characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: assembly quality

text: metaSPAdes v3.15.3

text: metaWRAP v1.3

text: Human reproductive system

text: EBI-EMG

text: CheckM

text: This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR17635820 of study SRP355266.

characteristic: description

text: Vagina

characteristic: environmental medium

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome-assembled genome

text: vaginal metagenome

text: Vagina

characteristic: local environmental context

text: vaginal metagenome

text: uncultured Megasphaera sp.

characteristic: organism

text: The overarching goal of the VMRC is to identify correlates of stability and resilience in the vaginal microbiota and leverage these features to devise a framework for an intervention to maintain/restore vaginal health.

text: uncultured Megasphaera sp.

text: Illumina HiSeq 4000

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Megasphaera;s__

text: multi-marker approach

text: Metagenome-assembled genome: SRR17635820_bin.3_metaWRAP_v1.3_MAG

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: EMG

text: public

text: Many fragments with little to no review of assembly other than reporting of standard assembly statistics

text: metaSPAdes v3.15.3

characteristic: assembly software

text: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.

characteristic: binning parameters

text: metaWRAP v1.3

characteristic: binning software

text: Human reproductive system

characteristic: broad-scale environmental context

text: EBI-EMG

characteristic: broker name

characteristic: collection date

characteristic: completeness score

text: CheckM

characteristic: completeness software

characteristic: contamination score

text: Vagina

text: USA

unit: DD

text: metagenome-assembled genome

characteristic: investigation type

text: vaginal metagenome

text: Vagina

text: vaginal metagenome

characteristic: metagenomic source

text: Gardnerella vaginalis

characteristic: project name

characteristic: sample derived from

text: Gardnerella vaginalis

text: Illumina HiSeq 4000

characteristic: sequencing method

text: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Gardnerella;s__Gardnerella vaginalis

characteristic: taxonomic classification

text: multi-marker approach

characteristic: taxonomic identity marker

text: Metagenome-assembled genome: SRR17635820_bin.4_metaWRAP_v1.3_MAG


E-MTAB-12353:Sample 45

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Quadram Institute Bioscience

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12353:Sample 45

tag: Namespace:Quadram Institute Bioscience

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: The transposon mutant library in E. coli used in this study was described by Yasir et al. (2020) and contains over 800,000 unique mutants, which roughly equates to an insertion every 6 bp. The transposon mutant library in S. Typhimurium described by Holden et al. 2022 (in press) is also very dense, containing approximately 500,000 unique mutants and an insertion every 10 bp. Approximately 10^7 CFU/mL of transposon mutant library was added to 5 mL lysogeny broth (LB) in an untreated 6-well plate (Sigma). The minimum inhibitory concentration (MIC) of acriflavine in the parent E. coli and S. Typhimurium strains was determined to be 256 mg/L following the microbroth dilution method (EUCAST, 2021). Conditions were either left untreated or were supplemented with subinhibitory (64 mg/L) or inhibitory (256 mg/L) concentrations of acriflavine. Each condition was also prepared with or without 125 mg/L PAβN to elucidate genes involved in efflux specifically. Each condition was also prepared with and without 1 mM IPTG to induce expression from the transposon-located tac promoter, crucial for investigating how expression affect survival and for assaying essential genes. Two independent replicates were prepared for each condition. Plates were incubated at 30 ⁰C with light agitation at 60 rpm. Following 24 hours incubation, the cultures were centrifuged at 2100 x g for 10 minutes to form pellets, from which genomic DNA was extracted following the protocol described by Trampari et al. (2021) Genomic DNA was extracted following the protocol described by Trampari et al. (2021, NPJ Biofilms Microbiomes) Sequencing libraries were prepared following the protocol outlined by ( Yasir et al. (2020, Genom Res). Genomic DNA was fragmented using the MuSeek DNA library preparation kit (Invitrogen) and purified with 1.5x volume of AMPure beads (Beckman Coulter). Fragments containing the transposon were amplified by PCR using customised primers specific for the transposon and for the MuSeek tags. The PCR products were clean twice with 0.6x volume and 1x volume of AMPure beads to purify and size-select fragments between 300-500 bp.

characteristic: description

text: Escherichia coli str. K-12 substr. MG1655

characteristic: organism

characteristic: replicate

text: Sample 45

characteristic: title


E-MTAB-12353:Sample 46

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 46

text: ArrayExpress

characteristic: broker name

text: Sample 46


E-MTAB-12353:Sample 41

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 41

text: ArrayExpress

text: Sample 41


E-MTAB-12353:Sample 42

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 42

text: ArrayExpress

text: Sample 42


E-MTAB-12353:Sample 37

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 37

text: ArrayExpress

text: Sample 37


E-MTAB-12353:Sample 38

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 38

text: ArrayExpress

text: Sample 38


E-MTAB-12353:Sample 33

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 33

text: ArrayExpress

text: Sample 33


E-MTAB-12353:Sample 34

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 34

text: ArrayExpress

text: Sample 34


E-MTAB-12353:Sample 29

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 29

text: ArrayExpress

text: Sample 29


E-MTAB-12353:Sample 30

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 30

text: ArrayExpress

text: Sample 30


E-MTAB-12353:Sample 25

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 25

text: ArrayExpress

text: Sample 25


E-MTAB-12353:Sample 26

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 26

text: ArrayExpress

text: Sample 26


E-MTAB-12353:Sample 47

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 47

text: ArrayExpress

text: Sample 47


E-MTAB-12353:Sample 48

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 48

text: ArrayExpress

text: Sample 48


E-MTAB-12353:Sample 43

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 43

text: ArrayExpress

text: Sample 43


E-MTAB-12353:Sample 44

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 44

text: ArrayExpress

text: Sample 44


E-MTAB-12353:Sample 39

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 39

text: ArrayExpress

text: Sample 39


E-MTAB-12353:Sample 40

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 40

text: ArrayExpress

text: Sample 40


E-MTAB-12353:Sample 35

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 35

text: ArrayExpress

text: Sample 35


E-MTAB-12353:Sample 36

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 36

text: ArrayExpress

text: Sample 36


E-MTAB-12353:Sample 31

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 31

text: ArrayExpress

text: Sample 31


E-MTAB-12353:Sample 32

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 32

text: ArrayExpress

text: Sample 32


E-MTAB-12353:Sample 27

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 27

text: ArrayExpress

text: Sample 27


E-MTAB-12353:Sample 28

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 28

text: ArrayExpress

text: Sample 28


E-MTAB-12353:Sample 21

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 21

text: ArrayExpress

text: Salmonella enterica subsp. enterica serovar Typhimurium

text: Sample 21


E-MTAB-12353:Sample 22

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 22

text: ArrayExpress

text: Sample 22


E-MTAB-12353:Sample 17

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 17

text: ArrayExpress

text: Sample 17


E-MTAB-12353:Sample 18

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 18

text: ArrayExpress

text: Sample 18


E-MTAB-12353:Sample 13

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 13

text: ArrayExpress

text: Sample 13


E-MTAB-12353:Sample 14

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 14

text: ArrayExpress

text: Sample 14


E-MTAB-12353:Sample 10

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 10

text: ArrayExpress

text: Sample 10


E-MTAB-12353:Sample 9

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 9

text: ArrayExpress

text: Sample 9


E-MTAB-12353:Sample 5

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 5

text: ArrayExpress

text: Sample 5


E-MTAB-12353:Sample 6

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 6

text: ArrayExpress

text: Sample 6


E-MTAB-12353:Sample 1

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 1

text: ArrayExpress

text: Sample 1


E-MTAB-12353:Sample 2

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 2

text: ArrayExpress

text: Sample 2


E-MTAB-12353:Sample 23

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 23

text: ArrayExpress

text: Sample 23


E-MTAB-12353:Sample 24

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 24

text: ArrayExpress

text: Sample 24


E-MTAB-12353:Sample 19

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 19

text: ArrayExpress

text: Sample 19


E-MTAB-12353:Sample 20

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 20

text: ArrayExpress

text: Sample 20


E-MTAB-12353:Sample 15

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 15

text: ArrayExpress

text: Sample 15


E-MTAB-12353:Sample 16

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 16

text: ArrayExpress

text: Sample 16


E-MTAB-12353:Sample 11

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 11

text: ArrayExpress

text: Sample 11


E-MTAB-12353:Sample 12

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 12

text: ArrayExpress

text: Sample 12


E-MTAB-12353:Sample 7

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 7

text: ArrayExpress

text: Sample 7


E-MTAB-12353:Sample 8

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 8

text: ArrayExpress

text: Sample 8


E-MTAB-12353:Sample 3

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 3

text: ArrayExpress

text: Sample 3


E-MTAB-12353:Sample 4

text: Quadram Institute Bioscience

text: public

text: E-MTAB-12353:Sample 4

text: ArrayExpress

text: Sample 4


text: BIMSB

text: public

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: RNA-seq samples were prepared in three biological replicates. Cells were harvested and counted, 20% Drosophila S2 cells were added as a spike-in control. Cell pellets were resuspended in 1 mL TRI reagent (Sigma). 200 µL Chloroform (Applichem) were added, samples were mixed and centrifuged at 4°C, max. speed for 15 min. The upper phase was transferred to a new tube and subjected to isopropanol precipitation. 20 µg of RNA were treated with 40 U DNase I (Roche) at 37°C for 30 min and purified by phenol-chloroform extraction and Ethanol precipitation. rRNA was depleted. DIDO and SHARP RNA-seq libraries were prepared with NEBNext rRNA depletion kit v2 (Human/Mouse/Rat) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions. RBM15 RNA-seq libraries were prepared using Lexogen RiboCop rRNA Depletion kit HMR V2 and Lexogen CORALL Total RNA-seq Library Prep kit according to the manufacturer’s instructions. 600 ng total RNA were used as an input for rRNA depletion.

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: DIDO knockout

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BIMSB

text: public

text: ArrayExpress

text: human

text: embryo stage

text: normal

text: DIDO wild type

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: BIMSB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: embryo stage

text: normal

text: DIDO SPOC domain deletion

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: BIMSB

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: human

characteristic: description

text: embryo stage

text: normal

text: RBM15 knockout

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

text: Homo sapiens


text: BIMSB

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: embryo stage

text: normal

text: RBM15 wild type

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

characteristic: title


text: BIMSB

text: public

text: ArrayExpress

text: human

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: RBM15 SPOC domain deletion

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BIMSB

text: public

text: ArrayExpress

text: human

text: embryo stage

text: normal

text: SHARP wild type

text: Homo sapiens

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BIMSB

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: embryo stage

text: normal

text: SHARP SPOC domain deletion

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: BIMSB

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: B cell

text: house mouse

text: Protocols: Splenic B cells were isolated from C57BL/6 mice by CD43 depletion with magnetic anti-mouse CD43 microbeads (Miltenyi Biotech, Cat#130-049-801) according to the manufacturer’s instructions. To induce B cell activation and terminal differentiation, purified B cells (density: 1x106 cells/mL; 4x106 cells per well) were cultured in the presence of recombinant mouse IL-4 (25ng/mL; R&D; Cat#404-ML) and LPS (20µg/mL; Sigma; Cat#L2880) over night. 24h after isolation, B cells were collected (300xg, 5min, 4°C) and resuspended in B cell medium supplemented with 8µg/mL polybrene (EMD Millipore, #TR-1003) at a density of 2x106 cells/mL. To introduce IRF4 wildtype and mutant, 4x106 B cells per well were plated in 2mL on 6-well plates that had been coated with RetroNectin (Takara, #T100B; 25µg/mL; 4°C, overnight), blocked with 2% BSA (in PBS, 1h, RT) and pre-loaded with the respective retroviral particles (1h, 37°C). Retroviral transduction was performed by the addition of 2mL of the respective retroviral supernatant and subsequent centrifugation (800xg, 90min, 32°C). 24h after transduction, B cells were collected (300xg, 5min, 4°C), resupended in new B cell medium and cultured (density: 1x106 cells/mL; 4x106 cells per well) for another 72h (FACS for RNA-seq, flow cytometric analysis of plasma cell differentiation) in the presence of recombinant mouse IL-4 (25ng/mL; R&D; Cat#404-ML) and LPS (20µg/mL; Sigma; Cat#L2880). Nucleic acids were extracted as described in the accompanying manuscript Nucleic acids were extracted with RNeasy Micro Kit (Qiagen) per provider's protocol

text: adult

text: normal

text: MIG empty vector

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

characteristic: strain


text: BIMSB

text: public

characteristic: SRA accession

text: E-MTAB-12352:mm_spleen_T95R_br1.fastq.gz

characteristic: Submitter Id

text: ArrayExpress

text: B cell

text: house mouse

characteristic: description

text: adult

text: normal

text: IRF4 T95R mutation

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus


text: BIMSB

text: public

text: E-MTAB-12352:mm_spleen_T95R_br2.fastq.gz

text: ArrayExpress

characteristic: broker name

text: B cell

text: house mouse

characteristic: common name

text: adult

characteristic: developmental stage

text: normal

text: IRF4 T95R mutation

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BIMSB

text: public

text: E-MTAB-12352:mm_spleen_T95R_br3.fastq.gz

text: ArrayExpress

text: B cell

text: house mouse

text: adult

text: normal

characteristic: disease

text: IRF4 T95R mutation

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: BIMSB

characteristic: INSDC center name

text: public

text: ArrayExpress

text: B cell

text: house mouse

text: adult

text: normal

text: wild type genotype

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus


text: UNIVERSITY OF OXFORD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:UNIVERSITY OF OXFORD

text: University of Oxford

characteristic: collecting institution

characteristic: collection date

text: Modernising Medical Microbiology

characteristic: collector name

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

text: not collected

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

text: not collected

characteristic: host sex

text: not collected

characteristic: host subject id

text: RT-PCR

characteristic: influenza test method

text: P

characteristic: influenza test result

text: Influenza A virus

text: Influenza A virus

text: Influenza genome sequencing


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIVERSITY OF OXFORD

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: University of Oxford

text: Modernising Medical Microbiology

text: United Kingdom

text: human

text: not collected

text: Homo sapien

text: not collected

text: not collected

text: RT-PCR

text: N

text: Influenza A virus

characteristic: organism

text: Influenza A virus

text: Influenza genome sequencing

characteristic: title


E-MTAB-12238:Sample 1

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 1

tag: Namespace:Department of Molecular Cell Biology, Weizmann Institute of Science

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: DNA Purification from BM

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 1


E-MTAB-12238:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 10

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD45 positive

characteristic: time

text: Sample 10

characteristic: title

text: human CD45 positive out of mouse bone marrow 80

characteristic: xenograft


E-MTAB-12238:Sample 100

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 100

text: Repli G

text: ArrayExpress

characteristic: broker name

text: hematopoietic multipotent progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 100


E-MTAB-12238:Sample 101

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 101

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

characteristic: subclone

characteristic: time

text: Sample 101

text: human CD45 positive out of mouse bone marrow 74


E-MTAB-12238:Sample 102

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 102

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: Sample 102

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 103

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 103

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 103


E-MTAB-12238:Sample 104

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 104

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 104


E-MTAB-12238:Sample 105

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 105

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 105

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 74

characteristic: xenograft


E-MTAB-12238:Sample 106

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 106

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 106


E-MTAB-12238:Sample 107

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 107

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 107


E-MTAB-12238:Sample 108

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 108

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 108

characteristic: title


E-MTAB-12238:Sample 109

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 109

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 109


E-MTAB-12238:Sample 11

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 11

text: Repli G

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 11


E-MTAB-12238:Sample 110

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 110

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 110

characteristic: title


E-MTAB-12238:Sample 111

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 111

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 111

text: human CD45 positive out of mouse bone marrow 75

characteristic: xenograft


E-MTAB-12238:Sample 112

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 112

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: subclone

characteristic: time

text: Sample 112


E-MTAB-12238:Sample 113

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 113

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Miltenyi column -CD34+ Enrichment by magnetic beads

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive ; CD34 positive

text: Sample 113

characteristic: title


E-MTAB-12238:Sample 114

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 114

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 114


E-MTAB-12238:Sample 115

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 115

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 115


E-MTAB-12238:Sample 116

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 116

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 116

characteristic: title


E-MTAB-12238:Sample 117

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 117

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 117


E-MTAB-12238:Sample 118

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 118

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 118


E-MTAB-12238:Sample 119

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 119

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 119

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 75

characteristic: xenograft


E-MTAB-12238:Sample 12

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 12

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 12


E-MTAB-12238:Sample 120

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 120

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 120


E-MTAB-12238:Sample 121

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 121

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 121

characteristic: title


E-MTAB-12238:Sample 122

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 122

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 122


E-MTAB-12238:Sample 123

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 123

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 123


E-MTAB-12238:Sample 124

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 124

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 124

characteristic: title


E-MTAB-12238:Sample 125

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 125

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 125

text: human CD45 positive out of mouse bone marrow 76

characteristic: xenograft


E-MTAB-12238:Sample 126

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 126

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: subclone

characteristic: time

text: Sample 126


E-MTAB-12238:Sample 127

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 127

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 127

characteristic: title


E-MTAB-12238:Sample 128

characteristic: INSDC last update

text: public

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text: E-MTAB-12238:Sample 128

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 128


E-MTAB-12238:Sample 129

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 129

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 129


E-MTAB-12238:Sample 13

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 13

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 13

characteristic: title


E-MTAB-12238:Sample 130

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 130

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 130


E-MTAB-12238:Sample 131

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 131

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 positive

characteristic: subclone

characteristic: time

text: Sample 131

text: 1:10 human CD45 positive out of mouse bone marrow 76

characteristic: xenograft


E-MTAB-12238:Sample 132

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 132

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: Sample 132

characteristic: title


E-MTAB-12238:Sample 133

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 133

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 133


E-MTAB-12238:Sample 134

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 134

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 134


E-MTAB-12238:Sample 135

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 135

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: Homo sapiens

text: male

text: Sample 135

characteristic: title


E-MTAB-12238:Sample 136

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 136

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 136


E-MTAB-12238:Sample 137

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 137

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 137


E-MTAB-12238:Sample 138

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 138

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 138

characteristic: title


E-MTAB-12238:Sample 139

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 139

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 139

text: human CD45 positive out of mouse bone marrow 1

characteristic: xenograft


E-MTAB-12238:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 14

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

characteristic: time

text: Sample 14


E-MTAB-12238:Sample 140

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 140

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 140

characteristic: title


E-MTAB-12238:Sample 141

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 141

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 141

text: human CD45 positive out of mouse bone marrow 88

characteristic: xenograft


E-MTAB-12238:Sample 142

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 142

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 142


E-MTAB-12238:Sample 143

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 143

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 143

characteristic: title


E-MTAB-12238:Sample 144

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 144

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 144


E-MTAB-12238:Sample 145

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 145

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 145


E-MTAB-12238:Sample 146

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 146

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 146

characteristic: title


E-MTAB-12238:Sample 147

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 147

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: cell count

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 147

text: 1:10 human CD45 positive out of mouse bone marrow 1

characteristic: xenograft


E-MTAB-12238:Sample 148

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 148

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 148


E-MTAB-12238:Sample 149

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 149

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive

text: Sample 149

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 88


E-MTAB-12238:Sample 15

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 15

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 15

text: 1:10 human CD45 positive out of mouse bone marrow 11

characteristic: xenograft


E-MTAB-12238:Sample 150

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 150

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD34 positive

characteristic: time

text: Sample 150


E-MTAB-12238:Sample 151

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 151

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 151

characteristic: title


E-MTAB-12238:Sample 152

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 152

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 152


E-MTAB-12238:Sample 153

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 153

text: ArrayExpress

characteristic: broker name

text: hematopoietic multipotent progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 153


E-MTAB-12238:Sample 154

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 154

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 154

characteristic: title


E-MTAB-12238:Sample 155

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 155

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 155

text: human CD45 positive out of mouse bone marrow 4

characteristic: xenograft


E-MTAB-12238:Sample 156

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 156

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 156


E-MTAB-12238:Sample 157

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 157

text: Repli G

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 157

characteristic: title


E-MTAB-12238:Sample 158

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 158

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 158


E-MTAB-12238:Sample 159

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 159

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 159

text: human CD45 positive out of mouse bone marrow 89

characteristic: xenograft


E-MTAB-12238:Sample 16

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 16

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: cell count

text: relapse

text: acute myeloid leukemia B phenotype

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: Sample 16

characteristic: title


E-MTAB-12238:Sample 160

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 160

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 160


E-MTAB-12238:Sample 161

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 161

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 161


E-MTAB-12238:Sample 162

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 162

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 162

characteristic: title


E-MTAB-12238:Sample 163

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 163

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 163


E-MTAB-12238:Sample 164

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 164

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 164


E-MTAB-12238:Sample 165

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 165

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: cell count

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 165

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 4

characteristic: xenograft


E-MTAB-12238:Sample 166

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 166

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 166


E-MTAB-12238:Sample 167

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 167

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive

characteristic: time

text: Sample 167

text: 1:10 human CD45 positive out of mouse bone marrow 89


E-MTAB-12238:Sample 168

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 168

text: Repli G

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive

text: Sample 168

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 169

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 169

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 169


E-MTAB-12238:Sample 17

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 17

text: Repli G

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 17

text: 1:10 human CD45 positive out of mouse bone marrow 80


E-MTAB-12238:Sample 170

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: SRA accession

text: E-MTAB-12238:Sample 170

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 170

characteristic: title


E-MTAB-12238:Sample 171

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12238:Sample 171

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 171