characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Queensland

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: tolid


status: PUBLIC

tag: attribute

text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

text: UNIVERSITY OF SYDNEY | SANGER INSTITUTE, UNIVERSITY OF CAMBRIDGE

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

text: Queensland

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: project name

characteristic: sample same as

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Queensland

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: tolid


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (latitude)

text: Western Australia

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: project name

characteristic: sample same as

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Victoria

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: South Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: LEG

characteristic: sample derived from

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: South Australia

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: LEG

characteristic: organism part

characteristic: project name

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: South Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: sample same as

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: South Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Australia

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: LEG

characteristic: organism part

characteristic: sample derived from

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: LEG

characteristic: project name

text: unidentified

text: FEMALE

characteristic: sex

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: Queensland

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Queensland

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: unidentified

text: FEMALE

characteristic: sex

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Victoria

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: Victoria

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: South Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: New South Wales

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: not collected

text: unidentified

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: unidentified

text: NOT COLLECTED

characteristic: sex

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

text: unidentified

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: title


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

text: New South Wales

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: New South Wales

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Queensland

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: project name

characteristic: sample same as

text: unidentified

text: FEMALE

characteristic: sex

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Queensland

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: title


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

characteristic: project name

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Victoria

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: sample same as

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Victoria

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: symbiont

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: South Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

characteristic: organism

text: LEG

characteristic: sample derived from

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Queensland

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: LEG

characteristic: organism part

characteristic: project name

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: New South Wales

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: not collected

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

characteristic: sample same as

text: unidentified

text: NOT COLLECTED

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: symbiont


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: New South Wales

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: New South Wales

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

characteristic: project name

characteristic: sample same as

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Queensland

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: symbiont


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Victoria

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Queensland

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: MALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: symbiont


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

text: Western Australia

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: ToL

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (longitude)

text: South Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: New South Wales

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Queensland

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: sample same as

text: unidentified

text: FEMALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: symbiont


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: Queensland

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Western Australia

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: tolid


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: project name

characteristic: sample same as

text: unidentified

text: FEMALE

text: NOT APPLICABLE

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: South Australia

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

text: unidentified

text: FEMALE

characteristic: sex

text: NOT APPLICABLE

text: N

characteristic: symbiont


text: Sanger Institute

characteristic: GAL

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collecting institution

text: Australia

text: Victoria

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

characteristic: lifestage

text: unidentified

characteristic: organism

text: WHOLE ORGANISM

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N

characteristic: title


text: Sanger Institute

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: JOSEPH SCHUBERT | JONAH WALKER

text: Australia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Australia

characteristic: geographic location (region and locality)

text: NOT PROVIDED

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: juvenile

text: unidentified

text: WHOLE ORGANISM

characteristic: organism part

text: unidentified

text: NOT COLLECTED

text: NOT APPLICABLE

text: N

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: JOSEPH SCHUBERT | JONAH WALKER

characteristic: collection date

text: Australia

characteristic: geographic location (longitude)

text: New South Wales

text: NOT PROVIDED

characteristic: habitat

text: JOSEPH SCHUBERT

text: UNIVERSITY OF SYDNEY

text: adult

text: unidentified

text: WHOLE ORGANISM

characteristic: project name

characteristic: sample same as

text: unidentified

text: MALE

text: NOT APPLICABLE

text: N


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Tobias Uller

text: University of Lund, Sweden

characteristic: collecting institution

text: Common wall lizard

characteristic: common name

text: Italy

text: Liguria | Noli

text: urban

text: Tobias Uller

text: University of Lund, Sweden

text: adult

characteristic: lifestage

text: Podarcis muralis

text: WHOLE ORGANISM

text: ToL

text: Podarcis muralis

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N

characteristic: symbiont


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Miguel A. Carretero

text: University of Porto, Portugal

text: Portugal

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Vila do Conde

characteristic: geographic location (region and locality)

text: urban

text: Miguel A. Carretero

text: University of Porto, Portugal

text: adult

text: Podarcis bocagei

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: Podarcis bocagei

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: Javier Abalos

text: University of Lund, Sweden

characteristic: collection date

text: Morocco

characteristic: geographic location (longitude)

text: Oukaimeden

text: sparsely vegetated land, domestic garden (Eunis classification)

characteristic: habitat

text: Javier Abalos

text: University of Lund, Sweden

text: adult

text: Podarcis vaucheri

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: Podarcis vaucheri

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: tolid


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Kinsey Brock

text: University of California, Berkeley, USA

characteristic: collecting institution

text: Greece

text: Andros

text: Kinsey Brock

text: University of California, Berkeley, USA

text: adult

characteristic: lifestage

text: Podarcis erhardii

text: WHOLE ORGANISM

text: ToL

text: Podarcis erhardii

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N


text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Tobias Uller

text: University of Lund, Sweden

text: Italian wall lizard

characteristic: common name

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Tuscany | Massa Marittima

characteristic: geographic location (region and locality)

text: Tobias Uller

text: University of Lund, Sweden

text: adult

text: Podarcis siculus

text: WHOLE ORGANISM

text: ToL

text: Podarcis siculus

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: Raoul van Damme

text: University of Antwerp, Bergium

characteristic: collection date

text: Croatia

characteristic: geographic location (longitude)

text: Vis

text: Mediterranenan maquis

text: Raoul van Damme

text: University of Antwerp, Bergium

text: adult

text: Podarcis melisellensis

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: Podarcis melisellensis

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: title


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Tobias Uller

text: University of Lund, Sweden

characteristic: collecting institution

text: Common wall lizard

text: Italy

text: Rome | Albano Laziale

text: urban

characteristic: habitat

text: Tobias Uller

text: University of Lund, Sweden

text: adult

text: Podarcis muralis

text: WHOLE ORGANISM

text: ToL

characteristic: project name

text: Podarcis muralis

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: tolid


text: Sanger Institute

text: Pmuralis SA NL38

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Tobias Uller

text: University of Lund, Sweden

text: Common wall lizard

characteristic: common name

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Liguria | Noli

text: urban

text: Tobias Uller

text: University of Lund, Sweden

text: adult

characteristic: lifestage

text: Podarcis muralis

text: TERMINAL BODY

characteristic: sample derived from

text: Podarcis muralis

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Pbocagei PB4

text: Tree of Life Programme

text: public

text: Miguel A. Carretero

text: University of Porto, Portugal

characteristic: collection date

text: Portugal

characteristic: geographic location (longitude)

text: Vila do Conde

characteristic: geographic location (region and locality)

text: urban

text: Miguel A. Carretero

text: University of Porto, Portugal

text: adult

text: Podarcis bocagei

characteristic: organism

text: TERMINAL BODY

characteristic: organism part

text: Podarcis bocagei

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont


text: Sanger Institute

characteristic: GAL

text: Pvaucheri OU L31

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Javier Abalos

text: University of Lund, Sweden

characteristic: collecting institution

text: Morocco

text: Oukaimeden

characteristic: habitat

text: Javier Abalos

text: University of Lund, Sweden

text: adult

text: Podarcis vaucheri

text: TERMINAL BODY

characteristic: project name

text: Podarcis vaucheri

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: title

characteristic: tolid


text: Sanger Institute

text: Perhardii AB

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Kinsey Brock

text: University of California, Berkeley, USA

text: Greece

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Andros

text: Kinsey Brock

text: University of California, Berkeley, USA

text: adult

characteristic: lifestage

text: Podarcis erhardii

text: TERMINAL BODY

characteristic: sample derived from

text: Podarcis erhardii

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Ptiliguerta D300

text: Tree of Life Programme

text: public

text: Daniele Salvi

text: University of L'Aquila, Italy

characteristic: collection date

text: Italy

characteristic: geographic location (longitude)

text: Sardinia | Punta Falcone

characteristic: geographic location (region and locality)

text: Mediterranenan maquis

text: Daniele Salvi

text: University of L'Aquila, Italy

text: adult

text: Podarcis tiliguerta

characteristic: organism

text: TERMINAL BODY

characteristic: organism part

text: Podarcis tiliguerta

text: FEMALE

text: NOT PROVIDED

text: N


text: Sanger Institute

characteristic: GAL

text: Pvaucheri OU G84

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Javier Abalos

text: University of Lund, Sweden

characteristic: collecting institution

text: Morocco

text: Oukaimeden

characteristic: habitat

text: Javier Abalos

text: University of Lund, Sweden

text: adult

text: Podarcis vaucheri

text: TERMINAL BODY

characteristic: project name

text: Podarcis vaucheri

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont


text: Sanger Institute

text: Pmuralis SA NL22

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Tobias Uller

text: University of Lund, Sweden

text: Common wall lizard

characteristic: common name

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Liguria | Noli

text: urban

text: Tobias Uller

text: University of Lund, Sweden

text: adult

characteristic: lifestage

text: Podarcis muralis

text: TERMINAL BODY

text: Podarcis muralis

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: title

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Psiculus 01

text: Tree of Life Programme

text: public

text: Tobias Uller

text: University of Lund, Sweden

characteristic: collection date

text: Italian wall lizard

text: Italy

characteristic: geographic location (longitude)

text: Tuscany | Massa Marittima

characteristic: geographic location (region and locality)

text: Tobias Uller

text: University of Lund, Sweden

text: adult

text: Podarcis siculus

characteristic: organism

text: TERMINAL BODY

characteristic: sample derived from

text: Podarcis siculus

text: FEMALE

characteristic: sex

text: NOT PROVIDED

text: N


text: Sanger Institute

characteristic: GAL

text: Pmelisellensis 2

text: Tree of Life Programme

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Raoul van Damme

text: University of Antwerp, Bergium

characteristic: collecting institution

text: Croatia

text: Vis

text: Mediterranenan maquis

characteristic: habitat

text: Raoul van Damme

text: University of Antwerp, Bergium

text: adult

text: Podarcis melisellensis

text: TERMINAL BODY

characteristic: organism part

characteristic: project name

text: Podarcis melisellensis

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: symbiont


text: Sanger Institute

text: Pmuralis nig AZ G58

text: Tree of Life Programme

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Tobias Uller

text: University of Lund, Sweden

text: Common wall lizard

characteristic: common name

text: Italy

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Rome | Albano Laziale

text: urban

text: Tobias Uller

text: University of Lund, Sweden

text: adult

characteristic: lifestage

text: Podarcis muralis

text: TERMINAL BODY

text: Podarcis muralis

text: FEMALE

text: NOT PROVIDED

text: N

characteristic: title

characteristic: tolid


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Institute of Ecology and Earth Sciences

text: public

text: Soil from oak dominated forest planation

characteristic: broad-scale environmental context

characteristic: collection date

unit: m

characteristic: depth

characteristic: elevation

text: root

characteristic: environmental medium

text: Netherlands

characteristic: geographic location (longitude)

text: Mesocosm

characteristic: local environmental context

text: metagenome

characteristic: organism

text: Biotic and abiotic effects on tree growth and aboveground herbivory during early afforestation

text: metagenome


text: Institute of Ecology and Earth Sciences

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: soil

text: Netherlands

text: Mesocosm

text: metagenome

characteristic: project name

text: metagenome


text: Sanger Institute

characteristic: GAL

text: NOT PROVIDED

text: Tree of Life Programme

text: public

text: Ruiqi Li

text: UNIVERSITY OF COLORADO BOULDER

characteristic: collecting institution

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Florida | West Palm Beach

characteristic: geographic location (region and locality)

text: Sandy bottom near reefs

characteristic: habitat

text: Ruiqi Li

text: UNIVERSITY OF COLORADO BOULDER

text: adult

characteristic: lifestage

text: Americardia media

text: WHOLE ORGANISM

characteristic: organism part

text: ToL

text: Americardia media

text: HERMAPHRODITE MONOECIOUS

characteristic: sex

text: N

characteristic: symbiont

characteristic: title


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Sanger Institute

text: Tree of Life Programme

text: public

text: Ruiqi Li

text: UNIVERSITY OF COLORADO BOULDER

characteristic: collection date

characteristic: depth

text: USA

characteristic: geographic location (longitude)

text: Florida | West Palm Beach

text: Sandy bottom near reefs

text: Ruiqi Li

text: UNIVERSITY OF COLORADO BOULDER

text: adult

text: Americardia media

characteristic: organism

text: **OTHER SOMATIC ANIMAL TISSUE**

characteristic: sample derived from

text: Americardia media

text: HERMAPHRODITE MONOECIOUS

text: N

characteristic: tolid


text: MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: DNA of Recombinant inbred line (mixed-stage population) between RSA076 and RSC011

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Pristionchus pacificus

text: Pristionchus pacificus


text: Respiratory Virus Unit, Microbiology Services Colindale, Public Health England

text: public

text: COVID-19 Genomics UK Consortium

characteristic: broker name

characteristic: collecting institution

text: not provided

characteristic: collector name

text: not provided

characteristic: definition for seropositive sample

text: United Kingdom

text: England

characteristic: geographic location (region and locality)

text: not provided

characteristic: host common name

text: not provided

characteristic: host health state

text: Human

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: receipt date

text: active surveillance in response to outbreak

characteristic: sample capture status

text: not provided

characteristic: serotype (required for a seropositive sample)

text: not provided

characteristic: virus identifier


characteristic: ENA first public

characteristic: ENA-CHECKLIST

text: Anthony Smith, NICD

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: EnteroBase

text: EnteroBase

text: A. Smith, National Institute for Communicable Diseases

characteristic: collection date

text: Whole genome sequencing data uploaded by EnteroBase user

characteristic: description

text: South Africa

text: Gauteng

text: Human, Blood culture

text: Salmonella enterica

characteristic: organism

text: Salmonella enterica

text: Typhi

characteristic: serovar

characteristic: strain

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: DKFZ

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Oropharyngeal metagenomic sample

text: human nasopharyngeal metagenome

text: human nasopharyngeal metagenome


text: public

tag: Namespace:University of Cambridge, The Gurdon Institute

text: ArrayExpress

characteristic: broker name

text: osteoblast

text: human

characteristic: common name

text: Protocols: 60,000 AID-DIvA cells were seeded into 6-well plates, incubated for 24 hours and then transfected as described earlier. Transfected cells were incubated for 24 hours, their media was then replaced, and they were then incubated for a further 48 hours. Cells were then treated with or without 300nM 4-hydroxytamoxifen (OHT) for 4 hours to induce DSBs, then washed twice with pre-warmed PBS and then once with pre-warmed media containing 500μg/mL IAA, and then replaced with media containing 500μg/mL IAA and incubated for 14 hours to degrade the AsiSI fusion protein and allow complete DSB repair. ATMi (KU-55933, Merck SML1109), ATRi (VE-821, Merck SML1415) and DNA-PKi (NU7441, Tocris 3712) were all used at 10μM and administered in a 1 hour pre-treatment and maintained throughout the period of the experiment. Cells were placed on ice, washed once in PBS and lysed in cytoplasmic lysis buffer (50mM HEPES pH7.9, 10mM KCl2 , 1.5mM MgCl2, 0.34M sucrose, 0.5% triton, 10% glycerol, 1mM DTT) for 10 minutes. Cells were then washed once in cytoplasmic lysis buffer and then the nuclei were lysed in genomic extraction buffer (50mM Tris pH 8.0, 5mM EDTA, 1% SDS, 0.5mg/mL Proteinase K). The nuclear lysates were transferred tor 2mL DNA LoBind microcentrifuge tubes (Fisher Scientific, 0030108426) and incubated in a thermomixer at 60°C for 40 minutes with 500rpm agitation. 0.1 volumes of 3M sodium acetate pH 5.2 was then added followed by 2.5 volumes of 100% ethanol, the tubes were inverted several times to mix an then incubated on ice for 1 hour to precipitate the genomic DNA. The DNA was then pelleted at 19000g for 20 minutes at 4°C, washed in 75% ethanol and re-pelleted for 10 minutes twice. The ethanol was then aspirate and the DNA pellet allowed to dry at RT before resuspended in water and quantifying the DNA concentration via nanodrop. For each condition, 3 50μL PCR reactions were run each containing 0.7μL Phusion polymerase (NEB, M0530L), 1X Phusion HF buffer, 2M betaine, 1.5% DMSO, 400μM dNTPs, 40nM multiplexed genomic primers, 1μg genomic DNA. The reaction was then carried out as follows: 98°C 5mins, 12 cycles of 98°C 60s, 62°C 120s, 72°C 120s, then 72°C 5mins. All 3 reactions were combined and 100μL of the total reaction volume was taken forward for size selection. 60μL of SPRISelect beads (Beckman, B23318) was added to the 100μL PCR reaction mixture, mixed by pipetting and then incubated for 5mins at RT. The beads were collected on a magnetic rack and the supernatant was transferred to a fresh tube. 70μL of beads were then added to the supernatant and mixed by pipetting and incubated for 5mins at RT, binding the amplicons. Beads were then collected on the magnet, the supernatant was discarded and the beads were washed twice in 85% ethanol for 30s. The beads were then dried until most ethanol had evaporated, but the beads were still wet, and the beads were then resuspended in 50μL 10mM Tris pH 8.0 and incubated for 10 minutes at 37C with 1000rpm agitation. The 50uL of eluted amplicons were then transferred to a fresh tube and the process of bead selected was repeated but with half the volumes (30μL and 35μL of beads) and then eluted in 25μL of 10mM Tris pH 8.0. 10μL of these amplicons were then used for final library preparation using the NEB Ultra II DNA library prep kit according to manufacturer instructions using a 1/25 adapter dilution and 5 PCR cycles.

text: osteosarcoma

characteristic: disease

text: AID-AsiSI-ER expression

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: bone

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

text: University of Cambridge, The Gurdon Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: osteoblast

text: human

text: osteosarcoma

text: AID-AsiSI-ER expression

text: Homo sapiens

text: Homo sapiens

text: bone

text: Homo sapiens


text: public

tag: Namespace:Zurich University of Applied Sciences (ZHAW), School of Life Sciences and Facility Management, Institute of Natural Resource Sciences

text: V. Steffen

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Canton of Zurich, Volketswil

characteristic: geographic location (region and locality)

characteristic: isolate

text: grassland soil

text: 47.392111°N 8.656389°E

text: Streptomyces albidoflavus

characteristic: organism

text: Streptomyces albidoflavus

text: CCOS 2040

characteristic: strain

text: Streptomyces albidoflavus CCOS 2040


E-MTAB-11262:Sample 1

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: E-MTAB-11262:Sample 1

tag: Namespace:Division of Immunity, Inflammation and Infection John Curtin School of Medical Research The Australian National University

unit: day

characteristic: age

text: ArrayExpress

text: B-1a B cell

text: house mouse

text: Protocols: B1a B cells (CD19+B220-CD5+) were sorted from the spleen of IRF4-T95R heterozygous mice and wild-type littermates. RNA was isolated using the GenElute Single cell RNA purification kit (RNB300) from Sigma-Aldrich. 200 000 B1a Cells were sorted directly into 400µl of lysis buffer and 400µl of Ethanol were added. Further processing followed the protocol and included the on-column DNA removal step. Library prep was done using the NEB mRNA Ultra II kit according to manufacturers instructions

characteristic: description

text: adult

characteristic: developmental stage

characteristic: immunophenotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: male

characteristic: sex

text: Sample 1

characteristic: title


E-MTAB-11262:Sample 2

characteristic: ENA-CHECKLIST

text: Division of Immunity, Inflammation and Infection John Curtin School of Medical Research The Australian National University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11262:Sample 2

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: B-1a B cell

text: house mouse

characteristic: common name

text: adult

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: male

text: Sample 2


E-MTAB-11262:Sample 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

text: public

text: E-MTAB-11262:Sample 3

characteristic: age

text: ArrayExpress

text: B-1a B cell

text: house mouse

text: adult

text: wild type genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: male

text: Sample 3


E-MTAB-11262:Sample 4

text: public

text: E-MTAB-11262:Sample 4

text: ArrayExpress

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text: adult

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text: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System; Primers were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/). Primer targeting the V4F for the 18S rRNA gene (Stoeck et al. 2010)

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text: mimarks-survey

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text: mimarks-survey

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text: marine metagenome

text: Illumina MiSeq

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text: GFBIO

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text: coastal sea water [ENVO:00002150]

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text: water

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text: Svalbard

characteristic: geographic location (country and/or sea)

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text: mimarks-survey

text: marine metagenome

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characteristic: title

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characteristic: ENA-CHECKLIST

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text: 58203:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: water

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characteristic: geographic location (latitude)

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text: mimarks-survey

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text: marine metagenome

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text: GFBIO

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text: coastal sea water [ENVO:00002150]

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text: water

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text: mimarks-survey

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text: true

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text: marine metagenome

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text: true

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text: public

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text: 58198:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: GFBIO

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text: marine biome [ENVO:00000447] | polar biome [ENVO:01000339]

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text: coastal sea water [ENVO:00002150]

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text: water

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text: Svalbard

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text: mimarks-survey

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text: Illumina MiSeq

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text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

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text: 58197:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: water

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characteristic: geographic location (latitude)

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text: mimarks-survey

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text: marine metagenome

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text: GFBIO

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text: microbial feature [ENVO:01000007] | marine photic zone [ENVO:00000209]

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text: coastal sea water [ENVO:00002150]

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text: water

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text: public

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text: 58195:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: Svalbard

characteristic: geographic location (country and/or sea)

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text: mimarks-survey

text: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System; Primers were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/). Primer targeting the V4F for the 18S rRNA gene (Stoeck et al. 2010)

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text: marine metagenome

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text: coastal sea water [ENVO:00002150]

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text: mimarks-survey

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characteristic: geographic location (latitude)

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text: marine metagenome

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text: GFBIO

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characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

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text: true

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text: water

text: Svalbard

characteristic: geographic location (latitude)

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text: mimarks-survey

characteristic: investigation type

text: marine metagenome

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text: marine metagenome

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text: true

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text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

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characteristic: SRA accession

text: 58184:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: GFBIO

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text: water

characteristic: environmental package

text: Svalbard

characteristic: geographic location (country and/or sea)

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text: mimarks-survey

text: marine metagenome

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text: true

characteristic: title

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text: public

characteristic: SRA accession

text: 58182:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: GFBIO

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text: coastal sea water [ENVO:00002150]

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characteristic: investigation type

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text: marine metagenome

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text: true

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text: 58181:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: 58180:fffbcc8f-8982-4de4-a7e4-0b58a3b07f20

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text: GFBIO

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characteristic: geographic location (latitude)

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text: marine metagenome

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text: coastal sea water [ENVO:00002150]

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characteristic: project name

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