively, using whole-metagenome shotgun sequencing of their vaginal mucus samples.

characteristic: project name

characteristic: sample derived from

text: Mageeibacillus indolicus

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Hungateiclostridiaceae;g__Mageeibacillus;s__Mageeibacillus indolicus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530650_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Mobiluncus mulieris

text: Mobiluncus mulieris

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Actinomycetaceae;g__Mobiluncus;s__Mobiluncus mulieris

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530650_bin.10_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Arcanobacterium sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Arcanobacterium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Actinomycetaceae;g__Arcanobacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530650_bin.14_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Prevotella sp.

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530650_bin.3_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Porphyromonas gingivalis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Porphyromonas gingivalis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__Porphyromonas;s__Porphyromonas gingivalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530650_bin.7_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Prevotella sp.

text: uncultured Prevotella sp.

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530650_bin.8_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9530663 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Prevotella buccalis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Prevotella buccalis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella buccalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530663_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Lachnospiraceae bacterium

text: The vaginal microbiota plays an important role in the health of the female reproductive tract and is closely associated with a variety of pregnancy outcomes and sexually transmitted diseases. A variety of internal and external factors (such as geography, ethnicity, and diet) have a strong influence on the changes in a woman's vaginal microbiome. The effect of a high-altitude living environment on female vaginal microbiota has not been described. In this study, we characterized the vaginal bacteriome and virome of 13 and 34 healthy women living in high-altitude and sea-level areas, respectively, using whole-metagenome shotgun sequencing of their vaginal mucus samples.

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530663_bin.13_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530663_bin.14_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Prevotella amnii

text: Prevotella amnii

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella amnii

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530663_bin.15_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Megasphaera sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Megasphaera sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Megasphaera;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530663_bin.2_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Atopobium sp.

text: uncultured Atopobium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Atopobium;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530663_bin.5_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9530663 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Gardnerella sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Gardnerella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Gardnerella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9530663_bin.6_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Sneathia sp.

text: The vaginal microbiota plays an important role in the health of the female reproductive tract and is closely associated with a variety of pregnancy outcomes and sexually transmitted diseases. A variety of internal and external factors (such as geography, ethnicity, and diet) have a strong influence on the changes in a woman's vaginal microbiome. The effect of a high-altitude living environment on female vaginal microbiota has not been described. In this study, we characterized the vaginal bacteriome and virome of 13 and 34 healthy women living in high-altitude and sea-level areas, respectively, using whole-metagenome shotgun sequencing of their vaginal mucus samples.

text: uncultured Sneathia sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Fusobacteria;c__Fusobacteriia;o__Fusobacteriales;f__Leptotrichiaceae;g__Sneathia;s__Sneathia amnii

text: multi-marker approach

text: Metagenome-assembled genome: ERR9530663_bin.9_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9535635 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Atopobium sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Atopobium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Atopobium;s__Atopobium vaginae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9535635_bin.11_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Gardnerella vaginalis

text: Gardnerella vaginalis

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Gardnerella;s__Gardnerella vaginalis

text: multi-marker approach

text: Metagenome-assembled genome: ERR9535635_bin.9_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538571 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Gardnerella vaginalis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Gardnerella vaginalis

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538571_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538591 of study ERP136557.

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Gardnerella vaginalis

text: Gardnerella vaginalis

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538591_bin.1_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538619 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Gardnerella vaginalis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Gardnerella vaginalis

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538619_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538693 of study ERP136557.

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Lactobacillus crispatus

text: Lactobacillus crispatus

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus crispatus

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538693_bin.1_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538700 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Lactobacillus iners

characteristic: organism

text: The vaginal microbiota plays an important role in the health of the female reproductive tract and is closely associated with a variety of pregnancy outcomes and sexually transmitted diseases. A variety of internal and external factors (such as geography, ethnicity, and diet) have a strong influence on the changes in a woman's vaginal microbiome. The effect of a high-altitude living environment on female vaginal microbiota has not been described. In this study, we characterized the vaginal bacteriome and virome of 13 and 34 healthy women living in high-altitude and sea-level areas, respectively, using whole-metagenome shotgun sequencing of their vaginal mucus samples.

characteristic: project name

characteristic: sample derived from

text: Lactobacillus iners

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus iners

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538700_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538726 of study ERP136557.

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella;s__Klebsiella pneumoniae

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538726_bin.1_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538780 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Enterococcus faecalis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Enterococcus faecalis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Enterococcaceae;g__Enterococcus;s__Enterococcus faecalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538780_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Escherichia coli

text: Escherichia coli

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538780_bin.2_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Atopobium sp.

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: uncultured Atopobium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Atopobium;s__Atopobium vaginae

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538780_bin.3_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538868 of study ERP136557.

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: Gardnerella vaginalis

text: Gardnerella vaginalis

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Gardnerella;s__Gardnerella vaginalis

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538868_bin.1_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR9538876 of study ERP136557.

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: uncultured Porphyromonas sp.

characteristic: organism

text: The vaginal microbiota plays an important role in the health of the female reproductive tract and is closely associated with a variety of pregnancy outcomes and sexually transmitted diseases. A variety of internal and external factors (such as geography, ethnicity, and diet) have a strong influence on the changes in a woman's vaginal microbiome. The effect of a high-altitude living environment on female vaginal microbiota has not been described. In this study, we characterized the vaginal bacteriome and virome of 13 and 34 healthy women living in high-altitude and sea-level areas, respectively, using whole-metagenome shotgun sequencing of their vaginal mucus samples.

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonas sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__Porphyromonas;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538876_bin.1_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Coriobacteriales bacterium

text: uncultured Coriobacteriales bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Coriobacteriia;o__Coriobacteriales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538876_bin.10_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Prevotella timonensis

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Prevotella timonensis

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__Prevotella timonensis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538876_bin.12_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Megasphaera sp.

text: uncultured Megasphaera sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Megasphaera;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538876_bin.6_metaWRAP_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

characteristic: description

text: Vagina

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

characteristic: metagenomic source

text: Aerococcus christensenii

characteristic: organism

characteristic: project name

characteristic: sample derived from

text: Aerococcus christensenii

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Aerococcus;s__Aerococcus christensenii

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR9538876_bin.7_metaWRAP_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: CheckM

text: Vagina

text: China

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

text: uncultured Clostridia bacterium

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR9538876_bin.8_metaWRAP_v1.3_MAG

characteristic: title


E-MTAB-12157:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12157:Sample 1

tag: Namespace:Bernhard Nocht Institute for Tropical Medicine

text: ArrayExpress

text: malaria parasite P. falciparum

characteristic: common name

text: Protocols: Plasmodium falciparum clone 7G8 was cultured at a hematocrit of 5% in human O+ erythrocytes using standard procedures (Trager W & Jensen J 1976. Human malaria parasites in continuous culture. Science 193:673-675. doi: 10.1126/science.781840). To obtain closely synchronized parasites, cell cultures were regularly synchronized with 5% sorbitol (Lambros C & Vanderberg JP 1979. Synchronization of the erythrocytic stages of Plasmodium falciparum in culture. J Parasitol 65:418-420. doi: 10.2307/3280287), with only ring-stage parasites surviving this treatment. Parasites of the 12th in vitro generation were synchronized twice at 8 h intervals to avoid an effect of sorbitol treatment on gene expression at harvest in the following generation. The sample for RNA-seq was obtained by centrifugation of 10 mL of a 1.8% ring-stage P. falciparum cell culture at 800 x g after the next reinvasion. Red cell pellets were rapidly lysed in a 10-fold volume of prewarmed TRIzol containing guanidinium thiocyanate (Life Technologies) and stored at -70°C until RNA purification. RNA was extracted using a silica-based purification protocol using the RNeasy Mini Kit (Qiagen) in conjunction with TRIzol reagent according to the manufacturer's instructions and including DNase treatment on the column. The absence of genomic DNA in the RNA sample was verified by qPCR using the sbp1 primer set (Bachmann A et al. 2016. Mosquito passage dramatically changes var gene expression in controlled human Plasmodium falciparum infections. PLoS Pathog 12(4):e1005538. doi: 10.1371/journal.ppat.1005538). Human globin mRNA was removed from samples using the GLOBINclear kit (Life Technologies) according to the manufacturer's instructions. RNA quantity and quality were assessed using the Qubit RNA HA assay kit with a Qubit 3.0 fluorometer (ThermoFischer Scientific) and the Agilent RNA 6000 Pico kit with an Agilent Bioanalyzer 2100 instrument. A RIN value of 8.4 was obtained. RNA-seq libraries were prepared using the NEBNext Ultra-Directional RNA Library Prep Kit for Illumina. Messenger RNA for RNA-seq libraries were enriched with magnetic beads containing oligo(dT) using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Subsequently, the mRNA was fragmented into short fragments using divalent cations at elevated temperature for 10 min to obtain 325 bp fragments prior to first and second strand cDNA synthesis. Subsequently, double-stranded cDNA was purified using Agencourt AMPure XP beads for final repair and addition of single nucleotide A (adenine) for ligation of sequencing adaptors. Fragments between 100-500 bp (major peak at 325 bp) were selected and USER excision and PCR library enrichment (15 PCR cycles) was performed according to an optimized protocol using KAPA HiFi polymerase in the presence of the PCR additive TMAC to avoid sequencing biases caused by the high content of AT bases of P. falciparum (Oyola SO et al. 2012. Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes. BMC Genomics 13:1. doi: 10.1186/1471-2164-13-1). The final library was quantified in two ways: The average molecular length was determined using the Agilent 2100 Bioanalyzer (Agilent DNA 1000 reagents), and the library was quantified using quantitative real-time PCR (QPCR) (TaqMan probe).

characteristic: description

text: ring stage trophozoite

characteristic: developmental stage

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: Plasmodium falciparum

characteristic: strain

text: Sample 1


E-MTAB-12158:Subclone A1G9

characteristic: External Id

text: Bernhard Nocht Institute for Tropical Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12158:Subclone A1G9

characteristic: Submitter Id

text: ArrayExpress

text: malaria parasite P. falciparum

text: Protocols: The 7G8 subclones were grown at a hematocrit of 5% in human O+ erythrocytes by standard procedures (Trager W & Jensen J 1976. Human malaria parasites in continuous culture. Science 193:673-675. doi: 10.1126/science.781840). Cell cultures were regularly synchronized with 5% sorbitol (Lambros C & Vanderberg JP 1979. Synchronization of the erythrocytic stages of Plasmodium falciparum in culture. J Parasitol 65:418-420. doi: 10.2307/3280287), with only ring stage parasites surviving this treatment. Samples for gDNA-seq were obtained by centrifugation of 150 ml of a P. falciparum cell culture at 800 x g, and red blood cell pellets were lysed in a cell lysis solution containing proteinase K. Genomic DNA was purified according to the manufacturer's instructions \cell samples\ followed by \complete removal of RNA\ with additional RNase I treatment of the MasterPure™ Complete DNA Purification Kit (Lucigen). This included gentle lysis of red blood cells, removal of cell debris and proteins by precipitation, and RNase I treatment in solution, using no phenol, chloroform, or guanidinium salts to avoid interference with downstream NGS sequencing. The gDNA samples were checked for concentration, sample integrity, and purity. Concentration was detected with a fluorometer using the Qubit™ dsDNA BR Assay Kit (ThermoFischer). Sample integrity and purity were detected by agarose gel electrophoresis. Library construction of the gDNA was performed by BGI Genomics Co, Hong Kong. A PCR-free library protocol could be used for the A2E10 and A2G2 subclone samples; the A1G9 subclone sample required a PCR amplification step after adaptor ligation. Per sample, 1 μg of genomic DNA was randomly fragmented by Covaris. The fragmented genome, with an average size of 300-400 bp, was selected with magnetic beads and quantified with a Qubit fluorometer. The fragments were subjected to end repair and then 3'adenylated. Adapters were ligated to the ends of these 3'adenylated fragments. The double-stranded products were heat denatured and circularized through the splint oligo sequence. The single-stranded circular DNA (ssCir DNA) was formatted as the final library. The library was qualified using the Agilent Technologies 2100 Bioanalyzer.

text: asexual blood stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Subclone A1G9


E-MTAB-12158:Subclone A2E10

text: public

text: E-MTAB-12158:Subclone A2E10

text: ArrayExpress

characteristic: broker name

text: malaria parasite P. falciparum

text: asexual blood stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Subclone A2E10


E-MTAB-12158:Subclone A2G2

text: public

text: E-MTAB-12158:Subclone A2G2

text: ArrayExpress

text: malaria parasite P. falciparum

text: asexual blood stage

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: Subclone A2G2

characteristic: title


characteristic: ENA-CHECKLIST

text: Shanghai Tenth People's Hospital

text: public

tag: Namespace:Shanghai Tenth People's Hospital

text: ArrayExpress

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\adenylate, then the adaptors were ligated to the end of these 3\adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

text: wild type genotype

characteristic: genotype

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans

unit: Celsius

characteristic: temperature


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Shanghai Tenth People's Hospital

text: public

characteristic: adapter library

text: ArrayExpress

characteristic: description

text: aneuploid type

text: Candida albicans

text: Candida albicans

text: whole organism

text: Candida albicans


text: Instituto Nacional de Saude Doutor Ricardo Jorge I.P. (INSA) - Portugal

characteristic: CENTER NAME

characteristic: External Id

characteristic: GISAID Accession ID

text: public

tag: Namespace:NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

characteristic: TITLE

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

characteristic: common name

text: Portugal

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: CENTER NAME

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

text: not provided

text: Portugal

text: Human

text: not provided

text: Homo sapiens

text: not provided

text: not provided

characteristic: title


characteristic: External Id

text: Institute for Infectious Diseases

text: public

tag: Namespace:Institute for Infectious Diseases

text: ArrayExpress

characteristic: broker name

text: primary cell line

text: respiratory epithelial cell

text: human

characteristic: common name

text: Protocols: hAEC cultures from 2 different human donors were inoculated in duplicate at the apical surface with 10,000 TCID50 of either the WT or NS1R38A virus or Hank’s Balanced Salt Solution (HBSS) as mock (untreated) control and incubated for 1 hour at 37°C in a humidified incubator with 5% CO2. Afterwards inoculum was removed, and the apical surface was washed three times with HBSS, after which the cells were incubated for an additional 17 hours at 37°C in a humidified incubator with 5% CO2. The apical surface of the remaining inserts was washed four times with 200 µL of HBSS followed by a final washing step of both the apical and basolateral surface with 200 and 500 µL of HBSS, respectively. Cells were dissociated from the Transwell® insert by adding 200 and 500 µL of TrypLE (Thermo Fisher Scientific) to apical and basolateral compartment and an incubation step of 10 minutes at 37°C in a humidified incubator with 5% CO2. This was followed by a gentle disruption of the cell layer through pipetting using a large bore-size pipette tip, and an additional incubation of 20 minutes at 37°C in a humidified incubator with 5% CO2. Dissociated cells were transferred into 800 µL wash solution, which is composed of Air-Liquid Interface (ALI) medium supplemented with 0.1% Pluronic (Thermo Fisher Scientific), and the remaining clumps were gently disrupted through pipetting using a large bore-size pipette tip. Next three cycles of centrifugation for 5 minutes at 250x rcf and resuspension in 1 mL washing solution were performed. Afterward the cells were resuspended in 300 µL washing solution and the cell number, cell viability and cell size were assessed with trypan blue on a Countess II (Thermo Fisher Scientific). Isolation and culturing of primary material was performed as previously described (Jonsdottir J. & Dijkman, R. Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. in Coronaviruses: Methods and Protocols vol. 1282 73-87 (Springer New York, 2015). The hAEC cultures were allowed to differentiate for at least six weeks at 37°C in a humidified incubator with 5% CO2 to ascertain the visual appearance of a well-differentiated pseudostratified layer with ciliary beating and mucus production prior to use. The single cell partitioning was performed on a Chromium Controller (10x Genomics) using the Chromium Single Cell 3' Reagent Kit (version 2, 10x Genomics) according to manufacture protocol. The obtained partitions were further processed using the Chromium Single Cell 3' Reagent Kit (version 2, 10x Genomics) according to manufacture protocol, to generate Nextera XT sequencing libraries.

characteristic: description

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: respiratory system

characteristic: organism part

text: Homo sapiens

text: Mock

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Institute for Infectious Diseases

text: public

text: ArrayExpress

text: primary cell line

text: respiratory epithelial cell

text: human

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: respiratory system

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Institute for Infectious Diseases

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: primary cell line

text: respiratory epithelial cell

text: human

text: normal

text: Homo sapiens

text: Homo sapiens

text: respiratory system

text: Homo sapiens

text: WT


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: NCBI Accession ID

characteristic: SRA accession

characteristic: TITLE

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus

text: Portugal

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Genomes for Australian Plants

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Genomes for Australian Plants

characteristic: Submitter Id

text: Not recorded

characteristic: common name

text: Elachanthus pusillus F.Muell., Asteraceae, Alexander Schmidt-Lebuhn

characteristic: description

text: Australia

text: wild collected

text: Elachanthus pusillus

characteristic: organism

text: Adult

text: wild collected

text: Leaf tissue

text: Elachanthus pusillus

text: CBG 9001681

text: Target capture data for Elachanthus pusillus F.Muell., Bioplatforms Australia Sample ID: 102.100.100/80054 using Angiosperms353 bait kit 10.1093/sysbio/syy086

characteristic: title


text: Genomes for Australian Plants

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Not recorded

text: Isoetopsis graminifolia Turcz., Asteraceae, Alexander Schmidt-Lebuhn

text: Australia

text: wild collected

text: Isoetopsis graminifolia

text: Adult

text: wild collected

text: Leaf tissue

text: Isoetopsis graminifolia

text: CANB 812996

text: Target capture data for Isoetopsis graminifolia Turcz., Bioplatforms Australia Sample ID: 102.100.100/80076 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Barbarea grayi Hewson, Brassicaceae, Brendan Lepschi

text: Australia

text: wild collected

text: Barbarea grayi

text: Adult

text: wild collected

text: Leaf tissue

text: Barbarea grayi

text: CANB 804047

text: Target capture data for Barbarea grayi Hewson, Bioplatforms Australia Sample ID: 102.100.100/80167 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Heliophila pusilla L.f., Brassicaceae, Daniel Murphy

text: Australia

text: wild collected

text: Heliophila pusilla

text: Adult

text: wild collected

text: Leaf tissue

text: Heliophila pusilla

text: MEL 2396290A

text: Target capture data for Heliophila pusilla L.f., Bioplatforms Australia Sample ID: 102.100.100/80639 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Sankowskya stipularis P.I.Forst., Picrodendraceae, Darren Crayn

text: Australia

text: wild collected

text: Sankowskya stipularis

text: Adult

text: wild collected

text: Leaf tissue

text: Sankowskya stipularis

text: CNS 135513

text: Target capture data for Sankowskya stipularis P.I.Forst., Bioplatforms Australia Sample ID: 102.100.100/80960 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Whyanbeelia terrae-reginae Airy Shaw & B.Hyland, Picrodendraceae, Darren Crayn

text: Australia

text: wild collected

text: Whyanbeelia terrae-reginae

text: Adult

text: wild collected

text: Leaf tissue

text: Whyanbeelia terrae-reginae

text: QRS 110338

text: Target capture data for Whyanbeelia terrae-reginae Airy Shaw & B.Hyland, Bioplatforms Australia Sample ID: 102.100.100/81005 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Homalocalyx ericaeus F.Muell., Myrtaceae, Darren Crayn

text: Australia

text: wild collected

text: Homalocalyx ericaeus

text: Adult

text: wild collected

text: Leaf tissue

text: Homalocalyx ericaeus

text: DNA D0274735

text: Target capture data for Homalocalyx ericaeus F.Muell., Bioplatforms Australia Sample ID: 102.100.100/81179 using Angiosperms353 bait kit 10.1093/sysbio/syy086


text: Genomes for Australian Plants

text: public

text: Not recorded

text: Microchloa indica (L.f.) P.Beauv., Poaceae, Darren Crayn

text: Australia

text: wild collected

text: Microchloa indica

text: Adult

text: wild collected

text: Leaf tissue

text: Microchloa indica

text: DNA D0200934

text: Target capture data for Microchloa indica (L.f.) P.Beauv., Bioplatforms Australia Sample ID: 102.100.100/81184 using Angiosperms353 bait kit 10.1093/sysbio/syy086


B

text: public

text: B

tag: Namespace:Seoul National University, College of Medicine

text: Pancreatic epithelial cell

text: human

text: Homo sapiens

text: Homo sapiens

text: pancreas

text: B


text: Child Health Research Foundation

text: public

tag: Namespace:Child Health Research Foundation

text: Salmonella enterica subsp. enterica serovar Paratyphi A


unit: m

characteristic: Depth

text: 2018-06-21T14:20:00Z/2018-06-21T14:35:00Z

characteristic: Event Date/Time

text: Ocean Sampling Day Consortium

text: public

characteristic: Latitude Start

characteristic: Longitude Start

characteristic: Marine Region

characteristic: Protocol Label

unit: practical salinity unit

characteristic: Salinity

characteristic: Sampling Campaign

text: Floating Dock

characteristic: Sampling Platform

text: OSD60, South Carolina North Inlet USA

characteristic: Sampling Site

text: 68354:2ac100af-cb67-4819-8648-122d13563cf9

tag: Namespace:Ocean Sampling Day Consortium

unit: ºC

characteristic: Temperature

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

text: surface water sample from the North Inlet in N. Atlantic

text: sea water [ENVO:00002149]

characteristic: environmental medium

text: water

characteristic: environmental package

text: Prokaryotic samples

text: 2L Nalgene bottles

text: Hand dipped / sumberged under water to fill bottle

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: marine metagenome

text: Ocean Sampling Day

characteristic: project name

text: Sterivex

text: particulate matter on 0.22μm pore size filter

characteristic: sample_filtration time (min)

characteristic: sample_quantity (l)

text: no prefiltration

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

text: water

characteristic: water environmental package


characteristic: Depth

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: 2018-06-21T10:57:00Z/2018-06-21T11:11:00Z

characteristic: Event Date/Time

characteristic: External Id

text: Ocean Sampling Day Consortium

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude Start

characteristic: Longitude Start

characteristic: Marine Region

characteristic: Protocol Label

characteristic: SRA accession

text: off cove by S.C. Dept. of Nature Resources

text: OSD39, Charleston Harbor USA

text: 68348:2ac100af-cb67-4819-8648-122d13563cf9

characteristic: Submitter Id

text: marine biome [ENVO:00000447]

text: GFBIO

text: surface water sample from the Charleston Harbor

characteristic: description

text: sea water [ENVO:00002149]

text: water

text: Prokaryotic samples

text: 2L Nalgene bottles

text: Submerged under surface to fill the bottle by hand

text: microbial community [PCO:1000004]

text: marine metagenome

characteristic: organism

text: Ocean Sampling Day

text: Sterivex

text: no prefiltration

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title

text: water


characteristic: Depth

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: 2018-06-21T09:00:00Z/2018-06-21T17:00:00Z

text: Ocean Sampling Day Consortium

text: public

characteristic: Salinity

characteristic: Sampling Campaign

text: HCMR dinghy boat

characteristic: Sampling Platform

text: OSD5, Crete Greece

characteristic: Sampling Site

text: 68339:2ac100af-cb67-4819-8648-122d13563cf9

characteristic: Temperature

text: marine biome [ENVO:00000447]

characteristic: broad-scale environmental context

text: GFBIO

characteristic: broker name

text: surface water sample from the Cretan Sea

text: sea water [ENVO:00002149]

characteristic: environmental medium

text: water

characteristic: environmental package

text: Niskin bottles / CTD / zooplankton net

text: surface water sampling with niskin 100lt CTD cost / vertical sampling zooplankton

text: microbial community [PCO:1000004]

characteristic: local environmental context

text: marine metagenome

text: Ocean Sampling Day

characteristic: project name

text: Sterivex

text: particulate matter on 47mm 1.2μm pore size polycarbonate filter

text: no prefiltration

text: surface water sampling (100lt), CTD cost, zooplankton net sampling

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc

text: water

characteristic: water environmental package


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Ocean Sampling Day Consortium

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 68335:2ac100af-cb67-4819-8648-122d13563cf9

characteristic: Submitter Id

text: GFBIO

text: PCR blank

characteristic: description

text: marine metagenome

characteristic: organism

text: PCR blank

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Ocean Sampling Day Consortium

text: public

text: 68334:2ac100af-cb67-4819-8648-122d13563cf9

text: GFBIO

characteristic: broker name

text: Extraction blank

text: marine metagenome

text: Extraction blank

text: marine metagenome

text: Illumina MiSeq

text: true


E-MTAB-12124:Sample 1

text: OXFORD

text: public

text: E-MTAB-12124:Sample 1

text: 40 to 85

unit: year

characteristic: age

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: common name

text: Protocols: Libraries were prepared from samples of 74 patients using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus, with additional custom depletion probes, using 100ng RNA. Libraries were sequenced on the Illumina NovaSeqTM 6000 using 100 base paired-end chemistry (108 - 455 million read-pairs per sample). Sequencing reads were processed and aligned to Human Reference genome GRCh38 using the Illumina Dragen RNA pipeline v3.8.4. Libraries were prepared from samples of 74 patients using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus, with additional custom depletion probes, using 100ng RNA. Libraries were sequenced on the Illumina NovaSeqTM 6000 using 100 base paired-end chemistry (108 - 455 million read-pairs per sample). Sequencing reads were processed and aligned to Human Reference genome GRCh38 using the Illumina Dragen RNA pipeline v3.8.4.

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 1


E-MTAB-12124:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 2

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 2


E-MTAB-12124:Sample 4

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 4

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 4


E-MTAB-12124:Sample 6

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 6

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 6

characteristic: title


E-MTAB-12124:Sample 7

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 7

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 7


E-MTAB-12124:Sample 5

text: OXFORD

text: public

text: E-MTAB-12124:Sample 5

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 5


E-MTAB-12124:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 8

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 8


E-MTAB-12124:Sample 9

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 9

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 9


E-MTAB-12124:Sample 3

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 3

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-12124:Sample 10

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 10

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 10


E-MTAB-12124:Sample 11

text: OXFORD

text: public

text: E-MTAB-12124:Sample 11

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 11


E-MTAB-12124:Sample 16

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 16

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 16


E-MTAB-12124:Sample 13

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 13

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 13


E-MTAB-12124:Sample 14

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 14

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 14

characteristic: title


E-MTAB-12124:Sample 22

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 22

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 22


E-MTAB-12124:Sample 12

text: OXFORD

text: public

text: E-MTAB-12124:Sample 12

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 12


E-MTAB-12124:Sample 26

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 26

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 26


E-MTAB-12124:Sample 15

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 15

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 15


E-MTAB-12124:Sample 17

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 17

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 17

characteristic: title


E-MTAB-12124:Sample 18

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 18

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 18


E-MTAB-12124:Sample 35

text: OXFORD

text: public

text: E-MTAB-12124:Sample 35

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 35


E-MTAB-12124:Sample 19

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 19

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 19


E-MTAB-12124:Sample 29

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 29

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 29


E-MTAB-12124:Sample 30

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 30

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 30

characteristic: title


E-MTAB-12124:Sample 23

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 23

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 23


E-MTAB-12124:Sample 20

text: OXFORD

text: public

text: E-MTAB-12124:Sample 20

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 20


E-MTAB-12124:Sample 21

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 21

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 21


E-MTAB-12124:Sample 24

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 24

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 24


E-MTAB-12124:Sample 25

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 25

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 25

characteristic: title


E-MTAB-12124:Sample 27

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 27

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 27


E-MTAB-12124:Sample 28

text: OXFORD

text: public

text: E-MTAB-12124:Sample 28

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 28


E-MTAB-12124:Sample 31

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 31

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 31


E-MTAB-12124:Sample 38

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 38

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 38


E-MTAB-12124:Sample 67

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 67

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 67

characteristic: title


E-MTAB-12124:Sample 33

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 33

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 33


E-MTAB-12124:Sample 34

text: OXFORD

text: public

text: E-MTAB-12124:Sample 34

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 34


E-MTAB-12124:Sample 54

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 54

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 54


E-MTAB-12124:Sample 36

text: OXFORD

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12124:Sample 36

characteristic: Submitter Id

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 36


E-MTAB-12124:Sample 39

characteristic: ENA-CHECKLIST

text: OXFORD

text: public

text: E-MTAB-12124:Sample 39

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

characteristic: description

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 39

characteristic: title


E-MTAB-12124:Sample 32

characteristic: ENA-FIRST-PUBLIC

text: OXFORD

text: public

text: E-MTAB-12124:Sample 32

text: 40 to 85

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mononuclear cell

text: human

characteristic: common name

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 32


E-MTAB-12124:Sample 40

text: OXFORD

text: public

text: E-MTAB-12124:Sample 40

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

characteristic: developmental stage

text: chronic lymphocytic leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: Sample 40


E-MTAB-12124:Sample 37

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: OXFORD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12124:Sample 37

text: 40 to 85

text: ArrayExpress

text: mononuclear cell

text: human

text: adult

text: chronic lymphocytic leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: Sample 37