text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

characteristic: common name

text: saliva material

characteristic: environmental medium

text: Tanzania

text: oral cavity

text: uncultured bacterium

characteristic: organism

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C46_D3_p

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:STATENS SERUM INSTITUT

characteristic: Submitter Id

text: human

characteristic: broad-scale environmental context

characteristic: collection date

text: saliva material

text: Tanzania

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: oral cavity

characteristic: local environmental context

text: uncultured bacterium

text: RARE

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: Human oral cavity sample (Saliva) - C47_D3_p


text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

characteristic: common name

text: saliva material

characteristic: environmental medium

text: Tanzania

text: oral cavity

text: uncultured bacterium

characteristic: organism

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C48_D3_p

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: saliva material

text: Tanzania

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C49_D3_p


text: STATENS SERUM INSTITUT

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

characteristic: broad-scale environmental context

characteristic: collection date

text: saliva material

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C50_D3_p


text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

text: saliva material

text: Tanzania

characteristic: geographic location (longitude)

text: oral cavity

characteristic: local environmental context

text: uncultured bacterium

text: RARE

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: Human oral cavity sample (Saliva) - C51_D3_p


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: STATENS SERUM INSTITUT

text: public

text: human

characteristic: common name

text: saliva material

characteristic: environmental medium

text: Tanzania

text: oral cavity

text: uncultured bacterium

characteristic: organism

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C52_D3_p

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: saliva material

text: Tanzania

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C53_D3_p


text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: human

characteristic: broad-scale environmental context

characteristic: collection date

text: saliva material

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Illumina MiSeq

text: Human oral cavity sample (Saliva) - C54_D3_p


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: STATENS SERUM INSTITUT

text: public

text: human

text: saliva material

text: Tanzania

characteristic: geographic location (longitude)

text: oral cavity

characteristic: local environmental context

text: uncultured bacterium

text: RARE

characteristic: project name

text: uncultured bacterium

text: Illumina MiSeq

characteristic: sequencing method

text: Human oral cavity sample (Saliva) - C55_D3_p


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

text: public

text: human

characteristic: common name

text: saliva material

characteristic: environmental medium

text: Tanzania

text: oral cavity

text: uncultured bacterium

characteristic: organism

text: RARE

text: uncultured bacterium

text: Oxford Nanopore MinION

text: Human oral cavity sample (Saliva) - P_d0_bact

characteristic: title


text: STATENS SERUM INSTITUT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: saliva material

text: Tanzania

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Oxford Nanopore MinION

text: Human oral cavity sample (Saliva) - P_d0_phage


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: STATENS SERUM INSTITUT

text: public

text: human

characteristic: broad-scale environmental context

characteristic: collection date

text: saliva material

text: Tanzania

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: oral cavity

text: uncultured bacterium

text: RARE

text: uncultured bacterium

text: Oxford Nanopore MinION

text: Human oral cavity sample (Saliva) - P_d3_bact


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: STATENS SERUM INSTITUT

text: public

text: human

text: saliva material

text: Tanzania

characteristic: geographic location (longitude)

text: oral cavity

characteristic: local environmental context

text: uncultured bacterium

text: RARE

characteristic: project name

text: uncultured bacterium

text: Oxford Nanopore MinION

characteristic: sequencing method

text: Human oral cavity sample (Saliva) - P_d3_phage


ECOARCOLBOV

text: UNIVERSIDAD DE JAEN

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ECOARCOLBOV

tag: Namespace:UNIVERSIDAD DE JAEN

text: Antibiotic-resistant E. coli strain isolated from bovine intestine in Colombia

characteristic: description

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: ECOARCOLBOV

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Liverpool

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Liverpool

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: Frozen control knee articular cartilage tissue was obtained from xxx. Osteoarthritic articular cartilage was dissected from osteochondral tissue obtained following total knee arthroplasty and snap frozen in liquid nitrogen. Frozen tissues were powderised in a dismembrenator and then nucleic acids extracted using Trizol. Chloroform extraction was performed to enrich total RNA and then these extracts were processed using a Qiagen RNeasy kit. QuantSeq-Reverse

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: cartilage

characteristic: organism part

text: knee

characteristic: sampling site

text: Homo sapiens

text: male

characteristic: sex


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

characteristic: description

text: adult

text: osteoarthritis, knee

text: Homo sapiens

text: Homo sapiens

text: cartilage

text: knee

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

text: Shanghai Tenth's People's Hospital

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Shanghai Tenth's People's Hospital

text: ArrayExpress

text: Protocols: Cells were grown in YPD broth at 37˚C from OD600=0.01 till OD600=1.0. Cells were treated with 1 µg/ml or 4 µg/ml of tunicamycin, or DMSO (control) for 1h. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

text: wild type genotype

characteristic: genotype

text: Candida albicans

characteristic: organism

text: Candida albicans

text: whole organism

text: Candida albicans

characteristic: strain

characteristic: title


text: Shanghai Tenth's People's Hospital

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: aneuploid

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Insituto de Agrobiotecnologia del Litoral

text: thale cress

characteristic: common name

text: Capture-C of Ba-1 rosette leaves of 21-day-old plants, replicate 1

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: rosette leaves

text: Capture-C of Ba-1 rosette leaves, replicate 1


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Insituto de Agrobiotecnologia del Litoral

text: public

text: thale cress

text: Capture-C of Ba-1 rosette leaves of 21-day-old plants, replicate 2

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: rosette leaves

text: Capture-C of Ba-1 rosette leaves, replicate 2


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: thale cress

text: Capture-C of Col-0 rosette leaves of 21-day-old plants, replicate 1

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: rosette leaves

text: Capture-C of Col-0 rosette leaves, replicate 1


characteristic: ENA-CHECKLIST

text: public

text: thale cress

text: Capture-C of Col-0 rosette leaves of 21-day-old plants, replicate 2

characteristic: description

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: rosette leaves

text: Capture-C of Col-0 rosette leaves, replicate 2


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: thale cress

text: Capture-C of dcl234 rosette leaves of 21-day-old plants, replicate 1

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: dcl2, dcl3, dcl4 mutant

text: rosette leaves

text: Capture-C of dcl234 rosette leaves, replicate 1


text: public

characteristic: SRA accession

text: thale cress

text: Capture-C of dcl234 rosette leaves of 21-day-old plants, replicate 2

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: dcl2, dcl3, dcl4 mutant

text: rosette leaves

text: Capture-C of dcl234 rosette leaves, replicate 2


text: public

text: thale cress

text: Capture-C of ddc rosette leaves of 21-day-old plants, replicate 1

text: 21-day-old plants

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana

text: drm1, drm2, cmt3 mutant

text: rosette leaves

text: Capture-C of ddc rosette leaves, replicate 1


text: public

text: thale cress

text: Capture-C of ddc rosette leaves of 21-day-old plants, replicate 2

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: drm1, drm2, cmt3 mutant

characteristic: strain

text: rosette leaves

text: Capture-C of ddc rosette leaves, replicate 2


text: public

text: thale cress

text: Capture-C of Hod rosette leaves of 21-day-old plants, replicate 1

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Hod

text: rosette leaves

text: Capture-C of Hod rosette leaves, replicate 1

characteristic: title


text: Insituto de Agrobiotecnologia del Litoral

text: public

characteristic: Submitter Id

text: thale cress

text: Capture-C of Hod rosette leaves of 21-day-old plants, replicate 2

text: 21-day-old plants

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Hod

text: rosette leaves

text: Capture-C of Hod rosette leaves, replicate 2


text: University Hospital Basel

text: public

tag: Namespace:University Hospital Basel

text: Switzerland

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: human

characteristic: host scientific name

characteristic: isolate

text: rectal swab

text: Proteus mirabilis

text: Proteus mirabilis

text: ESBL LTCF study


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University Hospital Basel

text: public

text: Switzerland

text: not collected

text: human

text: rectal swab

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: ESBL LTCF study


characteristic: External Id

text: University Hospital Basel

characteristic: INSDC center name

text: public

text: Switzerland

text: not collected

text: human

text: rectal swab

text: Citrobacter farmeri

text: Citrobacter farmeri

text: ESBL LTCF study


characteristic: ENA-CHECKLIST

text: University Hospital Basel

characteristic: INSDC first public

text: public

text: Switzerland

text: not collected

text: human

text: rectal swab

text: Escherichia coli

text: Escherichia coli

text: ESBL LTCF study


text: University Hospital Basel

characteristic: INSDC last update

text: public

text: Switzerland

text: not collected

text: human

text: rectal swab

text: Klebsiella oxytoca

text: Klebsiella oxytoca

text: ESBL LTCF study


text: Lancaster University

text: public

characteristic: INSDC status

tag: Namespace:Lancaster University

text: Andrew Prestt

text: Suppressive field bulk soil

characteristic: description

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field 16S rep 1


text: Lancaster University

text: public

characteristic: SRA accession

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

characteristic: organism

text: soil metagenome

text: Supp field 16S rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field 16S rep 3

characteristic: title


text: Lancaster University

text: public

characteristic: Submitter Id

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field 16S rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field 16S rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field 16S rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field 16S rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field 16S rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot 16S rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot 16S rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot 16S rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot 16S rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot 16S rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot 16S rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot 16S rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot 16S rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot 16S rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot 16S rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot 16S rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot 16S rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field ITS2 rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field ITS2 rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field ITS2 rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Suppressive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Supp field ITS2 rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field ITS2 rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field ITS2 rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field ITS2 rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Conducive field bulk soil

text: Yes

text: Bulk field soil, no plants present

text: soil metagenome

text: soil metagenome

text: Cond field ITS2 rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot ITS2 rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot ITS2 rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot ITS2 rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, suppressive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Supp Pot ITS2 rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot ITS2 rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot ITS2 rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot ITS2 rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, conducive soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Cond pot ITS2 rep 4


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot ITS2 rep 1


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot ITS2 rep 2


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot ITS2 rep 3


text: Lancaster University

text: public

text: Andrew Prestt

text: Pot experiment, mixed soil

text: Yes

text: Pot experiment, onion plants present

text: soil metagenome

text: soil metagenome

text: Mix pot ITS2 rep 4


text: male

characteristic: ArrayExpress-SEX

text: Streptococcus pneumoniae

characteristic: ArrayExpress-SPECIES

text: Male

characteristic: GENDER

text: SC

text: public

text: Streptococcus pneumoniae

characteristic: common name

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: female

text: Streptococcus pneumoniae

characteristic: ENA-FIRST-PUBLIC

text: Female

text: SC

text: public

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


characteristic: ENA-LAST-UPDATE

text: University of Salerno

text: public

tag: Namespace:University of Salerno

text: ArrayExpress

characteristic: broker name

text: Protocols: C. albicans cultures were cross-linked with 1% formaldehyde for 15 minutes at room temperature with gentle shaking. The reaction was quenched with 125 mM glycine and cells wer havested by centrifugation. Budding yeast cell culture was diluted at a cell density of 10^5 cells/mL in YPD and incubated at 25°C for 28 h with or without 10 mM NAM. ChIP was performed as previusly described (Mawer, J., Massen, J., Reichert, C., Grabenhorst, N., Mylonas, C., and Tessarz, P. (2021). Nhp2 is areader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.EMBO reports, 22(10), e52435. doi: 10.15252/embr.202152435) except for cell lysis that was carried out by using a cryogenic freezer mill (SPEX SamplePrep 6970EFM Freezer/Mill, München, Germany). DNA from Input and ChIP samples was purified with Macherey-Nagel NucleoSpin Gel and PCR Clean-Up Kit. Libraries were generated from H3K56ac and input following a previously published protocol (Ford, E., Nikopoulou, C., Kokkalis, A., and Thanos, D. (2014). A method for generating highly multiplexed ChIP-seq libraries. BMC research notes, 7, 312. doi: 10.1186/1756-0500-7-312).

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:Institute of Molecular Genetics and Genetic Engineering

text: Norway rat

text: DA control

text: Rattus norvegicus

text: Rattus norvegicus

text: male

characteristic: sex

text: Dark Agouti

characteristic: strain

text: duodenum


text: Institute of Molecular Genetics and Genetic Engineering

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Norway rat

text: DA control

characteristic: description

text: Rattus norvegicus

text: Rattus norvegicus

text: male

text: Dark Agouti

text: duodenum


text: public

characteristic: Submitter Id

text: Norway rat

text: DA cadmium 5 ppm

text: Rattus norvegicus

characteristic: organism

text: Rattus norvegicus

text: male

text: Dark Agouti

text: duodenum

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: public

text: Norway rat

characteristic: common name

text: DA cadmium 50 ppm

text: Rattus norvegicus

text: Rattus norvegicus

text: male

text: Dark Agouti

text: duodenum


characteristic: ENA-LAST-UPDATE

text: public

text: Norway rat

text: DA cadmium 5 ppm EPS-AN8

text: Rattus norvegicus

text: Rattus norvegicus

text: male

text: Dark Agouti

text: duodenum


text: public

text: Norway rat

text: DA cadmium 50 ppm EPS-AN8

text: Rattus norvegicus

text: Rattus norvegicus

text: male

text: Dark Agouti

text: duodenum


text: public

tag: Namespace:Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T helper 9 cell

text: human

text: Protocols: Peripheral Blood Mononuclear Cells (PBMC) were isolated by Ficoll-Plaque Plus (GE Healthcare, UK) density gradient centrifugation. Human CD4+ T cells were isolated from PBMC by EasySep positive selection kit (Stemcell Technologies) according to manufacturer’s instruction. Positively selected CD4+ T cells were washed with PBS and stained for subsequent Th cell subset sorting. Effector memory Th9 cells (CXCR3-CCR8+CCR6-CCR4+) were sorted to over 90% purity according to their expression of chemokine receptors from CD45RA-CD25-CD8-CD3+ cells. Single cell Th9 clones were directly sorted into 96well plate according to their expression of chemokine receptors. Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 50 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96 well plate in a total volume of 200μL cell culture medium Th9 cell clones were incubated with GW9662 for 48 hours and activated with αCD3/2/28 for 12 hours Total RNA was isolated with RNAeasy kit (Qiagen) Illumina TruSeq Stranded mRNA protocol (Directional, first strand)

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

characteristic: replicate

text: Homo sapiens

text: male

text: antibodies against CD3, CD2, and CD28 (12 hours)

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: T helper 9 cell

text: human

text: adult

text: normal

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

characteristic: sex


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: T helper 1 cell

text: human

text: Protocols: Peripheral Blood Mononuclear Cells (PBMC) were isolated by Ficoll-Plaque Plus (GE Healthcare, UK) density gradient centrifugation. Human naïve CD4+ T Cell Isolation Kit Human (Stemcell Technologies) as per the manufacturer’s instructions. Naïve T cells were stimulated with anti-CD3/CD2/CD28 beads (T cell/bead = 2:1, Miltenyi) and primed into effector CD4+ T cell subsets with IL-12 (5 ng/ml) (BioLegend) for TH1 cells, IL-4 (50 ng/ml) (BioLegend) for TH2 cells, IL-4 (50 ng/ml) and TGF-b (5 ng/ml) (R&D Systems) for TH9 cells, and TGF-b (5 ng/ml) for iTREG. From cell culture initiation to analysis, the culture medium was supplemented with the indicated cytokines every other day. Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 50 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96 well plate in a total volume of 200μL cell culture medium Naïve T cells were stimulated with anti-CD3/CD2/CD28 beads (T cell/bead = 2:1, Miltenyi) and primed into effector CD4+ T cell subsets with IL-12 (5 ng/ml) (BioLegend) for TH1 cells, IL-4 (50 ng/ml) (BioLegend) for TH2 cells, IL-4 (50 ng/ml) and TGF-b (5 ng/ml) (R&D Systems) for TH9 cells, and TGF-b (5 ng/ml) for iTREG. Total RNA was isolated with RNAeasy kit (Qiagen) Illumina TruSeq Stranded mRNA protocol (Directional, first strand)

characteristic: description

text: adult

text: normal

characteristic: growth condition

text: A

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

text: antibodies against CD3, CD2, and CD28 (7 days)

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T helper 2 cell

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

text: A

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: T helper 9 cell

text: human

text: adult

text: normal

text: IL-4 and TGF-b

text: A

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

characteristic: sex

characteristic: stimulus


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: regulatory T cell

text: human

characteristic: description

text: adult

text: normal

text: TGF-b

characteristic: growth condition

text: A

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: male


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T helper 1 cell

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: B

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: T helper 2 cell

text: human

text: adult

text: normal

characteristic: disease

text: B

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: T helper 9 cell

text: human

characteristic: description

text: adult

text: normal

text: IL-4 and TGF-b

characteristic: growth condition

text: B

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: male

characteristic: sex

characteristic: stimulus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: regulatory T cell

text: human

characteristic: common name

text: adult

text: normal

text: TGF-b

text: B

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: T helper 1 cell

text: human

text: adult

characteristic: developmental stage

text: normal

text: C

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: T helper 2 cell

text: human

characteristic: description

text: adult

text: normal

characteristic: disease

characteristic: growth condition

text: C

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: female


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: T helper 9 cell

text: human

characteristic: common name

text: adult

text: normal

text: IL-4 and TGF-b

text: C

text: Homo sapiens

text: Homo sapiens

text: blood

text: Homo sapiens

text: female

characteristic: sex

characteristic: stimulus


characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

text: regulatory T cell

text: human

text: adult

text: normal

text: TGF-b

text: C

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: Homo sapiens

text: female


text: Shanghai Tenth People's Hospital

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Shanghai Tenth People's Hospital

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Cells were grown in YPD broth at 37˚C from OD600=0.01 till OD600=1.0. Cells were treated with 1 µg/ml or 4 µg/ml of tunicamycin, or DMSO (control) for 1h. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. The pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA). Buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library.

characteristic: description

text: Candida albicans

text: Candida albicans

text: whole organism

text: Candida albicans

characteristic: strain

unit: Celsius

characteristic: temperature

characteristic: title


E-MTAB-12200:LR48_ B

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Shanghai Tenth People's Hospital

text: public

text: E-MTAB-12200:LR48_ B

text: ArrayExpress

characteristic: broker name

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: Candida albicans

text: LR48_ B


characteristic: ENA-CHECKLIST

characteristic: External Id

text: public

tag: Namespace:Institut de recherche experimentale et clinique - IREC

text: Isolate CTMA_1650 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: Institut de recherche experimentale et clinique - IREC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Isolate CTMA_1652 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


characteristic: INSDC last update

text: public

text: Isolate CTMA_1653 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1654 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae


text: public

characteristic: INSDC status

text: Isolate CTMA_1655 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

characteristic: SRA accession

text: Isolate CTMA_1656 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

characteristic: Submitter Id

text: Isolate CTMA_1657 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1659 of Klebsiella pneumoniae

characteristic: description

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1660 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1661 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1662 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1663 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1664 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1665 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1666 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1667 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1668 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: Institut de recherche experimentale et clinique - IREC

text: public

text: Isolate CTMA_1671 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1673 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1674 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1675 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1676 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1677 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1678 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1679 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1680 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1681 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1682 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: public

text: Isolate CTMA_1683 of Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


USA 300 WT

text: Norah

text: public

text: USA 300 WT

text: Norah alqahtani

characteristic: collection date

text: WGS of WT

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: Skin

characteristic: host scientific name

text: not collected

characteristic: isolate

text: Lab

text: Staphylococcus aureus subsp. aureus USA300

characteristic: serovar

characteristic: strain

text: Wild type


name: USA300(Competition between   USA300 and IVK83 ∆efiTP) colony 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Norah

text: public

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved

characteristic: title


name: USA300(Competition between   USA300 and IVK83 ∆efiTP) colony 2

characteristic: External Id

text: Norah

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 ∆efiTP) colony 3

text: Norah

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

characteristic: organism

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 ∆efiTP) colony 4

text: Norah

text: public

text: Norah alqahtani

characteristic: collection date

text: cometitive fitness whith S.epidermidis

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

characteristic: host health state

text: Skin

text: not collected

text: Lab

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 ∆efiTP) colony 5

text: Norah

text: public

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

characteristic: host scientific name

text: not collected

characteristic: isolate

text: Lab

characteristic: strain

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 WT) colony 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Norah

text: public

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 WT) colony 2

characteristic: External Id

text: Norah

characteristic: INSDC center name

text: public

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved

characteristic: title


name: USA300(Competition between   USA300 and IVK83 WT) colony 3

text: Norah

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 WT) colony 4

text: Norah

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Norah alqahtani

text: cometitive fitness whith S.epidermidis

text: United Kingdom

text: not collected

text: Skin

text: not collected

text: Lab

characteristic: organism

text: USA300 evolved


name: USA300(Competition between   USA300 and IVK83 WT) colony 5

text: Norah

text: public

text: Norah alqahtani

characteristic: collection date

text: cometitive fitness whith S.epidermidis

characteristic: description

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: not collected

text: Skin

text: not collected

text: Lab

text: USA300 evolved


text: Friedrich-Loeffler-Institute

text: public

text: Landeslabor Berlin-Brandenburg

characteristic: collecting institution

text: Hlinak

characteristic: collector name

text: African swine fever virus

characteristic: common name

text: Germany

text: Wild boar

characteristic: host common name

text: Death

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not collected

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: Bone marrow

characteristic: isolation source host-associated

text: African swine fever virus

text: African swine fever virus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Landesuntersuchungsamt Sachsen, Dresden

text: Muluneh

text: African swine fever virus

text: Germany

text: Wild boar

text: Death

text: diseased

text: Sus scrofa

text: not collected

text: Blood swab

text: African swine fever virus

text: African swine fever virus


text: Friedrich-Loeffler-Institute

text: public

characteristic: Submitter Id

text: Landesuntersuchungsamt Sachsen, Leipzig

characteristic: collecting institution

text: Konrath

characteristic: collector name

text: African swine fever virus

characteristic: common name

text: Germany

characteristic: geographic location (country and/or sea)

text: Wild boar

characteristic: host common name

text: Death

text: diseased

text: Sus scrofa

text: not collected

text: Spleen

text: African swine fever virus

characteristic: organism

text: African swine fever virus


text: Friedrich-Loeffler-Institute

text: public

text: Landeslabor Berlin-Brandenburg

text: Hlinak

text: African swine fever virus

text: Germany

text: Wild boar

text: Death

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not collected

characteristic: host sex

characteristic: host subject id

characteristic: isolate

text: Blood swab

characteristic: isolation source host-associated

text: African swine fever virus

text: African swine fever virus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Landesuntersuchungsamt Sachsen, Dresden

text: Muluneh

text: African swine fever virus

text: Germany

text: Wild boar

text: Death

text: diseased

text: Sus scrofa

text: not collected

text: EDTA-Blood

text: African swine fever virus

text: African swine fever virus


text: Friedrich-Loeffler-Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Landesuntersuchungsamt Sachsen, Dresden

characteristic: collecting institution

text: Muluneh

characteristic: collector name

text: African swine fever virus

characteristic: common name

text: Germany

characteristic: geographic location (country and/or sea)

text: Wild boar

text: Death

text: diseased

text: Sus scrofa

text: not collected

text: Bone marrow

text: African swine fever virus

text: African swine fever virus


text: Friedrich-Loeffler-Institute

text: public

text: Landeslabor Berlin-Brandenburg

text: Hlinak

text: African swine fever virus

text: Germany

text: Domestic pig

characteristic: host common name

text: Death

characteristic: host disease outcome

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not collected

characteristic: host sex

characteristic: host subject id

text: EDTA-Blood

text: African swine fever virus

characteristic: organism

text: African swine fever virus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NTNU

characteristic: INSDC center name

text: public

text: Production of salmon smolts

characteristic: broad-scale environmental context

characteristic: collection date

text: Biofilm surface tank from RAS

characteristic: description

text: Biofilm surface tank

characteristic: environmental medium

text: Norway

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: RAS facility

characteristic: local environmental context

text: biofilter metagenome

text: Monitoring of microbiota in RAS

characteristic: project name

text: biofilter metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


text: NTNU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Production of salmon smolts

text: Biofilm biofilter from RAS

text: Biofilter biofilm

text: Norway

characteristic: geographic location (country and/or sea)

text: RAS facility

text: biofilter metagenome

text: Monitoring of microbiota in RAS

text: biofilter metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NTNU

characteristic: INSDC center name

text: public

text: Production of salmon smolts

text: water rearing tank from RAS

text: water rearing tanks

text: Norway

text: RAS facility

text: freshwater metagenome

characteristic: organism

text: Monitoring of microbiota in RAS

text: freshwater metagenome

text: Illumina


text: NTNU

text: public

text: Production of salmon smolts

characteristic: broad-scale environmental context

characteristic: collection date

text: water in circulating loop RAS

characteristic: description

text: water in circulating loop

characteristic: environmental medium

text: Norway

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: RAS facility

text: freshwater metagenome

text: Monitoring of microbiota in RAS

text: freshwater metagenome

text: Illumina


text: NTNU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Production of salmon smolts

text: biofilter biofilm from RAS

text: Biofilter biofilm

text: Norway

text: RAS facility

characteristic: local environmental context

text: biofilter metagenome

text: Monitoring of microbiota in RAS

characteristic: project name

text: biofilter metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTNU

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Production of salmon smolts

text: rearing water from RAS

text: rearing water

text: Norway

characteristic: geographic location (country and/or sea)

text: RAS facility

text: freshwater metagenome

text: Monitoring of microbiota in RAS

text: freshwater metagenome

text: Illumina


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NTNU

characteristic: INSDC center name

text: public

text: Production of salmon smolts

characteristic: broad-scale environmental context

characteristic: collection date

text: water circulating loop RAS

text: water circulating loop

text: Norway

text: RAS facility

text: freshwater metagenome

characteristic: organism

text: Monitoring of microbiota in RAS

text: freshwater metagenome

text: Illumina


text: NTNU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Production of salmon smolts

text: biofilter biofilm from RAS

characteristic: description

text: biofilter bif

characteristic: environmental medium

text: Norway

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: RAS facility

text: biofilter metagenome

text: Monitoring of microbiota in RAS

text: biofilter metagenome

text: Illumina


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: NTNU

text: public

text: Production of salmon smolts

text: biofilter biofilm from RAS

text: biofilter biofilm

text: Norway

text: RAS facility

characteristic: local environmental context

text: biofilter metagenome

text: Monitoring of microbiota in RAS

characteristic: project name

text: biofilter metagenome

text: Illumina

characteristic: sequencing method

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSIDAD AUSTRAL DE CHILE

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSIDAD AUSTRAL DE CHILE

characteristic: Submitter Id

text: Snow

text: Antarctica

characteristic: geographic location (region and locality)

text: snow metagenome

text: snow metagenome


text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:ENS Lyon

text: Protoplasts isolated from maize leaf 2 weeks after sowing

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: Targeted mutagenesis of gene Zm00001e019823 (GeneID v5). Target sequence: ACCCGGAGGATGACTACGTCTGG. Forward primer: CGTACGTGCTCGCCAACAAG. Reverse primer: GGGCTGTGGAGGAAGAATC. Expected size: 160bp.

text: 48h after transfection

text: Transfection with CRISPR/Cas9 constructs

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

text: Protoplasts in W5 medium, at 26�C, in the dark

characteristic: growth condition

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target gene: ZmTPS7 (Target 1)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of gene Zm00001e019823 (GeneID v5). Target sequence: GTCGCTTCGACCGCAGCGCTTGG. Forward primer: CTTCTCATCGCCCGCTTCCG. Reverse primer: GATTACCTCCACGACCTTCTC. Expected size: 169bp.

characteristic: description

text: 48h after transfection

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmTPS7 (Target 2)

characteristic: title


text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: Targeted mutagenesis of gene Zm00001e008118 (GeneID v5). Target sequence: GACCAAGCTCGCAATCCAGGCGG. Forward primer: AGGCTCCCAAACCCCGCAG. Reverse primer: CGATCTGGTGAGCCTGGACG. Expected size: 183bp.

text: 48h after transfection

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

characteristic: growth condition

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmZOU (Target 2)


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

text: public

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of gene Zm00001e014975 (GeneID v5). Target sequences: GGATCCAATAACTCCAGTACAGG and GGTGTGCCAACAACTGAGGATGG. Forward primer: GAGGAGGAGAAGTCGTCTGC. Reverse primer: CAAGGAGCAGTCAGACGATG. Expected size: 202bp.

text: 48h after transfection

text: Maize inbred line A188

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target gene: ZmOSD1-1


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of gene Zm00001e014976 (GeneID v5). Target sequences: GCCTAGAGGAGGATGCGGCTCGG and CCAATCGAATGAAGATTCTTCAC. Forward primer: TAGTCCATGCTTTTGTTTG. Reverse primer: CTTTGAGAATCAGGATCAC. Expected size: 200bp.

characteristic: description

text: 48h after transfection

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmOSD1-2

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

characteristic: INSDC last update

text: public

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: Targeted mutagenesis of gene Zm00001e008508 (GeneID v5). Target sequence: ATGATGAAGATTCAGGGTTCGGG. Forward primer: AGATAAGGGTAAGGAGCAGCAG. Reverse primer: CGATGGCAGTATATCGTCCAGG. Expected size: 217bp.

text: 48h after transfection

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

characteristic: growth condition

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmKAK1


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

text: public

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Base editing of gene Zm00001e018755 (GeneID v5). Target sequence: GGTCAAGGAAGAACTTTGCCCGG. Forward primer: GGACCAACATCAGCTAGCTTCAACC. Reverse primer: GCAGGCTTAAGTAGCAATGTATCACACA . Expected size: 196bp.

text: 48h after transfection

text: Maize inbred line A188

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target gene: ZmICEa


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Base editing of gene Zm00001e018755 (GeneID v5). Target sequence: GGCTCATTCCCAAGCCCTACTGG. Forward primer: GGACCAACATCAGCTAGCTTCAACC. Reverse primer: GCAGGCTTAAGTAGCAATGTATCACACA. Expected size: 196bp.

characteristic: description

text: 48h after transfection

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmICEa

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

characteristic: INSDC last update

text: public

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: Base editing of gene Zm00001e008118 (GeneID v5). Target sequence: GAACAACGTGCCCGTGAGGCCGG. Forward primer: TGGGTCGTTCAAGGCTGCCAGA. Reverse primer: TGGTCCACATCCTCCACCGCG. Expected size: 159bp.

text: 48h after transfection

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

characteristic: growth condition

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmZOU


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

text: public

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of genes Zm00001e035023 and Zm00001e010407 (GeneID v5). Target sequence: CGAGTTCACCGGAGCAATCCCGG. Forward primer: SAGCCACAACCGCCTGTC. Reverse primer: GCACTGTTCCATTGATCTGG. Expected size: 173bp.

text: 48h after transfection

text: Maize inbred line A188

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target genes: ZmGSOa and ZmGSOb


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of genes Zm00001e035023 and Zm00001e010407 (GeneID v5). Target sequence: ATCACAACAAGCTCACCGGTCGG. Forward primer: TSCTCAACAACAACAGCCTCTC. Reverse primer: GRGAACTGGTTCTCGTACAGGT. Expected size: 166bp.

characteristic: description

text: 48h after transfection

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target genes: ZmGSOa and ZmGSOb

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

characteristic: INSDC last update

text: public

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: 48h after transfection

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

characteristic: growth condition

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target genes: : ZmGSOa and ZmGSOb


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

text: public

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of gene Zm00001e008508 (GeneID v5). Target sequence: GTGATGAAGATTCAGGGTTCGGG. Forward primer: AGATAAGGGTAAGGAGCAGCAG. Reverse primer: CGATGGCAGTATATCGTCCAGG. Expected size: 217bp.

text: 48h after transfection

text: Maize inbred line A188

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target gene: ZmKAK1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of gene Zm00001e008508 (GeneID v5). Target sequence: GATGATGAAGATTCAGGGTTCGGG. Forward primer: AGATAAGGGTAAGGAGCAGCAG. Reverse primer: CGATGGCAGTATATCGTCCAGG. Expected size: 217bp.

characteristic: description

text: 48h after transfection

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target gene: ZmKAK1

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

characteristic: INSDC last update

text: public

characteristic: age

text: Leaf protoplast

text: maize

characteristic: common name

text: Maize inbred line A188

characteristic: cultivar

text: Targeted mutagenesis of genes Zm00001e011125 and Zm00001e021494 (GeneID v5). Target sequences: ACTCCTCAACGTGGGCGTGTTCGG and GACGAAGACGCTGACGGAGAAGG. Forward primer: CAGCTGTTCACGGCCAAGAT. Reverse primer: GCGCTTCTGAAATTGAGGACTG. Expected size: 199bp.

text: 48h after transfection

characteristic: genetic modification

text: Maize inbred line A188

characteristic: genotype

characteristic: growth condition

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target genes: Sweet14a and Sweet14b


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: ENS Lyon

text: public

text: Protoplasts isolated from maize leaf 2 weeks after sowing

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of genes Zm00001e011125 and Zm00001e021494 (GeneID v5). Target sequences: ACTCCTCAACGTGGGCGTGTTCGG and GACGAAGACGCTGACGGAGAAGG. Forward primer: CAGCTGTTCACGGCCAAGAT. Reverse primer: ATATGCATGGTGTTGGGTAGTGTA. Expected size: 194bp.

text: 48h after transfection

text: Maize inbred line A188

characteristic: individual

text: Zea mays subsp. mays

characteristic: organism

text: Leaf

characteristic: organism part

text: Diploid

characteristic: ploidy

text: Fierlej et al.

characteristic: protocol

text: 48h after transfection

characteristic: sampling time point

text: Zea mays subsp. mays

text: Hermaphrodite

characteristic: sex

text: Target genes: Sweet14a and Sweet14b


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ENS Lyon

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Leaf protoplast

text: maize

text: Maize inbred line A188

text: Targeted mutagenesis of genes Zm00001e022582 and Zm00001e014642 (GeneID v5). Target sequences: GTACCTGGTGTACGCGCCCAAGG and AAGACGAAGCCCAGCACGTTGGG. Forward primer: GCGTCGTCGAGACCGTGTA. Reverse primer: GTCGGAGAGAAGCATGGTGAC. Expected size: 140bp.

characteristic: description

text: 48h after transfection

text: Transfection with CRISPR/Cas9 constructs

text: Maize inbred line A188

text: Zea mays subsp. mays

text: Leaf

text: Diploid

text: Fierlej et al.

text: 48h after transfection

text: Zea mays subsp. mays

text: Hermaphrodite

text: Target genes: Sweet15a and Sweet15b

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: scott

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666510

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: MI.M05812_0285.001.IDT_i7_147---IDT_i5_147.UTM7_1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666511

text: Chlamydomonas reinhardtii

characteristic: organism

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_111---IDT_i5_111.CL3_1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666512

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_123---IDT_i5_123.JG4_1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666513

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_135---IDT_i5_135.JG5_1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666514

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_147---IDT_i5_147.UTM7_1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666515

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_171---IDT_i5_171.UW13_2


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666516

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_182---IDT_i5_182.BMS1


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666517

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: NS.1843.002.IDT_i7_25---IDT_i5_25.UW13_1_


text: scott

text: public

text: ena-SAMPLE-TAB-22-09-2022-20:10:27:915-666518

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: Universite de Strasbourg

text: public

tag: Namespace:Universite de Strasbourg

text: raw reads Pacbio, complete genome sequence of Aminobacter niigataensis strain MD1 isolated from wastewater sludge

text: Aminobacter niigataensis

text: Aminobacter niigataensis

characteristic: strain

text: Aminobacter niigataensis strain MD1, raw reads Pacbio


text: UNIVERSIDAD DE JAEN

text: public

text: Genome of antimicrobial resistant strain from food

text: Escherichia coli

text: Escherichia coli


text: STATENS SERUM INSTITUT

text: public

text: Staphylococcus haemolyticus

text: Staphylococcus haemolyticus


text: Goethe University Frankfurt

text: public

tag: Namespace:Goethe University Frankfurt

text: Lisa M. Schulte

text: Brown's leopard frog

characteristic: common name

text: Lithobates brownorum male nuptial pad

text: Mexico

characteristic: geographic location (country and/or sea)

text: Chiapas

characteristic: geographic location (region and locality)

text: Lithobates brownorum

text: Lithobates brownorum

text: male

text: nuptial pad (thumb)

text: Lithobates brownorum nuptial pad RNAseq


tag: attribute

characteristic: ENA-LAST-UPDATE

text: Goethe University Frankfurt

text: public

text: Lisa M. Schulte

text: highland frog

text: Lithobates maculatus male nuptial pad

text: Mexico

text: Chiapas

text: Lithobates maculatus

text: Lithobates maculatus

text: male

characteristic: sex

text: nuptial pad (thumb)

text: Lithobates maculatus nuptial pad RNAseq


characteristic: External Id

text: Goethe University Frankfurt

characteristic: INSDC center name

text: public

text: Lisa M. Schulte

text: Vaillant's frog

text: Lithobates vaillanti male nuptial pad

text: Mexico

text: Chiapas

text: Lithobates vaillanti

text: Lithobates vaillanti

text: male

text: nuptial pad (thumb)

text: Lithobates vaillanti nuptial pad RNAseq


text: Clinical Bioinformatics Area

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

tag: Namespace:Clinical Bioinformatics Area

text: Servicio de Microbiologia, Hospital Virgen de las Nieves, Granada, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: AND13723, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13725, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND13726, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND13729, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13730, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND13731, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND13732, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13733, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND13734, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND13735, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13737, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND13738, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND13739, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13740, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: Servicio de Microbiologia, Hospital Virgen de las Nieves, Granada, Spain

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND13741, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

text: active surveillance in response to outbreak

characteristic: sample capture status

text: AND13743, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

characteristic: Submitter Id

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND13744, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

characteristic: broker name

text: resting

characteristic: cell cycle stage

text: CD4-positive helper T cell

text: human

characteristic: common name

text: Protocols: Isolation of T cells from blood: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Plaque Plus (GE Healthcare, UK) density gradient centrifugation. Human CD4+ T cells were isolated from PBMC by EasySep positive selection kit (Stemcell Technologies) according to manufacturer’s instruction. Positively selected CD4+ T cells were washed with PBS and stained for subsequent Th cell subset sorting. Effector memory Th9 (CXCR3-CCR8+CCR6-CCR4+) and Th2 (CXCR3-CCR8-CCR6-CCR4+) were sorted to over 90% purity according to their expression of chemokine receptors from CD45RA-CD25-CD8-CD3+ cells. Single cell Th9 clones were directly sorted into 96-well plate according to their expression of chemokine receptors. After T cell single cell cloning, cells were screened for IL-9R expression by flow cytometry. Isolation of T cells from skin biopsies: Skin biopsies from positive patch test reactions were cultured in culture medium and CD4+ T cells were isolated and screened for IL-9R expression by flow cytometry. Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 5 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96-well plate in a total volume of 200μL cell culture medium. IL-9R+ T helper cells were incubated with or without IL-9 (5ng/ml) for 12 hours. Total RNA was isolated with RNAeasy kit (Qiagen). Followed Illumina TruSeq Stranded mRNA protocol.

text: adult

characteristic: developmental stage

text: normal

characteristic: disease

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: resting

text: CD4-positive helper T cell

text: human

characteristic: description

text: adult

text: allergic contact dermatitis

text: Homo sapiens

text: Homo sapiens

text: zone of skin

text: Homo sapiens

text: male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: age

text: ArrayExpress

text: resting

text: CD4-positive helper T cell

text: human

text: adult

text: allergic contact dermatitis

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: zone of skin

text: Homo sapiens

text: female

characteristic: title


text: Anopheles gambiae

characteristic: ArrayExpress-SPECIES

text: SC

text: public

text: African malaria mosquito

characteristic: common name

text: Anopheles gambiae

text: Anopheles gambiae


text: University of Luebeck

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672589

tag: Namespace:University of Luebeck

text: vagina

characteristic: broad-scale environmental context

text: not provided

characteristic: collection date

text: human

text: vagina

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: vagina

characteristic: host body site

text: vagina

characteristic: local environmental context

text: Homo sapiens

text: negative

text: Nanopore vaginal microbiome in ART

characteristic: project name

text: Homo sapiens

text: NGS

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Luebeck

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672590

characteristic: Submitter Id

text: vagina

text: not provided

text: human

text: vagina

text: Germany

text: vagina

text: vagina

text: Homo sapiens

text: negative

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS


text: University of Luebeck

characteristic: INSDC last update

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672591

text: vagina

characteristic: broad-scale environmental context

text: not provided

characteristic: collection date

text: human

characteristic: common name

text: vagina

characteristic: environmental medium

text: Germany

text: vagina

text: vagina

text: Homo sapiens

characteristic: organism

text: negative

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS

characteristic: title


text: University of Luebeck

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672592

text: vagina

text: not provided

text: human

text: vagina

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: vagina

characteristic: host body site

text: vagina

characteristic: local environmental context

text: Homo sapiens

text: negative

text: Nanopore vaginal microbiome in ART

characteristic: project name

text: Homo sapiens

text: NGS

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Luebeck

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672593

text: vagina

text: not provided

text: human

text: vagina

text: Germany

text: vagina

text: vagina

text: Homo sapiens

text: negative

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS


text: University of Luebeck

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672594

characteristic: Submitter Id

text: vagina

characteristic: broad-scale environmental context

text: not provided

characteristic: collection date

text: human

characteristic: common name

text: vagina

characteristic: environmental medium

text: Germany

text: vagina

text: vagina

text: Homo sapiens

text: positive

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS


text: University of Luebeck

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672595

text: vagina

text: not provided

text: human

text: vagina

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: vagina

characteristic: host body site

text: vagina

characteristic: local environmental context

text: Homo sapiens

characteristic: organism

text: negative

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Luebeck

characteristic: INSDC center name

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672596

text: vagina

text: not provided

text: human

text: vagina

text: Germany

text: vagina

text: vagina

text: Homo sapiens

text: negative

text: Nanopore vaginal microbiome in ART

characteristic: project name

text: Homo sapiens

text: NGS

characteristic: sequencing method


text: University of Luebeck

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672597

characteristic: Submitter Id

text: vagina

characteristic: broad-scale environmental context

text: not provided

characteristic: collection date

text: human

text: vagina

text: Germany

text: vagina

text: vagina

text: Homo sapiens

text: positive

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS


text: University of Luebeck

text: public

text: ena-SAMPLE-TAB-23-09-2022-12:39:55:809-672598

text: vagina

text: not provided

text: human

characteristic: common name

text: vagina

characteristic: environmental medium

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: vagina

characteristic: host body site

text: vagina

characteristic: local environmental context

text: Homo sapiens

text: positive

text: Nanopore vaginal microbiome in ART

text: Homo sapiens

text: NGS