text: ETH Zurich

text: public

tag: Namespace:ETH Zurich

characteristic: collection date

text: greenhouse mesocosm experiment - Severe

text: forest

characteristic: environment (biome)

text: pine forest

characteristic: environment (feature)

text: soil environment

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: TSMWD

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: greenhouse mesocosm experiment - Control

characteristic: description

text: forest

text: pine forest

text: soil environment

text: Switzerland

unit: m

unit: DD

text: metagenome

text: soil metagenome

characteristic: organism

text: TSMWD

text: soil metagenome

text: Illumina

text: soil

characteristic: title


text: ETH Zurich

text: public

characteristic: collection date

text: greenhouse mesocosm experiment - Intermediate

text: forest

characteristic: environment (biome)

text: pine forest

characteristic: environment (feature)

text: soil environment

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: TSMWD

text: soil metagenome

text: Illumina

text: soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITE DE LIMOGES

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:UNIVERSITE DE LIMOGES

text: house mouse

characteristic: common name

text: Mutation analysis within Smu region in UNGMAR KO 5

text: Mus musculus

text: Mus musculus


text: Harvard Medical School

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Harvard Medical School

characteristic: Submitter Id

text: human

characteristic: description

text: Israel

text: Southwest Israel, Tell el-Hesi

characteristic: geographic location (region and locality)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: F

characteristic: sex

characteristic: title


text: THE UNIVERSITY OF MELBOURNE

text: public

tag: Namespace:THE UNIVERSITY OF MELBOURNE

text: human

text: Long read dRNA sequencing of SH-SY5Y cell line

text: Homo sapiens

text: Homo sapiens


E-MTAB-11782:CL1 siFoxa2 1

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL1 siFoxa2 1

tag: Namespace:MRC Cancer Unit University of Cambridge

text: ArrayExpress

characteristic: broker name

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: Protocols: Cells lysed in RLT buffer RNA extracted using RNeasy plus kit (Qiagen, #74034) Library constructed using TruSeq Stranded mRNA kit (Illumina, #20020594)

text: adult

characteristic: developmental stage

text: hereditary leiomyomatosis and renal cell cancer

characteristic: disease

text: Fh1 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: CL1 siFoxa2 1


E-MTAB-11782:CL1 siFoxa2 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11782:CL1 siFoxa2 2

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL1 siFoxa2 2


E-MTAB-11782:CL1 siFoxa2 3

text: MRC Cancer Unit University of Cambridge

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11782:CL1 siFoxa2 3

characteristic: Submitter Id

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL1 siFoxa2 3


E-MTAB-11782:CL19 siFoxa2 1

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL19 siFoxa2 1

text: ArrayExpress

characteristic: broker name

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

text: adult

characteristic: developmental stage

text: Fh1 knockout

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: CL19 siFoxa2 1

characteristic: title


E-MTAB-11782:CL19 siFoxa2 2

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL19 siFoxa2 2

text: ArrayExpress

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

text: adult

characteristic: disease

text: Fh1 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: CL19 siFoxa2 2


E-MTAB-11782:CL19 siFoxa2 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL19 siFoxa2 3

text: ArrayExpress

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL19 siFoxa2 3


E-MTAB-11782:FLFL siFoxa2 1

text: MRC Cancer Unit University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11782:FLFL siFoxa2 1

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siFoxa2 1


E-MTAB-11782:FLFL siFoxa2 2

text: MRC Cancer Unit University of Cambridge

text: public

characteristic: SRA accession

text: E-MTAB-11782:FLFL siFoxa2 2

characteristic: Submitter Id

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siFoxa2 2


E-MTAB-11782:FLFL siFoxa2 3

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:FLFL siFoxa2 3

text: ArrayExpress

characteristic: broker name

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

characteristic: developmental stage

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siFoxa2 3

characteristic: title


E-MTAB-11782:CL1 siNT 1

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL1 siNT 1

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: adult

characteristic: disease

text: Fh1 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: CL1 siNT 1


E-MTAB-11782:CL1 siNT 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL1 siNT 2

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL1 siNT 2


E-MTAB-11782:CL1 siNT 3

text: MRC Cancer Unit University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11782:CL1 siNT 3

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL1 siNT 3


E-MTAB-11782:CL19 siNT 1

text: MRC Cancer Unit University of Cambridge

text: public

characteristic: SRA accession

text: E-MTAB-11782:CL19 siNT 1

characteristic: Submitter Id

text: ArrayExpress

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: CL19 siNT 1


E-MTAB-11782:CL19 siNT 2

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL19 siNT 2

text: ArrayExpress

characteristic: broker name

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

text: adult

characteristic: developmental stage

text: Fh1 knockout

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: CL19 siNT 2

characteristic: title


E-MTAB-11782:CL19 siNT 3

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:CL19 siNT 3

text: ArrayExpress

text: Fh1 -/- CL19

text: epithelial cell

text: house mouse

text: adult

characteristic: disease

text: Fh1 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: CL19 siNT 3


E-MTAB-11782:FLFL siNT 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit University of Cambridge

text: public

text: E-MTAB-11782:FLFL siNT 1

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siNT 1


E-MTAB-11782:FLFL siNT 2

text: MRC Cancer Unit University of Cambridge

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11782:FLFL siNT 2

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siNT 2


E-MTAB-11782:FLFL siNT 3

text: MRC Cancer Unit University of Cambridge

text: public

characteristic: SRA accession

text: E-MTAB-11782:FLFL siNT 3

characteristic: Submitter Id

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: FLFL siNT 3


text: public

tag: Namespace:University of Oxford, Sir William Dunn School of Pathology

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 01 from patient B2.

text: Homo sapiens

characteristic: organism

characteristic: patient age at collection of tumor

text: Male

characteristic: patient sex

characteristic: patient tumor diagnosis at time of collection

characteristic: patient tumor primary site

characteristic: patient tumor site of collection

text: Metastatic Neoplasm

characteristic: patient tumor type

text: tissue fragment

characteristic: sample material

text: Patient tumor

characteristic: sample origin

text: Homo sapiens

characteristic: sample taxon name

characteristic: sample unique ID

text: Homo sapiens

text: Patient B2 pre-treatment R1 reads for sample 01

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Oxford, Sir William Dunn School of Pathology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 02 from patient B2.

text: Homo sapiens

text: Male

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: MM503_02_replacement_S125_R1_001.fastq.gz

text: Homo sapiens

text: Patient B2 pre-treatment R1 reads for sample 02


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 03 from patient B2.

characteristic: description

text: Homo sapiens

characteristic: organism

characteristic: patient age at collection of tumor

text: Male

characteristic: patient sex

characteristic: patient tumor diagnosis at time of collection

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: MM503_03_replacement_S126_R1_001.fastq.gz

text: Homo sapiens

text: Patient B2 pre-treatment R1 reads for sample 03


text: public

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from post-treatment sample PT1 from patient B2

text: Homo sapiens

text: Male

characteristic: patient tumor primary site

characteristic: patient tumor site of collection

text: Metastatic Neoplasm

characteristic: patient tumor type

text: tissue fragment

characteristic: sample material

text: Patient tumor

characteristic: sample origin

text: Homo sapiens

characteristic: sample taxon name

characteristic: sample unique ID

text: Homo sapiens

text: Patient B2 post-treatment R1 reads for sample PT1

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from post-treatment sample PT2 from patient B2

text: Homo sapiens

text: Male

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Patient B2 post-treatment R1 reads for sample PT2


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample from patient C1

characteristic: description

text: Homo sapiens

characteristic: organism

characteristic: patient age at collection of tumor

text: Male

characteristic: patient sex

text: Osteosarcom

characteristic: patient tumor diagnosis at time of collection

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Patient C1 pre-treatment R1 reads


text: public

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 001 from patient C2

text: Homo sapiens

text: Male

characteristic: patient tumor primary site

characteristic: patient tumor site of collection

text: Metastatic Neoplasm

characteristic: patient tumor type

text: tissue fragment

characteristic: sample material

text: Patient tumor

characteristic: sample origin

text: Homo sapiens

characteristic: sample taxon name

characteristic: sample unique ID

text: Homo sapiens

text: Patient C2 pre-treatment R1 reads for sample 001

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 002 from patient C2

text: Homo sapiens

text: Male

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Patient C2 pre-treatment R1 reads for sample 002


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample 003 from patient C2

characteristic: description

text: Homo sapiens

characteristic: organism

characteristic: patient age at collection of tumor

text: Male

characteristic: patient sex

characteristic: patient tumor diagnosis at time of collection

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Patient C2 pre-treatment R1 reads for sample 003


text: University of Oxford, Sir William Dunn School of Pathology

text: public

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample Tr01 from patient C3

text: Homo sapiens

text: Female

characteristic: patient tumor primary site

text: Soft Tissue|NCIT:C12471|http://purl.obolibrary.org/obo/NCIT_C12471

characteristic: patient tumor site of collection

text: Metastatic Neoplasm

characteristic: patient tumor type

text: tissue fragment

characteristic: sample material

text: Patient tumor

characteristic: sample origin

text: Homo sapiens

characteristic: sample taxon name

characteristic: sample unique ID

text: Homo sapiens

text: Patient C3 pre-treatment R1 reads for sample Tr01

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Fastq.gz file containing R1 reads from paired-end sequencing of RNA from pre-treatment sample Tr02 from patient C3

text: Homo sapiens

text: Female

text: Metastatic Neoplasm

text: tissue fragment

text: Patient tumor

text: Homo sapiens

text: Homo sapiens

text: Patient C3 pre-treatment R1 reads for sample Tr02


text: University of Manchester

text: public

characteristic: SRA accession

tag: Namespace:University of Manchester

characteristic: Submitter Id

text: laboratory facility

characteristic: broad-scale environmental context

characteristic: collection date

text: 16S rRNA sequencing of V3 and V4 region, mouse faeces

characteristic: description

text: feces

characteristic: environmental medium

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

characteristic: geographic location (longitude)

unit: week

characteristic: host age

text: mouse

characteristic: host common name

text: WT

characteristic: host genotype

text: female

characteristic: host sex

characteristic: host subject id

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: animal cage

characteristic: local environmental context

text: mouse metagenome

characteristic: organism

text: 16S Amplicon PCR Forward Primer (5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG) and 16S Amplicon PCR Reverse Primer (5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC)

characteristic: pcr primers

text: Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism

characteristic: project name

text: mouse metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: timepoint


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Manchester

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: laboratory facility

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: description

text: feces

characteristic: environmental medium

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

text: mouse

text: IgMi KO

text: female

text: host-associated

text: metagenome

text: animal cage

text: mouse metagenome

text: mouse metagenome

text: Sequencing by synthesis (Illumina)

text: 16s rRNA

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Manchester

text: public

text: laboratory facility

text: feces

text: United Kingdom

text: not collected

text: not collected

characteristic: geographic location (longitude)

characteristic: host age

text: mouse

characteristic: host common name

text: HET

characteristic: host genotype

text: female

characteristic: host sex

characteristic: host subject id

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: animal cage

characteristic: local environmental context

text: mouse metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: mouse metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: timepoint


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Manchester

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: laboratory facility

characteristic: broad-scale environmental context

text: not collected

characteristic: collection date

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: geographic location (latitude)

text: not collected

text: mouse

text: host-associated

text: metagenome

text: not collected

text: mouse metagenome

text: mouse metagenome

text: Sequencing by synthesis (Illumina)

text: 16s rRNA

text: water control

characteristic: title


text: University of Manchester

text: public

text: laboratory facility

text: not collected

text: United Kingdom

text: not collected

text: not collected

characteristic: geographic location (longitude)

characteristic: host age

text: mouse

characteristic: host common name

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: not collected

characteristic: local environmental context

text: mouse metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: mouse metagenome

text: Sequencing by synthesis (Illumina)

characteristic: sequencing method

text: 16s rRNA

characteristic: target gene

characteristic: target subfragment

text: Kitome control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Orebro University Hospital

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Orebro University Hospital

characteristic: Submitter Id

text: not provided, US, NA

characteristic: country

characteristic: genotype

text: Homo sapiens

characteristic: host

text: Yes

text: Mutant

text: Neisseria gonorrhoeae

text: Mutant

characteristic: pathotype

text: Neisseria gonorrhoeae

text: Mutant

characteristic: serovar

characteristic: strain


E-MTAB-11785:Fh1 -/- CL1 FOXA2 rep 1

text: MRC Cancer Unit

text: public

text: E-MTAB-11785:Fh1 -/- CL1 FOXA2 rep 1

tag: Namespace:MRC Cancer Unit

text: ArrayExpress

characteristic: broker name

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

characteristic: common name

text: Protocols: Cells were fixed with 1% HCHO for 10 minutes and scraped into ice-cold PBS. Cells were lysed with ChIP lysis buffers and chromatin was sonicated for 15 minutes. Chromatin was incubated with Dynabeads with either anti-Foxa2 or IgG antibody overnight at 4deg. Beads were washed and incubated with Tn5 buffer and Tn5 enzyme for 10 minutes at 37deg with vigorous shaking. DNA was then eluted from the beads and purified. Purified DNA was amplified using Nextera indexes.

characteristic: description

text: adult

characteristic: developmental stage

text: hereditary leiomyomatosis and renal cell cancer

characteristic: disease

text: Fh1 knockout

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: Fh1 -/- CL1 FOXA2 rep 1

characteristic: title


E-MTAB-11785:Fh1 -/- CL1 FOXA2 rep 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11785:Fh1 -/- CL1 FOXA2 rep 2

text: ArrayExpress

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

text: adult

text: Fh1 knockout

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: Fh1 -/- CL1 FOXA2 rep 2


E-MTAB-11785:Fh1 -/- CL1 IgG

text: MRC Cancer Unit

text: public

text: E-MTAB-11785:Fh1 -/- CL1 IgG

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Fh1 -/- CL1

text: epithelial cell

text: house mouse

characteristic: common name

text: adult

text: Fh1 knockout

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: Fh1 -/- CL1 IgG


E-MTAB-11785:Fh1 fl/fl FOXA2 rep 1

text: MRC Cancer Unit

text: public

text: E-MTAB-11785:Fh1 fl/fl FOXA2 rep 1

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

characteristic: description

text: adult

characteristic: developmental stage

characteristic: disease

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: kidney

text: Mus musculus

text: Fh1 fl/fl FOXA2 rep 1


E-MTAB-11785:Fh1 fl/fl FOXA2 rep2

characteristic: ENA-CHECKLIST

text: MRC Cancer Unit

text: public

text: E-MTAB-11785:Fh1 fl/fl FOXA2 rep2

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

characteristic: organism part

characteristic: replicate

text: Mus musculus

text: Fh1 fl/fl FOXA2 rep2

characteristic: title


E-MTAB-11785:Fh1 fl/fl IgG

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC Cancer Unit

characteristic: INSDC center name

text: public

text: E-MTAB-11785:Fh1 fl/fl IgG

text: ArrayExpress

text: Fh1 fl/fl

text: epithelial cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: kidney

text: Mus musculus

text: Fh1 fl/fl IgG


text: GENGS-CBMSO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: GENGS-CBMSO

text: Unpaired U1 reads

text: Thermus thermophilus HB27

text: Thermus thermophilus HB27

text: Unpaired U1 reads


text: GENGS-CBMSO

text: public

characteristic: Submitter Id

text: GENGS-CBMSO

characteristic: broker name

text: Unpaired U2 reads

text: Thermus thermophilus HB27

text: Thermus thermophilus HB27

text: Unpaired U2 reads


text: GENGS-CBMSO

text: public

text: GENGS-CBMSO

text: Paired reads

characteristic: description

text: Thermus thermophilus HB27

text: Thermus thermophilus HB27

text: Paired reads


text: Oesophagus

characteristic: ArrayExpress-Phenotype

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Centre for Structural and Functional Genomics

characteristic: collection date

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

text: Bacterial communities of Canadian Lakes

characteristic: project name

text: metagenome

text: NovaSeq

characteristic: sequencing method

text: Lake sample bacterial DNA

text: water

characteristic: water environmental package


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Centre for Structural and Functional Genomics

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: description

text: Canada

text: metagenome

text: metagenome

text: Bacterial communities of Canadian Lakes

text: metagenome

text: NovaSeq

text: Lake sample bacterial DNA

characteristic: title

text: water


HSV-2 clone_1 pacbio

characteristic: ENA-CHECKLIST

text: CBMSO

text: public

text: HSV-2 clone_1 pacbio

text: Centro de Biología Molecular Severo Ochoa (CSIC-UAM)

characteristic: collecting institution

text: Alberto Domingo Lopez-Muñoz

characteristic: collector name

text: Herpes simplex virus type 2

characteristic: common name

text: plaque-isolated clone 1

text: Spain

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: Human herpesvirus 2 strain 333

text: Human herpesvirus 2 strain 333

text: HSV-2 clone_1 pacbio


HSV-1 [17] wt PB

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CBMSO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: HSV-1 [17] wt PB

text: Institute of Virology, University of Glasgow, Scotland, UK

text: Herpes simplex virus type 1

text: wt virus

text: United Kingdom

text: human

text: healthy

text: Homo sapiens

text: not provided

text: Human alphaherpesvirus 1 strain 17

characteristic: organism

text: Human alphaherpesvirus 1 strain 17

text: HSV-1 [17] wt PB


HSV-1 [17] BAC PB

characteristic: ENA-CHECKLIST

text: CBMSO

text: public

characteristic: INSDC status

characteristic: SRA accession

text: HSV-1 [17] BAC PB

characteristic: Submitter Id

text: Institute of Virology, Hannover Medical School, Hannover, Germany

characteristic: collecting institution

text: Herpes simplex virus type 1

text: BAC-derived virus

characteristic: description

text: Germany

text: human

text: healthy

text: Homo sapiens

text: not provided

text: Human alphaherpesvirus 1 strain 17

text: Human alphaherpesvirus 1 strain 17

text: HSV-1 [17] BAC PB

characteristic: title


HSV-2 [MS] BAC PB

text: CBMSO

text: public

text: HSV-2 [MS] BAC PB

text: Herpes simplex virus type 2

characteristic: common name

text: BAC-derived virus

text: Germany

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

text: healthy

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: MS

text: Human alphaherpesvirus 2

text: Human alphaherpesvirus 2

text: HSV-2 [MS] BAC PB


Alteromonas macleodii EZ55

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Alteromonas macleodii EZ55

tag: Namespace:Institute for Chemistry and Biology of the Marine Environment

text: tropical Pacific

characteristic: broad-scale environmental context

characteristic: collection date

characteristic: depth

text: Prochlorococcus enrichment culture

characteristic: environmental medium

text: Pacific Ocean

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: seawater

characteristic: local environmental context

text: Alteromonas macleodii

text: Pangenomics of Alteromonas macleodii

characteristic: project name

text: Alteromonas macleodii

text: PacBio II

characteristic: sequencing method

text: Pangenomics of Alteromonas macleodii

text: water

characteristic: water environmental package


characteristic: ENA-CHECKLIST

text: YALE SCHOOL OF MEDICINE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:YALE SCHOOL OF MEDICINE

characteristic: Submitter Id

text: human

characteristic: common name

text: High-fat diet

characteristic: description

text: cecum

characteristic: environment (biome)

text: mouse

characteristic: environment (feature)

text: intestinal content

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: human-gut

characteristic: human gut environmental package

text: metagenome

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: shotgun

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: YALE SCHOOL OF MEDICINE

characteristic: INSDC center name

text: public

text: cecum

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: High-carbohydrate diet

text: intestinal content

characteristic: environmental medium

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

text: metagenome

characteristic: investigation type

text: mouse

characteristic: local environmental context

text: Homo sapiens

characteristic: project name

text: Homo sapiens

text: shotgun

characteristic: sequencing method


characteristic: ENA-CHECKLIST

text: YALE SCHOOL OF MEDICINE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: High-fat diet

characteristic: description

text: cecum

characteristic: environment (biome)

text: mouse

characteristic: environment (feature)

text: intestinal content

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

text: human-gut

text: metatranscriptome

text: Homo sapiens

text: Homo sapiens

text: shotgun


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: YALE SCHOOL OF MEDICINE

characteristic: INSDC center name

text: public

text: cecum

characteristic: broad-scale environmental context

characteristic: collection date

text: human

text: High-carbohydrate diet

text: intestinal content

characteristic: environmental medium

text: USA

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metatranscriptome

text: mouse

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: shotgun

characteristic: title


characteristic: ENA-CHECKLIST

text: Hopital Henri Mondor APHP

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Hopital Henri Mondor APHP

characteristic: Submitter Id

text: Stenotrophomonas maltophilia

characteristic: common name

text: tracheal wash

characteristic: description

text: Sarthe - France

characteristic: geographic location (region and locality)

text: Horse

characteristic: host

characteristic: isolate

text: Stenotrophomonas maltophilia

text: Stenotrophomonas maltophilia

text: Respiratory


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Hopital Henri Mondor APHP

characteristic: INSDC center name

text: public

text: Stenotrophomonas maltophilia

text: urine

text: Beauvais - France

text: Human

text: Stenotrophomonas maltophilia

text: Stenotrophomonas maltophilia

text: Urine


characteristic: ENA first public

text: UNIVERSITY COLLEGE DUBLIN

text: public

tag: Namespace:UNIVERSITY COLLEGE DUBLIN

text: cattle

text: 12 h post-CIDR removal

characteristic: group

text: Bos taurus

characteristic: organism

text: Bos taurus

text: Female

characteristic: sex

text: endometrium


characteristic: ENA-CHECKLIST

text: UNIVERSITY COLLEGE DUBLIN

characteristic: INSDC last update

text: public

text: cattle

text: 24 h post-CIDR removal

text: Bos taurus

text: Bos taurus

text: Female

text: endometrium

characteristic: title


text: UNIVERSITY COLLEGE DUBLIN

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: cattle

text: at the onset of oestrus

text: Bos taurus

text: Bos taurus

text: Female

text: endometrium


text: UNIVERSITY COLLEGE DUBLIN

text: public

text: cattle

characteristic: common name

text: 12 h post the onset of oestrus

text: Bos taurus

text: Bos taurus

text: Female

text: endometrium


text: UNIVERSITY COLLEGE DUBLIN

text: public

text: cattle

text: 48 h post the onset of oestrus

text: Bos taurus

text: Bos taurus

text: Female

text: endometrium


text: UNIVERSITY COLLEGE DUBLIN

text: public

text: cattle

text: day 7 after the onset of oestrus (luteal phase)

text: Bos taurus

text: Bos taurus

text: Female

text: endometrium


Dwuxii

text: Quadram Institute Bioscience

characteristic: INSDC first public

text: public

text: Dwuxii

tag: Namespace:Quadram Institute Bioscience

text: human

characteristic: broad-scale environmental context

characteristic: collection date

text: Isolation, genome sequencing, and growth characteristics of a sulphate reducing bacterium belonging to the deltaproteobacteria class (QI0027T) with the ability to fix nitrogen

characteristic: description

text: stool

characteristic: environmental medium

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: stool

characteristic: host body product

text: healthy

characteristic: host disease status

text: human-gut

characteristic: human gut environmental package

characteristic: investigation type

text: human

characteristic: local environmental context

text: Desulfovibrio diazotrophicus

text: Sulphate reducing bacteria isolated from the human gut

characteristic: project name

text: obligate anaerobe

characteristic: relationship to oxygen

text: Desulfovibrio diazotrophicus

text: Illumina HiSeq 3000

characteristic: sequencing method

text: Desulfovibrio diazotrophicus sp. nov., a sulphate reducing bacterium from human gut origin capable of nitrogen fixation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: thale cress

characteristic: common name

text: Col-0. 5-week old leaves were untreated. The sample was collected without any treatment.

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

characteristic: title


text: JIC

characteristic: INSDC first public

text: public

text: thale cress

text: Col-0, 5-week old leaves infiltrated with 10mM MgCl2 mock, sample collected at 4hpi.

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were untreated. The sample was collected without any treatment. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 2nd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were untreated. The sample was collected without any treatment. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi. 3rd

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0. 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: rrs1-3 rrs1b-1 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were untreated. The sample was collected without any treatment. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0), 5-week old leaves were infiltrated with 10mM MgCl2 mock. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-WT in 10mM MgCl2. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: eds1-2 (Col-0). 5-week old leaves were infiltrated with OD600=0.2 Pf0-1:AvrRps4-KRVY135-138AAAAmutant in 10mM MgCl2. The sample was collected at 4hpi. 3rd batch.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0 genomic DNA was treated with Tn5 Nextera enzyme as the normalization control. Repeat 1.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: JIC

text: public

text: thale cress

text: Col-0 genomic DNA was treated with Tn5 Nextera enzyme as the normalization control. Repeat 2.

text: Arabidopsis thaliana

text: Arabidopsis thaliana


text: Quadram Institute Bioscience

text: public

characteristic: collection date

text: DSMZ109475 = NCIMB15228 = NCTC14354

characteristic: culture collection

text: Transcriptomic analyses of Desulfovibrio diazotrophicus QI0027 cells in minimum media with or without bioavailable nitrogen

characteristic: isolate

text: QI0027 was isolated from a faecal sample from healthy individual collected in China.

characteristic: isolation source

text: Quadram Institute Bioscience

characteristic: lab host

text: Desulfovibrio diazotrophicus

text: Desulfovibrio diazotrophicus

text: QI0027 transcriptome with minimum Postgate B media, nitrogen repleted


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Quadram Institute Bioscience

text: public

text: DSMZ109475 = NCIMB15228 = NCTC14354

text: Transcriptomic analyses of Desulfovibrio sp. QI0027 cells in minimum media with or without bioavailable nitrogen

text: Quadram Institute Bioscience

text: Desulfovibrio diazotrophicus

text: Desulfovibrio diazotrophicus

text: QI0027 transcriptome with minimum Postgate B media, nitrogen replete


characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: DSMZ109475 = NCIMB15228 = NCTC14354

text: Quadram Institute Bioscience

text: Desulfovibrio diazotrophicus

text: Desulfovibrio diazotrophicus

text: QI0027 transcriptome with minimum Postgate B media, nitrogen deplete


text: Schistosoma mansoni

characteristic: ArrayExpress-SPECIES

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Schistosoma mansoni

text: Schistosoma mansoni

text: Schistosoma mansoni

text: Schistosoma mansoni


text: Schistosoma curassoni

text: SC

characteristic: INSDC first public

text: public

text: Schistosoma curassoni

characteristic: common name

text: Schistosoma curassoni

characteristic: organism

text: Schistosoma curassoni

text: Schistosoma curassoni

characteristic: title


text: Schistosoma margrebowiei

text: SC

text: public

text: Schistosoma margrebowiei

text: Schistosoma margrebowiei

text: Schistosoma margrebowiei

text: Schistosoma margrebowiei


text: Schistosoma rodhaini

text: SC

text: public

text: Schistosoma rodhaini

text: Schistosoma rodhaini

text: Schistosoma rodhaini

text: Schistosoma rodhaini


text: Schistosoma haematobium

text: SC

text: public

text: Schistosoma haematobium

text: Schistosoma haematobium

text: Schistosoma haematobium

text: Schistosoma haematobium


text: Schistosoma mattheei

text: SC

text: public

text: Schistosoma mattheei

text: Schistosoma mattheei

text: Schistosoma mattheei

text: Schistosoma mattheei


text: Schistosoma guineensis

text: SC

text: public

text: Schistosoma guineensis

text: Schistosoma guineensis

text: Schistosoma guineensis

text: Schistosoma guineensis


text: Schistosoma intercalatum

text: SC

text: public

text: Schistosoma intercalatum

text: Schistosoma intercalatum

text: Schistosoma intercalatum

text: Schistosoma intercalatum


text: Heterobilharzia americana

text: SC

text: public

text: Heterobilharzia americana

text: Heterobilharzia americana

text: Heterobilharzia americana

text: Heterobilharzia americana


text: Dicrocoelium dendriticum

text: SC

text: public

text: Dicrocoelium dendriticum

text: Dicrocoelium dendriticum

text: Dicrocoelium dendriticum

text: Dicrocoelium dendriticum


text: Schistosoma spindale

text: SC

text: public

text: Schistosoma spindale

text: Schistosoma spindale

text: Schistosoma spindale

text: Schistosoma spindale


text: Schistosoma bovis

text: SC

text: public

text: Schistosoma bovis

text: Schistosoma bovis

text: Schistosoma bovis

text: Schistosoma bovis


text: CINVESTAV UNIDAD IRAPUATO

text: public

tag: Namespace:CINVESTAV UNIDAD IRAPUATO

text: 4 days after germination

characteristic: age

text: thale cress

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: control (ethanol plus 30 microM cycloheximide)

characteristic: treatment


characteristic: ENA-CHECKLIST

text: CINVESTAV UNIDAD IRAPUATO

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: induction (10 microM dexamethasone in ethanol plus 30 microM cycloheximide)


characteristic: ENA-FIRST-PUBLIC

text: CINVESTAV UNIDAD IRAPUATO

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: control (ethanol)


characteristic: ENA-LAST-UPDATE

text: CINVESTAV UNIDAD IRAPUATO

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: shoot

text: induction (100 microM dexamethasone in ethanol)


characteristic: External Id

text: CINVESTAV UNIDAD IRAPUATO

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root


text: CINVESTAV UNIDAD IRAPUATO

characteristic: INSDC center name

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root


text: CINVESTAV UNIDAD IRAPUATO

characteristic: INSDC last update

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root

text: control (ethanol)


text: CINVESTAV UNIDAD IRAPUATO

text: public

text: 4 days after germination

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: root


text: Anopheles coluzzii

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Anopheles coluzzii

text: Anopheles coluzzii

text: Anopheles coluzzii


text: Anopheles darlingi

text: SC

text: public

characteristic: INSDC status

text: Anopheles darlingi

text: Anopheles darlingi

text: Anopheles darlingi


text: Anopheles funestus

text: SC

text: public

characteristic: SRA accession

text: African malaria mosquito

text: Anopheles funestus

text: Anopheles funestus


text: Anopheles gambiae

text: SC

text: public

characteristic: Submitter Id

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae


text: Anopheles maculipalpis

text: SC

characteristic: INSDC first public

text: public

text: Anopheles maculipalpis

text: Anopheles maculipalpis

text: Anopheles maculipalpis


text: Homo Sapiens

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: Human


text: Homo Sapiens

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: HUMAN


text: mouse

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus Musculus

characteristic: title


text: Mouse

text: SC

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus Musculus


text: Plasmodium vivax

text: SC

text: public

text: malaria parasite P. vivax

text: Plasmodium vivax

text: Plasmodium vivax


text: public

tag: Namespace:IFOM, the FIRC Institute of Molecular Oncology

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: colorectal cancer cell

text: human

text: Protocols: Primary colonic tissues were obtained from the hospital. Tumoral tissues were processed according to Fujii et al (Fujii et al, 2015) with few modifications. Surgically resected specimens were reduced to a size of 3-5 mm, extensively washed with cold PBS and gentamicin (20microg/ml) and incubated with PBS-EDTA (2,5mM) rocking on a wheel for 1h at 4°C. After PBS-EDTA tissues sample was further washed with cold PBS and tumoral cells were released and collected by centrifuging at 400g 5’ 4°C. Not applicable scRNA-seq libraries were prepared according to 10X library preparation kit.

text: adult

characteristic: developmental stage

text: colorectal cancer

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: colon

characteristic: organism part

text: neoplasm

characteristic: sampling site

text: Homo sapiens

text: female

characteristic: sex


text: Human

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo Sapiens


text: homo sapiens

characteristic: ArrayExpress-SPECIES

text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


text: public

tag: Namespace:Department of Genetics, University of Cambridge, Cambridge, UK

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mature sperm was collected from male ICR mice by modified swim-up method. Two-month-old male offspring from four dietary treatment groups were sacrificed and sperm collected from their cauda epididymis and vas deferens. Specimen was liquefied completely and transferred to a round bottom tube with the medium for 30 minutes at 37°C incubation. Specimen was filtered by a 70μm mesh to remove large somatic tissue. Specimen was transferred to a 14mL round bottom centrifuge tube and was centrifuge the tubes at 300G for 10 minutes at room temperature. Supernatant was removed and the new medium was overlaid on the sperm pellet. Specimen was incubated at a 45°angle for 30 minutes at 37°C for swim-up. The supernatant was collected to the new tube and the new medium was overlaid on the sperm pellet. The tube was centrifuge at 300G for 10 minutes at RT again. Repeat the swim-up process for 4 to 6 times. Sperm from each swim-up cycle was collected together and was centrifuged at 2000G for 5 minutes. After removing the supernatant, the pellet was washed with a 1ml PBS + 0.1% PVA. Sperm was centrifuged at 2000G for 5 minutes. The supernatant was removed and the pellet was re-suspend with 1ml somatic cell lysis buffer (0.1% SDS and 0.5% Triton-X) for 10 minutes on ice to eliminate somatic cell contamination. Then Specimen was centrifuged at 2000G for 5 minutes. The pellet was washed with a 1ml PBS + 0.1% PVA. Resuspend the pellet in a volume of 200 μl of H2O with 4.62 μl of 1M DTT. The sample was separated to two aliquots for gDNA and RNA isolation. Mature sperm collection from their cauda epididymis and vas deferens. Animal was anesthetised with isoflurane before sacrifice.Tissue minced with scalpel then incubated at 37 celsius for 30 minutes - 1 hour. 2ml of sample was passed through the 70um mesh in a 50ml falcon tube using a cut pipette tip. The plate was washed twice with 2ml media each time, media was also passed through the filter into the tube. Then transferred to 14ml round tube with 10ml pipette before centrifuged at 2000rpm for 10 minutes. (Started swim up at 37 celsius for 30 minutes, collected swim up media to a 15 ml falcon, add 4ml HTF to original centrifuge at 2000rpm for 10 minutes) x2 then repeat again but only add 3ml of HFT. 4th swim up was then also collected at 37 celsius for 30 minus and collected into the falcon tube. The tube with swim ups was then centrifuged at 5000 rpm for 7 minutes. Supernatant was aspirated and pellet resuspended with 1ml PBS + 0.01% PVA and then transferred to a siliconized 1.5ml tube. Sample was then centrifuged at 2000rpm for 3 minutes. Somatic cell contamination was removed by a second wash in 750ul of lysis buffer (0.1% SDS and 0.5% triton-X) for 7 minutes and centrifuged at 2000rpm for 3 minutes before washing in 1ml PBS + 0.01% PVA and centrifuged again at 2000rpm for 3 minutes. The pellet was resuspended in 200 ul of water with 4.63 ul of 1 molar DTT. 700 ml of tri reagent added and samples then frozen at -80 celsius. Six- to eight-week-old virgin female ICR mice (Envigo, USA) were caged with ICR males. Pregnancy was dated with vaginal plugs (day 0.5). On pregnancy day 12.5, females were randomly assigned to either a control (C) group or a group subjected to undernutrition (U); there were no differences in weight between C and U mothers before pregnancy or at the time of group assignment. Food intake of U mothers of the 2nd or 3rd cohort was restricted to 22% or 50% of that of controls, respectively, from day 12.5 to 18.5. After delivery, litter size was equalised to 8 in all groups. C and U litters were further distributed into two additional experimental groups: mothers in both control and U groups were provided with either (1) free access to chow after delivery, or (2) 22% or 50% food restriction on a per day basis from the day of delivery until the day of weaning (21 days post partum). We designated these four groups as control-control (CC), undernutrition- control (UC), control-undernutrition (CU) and undernutrition-undernutrition (UU), indicating the prenatal and postnatal experimental condition, respectively. For all groups, pups were weaned at age 3 weeks onto normal chow, to which they had free access. For RNA isolation, 100 μl of the sperm aliquot, 20 μl of 3M NaOAc and 700 μl TRI Reagent (Sigma-Aldrich) were mixed. Then RNA isolation was carried out according to the manufacturer’s instructions with an overnight precipitation step using co-precipitant pink (Bioline BIO-37075) at –20°C. Twelve ng of sperm RNA was treated by DNase I (Sigma-Aldrich) and purified by ethanol precipitation with co-precipitant pink. The quality and quantity of sperm RNAs were tested by Bioanalyzer RNA 6000 Pico Kit (Agilent) and Qubit Fluorometer with microRNA Assay Kit. Ten ng of DNase I treated sperm RNA mixed with Spike-Ins mix from Locati et al 2015 [ Nucleic Acids Res. 2015;43(14):e89. doi:10.1093/nar/gkv303]. Then smRNA libraries were constructed using NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific) according to the manufacturer’s instructions. Twenty cycles of whole-libraries amplification were carried out. Size selection (132 - 200 bp) of the smRNA libraries was performed using BluePippin with 3% agarose gel (Sage Science). The libraries were quantified by Kapa library quantification kit (Roche) and were pooled in equimolar ratio.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: in utero (mother's) carlorie restriciton

text: not applicable

characteristic: isolate

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sperm

unit: percent

characteristic: postnatal calorie restriction

characteristic: sacrifice date

text: Mus musculus

text: male

text: ICR

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Genetics, University of Cambridge, Cambridge, UK

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: adult

characteristic: individual

text: not applicable

text: Mus musculus

text: Mus musculus

text: sperm

characteristic: organism part

text: Mus musculus

text: male

characteristic: sex

text: ICR


characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Epididymis cauda and vas deferens were collected and removed of all fat and blood vessels before transfer to 2ml of warm modified HTF or M2 media on a 5cm plate. After incisions were made in the cauda epididymis and vas deferens, they were gently squeezed to allow the caudal fluid to ooze out into the medium. Mature sperm collection from their cauda epididymis and vas deferens. Animal was anesthetised with isoflurane before sacrifice.Tissue minced with scalpel then incubated at 37 celsius for 30 minutes - 1 hour. 2ml of sample was passed through the 70um mesh in a 50ml falcon tube using a cut pipette tip. The plate was washed twice with 2ml media each time, media was also passed through the filter into the tube. Then transferred to 14ml round tube with 10ml pipette before centrifuged at 2000rpm for 10 minutes. (Started swim up at 37 celsius for 30 minutes, collected swim up media to a 15 ml falcon, add 4ml HTF to original centrifuge at 2000rpm for 10 minutes) x2 then repeat again but only add 3ml of HFT. 4th swim up was then also collected at 37 celsius for 30 minus and collected into the falcon tube. The tube with swim ups was then centrifuged at 5000 rpm for 7 minutes. Supernatant was aspirated and pellet resuspended with 1ml PBS + 0.01% PVA and then transferred to a siliconized 1.5ml tube. Sample was then centrifuged at 2000rpm for 3 minutes. Somatic cell contamination was removed by a second wash in 750ul of lysis buffer (0.1% SDS and 0.5% triton-X) for 7 minutes and centrifuged at 2000rpm for 3 minutes before washing in 1ml PBS + 0.01% PVA and centrifuged again at 2000rpm for 3 minutes. The pellet was resuspended in 200 ul of water with 4.63 ul of 1 molar DTT. 700 ml of tri reagent added and samples then frozen at -80 celsius. Six- to eight-week-old virgin female ICR mice (Envigo, USA) were caged with ICR males. Pregnancy was dated with vaginal plugs (day 0.5). On pregnancy day 12.5, females were randomly assigned to either a control (C) group or a group subjected to undernutrition (U); there were no differences in weight between C and U mothers before pregnancy or at the time of group assignment. Food intake of U mothers of the 2nd or 3rd cohort was restricted to 22% or 50% of that of controls, respectively, from day 12.5 to 18.5. After delivery, litter size was equalised to 8 in all groups. C and U litters were further distributed into two additional experimental groups: mothers in both control and U groups were provided with either (1) free access to chow after delivery, or (2) 22% or 50% food restriction on a per day basis from the day of delivery until the day of weaning (21 days post partum). We designated these four groups as control-control (CC), undernutrition- control (UC), control-undernutrition (CU) and undernutrition-undernutrition (UU), indicating the prenatal and postnatal experimental condition, respectively. For all groups, pups were weaned at age 3 weeks onto normal chow, to which they had free access. For RNA isolation, 100 μl of the sperm aliquot, 20 μl of 3M NaOAc and 700 μl TRI Reagent (Sigma-Aldrich) were mixed. Then RNA isolation was carried out according to the manufacturer’s instructions with an overnight precipitation step using co-precipitant pink (Bioline BIO-37075) at –20°C. Twelve ng of sperm RNA was treated by DNase I (Sigma-Aldrich) and purified by ethanol precipitation with co-precipitant pink. The quality and quantity of sperm RNAs were tested by Bioanalyzer RNA 6000 Pico Kit (Agilent) and Qubit Fluorometer with microRNA Assay Kit. Ten ng of DNase I treated sperm RNA mixed with Spike-Ins mix from Locati et al 2015 [ Nucleic Acids Res. 2015;43(14):e89. doi:10.1093/nar/gkv303]. Then smRNA libraries were constructed using NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific) according to the manufacturer’s instructions. Twenty cycles of whole-libraries amplification were carried out. Size selection (132 - 200 bp) of the smRNA libraries was performed using BluePippin with 3% agarose gel (Sage Science). The libraries were quantified by Kapa library quantification kit (Roche) and were pooled in equimolar ratio.

characteristic: description

text: adult

characteristic: developmental stage

text: not applicable

text: Mus musculus

text: Mus musculus

text: sperm

text: Mus musculus

text: male

text: ICR


text: unknown

characteristic: ArrayExpress-SEX

text: Anopheles bellator

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

text: Unknown

characteristic: GENDER

text: SC

text: public

text: Anopheles bellator

text: Anopheles bellator

characteristic: organism

text: Anopheles bellator

characteristic: title


text: male

text: Anopheles nili

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Male

text: SC

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: Anopheles nili

text: Anopheles nili

text: Anopheles nili


text: female

text: Anopheles marshallii

text: Female

text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Anopheles marshallii

text: Anopheles marshallii

text: Anopheles marshallii


text: female

text: Anopheles gambiae

text: Female

text: SC

text: public

characteristic: Submitter Id

text: African malaria mosquito

characteristic: common name

text: Anopheles gambiae

text: Anopheles gambiae


text: male

text: Anopheles gambiae

text: Male

text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae


text: male

text: Anopheles funestus

text: Male

text: SC

text: public

text: African malaria mosquito

text: Anopheles funestus

text: Anopheles funestus


text: female

text: Anopheles funestus

text: Female

text: SC

text: public

text: African malaria mosquito

text: Anopheles funestus

text: Anopheles funestus


text: female

text: Anopheles coustani

text: Female

text: SC

text: public

text: Anopheles coustani

text: Anopheles coustani

text: Anopheles coustani


text: unknown

text: Anopheles cruzii

text: Unknown

text: SC

text: public

text: Anopheles cruzii

text: Anopheles cruzii

text: Anopheles cruzii


text: female

text: Anopheles oryzalimnetes

text: Female

text: SC

text: public

text: Anopheles oryzalimnetes

text: Anopheles oryzalimnetes

text: Anopheles oryzalimnetes


text: female

text: Anopheles coluzzii

text: Female

text: SC

text: public

text: Anopheles coluzzii

text: Neoverruca sp. Okinawa

text: Neoverruca sp. Okinawa


text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Mature sperm was collected from male ICR mice by modified swim-up method. Two-month-old male offspring from four dietary treatment groups were sacrificed and sperm collected from their cauda epididymis and vas deferens. Specimen was liquefied completely and transferred to a round bottom tube with the medium for 30 minutes at 37°C incubation. Specimen was filtered by a 70μm mesh to remove large somatic tissue. Specimen was transferred to a 14mL round bottom centrifuge tube and was centrifuge the tubes at 300G for 10 minutes at room temperature. Supernatant was removed and the new medium was overlaid on the sperm pellet. Specimen was incubated at a 45°angle for 30 minutes at 37°C for swim-up. The supernatant was collected to the new tube and the new medium was overlaid on the sperm pellet. The tube was centrifuge at 300G for 10 minutes at RT again. Repeat the swim-up process for 4 to 6 times. Sperm from each swim-up cycle was collected together and was centrifuged at 2000G for 5 minutes. After removing the supernatant, the pellet was washed with a 1ml PBS + 0.1% PVA. Sperm was centrifuged at 2000G for 5 minutes. The supernatant was removed and the pellet was re-suspend with 1ml somatic cell lysis buffer (0.1% SDS and 0.5% Triton-X) for 10 minutes on ice to eliminate somatic cell contamination. Then Specimen was centrifuged at 2000G for 5 minutes. The pellet was washed with a 1ml PBS + 0.1% PVA. Resuspend the pellet in a volume of 200 μl of H2O with 4.62 μl of 1M DTT. The sample was separated to two aliquots for gDNA and RNA isolation. Mature sperm collection from their cauda epididymis and vas deferens. Animal was anesthetised with isoflurane before sacrifice.Tissue minced with scalpel then incubated at 37 celsius for 30 minutes - 1 hour. 2ml of sample was passed through the 70um mesh in a 50ml falcon tube using a cut pipette tip. The plate was washed twice with 2ml media each time, media was also passed through the filter into the tube. Then transferred to 14ml round tube with 10ml pipette before centrifuged at 2000rpm for 10 minutes. (Started swim up at 37 celsius for 30 minutes, collected swim up media to a 15 ml falcon, add 4ml HTF to original centrifuge at 2000rpm for 10 minutes) x2 then repeat again but only add 3ml of HFT. 4th swim up was then also collected at 37 celsius for 30 minus and collected into the falcon tube. The tube with swim ups was then centrifuged at 5000 rpm for 7 minutes. Supernatant was aspirated and pellet resuspended with 1ml PBS + 0.01% PVA and then transferred to a siliconized 1.5ml tube. Sample was then centrifuged at 2000rpm for 3 minutes. Somatic cell contamination was removed by a second wash in 750ul of lysis buffer (0.1% SDS and 0.5% triton-X) for 7 minutes and centrifuged at 2000rpm for 3 minutes before washing in 1ml PBS + 0.01% PVA and centrifuged again at 2000rpm for 3 minutes. The pellet was resuspended in 200 ul of water with 4.63 ul of 1 molar DTT. 700 ml of tri reagent added and samples then frozen at -80 celsius. Six- to eight-week-old virgin female ICR mice (Envigo, USA) were caged with ICR males. Pregnancy was dated with vaginal plugs (day 0.5). On pregnancy day 12.5, females were randomly assigned to either a control (C) group or a group subjected to undernutrition (U); there were no differences in weight between C and U mothers before pregnancy or at the time of group assignment. Food intake of U mothers of the 2nd or 3rd cohort was restricted to 22% or 50% of that of controls, respectively, from day 12.5 to 18.5. After delivery, litter size was equalised to 8 in all groups. C and U litters were further distributed into two additional experimental groups: mothers in both control and U groups were provided with either (1) free access to chow after delivery, or (2) 22% or 50% food restriction on a per day basis from the day of delivery until the day of weaning (21 days post partum). We designated these four groups as control-control (CC), undernutrition- control (UC), control-undernutrition (CU) and undernutrition-undernutrition (UU), indicating the prenatal and postnatal experimental condition, respectively. For all groups, pups were weaned at age 3 weeks onto normal chow, to which they had free access. Sperm was digested in Proteinase K lysis buffer (10mM Tris-HCl pH8.0, 10 mM EDTA, 100 mM NaCl, 1% SDS, 80 mM DTT, 200 ng/mL Proteinase K). Then gDNA was extracted by phenol/chloroform-based DNA extraction method. To accurately quantify sperm DNA concentration prior to MeDIP, Qubit Fluorometer (Thermo Fisher Scientific) was used according to the manufacturer’s instructions NEBNext DNA library prep Master Mix Set for Illumina (NEB) and Bioo Scientific NEXTflex DNA Barcodes (Bioo Scientific) were used for library preparation according to the manufacturer’s instructions. 1 μg of 5-mC monoclonal antibody (diagnode C15200006 Lot #: GF-005) was used to 0.5 μg of sonicated gDNA (200bp). Twelve cycles of whole-libraries amplification were carried out using Phusion High-Fidelity Taq polymerase. The libraries were quantified by Kapa library quantification kit (Roche) and were pooled in equimolar ratio.

characteristic: description

text: adult

characteristic: developmental stage

characteristic: in utero (mother's) carlorie restriciton

characteristic: individual

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: sperm

characteristic: organism part

characteristic: postnatal calorie restriction

characteristic: sacrafice date

text: Mus musculus

text: male

characteristic: sex

text: ICR

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: house mouse

text: Protocols: Epididymis cauda and vas deferens were collected and removed of all fat and blood vessels before transfer to 2ml of warm modified HTF or M2 media on a 5cm plate. After incisions were made in the cauda epididymis and vas deferens, they were gently squeezed to allow the caudal fluid to ooze out into the medium. Mature sperm collection from their cauda epididymis and vas deferens. Animal was anesthetised with isoflurane before sacrifice.Tissue minced with scalpel then incubated at 37 celsius for 30 minutes - 1 hour. 2ml of sample was passed through the 70um mesh in a 50ml falcon tube using a cut pipette tip. The plate was washed twice with 2ml media each time, media was also passed through the filter into the tube. Then transferred to 14ml round tube with 10ml pipette before centrifuged at 2000rpm for 10 minutes. (Started swim up at 37 celsius for 30 minutes, collected swim up media to a 15 ml falcon, add 4ml HTF to original centrifuge at 2000rpm for 10 minutes) x2 then repeat again but only add 3ml of HFT. 4th swim up was then also collected at 37 celsius for 30 minus and collected into the falcon tube. The tube with swim ups was then centrifuged at 5000 rpm for 7 minutes. Supernatant was aspirated and pellet resuspended with 1ml PBS + 0.01% PVA and then transferred to a siliconized 1.5ml tube. Sample was then centrifuged at 2000rpm for 3 minutes. Somatic cell contamination was removed by a second wash in 750ul of lysis buffer (0.1% SDS and 0.5% triton-X) for 7 minutes and centrifuged at 2000rpm for 3 minutes before washing in 1ml PBS + 0.01% PVA and centrifuged again at 2000rpm for 3 minutes. The pellet was resuspended in 200 ul of water with 4.63 ul of 1 molar DTT. 700 ml of tri reagent added and samples then frozen at -80 celsius. Six- to eight-week-old virgin female ICR mice (Envigo, USA) were caged with ICR males. Pregnancy was dated with vaginal plugs (day 0.5). On pregnancy day 12.5, females were randomly assigned to either a control (C) group or a group subjected to undernutrition (U); there were no differences in weight between C and U mothers before pregnancy or at the time of group assignment. Food intake of U mothers of the 2nd or 3rd cohort was restricted to 22% or 50% of that of controls, respectively, from day 12.5 to 18.5. After delivery, litter size was equalised to 8 in all groups. C and U litters were further distributed into two additional experimental groups: mothers in both control and U groups were provided with either (1) free access to chow after delivery, or (2) 22% or 50% food restriction on a per day basis from the day of delivery until the day of weaning (21 days post partum). We designated these four groups as control-control (CC), undernutrition- control (UC), control-undernutrition (CU) and undernutrition-undernutrition (UU), indicating the prenatal and postnatal experimental condition, respectively. For all groups, pups were weaned at age 3 weeks onto normal chow, to which they had free access. Sperm was digested in Proteinase K lysis buffer (10mM Tris-HCl pH8.0, 10 mM EDTA, 100 mM NaCl, 1% SDS, 80 mM DTT, 200 ng/mL Proteinase K). Then gDNA was extracted by phenol/chloroform-based DNA extraction method. To accurately quantify sperm DNA concentration prior to MeDIP, Qubit Fluorometer (Thermo Fisher Scientific) was used according to the manufacturer’s instructions NEBNext DNA library prep Master Mix Set for Illumina (NEB) and Bioo Scientific NEXTflex DNA Barcodes (Bioo Scientific) were used for library preparation according to the manufacturer’s instructions. 1 μg of 5-mC monoclonal antibody (diagnode C15200006 Lot #: GF-005) was used to 0.5 μg of sonicated gDNA (200bp). Twelve cycles of whole-libraries amplification were carried out using Phusion High-Fidelity Taq polymerase. The libraries were quantified by Kapa library quantification kit (Roche) and were pooled in equimolar ratio.

text: adult

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: sperm

text: Mus musculus

text: male

text: ICR

characteristic: title


text: male

characteristic: ArrayExpress-SEX

text: mouse gut metagenome

characteristic: ArrayExpress-SPECIES

text: Male

characteristic: GENDER

text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: mouse gut metagenome

characteristic: common name

text: mouse gut metagenome

text: mouse gut metagenome

text: mouse gut metagenome


text: female

text: mouse gut metagenome

text: Female

text: SC

text: public

text: mouse gut metagenome

text: mouse gut metagenome

text: mouse gut metagenome

text: mouse gut metagenome


text: AALBORG UNIVERSITY

text: public

tag: Namespace:AALBORG UNIVERSITY

characteristic: day

text: metagenome

text: A

characteristic: replicate

text: B

text: Biofilm

text: metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: C

text: Biofilm

text: metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: AALBORG UNIVERSITY

text: public

text: metagenome

text: B

text: C

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: metagenome

text: C

text: C

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

characteristic: organism

text: A

text: D

text: Biofilm

text: metagenome

characteristic: title


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: B

text: D

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: E

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: B

text: E

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: A

text: Digester

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: C

text: Digester

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: D

text: Digester

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: A

text: E

text: Digester

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: D

text: B

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: E

text: B

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: D

text: D

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: D

text: E

text: Biofilm

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: D

text: B

text: Digester

text: metagenome


text: AALBORG UNIVERSITY

text: public

text: metagenome

text: D

text: Control

text: A

text: Biofilm

text: metagenome


text: Escherichia coli

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: Escherichia coli

text: Escherichia coli

text: Escherichia coli


text: Campylobacter jejuni

text: Campylobacter

text: SC

text: public

text: Campylobacter jejuni

text: Campylobacter jejuni

text: Campylobacter jejuni


text: Sarcophilus harrisii

text: Tasmanian devil

text: SC

text: public

text: BLANK

text: Tasmanian devil

text: Sarcophilus harrisii

text: Sarcophilus harrisii

text: BLANK


text: public

tag: Namespace:Zurich University of Applied Sciences (ZHAW), School of Life Sciences and Facility Management, Institute of Natural Resource Sciences

text: not provided

characteristic: collection date

text: Negative control (PCR 1)

characteristic: description

text: control treatment

characteristic: environment (biome)

text: control treatment

characteristic: environment (feature)

text: control treatment

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: metagenome

text: For bacteria: initial denaturation:95degC_5min; denaturation:98degC_20s; annealing:54degC_15s; extension:72degC_15s; final extension:72degC_5min; cycles:24. For archaea: initial denaturation:95degC_5min; denaturation:98degC_20s; annealing:53degC_15s; extension:72degC_15s; final extension:72degC_5min; cycles:25

characteristic: pcr conditions

text: 27F and 534R for bacteria, Arch516F and Univ806R for archae

characteristic: pcr primers

text: Microbial diversity across compartments of an aquaponic system and their connection to the nitrogen cycle

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: source material identifiers

text: 16S rRNA gene

characteristic: target gene

text: V1-V3 for bacteria, V4 for archae

characteristic: target subfragment

text: Negative control (PCR 1)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Zurich University of Applied Sciences (ZHAW), School of Life Sciences and Facility Management, Institute of Natural Resource Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

characteristic: collection date

text: Negative control (PCR 1)

text: control treatment

text: control treatment

text: control treatment

text: Switzerland

text: not provided

text: not provided

text: metagenome

characteristic: organism

text: metagenome

text: Illumina MiSeq

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Negative control (PCR 1)

characteristic: title


text: public

text: not provided

text: Negative control (PCR 2)

characteristic: description

text: control treatment

characteristic: environment (biome)

text: control treatment

characteristic: environment (feature)

text: control treatment

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: pcr conditions

characteristic: pcr primers

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Negative control (PCR 2)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

text: Negative control (pipet control)

text: control treatment

text: control treatment

text: control treatment

text: Switzerland

text: not provided

text: not provided

text: Qiagen DNeasy PowerSoil Kit

characteristic: nucleic acid extraction

text: metagenome

text: metagenome

text: Illumina MiSeq

characteristic: source material identifiers

text: 16S rRNA gene

characteristic: target gene

text: V1-V3 for bacteria, V4 for archae

characteristic: target subfragment

text: Negative control (pipet control)


text: public

text: not provided

characteristic: collection date

text: Negative control (H2O control)

characteristic: description

text: control treatment

characteristic: environment (biome)

text: control treatment

characteristic: environment (feature)

text: control treatment

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

characteristic: investigation type

characteristic: miscellaneous environmental package

text: Qiagen DNeasy PowerSoil Kit

text: metagenome

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: metagenome

text: Illumina MiSeq

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Negative control (H2O control)

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not provided

text: Negative control (ddH2O control)

text: control treatment

text: control treatment

text: control treatment

text: Switzerland

text: not provided

text: not provided

text: Qiagen DNeasy PowerSoil Kit

characteristic: nucleic acid extraction

text: metagenome

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: source material identifiers

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Negative control (ddH2O control)


text: public

unit: mg/L

characteristic: calcium

characteristic: chloride

characteristic: collection date

text: Fish tank surface biofilm

characteristic: description

text: aquaponic system

characteristic: environment (biome)

text: finfish farming process

characteristic: environment (feature)

text: biofilm material

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

text: 47.2172 N

characteristic: geographic location (latitude)

text: 8.6816 E

characteristic: geographic location (longitude)

characteristic: investigation type

characteristic: miscellaneous environmental package

text: Qiagen DNeasy PowerSoil Kit

text: metagenome

characteristic: organism

characteristic: pH

characteristic: pcr conditions

text: immediately placed into a polystyrene box with cooling elements until the end of the sampling process and then stored at -20°C until further analysis

characteristic: sample material processing

unit: day

characteristic: sample storage duration

unit: °C

characteristic: sample storage temperature

text: metagenome

text: Illumina MiSeq

characteristic: sulfate

text: 16S rRNA gene

characteristic: target gene

text: V1-V3 for bacteria, V4 for archae

characteristic: target subfragment

unit: ºC

characteristic: temperature

text: Fish tank surface biofilm

text: tilapia and lettuce

characteristic: user field 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: calcium

characteristic: chloride

characteristic: collection date

text: Biocarrier biofilm

characteristic: description

text: aquaponic system

characteristic: environment (biome)

text: biofilter

characteristic: environment (feature)

text: biofilm material

characteristic: environment (material)

text: Switzerland

text: 47.2172 N

text: 8.6816 E

text: Qiagen DNeasy PowerSoil Kit

characteristic: nucleic acid extraction

text: metagenome

characteristic: pcr primers

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: source material identifiers

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Biocarrier biofilm

characteristic: title

text: tilapia and lettuce


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Sump surface biofilm

text: aquaponic system

text: pump system

text: biofilm material

text: Switzerland

characteristic: geographic location (country and/or sea)

text: 47.2172 N

characteristic: geographic location (latitude)

text: 8.6816 E

characteristic: geographic location (longitude)

characteristic: investigation type

characteristic: miscellaneous environmental package

text: Qiagen DNeasy PowerSoil Kit

text: metagenome

characteristic: organism

characteristic: pH

characteristic: pcr conditions

characteristic: sample material processing

characteristic: sample storage duration

characteristic: sample storage temperature

text: metagenome

text: Illumina MiSeq

characteristic: sulfate

text: 16S rRNA gene

characteristic: target gene

text: V1-V3 for bacteria, V4 for archae

characteristic: target subfragment

characteristic: temperature

text: Sump surface biofilm

text: tilapia and lettuce

characteristic: user field 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: calcium

characteristic: chloride

characteristic: collection date

text: Hydroponic table surface biofilm

characteristic: description

text: aquaponic system

characteristic: environment (biome)

text: hydroponic cultivation

characteristic: environment (feature)

text: biofilm material

characteristic: environment (material)

text: Switzerland

text: 47.2172 N

text: 8.6816 E

text: Qiagen DNeasy PowerSoil Kit

characteristic: nucleic acid extraction

text: metagenome

characteristic: pcr primers

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: source material identifiers

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Hydroponic table surface biofilm

characteristic: title

text: tilapia and lettuce


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Roots

text: aquaponic system

text: plant roots

text: root matter

text: Switzerland

characteristic: geographic location (country and/or sea)

text: 47.2172 N

characteristic: geographic location (latitude)

text: 8.6816 E

characteristic: geographic location (longitude)

characteristic: investigation type

characteristic: miscellaneous environmental package

text: Qiagen DNeasy PowerSoil Kit

text: metagenome

characteristic: organism

characteristic: pH

characteristic: pcr conditions

characteristic: sample material processing

characteristic: sample storage duration

characteristic: sample storage temperature

text: metagenome

text: Illumina MiSeq

characteristic: sulfate

text: 16S rRNA gene

characteristic: target gene

text: V1-V3 for bacteria, V4 for archae

characteristic: target subfragment

characteristic: temperature

text: Roots

text: tilapia and lettuce

characteristic: user field 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Fish feces

characteristic: description

text: aquaponic system

characteristic: environment (biome)

text: feces

characteristic: environment (feature)

text: fecal material

characteristic: environment (material)

text: Switzerland

text: 47.2172 N

text: 8.6816 E

text: Qiagen DNeasy PowerSoil Kit

characteristic: nucleic acid extraction

text: metagenome

characteristic: pcr primers

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

characteristic: source material identifiers

text: 16S rRNA gene

text: V1-V3 for bacteria, V4 for archae

text: Fish feces

characteristic: title

text: tilapia and lettuce