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characteristic: organism

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text: Severe acute respiratory syndrome coronavirus 2

text: active surveillance in response to outbreak


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characteristic: collecting institution

characteristic: collection date

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tag: Namespace:e-NIOS Applications PC

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characteristic: common name

text: Protocols: Two human epithelial BC cell lines, authenticated in July 2018 by the short tandem repeat DNA profiling, were used in this study. JIMT-1 (DSMZ no.: ACC 589). Cells were cultivated at 37°C in a humidified atmosphere and 5% CO2in high-glucose (4.5 g/l) Dulbecco’s modified Eagle medium (DMEM, PAA Laboratories GmbH, Austria) supplemented with 10% fetal bovine serum (FBS, Biochrom AG, Germany), 10 μg/ml gentamicin (Sandoz, Germany) and 2 mM glutamine (PAA Laboratories GmbH). RNeasy Mini Kit (Qiagen, Germany) was used to isolate total RNA from cell pellets, using the On-column DNase digestion step with the RNAse-Free DNase Set (Qiagen). Extracted RNA quality and quantity were evaluated using NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific). BGI DNBseq library construction

characteristic: description

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text: mammary gland

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text: trastuzumab resistant

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text: ArrayExpress

text: human

text: breast ductal adenocarcinoma

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text: Homo sapiens

text: mammary gland

text: luminal A subtype

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characteristic: collecting institution

characteristic: collection date

text: Pascal Schläpfer, Rainer Gosert, Klaudia Nägele, Svenia Schmid, Karoline Leuzinger, Hans H Hirsch

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

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text: University Hospital Basel

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text: Severe acute respiratory syndrome coronavirus 2

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characteristic: GISAID Accession ID

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characteristic: collecting institution

characteristic: collection date

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characteristic: geographic location (region and locality)

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text: active surveillance not initiated by an outbreak


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characteristic: GISAID Accession ID

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text: ena-upload-cli

tag: Namespace:Clinical Bioinformatics Area

text: Servicio de Microbiologia, Hospital Universitario San Cecilio, Granada, Spain

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text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

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text: not provided

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text: AND20338, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

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text: Spain

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characteristic: organism

text: AND20340, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


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text: AND20341, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


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characteristic: ENA-FIRST-PUBLIC

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text: Spain

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characteristic: organism

text: AND20344, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


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characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

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characteristic: INSDC status

text: ena-upload-cli

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characteristic: ENA-CHECKLIST

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text: not provided

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text: Spain

text: Human

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characteristic: organism

text: AND20347, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

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characteristic: ENA-CHECKLIST

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text: not provided

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text: Spain

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characteristic: title


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characteristic: ENA-FIRST-PUBLIC

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text: ena-upload-cli

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characteristic: External Id

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characteristic: INSDC last update

text: public

text: ena-upload-cli

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text: Servicio de Microbiologia, Plataforma Provincial de Almería, Almería, Spain

characteristic: collecting institution

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text: not provided

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text: Spain

text: Human

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characteristic: title


characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

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text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

characteristic: description

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characteristic: geographic location (longitude)

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characteristic: ENA-CHECKLIST

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characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: ena-upload-cli

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characteristic: title


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characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: public

characteristic: INSDC status

text: ena-upload-cli

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text: not provided

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text: not provided

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text: not provided

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characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

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characteristic: Submitter Id

characteristic: collecting institution

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text: not provided

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text: Spain

text: Human

text: not provided

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text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20360, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

text: ena-upload-cli

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text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

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text: not provided

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characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

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text: not provided

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text: not provided

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text: not provided

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text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20362, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20363, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: active surveillance in response to outbreak

text: AND20364, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

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text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

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text: AND20365, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20366, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

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characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

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text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20367, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: Servicio de Microbiologia, Plataforma Provincial de Almería, Almería, Spain

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20368, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20369, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

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text: public

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text: ena-upload-cli

text: not provided

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text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20370, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20371, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20372, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20373, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

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text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20374, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20375, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20376, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

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text: not provided

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text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20377, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: active surveillance in response to outbreak

text: AND20378, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20379, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

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text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20380, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20381, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

text: Servicio de Microbiologia, Plataforma Provincial de Almería, Almería, Spain

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20382, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region. Sequencing supported by RELECOV consortium, funded by the European Centre for Diseases Prevention and Control (ECDC) under Grant Agreement ECDC/HERA/2021/024 ECDC.12241

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20383, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20384, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20385, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20386, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20387, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ena-upload-cli

text: not provided

characteristic: description

text: Spain

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Human

characteristic: host common name

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

text: AND20388, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-FIRST-PUBLIC

text: Clinical Bioinformatics Area

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ena-upload-cli

text: not provided

text: Spain

text: Human

text: not provided

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: not provided

characteristic: isolate

characteristic: sample capture status

text: AND20389, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Clinical Bioinformatics Area

characteristic: INSDC last update

text: public

text: ena-upload-cli

characteristic: Submitter Id

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Spain

text: Human

text: not provided

text: Homo Sapiens

text: not provided

text: not provided

text: not provided

characteristic: organism

text: AND20390, Severe acute respiratory syndrome coronavirus 2, WGS, Andalusia Region

characteristic: title