E-MTAB-12238:Sample 448

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 448

text: Repli G

characteristic: WGA

text: ArrayExpress

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 448


E-MTAB-12238:Sample 449

status: PUBLIC

tag: attribute

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 449

tag: Namespace:Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: hematopoietic multipotent progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 449

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 91

characteristic: xenograft


E-MTAB-12238:Sample 45

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 45

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 45

text: 1:10 human CD45 positive out of mouse bone marrow 83


E-MTAB-12238:Sample 450

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 450

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 450


E-MTAB-12238:Sample 451

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 451

text: Repli G

characteristic: WGA

text: ArrayExpress

text: B cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 451

characteristic: title


E-MTAB-12238:Sample 452

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 452

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: B cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 452


E-MTAB-12238:Sample 453

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 453

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 453

text: human CD45 positive out of mouse bone marrow 80

characteristic: xenograft


E-MTAB-12238:Sample 454

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 454

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 454

characteristic: title


E-MTAB-12238:Sample 455

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 455

text: Repli G

characteristic: WGA

text: ArrayExpress

text: B cell

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 455

text: 1:10 human CD45 positive out of mouse bone marrow 80


E-MTAB-12238:Sample 456

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 456

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: B cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 456

characteristic: xenograft


E-MTAB-12238:Sample 457

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 457

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: DNA Purification from PB

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 457

characteristic: title


E-MTAB-12238:Sample 458

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 458

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: DNA Purification from PB

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 458


E-MTAB-12238:Sample 459

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 459

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 459


E-MTAB-12238:Sample 46

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 46

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 46

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 460

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 460

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 460


E-MTAB-12238:Sample 461

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 461

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 461


E-MTAB-12238:Sample 462

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 462

text: Repli G

characteristic: WGA

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 462

characteristic: title


E-MTAB-12238:Sample 463

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 463

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 463


E-MTAB-12238:Sample 464

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 464

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 464


E-MTAB-12238:Sample 465

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 465

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 465

characteristic: title


E-MTAB-12238:Sample 466

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 466

text: Repli G

characteristic: WGA

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 466


E-MTAB-12238:Sample 467

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 467

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: B cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 467


E-MTAB-12238:Sample 468

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 468

text: Repli G

characteristic: WGA

text: ArrayExpress

text: B cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 468

characteristic: title


E-MTAB-12238:Sample 469

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 469

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD45 positive

characteristic: subclone

text: Sample 469

text: human CD45 positive out of mouse bone marrow 7

characteristic: xenograft


E-MTAB-12238:Sample 47

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 47

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 positive ; CD34 positive

characteristic: time

text: Sample 47


E-MTAB-12238:Sample 470

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 470

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: Sample 470

characteristic: title


E-MTAB-12238:Sample 471

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 471

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 471

text: human CD45 positive out of mouse bone marrow 78

characteristic: xenograft


E-MTAB-12238:Sample 472

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 472

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: time

text: Sample 472


E-MTAB-12238:Sample 473

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 473

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 473

characteristic: title


E-MTAB-12238:Sample 474

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 474

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 474


E-MTAB-12238:Sample 475

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 475

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 475


E-MTAB-12238:Sample 476

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 476

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 476

characteristic: title


E-MTAB-12238:Sample 477

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 477

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 positive

characteristic: subclone

text: Sample 477

text: 1:10 human CD45 positive out of mouse bone marrow 7

characteristic: xenograft


E-MTAB-12238:Sample 478

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 478

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: time

text: Sample 478


E-MTAB-12238:Sample 479

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 479

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: DNA Purification from PB

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 479

characteristic: title


E-MTAB-12238:Sample 48

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 48

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 48


E-MTAB-12238:Sample 480

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 480

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: DNA Purification from PB

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 480


E-MTAB-12238:Sample 481

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 481

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 481

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 78

characteristic: xenograft


E-MTAB-12238:Sample 482

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 482

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 482


E-MTAB-12238:Sample 483

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 483

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 483


E-MTAB-12238:Sample 484

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 484

text: Repli G

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 484

characteristic: title


E-MTAB-12238:Sample 485

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 485

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 485


E-MTAB-12238:Sample 486

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 486

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 486


E-MTAB-12238:Sample 487

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 487

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: Sample 487

characteristic: title

text: human CD45 positive out of mouse bone marrow 9

characteristic: xenograft


E-MTAB-12238:Sample 488

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 488

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 488


E-MTAB-12238:Sample 489

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 489

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: time

text: Sample 489

text: human CD45 positive out of mouse bone marrow 79


E-MTAB-12238:Sample 49

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 49

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 49

characteristic: title


E-MTAB-12238:Sample 490

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 490

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 490

characteristic: xenograft


E-MTAB-12238:Sample 491

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 491

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: time

text: Sample 491


E-MTAB-12238:Sample 492

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 492

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: Sample 492

characteristic: title


E-MTAB-12238:Sample 493

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 493

text: Repli G

text: ArrayExpress

text: hematopoietic multipotent progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 493


E-MTAB-12238:Sample 494

text: Department of Molecular Cell Biology, Weizmann Institute of Science

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 494

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 494


E-MTAB-12238:Sample 495

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 495

text: Repli G

text: ArrayExpress

characteristic: cell count

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: Sample 495

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 9

characteristic: xenograft


E-MTAB-12238:Sample 496

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 496

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 496


E-MTAB-12238:Sample 497

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 497

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 497

text: 1:10 human CD45 positive out of mouse bone marrow 79


E-MTAB-12238:Sample 498

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 498

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 498

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 499

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 499

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 499


E-MTAB-12238:Sample 5

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 5

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 5


E-MTAB-12238:Sample 50

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 50

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 50

characteristic: title


E-MTAB-12238:Sample 500

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 500

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 500


E-MTAB-12238:Sample 51

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 51

text: Repli G

text: ArrayExpress

characteristic: cell count

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: time

text: Sample 51

text: human CD45 positive out of mouse bone marrow 22

characteristic: xenograft


E-MTAB-12238:Sample 52

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 52

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 52

characteristic: title


E-MTAB-12238:Sample 53

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 53

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 53

text: human CD45 positive out of mouse bone marrow 84


E-MTAB-12238:Sample 54

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 54

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: time

text: Sample 54

characteristic: xenograft


E-MTAB-12238:Sample 55

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 55

text: Repli G

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 55

characteristic: title


E-MTAB-12238:Sample 56

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 56

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 56


E-MTAB-12238:Sample 57

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 57

text: Repli G

text: ArrayExpress

characteristic: cell count

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: time

text: Sample 57

text: 1:10 human CD45 positive out of mouse bone marrow 22

characteristic: xenograft


E-MTAB-12238:Sample 58

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 58

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 58

characteristic: title


E-MTAB-12238:Sample 59

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 59

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 59

text: 1:10 human CD45 positive out of mouse bone marrow 84


E-MTAB-12238:Sample 6

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 6

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 6


E-MTAB-12238:Sample 60

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 60

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 60

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 61

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 61

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 61


E-MTAB-12238:Sample 62

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 62

text: Repli G

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 62


E-MTAB-12238:Sample 63

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 63

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD45 positive

text: Sample 63

characteristic: title

text: human CD45 positive out of mouse bone marrow 25

characteristic: xenograft


E-MTAB-12238:Sample 64

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 64

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 64


E-MTAB-12238:Sample 65

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 65

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: time

text: Sample 65

text: human CD45 positive out of mouse bone marrow 86


E-MTAB-12238:Sample 66

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 66

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: Sample 66

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 67

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 67

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 67


E-MTAB-12238:Sample 68

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 68

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 68


E-MTAB-12238:Sample 69

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 69

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 69

characteristic: title


E-MTAB-12238:Sample 7

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 7

text: Repli G

text: ArrayExpress

characteristic: cell count

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

text: Sample 7

text: human CD45 positive out of mouse bone marrow 11

characteristic: xenograft


E-MTAB-12238:Sample 70

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 70

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 70


E-MTAB-12238:Sample 71

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 71

text: Repli G

text: ArrayExpress

characteristic: cell count

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 positive

characteristic: subclone

text: Sample 71

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 25


E-MTAB-12238:Sample 72

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 72

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 72

characteristic: xenograft


E-MTAB-12238:Sample 73

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 73

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 73

text: 1:10 human CD45 positive out of mouse bone marrow 86


E-MTAB-12238:Sample 74

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 74

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 74

characteristic: title


E-MTAB-12238:Sample 75

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 75

text: Repli G

text: ArrayExpress

text: hematopoietic multipotent progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 75


E-MTAB-12238:Sample 76

text: Department of Molecular Cell Biology, Weizmann Institute of Science

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 76

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 76


E-MTAB-12238:Sample 77

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 77

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 77

characteristic: title

text: human CD45 positive out of mouse bone marrow 72

characteristic: xenograft


E-MTAB-12238:Sample 78

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 78

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 78


E-MTAB-12238:Sample 79

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 79

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

characteristic: time

text: Sample 79

text: human CD45 positive out of mouse bone marrow 87


E-MTAB-12238:Sample 8

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 8

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 8

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 80

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 80

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 80


E-MTAB-12238:Sample 81

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 81

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 81


E-MTAB-12238:Sample 82

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 82

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 82

characteristic: title


E-MTAB-12238:Sample 83

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 83

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 83

text: 1:10 human CD45 positive out of mouse bone marrow 72

characteristic: xenograft


E-MTAB-12238:Sample 84

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 84

text: Repli G

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 84


E-MTAB-12238:Sample 85

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 85

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 85

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 87


E-MTAB-12238:Sample 86

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 86

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: date

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 86

characteristic: xenograft


E-MTAB-12238:Sample 87

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 87

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 87