characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Pseudomonadales bacterium

characteristic: organism

text: uncultured Pseudomonadales bacterium

text: PacBio Sequel II

text: Multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin20


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

text: public

tag: Namespace:MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

text: Vamb was run in three different modes with the following parameters: set1 -l 32 -n 512 512, set2 -l 24 -n 384 384 and set3 -l 40 -n 768 768

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624915,ERR9575721

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured SAR86 cluster bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured SAR86 cluster bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin21

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624918,ERR9575718

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured SAR86 cluster bacterium

characteristic: organism

text: uncultured SAR86 cluster bacterium

text: PacBio Sequel II

text: Multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin22


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: SAR92 clade bacterium

characteristic: project name

characteristic: sample derived from

text: SAR92 clade bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin23

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624913,ERR9575723

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Ulvibacter sp.

characteristic: organism

text: uncultured Ulvibacter sp.

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin24


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624917,ERR9575719

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Verrucomicrobiae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Verrucomicrobiae bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin25

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Hyphomicrobiales bacterium

characteristic: organism

text: uncultured Hyphomicrobiales bacterium

text: PacBio Sequel II

text: Multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin26


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624916,ERR9575720

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: Rhodococcus erythropolis

characteristic: project name

characteristic: sample derived from

text: Rhodococcus erythropolis

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin27

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Flavobacteriaceae bacterium

characteristic: organism

text: uncultured Flavobacteriaceae bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin28


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: SAR92 clade bacterium

characteristic: project name

characteristic: sample derived from

text: SAR92 clade bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin29

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Alphaproteobacteria bacterium

characteristic: organism

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin3


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

text: Vamb was run in three different modes with the following parameters: set1 -l 32 -n 512 512, set2 -l 24 -n 384 384 and set3 -l 40 -n 768 768

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Flavobacteriales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Flavobacteriales bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin30

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Opitutales bacterium

characteristic: organism

text: uncultured Opitutales bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin31


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Pseudomonadales bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudomonadales bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin32

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624914,ERR9575722

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured OM182 clade bacterium

characteristic: organism

text: uncultured OM182 clade bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin33


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured OM182 clade bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured OM182 clade bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin34

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured OM182 clade bacterium

characteristic: organism

text: uncultured OM182 clade bacterium

text: PacBio Sequel II

text: Multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin35


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: Akkermansiaceae bacterium

characteristic: project name

characteristic: sample derived from

text: Akkermansiaceae bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin36

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624913,ERR9575723

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Polaribacter sp.

characteristic: organism

text: uncultured Polaribacter sp.

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin37


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624915,ERR9575721

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Verrucomicrobiae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Verrucomicrobiae bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin38

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624911,ERR9575725

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured SAR86 cluster bacterium

characteristic: organism

text: uncultured SAR86 cluster bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin39


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

text: Vamb was run in three different modes with the following parameters: set1 -l 32 -n 512 512, set2 -l 24 -n 384 384 and set3 -l 40 -n 768 768

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Gammaproteobacteria bacterium

characteristic: project name

characteristic: sample derived from

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin4

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Verrucomicrobiae bacterium

characteristic: organism

text: uncultured Verrucomicrobiae bacterium

text: PacBio Sequel II

text: Multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin40


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: uncultured Glaciecola sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Glaciecola sp.

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin41

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624914,ERR9575722

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: Akkermansiaceae bacterium

characteristic: organism

text: Akkermansiaceae bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin42


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

characteristic: project name

characteristic: sample derived from

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin6

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: uncultured Nitrospinaceae bacterium

characteristic: organism

text: uncultured Nitrospinaceae bacterium

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin7


characteristic: ENA-CHECKLIST

text: public

characteristic: assembly quality

text: Flye v2.8.3

characteristic: assembly software

characteristic: binning parameters

text: Vamb v3.0.2

characteristic: binning software

characteristic: completeness score

text: CheckM v1.0.13

characteristic: completeness software

characteristic: contamination score

text: marine biome [ENVO:00000447]

text: marine photic zone [ENVO:00000209]

text: sea water [ENVO:00002149]

text: Arctic Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: sea water

text: marine metagenome

characteristic: metagenomic source

text: Vicinamibacterales bacterium

characteristic: project name

characteristic: sample derived from

text: Vicinamibacterales bacterium

text: PacBio Sequel II

characteristic: sequencing method

text: 16S gene and multi-marker approach

characteristic: taxonomic identity marker

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin8

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Flye v2.8.3

text: Vamb v3.0.2

characteristic: collection date

text: CheckM v1.0.13

text: This sample represents a manually-refined metagenome-assembled genome recovered from a PacBio HiFi read metagenome of a water column sample in the Fram Strait. The metagenome sample and related assembly can be found under ERS11624918,ERR9575718

characteristic: description

text: marine biome [ENVO:00000447]

characteristic: environment (biome)

text: marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: sea water [ENVO:00002149]

characteristic: environment (material)

text: Arctic Ocean

characteristic: geographic location (country and/or sea)

text: metagenome

text: sea water

text: marine metagenome

text: Marinomonas primoryensis

characteristic: organism

text: Marinomonas primoryensis

text: PacBio Sequel II

text: 16S gene and multi-marker approach

text: Fram Strait water column MAG - FRAM_RAS_EGC1617_bin9


characteristic: ENA-CHECKLIST

text: University of Zurich

text: public

tag: Namespace:University of Zurich

text: Switzerland

text: not collected

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: clinical sample

text: not collected

text: Campylobacter jejuni

text: Campylobacter jejuni

text: Whole genome-based characterization of Campylobacter jejuni Switzerland

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Liverpool School Of Tropical Medicine

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Liverpool School Of Tropical Medicine

text: African malaria mosquito

characteristic: common name

text: Ten female mosquitoes from Ghana surviving 0.1% Bendiocarb treatment

text: Anopheles funestus

text: Anopheles funestus


text: Liverpool School Of Tropical Medicine

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: African malaria mosquito

text: Ten female mosquitoes from Malawi surviving 0.1% Bendiocarb treatment

characteristic: description

text: Anopheles funestus

text: Anopheles funestus


text: public

tag: Namespace:Department of Ecology and Genetics, Uppsala University

text: Offspring

text: Vanessa cardui

characteristic: organism

text: Vanessa cardui

text: RAD-seq


characteristic: ENA-CHECKLIST

text: Department of Ecology and Genetics, Uppsala University

text: public

text: Offspring

text: Vanessa cardui

text: Vanessa cardui

text: RAD-seq


text: public

text: Dam

text: Vanessa cardui

text: Vanessa cardui

text: RAD-seq


text: Tromsoe Ecosystem Genomics

text: public

tag: Namespace:Tromsoe Ecosystem Genomics

text: Sulsseewli GH sequence library 1 (ECOG-1-7)

text: Viridiplantae environmental sample

text: Viridiplantae environmental sample


text: Tromsoe Ecosystem Genomics

text: public

text: Sulsseewli GH sequence library 2 (ECOG-1-8)

text: Viridiplantae environmental sample

text: Viridiplantae environmental sample


text: Tromsoe Ecosystem Genomics

text: public

text: Sulsseewli 16S sequence library 1 (TEG-012 - mam1_EG33_16S)

text: Metazoa environmental sample

text: Metazoa environmental sample


text: Tromsoe Ecosystem Genomics

text: public

text: Sulsseewli 16S sequence library 2 (TEG-013 - EG33_mam)

text: Metazoa environmental sample

text: Metazoa environmental sample


E-MTAB-11626:Sample 3

text: public

text: E-MTAB-11626:Sample 3

tag: Namespace:Institut Necker Enfants Malades (INEM), INSERM U1151/CNRS UMS 8253, Universite de Paris, Paris, France.

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mixed

text: human

text: Protocols: All spleen in this study were collected from organ donors who died from stroke or head trauma. None of them had lymphoma or autoimmune disease. Immediately after splenectomy, mononuclear cells were isolated from crushed pieces of splenic tissue by ficoll density-gradient centrifugation and stored in liquid nitrogen. After thawing and subsequent apoptotic cell removal step using the Dead Cell Removal kit (Miltenyi), mononuclear cells were counted and stained with antibodies directed against surface markers. Splenocytes were first enriched for IgG+CD3-CD14-CD16+ cells from which B5R-specific and total IgG+ switched memory B cells (B5R+ or B5R-IgG+CD27+CD20+IgD-) were further single-cell FACS-sorted (AriaIII) into 384-well plates containing 384 primers and Mineral oil (Sigma). Naives B cells were sorted from the IgG-CD3-CD14-CD16+ fraction as CD20+CD38lowCD24intCD27-IgD+. After sorting, plates were snap-frozen on dry ice and stored at -80° C. The “well id”, \cell type\, “additional cell attribute” and “disease” attributes in plate layouts recapitulate all sorting information for each cell. Index-sorting information and parallel in vitro validation using single culture were additionally use to optimized gating strategies and remove potential sort contaminants from further analysis in the context of IgG+ B5R+ memory B cell sorts (“indexsorting.validation” attributes in plate layouts). This dataset was performed in two distincts batches: batch 1 [Samples: EW01 and EW02] and batch 2 [Samples: CW01 to CW10]. Each sample corresponds to a sorted 384 plate containing cells from one donor from all major populations to be compared. “group”, “individual”, “age” and “sex” attributes in plate layouts recapitulate information for each sorted donor as they appear in the original publication. “sort id” attribute indicate unique sort sessions. All plates from each of the two batches, regardless of the sorting date, were processed at the same time for library preparation and sequencing. For amplification, cells were heat-lysed at 65°C followed by cDNA synthesis using the CEL-Seq2 protocol (T. Hashimshony, N. Senderovich, G. Avital, A. Klochendler, Y. de Leeuw, L. Anavy, D. Gennert, S. Li, K. J. Livak, O. Rozenblatt-Rosen, Y. Dor, A. Regev, I. Yanai, CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq, Genome Biol. 17, 77 (2016)) and robotic liquid handling platforms. After second strand cDNA synthesis, the barcoded material was pooled into libraries of 384 cells and amplified using IVT. Following amplification, the rest of the CEL-seq2 protocol (T. Hashimshony, N. Senderovich, G. Avital, A. Klochendler, Y. de Leeuw, L. Anavy, D. Gennert, S. Li, K. J. Livak, O. Rozenblatt-Rosen, Y. Dor, A. Regev, I. Yanai, CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq, Genome Biol. 17, 77 (2016)) was followed for preparation of the amplified cDNA library, using TruSeq small RNA primers (Illumina).

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

characteristic: plate

text: Homo sapiens

text: female

characteristic: sex

text: Sample 3


E-MTAB-11626:Sample 4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut Necker Enfants Malades (INEM), INSERM U1151/CNRS UMS 8253, Universite de Paris, Paris, France.

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11626:Sample 4

text: ArrayExpress

text: mixed

text: human

characteristic: common name

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

text: Sample 4

characteristic: title


E-MTAB-11626:Sample 5

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11626:Sample 5

characteristic: Submitter Id

text: ArrayExpress

text: mixed

text: human

characteristic: description

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

text: Sample 5


E-MTAB-11626:Sample 6

text: public

text: E-MTAB-11626:Sample 6

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mixed

text: human

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 6


E-MTAB-11626:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11626:Sample 7

text: ArrayExpress

text: mixed

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: plate

text: Homo sapiens

text: male

characteristic: sex

text: Sample 7


E-MTAB-11626:Sample 8

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11626:Sample 8

characteristic: Submitter Id

text: ArrayExpress

text: mixed

text: human

characteristic: common name

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

text: Sample 8

characteristic: title


E-MTAB-11626:Sample 9

text: public

text: E-MTAB-11626:Sample 9

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mixed

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

text: Sample 9


E-MTAB-11626:Sample 10

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11626:Sample 10

text: ArrayExpress

text: mixed

text: human

text: adult

text: normal

text: wild type genotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 10


E-MTAB-11626:Sample 1

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11626:Sample 1

characteristic: Submitter Id

text: ArrayExpress

text: mixed

text: human

text: adult

text: normal

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: plate

text: Homo sapiens

text: male

characteristic: sex

text: Sample 1


E-MTAB-11626:Sample 2

text: public

text: E-MTAB-11626:Sample 2

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mixed

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

text: Sample 2

characteristic: title


E-MTAB-11625:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11625:Sample 1

text: ArrayExpress

text: memory B cell

text: human

text: Protocols: All spleen in this study were collected from organ donors who died from stroke or head trauma. None of them had lymphoma or autoimmune disease. Immediately after splenectomy, mononuclear cells were isolated from crushed pieces of splenic tissue by ficoll density-gradient centrifugation and stored in liquid nitrogen. After thawing, mononuclear cells were counted and stained with antibodies directed against surface markers. CD21hiCD20hi (CD21high IgG+ memory B cell) and CD21intCD20int memory B cell subsets (CD21int IgG+ memory B cell) were then bulk FACS-sorted (AriaIII) from gated IgG+ switched memory B cells (IgG+CD27+CD20+IgD-) in parallel with naives B cells (CD20+CD38lowCD24intCD27-IgD+CD3-CD14-CD16+). Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) was performed on 50000 cells of each sorted population according to published methods (Buenrostro, Nature Methods, 2013, PMID: 24097267). Libraries were amplified and prepared according to published methods (Buenrostro, Nature Methods, 2013, PMID: 24097267).

text: adult

text: normal

text: CD21high IgG+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

text: Sample 1


E-MTAB-11625:Sample 2

characteristic: ENA-CHECKLIST

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11625:Sample 2

characteristic: Submitter Id

text: ArrayExpress

text: memory B cell

text: human

text: adult

text: normal

text: CD21int IgG+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 2


E-MTAB-11625:Sample 3

text: public

text: E-MTAB-11625:Sample 3

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

characteristic: sex

text: Sample 3


E-MTAB-11625:Sample 4

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-11625:Sample 4

text: ArrayExpress

text: memory B cell

text: human

text: adult

text: normal

characteristic: disease

text: CD21high IgG+

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

text: Sample 4

characteristic: title


E-MTAB-11625:Sample 5

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11625:Sample 5

text: ArrayExpress

text: memory B cell

text: human

text: adult

text: normal

text: CD21int IgG+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: spleen

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-11625:Sample 6

text: public

characteristic: SRA accession

text: E-MTAB-11625:Sample 6

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

text: human

text: adult

text: normal

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: spleen

characteristic: organism part

text: Homo sapiens

text: female

text: Sample 6


E-MTAB-11625:Sample 7

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-11625:Sample 7

text: ArrayExpress

text: memory B cell

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: normal

text: CD21high IgG+

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

characteristic: sex

text: Sample 7


E-MTAB-11625:Sample 8

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11625:Sample 8

text: ArrayExpress

text: memory B cell

text: human

text: adult

text: normal

characteristic: disease

text: CD21int IgG+

text: Homo sapiens

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

text: Sample 8

characteristic: title


E-MTAB-11625:Sample 9

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11625:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: naive B cell

text: human

text: adult

text: normal

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: spleen

text: Homo sapiens

text: male

text: Sample 9


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE DE LIMOGES

text: public

tag: Namespace:UNIVERSITE DE LIMOGES

text: house mouse

text: Mutation analysis within JH4 intron in WT 1

text: Mus musculus

text: Mus musculus


characteristic: ENA-LAST-UPDATE

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

characteristic: common name

text: Mutation analysis within JH4 intron in WT 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 4

characteristic: description

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 8

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 9

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 8

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 8

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 9

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 10

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 8

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 6

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 7

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 8

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 9

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in WT 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JH4 intron in SATB1cKO 8 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in WT 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within JK5 intron in SATB1cKO 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in WT 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 1 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 2 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 3 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 4 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 5 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 6 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mutation analysis within Smu region in SATB1cKO 7 NPCGG IMMUNIZED

text: Mus musculus

text: Mus musculus


text: public

tag: Namespace:Interfactulty Bioinformatics Unit, Department of Biology, University of Bern

text: ArrayExpress

characteristic: broker name

text: stromal cell

text: house mouse

text: Protocols: FACS Next GEM Single Cell 3' Reagent Kit v3.1 Next GEM Single Cell 3' Reagent Kit v3.1

text: adult

characteristic: developmental stage

text: normal

text: wild type genotype

characteristic: genotype

text: CD45 negative

characteristic: immunophenotype

text: mixed

characteristic: individual

text: Mus musculus

text: Mus musculus

text: mandibular lymph node

characteristic: organism part

text: Mus musculus

text: mixed

characteristic: strain

text: mandLN


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Interfactulty Bioinformatics Unit, Department of Biology, University of Bern

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: stromal cell

text: house mouse

text: adult

text: normal

characteristic: disease

text: wild type genotype

text: CD45 negative

text: mixed

text: Mus musculus

text: Mus musculus

text: mesenteric lymph node

text: Mus musculus

text: mixed

characteristic: sex

text: MLN

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: stromal cell

text: house mouse

text: adult

text: normal

text: wild type genotype

text: CD45 negative

text: mixed

text: Mus musculus

characteristic: organism

text: Mus musculus

text: peripheral lymph node

text: Mus musculus

text: mixed

text: PLN


text: public

tag: Namespace:UNIVERSITY OF COPENHAGEN, SECTION OF MICROBIOLOGY

characteristic: description

text: gut metagenome

text: gut metagenome


text: UNIVERSITY OF COPENHAGEN, SECTION OF MICROBIOLOGY

text: public

text: gut metagenome

text: gut metagenome


text: public

text: gut metagenome

text: gut metagenome


text: public

text: Pro-sham+FVT

text: gut metagenome

text: gut metagenome


text: public

text: LGG+Saline

text: gut metagenome

text: gut metagenome


text: public

text: Pro-sham+Saline

text: gut metagenome

text: gut metagenome


text: public

text: LGG+FVT

text: gut metagenome

text: gut metagenome


text: Universite du Quebec a Montreal

text: public

tag: Namespace:Universite du Quebec a Montreal

characteristic: collection date

text: Porewater

text: coastal

characteristic: environment (biome)

text: aquifer

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: uncultured prokaryote

text: Mataro

characteristic: project name

text: uncultured prokaryote

text: Illumina MiSeq

characteristic: sequencing method

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite du Quebec a Montreal

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Groundwater

text: coastal

text: aquifer

text: water

text: Spain

text: metagenome

text: uncultured prokaryote

text: Mataro

text: uncultured prokaryote

text: Illumina MiSeq

characteristic: title

text: water


text: Universite du Quebec a Montreal

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Seawater

characteristic: description

text: coastal

characteristic: environment (biome)

text: sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Spain

characteristic: geographic location (country and/or sea)

text: metagenome

text: uncultured prokaryote

characteristic: organism

text: Mataro

text: uncultured prokaryote

text: Illumina MiSeq

text: Sea

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Institute of Infection, Immunity and Inflammation, University of Glasgow

text: ArrayExpress

characteristic: broker name

text: malaria parasite P. falciparum

characteristic: common name

text: Protocols: P. falciparum cell culture was performed as described previously (Brancucci, 2015; Trager, 1976). RPMI-1640 culture media were supplemented with 25 mM HEPES, 100 μM hypoxanthine, 24 mM sodium bicarbonate, and gentamicin (all from Sigma-Aldrich). Media containing serum were generated by additional supplementation of 10% O+ human serum (The Interstate Companies). Minimal fatty acid (mFA) media were generated by additional supplementation of 0.39% fatty acid-free BSA, 30 μM oleic acid, and 30 μM palmitic acid (all from Sigma-Aldrich). To generate serum-free media supplemented with 20 μM LysoPC, LysoPC (Avanti Polar Lipids) was dissolved in ethanol and dried on culture dishes prior to culture addition. All cell cultures were maintained in a 5% CO2, 1% O2 and 94 % N2 gas mixture (Med-Tech Gases). Experiments used a clone of the Pf2004/164TdTomato P. falciparum line17, which requires addition of 4 nM WR99210 (Jacobus Pharmaceuticals) to select for stable maintenance of episomes. Additional experiments were performed with strain Dd2, which is a clone derived from W2-MEF which itself is derived from W2’82 (Indochina III/CDC) (Oduola, 1988; Wellems, 1990). Per time point 1mL PCV at ~3% parasitaemia were treated has fallow. Parasites were isolate by saponin treatment (0.01%). RNA extraction was performed with RNeasy plus kit from Qiagen, parasite pellets were resuspended in 350 ¬µl of lysis buffer and stored at -80 degrees C utile extraction. Up to 10 µg of RNA was treated with DNAse Turbo kit (Ambion) prior reverse transcription or RNA seq. RNA quality was check with the Agilent bioanalyzer. 150 ng total RNA of each sample was used for library preparation. RNA quality was check with the Agilent bioanalyzer. Stranded libraries were prepared by using TruSeq Stranded mRNA kit (Illumina) using manufacturer instructions with 13 cycles of PCR. Library quantification was conducted by Qubit measurements using the HS DNA Kit.

text: IDC

characteristic: developmental stage

text: Ben knockout

characteristic: genotype

text: serum

characteristic: growth condition

text: Plasmodium falciparum

text: Plasmodium falciparum

text: whole organism

characteristic: organism part

text: Plasmodium falciparum

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Infection, Immunity and Inflammation, University of Glasgow

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: malaria parasite P. falciparum

text: IDC

text: Ben knockout

text: serum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: whole organism

text: Plasmodium falciparum

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: malaria parasite P. falciparum

characteristic: description

text: IDC

text: Ben knockout

text: minimum Fatty Acid medium

text: Plasmodium falciparum

characteristic: organism

text: Plasmodium falciparum

text: whole organism

text: Plasmodium falciparum


text: public

text: ArrayExpress

characteristic: broker name

text: malaria parasite P. falciparum

characteristic: common name

text: IDC

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: serum

text: Plasmodium falciparum

text: Plasmodium falciparum

text: whole organism

text: Plasmodium falciparum


characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: malaria parasite P. falciparum

text: IDC

text: wild type genotype

text: minimum Fatty Acid medium

characteristic: growth condition

text: Plasmodium falciparum

text: Plasmodium falciparum

text: whole organism

characteristic: organism part

text: Plasmodium falciparum

characteristic: strain


characteristic: External Id

text: UNIVERSITY OF VIENNA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:UNIVERSITY OF VIENNA

text: human

text: Human sample from Verteba cave

text: Homo sapiens

text: Homo sapiens


text: UNIVERSITY OF SASSARI

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF SASSARI

characteristic: Submitter Id

text: gut metagenome

text: gut metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: MAX PLANCK INSTITUE OF CHEMCIAL ECOLOGY

text: public

tag: Namespace:MAX PLANCK INSTITUE OF CHEMCIAL ECOLOGY

text: large cabbage white

text: Genome sequences

characteristic: description

text: Pieris brassicae

characteristic: organism

text: Pieris brassicae

text: Pieris brassicae


text: MPIPZ

text: public

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

text: Protocols: Leaves were removed from plants and stored at -80 C until DNA extraction Greenhouse supplemented with additional light. Plants grown in the greenhouse (16 h day/8 h night) for 3 weeks and 4 days in the dark NucleoMag Plant Kit (Macherey and Nagel, P/O 744400.4) on a robotic device (KingFisher, Thermo) TPase-based DNA library (>95 samples)

text: sporophyte vegetative stage

characteristic: developmental stage

text: Col x Ler

characteristic: ecotype

text: zyp1-1/zyp1-6 mutant

characteristic: genotype

text: green house study

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana

text: female

characteristic: sex


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIPZ

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: thale cress

text: sporophyte vegetative stage

text: Col x Ler

text: zyp1-1/zyp1-6 mutant

text: green house study

characteristic: growth condition

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

characteristic: organism part

text: Arabidopsis thaliana

text: male


characteristic: ENA-CHECKLIST

text: MPIPZ

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: thale cress

text: sporophyte vegetative stage

text: Col x Ler

text: wild type genotype

text: green house study

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: MPIPZ

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: thale cress

characteristic: common name

characteristic: description

text: sporophyte vegetative stage

text: Col x Ler

text: wild type genotype

text: green house study

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana

text: male


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPIPZ

text: public

text: ArrayExpress

text: thale cress

text: sporophyte vegetative stage

characteristic: developmental stage

text: Col x Ler

characteristic: ecotype

text: zyp1-1/zyp1-6 HEI10oe heterozygous mutant

characteristic: genotype

text: green house study

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

text: MPIPZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: thale cress

text: sporophyte vegetative stage

text: Col x Ler

text: green house study

characteristic: growth condition

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

characteristic: organism part

text: Arabidopsis thaliana

text: male


characteristic: ENA-FIRST-PUBLIC

text: MPIPZ

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: thale cress

text: sporophyte vegetative stage

text: Col x Ler

text: HEI10oe heterozygous mutant

text: green house study

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana

text: female

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUE OF CHEMCIAL ECOLOGY

text: public

text: large cabbage white

characteristic: common name

text: Genome assembly

characteristic: description

text: Pieris brassicae

characteristic: organism

text: Pieris brassicae


characteristic: ENA-CHECKLIST

text: The Pirbright Institute

text: public

tag: Namespace:The Pirbright Institute

text: African malaria mosquito

text: transcriptome of 3rd instar larvae from transgenic line 23 with ectopic expression of Yob gene

text: 3rd instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: female

characteristic: sex


text: The Pirbright Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: African malaria mosquito

text: 3rd instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: male


text: The Pirbright Institute

characteristic: INSDC last update

text: public

text: African malaria mosquito

text: transcriptome of a 24h-old pupa from transgenic line 23 with ectopic expression of Yob gene

text: pupa

text: Anopheles gambiae

text: Anopheles gambiae

text: female


text: The Pirbright Institute

text: public

text: African malaria mosquito

text: transcriptome of 3rd instar larvae: progeny of males with sgRNA targeting Yob gene and females expressing Cas9 in the germline

text: 3rd instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: male


text: The Pirbright Institute

text: public

text: African malaria mosquito

text: 3th instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: male


text: The Pirbright Institute

text: public

text: African malaria mosquito

text: transcriptome of 24h-old pupae: progeny of males with sgRNA targeting Yob gene and females expressing Cas9 in the germline

text: pupae

text: Anopheles gambiae

text: Anopheles gambiae

text: male


text: The Pirbright Institute

text: public

text: African malaria mosquito

text: transcriptome of wild-type G3 strain 3rd instar larvae

text: 3th instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: female


text: The Pirbright Institute

text: public

text: African malaria mosquito

text: 3th instar larvae

text: Anopheles gambiae

text: Anopheles gambiae

text: male


23_P_F1 _part1

text: The Pirbright Institute

text: public

text: 23_P_F1 _part1

text: African malaria mosquito

text: pupa

text: Anopheles gambiae

text: Anopheles gambiae

text: female

text: 23_P_F1 _part1


text: UZH

text: public

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: The BY4741 (MATa ura3 his3 leu2 met15 TRP1) was used as a parental strain in which the promoter of Npl3 was replaced with an inducible gal promoter by homologues recombination. The strain was complemented with pRS315::Npl3500Kb::HRV-3C::CterHTP plasmid. In this plasmid, the expression of Npl3 is driven by its endogenous promoter. A HRV-C3 protease cleavage site was inserted between amino acids S282 and N283 using PCR. A His-Trypsin-Protein A (HTP) tandem tag was placed at the C-terminus of the protein by PCR. The split-iCRAC was based on the original CRAC protocol described by Granneman et al. 2009 with some modifications (Granneman, Kudla et al., 2009). Briefly, the recombinant yeast strains were grown in SD-leu medium to drive Npl3 expression only from the transformed plasmid. 2 L of yeast culture were harvested at an OD600 of ~2. The cells were resuspended in 1 v/w SD-Trp medium and UV-irradiated (1.6 J/cm2) in Petri dishes in a Stratalinker 1600 (Stratagene). Half the cells were not subjected to UV treatment and were kept as the UV minus control. Cells were pelleted and resuspended in 25 ml of lysis buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 0.15% NP-40) with addition of 1.3 mM PMSF, 1 mM DDT, complete protease inhibitor cocktail (Roche)). The cells were lysed using 25 ml glass beads in Planetary mill for 20 mins at 500 rpm. 5 ml of lysis buffer was added, and the lysate was cleared by centrifugation (20 min at 5000 RPM and 4 °C followed by 2x 20 min at 18000 RPM and 4 °C). Cleared lysate was incubated with 300 of IgG bead suspension (1:1) for 2 h at 4 °C with rotation. Beads were collected and washed 3x with 10 ml wash buffer (50 mM Tris-HCl pH 7.8, 1 M NaCl, 0.15% NP-40, 0.5 mM DDT) followed by 3x with 10 ml lysis buffer with added 0.5 mM DDT. Beads were resuspended in 5 ml of lysis buffer and rotated with 40 g of homemade TEV for 18 h at 4 °C. The eluates were concentrated to a volume of 500 l in 30 kDa cutoff centricons (Millipore). No RNase treatment was performed in the final experiments for library preparation. 0.4 g of Guanidine-HCl were dissolved in the eluate to yield a final concentration of 6M. NaCl and imidazole were added to a final concentration of 300 and 10 mM, respectively. 100 l of pre-equilibrated Ni-beads were added to the samples and incubated for 2h at 4 °C. Beads were washed 3x with wash buffer (6 M guanidine-HCl, 50 mM Tris-HCl (pH 7.8), 300 mM NaCl, 0.1% NP-40, 40 mM imidazole, and 1 mM DDT) followed by 3x with PNK buffer (50 mM Tris-HCl (pH 7.8), 40 mM MgCl2, 0.1% NP-40, and 1 mM DDT). The beads were then incubated in 50 l of PNK buffer (pH 6.5) in the presence of 10 units of PNK (NEB) and 10 units of CIP (NEB) and 20 units of SUPERase.In (Ambion) for 30 mins at 37 °C. Beads were washed 3x with wash buffer followed by 2x with PNK buffer (pH 7.8) and incubated with 50 pmoles of L3 adapter (rAppAGATCGGAAGAGCGGRRCAG/ddC/) in 50 l of ligation mixture (1x PNK buffer (pH 7.8) supplemented with 12.5 units of T4 RNA ligase 1 (NEB), 250 units of T4 RNA ligase 2 truncated K227Q (NEB), 40 units of SUPERase.In (Ambion) and 10% PEG 4000). The reaction is incubated for 18 h with mild shaking at 16 °C. Beads are then washed with 3x wash buffer followed by 3x PNK buffer. For the PreScission cleaved version, the beads are then incubated with 1x PNK buffer (pH 7.8) supplemented with 20 units of SUPERase.In (Ambion) and 10 units of PreScission protease (GE Healthcare) for 18 h at 4 °C with mild shaking. The mixture is then supplemented with 10 units of PNK and 0.5 l of 32P-ATP and incubated for 1 h at 37 °C. 20 l of 4x LDS is added to the beads, boiled for 10 mins and resolved on a 4-12% Bis-Tris NuPage gel (Invitrogen). Subsequent steps of RNA isolation and library preparation were done as described in Huppertz et al. 2014 Methods (Huppertz et al., 2014). The negative control sample was generated following the same procedure but starting with WT yeast strain that does not express any tagged protein.

characteristic: genotype

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: whole organism

characteristic: organism part

text: Saccharomyces cerevisiae

characteristic: strain


characteristic: ENA-FIRST-PUBLIC

text: UZH

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: whole organism

text: Saccharomyces cerevisiae

text: negative


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in WT 1

text: Mus musculus

text: Mus musculus

characteristic: title


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

characteristic: common name

text: Repertoire analysis in WT 2

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus


characteristic: ENA-CHECKLIST

text: UNIVERSITE DE LIMOGES

characteristic: INSDC center name

text: public

text: house mouse

text: Repertoire analysis in WT 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Repertoire analysis in WT 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in WT 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 1

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 2

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 3

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 4

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 5

text: Mus musculus

text: Mus musculus


text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Repertoire analysis in SATB1cKO 6

text: Mus musculus

text: Mus musculus


text: public

tag: Namespace:Scottish Microbiology Reference Laboratories

characteristic: building ID

characteristic: building type

characteristic: collection date

characteristic: compartment ID

unit: CFU/L

characteristic: culturable Legionella

text: premise plumbing water

characteristic: environment (biome)

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Warsaw

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: PowerWater DNA isolation kit (Qiagen)

characteristic: nucleic acid extraction

text: drinking water metagenome

characteristic: pH

text: V3V4-F:TACGGGAGGCAGCAG, V3V4-R:CCAGGGTATCTAATCC

characteristic: pcr primers

text: municipal water

characteristic: plumbing component

text: Bacterial communities and culturable Legionella in premise plumbing of four European cities

characteristic: project name

unit: mL

characteristic: sample volume or weight for DNA extraction

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

unit: ºC

characteristic: temperature

text: Premise plumbing water, Warsaw building 1, municipal water, winter 2017

text: water

characteristic: water environmental package


tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Scottish Microbiology Reference Laboratories

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: building ID

characteristic: building type

characteristic: collection date

characteristic: compartment ID

characteristic: culturable Legionella

text: premise plumbing water

characteristic: description

characteristic: environment (biome)

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

text: Warsaw

text: mimarks-survey

text: PowerWater DNA isolation kit (Qiagen)

text: drinking water metagenome

characteristic: organism

text: hot recirculating water

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

text: 16S rRNA

text: Premise plumbing water, Warsaw building 1, hot recirc water, winter 2017

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: premise plumbing water

text: Poland

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Warsaw

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: PowerWater DNA isolation kit (Qiagen)

characteristic: nucleic acid extraction

text: drinking water metagenome

characteristic: pH

characteristic: pcr primers

text: farthest point from boiler

characteristic: plumbing component

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: Premise plumbing water, Warsaw building 1, farthest point from boiler, winter 2017

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: building ID

text: Hotel

characteristic: building type

characteristic: collection date

characteristic: compartment ID

characteristic: culturable Legionella

text: premise plumbing water

characteristic: description

characteristic: environment (biome)

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

text: Warsaw

text: mimarks-survey

text: PowerWater DNA isolation kit (Qiagen)

text: drinking water metagenome

characteristic: organism

text: municipal water

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

text: 16S rRNA

text: Premise plumbing water, Warsaw building 2, municipal water, winter 2017

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Hotel

text: premise plumbing water

text: Poland

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Warsaw

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: PowerWater DNA isolation kit (Qiagen)

characteristic: nucleic acid extraction

text: drinking water metagenome

characteristic: pH

characteristic: pcr primers

text: bottom of boiler

characteristic: plumbing component

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: Premise plumbing water, Warsaw building 2, bottom of boiler, winter 2017

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: building ID

text: Hotel

characteristic: building type

characteristic: collection date

characteristic: compartment ID

characteristic: culturable Legionella

text: premise plumbing water

characteristic: description

characteristic: environment (biome)

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

text: Warsaw

text: mimarks-survey

text: PowerWater DNA isolation kit (Qiagen)

text: drinking water metagenome

characteristic: organism

text: hot recirculating water

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

text: 16S rRNA

text: Premise plumbing water, Warsaw building 2, hot recirc water, winter 2017

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Hotel

text: premise plumbing water

text: Poland

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Warsaw

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: PowerWater DNA isolation kit (Qiagen)

characteristic: nucleic acid extraction

text: drinking water metagenome

characteristic: pH

characteristic: pcr primers

text: farthest point from boiler

characteristic: plumbing component

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: Premise plumbing water, Warsaw building 2, farthest point from boiler, winter 2017

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: building ID

text: Hospital

characteristic: building type

characteristic: collection date

characteristic: compartment ID

characteristic: culturable Legionella

text: premise plumbing water

characteristic: description

characteristic: environment (biome)

characteristic: environment (material)

text: Poland

characteristic: geographic location (country and/or sea)

text: Warsaw

text: mimarks-survey

text: PowerWater DNA isolation kit (Qiagen)

text: drinking water metagenome

characteristic: organism

text: hot recirculating water

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

text: 16S rRNA

text: Premise plumbing water, Warsaw building 3, hot recirc water, winter 2017

characteristic: title

text: water


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: premise plumbing water

text: Poland

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Warsaw

characteristic: geographic location (region and locality)

text: mimarks-survey

characteristic: investigation type

text: PowerWater DNA isolation kit (Qiagen)

characteristic: nucleic acid extraction

text: drinking water metagenome

characteristic: pH

characteristic: pcr primers

text: municipal water

characteristic: plumbing component

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: drinking water metagenome

text: Illumina MiSeq v3 PE300

characteristic: sequencing method

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: Premise plumbing water, Warsaw building 1, municipal water, summer 2017

text: water

characteristic: water environmental package