characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243806_bin.12_metawrap_v1.3_MAG


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243806 of study SRP362674.

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

unit: DD

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridium sp.

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Clostridium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243806_bin.14_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243806_bin.20_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Eubacterium sp.

characteristic: organism

text: uncultured Eubacterium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243806_bin.21_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__;c__;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243806_bin.22_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Porphyromonadaceae bacterium

characteristic: organism

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243806_bin.26_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Clostridia bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__;f__;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243806_bin.32_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Acutalibacter;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243806_bin.4_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243806 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243806_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Clostridia bacterium

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Clostridia bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243806_bin.9_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243808 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Hungatella sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Hungatella sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243808_bin.14_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Eubacterium sp.

characteristic: organism

text: uncultured Eubacterium sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Eubacteriaceae;g__Eubacterium;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243808_bin.21_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243811 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243811_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243811_bin.19_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243811_bin.27_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Dorea sp.

characteristic: organism

text: uncultured Dorea sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Dorea;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243811_bin.30_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Dorea sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Dorea sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243811_bin.31_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243811_bin.34_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: not provided

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Muribaculaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Muribaculaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243811_bin.46_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: EMG

characteristic: INSDC center name

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characteristic: INSDC last update

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: not provided

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

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text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243811_bin.54_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

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text: not provided

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text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

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text: CheckM

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characteristic: contamination score

text: feces

characteristic: environmental medium

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characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

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characteristic: local environmental context

text: mouse gut metagenome

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text: uncultured Lachnospiraceae bacterium

characteristic: project name

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text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243811_bin.6_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: EMG

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characteristic: SRA accession

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text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

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text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lactobacillus sp.

characteristic: organism

text: uncultured Lactobacillus sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus;s__Lactobacillus reuteri

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243811_bin.8_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: metaSPAdes v3.15.3

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243812 of study SRP362674.

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnospiraceae bacterium

characteristic: project name

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text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

characteristic: taxonomic classification

text: multi-marker approach

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text: Metagenome-assembled genome: SRR18243812_bin.1_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: EMG

characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

characteristic: assembly quality

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characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Hungatella sp.

characteristic: organism

text: uncultured Hungatella sp.

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hungatella;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243812_bin.17_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

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text: metaSPAdes v3.15.3

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

text: metawrap v1.3

text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Porphyromonadaceae bacterium

characteristic: project name

characteristic: sample derived from

text: uncultured Porphyromonadaceae bacterium

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Porphyromonadaceae;g__;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243812_bin.37_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

characteristic: assembly software

characteristic: binning parameters

text: metawrap v1.3

characteristic: binning software

text: mouse digestive system

text: EBI-EMG

characteristic: broker name

text: CheckM

characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: uncultured Lachnospiraceae bacterium

characteristic: organism

text:
Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.

Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.

Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN.

text: uncultured Lachnospiraceae bacterium

text: Illumina NovaSeq 6000

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243812_bin.39_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

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text: mouse digestive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: feces

characteristic: environmental medium

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

characteristic: investigation type

text: mouse gut metagenome

text: digestive tube

characteristic: local environmental context

text: mouse gut metagenome

characteristic: metagenomic source

text: uncultured Lachnoclostridium sp.

characteristic: project name

characteristic: sample derived from

text: uncultured Lachnoclostridium sp.

text: Illumina NovaSeq 6000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Lachnoclostridium;s__

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR18243812_bin.5_metawrap_v1.3_MAG


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: EMG

characteristic: INSDC center name

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characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

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text: mouse digestive system

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characteristic: broker name

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characteristic: description

text: feces

text: Germany

characteristic: geographic location (country and/or sea)

text: metagenome-assembled genome

text: mouse gut metagenome

text: digestive tube

text: mouse gut metagenome

text: Bifidobacterium pseudolongum

characteristic: organism

text: Bifidobacterium pseudolongum

text: Illumina NovaSeq 6000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Bifidobacterium;s__Bifidobacterium pseudolongum

text: multi-marker approach

text: Metagenome-assembled genome: SRR18243812_bin.52_metawrap_v1.3_MAG

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: Haukeland University Hospital

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tag: Namespace:Haukeland University Hospital

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text: Blood culture

text: Norway

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text: Homo sapiens

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text: Streptococcus dysgalactiae

text: Streptococcus dysgalactiae

text: subspecies equisimilis


text: Portugal

characteristic: Country

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text: yes

characteristic: INSDC center name

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tag: Namespace:NATIONAL INSTITUTE OF HEALTH DR. RICARDO JORGE

characteristic: Submitter Id

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text: Listeria monocytogenes


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text: no

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text: raw milk cheese

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text: Listeria monocytogenes

text: Listeria monocytogenes


text: Portugal

text: no

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text: Dairy

text: Listeria innocua

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text: public

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text: ExPEC

characteristic: Pathotype

text: Susceptible

characteristic: ST

text: raw milk cheese

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text: Surveillance

text: Escherichia coli

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characteristic: serotype


characteristic: External Id

characteristic: INSDC center name

text: public

text: Portugal

text: ExPEC

text: MDR

text: raw milk cheese

text: Food

text: Dairy

text: Surveillance

text: Escherichia coli

text: Escherichia coli


text: University of Tartu, Estonia

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text: public

text: ena-upload-cli v0.6.1 @ Galaxy

tag: Namespace:University of Tartu, Estonia

text: University of Tartu KoroGenoEST3 consortium, Estonia

characteristic: collecting institution

text: Aare Abroi, Radko Avi, Taavi Päll, Heiki Niglas , Merit Pauskar, Arina Shablinskaja, Eveli Kallas, Andrio Lahesaare, Kai Truusalu, Dagmar Hoidmets, Olga Sadikova, Kaspar Ratnik, Hanna Sepp, Liidia Dotsenko, Jevgenia Epštein, Heleene Suija, Katrin Kaarna, Lili Milani, Mait Metspalu, Ott Eric Oopkaup, Ivar Koppel, Erik Jaaniso, Ivan Kuzmin, Uku Raudvere, Tuuli Reisberg, Hedi Peterson, Ulvi Gerst Talas, Paul Naaber, Mari-Anne Härma, Irja Lutsar, Kristi Huik, Diana Pilvar

characteristic: collector name

text: Nasopharyngeal/oropharyngeal swab from Estonian Covid-19 patient

text: Estonia

characteristic: geographic location (country and/or sea)

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characteristic: geographic location (longitude)

text: Northern Estonia

characteristic: geographic location (region and locality)

unit: year

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text: Severe acute respiratory syndrome coronavirus 2

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text: human

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text: University of Tartu, Estonia

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text: Estonia

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text: human

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text: active surveillance not initiated by an outbreak

text: PCR tiled amplicon WGS of SARS-Cov-2, monitoring random samples from active surveillance


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text: not provided

text: Homo sapiens

text: female

characteristic: organism

characteristic: title


text: University of Tartu, Estonia

characteristic: INSDC first public

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text: Estonia

characteristic: geographic location (longitude)

text: Southern Estonia

characteristic: geographic location (region and locality)

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text: Homo sapiens

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characteristic: ENA-CHECKLIST

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characteristic: External Id

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text: University of Tartu, Estonia

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text: human

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text: Homo sapiens

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characteristic: organism

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: ena-upload-cli v0.6.1 @ Galaxy

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characteristic: geographic location (region and locality)

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text: not provided

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text: Homo sapiens

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text: University of Tartu, Estonia

characteristic: INSDC first public

characteristic: INSDC last update

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characteristic: SRA accession

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characteristic: Submitter Id

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Tartu, Estonia

characteristic: INSDC center name

text: public

characteristic: INSDC status

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characteristic: description

text: Estonia

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Western Estonia

text: human

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text: University of Tartu, Estonia

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text: Homo sapiens

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text: Arrested Aneuploid cells; RPE1 hTERT wild type; Replicate 2

text: Homo sapiens

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text: Centro Ames

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tag: Namespace:Centro Ames

text: human

text: Representative NGS sequencing of metastatic colorectal cancer patients

text: Homo sapiens

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text: not provided

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text: hCov-19 Oxford Nanopore MinION reads obtained following the Artic v3 protocol

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text: Europe / Ireland / Clare

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text: Europe / Ireland / Cork

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text: Europe / Ireland / Dublin

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text: Europe / Ireland / Dublin

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characteristic: sample capture status

text: hCoV-19/Ireland/D-NVRL-S22IRL00471182/2022


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text: Irish Consortium for Sequencing Covid

text: National Virus Reference Laboratory

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text: Human coronavirus 2019

text: Ireland

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text: Europe / Ireland / Dublin

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text: Homo sapiens

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470462/2022

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text: hCov-19 Oxford Nanopore MinION reads obtained following the Artic v3 protocol

characteristic: description

text: Ireland

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Europe / Ireland / Dublin

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470510/2022

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470445/2022


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text: hCoV-19/Ireland/D-NVRL-S22IRL00470521/2022

characteristic: virus identifier


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text: hCoV-19/Ireland/D-NVRL-S22IRL00470489/2022

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text: Europe / Ireland / Dublin

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text: Homo sapiens

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text: Severe acute respiratory syndrome coronavirus 2

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470513/2022


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text: Europe / Ireland / Dublin

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text: active surveillance in response to outbreak

text: hCoV-19/Ireland/D-NVRL-S22IRL00470465/2022

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470484/2022

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470488/2022


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text: Europe / Ireland / Dublin

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text: Homo sapiens

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470397/2022

characteristic: virus identifier


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text: hCoV-19/Ireland/D-NVRL-S22IRL00470464/2022

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characteristic: geographic location (latitude)

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text: Europe / Ireland / Dublin

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text: Homo sapiens

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characteristic: sample capture status

text: hCoV-19/Ireland/D-NVRL-S22IRL00470446/2022


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text: hCoV-19/Ireland/D-NVRL-S22IRL00470398/2022

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text: hCoV-19/Ireland/D-NVRL-S22IRL00470537/2022