characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1044

text: human

text: gut

text: feces

text: Netherlands

characteristic: geographic location (latitude)

text: feces

characteristic: host subject id

text: human-gut

text: human gut metagenome

characteristic: organism

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

tag: Namespace:Maastricht University

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1045

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (longitude)

unit: month

characteristic: host age

text: feces

characteristic: host body product

text: human-gut

characteristic: human gut environmental package

characteristic: investigation type

text: human gut metagenome

text: GTGCCAGC[AC]GCCGCGGTAA GGACTAC[ACT][ACG]GGGT[AT]TCTAAT

characteristic: pcr primers

text: KOALA Birth Cohort Study

characteristic: project name

text: human gut metagenome

text: Illumina HiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1046

text: human

text: gut

text: feces

text: Netherlands

characteristic: geographic location (latitude)

text: feces

characteristic: host subject id

text: human-gut

text: human gut metagenome

characteristic: organism

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1047

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

characteristic: host age

text: feces

characteristic: host body product

text: human-gut

characteristic: human gut environmental package

text: human gut metagenome

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1048

text: human

text: gut

text: feces

text: Netherlands

text: feces

text: human-gut

characteristic: investigation type

text: human gut metagenome

characteristic: pcr primers

text: KOALA Birth Cohort Study

characteristic: project name

text: human gut metagenome

text: Illumina HiSeq

characteristic: sequencing method


characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1049

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: feces

characteristic: host subject id

text: human-gut

text: human gut metagenome

characteristic: organism

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1050

text: human

text: gut

text: feces

text: Netherlands

characteristic: geographic location (longitude)

characteristic: host age

text: feces

characteristic: host body product

text: human-gut

characteristic: human gut environmental package

text: human gut metagenome

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq


characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1051

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

text: feces

text: human-gut

characteristic: investigation type

text: human gut metagenome

characteristic: pcr primers

text: KOALA Birth Cohort Study

characteristic: project name

text: human gut metagenome

text: Illumina HiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1052

text: human

text: gut

text: feces

text: Netherlands

characteristic: geographic location (latitude)

text: feces

characteristic: host subject id

text: human-gut

text: human gut metagenome

characteristic: organism

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1053

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (longitude)

characteristic: host age

text: feces

characteristic: host body product

text: human-gut

characteristic: human gut environmental package

text: human gut metagenome

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Maastricht University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1054

text: human

text: gut

text: feces

text: Netherlands

text: feces

text: human-gut

characteristic: investigation type

text: human gut metagenome

characteristic: pcr primers

text: KOALA Birth Cohort Study

characteristic: project name

text: human gut metagenome

text: Illumina HiSeq

characteristic: sequencing method


characteristic: ENA-LAST-UPDATE

text: Maastricht University

text: public

characteristic: INSDC status

characteristic: collection date

text: Fecal microbiome (Illumina HiSeq, 16S, 515F-806R primers) of KOALA birth cohort study participant at 1 month old. Infant 1055

characteristic: description

text: human

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: feces

characteristic: host subject id

text: human-gut

text: human gut metagenome

characteristic: organism

text: KOALA Birth Cohort Study

text: human gut metagenome

text: Illumina HiSeq

characteristic: title


control

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: control

tag: Namespace:ADAM MICKIEWICZ UNIVERSITY, POZNAN, POLAND

characteristic: Submitter Id

text: thale cress

characteristic: common name

text: Control sample grown in standard conditions

text: Poland

text: not provided

text: not provided

characteristic: geographic location (longitude)

text: standard soil growth conditions

characteristic: isolation and growth condition

text: Arabidopsis thaliana

text: 21 day

characteristic: plant developmental stage

text: soil

characteristic: plant growth medium

text: leaf

characteristic: plant structure

text: Arabidopsis thaliana

text: control


TXPTS

characteristic: ENA-LAST-UPDATE

text: ADAM MICKIEWICZ UNIVERSITY, POZNAN, POLAND

text: public

characteristic: INSDC status

text: TXPTS

characteristic: collection date

text: thale cress

text: TXPTS-treaded sample

characteristic: description

text: Poland

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

text: standard soil growth conditions

text: Arabidopsis thaliana

text: 21 day

text: soil

text: leaf

text: TXPTS

characteristic: plant treatment

text: Arabidopsis thaliana

text: TXPTS


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF GLASGOW

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:UNIVERSITY OF GLASGOW

text: GBS from human in Vietnam

text: Viet Nam

text: diseased

characteristic: host health state

text: Homo sapiens sapiens

characteristic: host scientific name

characteristic: isolate

text: human

characteristic: isolation source

text: Streptococcus agalactiae

characteristic: organism

text: Streptococcus agalactiae

characteristic: title


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF GLASGOW

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: GBS from fish in Vietnam

text: Viet Nam

text: diseased

text: Oreochromis niloticus

text: fish

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: UNIVERSITY OF GLASGOW

text: public

characteristic: collection date

text: GBS from bovine in Vietnam

characteristic: description

text: Viet Nam

characteristic: geographic location (country and/or sea)

text: diseased

text: Bos taurus

text: bovine

text: Streptococcus agalactiae

text: Streptococcus agalactiae


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: UNIVERSITY OF GLASGOW

text: public

text: GBS from fish in Thailand

text: Thailand

text: diseased

text: Oreochromis niloticus

text: fish

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: University of Zurich

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:University of Zurich

text: See sample_title

text: Albizia petersiana

text: Albizia petersiana

text: Greenway & Kanuri 15362 (PRE)

characteristic: specimen voucher

text: Hybrid capture sequence reads of Albizia petersiana. Voucher: Greenway & Kanuri 15362 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia evansii

text: Albizia evansii

text: Correia & Marques 843 (PRE)

text: Hybrid capture sequence reads of Albizia evansii. Voucher: Correia & Marques 843 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia gummifera

text: Albizia gummifera

text: Tawakali & Kaunda 436 (PRE)

text: Hybrid capture sequence reads of Albizia gummifera. Voucher: Tawakali & Kaunda 436 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia mainaea

text: Albizia mainaea

text: Du Puy M682 (K)

text: Hybrid capture sequence reads of Albizia mainaea. Voucher: Du Puy M682 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia laurentii

text: Albizia laurentii

text: Breteler 15764 (WAG)

text: Hybrid capture sequence reads of Albizia laurentii. Voucher: Breteler 15764 (WAG)


text: University of Zurich

text: public

text: See sample_title

text: Albizia gillardinii

text: Albizia gillardinii

text: Deschamps 256 (K)

text: Hybrid capture sequence reads of Albizia gillardinii. Voucher: Deschamps 256 (K)


text: University of Zurich

text: public

text: silk tree

characteristic: common name

text: See sample_title

text: Albizia julibrissin

text: Albizia julibrissin

text: Fumihiro Konta 18313 (P)

text: Hybrid capture sequence reads of Albizia julibrissin. Voucher: Fumihiro Konta 18313 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia kalkora

characteristic: organism

text: Albizia kalkora

text: Xiao Bai-Zhong (K)

text: Hybrid capture sequence reads of Albizia kalkora. Voucher: Xiao Bai-Zhong (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia chinensis

text: Albizia chinensis

text: But 79-100A (K)

text: Hybrid capture sequence reads of Albizia chinensis. Voucher: But 79-100A (K)

characteristic: title


characteristic: ENA-LAST-UPDATE

text: University of Zurich

text: public

text: See sample_title

text: Albizia philippinensis

text: Albizia philippinensis

text: Ahern’s collector 215 (P)

text: Hybrid capture sequence reads of Albizia philippinensis. Voucher: Ahern’s collector 215 (P)


text: University of Zurich

characteristic: INSDC last update

text: public

text: See sample_title

text: Albizia sherriffii

text: Albizia sherriffii

text: Grierson 4314 (K)

text: Hybrid capture sequence reads of Albizia sherriffii. Voucher: Grierson 4314 (K)


text: University of Zurich

text: public

characteristic: INSDC status

text: See sample_title

text: Albizia salomonensis

text: Albizia salomonensis

text: Whitmore BSIP 4424 (K)

text: Hybrid capture sequence reads of Albizia salomonensis. Voucher: Whitmore BSIP 4424 (K)


text: University of Zurich

text: public

characteristic: SRA accession

text: See sample_title

text: Albizia guillainii

text: Albizia guillainii

text: Veillon 8151 (NOU)

text: Hybrid capture sequence reads of Albizia guillainii. Voucher: Veillon 8151 (NOU)


text: University of Zurich

text: public

characteristic: Submitter Id

text: See sample_title

text: Albizia lebbekoides

text: Albizia lebbekoides

text: Larsen 31706 (K)

text: Hybrid capture sequence reads of Albizia lebbekoides. Voucher: Larsen 31706 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia vialeana

text: Albizia vialeana

text: Larsen 32204 (P)

text: Hybrid capture sequence reads of Albizia vialeana. Voucher: Larsen 32204 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia odoratissima

text: Albizia odoratissima

text: Chow 80078 (K)

text: Hybrid capture sequence reads of Albizia odoratissima. Voucher: Chow 80078 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia corniculata

text: Albizia corniculata

text: Shiu Ying Hu 10383 (K)

text: Hybrid capture sequence reads of Albizia corniculata. Voucher: Shiu Ying Hu 10383 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia rufa

text: Albizia rufa

text: Zollinger 80 (P)

text: Hybrid capture sequence reads of Albizia rufa. Voucher: Zollinger 80 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia myriophylla

text: Albizia myriophylla

text: Larsen 33455 (P)

text: Hybrid capture sequence reads of Albizia myriophylla. Voucher: Larsen 33455 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia tomentella

text: Albizia tomentella

text: Leach & Dunlop 3801 (L)

text: Hybrid capture sequence reads of Albizia tomentella. Voucher: Leach & Dunlop 3801 (L)


text: University of Zurich

text: public

text: See sample_title

text: Albizia crassiramea

text: Albizia crassiramea

text: Parnell 95-401 (K)

text: Hybrid capture sequence reads of Albizia crassiramea. Voucher: Parnell 95-401 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia duclouxii

text: Albizia duclouxii

text: Ducloux 6112 (P)

text: Hybrid capture sequence reads of Albizia duclouxii. Voucher: Ducloux 6112 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia lebbeck

text: Albizia lebbeck

text: Nee 28682 (NY)

text: Hybrid capture sequence reads of Albizia lebbeck. Voucher: Nee 28682 (NY)


text: University of Zurich

text: public

text: See sample_title

text: Albizia procera

text: Albizia procera

text: Matthew RHT 63670 (K)

text: Hybrid capture sequence reads of Albizia procera. Voucher: Matthew RHT 63670 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia attopeuensis

text: Albizia attopeuensis

text: Maxwell 92-747 (P)

text: Hybrid capture sequence reads of Albizia attopeuensis. Voucher: Maxwell 92-747 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia canescens

text: Albizia canescens

text: Forster PIF9580 (L)

text: Hybrid capture sequence reads of Albizia canescens. Voucher: Forster PIF9580 (L)


text: University of Zurich

text: public

text: See sample_title

text: Albizia pedicellata

text: Albizia pedicellata

text: Ambriansyah AA702 (K)

text: Hybrid capture sequence reads of Albizia pedicellata. Voucher: Ambriansyah AA702 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia rosulata

text: Albizia rosulata

text: Ariffin Kalat BRUN 17375 (K)

text: Hybrid capture sequence reads of Albizia rosulata. Voucher: Ariffin Kalat BRUN 17375 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia acle

text: Albizia acle

text: Villavicencio 27204 (P)

text: Hybrid capture sequence reads of Albizia acle. Voucher: Villavicencio 27204 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia chevalieri

text: Albizia chevalieri

text: Okafov & Daramota 54622 (FHO)

text: Hybrid capture sequence reads of Albizia chevalieri. Voucher: Okafov & Daramota 54622 (FHO)


text: University of Zurich

text: public

text: See sample_title

text: Albizia harveyi

text: Albizia harveyi

text: Maurin 0773 (JRAU)

text: Hybrid capture sequence reads of Albizia harveyi. Voucher: Maurin 0773 (JRAU)


text: University of Zurich

text: public

text: See sample_title

text: Albizia suluensis

text: Albizia suluensis

text: Maurin 1810 (JRAU)

text: Hybrid capture sequence reads of Albizia suluensis. Voucher: Maurin 1810 (JRAU)


text: University of Zurich

text: public

text: See sample_title

text: Albizia malacophylla

text: Albizia malacophylla

text: de Wilde & de Wilde-Duyfjes 8944 (PRE)

text: Hybrid capture sequence reads of Albizia malacophylla. Voucher: de Wilde & de Wilde-Duyfjes 8944 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia antunesiana

text: Albizia antunesiana

text: Tinley 1454 (PRE)

text: Hybrid capture sequence reads of Albizia antunesiana. Voucher: Tinley 1454 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia tanganyicensis

text: Albizia tanganyicensis

text: Maurin 1972 (JRAU)

text: Hybrid capture sequence reads of Albizia tanganyicensis. Voucher: Maurin 1972 (JRAU)


text: University of Zurich

text: public

text: See sample_title

text: Albizia coriaria

text: Albizia coriaria

text: Jongkind 2482 (WAG)

text: Hybrid capture sequence reads of Albizia coriaria. Voucher: Jongkind 2482 (WAG)


text: University of Zurich

text: public

text: See sample_title

text: Albizia odorata

text: Albizia odorata

text: Du Puy M335 (K)

text: Hybrid capture sequence reads of Albizia odorata. Voucher: Du Puy M335 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia tulearensis

text: Albizia tulearensis

text: Hybrid capture sequence reads of Albizia tulearensis. Voucher: Service Forestier Madagascar & Capuron 29107 sf (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia vaughanii

text: Albizia vaughanii

text: Vaughan 10763 (K)

text: Hybrid capture sequence reads of Albizia vaughanii. Voucher: Vaughan 10763 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia divaricata

text: Albizia divaricata

text: Randrianaivo 485 (P)

text: Hybrid capture sequence reads of Albizia divaricata. Voucher: Randrianaivo 485 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia forbesii

text: Albizia forbesii

text: Maurin 0228 (JRAU)

text: Hybrid capture sequence reads of Albizia forbesii. Voucher: Maurin 0228 (JRAU)


text: University of Zurich

text: public

text: See sample_title

text: Albizia morombensis

text: Albizia morombensis

text: Phillipson & Manjakahery 5985 (P)

text: Hybrid capture sequence reads of Albizia morombensis. Voucher: Phillipson & Manjakahery 5985 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia numidarum

text: Albizia numidarum

text: Labat 3552 (K)

text: Hybrid capture sequence reads of Albizia numidarum. Voucher: Labat 3552 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia arenicola

text: Albizia arenicola

text: Du Puy M296 (K)

text: Hybrid capture sequence reads of Albizia arenicola. Voucher: Du Puy M296 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia boinensis

text: Albizia boinensis

text: Du Puy M523 (WAG)

text: Hybrid capture sequence reads of Albizia boinensis. Voucher: Du Puy M523 (WAG)


text: University of Zurich

text: public

text: See sample_title

text: Albizia verrucosa

text: Albizia verrucosa

text: Hybrid capture sequence reads of Albizia verrucosa. Voucher: Service Forestier Madagascar & Capuron 24093 sf (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia perrieri

text: Albizia perrieri

text: Nusbaumer LN 1876 (K)

text: Hybrid capture sequence reads of Albizia perrieri. Voucher: Nusbaumer LN 1876 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia androyensis

text: Albizia androyensis

text: Randrianaivo et al. 1742 (P)

text: Hybrid capture sequence reads of Albizia androyensis. Voucher: Randrianaivo et al. 1742 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia commiphoroides

text: Albizia commiphoroides

text: Phillipson 2781 (P)

text: Hybrid capture sequence reads of Albizia commiphoroides. Voucher: Phillipson 2781 (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia balabaka

text: Albizia balabaka

text: Du Puy M123 (K)

text: Hybrid capture sequence reads of Albizia balabaka. Voucher: Du Puy M123 (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia jaubertiana

text: Albizia jaubertiana

text: Hybrid capture sequence reads of Albizia jaubertiana. Voucher: Service Forestier Madagascar & Capuron 24619 sf (P)


text: University of Zurich

text: public

text: See sample_title

text: Albizia isenbergiana

text: Albizia isenbergiana

text: Verdcourt 3237B (K)

text: Hybrid capture sequence reads of Albizia isenbergiana. Voucher: Verdcourt 3237B (K)


text: University of Zurich

text: public

text: See sample_title

text: Albizia schimperiana

text: Albizia schimperiana

text: Van Wyk BSA 2748 (PRE)

text: Hybrid capture sequence reads of Albizia schimperiana. Voucher: Van Wyk BSA 2748 (PRE)


text: University of Zurich

text: public

text: See sample_title

text: Albizia glaberrima

text: Albizia glaberrima

text: Maurin 2605 (JRAU)

text: Hybrid capture sequence reads of Albizia glaberrima. Voucher: Maurin 2605 (JRAU)


text: University of Zurich

text: public

text: See sample_title

text: Albizia amara subsp. sericocephala

text: Albizia amara subsp. sericocephala

text: Maurin 3368 (JRAU)

text: Hybrid capture sequence reads of Albizia amara subsp. sericocephala. Voucher: Maurin 3368 (JRAU)


text: UMC Utrecht

text: public

tag: Namespace:UMC Utrecht

text: human

text: 16S rRNA gene sequencing of uterine biopsy

text: Homo sapiens

text: Homo sapiens


text: public

tag: Namespace:Computational Biology and Microbial Ecology

characteristic: collection date

text: Vibrio parahaemolyticus causing AHPND isolate from shrimp stomach and hepatopancreas

text: Ecuador

text: diseased

characteristic: host health state

text: Penaeus (Litopenaeus) vannamei

characteristic: host scientific name

characteristic: isolate

text: Macerated stomach and hepatopancreas samples from shrimp. Samples were grown in TCBS and colonies purified on TSA plates according to morphology. Isolate was cultured in TCBS.

characteristic: isolation source

text: Vibrio parahaemolyticus

text: Vibrio parahaemolyticus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Computational Biology and Microbial Ecology

text: public

characteristic: description

text: Ecuador

characteristic: geographic location (country and/or sea)

text: diseased

text: Penaeus (Litopenaeus) vannamei

text: Vibrio parahaemolyticus

text: Vibrio parahaemolyticus

text: Vibrio parahaemolyticus causing AHPND isolate from shrimp


Undetermined

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Undetermined

text: Ecuador

text: diseased

text: Penaeus (Litopenaeus) vannamei

text: Vibrio parahaemolyticus

characteristic: organism

text: Vibrio parahaemolyticus


characteristic: ENA-LAST-UPDATE

text: INSERM Gustave Roussy Institute

characteristic: INSDC last update

text: public

tag: Namespace:INSERM Gustave Roussy Institute

text: ArrayExpress

characteristic: broker name

text: Non applicable

characteristic: cell line

text: CD34-negative, CD41-positive, CD42-positive megakaryocyte cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cut&Tag against GATA1 was performed on CD41+CD42+ megakaryocytes obtained after 18 days of differentiation of the IPSC clones. 500,000 CD41+CD42+ MK sorted cells were used to analyze GATA1 and GATA1s chromatin occupancy using the CUT&Tag-IT Assay Kit (Active Motif) according to the manufacturer recommendations. Briefly, cells were bound to Concanavalin A-Coated Beads and incubated with primary anti-GATA1 antibody in buffer with Protease Inhibitor Cocktail and 5% digitonine overnight at 4°C under rotation. The Guinea Pig anti-rabbit secondary antibody was incubated in Dig-Wash buffer for 1 hour at RT under rotation. After 3 washes, the CUT&Tag-IT™ Assembled pA-Tn5 Transposomes (1:100) were added for 1 hour at RT under rotation and tagmentation was performed during 1 hour at 37°C. DNA was purified. Libraries were generated by PCR as for for all Tn5 based libraries. The final libraries were purified, pooled together in equal concentrations

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: Trisomic with GATA1s mutation

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: INSERM Gustave Roussy Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Non applicable

text: human

text: Down syndrome

text: Trisomic

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: INSERM GUSTAVE ROUSSY Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:INSERM GUSTAVE ROUSSY Institute

text: ArrayExpress

text: Non applicable

text: hematopoietic cell from IPSC

text: human

text: Protocols: Hematopoietic cells in the supernatant of cultures at day 13 of differentiation were purified for cell viability using Sytox blue. Sample preparation was done at room temperature. Single-cell suspensions were loaded onto a Chromium Single Cell Chip (10x Genomics) according to the manufacturer’s instructions for co-encapsulation with barcoded Gel Beads at a target capture rate of ~10,000 individual cells per sample. Captured mRNAs were barcoded during cDNA synthesis using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics) according to the manufacturer’s instructions. All samples were processed simultaneously with the Chromium Controller (10X Genomics) and the resulting libraries were prepared in parallel in a single batch.

characteristic: description

text: embryo stage

text: control

text: disomic control

text: Myeloid, Erythroid, Megakaryocyte

characteristic: inferred cell type

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Non applicable

text: Homo sapiens


text: INSERM GUSTAVE ROUSSY Institute

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: Non applicable

characteristic: cell line

text: hematopoietic cell from IPSC

characteristic: cell type

text: human

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Down syndrome

text: disomic with GATA1s mutation,MPL mutation and SMC3 mutation

text: Myeloid, Erythroid, Megakaryocyte

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM GUSTAVE ROUSSY Institute

text: public

text: ArrayExpress

text: Non applicable

text: hematopoietic cell from IPSC

text: human

text: embryo stage

text: Down syndrome

characteristic: disease

text: trisomic with GATAs mutation and the SMC3 mutation

characteristic: genotype

text: Myeloid, Erythroid, Megakaryocyte

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSERM GUSTAVE ROUSSY Institute

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Non applicable

text: hematopoietic cell from IPSC

text: human

text: embryo stage

text: Down syndrome

text: trisomic with SMC3 mutation

text: Myeloid, Erythroid, Megakaryocyte

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


text: INSERM GUSTAVE ROUSSY Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: Non applicable

text: hematopoietic cell from IPSC

text: human

characteristic: description

text: embryo stage

text: Down syndrome

text: trisomic with mutation GATA1s, an MPL mutation and an SMC3 mutation

text: Myeloid, Erythroid, Megakaryocyte

characteristic: inferred cell type

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM GUSTAVE ROUSSY Institute

text: public

text: ArrayExpress

text: Non applicable

characteristic: cell line

text: hematopoietic cell from IPSC

characteristic: cell type

text: human

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Down syndrome

text: trisomic with mutation GATA1s

text: Myeloid, Erythroid, Megakaryocyte

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSERM GUSTAVE ROUSSY Institute

text: public

text: ArrayExpress

text: Non applicable

text: hematopoietic cell from IPSC

text: human

text: Control

text: Down syndrome

characteristic: disease

text: trisomic

characteristic: genotype

text: Myeloid, Erythroid, Megakaryocyte

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens

characteristic: title


text: INSERM Gustave Roussy institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:INSERM Gustave Roussy institute

characteristic: Submitter Id

text: ArrayExpress

text: Induced pluripotent stem cell

text: Control

text: human

text: Protocols: Cells were labeled with APC, BV421 and PE-conjugated antibodies against CD41a, CD42b and CD33, respectively. In order to obtain a highly purified megakaryocytes, CD33+ cells were removed from the double positive CD41, CD42 population during cell sorting. After sorting, the purity was verified and corresponded to 98-100% of CD41+, CD42+, CD33-. Total RNA was extracted using RNA/DNA/Protein Purification Plus Kit (Proteigene, St Marcel, France). cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).

text: Control

text: Control

text: Disomic with GATA1s mutation and MPL mutation

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM Gustave Roussy institute

text: public

text: ArrayExpress

characteristic: broker name

text: Induced pluripotent stem cell

text: human

characteristic: description

text: embryo stage

text: Down syndrome

text: Disomic with GATA1s mutation MPL mutation

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: INSERM Gustave Roussy institute

text: public

text: ArrayExpress

text: Induced pluripotent stem cell

characteristic: cell line

characteristic: cell type

text: human

characteristic: common name

text: embryo stage

characteristic: developmental stage

text: Down syndrome

text: Disomic with GATA1s mutation MPL mutation and SMC3 mutation

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


E-MTAB-11033:TSMR 77

characteristic: External Id

text: INSERM Gustave Roussy institute

text: public

text: E-MTAB-11033:TSMR 77

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

characteristic: disease

text: Trisomic 21

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens

text: TSMR 77

characteristic: title


E-MTAB-11033:TSMR 78

text: INSERM Gustave Roussy institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11033:TSMR 78

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

text: Trisomic 21

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens

text: TSMR 78


E-MTAB-11033:TSMR 79

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM Gustave Roussy institute

text: public

text: E-MTAB-11033:TSMR 79

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Induced pluripotent stem cell

text: human

characteristic: description

text: embryo stage

text: Down syndrome

text: Trisomic 21

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens

text: TSMR 79


characteristic: ENA-LAST-UPDATE

text: INSERM Gustave Roussy institute

text: public

text: ArrayExpress

text: Induced pluripotent stem cell

characteristic: cell line

characteristic: cell type

text: human

characteristic: common name

text: embryo stage

text: Down syndrome

text: Trisomic 21 with GATA1s mutation

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: External Id

text: INSERM Gustave Roussy institute

text: public

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: Trisomic 21 with GATA1s mutation and MPL mutation

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens


text: INSERM Gustave Roussy institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

text: Trisomic 21 with GATA1s mutation and SMC3 mutation

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM Gustave Roussy institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

text: Trisomic 21 with SMC3 mutation

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

text: INSERM Gustave Roussy institute

text: public

text: ArrayExpress

text: Induced pluripotent stem cell

characteristic: cell line

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: embryo stage

text: Down syndrome

text: Trisomic with GATA1s mutation and MPL mutation

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens


E-MTAB-11033:TSMR 123

characteristic: External Id

text: INSERM Gustave Roussy institute

text: public

text: E-MTAB-11033:TSMR 123

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

characteristic: developmental stage

text: Down syndrome

characteristic: disease

text: Trisomic with GATA1s mutation MPL mutation and SMC3 mutation

characteristic: genotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Non applicable

text: Homo sapiens

text: TSMR 123


E-MTAB-11033:TSMR 124

text: INSERM Gustave Roussy institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11033:TSMR 124

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

text: Homo sapiens

text: Homo sapiens

text: Non applicable

characteristic: organism part

text: Homo sapiens

text: TSMR 124


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: INSERM Gustave Roussy institute

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Induced pluripotent stem cell

text: human

text: embryo stage

text: Down syndrome

text: Homo sapiens

text: Homo sapiens

text: Non applicable

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

tag: Namespace:Department of Molecular Microbiology John Innes Centre

text: ArrayExpress

characteristic: broker name

text: Protocols: A single colony from each conjugation event was picked and grown overnight in 10 mL KB medium with Gentamicin and Kanamycin at 28 degree Celsius, 230 rpm. A single colony from each conjugation event was picked and grown overnight in 10 mL KB medium with Gentamicin and Kanamycin at 28 degree Celsius, 230 rpm. The OD600 of these cultures was measured and 60 mL KB cultures were set up at OD600 = 0.01. Cultures were grown at 28 degree celsius, 230 rpm until OD600 = 1.4. The .bam files produced by aligning the reads to the genome were used as input to the featureCounts tool of the Bioconductor package Rsubread (version 2.0.0) along with a a .saf file containing coordinates of genes in the genome. This produced a table of counts of reads mapping to every gene in the genome. 2 mL of culture was harvested for RNA extraction. Pellets were collected at 8000 xg, 10 minutes at 4 degree Celsius and flash frozen in liquid nitrogen before storage at -80 degree Celsius. Pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol) and cells were lysed using a Fastprep (MP Bio) using Lysis matrix B beads (MP Bio). Lysis matrix was removed by centrifugation (13,000 xg, 3 minutes) and the supernatant was added to 450 µL of ethanol. The supernatant was applied to a RNeasy column and RNA extraction was performed as per the manufacturer’s instruction including the on-column DNA digest. After extraction a Turbo DNase (Promega) digest was performed as per the manufacturer’s instruction and total RNA yield was quantified using a QBIT. Library construction was carried out by an external contractor (Novogene) using the Illumina RNA-Seq library preparation kit.

text: wild type genotype

text: KB medium

characteristic: growth condition

text: Pseudomonas fluorescens

text: Pseudomonas fluorescens

text: whole organism

text: Pseudomonas fluorescens

characteristic: strain


characteristic: External Id

text: Department of Molecular Microbiology John Innes Centre

text: public

text: ArrayExpress

characteristic: description

text: wild type genotype

text: KB medium

text: Pseudomonas fluorescens

text: Pseudomonas fluorescens

text: whole organism

text: Pseudomonas fluorescens


text: public

text: ArrayExpress

characteristic: genotype

text: KB medium

text: Pseudomonas fluorescens

characteristic: organism

text: Pseudomonas fluorescens

text: whole organism

text: Pseudomonas fluorescens


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Hohenheim University - Institute of Plant Nutrition

characteristic: collection date

text: Experiment with Zea maize controlled temperature

characteristic: environment (biome)

text: Oberboden Gr�nfl�che

characteristic: environment (feature)

text: Rhizosphere Soil

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Zea mays

characteristic: project name

text: Zea mays

text: Ilumina sequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Hohenheim University - Institute of Plant Nutrition

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Oberboden Gr�nfl�che

text: Rhizosphere Soil

text: Germany

text: Zea mays

text: Zea mays

text: Ilumina sequencing

text: soil

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection date

characteristic: environment (biome)

text: Oberboden Gr�nfl�che

characteristic: environment (feature)

text: Bulk soil

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: Zea mays

characteristic: organism

text: Zea mays

text: Ilumina sequencing

text: soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: EMBL

text: public

text: baker's yeast

characteristic: common name

text: C-terminally Twin-StrepII tagged Kap114 IP replicate 1

characteristic: description

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


text: EMBL

characteristic: INSDC first public

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap114 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap120 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap122 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Mtr10 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Nmd5 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Sxm1 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap104 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap121 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap123 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Kap95 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: C-terminally Twin-StrepII tagged Srp1 total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait IP replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 2

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 3

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: EMBL

text: public

text: baker's yeast

text: NoBait total replicate 4

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

tag: Namespace:Philipps University of Marburg, Center for Synthetic Microbiology

text: ArrayExpress

characteristic: broker name

text: Protocols: Cells were harvested by centrifugation at 3000g (4°C). S. meliloti cells were grown in TY supplemented with appropriate antibiotics to an optical density 600 (OD600) of 1.0. Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual. DNA libraries for sequencing were generated by applying a Nextera XT DNA Library Preparation kit (Illumina)

text: exponential phase

characteristic: developmental stage

text: SmCreΔhsdR with fusion of secondary replicons pSymA and pSymB

characteristic: genotype

text: TY medium

characteristic: growth condition

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

characteristic: organism part

text: Sinorhizobium meliloti

characteristic: strain

text: SmAB


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Philipps University of Marburg, Center for Synthetic Microbiology

text: public

text: ArrayExpress

text: exponential phase

text: SmCreΔhsdR with fusion of the chromosome, pSmyA and pSymB

text: TY medium

text: Sinorhizobium meliloti

characteristic: organism

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

characteristic: description

text: stationary phase

text: TY medium

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: exponential phase

text: SmABC derivative lacking replication origins of secondary replicons

text: TY medium

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: stationary phase

text: TY medium

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

characteristic: title


characteristic: INSDC first public

text: public

text: ArrayExpress

characteristic: broker name

text: exponential phase

characteristic: developmental stage

text: SmABCΔoriV derivative cured from resistance cassettes

text: TY medium

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

text: SmABCΔoriVΔR


text: public

text: ArrayExpress

text: exponential phase

text: SmABC derivative cured from resistance cassettes

characteristic: genotype

text: TY medium

characteristic: growth condition

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

characteristic: organism part

text: Sinorhizobium meliloti

text: SmABCΔR


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: exponential phase

text: SmAB derivative cured from resistance cassettes

text: TY medium

text: Sinorhizobium meliloti

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

characteristic: strain

text: SmABΔR


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: exponential phase

text: Rm1021 cre expression strain with hsdR deletion, tauX:cre-tetRA

text: TY medium

text: Sinorhizobium meliloti

characteristic: organism

text: Sinorhizobium meliloti

text: whole organism

text: Sinorhizobium meliloti

text: SmCreΔhsdR


Phlebotomus argentipes

text: Liverpool John Moores University

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: Phlebotomus argentipes

tag: Namespace:Liverpool John Moores University

text: Phlebotomus argentipes from a colony maintained at Charles University, Prague, CZ

characteristic: description

text: Phlebotomus argentipes

text: Phlebotomus argentipes

text: Phlebotomus argentipes


text: University of West London

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of West London

characteristic: Submitter Id

characteristic: collection date

text: Armenian Clinical isolate

text: Armenia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

characteristic: isolate

text: Blood

characteristic: isolation source

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Armenian Clinical isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of West London

characteristic: INSDC first public

text: public

text: Armenian Clinical isolate

text: Armenia

text: not provided

text: Homo sapien

text: Stool

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Armenian Clinical isolate

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of West London

text: public

text: Armenian Clinical isolate

text: Armenia

text: not provided

text: Homo sapien

text: throat

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

text: Armenian Clinical isolate


text: University of West London

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: Armenian Clinical isolate

characteristic: description

text: Armenia

text: not provided

text: Homo sapien

text: sputum

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Armenian Clinical isolate


text: University of West London

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Armenian Clinical isolate

text: Armenia

text: not provided

text: Homo sapien

text: CSF

text: Proteus mirabilis

text: Proteus mirabilis

text: Armenian Clinical isolate


text: University of West London

text: public

characteristic: collection date

text: Armenian Clinical isolate

text: Armenia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapien

text: urine

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Armenian Clinical isolate


text: Hospital Sirio-Libanes

text: public

tag: Namespace:Hospital Sirio-Libanes

text: human oral metagenome

characteristic: patient

text: human oral metagenome

text: GCF

characteristic: site

text: P

characteristic: time

text: oral sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Hospital Sirio-Libanes

characteristic: INSDC first public

text: public

text: human oral metagenome

text: human oral metagenome

text: OM

text: A

text: oral sample


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: GCF

text: A

text: oral sample

characteristic: title


text: Hospital Sirio-Libanes

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: human oral metagenome

characteristic: organism

text: human oral metagenome

text: OM

text: E

text: oral sample


text: Hospital Sirio-Libanes

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: description

text: human oral metagenome

text: human oral metagenome

text: GCF

text: E

text: oral sample


text: Hospital Sirio-Libanes

text: public

characteristic: Submitter Id

text: human oral metagenome

text: human oral metagenome

text: OM

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: DB

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: GCF

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: OM

text: P

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: DB

text: P

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: DB

text: A

text: oral sample


text: Hospital Sirio-Libanes

text: public

text: human oral metagenome

text: human oral metagenome

text: DB

text: E

text: oral sample


text: BioMed X GmbH

text: public

tag: Namespace:BioMed X GmbH

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: For protocol 1, we followed the method by Donlin L et al. for dissociation of cryopreserved synovium (steps 1-5): 1. Fresh tissue fragments, obtained by synovial biopsy were minced into small fragments (~1 mm3) using a scalpel. 2. Minced fragments were transferred to U bottom tubes containing a digestion mix ( RPMI culture medium with 100 μg/ml Liberase™ TL (Roche) and 100 μg/ml DNase I (Roche)). 3. Tissues were dissociated with a combination of enzymatic digestion (37°C for 30min) and mechanical disruption (16G syringe needle) under continuous stirring with a magnetic stir bar. 4. The mechanical disruption was performed 15 minutes after enzymatic digestion start by gently passing the samples through a 16G syringe needle (10x). 5. Enzymatic digestion was stopped with RPMI, containing 10% FCS. Thereafter, red blood cells in dissociated synovial cell pellets were lysed using Red Cell Lysis buffer (Roche), according to the manufacturer's instructions and cells were washed with PBS. Single cell 3´protocol (10x Genomics), v3.0/v3.1

text: adult

characteristic: developmental stage

text: arthritis

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: synovial membrane

characteristic: organism part

text: Homo sapiens

text: female

characteristic: sex


tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: BioMed X GmbH

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

text: adult

text: arthritis

text: Homo sapiens

text: Homo sapiens

text: synovial membrane

text: Homo sapiens

text: male


characteristic: External Id

text: BioMed X GmbH

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: ArrayExpress

text: human

text: Protocols: For protocol 2, as described by Edalat G. S. et al. (steps in bold differ between the two protocols): 1. Fresh tissue fragments, obtained by synovial biopsy were placed on 70 μm cell strainers and washed thoroughly in D-PBS to remove the potential sources of non-synovial cell contamination. 2. Washed synovial biopsy fragments were minced into small tissue pieces (~1 mm3) using a scalpel. 3. Minced fragments were transferred to V-bottom falcon tubes containing a digestion mix (RPMI culture medium with 100 μg/ml Liberase™ TL (Roche) and 100 μg/ml DNase I (Roche)). 4. Tissues were then dissociated with a combination of enzymatic digestion (37°C for 30min, water bath) and mechanical disruption (16G syringe needle) under continuous stirring with a combination of tiny ball-shaped and cylinder-shaped magnetic stirrers. 5. The mechanical disruption was performed 15 minutes after enzymatic digestion start by gently passing the samples through a 16G syringe needle (10x). 6. Enzymatic digestion was stopped with RPMI, containing 10% FCS followed by filtering the cell suspension through 40um strainer. 7. Nondigested tissue fragments, remaining on the strainer, were gently massaged with the syringe plunger head, to release the remaining synovial cells into filtered cell suspension. Any remaining drops of cell suspension were collected from the bottom side of the strainer with a fresh pipette tip and added to the filtered cell suspension. Additional washes were introduced to minimize cell loss. 8. Red blood cells in dissociated synovial cell pellets were lysed using Red Cell Lysis buffer (Roche), according to the manufacturer's instructions and cells were washed with PBS. All washing steps and volumes of reaction mixes were optimized to minimize cell loss throughout the protocol. Single cell 3´protocol (10x Genomics), v3.0/v3.1

text: adult

text: arthritis

text: Homo sapiens

text: Homo sapiens

text: synovial membrane

text: Homo sapiens

text: female

characteristic: title


text: BioMed X GmbH

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: human

characteristic: description

text: adult

text: arthritis

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: synovial membrane

text: Homo sapiens

text: male


text: UGent

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: CLB-GA

characteristic: cell line

text: neuroblast (sensu Vertebrata)

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Cell lines were harvested, centrifuged and stored at -80°C. Cells were grown in RPMI1640 medium supplemented with 10% foetal bovine serum (FBS), 2 mM L-Glutamine and 100 IU/ml penicillin/streptavidin at 37°C in a 5% CO2 humid atmosphere Cells were seeded in 6-well tissue culture plates 24 hours prior to transfection. 100nM of siRNA non-targeting control (siRNA NTC, D-001810-10-05) or siRNA SOX11 (L-017377-01-0005, Dharmacon) were transiently transfected using DharmaFect 2 (Thermo Fisher Scientific) according to the manufacturer's guidelines. For nucleofection, cells were nucleofected with 100 nM of the above described siRNA NTC and siRNA SOX11 using the Neon Transfection System (Thermo Fisher Scientific) and subsequently seeded in 6-well or T25 tissue culture plates. Total RNA was extracted using miRNeasy kit (Qiagen) according to manufacturer's instructions, including on-column DNase treatment, and concentration was determined with the Nanodrop (Thermo Scientific). CLB-GA samples were prepared using the TruSeq Stranded mRNA Sample Prep Kit from Illumina. And NGP samples were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit from Illumina.

text: neuroblastoma

text: wild type genotype

characteristic: genotype

text: bone marrow

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: scrambled siRNA

characteristic: rna interference

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UGent

characteristic: INSDC first public

text: public

text: ArrayExpress

text: CLB-GA

text: neuroblast (sensu Vertebrata)

text: human

text: neuroblastoma

characteristic: disease

text: wild type genotype

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

characteristic: organism part

text: SOX11 knockdown by siRNA

text: Homo sapiens


characteristic: External Id

text: UGent

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: neuroblast (sensu Vertebrata)

text: human

text: neuroblastoma

text: wild type genotype

text: bone marrow

text: Homo sapiens

text: Homo sapiens

text: brain

text: SOX11 knockdown by siRNA

text: Homo sapiens

characteristic: title


text: UGent

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: NGP

characteristic: cell line

text: neuroblast (sensu Vertebrata)

characteristic: cell type

text: human

characteristic: description

text: neuroblastoma

text: wild type genotype

text: lung

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: brain

text: scrambled siRNA

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UGent

text: public

text: ArrayExpress

text: NGP

text: neuroblast (sensu Vertebrata)

text: human

characteristic: common name

text: neuroblastoma

text: wild type genotype

characteristic: genotype

text: lung

characteristic: metastatic site

text: Homo sapiens

text: Homo sapiens

text: brain

text: SOX11 knockdown by siRNA

characteristic: rna interference

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of West London

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: collection date

text: Armenian Clinical isolate

text: Armenia

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

characteristic: isolate

text: urine

characteristic: isolation source

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Armenian Clinical isolate


E-MTAB-11892:SH-EP_C11 DOX 9h rep1

text: UGent

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11892:SH-EP_C11 DOX 9h rep1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: SHEP

characteristic: cell line

text: neuroblast (sensu Vertebrata)

characteristic: cell type

text: human

text: Protocols: Cell lines were harvested, centrifuged and stored at -80°C. Cells were grown in RPMI1640 medium supplemented with 10% foetal bovine serum, 2 mM L-Glutamine and 100 IU/ml penicillin/streptavidin at 37°C in a 5% CO2 humid atmosphere, supplemented with 10% tetracyclin-free FBS to avoid leakage. For SOX11 inducible overexpression, the OriGene vector SC303275 containing the cDNA of SOX11 was amplified by PCR and the obtained fragment was gel purified and ligated into the opened NdeI site of response vector pLVX-TRE2G-Zsgreen1 (Takara, cat#631353) producing pLVX-TRE3G-Zsgreen1-IRES-hSOX11. The constructed plasmid was verified by restriction digest and sequenced by Sanger DNA sequencing (GATC). Lenti-X 293T Cells (Takara, cat#632180) were transfected with the regulator vector pLVX-pEF1a-Tet3G (cat#631353 and Lenti-X Packaging Single Shots (VSV-G) (cat#631275) according to the manufacturer’s instructions. The supernatant containing the lenti-virus was collected, filtered through a 0.45 µm filter and concentrated using PEG-IT. SH-EP cells were infected with the concentrated virus and 48 hours of incubation thereafter, the transduced cells were selected using 500 µg/ml G418. Individual clones were obtained by limiting dilution. After clonal expansion, the TET protein expression in each clone was checked by immunoblotting using TetR monoclonal antibody (Clone 9G9) (Clontech, cat#631131). In addition, induction of each expressing clone was tested after transduction with the pLVX-TRE3G-Luc control vector. Selected clones were transduced with lentivirus produced as described above from vector pLVX-TRE3G-Zsgreen1-IRES-hSOX11 and subsequently selected with 1 µg/ml puromycin. Each clone was treated with 0.1 ug/ml Doxycycline for 9h for induction of SOX11 expression or left unaffected in case of the control Total RNA was extracted using miRNeasy kit (Qiagen) according to manufacturer's instructions, including on-column DNase treatment, and concentration was determined with the Nanodrop (Thermo Scientific). 250 ng of RNA isolated from cells was used as input for library preparation with the TruSeq Stranded mRNA Sample Prep Kit from Illumina, followed by quality control on the fragment analyser and qPCR quantificaiton of the libraries using the Illumina Kapa Library quantification kit (Ligtcycler 480 qPCR mix Kapa Code KK4854)

characteristic: description

text: neuroblastoma

characteristic: disease

text: doxycycline-inducible SOX11 expression clone C11

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: SOX11 overexpression

characteristic: phenotype

text: Homo sapiens

text: doxycycline; 0.1 microgram per milliliter

characteristic: stimulus

text: SH-EP_C11 DOX 9h rep1

characteristic: title


E-MTAB-11892:SH-EP_C11 DOX 9h rep2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UGent

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-11892:SH-EP_C11 DOX 9h rep2

text: ArrayExpress

text: SHEP

text: neuroblast (sensu Vertebrata)

text: human

characteristic: common name

text: neuroblastoma

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: SOX11 overexpression

text: Homo sapiens

text: SH-EP_C11 DOX 9h rep2


E-MTAB-11892:SH-EP_C11 UT 9h rep1

text: UGent

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11892:SH-EP_C11 UT 9h rep1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: SHEP

characteristic: cell line

text: neuroblast (sensu Vertebrata)

text: human

text: neuroblastoma

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: wild type

text: Homo sapiens

text: SH-EP_C11 UT 9h rep1


E-MTAB-11892:SH-EP_C11 UT 9h rep2

text: UGent

text: public

text: E-MTAB-11892:SH-EP_C11 UT 9h rep2

text: ArrayExpress

text: SHEP

text: neuroblast (sensu Vertebrata)

characteristic: cell type

text: human

characteristic: description

text: neuroblastoma

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: wild type

characteristic: phenotype

text: Homo sapiens

text: SH-EP_C11 UT 9h rep2


E-MTAB-11892:SH-EP_G2 DOX 9h rep1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UGent

text: public

text: E-MTAB-11892:SH-EP_G2 DOX 9h rep1

text: ArrayExpress

text: SHEP

text: neuroblast (sensu Vertebrata)

text: human

text: neuroblastoma

text: doxycycline-inducible SOX11 expression clone G2

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: SOX11 overexpression

text: Homo sapiens

characteristic: stimulus

text: SH-EP_G2 DOX 9h rep1

characteristic: title


E-MTAB-11892:SH-EP_G2 UT 9h rep1

text: UGent

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-11892:SH-EP_G2 UT 9h rep1

text: ArrayExpress

text: SHEP

text: neuroblast (sensu Vertebrata)

text: human

characteristic: common name

text: neuroblastoma

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: wild type

text: Homo sapiens

text: SH-EP_G2 UT 9h rep1


E-MTAB-11897:Aspartate + 1

text: public

characteristic: SRA accession

text: E-MTAB-11897:Aspartate + 1

tag: Namespace:BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Samples were collected at the early exponential growth phase (OD600 ~1) and treated with RNAprotect Bacteria Reagent (QIAGEN, Germany) according to the manufacturer instructions. C. necator H16 ΔmmsA123 ΔphaCAB colonies were used to inoculate tubes containing 5 ml of LB. Each tube was incubated at 30°C overnight with shaking at 200 rpm. Precultures were centrifugated at 8000 rpm for 3 min, washed twice and used to prepare 25 ml of culture at OD600 ~0.1 in a 250 ml baffled flask. 2%SGMM containing 4 g/l (NH4)2SO4 with and without 4 g/l aspartate was used as the culture medium. The flasks were incubated at 30°C with shaking at 200 rpm. Performed by GENEWIZ Performed by GENEWIZ

characteristic: description

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

characteristic: growth condition

text: Cupriavidus necator H16

text: Cupriavidus necator H16

text: whole organism

text: Cupriavidus necator H16

text: Aspartate + 1


E-MTAB-11897:Aspartate + 2

characteristic: ENA-CHECKLIST

text: BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham

text: public

text: E-MTAB-11897:Aspartate + 2

text: ArrayExpress

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

text: Cupriavidus necator H16

characteristic: organism

text: Cupriavidus necator H16

text: whole organism

characteristic: organism part

text: Cupriavidus necator H16

text: Aspartate + 2


E-MTAB-11897:Aspartate + 3

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-11897:Aspartate + 3

text: ArrayExpress

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

text: Cupriavidus necator H16

text: Cupriavidus necator H16

text: whole organism

text: Cupriavidus necator H16

text: Aspartate + 3


E-MTAB-11897:Aspartate - 1

characteristic: INSDC center name

text: public

text: E-MTAB-11897:Aspartate - 1

text: ArrayExpress

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

text: Cupriavidus necator H16

text: Cupriavidus necator H16

text: whole organism

text: Cupriavidus necator H16

text: Aspartate - 1

characteristic: title


E-MTAB-11897:Aspartate - 2

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11897:Aspartate - 2

text: ArrayExpress

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

text: Cupriavidus necator H16

text: Cupriavidus necator H16

text: whole organism

text: Cupriavidus necator H16

text: Aspartate - 2


E-MTAB-11897:Aspartate - 3

text: public

characteristic: INSDC status

text: E-MTAB-11897:Aspartate - 3

text: ArrayExpress

text: ΔmmsA1 ΔmmsA2 ΔmmsA3 ΔphaCAB

text: 2%SGMM containing 4 g/l (NH4)2SO4

text: Cupriavidus necator H16

text: Cupriavidus necator H16

text: whole organism

text: Cupriavidus necator H16

text: Aspartate - 3


text: University of Zurich

text: public

text: See sample_title

text: Albizia sp. Koenen 387 G

text: Albizia sp. Koenen 387 G

text: Koenen 387 (G)

characteristic: specimen voucher

text: Hybrid capture sequence reads of Albizia sp.. Voucher: Koenen 387 (G)


text: University of Zurich

text: public

characteristic: SRA accession

text: See sample_title

text: Albizia amara subsp. amara

text: Albizia amara subsp. amara

text: Semsei 3274 (PRE)

text: Hybrid capture sequence reads of Albizia amara subsp. amara. Voucher: Semsei 3274 (PRE)


text: The Francis Crick Institute

text: public

tag: Namespace:The Francis Crick Institute

characteristic: Submitter Id

characteristic: cell line

text: house mouse

characteristic: common name

text: cell_line_KP_A3Bi.het_cell line

text: KrasG12D/WT; Trp53fl/fl; R26::APOBEC3Bi/WT

characteristic: genotype

characteristic: mouse id

text: Mus musculus

text: Mus musculus

text: M

characteristic: sex

characteristic: strain

text: cell line

characteristic: tissue type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Francis Crick Institute

text: public

text: house mouse

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

text: M

text: tail


characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: cell_line_KP_A3Bi.het_Rag1KO_cell line

text: KrasG12D/WT; Trp53fl/fl; R26::APOBEC3Bi/WT; Rag1KO/KO

text: Mus musculus

text: Mus musculus

text: F

text: cell line

characteristic: title


text: The Francis Crick Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus

text: F

text: tail


text: The Francis Crick Institute

text: public

text: house mouse

characteristic: common name

text: KrasG12D/WT; Trp53fl/fl

characteristic: genotype

characteristic: mouse id

text: Mus musculus

text: Mus musculus

text: M

text: tumour

text: AdCre

characteristic: treatment


characteristic: ENA-CHECKLIST

text: The Francis Crick Institute

text: public

text: house mouse

text: KrasG12D/WT; Trp53fl/fl

text: Mus musculus

text: Mus musculus

text: M

characteristic: sex

characteristic: strain

text: tail

characteristic: tissue type

text: AdCre


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Francis Crick Institute

text: public

text: house mouse

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

text: M

text: tumour

text: AdCre


characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: M

text: tail

text: AdCre


text: The Francis Crick Institute

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Mus musculus

text: F

text: tumour

characteristic: title

text: AdCre


text: The Francis Crick Institute

text: public

text: house mouse

characteristic: common name

text: Ur_A3Bi het ; Rag1 het_tumour

characteristic: genotype

characteristic: mouse id

text: Mus musculus

text: Mus musculus

text: F

text: tumour

text: urethane

characteristic: treatment


characteristic: ENA-CHECKLIST

text: The Francis Crick Institute

text: public

text: house mouse

text: Ur_A3Bi het ; Rag1 het_tail

text: Mus musculus

text: Mus musculus

text: F

characteristic: sex

characteristic: strain

text: tail

characteristic: tissue type

text: urethane


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Francis Crick Institute

text: public

text: house mouse

text: UrA3Bi.CreER_A3Bi het ; CreER het_tumour

characteristic: description

text: Mus musculus

characteristic: organism

text: Mus musculus

text: M

text: tumour

text: urethane+tamoxifen


characteristic: External Id

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: house mouse

text: UrA3Bi.CreER_A3Bi het ; CreER het_tail

text: Mus musculus

text: Mus musculus

text: M

text: tail

text: urethane+tamoxifen


E-MTAB-11902:Sample 1

text: THE JACKSON LABORATORY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-11902:Sample 1

tag: Namespace:THE JACKSON LABORATORY

characteristic: Submitter Id

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: mesenchymal cell

characteristic: cell type

text: house mouse

text: Protocols: Naïve and tumor-bearing CD140a-EGFP mice were euthanized, and lung was harvested and digested. Lung single cell suspension was prepared and stained with primary-fluorophore conjugated antibodies. Lung CD140a+ mesenchymal cells (CD45-CD31-CD326-GFP+) were isolated by fluorescence-activated cell sorting. Cells capture, lysis and RNA extraction were performed using the 10x V3 Chromium technology. For each sample, around 12,000 cells were loaded in each lane of the 10x Chromium chip. Barcoding and library preparation were performed with the 10x Chromium system using version 3 chemistry, following the manufacturer’s protocol. cDNA was synthesized and amplified as per manufacturer’s protocol and amplified to construct an Illumina sequencing library. cDNA and libraries were checked for quality on an Agilent 4200 TapeStation, quantified by KAPA qPCR.

text: adult

characteristic: developmental stage

text: CD45-; CD31-; CD326-; GFP+

characteristic: immunophenotype

characteristic: individual

text: Mus musculus

text: Mus musculus

text: lung

characteristic: organism part

text: Mus musculus

text: female

text: Sample 1

characteristic: title


E-MTAB-11902:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: THE JACKSON LABORATORY

text: public

text: E-MTAB-11902:Sample 2

text: ArrayExpress

text: mesenchymal cell

text: house mouse

characteristic: common name

characteristic: description

text: adult

characteristic: genotype

text: CD45-; CD31-; CD326-; GFP+

text: Mus musculus

text: Mus musculus

text: lung

text: Mus musculus

text: female

characteristic: sex

text: tumor bearing

characteristic: stimulus

characteristic: strain

text: Sample 2


characteristic: External Id

text: University of Surrey

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University of Surrey

characteristic: Submitter Id

text: not provided

characteristic: collection date

text: house mouse

text: polysomal mRNA at month 3, replicate 10

characteristic: dev stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: female

text: not provided

text: hippocampus

characteristic: tissue type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Surrey

text: public

text: not provided

text: house mouse

characteristic: common name

text: total mRNA at month 3, replicate 10

text: Mus musculus

text: Mus musculus

text: female

text: not provided

text: hippocampus

characteristic: title


text: University of Surrey

text: public

text: not provided

text: house mouse

text: polysomal mRNA at month 12, replicate 13

characteristic: description

text: Mus musculus

text: Mus musculus

text: female

characteristic: sex

text: not provided

characteristic: strain

text: hippocampus


characteristic: External Id

text: University of Surrey

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: not provided

text: house mouse

text: total mRNA at month 12, replicate 13

text: Mus musculus

text: Mus musculus

text: female

text: not provided

text: hippocampus


text: University of Surrey

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: not provided

text: house mouse

text: polysomal mRNA at month 3, replicate 14

text: Mus musculus

text: Mus musculus

text: female

text: not provided

text: hippocampus


text: University of Surrey

text: public

characteristic: Submitter Id

text: not provided

characteristic: collection date

text: house mouse

text: total mRNA at month 3, replicate 14

characteristic: dev stage

text: Mus musculus

text: Mus musculus

text: female

text: not provided

text: hippocampus


characteristic: ENA-CHECKLIST

text: University of Surrey

text: public

text: not provided

text: house mouse

text: polysomal mRNA at month 20, replicate 15

text: Mus musculus

characteristic: organism

text: Mus musculus

text: female

text: not provided

text: hippocampus

characteristic: tissue type