characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak

text: AND14409, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

tag: Namespace:Clinical Bioinformatics Area

text: Servicio de Microbiologia, Hospital Universitario San Cecilio, Granada, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

characteristic: description

text: Spain

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14411, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Complejo Hospitalario de Jaen, Jaen, Spain

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14412, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Virgen de la Victoria, Malaga, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14413, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14414, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14416, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14417, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14418, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14425, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14429, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14430, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14431, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak

text: AND14432, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14433, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14434, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Complejo Hospitalario de Jaen, Jaen, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14435, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14437, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14438, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14439, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14440, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14441, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14442, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: sample capture status

text: AND14443, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collection date

text: not provided

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: not provided

text: not provided

characteristic: isolate

characteristic: organism

text: AND14444, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

characteristic: description

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14445, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647202 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Eubacterium sp.

text: Autism spectrum disorder (ASD) is one of the most severe developmental disorders, affecting on average 1 in 150 children worldwide. There is a great need for more effective strategies to improve quality of life in ASD subjects. The gut microbiome has emerged as a potential therapeutic target in ASD. A novel modulator of the gut microbiome, the traditionally fermented milk drink kefir, has recently been shown to modulate the microbiota and decrease repetitive behaviour, one of the hallmarks of ASD, in mice. As such, we hypothesized that kefir could ameliorate behavioural deficits in a mouse model relevant to ASD; the BTBR T+ Itpr3tf/J mouse strain. To this end, adult mice were administered either kefir (UK4) or a milk control for three weeks as treatment lead-in, after which they were assessed for their behavioural phenotype using a battery of tests. In addition, we assessed systemic immunity by flow cytometry and the gut microbiome using shotgun metagenomic sequencing. We found that indeed kefir decreased repetitive behaviour in this mouse model. Furthermore, kefir prolonged stress-induced increases in corticosterone 60 min post-stress, which was accompanied by an ameliorated innate immune response as measured by LY6Chi monocyte levels. In addition, kefir increased the levels of anti-inflammatory Treg cells in mesenteric lymph nodes (MLNs). Kefir also increased the relative abundance of Lachnospiraceae bacterium A2, which correlated with reduced repetitive behaviour and increased Treg cells in MLNs. Functionally, kefir modulated various predicted gut microbial pathways, including the gut-brain module S-Adenosylmethionine (SAM) synthesis, as well as L-valine biosynthesis and pyruvate fermentation to isobutanol, which all correlated with repetitive behaviour. Taken together our data show that kefir modulates peripheral immunoregulation, can ameliorate specific ASD behavioural dysfunctions and modulates selective aspects of the composition and function of the gut microbiome, indicating that kefir supplementation might prove a viable strategy in improving quality of life in ASD subjects.

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647202_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647204 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647204_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Eubacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647204_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Prevotella sp.

characteristic: organism

text: uncultured Prevotella sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Prevotellaceae;g__Prevotella;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647204_bin.6_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647204_bin.7_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647205 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647205_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647206 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647206_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Hungatella sp.

characteristic: organism

text: uncultured Hungatella sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647206_bin.2_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647207 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

text: Autism spectrum disorder (ASD) is one of the most severe developmental disorders, affecting on average 1 in 150 children worldwide. There is a great need for more effective strategies to improve quality of life in ASD subjects. The gut microbiome has emerged as a potential therapeutic target in ASD. A novel modulator of the gut microbiome, the traditionally fermented milk drink kefir, has recently been shown to modulate the microbiota and decrease repetitive behaviour, one of the hallmarks of ASD, in mice. As such, we hypothesized that kefir could ameliorate behavioural deficits in a mouse model relevant to ASD; the BTBR T+ Itpr3tf/J mouse strain. To this end, adult mice were administered either kefir (UK4) or a milk control for three weeks as treatment lead-in, after which they were assessed for their behavioural phenotype using a battery of tests. In addition, we assessed systemic immunity by flow cytometry and the gut microbiome using shotgun metagenomic sequencing. We found that indeed kefir decreased repetitive behaviour in this mouse model. Furthermore, kefir prolonged stress-induced increases in corticosterone 60 min post-stress, which was accompanied by an ameliorated innate immune response as measured by LY6Chi monocyte levels. In addition, kefir increased the levels of anti-inflammatory Treg cells in mesenteric lymph nodes (MLNs). Kefir also increased the relative abundance of Lachnospiraceae bacterium A2, which correlated with reduced repetitive behaviour and increased Treg cells in MLNs. Functionally, kefir modulated various predicted gut microbial pathways, including the gut-brain module S-Adenosylmethionine (SAM) synthesis, as well as L-valine biosynthesis and pyruvate fermentation to isobutanol, which all correlated with repetitive behaviour. Taken together our data show that kefir modulates peripheral immunoregulation, can ameliorate specific ASD behavioural dysfunctions and modulates selective aspects of the composition and function of the gut microbiome, indicating that kefir supplementation might prove a viable strategy in improving quality of life in ASD subjects.

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647207_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647208 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647208_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647208_bin.3_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Eubacterium sp.

characteristic: organism

text: uncultured Eubacterium sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647208_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647210 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647210_bin.3_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647211 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647211_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647212 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647212_bin.2_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Autism spectrum disorder (ASD) is one of the most severe developmental disorders, affecting on average 1 in 150 children worldwide. There is a great need for more effective strategies to improve quality of life in ASD subjects. The gut microbiome has emerged as a potential therapeutic target in ASD. A novel modulator of the gut microbiome, the traditionally fermented milk drink kefir, has recently been shown to modulate the microbiota and decrease repetitive behaviour, one of the hallmarks of ASD, in mice. As such, we hypothesized that kefir could ameliorate behavioural deficits in a mouse model relevant to ASD; the BTBR T+ Itpr3tf/J mouse strain. To this end, adult mice were administered either kefir (UK4) or a milk control for three weeks as treatment lead-in, after which they were assessed for their behavioural phenotype using a battery of tests. In addition, we assessed systemic immunity by flow cytometry and the gut microbiome using shotgun metagenomic sequencing. We found that indeed kefir decreased repetitive behaviour in this mouse model. Furthermore, kefir prolonged stress-induced increases in corticosterone 60 min post-stress, which was accompanied by an ameliorated innate immune response as measured by LY6Chi monocyte levels. In addition, kefir increased the levels of anti-inflammatory Treg cells in mesenteric lymph nodes (MLNs). Kefir also increased the relative abundance of Lachnospiraceae bacterium A2, which correlated with reduced repetitive behaviour and increased Treg cells in MLNs. Functionally, kefir modulated various predicted gut microbial pathways, including the gut-brain module S-Adenosylmethionine (SAM) synthesis, as well as L-valine biosynthesis and pyruvate fermentation to isobutanol, which all correlated with repetitive behaviour. Taken together our data show that kefir modulates peripheral immunoregulation, can ameliorate specific ASD behavioural dysfunctions and modulates selective aspects of the composition and function of the gut microbiome, indicating that kefir supplementation might prove a viable strategy in improving quality of life in ASD subjects.

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647212_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647213 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647213_bin.2_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Akkermansia sp.

characteristic: organism

text: uncultured Akkermansia sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Verrucomicrobia;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647213_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647214 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647214_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Eubacterium plexicaudatum

characteristic: organism

text: Eubacterium plexicaudatum

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__Eubacterium plexicaudatum

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647214_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647215 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647215_bin.2_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus murinus

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647215_bin.6_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647216 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647216_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: Autism spectrum disorder (ASD) is one of the most severe developmental disorders, affecting on average 1 in 150 children worldwide. There is a great need for more effective strategies to improve quality of life in ASD subjects. The gut microbiome has emerged as a potential therapeutic target in ASD. A novel modulator of the gut microbiome, the traditionally fermented milk drink kefir, has recently been shown to modulate the microbiota and decrease repetitive behaviour, one of the hallmarks of ASD, in mice. As such, we hypothesized that kefir could ameliorate behavioural deficits in a mouse model relevant to ASD; the BTBR T+ Itpr3tf/J mouse strain. To this end, adult mice were administered either kefir (UK4) or a milk control for three weeks as treatment lead-in, after which they were assessed for their behavioural phenotype using a battery of tests. In addition, we assessed systemic immunity by flow cytometry and the gut microbiome using shotgun metagenomic sequencing. We found that indeed kefir decreased repetitive behaviour in this mouse model. Furthermore, kefir prolonged stress-induced increases in corticosterone 60 min post-stress, which was accompanied by an ameliorated innate immune response as measured by LY6Chi monocyte levels. In addition, kefir increased the levels of anti-inflammatory Treg cells in mesenteric lymph nodes (MLNs). Kefir also increased the relative abundance of Lachnospiraceae bacterium A2, which correlated with reduced repetitive behaviour and increased Treg cells in MLNs. Functionally, kefir modulated various predicted gut microbial pathways, including the gut-brain module S-Adenosylmethionine (SAM) synthesis, as well as L-valine biosynthesis and pyruvate fermentation to isobutanol, which all correlated with repetitive behaviour. Taken together our data show that kefir modulates peripheral immunoregulation, can ameliorate specific ASD behavioural dysfunctions and modulates selective aspects of the composition and function of the gut microbiome, indicating that kefir supplementation might prove a viable strategy in improving quality of life in ASD subjects.

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647216_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus reuteri

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647216_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647218 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647218_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647218_bin.2_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Parabacteroides goldsteinii

characteristic: organism

text: Parabacteroides goldsteinii

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides;s__Parabacteroides goldsteinii

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647218_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647218_bin.4_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647220 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: NextSeq 500

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647220_bin.4_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647221 of study ERP128045.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Eubacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647221_bin.3_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR5647223 of study ERP128045.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Pseudomonas aeruginosa

characteristic: organism

text: Autism spectrum disorder (ASD) is one of the most severe developmental disorders, affecting on average 1 in 150 children worldwide. There is a great need for more effective strategies to improve quality of life in ASD subjects. The gut microbiome has emerged as a potential therapeutic target in ASD. A novel modulator of the gut microbiome, the traditionally fermented milk drink kefir, has recently been shown to modulate the microbiota and decrease repetitive behaviour, one of the hallmarks of ASD, in mice. As such, we hypothesized that kefir could ameliorate behavioural deficits in a mouse model relevant to ASD; the BTBR T+ Itpr3tf/J mouse strain. To this end, adult mice were administered either kefir (UK4) or a milk control for three weeks as treatment lead-in, after which they were assessed for their behavioural phenotype using a battery of tests. In addition, we assessed systemic immunity by flow cytometry and the gut microbiome using shotgun metagenomic sequencing. We found that indeed kefir decreased repetitive behaviour in this mouse model. Furthermore, kefir prolonged stress-induced increases in corticosterone 60 min post-stress, which was accompanied by an ameliorated innate immune response as measured by LY6Chi monocyte levels. In addition, kefir increased the levels of anti-inflammatory Treg cells in mesenteric lymph nodes (MLNs). Kefir also increased the relative abundance of Lachnospiraceae bacterium A2, which correlated with reduced repetitive behaviour and increased Treg cells in MLNs. Functionally, kefir modulated various predicted gut microbial pathways, including the gut-brain module S-Adenosylmethionine (SAM) synthesis, as well as L-valine biosynthesis and pyruvate fermentation to isobutanol, which all correlated with repetitive behaviour. Taken together our data show that kefir modulates peripheral immunoregulation, can ameliorate specific ASD behavioural dysfunctions and modulates selective aspects of the composition and function of the gut microbiome, indicating that kefir supplementation might prove a viable strategy in improving quality of life in ASD subjects.

text: Pseudomonas aeruginosa

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas;s__Pseudomonas aeruginosa

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647223_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Escherichia coli

characteristic: project name

characteristic: sample derived from

text: Escherichia coli

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647223_bin.2_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus fermentum

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647223_bin.4_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Staphylococcus aureus

characteristic: project name

characteristic: sample derived from

text: Staphylococcus aureus

text: NextSeq 500

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Staphylococcaceae;g__Staphylococcus;s__Staphylococcus aureus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: ERR5647223_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Bacillus subtilis

characteristic: organism

text: Bacillus subtilis

text: NextSeq 500

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis

text: multi-marker approach

text: Metagenome-assembled genome: ERR5647223_bin.6_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Virgen de la Victoria, Malaga, Spain

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: AND14446, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Complejo Hospitalario de Jaen, Jaen, Spain

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14447, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

text: ena-upload-cli

characteristic: collecting institution

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14448, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14449, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14450, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14451, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14452, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14453, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14454, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14456, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14457, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14458, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14459, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: Servicio de Microbiologia, Hospital Virgen de la Victoria, Malaga, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14460, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: active surveillance in response to outbreak

text: AND14461, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14462, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14463, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14464, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio Andaluz de Salud, Andalusia, Spain

text: not provided

text: Spain

text: not provided

text: not provided

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14465, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14466, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14467, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14468, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14469, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND14470, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND14471, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND14472, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region