E-MTAB-11319:Sample 52-pt57

characteristic: ENA-CHECKLIST

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11319:Sample 52-pt57

text: ArrayExpress

text: bone marrow cell

text: human

text: juvenile stage

text: childhood acute lymphoblastic leukemia

text: diagnosis

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 52-pt57


E-MTAB-11319:Sample 39-pt44

status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: INSDC status

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text: E-MTAB-11319:Sample 39-pt44

tag: Namespace:Centro Ricerca M. Tettamanti, University of Milano-Bicocca

characteristic: Submitter Id

unit: year

characteristic: age

text: ArrayExpress

characteristic: broker name

text: bone marrow cell

text: human

characteristic: common name

text: Protocols: Mononuclear cell by ficoll density purification of Bone Marrow aspirates RNA was isolated using guanidinium thiocyanate-phenol-chloroform extraction protocol For library preparation we used the Ovation Target Enrichment System (Nugen, Tecan Genomics, CA, USA) or Trusight Pancancer kit (Illumina, CA, USA), which provide a complete custom target enrichment and multiplex library preparation solution for targeted resequencing of 95 and 1385 genes, respectively. We started from 100-200ng of total RNA of mononucleate cells from bone marrow of tumor diagnosis. The protocol started processing samples as single-tube workflow to obtain double-stranded cDNA, end repaired and individually ligated with barcoded adaptors single sample by using single-index barcoding. We performed an optional normalization step to balance the input into the enrichment step and we proceeded to sample multiplexing (8-plex). Following a bead purification step, samples are combined for multiplex target enrichment during which time the targeting probes are annealed and extended, creating amplifiable library molecules. Following a second bead purification, an optional qPCR step determined the optimal number of PCR cycles to use in the final library amplification step. After library amplification, a final bead purification prepares the multiplexed, target enriched library for sequencing. We analyzed the final library by Qubit 4.0 fluorimeter and Agilent Bioanalyzer.

characteristic: description

text: juvenile stage

characteristic: developmental stage

text: childhood acute lymphoblastic leukemia

characteristic: disease

text: diagnosis

characteristic: disease staging

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: bone marrow

text: Homo sapiens

text: male

text: Sample 39-pt44

characteristic: title


E-MTAB-11319:Sample 73-pt131

characteristic: ENA-CHECKLIST

text: Centro Ricerca M. Tettamanti, University of Milano-Bicocca

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-11319:Sample 73-pt131

text: ArrayExpress

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text: diagnosis

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text: bone marrow

characteristic: organism part

text: Homo sapiens

text: male

characteristic: sex

text: Sample 73-pt131


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characteristic: ENA-LAST-UPDATE

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characteristic: INSDC center name

text: public

characteristic: INSDC status

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text: 61355:90342130-7e31-4546-b51a-ee80def6f193

tag: Namespace:Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

text: Water

characteristic: Water environmental package

text: GFBIO

text: coastal water with size fractionation >20um (Microplankton)

text: marine biome [ENVO:00000447] | polar biome [ENVO:01000339]

characteristic: environment (biome)

text: microbial feature [ENVO:01000007] | marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System. libraries: Primers were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/). Primer targeting the V4F for the 18S rRNA gene (Stoeck et al. 2010)

text: marine metagenome

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

characteristic: submitted to insdc


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text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

characteristic: INSDC first public

characteristic: INSDC last update

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text: 61354:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

text: GFBIO

characteristic: broker name

characteristic: description

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

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text: 61353:90342130-7e31-4546-b51a-ee80def6f193

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characteristic: Water environmental package

text: GFBIO

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


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text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

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text: Water

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text: GFBIO

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text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

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characteristic: organism

text: marine metagenome

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text: true

characteristic: title


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text: 61350:90342130-7e31-4546-b51a-ee80def6f193

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text: GFBIO

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

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text: Illumina MiSeq

text: true


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text: Water

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text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

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text: mimarks-survey

characteristic: investigation type

text: marine metagenome

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text: marine metagenome

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text: coastal sea water [ENVO:00002150]

text: Water

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text: marine metagenome

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text: true

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text: Water

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text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


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text: 61346:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

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text: true

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text: 61345:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


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text: public

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text: 61343:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

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text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

characteristic: project name

text: marine metagenome

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text: true

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characteristic: INSDC first public

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text: public

text: 61342:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal water with size fractionation >20um (Microplankton)

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: INSDC center name

text: public

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text: 61341:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

text: marine biome [ENVO:00000447] | polar biome [ENVO:01000339]

text: microbial feature [ENVO:01000007] | marine photic zone [ENVO:00000209]

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


characteristic: ENA-CHECKLIST

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text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

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characteristic: INSDC last update

text: public

text: 61340:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System. libraries: Primers were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/). Primer targeting the V4F for the 18S rRNA gene (Stoeck et al. 2010)

text: marine metagenome

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

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text: 61339:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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text: public

text: 61338:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


characteristic: INSDC center name

text: public

characteristic: INSDC status

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text: 61337:90342130-7e31-4546-b51a-ee80def6f193

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text: Water

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text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

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text: mimarks-survey

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text: marine metagenome

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text: true

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text: 61336:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

characteristic: description

characteristic: environment (biome)

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text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

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text: marine metagenome

text: Illumina MiSeq

text: true

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text: public

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text: 61335:90342130-7e31-4546-b51a-ee80def6f193

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text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

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characteristic: INSDC last update

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text: 61334:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

characteristic: project name

text: marine metagenome

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text: true

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text: Water

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text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

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characteristic: organism

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text: Illumina MiSeq

text: true

characteristic: title


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text: GFBIO

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text: Water

characteristic: environmental package

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characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

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text: Water

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text: GFBIO

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text: Water

text: Norway

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text: Water

text: GFBIO

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

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characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

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text: Water

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text: GFBIO

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text: coastal water with size fractionation >20um (Microplankton)

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characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: marine metagenome

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text: Illumina MiSeq

text: true


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text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

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text: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research

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text: public

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text: 61327:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

characteristic: description

characteristic: environment (biome)

text: microbial feature [ENVO:01000007] | marine photic zone [ENVO:00000209]

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: public

text: 61326:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

characteristic: environmental package

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

text: mimarks-survey

text: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System. libraries: Primers were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/). Primer targeting the V4F for the 18S rRNA gene (Stoeck et al. 2010)

text: marine metagenome

text: marine metagenome

text: Illumina MiSeq

text: true


characteristic: INSDC center name

text: public

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text: 61325:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: marine metagenome

characteristic: project name

text: marine metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: true

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

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text: public

text: 61324:90342130-7e31-4546-b51a-ee80def6f193

text: Water

text: GFBIO

characteristic: description

characteristic: environment (biome)

characteristic: environment (feature)

text: coastal sea water [ENVO:00002150]

text: Water

text: Norway

text: mimarks-survey

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Illumina MiSeq

text: true

characteristic: title


characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 61323:90342130-7e31-4546-b51a-ee80def6f193

characteristic: Submitter Id

text: Water

characteristic: Water environmental package

text: GFBIO

characteristic: broker name

text: coastal sea water [ENVO:00002150]

characteristic: environment (material)

text: Water

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