characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981120 of study SRP384936.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridiaceae bacterium

text: shot-gun sequecing

text: uncultured Clostridiaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981120_bin.6_metawrap_v1.3_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

unit: DD

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981120_bin.7_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Oscillibacter sp.

text: shot-gun sequecing

text: uncultured Oscillibacter sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Oscillospiraceae;g__Oscillibacter;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981120_bin.8_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981121 of study SRP384936.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridium sp.

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.11_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

text: shot-gun sequecing

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981121_bin.13_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.17_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981121_bin.21_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Anaerotruncus sp.

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Anaerotruncus sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Anaerotruncus;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.24_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured bacterium

text: shot-gun sequecing

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981121_bin.28_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.29_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981121_bin.30_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.4_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981121 of study SRP384936.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981121_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Eubacterium sp.

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Eubacterium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981121_bin.9_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981122 of study SRP384936.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.11_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Alistipes finegoldii

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Alistipes finegoldii

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__Alistipes finegoldii

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981122_bin.12_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Tenericutes bacterium

text: shot-gun sequecing

text: uncultured Tenericutes bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.17_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981122_bin.2_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Hungatella sp.

text: shot-gun sequecing

text: uncultured Hungatella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.24_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981122_bin.27_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Bacteroidales bacterium

text: shot-gun sequecing

text: uncultured Bacteroidales bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.30_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981122_bin.32_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981122 of study SRP384936.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

text: shot-gun sequecing

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.4_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Bacteroides acidifaciens

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Bacteroides acidifaciens

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides;s__Bacteroides acidifaciens

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981122_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Alistipes sp.

text: shot-gun sequecing

text: uncultured Alistipes sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981122_bin.8_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981123 of study SRP384936.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Candidatus Saccharibacteria bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridiaceae bacterium

text: shot-gun sequecing

text: uncultured Clostridiaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.11_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.12_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.13_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.15_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

text: shot-gun sequecing

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Catabacteriaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.16_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.18_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured bacterium

text: shot-gun sequecing

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.20_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981123 of study SRP384936.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.21_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Bacillales bacterium

text: shot-gun sequecing

text: uncultured Bacillales bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.24_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.25_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

text: shot-gun sequecing

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.27_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.4_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured bacterium

text: shot-gun sequecing

text: uncultured bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981123_bin.5_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Hungateiclostridiaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981123_bin.7_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981124 of study SRP384936.

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Alphaproteobacteria bacterium

text: shot-gun sequecing

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.1_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Muribaculaceae bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Muribaculaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981124_bin.13_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Candidatus Saccharibacteria bacterium

text: shot-gun sequecing

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.14_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: Parabacteroides goldsteinii

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Parabacteroides goldsteinii

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides;s__Parabacteroides goldsteinii

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981124_bin.17_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: shot-gun sequecing

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.22_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981124_bin.25_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Lactobacillus gasseri

text: shot-gun sequecing

text: Lactobacillus gasseri

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus gasseri

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.26_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR19981124 of study SRP384936.

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Firmicutes bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured Firmicutes bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981124_bin.28_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

text: shot-gun sequecing

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.4_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

text: not provided

characteristic: common name

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: not provided

text: not provided

text: not provided

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: organism

text: shot-gun sequecing

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR19981124_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

text: not provided

characteristic: collection date

text: CheckM

characteristic: description

text: feces

text: not provided

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: geographic location (latitude)

text: not provided

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Roseburia sp.

text: shot-gun sequecing

text: uncultured Roseburia sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR19981124_bin.8_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OSLO

text: public

tag: Namespace:UNIVERSITY OF OSLO

text: Norway

text: mycobacterium tuberculosis

characteristic: host scientific name

characteristic: isolate

text: genome

text: Mycobacterium tuberculosis

characteristic: organism

text: Mycobacterium tuberculosis


text: NIPH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ELIXIR-Norway

characteristic: broker name

text: S. pyogenes

characteristic: common name

text: GAS routine sample

text: Norway

text: not provided

characteristic: host health state

text: Homo sapiens

text: joint fluid

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: NIPH

text: public

text: ELIXIR-Norway

text: S. pyogenes

text: GAS routine sample

characteristic: description

text: Norway

characteristic: geographic location (country and/or sea)

text: not provided

text: Homo sapiens

text: blood

text: Streptococcus pyogenes

text: Streptococcus pyogenes

characteristic: title


E-MTAB-10502_2:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 10

tag: Namespace:The Hebrew University of Jerusalem

text: ArrayExpress

text: Protocols: centrifuged for 5 min at 4°C, 4500g, resuspended in 50 µl TE (10 mM Tris-HCl pH 7.5, 1 mM EDTA) and mixed with 5 ul of 9 mg/ml lysozyme prior to freezing the sample at liquid nitrogen and storing it at -80°C. Bacterial strains used in this study are listed in Table S1. The gene expression levels of two strains were compared: (1) a strain in which the sRNA is induced for a short time, from the endogenous chromosomal locus (in the study of RyhB). (2) A control strain in which the sRNA is not induced, a deletion strain of the sRNA gene, a ryhB deletion strain. Bacteria were routinely grown at 37°C in LB rich medium. In all experiments, single colonies were suspended in liquid medium and grown over night. The next day the cultures were diluted 1:100 in fresh medium and grown to an OD600 of 0.5. Where indicated, the iron chelator 2,2’-Dipyridy (200 µM final; DIP) was added to strain (1) or (2) the culture for the last 30 minutes of growth. Bacterial strains used in this study are listed in Table S1. The gene expression levels of two strains were compared: (1) a strain in which the sRNA is induced for a short time, from the endogenous chromosomal locus (in the study of RyhB). (2) A control strain in which the sRNA is not induced, a deletion strain of the sRNA gene, a ryhB deletion strain. Bacteria were routinely grown at 37°C in LB rich medium. In all experiments, single colonies were suspended in liquid medium and grown over night. The next day the cultures were diluted 1:100 in fresh medium and grown to an OD600 of 0.5. Where indicated, the iron chelator 2,2’-Dipyridy (200 µM final; DIP) was added to strain (1) or (2) the culture for the last 30 minutes of growth. Frozen samples were subjected to two cycles of thawing at 37°C and refreezing in liquid nitrogen. Next, the samples were re-suspended thoroughly to homogenization with 1 mL TriReagent (pre-warmed to room temperature) and incubated for 5 min at room temperature. Two hundred microliters of chloroform were added, and the tubes content was mixed by inversing the tubes for 15 s. The samples were incubated for 10 min at room temperature, centrifuged (17,000g, 10 min at 4C) and the upper phase was collected and transferred into new Eppendorf tubes. For RNA precipitation, 500 l isopropanol were added, the tube contents were mixed thoroughly by inversion of the tubes and incubated for 10 min at room temperature. The tubes were centrifuged (17,000g,15 min at 4°C) and the supernatant was discarded. The pellets were washed twice by addition of 1 mL of freshly made 75% (vol/vol) ethanol, followed by centrifugation (17,000g, 5 min at 4°C) and removal of the supernatant. Pellets were air dried by at room temperature, and then re-suspended in 300 L nuclease free water and stored at -20°C. The RNA concentration was measured using Nanodrop (ThermoFisher Scientific). The experiments were performed in triplicates for each strain. RNA-seq libraries were constructed according to the RNAtag-Seq protocol {Shishkin, 2015 #973} with several modifications, to enable capturing of short RNA fragments {Melamed, 2018 #1252}. The libraries were single-end sequenced with read length of 92 bases on NexstSeq500 Sequencer (Illumina).

text: log phase

characteristic: developmental stage

text: deletion of ryhB

characteristic: genotype

text: LB broth

characteristic: growth condition

text: Escherichia coli str. K-12 substr. MG1655

text: whole organism

characteristic: organism part

characteristic: strain

text: Sample 10


E-MTAB-10502_2:Sample 11

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10502_2:Sample 11

text: ArrayExpress

text: log phase

text: deletion of ryhB

text: LB broth

characteristic: organism

text: whole organism

text: Sample 11


E-MTAB-10502_2:Sample 12

text: The Hebrew University of Jerusalem

text: public

characteristic: SRA accession

text: E-MTAB-10502_2:Sample 12

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: description

text: log phase

text: deletion of ryhB

text: LB broth

text: whole organism

text: Sample 12


E-MTAB-10502_2:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 9

text: ArrayExpress

text: log phase

text: deletion of ryhB

text: LB broth

text: whole organism

text: Sample 9

characteristic: title


E-MTAB-10502_2:Sample 4

characteristic: ENA-LAST-UPDATE

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 4

text: ArrayExpress

text: log phase

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: LB broth

text: whole organism

text: Sample 4


E-MTAB-10502_2:Sample 5

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 5

text: ArrayExpress

text: log phase

text: wild type genotype

text: LB broth

characteristic: growth condition

text: whole organism

characteristic: organism part

characteristic: strain

text: Sample 5


E-MTAB-10502_2:Sample 6

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-10502_2:Sample 6

text: ArrayExpress

text: log phase

text: deletion of ryhB

text: LB broth

text: whole organism

text: Sample 6


E-MTAB-10502_2:Sample 7

text: The Hebrew University of Jerusalem

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10502_2:Sample 7

text: ArrayExpress

text: log phase

text: deletion of ryhB

text: LB broth

characteristic: organism

text: whole organism

text: Sample 7


E-MTAB-10502_2:Sample 8

text: The Hebrew University of Jerusalem

text: public

characteristic: SRA accession

text: E-MTAB-10502_2:Sample 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: log phase

text: deletion of ryhB

text: LB broth

text: whole organism

text: Sample 8


E-MTAB-10502_2:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 3

text: ArrayExpress

characteristic: description

text: log phase

text: wild type genotype

text: LB broth

text: whole organism

text: Sample 3


E-MTAB-10502_2:Sample 1

characteristic: ENA-LAST-UPDATE

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 1

text: ArrayExpress

text: log phase

characteristic: developmental stage

text: wild type genotype

text: LB broth

text: whole organism

text: Sample 1

characteristic: title


E-MTAB-10502_2:Sample 13

text: The Hebrew University of Jerusalem

text: public

text: E-MTAB-10502_2:Sample 13

text: ArrayExpress

text: log phase

text: wild type genotype

characteristic: genotype

text: LB broth

characteristic: growth condition

text: whole organism

characteristic: organism part

text: Sample 13


E-MTAB-10502_2:Sample 2

characteristic: External Id

text: The Hebrew University of Jerusalem

characteristic: INSDC center name

text: public

text: E-MTAB-10502_2:Sample 2

text: ArrayExpress

text: log phase

text: wild type genotype

text: LB broth

text: whole organism

characteristic: strain

text: Sample 2


text: IAU CM

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:IAU CM

text: semiarid

characteristic: broad-scale environmental context

characteristic: collection date

text: human

characteristic: common name

text: blood

text: blood

characteristic: environmental medium

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: water scarcity

characteristic: local environmental context

text: Homo sapiens

text: Epilepsy

characteristic: project name

text: Homo sapiens

text: WES


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UNIVERSITAT DE BARCELONA

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITAT DE BARCELONA

characteristic: Submitter Id

text: Audubon's Shearwater

characteristic: description

text: USA

text: near Oregon Inlet

characteristic: geographic location (region and locality)

text: Puffinus loyemilleri

characteristic: organism

text: Puffinus loyemilleri

characteristic: title


characteristic: External Id

text: UNIVERSITAT DE BARCELONA

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Manx Shearwater

text: Iceland

text: Heimaey Island

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Barolo Shearwater

characteristic: common name

text: Spain

characteristic: geographic location (country and/or sea)

text: Lanzarote, Canary Islands

text: Puffinus baroli

text: Puffinus baroli


text: UNIVERSITAT DE BARCELONA

text: public

text: Barolo Shearwater

text: Portugal

text: Selvagems Grande

text: Puffinus baroli

text: Puffinus baroli


text: UNIVERSITAT DE BARCELONA

text: public

text: Barolo Shearwater

text: Portugal

text: Madeira

text: Puffinus baroli

text: Puffinus baroli


text: UNIVERSITAT DE BARCELONA

text: public

text: Balearic Shearwater

text: Spain

text: Menorca, Balearic Islands

text: Puffinus mauretanicus

text: Puffinus mauretanicus


text: UNIVERSITAT DE BARCELONA

text: public

text: Balearic Shearwater

text: Spain

text: Sa Cella, Mallorca, Balearic Islands

text: Puffinus mauretanicus

text: Puffinus mauretanicus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: United Kingdom

text: Isle of Rum, Scotland

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: Spain

text: Tenerife, Canary Islands

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: United Kingdom

text: Copeland Islands, Northern ireland

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: Spain

text: La Palma, Canary Islands

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: Portugal

text: Azores

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Manx Shearwater

text: Portugal

text: Madeira

text: Puffinus puffinus

text: Puffinus puffinus


text: UNIVERSITAT DE BARCELONA

text: public

text: Yelkouan Shearwater

text: Tunisia

text: Tunisia

text: Puffinus yelkouan

text: Puffinus yelkouan


text: UNIVERSITAT DE BARCELONA

text: public

text: Yelkouan Shearwater

text: Croatia

text: Croatia

text: Puffinus yelkouan

text: Puffinus yelkouan


text: United Kingdom Health Security Agency

text: public

tag: Namespace:United Kingdom Health Security Agency

characteristic: collection date

text: UK ceftriaxone resistant Neisseria gonorrhoeae isolate

text: United Kingdom

text: restricted access

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

characteristic: isolate

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: United Kingdom Health Security Agency

text: public

characteristic: SRA accession

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 2


text: United Kingdom Health Security Agency

text: public

characteristic: Submitter Id

characteristic: description

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

characteristic: organism

text: Neisseria gonorrhoeae

text: Case 3


characteristic: External Id

text: United Kingdom Health Security Agency

characteristic: INSDC center name

text: public

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 4

characteristic: title


text: United Kingdom Health Security Agency

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 5


text: United Kingdom Health Security Agency

text: public

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 6


text: United Kingdom Health Security Agency

text: public

text: United Kingdom

text: restricted access

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 7


characteristic: ENA-CHECKLIST

text: United Kingdom Health Security Agency

text: public

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

characteristic: isolate

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 8


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: United Kingdom Health Security Agency

text: public

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 9


text: United Kingdom Health Security Agency

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: United Kingdom

text: restricted access

text: Homo Sapiens

text: not provided

text: Human genital

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Case 10


text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: UNIVERSITY OF OXFORD

characteristic: barcoding center

text: COPO

characteristic: broker name

text: Campbell

text: CCAP

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Scotland | Scottish Marine Institute | SAMS | CCAP

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: Campbell

text: CCAP

text: vegetative cell

characteristic: lifestage

text: Micrasterias rotata

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: United Kingdom | Scotland | Argyll | Garden pond Kenmore Cottage

characteristic: original collection location

text: DTOL

characteristic: project name

text: WATER SAMPLE BY HAND

characteristic: sample collection device or method

text: Micrasterias rotata

characteristic: specimen voucher url

text: 54a28673-439b-4a18-add0-b8cd20cafbb7-DTOL-specimen

characteristic: tolid

characteristic: voucher institution url

type: derived from


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: UNIVERSITY OF OXFORD

characteristic: barcoding center

text: COPO

text: George

text: CCAP

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Freshwater

text: George

text: CCAP

text: vegetative cell

text: Coleochaete scutata

text: WHOLE ORGANISM

text: United Kingdom | England | Coe Fen

text: DTOL

text: WATER SAMPLE BY HAND

text: Coleochaete scutata

text: da61cbac-1e52-417d-8de6-861d6e3441d4-DTOL-specimen

characteristic: title


text: University of Oxford

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: UNIVERSITY OF OXFORD

text: COPO

characteristic: broker name

text: Jaworski

text: NOT PROVIDED

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Freshwater

characteristic: habitat

text: Jaworski

text: NOT PROVIDED

text: vegetative cell

characteristic: lifestage

text: Aulacoseira ambigua

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: United Kingdom | England | Cambridge | Garden pool Shelford

characteristic: original collection location

text: DTOL

characteristic: project name

text: NOT PROVIDED

characteristic: sample collection device or method

text: Aulacoseira ambigua

characteristic: specimen voucher url

text: 478d4122-29a9-42bd-abd4-30b9297672a1-DTOL-specimen

characteristic: tolid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: UNIVERSITY OF OXFORD

characteristic: barcoding center

text: COPO

text: Medlin

text: NOT PROVIDED

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Marine

text: Medlin

text: NOT PROVIDED

text: vegetative cell

text: Entomoneis alata

text: WHOLE ORGANISM

text: United Kingdom | England | Cumbria | Whitehaven

text: DTOL

text: NOT PROVIDED

text: Entomoneis alata

text: 17ae7553-eaaa-4c57-a1d2-81af0ad7c6f2-DTOL-specimen

characteristic: voucher institution url


text: University of Oxford

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: UNIVERSITY OF OXFORD

text: COPO

characteristic: broker name

text: Jowett

text: NOT PROVIDED

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Marine

characteristic: habitat

text: Jowett and CCAP

text: CCAP

text: vegetative cell

characteristic: lifestage

text: Marsupiomonas pelliculata

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: United Kingdom | England | Cornwall | Salt marsh Tamar Estuary

characteristic: original collection location

text: DTOL

characteristic: project name

text: NOT PROVIDED

characteristic: sample collection device or method

text: Marsupiomonas pelliculata

characteristic: specimen voucher url

text: 14e1b847-53dc-4946-a0c9-38a1b8d2d66e-DTOL-specimen

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Oxford

characteristic: GAL

text: NOT PROVIDED

text: EarlhamInstitute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: UNIVERSITY OF OXFORD

characteristic: barcoding center

text: COPO

text: Pringsheim

text: CCAP

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Freshwater

text: Pringsheim and CCAP

text: CCAP

text: vegetative cell

text: Microglena monadina

text: WHOLE ORGANISM

text: United Kingdom | England | manchester | Sample of chiefly Haematococcus

text: DTOL

text: NOT PROVIDED

text: Microglena monadina

text: 20180c79-e782-4108-8254-976effec005b-DTOL-specimen

characteristic: tolid


text: University of Oxford

text: NOT PROVIDED

text: EarlhamInstitute

text: public

text: UNIVERSITY OF OXFORD

text: COPO

characteristic: broker name

text: Turner

text: NOT PROVIDED

characteristic: collecting institution

text: United Kingdom

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: geographic location (region and locality)

text: Marine

characteristic: habitat

text: Turner and CCAP

text: CCAP

text: vegetative cell

characteristic: lifestage

text: Chloroidium ellipsoideum

characteristic: organism

text: WHOLE ORGANISM

characteristic: organism part

characteristic: original collection date

text: United Kingdom | Scotland | South of the Hebrides | Atlantic Ocean

characteristic: original collection location

text: DTOL

characteristic: project name

text: NOT PROVIDED

characteristic: sample collection device or method

text: Chloroidium ellipsoideum

characteristic: specimen voucher url

text: e3f157d8-2b7d-48c8-ad6a-7ac599d13247-DTOL-specimen

characteristic: voucher institution url