characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-mutation-reporter_shScramble-Ago2-Hbs1l_Neurite_2

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: neuron projection

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-mutation-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-mutation-reporter_shScramble-Ago2-Hbs1l_Neurite_3

tag: Namespace:Berlin Institute for Medical Systems Biology (BIMSB) Max Delbruck Center for Molecular Medicine

unit: day

text: ArrayExpress

text: neuron of cerebral cortex

text: neuron projection

characteristic: cellular component

text: house mouse

characteristic: common name

text: Protocols: Soma samples were collected from top of the filter membrane and neurite samples form the cleaned membranes at DIV9. See also: “Ludwik et al. 2019: Genome-wide analysis of RNA and protein localization and local translation in mESC-derived neurons” Cortical neurons were isolated from E14 and P0 pups and plated on microporous membranes in 6-well plates. Astrocytes were pre-seeded on the 6-well dishes around one week in advance of plating. Cells were cultured as previously described (Kaech & Banker, 2006). Neurons were transfected using lentiviral particles carrying either shRNA expression constructs or GFP constructs with inserts from one of the reporter library pools or. Viral particles were applied onto the neurons between DIV1 and DIV5. After collection samples were immediately transferred into Trizol and RNA extraction was performed according to manufacturer’s instructions (see also Ludwik et al. 2019). 100-200ng of total RNA was reverse transcribed into cDNA with a primer complementary to the 3' adapter flanking the tiles. UMIs were introduced during the second strand synthesis with a pool of primers complementary to the 5' adapter flanking the tiles.

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-mutation-reporter

characteristic: genetic modification

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

text: libraries contain only the Nzip-reporter inserts

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: Berlin Institute for Medical Systems Biology (BIMSB) Max Delbruck Center for Molecular Medicine

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-mutation-reporter_shScramble-Ago2-Hbs1l_Soma_1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-mutation-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-mutation-reporter_shScramble-Ago2-Hbs1l_Soma_2

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

characteristic: cellular component

text: house mouse

characteristic: common name

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-mutation-reporter

characteristic: genetic modification

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: mixed


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10902:PCN_Nzip-mutation-reporter_shScramble-Ago2-Hbs1l_Soma_3

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-mutation-reporter

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Neurite_1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: neuron projection

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Neurite_2

text: ArrayExpress

text: neuron of cerebral cortex

text: neuron projection

characteristic: cellular component

text: house mouse

characteristic: common name

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-reporter

characteristic: genetic modification

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: mixed


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Neurite_3

text: ArrayExpress

text: neuron of cerebral cortex

text: neuron projection

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Soma_1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Soma_2

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

characteristic: cellular component

text: house mouse

characteristic: common name

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-reporter

characteristic: genetic modification

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: mixed


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10902:PCN_Nzip-reporter_Depolarised_Soma_3

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Neurite_1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: neuron projection

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Neurite_2

text: ArrayExpress

text: neuron of cerebral cortex

text: neuron projection

characteristic: cellular component

text: house mouse

characteristic: common name

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-reporter

characteristic: genetic modification

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: mixed


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Neurite_3

text: ArrayExpress

text: neuron of cerebral cortex

text: neuron projection

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Soma_1

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

characteristic: broker name

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

characteristic: organism

text: Mus musculus

text: brain

text: Mus musculus

text: mixed

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Soma_2

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

characteristic: cellular component

text: house mouse

characteristic: common name

characteristic: description

text: neonate

characteristic: developmental stage

text: normal

characteristic: disease

text: GFP-Nzip-reporter

characteristic: genetic modification

text: wild type genotype

text: 4 to 8 individuals from litter11

text: Mus musculus

text: Mus musculus

text: brain

text: Mus musculus

text: mixed


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

text: E-MTAB-10902:PCN_Nzip-reporter_DepolarisedControl_Soma_3

text: ArrayExpress

text: neuron of cerebral cortex

text: perikaryon

text: house mouse

text: Protocols: Soma samples were collected from top of the filter membrane and neurite samples form the cleaned membranes at DIV9. See also: “Ludwik et al. 2019: Genome-wide analysis of RNA and protein localization and local translation in mESC-derived neurons” Cortical neurons were isolated from E14 and P0 pups and plated on microporous membranes in 6-well plates. Astrocytes were pre-seeded on the 6-well dishes around one week in advance of plating. Cells were cultured as previously described (Kaech & Banker, 2006). Neurons were transfected using lentiviral particles carrying either shRNA expression constructs or GFP constructs with inserts from one of the reporter library pools or. Viral particles were applied onto the neurons between DIV1 and DIV5. After collection samples were immediately transferred into Trizol and RNA extraction was performed according to manufacturer’s instructions (see also Ludwik et al. 2019). 100-200ng of total RNA was reverse transcribed into cDNA with a primer complementary to the 3' adapter flanking the tiles. UMIs were introduced during the second strand synthesis with a pool of primers complementary to the 5' adapter flanking the tiles.

text: neonate

text: normal

text: GFP-Nzip-reporter

text: wild type genotype

characteristic: genotype

text: 4 to 8 individuals from litter11

characteristic: individual

text: Mus musculus

text: Mus musculus

text: brain

characteristic: organism part

characteristic: protocol

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain


characteristic: ENA-LAST-UPDATE

text: UNIVERSITY OF OXFORD

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF OXFORD

characteristic: Submitter Id

text: University of Oxford

characteristic: collecting institution

characteristic: collection date

text: Modernising Medical Microbiology

characteristic: collector name

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

text: not collected

characteristic: host health state

text: Homo sapien

characteristic: host scientific name

text: not collected

characteristic: host sex

text: not collected

characteristic: host subject id

text: RT-PCR

characteristic: influenza test method

text: P

characteristic: influenza test result

text: Influenza A virus

characteristic: organism

text: Influenza A virus

text: Influenza genome sequencing

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Tenth People's Hospital. Tongji University

text: ArrayExpress

characteristic: broker name

text: aureobasidin A

characteristic: compound

text: Protocols: Strains were maintained at -80˚C. Strains were streaked on YPD plates. The plates were incubated at 37˚C overnight. Then several colonies were picked up and suspended in DDW. Cell density was adjusted to 10,000 cells/ml, then 100µl of cell suspension was plated on YPD plates. The plates were incubated at 37˚C overnight. Colonies were picked up and suspended in DDW, then cell pellets were obtained by centrifuging at highest speed for 1min. We used the phenol-chloroform method to extract Genomic DNA. Cell pellets were suspended in 200µl of lysis buffer, add 100µl of acid washed glass beads, and 0,2ml phenol:chloroform:isoamyl alcohol (25:24:1). Vortex for 30min at 4˚C. Add 200µl TE pH7.5. Centrifuge for 5min at maximum speed. Transfer aqueous layer to a new 1.5ml EP tube. Add 1ml 100% ethanol. Precipitate 1h at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard the supernatant. Add 400µl TE pH 7.5. Add 3ul of RNase A (10mg/ml). Incubate at 37˚C for 30min. Add 10µl of 3M NaOAc and 1ml of 100% ethanol. Mix by inversion. Precipitate 30min at -20˚C. Pellet DNA by centrifuging for 10min at maximum speed. Discard supernatant. Air-dry the pellet overnight. Resuspend in 50µl water. 1µg Genomic DNA was randomly fragmented by Covaris, followed by fragments selection by Agencourt AMPure XP-Medium kit to an average size of 200~400 bp. Selected fragments were end repaired and 3\adenylate, then the adaptors were ligated to the end of these 3\adenylate fragments. The products were amplified by PCR and purified by the Agencourt AMPure XP-Medium kit. The purified double stranded PCR products were heat denatured to single strand, then circularised by the splint oligo sequence. The single strand circle DNA (ssCirDNA) were formatted as the final library and qualified by QC.

characteristic: description

unit: nanogram per milligram

characteristic: dose

characteristic: genotype

text: YPD broth

characteristic: growth condition

text: Candida albicans

text: Candida albicans

text: whole organism

characteristic: organism part

text: aureobasidin A tolerant

characteristic: phenotype

text: Candida albicans

characteristic: strain


text: Tenth People's Hospital. Tongji University

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: aureobasidin A

text: YPD broth

text: Candida albicans

characteristic: organism

text: Candida albicans

text: whole organism

text: aureobasidin A tolerant

text: Candida albicans

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: KAROLINSKA INSTITUTE

characteristic: INSDC center name

text: public

tag: Namespace:KAROLINSKA INSTITUTE

text: ArrayExpress

text: human

characteristic: common name

text: Protocols: Medium was discarded and plates were shortly submerged in liquid nitrogen to snap freeze cells. 2x lysis buffer (100 mM Tris pH 7.5, 200 mM NaCl, 40 mM MgCl2, 2 mM DTT, 200 µg/ml chloramphenicol, 200 µg/ml cycloheximide, 2 % Triton X-100, 2x Complete EDTA-free 725 protease inhibitor cocktail (Roche), 4000 U/ml TURBO DNase I (Thermo Fisher)) was added dropwise on the plates and lysates were collected using cell scrapers. Lysis was completed by triturating samples 10 x with a 20-G needle and cell debris were removed by centrifugation (13,000 xg, 20 min, 4 °C). 200 µl of supernatant were subjected to RNase treatment for 30 min at RT (450 U, Ambion RNase I, Thermo Fisher). RNase treatment was stopped by the addition of 10 µl RNase inhibitor (1 U/µl, SUPERase-In, Thermo Fisher), which was followed by a short centrifugation step to remove insoluble material (5,000 xg, 5 min). Supernatants were loaded on 10-30 % sucrose gradients (50 mM Tris pH 7.4, 100 mM NaCl, 20 mM MgCl2, 1 mM DTT, 100 µg/ml chloramphenicol, 100 µg/ml cycloheximide, 1x Complete EDTA-free protease inhibitor cocktail (Roche), 40 U/ml RNase inhibitor (SUPERase-In, Thermo Fisher) in 11 x 34 mm tubes (Beckman Coulter) and run at 39.000 rpm for 2 h 15 min in a TLS-55 rotor (Beckman Coulter). Afterwards, 100 µl fractions were collected, and 10 µl of each were used for western blot analysis. The remaining volumes of fractions 11-16, representing monosome containing fractions, were combined, and further processed for mitoribosome profiling. HEK WT and mtRF1 KO cells were grown to 80 % confluency on 15-cm dishes. RNA was isolated from cells using TRIZOL reagent (Thermo Fisher) according to manufacturer’s instructions with the following minor changes: RNA solution was incubated with isopropanol for 30 min, and the pellet was washed with absolute ethanol. RNA was solubilized at 4 °C in water containing 40 units of RNase block (Agilent Technologies). Isolated RNA was heated at 80 °C for 3 min, put on ice for 1 min, mixed with Gel Loading Buffer II (Thermo Fisher) and loaded onto a 15 % Novex TBE-Urea gel (Thermo Fisher). The gel was run in 1x TBE buffer at 100 V for ~2 h. After completion of the run the gel was stained with 1x SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher) in 1x TBE. Nucleic acids were visualized and bands referring from 30 to 40 nt were excised. RNA was extracted from gel slices in 600 µl RNA extraction buffer (300 mM NaOAc pH 5.5, 1 mM EDTA, 0.25 % SDS) rotating at 4 °C ON. The next day, RNA was precipitated by adding 1.8 ml ice-cold EtOH together with 4 µl GlycoBlue Coprecipitant (Thermo Fisher) and subsequent storage at -80 °C ON. Precipitated RNA was pelleted by centrifugation (5,000 xg, 10 min, 4 °C). Pellet was once washed with 1 ml EtOH, dried for ~5 min and resuspended in 15 µl 10 mM Tris pH 7.5 supplemented with 1 µl RNase inhibitor (SUPERase-In, Thermo Fisher). Samples were heated at 80 °C for 2 min before placing on ice. Next, 3’ phosphates were removed by T4 PNK treatment (1 µl T4 PNK (NEB) added) in 1x T4 PNK buffer (NEB) at 37 °C for 2 h. Reaction was stopped by heat inactivation (65 °C, 10 min). RNA was pelleted by addition of 70 µl water, 2 µl GlycoBlue Coprecipitant (Thermo Fisher), 10 µl 1 M NaOAc and 300 µl EtOH and subsequent storage at -80 °C. RNA was washed and dried as described earlier and finally resuspended in 7 µl 10 mM Tris pH 7.5 supplemented with 1 µl RNase inhibitor. RNA libraries were generated using TrueSeq Small RNA Library Prep Kit (Illumina) according to manufacturer’s protocol with some modifications. Preparation was started by adding 1.2 µl adenylated RA3 to dephosphorylated RNA and incubating the mixture at 80 °C for 2 min. Afterwards, ligation was performed by addition of 2 µl of T4 RNA Ligase 2 (truncated K227Q), 2 µl T4 RNA Ligase 2 buffer and 6 µl PEG8000 (all components from NEB) and incubation at 14 °C ON. RNA was precipitated as described earlier, 20 µl 3 M NaOAc and 600 µl EtOH) and resuspended in 4 µl 10 mM Tris pH 7.5. Ligation products were then purified on a 15 % Novex TBE-Urea gel (Thermo Fisher), extracted, and precipitated as described earlier. Next, RNA was resuspended in 13 µl 10 mM Tris pH 7.5 supplemented with 1 µl RNase inhibitor. Then, 2 mM ATP, 2 µl 10x T4 PNK buffer and 2 µl T4 PNK (NEB) were added, and the reaction mixture was incubated for 2 h at 37 °C, followed by heat inactivation (65 °C, 10 min). RNA was precipitated and resuspended in 13 µl 10 mM Tris pH 7.5 supplemented with 1 µl RNase inhibitor. Thereafter, RNA footprints were ligated with 5’ RNA adaptor (RA5, Illumina) by adding 1.2 µl RA5, 2 µl 10x T4 buffer and 2 µl T4 RNA ligase 38 (Promega) and incubating at 14 °C ON. RNA was precipitated and resuspended in 3 µl 10 mM Tris pH 7.5. Reverse transcription and PCR amplification of library Reverse transcription was performed using RNA RT primers from TrueSeq Small RNA Library Prep Kit (Illumina) and SuperScript III First-Strand Synthesis System (Thermo Fisher) according to manufacturer’s protocol. Afterwards, 2 µl of RT products were PCR amplified using Phusion High-Fidelity PCR master mix (NEB) and DNA primers from TrueSeq Small RNA Library Prep Kit (Illumina). The PCR products were purified using QIAquick PCR Purification Kit (Qiagen), precipitated as usual and resolved on a 10 % Novex non-denaturing TBE gel (Thermo Fisher) using 1x TBE running buffer. PCR products were excised and extracted using DNA extraction buffer (300 mM NaCl, 10 mM Tris pH 8, 1 mM EDTA). Subsequently, PCR products were precipitated and pelleted.

text: embryo stage

characteristic: developmental stage

text: normal

characteristic: disease

text: wild type genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: KAROLINSKA INSTITUTE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: human

characteristic: description

text: embryo stage

text: normal

text: RF1 -/-

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens


text: Institut of Tropical Medicine

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Institut of Tropical Medicine

text: Institute of Tropical Medicine, Tuebingen, Germany

characteristic: collecting institution

characteristic: collection date

text: Jana Held

characteristic: collector name

text: Microfilaria

text: qPCR

characteristic: diagnostic method

text: Gabon

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

unit: year

characteristic: host age

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

text: female

characteristic: host sex

characteristic: isolate

text: blood

characteristic: isolation source host-associated

text: Mansonella perstans

text: Mansonella perstans


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institut of Tropical Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Jana Held

text: Microfilaria

text: qPCR

text: Gabon

text: human

text: Homo sapiens

text: male

text: blood

text: Mansonella sp. 'DEUX'

characteristic: organism

text: Mansonella sp. 'DEUX'


E-MTAB-12386:Mock - cytoplasm

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12386:Mock - cytoplasm

tag: Namespace:Centro de Investigacion en Dinamica Celular,  Universidad Autonoma de Morelos

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fibroblast of lung

text: human

characteristic: common name

text: Protocols: Human lung carcinoma A549 cells (ATCC CCL-185; American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle medium (Sigma, St. Louis, MO, USA) supplemented with 0.11 g/L sodium pyruvate, 10% fetal bovine serum (PAN Biotech, Aidenbach, Germany) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin, PAN Biotech), and maintained at 37°C in a 5% CO2 atmosphere. Cells were routinely checked for mycoplasma contamination. For infections, the HAdV-C5 H5pg4100 virus was used, which has a deletion in the E3 region but a wild type phenotype in cell culture. Subconfluent A549 cells were infected at a multiplicity of infection of 50 fluorescence-forming units/cell for 6 to 48 h in all experiments. Mock-infected and HAdV-C5-infected cells (~2.5 × 10^6 per condition) were harvested by trypsinization (at 6, 12, 24 and 48 h p.i.), washed, pelleted and resuspended in 100 µl cold NP-40 lysis buffer (10 mM HEPES (pH 7.8), 10 mM KCl, 20% (v/v) glycerol, 0.25% (v/v) NP-40 and 1 mM DTT) and incubated on ice during 2 min. Samples were centrifuged (470 × g, 5 min at 4ºC) and the supernatants (~80 μl) were transferred into a new tube containing 600 μl TRIzol (Invitrogen, Carlsbad, CA, USA) and stored as the cytoplasmic fraction at -20°C. The remaining nuclear pellets were washed with cold NP-40 lysis buffer, centrifuged again and the supernatants were discarded. These residual pellets, which contain the cell nuclei were resuspended in 600 μl of TRIzol and stored as the nucleo-plasmic fraction at -20°C until further use. After collecting all samples, they were thawed and incubated for 5 min at room tem-perature, before 120 µl chloroform was added, tubes were shaken for 15 sec and incubated for 2-3 min at room temperature. Next, all samples were centrifuged (12,000 × g, 15 min at 4ºC) and the upper aqueous phase was transferred into a new tube. RNAs were pre-cipitated from these samples by adding 1.5 times the volume of 100% ethanol. From this mixture, 700 µl were transferred to RNA extraction columns (RNeasy Plus Mini Kit (Qiagen, Hilden, Germany)) and further processed according to the manufacturer’s in-structions. Final RNA concentrations were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and samples were stored at -80°C. RNA samples from both cytoplasmic and nucleoplasmic fractions were sent to the Leibniz Institute of Virology NGS technology platform. Here, the RNA quality was evaluated using a Bioanalyzer with the RNA Nano Chip (Agilent Technologies, Böblingen, Germany). To isolate mRNAs from the total RNA sample, a poly(A) selection was performed using the NEBnext poly(A) mRNA Magnetic Isolation Module (NEB sequencing, Ipswitch, MA, USA). The cDNA libraries were generated with the ScriptSeq v2 RNAseq Kit (Epicentre Biotechnologies, Madison, WI, USA) using their strand-specific protocol where forward strands were labeled. The sizes and qualities of the libraries were controlled using the Bioanalyzer High Sensitivity DNA Chip Kit (Agilent Technologies).

characteristic: description

text: adult

characteristic: developmental stage

text: infectious disease

characteristic: disease

text: cytoplasm

characteristic: fraction

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Mock - cytoplasm

characteristic: title


E-MTAB-12386:Mock - nucleoplasm

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Centro de Investigacion en Dinamica Celular, Universidad Autonoma de Morelos

characteristic: INSDC center name

text: public

text: E-MTAB-12386:Mock - nucleoplasm

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: nucleoplasm

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: Mock - nucleoplasm


E-MTAB-12386:6 hpi - cytoplasm

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-12386:6 hpi - cytoplasm

characteristic: Submitter Id

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: cytoplasm

text: Human Adenovirus 5

characteristic: infect

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 6 hpi - cytoplasm


E-MTAB-12386:6 hpi - nucleoplasm

text: public

characteristic: SRA accession

text: E-MTAB-12386:6 hpi - nucleoplasm

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fibroblast of lung

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: infectious disease

text: nucleoplasm

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 6 hpi - nucleoplasm


E-MTAB-12386:12 hpi - cytoplasm

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12386:12 hpi - cytoplasm

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

characteristic: disease

text: cytoplasm

characteristic: fraction

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: 12 hpi - cytoplasm

characteristic: title


E-MTAB-12386:12 hpi - nucleoplasm

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-12386:12 hpi - nucleoplasm

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: nucleoplasm

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 12 hpi - nucleoplasm


E-MTAB-12386:24 hpi - cytoplasm

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12386:24 hpi - cytoplasm

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: cytoplasm

text: Human Adenovirus 5

characteristic: infect

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 24 hpi - cytoplasm


E-MTAB-12386:24 hpi - nucleoplasm

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-12386:24 hpi - nucleoplasm

text: ArrayExpress

characteristic: broker name

text: fibroblast of lung

text: human

characteristic: common name

characteristic: description

text: adult

characteristic: developmental stage

text: infectious disease

characteristic: disease

text: nucleoplasm

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 24 hpi - nucleoplasm


E-MTAB-12386:48 hpi - cytoplasm

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: E-MTAB-12386:48 hpi - cytoplasm

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: cytoplasm

characteristic: fraction

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: 48 hpi - cytoplasm

characteristic: title


E-MTAB-12386:48 hpi - nucleoplasm

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12386:48 hpi - nucleoplasm

text: ArrayExpress

text: fibroblast of lung

text: human

text: adult

text: infectious disease

text: nucleoplasm

text: Human Adenovirus 5

text: Homo sapiens

text: Homo sapiens

text: lung

text: Homo sapiens

text: male

text: 48 hpi - nucleoplasm


characteristic: ENA-CHECKLIST

characteristic: GISAID Accession ID

text: public

tag: Namespace:Institute for Infectious Diseases, Bern, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: MCL Medizinische Laboratorien, Hauptstandort Niederwangen; Niederwangen, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Lea Stauber, Christian Baumann, Miguel A Terrazos Miani, Sonja Gempeler, Loïc Borcard, Stefan Neuenschwander, Cora Sägesser, Pascal Bittel, Peter Keller, Franziska Suter-Riniker, Stephen L Leib, Alban Ramette

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BE

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-IFIK-9208-6597/2022

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

text: active surveillance in response to outbreak

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Microbiota Center Amsterdam

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Microbiota Center Amsterdam

characteristic: description

text: human metagenome

text: human metagenome


characteristic: GISAID Accession ID

text: public

tag: Namespace:Institute of Medical Virology, Zürich, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Stadtspital Triemli, Zürich, Switzerland

characteristic: collecting institution

characteristic: collection date

text: Verena Kufner, Maryam Zaheri, Stefan Schmutz, Kevin Steiner, Riccarda Capaul, Guido Bloemberg, Michael Huber, Alexandra Trkola, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

characteristic: collector name

text: Switzerland

characteristic: geographic location (country and/or sea)

text: ZH

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba677c5/2022

text: active surveillance not initiated by an outbreak

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

text: public

tag: Namespace:ETH ZURICH D-BSSE

text: SIB

text: Viollier AG; Hagmattstrasse 14, 4123 Allschwil

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-ETHZ-38006100/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-38006057/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Medical Virology, Zürich, Switzerland

characteristic: INSDC center name

text: public

text: SIB

text: Stadtspital Waid, Zürich, Switzerland

text: Switzerland

characteristic: geographic location (country and/or sea)

text: ZH

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba6779c/2022

characteristic: title


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: Switzerland

text: BE

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/BE-ETHZ-38006091/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

tag: Namespace:Laboratorio di Microbiologia, Dipartimento di Medicina di Laboratorio EOLAB, Ente Ospedaliero Cantonale, Bellinzona, Switzerland

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: EOC Bellinzona; Via Mirasole 22/A, 6500 Bellinzona

characteristic: collecting institution

characteristic: collection date

text: Spina Valeria Romina, Gloria Gaudio, Gladys Martinetti Lucchini, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

characteristic: collector name

text: Switzerland

text: TI

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/TI-EOC-38006145/2022


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: Switzerland

characteristic: geographic location (country and/or sea)

text: ZH

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-ETHZ-38006070/2022

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: SIB

text: Universität Zürich, Zürich, Switzerland

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba677df/2022

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: sample capture status


characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Universitäts-Kinderspital Zürich, Zürich, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba6782a/2022

text: active surveillance in response to outbreak


characteristic: ENA-CHECKLIST

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characteristic: External Id

text: ETH ZURICH D-BSSE

text: public

characteristic: INSDC status

text: SIB

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BS

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-38006050/2022

text: active surveillance not initiated by an outbreak

characteristic: title


characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Universität Zürich, Zürich, Switzerland

text: Verena Kufner, Maryam Zaheri, Stefan Schmutz, Kevin Steiner, Riccarda Capaul, Guido Bloemberg, Michael Huber, Alexandra Trkola, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba677dc/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Stadtspital Waid, Zürich, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba67800/2022


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: Viollier AG; Hagmattstrasse 14, 4123 Allschwil

text: Andrea Patrizia Salzmann, Henriette Kurth, Christiane Beckmann, Maurice Redondo, Olivier Kobel, Christoph Noppen, Sebastian Kurscheid, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

characteristic: geographic location (country and/or sea)

text: GR

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/GR-ETHZ-38006054/2022

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Laboratorio di Microbiologia, Dipartimento di Medicina di Laboratorio EOLAB, Ente Ospedaliero Cantonale, Bellinzona, Switzerland

text: public

text: SIB

text: EOC Bellinzona; Via Mirasole 22/A, 6500 Bellinzona

text: Switzerland

text: TI

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/TI-EOC-38006135/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: BS

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BS-ETHZ-38006048/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

text: public

characteristic: INSDC status

text: SIB

text: Switzerland

characteristic: geographic location (country and/or sea)

text: BL

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/BL-ETHZ-38006090/2022

characteristic: title


characteristic: GISAID Accession ID

text: Institute of Medical Virology, Zürich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: SIB

text: Stadtspital Waid, Zürich, Switzerland

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba676b7/2022

characteristic: isolate

text: Severe acute respiratory syndrome coronavirus 2

characteristic: organism

characteristic: sample capture status


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Universität Zürich, Zürich, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-46de40c8/2022

text: active surveillance in response to outbreak


characteristic: GISAID Accession ID

text: ETH ZURICH D-BSSE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: SIB

text: Switzerland

characteristic: geographic location (country and/or sea)

text: GR

characteristic: geographic location (region and locality)

text: Human

characteristic: host common name

text: restricted access

characteristic: host health state

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/GR-ETHZ-38006014/2022

text: active surveillance not initiated by an outbreak

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: SIB

text: Universität Zürich, Zürich, Switzerland

text: Verena Kufner, Maryam Zaheri, Stefan Schmutz, Kevin Steiner, Riccarda Capaul, Guido Bloemberg, Michael Huber, Alexandra Trkola, Christian Beisel, Tobias Schär, Ina Nissen, Natascha Santacroce, Rebecca Denes, Mirjam Feldkamp, Elodie Burcklen, Chaoran Chen, Sarah Nadeau, Louis du Plessis, Ivan Topolsky, Kim Philipp Jablonski, Lara Fuhrmann, David Dreifuss, Pelin Burcak Icer, Matteo Carrara, Shuqing Yu, Franziska Singer, Niko Beerenwinkel, Tanja Stadler

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

characteristic: host scientific name

text: restricted access

characteristic: host sex

text: restricted access

characteristic: host subject id

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba67840/2022

characteristic: isolate

characteristic: organism

characteristic: sample capture status


characteristic: GISAID Accession ID

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: SIB

characteristic: broker name

text: Stadtspital Triemli, Zürich, Switzerland

characteristic: collecting institution

characteristic: collection date

characteristic: collector name

text: Switzerland

text: ZH

text: Human

text: restricted access

text: Homo sapiens

text: restricted access

text: restricted access

text: hCoV-19/Switzerland/ZH-UZH-IMV-3ba676a4/2022


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH ZURICH D-BSSE

text: public

characteristic: INSDC status

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