characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario de Jerez de la Frontera, Jerez de la Frontera, Spain

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00102, Monkeypox virus, WGS, Andalusia Region


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

tag: Namespace:Clinical Bioinformatics Area

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Virgen Macarena, Sevilla, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

unit: DD

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00103, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital de Riotinto, Minas de Riotinto, Spain

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00105, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00107, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00108, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00110, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Infanta Elena, Huelva, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00119, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Reina Sofia, Cordoba, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00121, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00123, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00124, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00126, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario de Jerez de la Frontera, Jerez de la Frontera, Spain

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00128, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Virgen Macarena, Sevilla, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: active surveillance in response to outbreak

text: Monkeypox virus

text: ANDmpxv00130, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00132, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00133, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00135, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00136, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00137, Monkeypox virus, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00138, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: organism

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00139, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Health Board of Estonia

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli v0.6.0 @ Galaxy

tag: Namespace:Health Board of Estonia

text: The Health Board of Estonia

text: Aare Abroi, Radko Avi, Taavi Päll, Heiki Niglas , Merit Pauskar, Arina Shablinskaja, Eveli Kallas, Andrio Lahesaare, Kai Truusalu, Dagmar Hoidmets, Olga Sadikova, Kaspar Ratnik, Hanna Sepp, Liidia Dotsenko, Jevgenia Epštein, Heleene Suija, Katrin Kaarna, Lili Milani, Mait Metspalu, Ott Eric Oopkaup, Ivar Koppel, Erik Jaaniso, Ivan Kuzmin, Uku Raudvere, Tuuli Reisberg, Hedi Peterson, Ulvi Gerst Talas, Paul Naaber, Mari-Anne Härma, Irja Lutsar, Kristi Huik, Heleri Inno

characteristic: collector name

text: Nasopharyngeal/oropharyngeal swab from Estonian Covid-19 patient

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Northern Estonia

characteristic: geographic location (region and locality)

unit: year

characteristic: host age

text: human

characteristic: host common name

text: diseased

text: Homo sapiens

text: male

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance not initiated by an outbreak

text: PCR tiled amplicon WGS of SARS-Cov-2, pre-selected subset from active surveillance


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Health Board of Estonia

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli v0.6.0 @ Galaxy

characteristic: Submitter Id

text: The Health Board of Estonia

characteristic: collecting institution

characteristic: description

text: Estonia

text: North-Eastern Estonia

text: human

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex


characteristic: External Id

text: CENTRE FOR GEOGENETICS

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:CENTRE FOR GEOGENETICS

text: environmental samples

characteristic: organism

text: environmental samples

characteristic: title


characteristic: ENA-CHECKLIST

text: UPSC

text: public

text: Norway spruce

characteristic: common name

text: Female cone of Wild-type genotype sampled on 08-Oct 2013 from tree number 1

text: Picea abies

text: Picea abies


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UPSC

text: public

text: Norway spruce

text: Female cone of Wild-type genotype sampled on 08-Oct 2013 from tree number 2

text: Picea abies

text: Picea abies


text: UPSC

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: Norway spruce

text: Female cone of Wild-type genotype sampled on 08-Oct 2013 from tree number 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

characteristic: Submitter Id

text: Norway spruce

text: Female cone of Wild-type genotype sampled on 08-Oct 2013 from tree number 4

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Female cone of Wild-type genotype sampled on 08-Oct 2013 from tree number 5

characteristic: description

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Vegetative primordia of Wild-type genotype sampled on 08-Oct 2013 from tree number 1

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Vegetative primordia of Wild-type genotype sampled on 08-Oct 2013 from tree number 2

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Vegetative primordia of Wild-type genotype sampled on 08-Oct 2013 from tree number 3

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Vegetative primordia of Wild-type genotype sampled on 08-Oct 2013 from tree number 4

text: Picea abies

text: Picea abies


text: UPSC

text: public

text: Norway spruce

text: Vegetative primordia of Wild-type genotype sampled on 08-Oct 2013 from tree number 5

text: Picea abies

text: Picea abies


text: STELLENBOSCH UNIVERSITY

text: public

tag: Namespace:STELLENBOSCH UNIVERSITY

characteristic: collection date

text: progenitor

text: South Africa

characteristic: geographic location (country and/or sea)

text: Homo Sapiens

text: standard culture

characteristic: isolate

text: standard culture

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis H37Rv lab strain


characteristic: External Id

text: STELLENBOSCH UNIVERSITY

text: public

text: point mutant: atpE_colony 14

text: South Africa

text: Homo Sapiens

characteristic: host scientific name

text: standard culture

text: standard culture

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis modified H37Rv lab strain (point mutant: atpE_colony 14)


text: STELLENBOSCH UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: point mutant: rv0678 _colony 15

text: South Africa

text: Homo Sapiens

text: standard culture

text: standard culture

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis modified H37Rv lab strain (point mutant: rv0678 _colony 15)


text: STELLENBOSCH UNIVERSITY

text: public

characteristic: INSDC status

text: point mutant: rv0678_ colony 46

text: South Africa

text: Homo Sapiens

text: standard culture

text: standard culture

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis modified H37Rv lab strain (point mutant: rv0678_ colony 46)


characteristic: ENA-CHECKLIST

text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

tag: Namespace:Hospital Ramon y Cajal-IRYCIS-CIBERER

text: human

text: Elite controller 1

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

characteristic: common name

text: Elite controller 2

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: Hospital Ramon y Cajal-IRYCIS-CIBERER

characteristic: INSDC first public

text: public

text: human

text: Elite controller 3

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Elite controller 4

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Elite controller 5

characteristic: description

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Elite controller 6

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 1

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 3

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 4

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 5

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 6

text: Homo sapiens

text: Homo sapiens


text: Hospital Ramon y Cajal-IRYCIS-CIBERER

text: public

text: human

text: Progressor 7

text: Homo sapiens

text: Homo sapiens


E-MTAB-12027:Sample 1

text: public

text: E-MTAB-12027:Sample 1

tag: Namespace:Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury

text: ArrayExpress

characteristic: broker name

text: pig

text: Protocols: The procedure for collection and incubation of the porcine CL was previously described [20]. In the laboratory, the CL was separated from the ovary, cleaned and placed on ice in a sterile Petri dish. CLs were cut into small pieces (100 ± 10 mg, in duplicate from each experimental replicate). Each tissue explant was placed in M199 medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 0.1% BSA fraction V (Roth, Germany) and antibiotics. The explants were pre-incubated for 2 h in a water bath at 37°C in an atmosphere of 95% O2 and 5% CO2. Then, the explants were treated with LPS (100 ng/ml, from Escherichia coli) for 24 h. Explants not treated with LPS were considered as controls. The medium was removed, and the explants were incubated for 6 h with LPS alone or in combination with PPAR β/δ ligands: GW0724 (agonist; 1 μmol/L or 10 μmol/L, Cayman Chemical Company) or GSK3787 (antagonist; 25 μmol/L, Cayman Chemical Company). Controls also contained dimethyl sulfoxide (DMSO, solvent for the tested PPAR ligands). After the incubation, tissue explants were frozen at -80°C until further analysis. Total RNA from 16 samples was isolated using the \RNeasy Mini Kit\ (Qiagen, Germany) according to the manufacturer's protocol. The Tecan Infinite M200 plate reader (Tecan Group Ltd., Switzerland) and Agilent Bioanalyzer 2100 (Agilent Technology, USA) were used to evaluate total RNA quantity and quality. The samples with RNA Integrity Number (RIN) > 7 were selected for the next analyses. The poly(A) RNA-sequencing library was prepared according to the Illumina TruSeq Stranded mRNA Sample Preparation Protocol. Two rounds of purification were performed using oligo(dT) magnetic beads to purify the poly(A) tailed mRNA. Subsequently, the poly(A) RNA was fragmented at high temperature using a divalent cation buffer, and poly(dT) oligonucleotides were used to transcribe the RNA into cDNA. Subsequently, the cDNA was subjected to 3' tail adenylation and adapter ligation. Reverse transcription during library construction was strand-specific

text: explant culture

characteristic: growth condition

text: Sus scrofa

text: Sus scrofa

text: ovary

characteristic: organism part

text: corpus luteum

characteristic: sampling site

text: mid-luteal phase

characteristic: sampling time point

text: Sus scrofa

text: Sample 1


E-MTAB-12027:Sample 2

characteristic: External Id

text: Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: E-MTAB-12027:Sample 2

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 2


E-MTAB-12027:Sample 3

characteristic: ENA-CHECKLIST

text: public

characteristic: INSDC status

text: E-MTAB-12027:Sample 3

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 3


E-MTAB-12027:Sample 4

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12027:Sample 4

text: ArrayExpress

text: pig

characteristic: common name

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 4

characteristic: title


E-MTAB-12027:Sample 17

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12027:Sample 17

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 17


E-MTAB-12027:Sample 18

text: public

text: E-MTAB-12027:Sample 18

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 18


E-MTAB-12027:Sample 19

text: public

text: E-MTAB-12027:Sample 19

text: ArrayExpress

text: pig

text: explant culture

characteristic: growth condition

text: Sus scrofa

text: Sus scrofa

text: ovary

characteristic: organism part

text: corpus luteum

characteristic: sampling site

text: mid-luteal phase

text: Sus scrofa

text: Sample 19


E-MTAB-12027:Sample 20

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12027:Sample 20

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

characteristic: sampling time point

text: Sus scrofa

text: Sample 20


E-MTAB-12027:Sample 9

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12027:Sample 9

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 9


E-MTAB-12027:Sample 10

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-12027:Sample 10

text: ArrayExpress

text: pig

characteristic: common name

text: explant culture

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 10


E-MTAB-12027:Sample 11

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: E-MTAB-12027:Sample 11

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 11

characteristic: title


E-MTAB-12027:Sample 12

text: public

characteristic: SRA accession

text: E-MTAB-12027:Sample 12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 12


E-MTAB-12027:Sample 13

text: public

text: E-MTAB-12027:Sample 13

text: ArrayExpress

text: pig

characteristic: description

text: explant culture

characteristic: growth condition

text: Sus scrofa

text: Sus scrofa

text: ovary

characteristic: organism part

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 13


E-MTAB-12027:Sample 14

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12027:Sample 14

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

characteristic: sampling site

text: mid-luteal phase

text: Sus scrofa

text: Sample 14


E-MTAB-12027:Sample 15

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

text: E-MTAB-12027:Sample 15

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

characteristic: sampling time point

text: Sus scrofa

text: Sample 15


E-MTAB-12027:Sample 16

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: E-MTAB-12027:Sample 16

text: ArrayExpress

text: pig

characteristic: common name

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 16


E-MTAB-12027:Sample 5

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

text: public

text: E-MTAB-12027:Sample 5

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

characteristic: organism

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 5


E-MTAB-12027:Sample 6

text: public

characteristic: SRA accession

text: E-MTAB-12027:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 6

characteristic: title


E-MTAB-12027:Sample 7

text: public

text: E-MTAB-12027:Sample 7

text: ArrayExpress

characteristic: broker name

text: pig

characteristic: description

text: explant culture

characteristic: growth condition

text: Sus scrofa

text: Sus scrofa

text: ovary

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 7


E-MTAB-12027:Sample 8

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-12027:Sample 8

text: ArrayExpress

text: pig

text: explant culture

text: Sus scrofa

text: Sus scrofa

text: ovary

characteristic: organism part

text: corpus luteum

text: mid-luteal phase

text: Sus scrofa

text: Sample 8


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Infanta Elena, Huelva, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00140, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Juan Ramon Jimenez, Huelva, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00141, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00142, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario de Puerto Real, Puerto Real, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00143, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Infanta Margarita, Cabra, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00145, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Virgen de Valme, Sevilla, Spain

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00146, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital de La Línea de La Concepción, Cádiz, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00147, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00148, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00149, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Punta de Europa, Algeciras, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00150, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Universitario Virgen del Rocio, Sevilla, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: active surveillance in response to outbreak

text: Monkeypox virus

text: ANDmpxv00151, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00152, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00153, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00154, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00155, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00156, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00157, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: Servicio de Urgencias, Hospital Santa Ana, Motril, Spain

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00159, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Complejo Hospitalario Torrecardenas, Almeria, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00161, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Virgen de la Victoria, Malaga, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00162, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00163, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ena-upload-cli

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00164, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital de Poniente, El Ejido, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00165, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital de Antequera, Antequera, Spain

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: active surveillance in response to outbreak

text: Monkeypox virus

text: ANDmpxv00167, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Universitario San Cecilio, Granada, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00169, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00171, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00172, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Costa del Sol, Marbella, Spain

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Monkeypox virus

characteristic: sample capture status

text: Monkeypox virus

text: ANDmpxv00175, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

text: Clinical Bioinformatics Area

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

text: Monkeypox virus, WGS, Andalusia Region

characteristic: description

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

characteristic: organism

text: Monkeypox virus

text: ANDmpxv00177, Monkeypox virus, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Monkeypox virus, WGS, Andalusia Region

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: Monkeypox virus

text: Monkeypox virus

text: ANDmpxv00178, Monkeypox virus, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Liverpool, Institute of Infection, Veterinary and Ecological sciences

text: The Group for Enteric respiratory and meningeal disease surveillance - South Africa

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

characteristic: geographic location (region and locality)

text: Homo sapien

characteristic: host scientific name

text: Stool culture

characteristic: isolate

text: Stool culture

characteristic: isolation source host-associated

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Liverpool, Institute of Infection, Veterinary and Ecological sciences

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: South African Shigella flexneri 2a

characteristic: description

text: South Africa

text: Eastern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: Shigella flexneri 2a

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: South African Shigella flexneri 2a

text: South Africa

characteristic: geographic location (country and/or sea)

text: Mpumalanga

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: South African Shigella flexneri 2a

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Western Cape

characteristic: geographic location (region and locality)

text: Homo sapien

characteristic: host scientific name

text: Stool culture

characteristic: isolate

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

text: Homo sapien

text: Stool culture

text: Stool culture

characteristic: isolation source host-associated

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Free State

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


text: public

characteristic: Submitter Id

text: South African Shigella flexneri 2a

characteristic: description

text: South Africa

text: Northern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Fluid

text: Fluid

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a

characteristic: title


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: South African Shigella sonnei

text: South Africa

characteristic: geographic location (country and/or sea)

text: Western Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


text: public

characteristic: SRA accession

text: South African Shigella sonnei

text: South Africa

text: Gauteng

characteristic: geographic location (region and locality)

text: Homo sapien

characteristic: host scientific name

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella sonnei

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Stool culture

characteristic: isolate

text: Stool culture

characteristic: isolation source host-associated

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: South African Shigella sonnei

text: South Africa

text: Eastern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: South African Shigella sonnei

text: South Africa

text: Free State

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


text: public

text: South African Shigella sonnei

characteristic: description

text: South Africa

text: Mpumalanga

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

characteristic: organism

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: South African Shigella sonnei

text: South Africa

text: Gauteng

text: Homo sapien

text: Blood culture

text: Blood culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: INSDC last update

text: public

text: South African Shigella sonnei

text: South Africa

text: North West

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei

characteristic: title


text: public

characteristic: INSDC status

text: South African Shigella sonnei

text: South Africa

characteristic: geographic location (country and/or sea)

text: Gauteng

text: Homo sapien

text: Urine culture

text: Urine culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


text: public

characteristic: SRA accession

text: South African Shigella sonnei

text: South Africa

text: Northern Cape

characteristic: geographic location (region and locality)

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: North West

text: Homo sapien

characteristic: host scientific name

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


characteristic: ENA-LAST-UPDATE

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Stool culture

characteristic: isolate

text: Stool culture

characteristic: isolation source host-associated

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: South African Shigella flexneri 2a

text: South Africa

text: Western Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-CHECKLIST

text: public

text: South African Shigella flexneri 2a

characteristic: description

text: South Africa

text: Eastern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

text: South African Shigella flexneri 2a

text: South Africa

characteristic: geographic location (country and/or sea)

text: Free State

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

characteristic: geographic location (region and locality)

text: Homo sapien

characteristic: host scientific name

text: Blood culture

text: Blood culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-LAST-UPDATE

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Western Cape

text: Homo sapien

text: Blood culture

characteristic: isolate

text: Blood culture

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


characteristic: External Id

characteristic: INSDC center name

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Limpopo

text: Homo sapien

text: Stool culture

text: Stool culture

characteristic: isolation source host-associated

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-CHECKLIST

text: public

characteristic: Submitter Id

text: South African Shigella sonnei

characteristic: description

text: South Africa

text: Western Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: South African Shigella sonnei

text: South Africa

text: Gauteng

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

characteristic: organism

text: Shigella sonnei

text: Shigella sonnei


text: public

characteristic: INSDC status

characteristic: SRA accession

text: South African Shigella sonnei

text: South Africa

text: Eastern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella sonnei

text: South Africa

characteristic: geographic location (country and/or sea)

text: KwaZulu-Natal

characteristic: geographic location (region and locality)

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-LAST-UPDATE

text: public

text: South African Shigella sonnei

text: South Africa

text: Mpumalanga

text: Homo sapien

characteristic: host scientific name

text: Stool culture

characteristic: isolate

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: External Id

characteristic: INSDC center name

text: public

text: South African Shigella sonnei

text: South Africa

text: North West

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


characteristic: ENA-CHECKLIST

text: public

text: South African Shigella sonnei

text: South Africa

text: Free State

text: Homo sapien

text: Stool culture

text: Stool culture

characteristic: isolation source host-associated

text: Shigella sonnei

text: Shigella sonnei

text: Shigella sonnei


text: public

characteristic: Submitter Id

text: South African Shigella flexneri 2a

characteristic: description

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Blood culture

text: Blood culture

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Northern Cape

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: Shigella flexneri 2a


text: public

characteristic: INSDC status

characteristic: SRA accession

text: South African Shigella flexneri 2a

text: South Africa

text: Mpumalanga

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella flexneri 2a

text: South Africa

characteristic: geographic location (country and/or sea)

text: Western Cape

characteristic: geographic location (region and locality)

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-LAST-UPDATE

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Eastern Cape

text: Homo sapien

characteristic: host scientific name

text: Stool culture

characteristic: isolate

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


text: public

characteristic: Submitter Id

text: South African Shigella flexneri 2a

characteristic: description

text: South Africa

text: KwaZulu-Natal

text: Homo sapien

text: Stool culture

text: Stool culture

characteristic: isolation source host-associated

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: South African Shigella flexneri 2a

text: South Africa

text: Free State

text: Homo sapien

text: Stool culture

text: Stool culture

text: Shigella flexneri

text: Shigella flexneri

characteristic: serovar

text: Shigella flexneri 2a


text: public

characteristic: INSDC status

characteristic: SRA accession

text: South African Shigella flexneri 2a

text: South Africa

text: Gauteng

text: Homo sapien

text: Fluid

text: Fluid

text: Shigella flexneri

characteristic: organism

text: Shigella flexneri

text: Shigella flexneri 2a


characteristic: ENA-FIRST-PUBLIC

text: public

text: South African Shigella flexneri 2a

text: South Africa

characteristic: geographic location (country and/or sea)

text: Western Cape

text: Homo sapien

text: Fluid

text: Fluid

text: Shigella flexneri

text: Shigella flexneri

text: Shigella flexneri 2a

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Fondazione Pisana per la Scienza ONLUS

text: public

tag: Namespace:Fondazione Pisana per la Scienza ONLUS

text: human

characteristic: common name

text: Tissue fragment of glioblastoma

text: Homo sapiens

characteristic: patient age at collection of tumor

text: Male

characteristic: patient sex

characteristic: patient tumor diagnosis at time of collection

characteristic: patient tumor primary site

characteristic: patient tumor site of collection

text: Primary Neoplasm

characteristic: patient tumor type

text: tissue fragment

characteristic: sample material

text: Patient tumor

characteristic: sample origin

text: Homo sapiens

characteristic: sample taxon name

characteristic: sample unique ID

text: Homo sapiens

text: Homo Sapiens Brain Tissue


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Fondazione Pisana per la Scienza ONLUS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Tissue fragment of glioblastoma

characteristic: description

text: Homo sapiens

text: Female

text: Primary Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens Brain Tissue


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, DBSSE

text: public

tag: Namespace:ETH Zurich, DBSSE

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: Cerebral organoids were generated using a whole brain organoid differentiation protocol (Lancaster et al. 2013 https://doi.org/10.1038/nature12517). To generate Cas9 mediated GLI3 KO cell lines, two days prior to lipofection, iPSC media was supplemented with 2 µg/ml doxycycline (Clontech, 631311) to induce Cas9n expression. Two guides were designed using the Broad Institute’s CRISPR design tool (http://crispr.mit.edu/). The following guide pair was selected: ACAGAGGGCTCCGCCACGTGTGG, CCGCGGGACGGTGTTTGCCATGG. The Alt-R CRISPR-Cas9 System (IDT) was used for guide delivery with lipofection according to the manufacturer's protocol. To form the crRNA:tracrRNA complex in a 3 µM final concentration for each guide complex, 1.5 µl of each guide crRNA was combined with 3 µl tracrRNA and 44 µl nuclease-free water. For the reverse transfection, 1.5µl of the crRNA:tracr complex mix and 0.75 µl RNAiMAX (Invitrogen, 13778075) were diluted in 47.75 µl OPTI-MEM (Gibco, 1985-062) for each replicate and incubated for 20 minutes at RT in a well of 96-well plate coated with Matrigel (Corning, 35248). During incubation, ~70% confluent cells were detached with TryplE (Gibco, 12605010), centrifuged and resuspended in 1ml mTeSR with Y-27632 (final concentration 10µM, STEMCELL, 72302). After complex incubation, cells were diluted 30 or 60 times in 100 µl mTeSR with Y-27632 (STEMCELL, 72302) and 2 µg/ml doxycycline (Clontech, 631311) and the cell suspension was added to a well containing the transfection complexes. After 24h media was replaced with mTeSR1 media and cells were allowed to recover for 72h. 70% confluent wells were used for further processing after 72 hours. Cells were passaged as single cells in a Matrigel-coated (Corning, 35248) 6-well plate in mTeSR media with 1:200 P/S (Gibco, 15140122) and Y-27632 (STEMCELL, 72302). Low amounts of cells were plated per well to avoid the fusion of colonies. Media was changed daily and Y-27632 was added for the first 72 hours to prevent apoptosis of the single cells. When colonies were apparent, single colonies were picked by scraping with a 10 microliter pipette tip. 2/3 of the cell suspension was plated in a single well of a Matrigel-coated 96-well plate in mTeSR1 supplemented with 1:200 P/S and Y-27632. The other portion of the cell suspension was pelleted and used for validation of frameshift mutations by sequencing. Validated clones were expanded, cryopreserved and karyotyped. Single cell suspensions were acquired by dissociation of the organoids with a papain-based dissociation (Miltenyi Biotec, 130-092-628). Nuclei were isolated according to the protocol provided by 10x genomics (Demonstrated protocol CG000365, Rev B) with a lysis time of 3 min. Combined scRNA-seq and scATAC-seq libraries were generated with the Chromium Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer's protocol. Combined scRNA-seq and scATAC-seq libraries were generated with the Chromium Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer's protocol.

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: CRISPR Cas9 mediated GLI3 knockout (4 and 8 bp insertion)

characteristic: genetic modification

text: inducible Cas9 nuclease

characteristic: genotype

text: cerebral organoid culture

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: dermis

characteristic: organism part

text: induced pluripotent stem cell

characteristic: progenitor cell type

text: Homo sapiens

text: female

characteristic: sex

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: ETH Zurich, DBSSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

text: adult

text: normal

text: CRISPR Cas9 mediated GLI3 knockout (29 and 41 bp deletion)

text: inducible Cas9 nuclease

text: cerebral organoid culture

text: Homo sapiens

text: Homo sapiens

text: dermis

text: induced pluripotent stem cell

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, DBSSE

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: inducible Cas9 nuclease

text: cerebral organoid culture

text: Homo sapiens

text: Homo sapiens

text: dermis

text: induced pluripotent stem cell

text: Homo sapiens

text: female


text: Computational Biology Unit

text: public

text: ena-SAMPLE-TAB-08-08-2022-11:55:36:722-29722

tag: Namespace:Computational Biology Unit

text: zebrafish

text: RNA-seq Wild type Male 1

text: Danio rerio

characteristic: organism

text: Danio rerio

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: Male

characteristic: sex

text: Kidney

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Computational Biology Unit

characteristic: INSDC center name

characteristic: INSDC first public

text: public

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