characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14211/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


status: PUBLIC

tag: attribute

characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

tag: Namespace:Laboratory of genomics and metagenomics, Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: EHNV, CH-1400 Yverdon, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Trestan Pillonel, Damien Jacot, Sébastien Aeby, Gilbert Greub, Claire Bertelli

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14284/2022

text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak

text: active surveillance not initiated by an outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Laboratory of genomics and metagenomics, Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14194/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Hôpital Cantonal de Fribourg - HFR, CH-1752 Villars-sur-Glâne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14380/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14203/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14352/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14187/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14333/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14205/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14165/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14192/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14282/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: EHNV, CH-1400 Yverdon, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Trestan Pillonel, Damien Jacot, Sébastien Aeby, Gilbert Greub, Claire Bertelli

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14185/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14177/2022

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14171/2022

characteristic: isolate

characteristic: organism

text: other

characteristic: sample capture status

text: active surveillance in response to outbreak

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

text: Laboratory of genomics and metagenomics, Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14197/2022

text: active surveillance not initiated by an outbreak


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14283/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14195/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Hôpital Cantonal de Fribourg - HFR, CH-1752 Villars-sur-Glâne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14383/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14191/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14347/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14188/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14281/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Trestan Pillonel, Damien Jacot, Sébastien Aeby, Gilbert Greub, Claire Bertelli

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14296/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14300/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14149/2022

text: Severe acute respiratory syndrome coronavirus 2


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14340/2022

characteristic: isolate

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

text: Laboratory of genomics and metagenomics, Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: EHNV, CH-1400 Yverdon, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14200/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14337/2022

text: active surveillance not initiated by an outbreak


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: EHNV, CH-1400 Yverdon, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14291/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14295/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14206/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14159/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: CHUV, CH-1011 Lausanne, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14319/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: INSDC last update

text: public

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: VD

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

text: hCoV-19/Switzerland/VD-CHUV-GEN14302/2022


characteristic: External Id

characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: SIB

text: CHUV, CH-1011 Lausanne, Switzerland

text: Trestan Pillonel, Damien Jacot, Sébastien Aeby, Gilbert Greub, Claire Bertelli

text: Switzerland

text: VD

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/VD-CHUV-GEN14204/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Zurich

characteristic: INSDC last update

text: public

characteristic: SRA accession

tag: Namespace:University of Zurich

characteristic: Submitter Id

text: Switzerland

text: not collected

text: Homo sapiens

text: clinical sample

text: Corynebacterium diphtheriae

text: Corynebacterium diphtheriae

text: Diphtheria In Europe 2022


text: ROBERT KOCH INSTITUT

text: public

tag: Namespace:ROBERT KOCH INSTITUT

characteristic: collection date

text: isolate obtained from a human listeriosis case in Germany

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: human listeriosis

text: Listeria monocytogenes

text: Listeria monocytogenes

text: IVb

characteristic: serovar

text: clinical isolate of L. monocytogenes

characteristic: strain

text: clinical isolate of L. monocytogenes


characteristic: External Id

text: NIPH

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ELIXIR-Norway

characteristic: broker name

text: N. meningitidis

characteristic: common name

text: Norway

text: not provided

text: Homo sapiens

characteristic: isolate

text: eye secretion

text: Neisseria meningitidis

text: Neisseria meningitidis


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NIPH

characteristic: INSDC last update

text: public

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

text: ear

text: Streptococcus pyogenes

characteristic: organism

text: Streptococcus pyogenes

characteristic: title


text: NIPH

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapiens

text: Blood

text: Streptococcus pyogenes

text: Streptococcus pyogenes


characteristic: External Id

text: NIPH

characteristic: INSDC center name

text: public

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

characteristic: host scientific name

text: Cerebrospinal fluid

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: NIPH

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ELIXIR-Norway

characteristic: broker name

text: S. pyogenes

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

text: Abscess/tissue sample

text: Streptococcus pyogenes

text: Streptococcus pyogenes


characteristic: ENA-CHECKLIST

text: NIPH

text: public

text: ELIXIR-Norway

text: S. pyogenes

characteristic: common name

text: GAS routine sample

text: Norway

text: not provided

text: Homo sapiens

characteristic: isolate

text: joint fluid

text: Streptococcus pyogenes

text: Streptococcus pyogenes


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

characteristic: INSDC last update

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243786 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Bacteroidales bacterium

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

characteristic: project name

characteristic: sample derived from

text: uncultured Bacteroidales bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243786_bin.18_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243786_bin.7_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243787 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Muribaculaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Muribaculaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243787_bin.10_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Dubosiella newyorkensis

characteristic: organism

text: Dubosiella newyorkensis

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Dubosiella;s__Dubosiella newyorkensis

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243787_bin.17_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243787_bin.24_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243788 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Muribaculaceae bacterium

characteristic: organism

text: uncultured Muribaculaceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243788_bin.14_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243788_bin.20_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Alistipes sp.

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Alistipes sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243788_bin.26_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243788_bin.37_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243789 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243789_bin.14_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Clostridioides difficile

characteristic: project name

characteristic: sample derived from

text: Clostridioides difficile

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Peptostreptococcaceae;g__Clostridioides;s__Clostridioides difficile

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243789_bin.2_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243789_bin.20_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243789_bin.3_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Enterococcus gallinarum

characteristic: organism

text: Enterococcus gallinarum

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Enterococcaceae;g__Enterococcus;s__Enterococcus gallinarum

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243789_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243789_bin.8_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243790 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243790_bin.11_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Rikenellaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Rikenellaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Millionella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243790_bin.17_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243790_bin.18_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: [Clostridium] symbiosum

characteristic: project name

characteristic: sample derived from

text: [Clostridium] symbiosum

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__[Clostridium] symbiosum

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243790_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243790_bin.7_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Blautia coccoides

characteristic: project name

characteristic: sample derived from

text: Blautia coccoides

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Blautia;s__Blautia coccoides

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243790_bin.9_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243791 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.10_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Alistipes sp.

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

characteristic: project name

characteristic: sample derived from

text: uncultured Alistipes sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243791_bin.15_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.24_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243791_bin.25_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.26_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Alistipes sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Alistipes sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243791_bin.41_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Muribaculaceae bacterium

characteristic: organism

text: uncultured Muribaculaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.42_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Pseudoflavonifractor sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Pseudoflavonifractor sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Pseudoflavonifractor;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243791_bin.49_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.57_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243791 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243791_bin.60_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243791_bin.61_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243792 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243792_bin.11_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Flavonifractor sp.

characteristic: organism

text: uncultured Flavonifractor sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Flavonifractor;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243792_bin.13_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lactobacillus sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lactobacillus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus murinus

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243792_bin.15_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Lactobacillus johnsonii

characteristic: organism

text: Lactobacillus johnsonii

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus johnsonii

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243792_bin.3_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured proteobacterium

characteristic: project name

characteristic: sample derived from

text: uncultured proteobacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Deltaproteobacteria;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243792_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: [Clostridium] innocuum

characteristic: organism

text: [Clostridium] innocuum

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Erysipelatoclostridium;s__[Clostridium] innocuum

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243792_bin.6_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Robinsoniella sp.

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

characteristic: project name

characteristic: sample derived from

text: uncultured Robinsoniella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Robinsoniella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243792_bin.9_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243793 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243793_bin.11_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Eubacterium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243793_bin.17_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Odoribacter sp.

characteristic: organism

text: uncultured Odoribacter sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Odoribacteraceae;g__Odoribacter;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243793_bin.19_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243793_bin.2_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243793_bin.26_metawrap_v1.3_MAG

characteristic: title