E-MTAB-12238:Sample 263

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 263

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 263

characteristic: title

text: human CD45 positive out of mouse bone marrow 82


E-MTAB-12238:Sample 264

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 264

tag: Namespace:Department of Molecular Cell Biology, Weizmann Institute of Science

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 264

characteristic: xenograft


E-MTAB-12238:Sample 265

characteristic: INSDC last update

text: public

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text: E-MTAB-12238:Sample 265

characteristic: Submitter Id

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text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 265


E-MTAB-12238:Sample 266

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text: E-MTAB-12238:Sample 266

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: date

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text: adult

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text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 266

characteristic: title


E-MTAB-12238:Sample 267

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text: E-MTAB-12238:Sample 267

characteristic: Submitter Id

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text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: common name

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive ; CD34 positive

characteristic: subclone

text: Sample 267


E-MTAB-12238:Sample 268

characteristic: ENA-CHECKLIST

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text: E-MTAB-12238:Sample 268

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: date

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: time

text: Sample 268


E-MTAB-12238:Sample 269

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 269

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: Miltenyi column -CD34+ Enrichment by magnetic beads

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 269

characteristic: title


E-MTAB-12238:Sample 27

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characteristic: INSDC center name

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text: public

text: E-MTAB-12238:Sample 27

text: Repli G

text: ArrayExpress

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text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD45 positive

characteristic: subclone

text: Sample 27

text: human CD45 positive out of mouse bone marrow 81

characteristic: xenograft


E-MTAB-12238:Sample 270

characteristic: INSDC last update

text: public

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text: E-MTAB-12238:Sample 270

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text: ArrayExpress

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text: acute myeloid leukemia

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text: acute myeloid leukemia

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text: Homo sapiens

text: bone marrow

characteristic: organism part

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text: Homo sapiens

text: male

characteristic: time

text: Sample 270


E-MTAB-12238:Sample 271

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text: E-MTAB-12238:Sample 271

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

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text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: female

characteristic: sex

text: IDH1(Rx); CD45 positive

text: Sample 271

characteristic: title

text: human CD45 positive out of mouse bone marrow 127


E-MTAB-12238:Sample 272

characteristic: INSDC last update

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text: E-MTAB-12238:Sample 272

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characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 272

characteristic: xenograft


E-MTAB-12238:Sample 273

characteristic: ENA-CHECKLIST

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text: E-MTAB-12238:Sample 273

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive

characteristic: time

text: Sample 273

text: 1:10 human CD45 positive out of mouse bone marrow 82


E-MTAB-12238:Sample 274

characteristic: INSDC last update

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text: E-MTAB-12238:Sample 274

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD34 positive

text: Sample 274

characteristic: title


E-MTAB-12238:Sample 275

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 275

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 275


E-MTAB-12238:Sample 276

characteristic: INSDC last update

text: public

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characteristic: SRA accession

text: E-MTAB-12238:Sample 276

characteristic: Submitter Id

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text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 276


E-MTAB-12238:Sample 277

characteristic: ENA-CHECKLIST

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text: E-MTAB-12238:Sample 277

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

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characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 277

characteristic: title


E-MTAB-12238:Sample 278

characteristic: INSDC last update

text: public

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text: E-MTAB-12238:Sample 278

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 278


E-MTAB-12238:Sample 279

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 279

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 279

text: 1:10 human CD45 positive out of mouse bone marrow 127

characteristic: xenograft


E-MTAB-12238:Sample 28

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text: public

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text: E-MTAB-12238:Sample 28

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 28

characteristic: title


E-MTAB-12238:Sample 280

characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 280

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 280


E-MTAB-12238:Sample 281

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 281

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: time

text: Sample 281

text: human CD45 positive out of mouse bone marrow 83

characteristic: xenograft


E-MTAB-12238:Sample 282

characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 282

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 282

characteristic: title


E-MTAB-12238:Sample 283

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 283

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 283


E-MTAB-12238:Sample 284

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 284

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 284


E-MTAB-12238:Sample 285

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 285

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 285

characteristic: title


E-MTAB-12238:Sample 286

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 286

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 286


E-MTAB-12238:Sample 287

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 287

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 287

text: human CD45 positive out of mouse bone marrow 130

characteristic: xenograft


E-MTAB-12238:Sample 288

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 288

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: female

characteristic: sex

text: IDH1(Rx); CD45 positive

text: Sample 288

characteristic: title


E-MTAB-12238:Sample 289

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 289

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 289

text: 1:10 human CD45 positive out of mouse bone marrow 83


E-MTAB-12238:Sample 29

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 29

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra-

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 29


E-MTAB-12238:Sample 290

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 290

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 290

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 291

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 291

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

characteristic: protocol

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 291


E-MTAB-12238:Sample 292

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 292

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: sample id

text: Homo sapiens

text: male

characteristic: time

text: Sample 292


E-MTAB-12238:Sample 293

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 293

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 293

characteristic: title


E-MTAB-12238:Sample 294

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 294

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 294


E-MTAB-12238:Sample 295

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 295

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 295


E-MTAB-12238:Sample 296

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 296

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 296

characteristic: title


E-MTAB-12238:Sample 297

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 297

text: Repli G

text: ArrayExpress

text: B cell

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 297

text: 1:10 human CD45 positive out of mouse bone marrow 130

characteristic: xenograft


E-MTAB-12238:Sample 298

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 298

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 298


E-MTAB-12238:Sample 299

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 299

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 299

characteristic: title

text: human CD45 positive out of mouse bone marrow 84


E-MTAB-12238:Sample 3

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 3

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 positive ; CD34 positive

characteristic: subclone

text: Sample 3


E-MTAB-12238:Sample 30

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 30

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 30


E-MTAB-12238:Sample 300

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 300

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 300

characteristic: title

characteristic: xenograft


E-MTAB-12238:Sample 301

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 301

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 301


E-MTAB-12238:Sample 302

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 302

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 302


E-MTAB-12238:Sample 303

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 303

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 303

characteristic: title


E-MTAB-12238:Sample 304

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 304

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 304


E-MTAB-12238:Sample 305

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 305

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: female

characteristic: sex

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 305

text: human CD45 positive out of mouse bone marrow 132

characteristic: xenograft


E-MTAB-12238:Sample 306

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 306

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: relapse

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

text: Sample 306

characteristic: title


E-MTAB-12238:Sample 307

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 307

text: Repli G

text: ArrayExpress

text: B cell

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 307

text: 1:10 human CD45 positive out of mouse bone marrow 84


E-MTAB-12238:Sample 308

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 308

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 308

characteristic: xenograft


E-MTAB-12238:Sample 309

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 309

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 309

characteristic: title


E-MTAB-12238:Sample 31

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 31

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: cell count

text: B cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 31

text: 1:10 human CD45 positive out of mouse bone marrow 15


E-MTAB-12238:Sample 310

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 310

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 310


E-MTAB-12238:Sample 311

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 311

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: granulocyte monocyte progenitor cell

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

text: Sample 311

characteristic: title


E-MTAB-12238:Sample 312

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 312

text: ArrayExpress

text: granulocyte monocyte progenitor cell

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 312


E-MTAB-12238:Sample 313

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 313

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 313


E-MTAB-12238:Sample 314

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 314

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 314

characteristic: title


E-MTAB-12238:Sample 315

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 315

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: female

characteristic: sex

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 315

text: 1:10 human CD45 positive out of mouse bone marrow 132

characteristic: xenograft


E-MTAB-12238:Sample 316

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 316

text: Repli G

text: ArrayExpress

text: B cell

text: relapse

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 316


E-MTAB-12238:Sample 317

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 317

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 317

characteristic: title

text: human CD45 positive out of mouse bone marrow 85


E-MTAB-12238:Sample 318

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 318

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 318

characteristic: xenograft


E-MTAB-12238:Sample 319

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 319

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 319


E-MTAB-12238:Sample 32

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 32

text: Repli G

text: ArrayExpress

characteristic: cell count

text: B cell

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 32

characteristic: title


E-MTAB-12238:Sample 320

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 320

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 320


E-MTAB-12238:Sample 321

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 321

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD34 positive ; CD34 positive

characteristic: time

text: Sample 321


E-MTAB-12238:Sample 322

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 322

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: Sample 322

characteristic: title


E-MTAB-12238:Sample 323

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 323

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 323

text: human CD45 positive out of mouse bone marrow 137

characteristic: xenograft


E-MTAB-12238:Sample 324

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 324

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: relapse

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 324


E-MTAB-12238:Sample 325

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 325

text: Repli G

text: ArrayExpress

text: B cell

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 325

characteristic: title

text: 1:10 human CD45 positive out of mouse bone marrow 85


E-MTAB-12238:Sample 326

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 326

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 326

characteristic: xenograft


E-MTAB-12238:Sample 327

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 327

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 327


E-MTAB-12238:Sample 328

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 328

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+

text: Sample 328

characteristic: title


E-MTAB-12238:Sample 329

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 329

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 329


E-MTAB-12238:Sample 33

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 33

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 33

text: 1:10 human CD45 positive out of mouse bone marrow 81

characteristic: xenograft


E-MTAB-12238:Sample 330

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 330

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: Sample 330

characteristic: title


E-MTAB-12238:Sample 331

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 331

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: B cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: subclone

text: Sample 331

text: 1:10 human CD45 positive out of mouse bone marrow 137


E-MTAB-12238:Sample 332

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 332

text: Repli G

text: ArrayExpress

text: B cell

text: relapse

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: female

text: IDH1(Rx); CD45 positive

characteristic: time

text: Sample 332

characteristic: xenograft


E-MTAB-12238:Sample 333

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 333

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: blood

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: NPM1+ FLT3-ITD+; CD45 positive

text: Sample 333

characteristic: title

text: human CD45 positive out of mouse bone marrow 86


E-MTAB-12238:Sample 334

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 334

text: Repli G

text: ArrayExpress

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+; CD45 positive

characteristic: subclone

text: Sample 334


E-MTAB-12238:Sample 335

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 335

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: hematopoietic multipotent progenitor cell

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 335


E-MTAB-12238:Sample 336

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 336

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 336

characteristic: title


E-MTAB-12238:Sample 337

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 337

characteristic: Submitter Id

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common lymphoid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra+

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 337


E-MTAB-12238:Sample 338

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 338

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 338


E-MTAB-12238:Sample 339

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 339

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38+, CD10-, CD7-, CD135(FLT3)+, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 339

characteristic: title


E-MTAB-12238:Sample 34

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 34

text: Repli G

text: ArrayExpress

text: common lymphoid progenitor

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra+

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 34

characteristic: xenograft


E-MTAB-12238:Sample 340

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 340

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 340


E-MTAB-12238:Sample 341

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 341

text: Repli G

text: ArrayExpress

text: relapse

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 341

characteristic: title


E-MTAB-12238:Sample 342

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 342

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: relapse

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 342


E-MTAB-12238:Sample 343

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 343

text: Repli G

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD34 negative; CD34 negative

characteristic: time

text: Sample 343


E-MTAB-12238:Sample 344

text: Department of Molecular Cell Biology, Weizmann Institute of Science

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 344

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: Sample 344

characteristic: title


E-MTAB-12238:Sample 345

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 345

text: Repli G

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 345


E-MTAB-12238:Sample 346

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 346

characteristic: Submitter Id

text: Repli G

characteristic: WGA

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia

characteristic: clinical information

text: human

characteristic: description

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 346


E-MTAB-12238:Sample 347

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 347

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

characteristic: date

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: DNA Purification from BM

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 347

characteristic: title


E-MTAB-12238:Sample 348

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 348

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: DNA Purification from BM

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: subclone

text: Sample 348


E-MTAB-12238:Sample 349

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 349

text: Repli G

characteristic: WGA

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: time

text: Sample 349


E-MTAB-12238:Sample 35

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 35

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: common myeloid progenitor

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria - H4100 colony

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 35

characteristic: title


E-MTAB-12238:Sample 350

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 350

text: Repli G

characteristic: WGA

text: ArrayExpress

text: common myeloid progenitor

text: diagnosis

text: acute myeloid leukemia

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

characteristic: immunophenotype

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

text: NPM1+ FLT3-ITD+

characteristic: subclone

text: Sample 350


E-MTAB-12238:Sample 351

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 351

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 351


E-MTAB-12238:Sample 353

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 353

text: gene all kit

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: blood

text: FACS Aria

characteristic: sample id

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-; CD45 positive

text: Sample 353

characteristic: title

text: human CD45 positive out of mouse bone marrow 12L

characteristic: xenograft


E-MTAB-12238:Sample 354

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 354

characteristic: Submitter Id

text: gene all kit

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

text: Protocols: CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice. xenograft protocol: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were maintained and bred under specific pathogen-free conditions at the Biomedical Core Facility (Faculty of Medicine, the Technion, Haifa, Israel). Twenty-four hours prior to transplantation, 8-12-week-old female NSG mice were sub-lethally irradiated with 225 cGy. Primary human cells were intra-femorally injected. The total number of cells transplanted per mouse depended on the number of viable cells obtained from the BM/PB of a patient [ranged from 1 colony (a single cell-derived subclone) to 106 cells]. The animals were sacrificed 8-30 weeks after transplantation or if tumors developed in the injected knee. The BM was then harvested. Human engraftment was evaluated by flow cytometry using the following anti-human specific antibodies: anti-CD45-APC Cyanine7 (APC-Cy7) (BioLegend #304014 clone H130); anti-CD33-BV510 (BioLegend #366610); anti-CD34-BV421 (BioLegend #343610); anti-CD38-FITC (BD #555459); anti-CD15 APC (BD #551376); anti-CD19 PE-CY7 (BD #557835); Cell viability was obtained using Propidium Iodide - PI (BioLegend #421301). Analyses were performed using FlowJo software v.10 (Treestar, OR, USA), and the expression of markers was assessed and presented as the percentage of stained cells. The threshold for engraftment detection was 0.1% of human CD45+ cells. All flow cytometry analyses were done using an LSR II (BD Bioscience, CA, USA) or FACSariaII (BD Bioscience, CA, USA) device. Engraftment of >5% of hCD45+ cells with the major CD33+ population (>60% of the hCD45+ cells) was classified as overt leukemia; the presence of 0.1-5% of hCD45+ cells or the presence of >5% of hCD45+ cells with <60% of the CD33+ population in the hCD45+ cells was classified as non-overt leukemia; and the presence of <0.1% of human CD45+ (hCD45+) population was considered as non-engraftment. hCD45+ cells were sorted and underwent whole genome amplification (WGA) for further genotyping assays. CD34+ cells were isolated from PB or BM (following mononuclear cell separation) of AML patients by positive selection using anti-human CD34 microbeads. Enriched CD34+ cells were sorted by FACSariaII (BD bioscience, CA, USA) into four subpopulations of HSPCs according to their surface markers and CD33 expression. The sorted fractions were collected either into Iscove's modified Dulbecco's medium (IMDM) +20% fetal bovine serum (FBS) (Biological Industries #02-023-1A) to be further used in the colony forming unit (CFU) assay or into PBS+2%FBS to be used in the xenotransplantation assay. The CFU assay was adjusted to the generation of single cell-derived subclones. HSPCs were seeded in 1% standard methylcellulose medium supplemented with 37% FBS, 1.2% bovine serum albumin (BSA), 1% L-glutamine, 10-6M b-mercaptoethanol, 1 IU/ml of human erythropoietin (EPO), 50 ng/ml of human stem cell factor (SCF), 25 ng/ml of human FLT3 ligand, 10 ng/ml of interleukin 3 (IL-3), 10 ng/ml of interleukin 6 (IL-6), 10 ng/ml of granulocytecolony stimulating factor (G-CSF), 5 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of human thrombopoietin (TPO), 100 IU ml penicillin and 100 mg/ml streptomycin. Colonies were counted after 14 days and each of them was picked under sterile conditions. Every individual colony was divided into two cell suspensions to perform both genotyping analysis and xenotransplantation into NSG mice One µl of DNA template was added to a hybridization mix together with an MIP pool (final concentration of 0.05pM per probe) in 1x Ampligase buffer (Epicentre). The mix was incubated in a thermal cycler according to the following program: 98°C for 3 minutes, 85°C for 30 minutes, 60°C for 60 minutes and 56°C for 1 or 2 overnight incubations. The product was mixed with (final concentration in brackets): dNTPs (Larova, 15pM), Betaine (375 mM, Sigma-Aldrich,), NAD+ (1 mM, New England Biolabs), additional Ampligase buffer (0.5x), Ampligase (total of 1.25U, Epicentre) and Phusion HF (0.16U, New England Biolabs). The mix was incubated at 56°C for 60 minutes followed by 72°C for 20 minutes. Enzymatic digestion of linear probes was performed by adding Exonuclease I (4U, New England Biolabs) and Exonuclease III (25U, New England Biolabs). The mix was incubated at 37°C for 2 hours, followed by 80°C for 20 minutes. The final product was amplified using iProof HF Master Mix (Biorad). Samples were pooled and concentrated using AMPure XP beads (Beckman Coulter) at 1.3x volumetric concentration, size-selected (190-370bp) using Blue Pippin (Sage scientific), and sequenced in either NextSeq or Novaseq6000 (Illumina) 2X151bp paired-end run using custom primers as described in the past(Biezuner et al., 2022)

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

characteristic: individual

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: blood

text: FACS Aria

text: Homo sapiens

text: male

characteristic: subclone

text: Sample 354


E-MTAB-12238:Sample 355

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-12238:Sample 355

text: Repli G

characteristic: WGA

text: ArrayExpress

text: diagnosis

text: acute myeloid leukemia B phenotype

text: human

characteristic: common name

text: adult

characteristic: developmental stage

text: acute myeloid leukemia

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: immunophenotype

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: FACS Aria

characteristic: protocol

characteristic: sample id

text: Homo sapiens

text: male

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

characteristic: time

text: Sample 355


E-MTAB-12238:Sample 356

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-12238:Sample 356

characteristic: Submitter Id

text: Repli G

text: ArrayExpress

characteristic: broker name

text: diagnosis

characteristic: clinical history

text: acute myeloid leukemia B phenotype

characteristic: clinical information

text: human

characteristic: date

characteristic: description

text: adult

text: acute myeloid leukemia

characteristic: disease

text: CD34+, CD38-,CD90+/-, CD45Ra-

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: FACS Aria

text: Homo sapiens

text: male

characteristic: sex

text: BCR-ABL/NPM1-/FLT3 ITD-/DNMT3A-

text: Sample 356

characteristic: title